Rroxscaffold_6G00425380

Cleavage and polyadenylation specificity factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0013739
Physical Location & Seq
Reverse (-)
46342096 .. 46356593
14498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00425380.1

Sequence Viewer

Length: 1125 bp
ATGAATCCAGTAAGTACTTACCTTATGTATGGAATCGGTGGGGCTGCTACAGGTGGTCTCATCATCATGAAATACGATGATTATGTCGCTATGGGAAGACAAGTAAATGAGTTGATATCGAAACAGATCGATCCAAAGATTGAAGAGATACGGAGAGTTTGGAGGCCAGAAGAATTTGAAGCATTTCATCCTGATTACAAAGACATCTTCCTCGAAAGATATGACCAGTGGAAGAAGAGGATGACTACTCCTTCCATGTTTCGACAGTGGAGAGATGCATTTCGGTCATTACGGTGCAGCAACTGCAATTGCGATTCATCCAACAGCTCCGGAATCGAGAACGAATCCCACCATCGCCAGACCTTCACTACGCTTAGACCTCGCCGGACATTCACCGCATCCACAACCCGCCGGACCGTCACCGCCCCGACAGATCAAGAAGTTGTAGTACAAGAAATTATTGTGATACATAGAGATAGACCACCTATACTAAATGCAAGCCTAAATGCTAAGAAGCAGAAAGGCAAAGTGATTTGTTTGCATTGCCGTATTCATCAATTGACTTTCAGTCCTCATACAGATGCCAAAGGGATTATGGATCTGGTGAAGTTTCTTTGTCCCAAGCATGTGATACTTGTTCATGGTGAGAAGCCTAGGATGGCTACTTCCGAAAGGAAGAATTCAATCCGAACTGGGAATTCAAAGTTGCTCAAATCCAAACTTCAAATGACTTGGAAACAAAGCCGCTTATCAGTTCTGGTCAAAGCACAGATGGAAATGGAAGCTATTGCCGTTATTGTTCTTGGTATCGGTTTAGTTGGTGGTTACCGGCAGTATCGCAAGAAGCGTGATCTGGTTTCTCTAAATAAAGATTTCGAACTTAAAAGATGGAGGCGTTTCAGAGACCCAAAACGTATTGTGCTCATCTCCAGGTCCGAAGGGATCGGAGCCACTAATCGCACTGCTACGTTAATAGCGGGATTCGACAATGAACACCGTACGGCTACGTGGGGTCATACTTGTCTTCCCGGCAATGTTCGTATATCATTTGCAGGTCACATAAAATTTCTCACATCTCTCTTTGGTCTTGGGAAGTTTTGGAACAGCGAAGAATTAGTACCTTAA

Protein Analysis

374

Amino Acids

43.08

Weight (kDa)

10.04

Isoelectric Point (pI)

