Rh4BG328800

Cleavage and polyadenylation specificity factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0013739
Physical Location & Seq
Forward (+)
49472981 .. 49473607
627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG328800.1

Sequence Viewer

Length: 408 bp
ATGTCTGGTAAACCGACCAAAATTGATCTGGACAAGGACACCCGAATTGATGTGCGATGCCAGATTCATCAATTGACTTTCAGTCCTCATACAGATGCCAAAGGGATTATGGATCTGGTGAAGTTTCTTTCTCCCAAGCACGTGATACTTGTTCATGGTGAGAAGCCTAGGATGGCTACTCTGAAAGGAAGAATTCAATCCGAACTGGGAATTCAAAGTTGCTCAAATCCAAACTTCAAGTTTTCTACAGGCGGCTCAGAACAAGAACAAATCCAAACTCCGATGCATTTTTTTTTTTCGGGATGTCCCTGTCCCGGCCCGGTCCCGGCCCGGTACCGTTCGGGTTCGGGCCCGTCGGGATCACTTCTCAAAATAGCATTAACCGTCCCGTCCCGTCCCGAAAAATAA

Protein Analysis

135

Amino Acids

14.8

Weight (kDa)

9.51

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RMMBL PF07521 15 - 72 4.6e-16 Zn-dependent metallo-hydrolase RNA specificity domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 333
AccB1I GGYRCC 1 cut(s) 333
AciI CCGC 1 cut(s) 252
AclWI GGATC 2 cut(s) 120, 367
AcsI RAATTY 2 cut(s) 192, 210
AcvI CACGTG 1 cut(s) 142
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 2 cut(s) 314, 325
AgsI TTSAA 3 cut(s) 197, 215, 238
AhdI GACNNNNNGTC 1 cut(s) 81
AlwI GGATC 2 cut(s) 120, 367
AoxI GGCC 3 cut(s) 316, 327, 349
ApaI GGGCCC 1 cut(s) 353
ApoI RAATTY 2 cut(s) 192, 210
Asp718I GGTACC 1 cut(s) 333
AspA2I CCTAGG 1 cut(s) 167
AspS9I GGNCC 5 cut(s) 317, 322, 328, 349, 350
AsuC2I CCSGG 4 cut(s) 315, 320, 326, 331
AsuHPI GGTGA 2 cut(s) 130, 170
AvaII GGWCC 1 cut(s) 322
AvrII CCTAGG 1 cut(s) 167
BaeGI GKGCMC 1 cut(s) 353
BanI GGYRCC 1 cut(s) 333
BanII GRGCYC 1 cut(s) 353
BbrPI CACGTG 1 cut(s) 142
BccI CCATC 1