46.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RMMBL PF07521 181 - 222 2.1e-10 Zn-dependent metallo-hydrolase RNA specificity domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1043
AccIII TCCGGA 1 cut(s) 329
AciI CCGC 5 cut(s) 396, 409, 423, 745, 977
AclWI GGATC 3 cut(s) 125, 606, 950
AcsI RAATTY 4 cut(s) 173, 679, 697, 1064
AfaI GTAC 4 cut(s) 16, 450, 1000, 1119
AgsI TTSAA 5 cut(s) 143, 179, 684, 702, 725
AhdI GACNNNNNGTC 1 cut(s) 567
AjnI CCWGG 1 cut(s) 929
AluBI AGCT 2 cut(s) 327, 785
AluI AGCT 2 cut(s) 327, 785
Alw21I GWGCWC 1 cut(s) 924
Alw26I GTCTC 2 cut(s) 62, 897
AlwI GGATC 3 cut(s) 125, 606, 950
AlwNI CAGNNNCTG 1 cut(s) 303
Aor13HI TCCGGA 1 cut(s) 329
AoxI GGCC 1 cut(s) 164
ApeKI GCWGC 2 cut(s) 44, 297
ApoI RAATTY 4 cut(s) 173, 679, 697, 1064
Asp700I GAANNNNTTC 1 cut(s) 183
AspA2I CCTAGG 1 cut(s) 653
AspS9I GGNCC 2 cut(s) 414, 933
AsuC2I CCSGG 1 cut(s) 1029
AsuHPI GGTGA 4 cut(s) 385, 412, 616, 656
AsuII TTCGAA 1 cut(s) 876
AvaII GGWCC 2 cut(s) 414, 933
AvrII CCTAGG 1 cut(s) 653
BarI GAAGNNNNNNTAC 3 cut(s) 432, 464, 1101
BbsI GAAGAC 2 cut(s) 103, 1016
Bbv12I GWGCWC 1 cut(s) 924
BbvI GCAGC 2 cut(s) 31, 309
BccI CCATC 4 cut(s) 360, 652, 766, 882
BceAI ACGGC 3 cut(s) 531, 776, 1017
BciT130I CCWGG 1 cut(s) 931
BcnI CCSGG 1 cut(s) 1029
BcoDI GTCTC 2 cut(s) 62, 897
BfaI CTAG 1 cut(s) 654
BfmI CTRYAG 1 cut(s) 48
BfuAI ACCTGC 1 cut(s) 1043
BisI GCNGC 3 cut(s) 45, 298, 745
BlnI CCTAGG 1 cut(s) 653
BlsI GCNGC 3 cut(s) 46, 299, 746
BmcAI AGTACT 1 cut(s) 16
Bme1390I CCNGG 2 cut(s) 931, 1029
Bme18I GGWCC 2 cut(s) 414, 933
BmeRI GACNNNNNGTC 1 cut(s) 567
BmgT120I GGNCC 2 cut(s) 414, 933
BmiI GGNNCC 1 cut(s) 949
BmrFI CCNGG 2 cut(s) 931, 1029
BmrI ACTGGG 1 cut(s) 702
BmsI GCATC 3 cut(s) 265, 407, 571
BmuI ACTGGG 1 cut(s) 702
BpiI GAAGAC 2 cut(s) 103, 1016
BpmI CTGGAG 1 cut(s) 913
Bpu14I TTCGAA 1 cut(s) 876
BpuMI CCSGG 1 cut(s) 1029
Bsa29I ATCGAT 1 cut(s) 129
BsaAI YACGTR 1 cut(s) 1008
BsaI GGTCTC 2 cut(s) 62, 897
BsaJI CCNNGG 1 cut(s) 653
BsaWI WCCGGW 1 cut(s) 329
Bse118I RCCGGY 1 cut(s) 828
Bse1I ACTGG 3 cut(s) 8, 226, 697
Bse3DI GCAATG 2 cut(s) 541, 1039
BseAI TCCGGA 1 cut(s) 329
BseBI CCWGG 1 cut(s) 931
BseCI ATCGAT 1 cut(s) 129
BseDI CCNNGG 1 cut(s) 653
BseGI GGATG 5 cut(s) 187, 246, 317, 398, 663
BseMI GCAATG 2 cut(s) 541, 1039
BseNI ACTGG 3 cut(s) 8, 226, 697
BseXI GCAGC 2 cut(s) 31, 309
BsgI GTGCAG 1 cut(s) 316
BshFI GGCC 1 cut(s) 166
BshVI ATCGAT 1 cut(s) 129
BsiHKAI GWGCWC 1 cut(s) 924
BsiSI CCGG 5 cut(s) 330, 385, 412, 829, 1029
BsiWI CGTACG 1 cut(s) 998
BslFI GGGAC 1 cut(s) 603
BsmAI GTCTC 2 cut(s) 62, 897
BsmFI GGGAC 1 cut(s) 603
BsnI GGCC 1 cut(s) 166
Bso31I GGTCTC 2 cut(s) 62, 897
Bsp119I TTCGAA 1 cut(s) 876
Bsp1286I GDGCHC 1 cut(s) 924
Bsp13I TCCGGA 1 cut(s) 329
Bsp143I GATC 6 cut(s) 126, 130, 433, 598, 850, 942
BspACI CCGC 5 cut(s) 396, 409, 423, 745, 977
BspANI GGCC 1 cut(s) 166
BspDI ATCGAT 1 cut(s) 129
BspEI TCCGGA 1 cut(s) 329
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 949
BspMI ACCTGC 1 cut(s) 1043
BspPI GGATC 3 cut(s) 125, 606, 950
BspT104I TTCGAA 1 cut(s) 876
BspTNI GGTCTC 2 cut(s) 62, 897
BsrDI GCAATG 2 cut(s) 541, 1039
BsrFI RCCGGY 1 cut(s) 828
BsrI ACTGG 3 cut(s) 8, 226, 697
BssAI RCCGGY 1 cut(s) 828
BssECI CCNNGG 1 cut(s) 653
BssMI GATC 6 cut(s) 126, 130, 433, 598, 850, 942
BssT1I CCWWGG 1 cut(s) 653
Bst2UI CCWGG 1 cut(s) 931
Bst4CI ACNGT 4 cut(s) 267, 294, 418, 998
Bst6I CTCTTC 2 cut(s) 138, 230
BstAPI GCANNNNNTGC 1 cut(s) 303
BstBAI YACGTR 1 cut(s) 1008
BstBI TTCGAA 1 cut(s) 876
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 2 cut(s) 374, 510
BstEII GGTNACC 1 cut(s) 824
BstF5I GGATG 5 cut(s) 187, 246, 317, 398, 663
BstKTI GATC 6 cut(s) 129, 133, 436, 601, 853, 945
BstMAI GTCTC 2 cut(s) 62, 897
BstMBI GATC 6 cut(s) 126, 130, 433, 598, 850, 942
BstMWI GCNNNNNNNGC 1 cut(s) 303
BstNI CCWGG 1 cut(s) 931
BstNSI RCATGY 1 cut(s) 629
BstPI GGTNACC 1 cut(s) 824
BstSCI CCNGG 2 cut(s) 929, 1027
BstSFI CTRYAG 1 cut(s) 48
BstV1I GCAGC 2 cut(s) 31, 309
BstV2I GAAGAC 2 cut(s) 103, 1016
BstX2I RGATCY 1 cut(s) 598
BstYI RGATCY 1 cut(s) 598
Bsu15I ATCGAT 1 cut(s) 129
BsuRI GGCC 1 cut(s) 166
BsuTUI ATCGAT 1 cut(s) 129
BtgZI GCGATG 1 cut(s) 338
BtsCI GGATG 5 cut(s) 187, 246, 317, 398, 663
BtsI GCAGTG 1 cut(s) 960
BtsIMutI CAGTG 3 cut(s) 233, 272, 960
BveI ACCTGC 1 cut(s) 1043
Cac8I GCNNGC 1 cut(s) 499
CaiI CAGNNNCTG 1 cut(s) 303
CciI TCATGA 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 828
Cfr13I GGNCC 2 cut(s) 414, 933
ClaI ATCGAT 1 cut(s) 129
CpoI CGGWCCG 1 cut(s) 414
Csp6I GTAC 4 cut(s) 15, 449, 999, 1118
CspI CGGWCCG 1 cut(s) 414
CviAII CATG 4 cut(s) 67, 256, 626, 641
CviQI GTAC 4 cut(s) 15, 449, 999, 1118
DdeI CTNAG 2 cut(s) 374, 510
DpnI GATC 6 cut(s) 128, 132, 435, 600, 852, 944
DpnII GATC 6 cut(s) 126, 130, 433, 598, 850, 942
DriI GACNNNNNGTC 1 cut(s) 567
Eam1104I CTCTTC 2 cut(s) 138, 230
Eam1105I GACNNNNNGTC 1 cut(s) 567
EarI CTCTTC 2 cut(s) 138, 230
Eco130I CCWWGG 1 cut(s) 653
Eco31I GGTCTC 2 cut(s) 62, 897
Eco32I GATATC 1 cut(s) 117
Eco47I GGWCC 2 cut(s) 414, 933
Eco91I GGTNACC 1 cut(s) 824
EcoO65I GGTNACC 1 cut(s) 824
EcoRI GAATTC 2 cut(s) 679, 697
EcoRII CCWGG 1 cut(s) 929
EcoRV GATATC 1 cut(s) 117
EcoT14I CCWWGG 1 cut(s) 653
EcoT22I ATGCAT 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 653
FaeI CATG 4 cut(s) 70, 259, 629, 644
FaqI GGGAC 1 cut(s) 603
FatI CATG 4 cut(s) 66, 255, 625, 640
FauI CCCGC 2 cut(s) 416, 970
Fnu4HI GCNGC 3 cut(s) 45, 298, 745
FokI GGATG 5 cut(s) 174, 253, 304, 385, 670
Fsp4HI GCNGC 3 cut(s) 45, 298, 745
FspBI CTAG 1 cut(s) 654
GluI GCNGC 3 cut(s) 45, 298, 745
GsuI CTGGAG 1 cut(s) 913
HaeIII GGCC 1 cut(s) 166
HapII CCGG 5 cut(s) 330, 385, 412, 829, 1029
Hin1II CATG 4 cut(s) 70, 259, 629, 644
HinfI GANTC 6 cut(s) 4, 33, 314, 333, 344, 981
HpaII CCGG 5 cut(s) 330, 385, 412, 829, 1029
HphI GGTGA 4 cut(s) 385, 412, 616, 656
Hpy188I TCNGA 5 cut(s) 670, 689, 902, 937, 947
Hpy188III TCNNGA 5 cut(s) 67, 191, 330, 337, 437
HpyAV CCTTC 3 cut(s) 261, 373, 932
HpyCH4III ACNGT 4 cut(s) 267, 294, 418, 998
HpyCH4IV ACGT 3 cut(s) 913, 968, 1007
HpyCH4V TGCA 6 cut(s) 278, 297, 306, 497, 541, 1052
HpyF10VI GCNNNNNNNGC 1 cut(s) 303
HpyF3I CTNAG 2 cut(s) 374, 510
HpySE526I ACGT 3 cut(s) 913, 968, 1007
Hsp92II CATG 4 cut(s) 70, 259, 629, 644
Kpn2I TCCGGA 1 cut(s) 329
Kzo9I GATC 6 cut(s) 126, 130, 433, 598, 850, 942
LmnI GCTCC 2 cut(s) 332, 947
Lsp1109I GCAGC 2 cut(s) 31, 309
LweI GCATC 3 cut(s) 265, 407, 571
MaeI CTAG 1 cut(s) 654
MaeII ACGT 3 cut(s) 913, 968, 1007
MaeIII GTNAC 3 cut(s) 418, 824, 1055
MalI GATC 6 cut(s) 128, 132, 435, 600, 852, 944
MboI GATC 6 cut(s) 126, 130, 433, 598, 850, 942
MboII GAAGA 9 cut(s) 108, 155, 182, 199, 244, 247, 688, 1016, 1121
MfeI CAATTG 2 cut(s) 307, 557
MflI RGATCY 1 cut(s) 598
MhlI GDGCHC 1 cut(s) 924
MluCI AATT 8 cut(s) 173, 307, 456, 557, 679, 697, 1064, 1112
MmeI TCCRAC 1 cut(s) 345
MnlI CCTC 6 cut(s) 156, 221, 231, 390, 582, 885
Mph1103I ATGCAT 1 cut(s) 280
MroI TCCGGA 1 cut(s) 329
MroXI GAANNNNTTC 1 cut(s) 183
MseI TTAA 3 cut(s) 882, 971, 1123
MslI CAYNNNNRTG 3 cut(s) 65, 292, 579
MspI CCGG 5 cut(s) 330, 385, 412, 829, 1029
MspR9I CCNGG 2 cut(s) 931, 1029
MunI CAATTG 2 cut(s) 307, 557
MvaI CCWGG 1 cut(s) 931
MwoI GCNNNNNNNGC 1 cut(s) 303
NciI CCSGG 1 cut(s) 1029
NdeII GATC 6 cut(s) 126, 130, 433, 598, 850, 942
NlaIII CATG 4 cut(s) 70, 259, 629, 644
NlaIV GGNNCC 1 cut(s) 949
NmuCI GTSAC 2 cut(s) 418, 1055
NsiI ATGCAT 1 cut(s) 280
NspI RCATGY 1 cut(s) 629
NspV TTCGAA 1 cut(s) 876
PagI TCATGA 1 cut(s) 66
PcsI WCGNNNNNNNCGW 1 cut(s) 844
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 6 cut(s) 4, 33, 314, 333, 344, 981
Pfl23II CGTACG 1 cut(s) 998
PkrI GCNGC 3 cut(s) 46, 299, 746
Ppu21I YACGTR 1 cut(s) 1008
Psp6I CCWGG 1 cut(s) 929
PspEI GGTNACC 1 cut(s) 824
PspGI CCWGG 1 cut(s) 929
PspLI CGTACG 1 cut(s) 998
PspN4I GGNNCC 1 cut(s) 949
PspPI GGNCC 2 cut(s) 414, 933
PstNI CAGNNNCTG 1 cut(s) 303
PsuI RGATCY 1 cut(s) 598
RsaI GTAC 4 cut(s) 16, 450, 1000, 1119
RsaNI GTAC 4 cut(s) 15, 449, 999, 1118
RseI CAYNNNNRTG 3 cut(s) 65, 292, 579
Rsr2I CGGWCCG 1 cut(s) 414
RsrII CGGWCCG 1 cut(s) 414
SaqAI TTAA 3 cut(s) 882, 971, 1123
SatI GCNGC 3 cut(s) 45, 298, 745
Sau3AI GATC 6 cut(s) 126, 130, 433, 598, 850, 942
Sau96I GGNCC 2 cut(s) 414, 933
ScaI AGTACT 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 931, 1029
SduI GDGCHC 1 cut(s) 924
SfaNI GCATC 3 cut(s) 265, 407, 571
SfcI CTRYAG 1 cut(s) 48
SfuI TTCGAA 1 cut(s) 876
SinI GGWCC 2 cut(s) 414, 933
SmiMI CAYNNNNRTG 3 cut(s) 65, 292, 579
Sse9I AATT 8 cut(s) 173, 307, 456, 557, 679, 697, 1064, 1112
SsiI CCGC 5 cut(s) 396, 409, 423, 745, 977
SspMI CTAG 1 cut(s) 654
StyD4I CCNGG 2 cut(s) 929, 1027
StyI CCWWGG 1 cut(s) 653
TaaI ACNGT 4 cut(s) 267, 294, 418, 998
TaiI ACGT 3 cut(s) 916, 971, 1010
TaqI TCGA 7 cut(s) 119, 129, 213, 262, 336, 876, 984
TaqII GACCGA 1 cut(s) 273
TasI AATT 8 cut(s) 173, 307, 456, 557, 679, 697, 1064, 1112
TatI WGTACW 2 cut(s) 14, 448
TauI GCSGC 1 cut(s) 747
TfiI GAWTC 6 cut(s) 4, 33, 314, 333, 344, 981
Tru1I TTAA 3 cut(s) 882, 971, 1123
Tru9I TTAA 3 cut(s) 882, 971, 1123
TscAI CASTG 3 cut(s) 233, 272, 967
TseFI GTSAC 2 cut(s) 418, 1055
TseI GCWGC 2 cut(s) 44, 297
Tsp45I GTSAC 2 cut(s) 418, 1055
TspDTI ATGAA 7 cut(s) 17, 83, 176, 306, 542, 629, 1005
TspGWI ACGGA 1 cut(s) 166
TspRI CASTG 3 cut(s) 233, 272, 967
VpaK11BI GGWCC 2 cut(s) 414, 933
XapI RAATTY 4 cut(s) 173, 679, 697, 1064
XceI RCATGY 1 cut(s) 629
XcmI CCANNNNNNNNNTGG 1 cut(s) 592
XmaJI CCTAGG 1 cut(s) 653
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 1 cut(s) 654
ZrmI AGTACT 1 cut(s) 16
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.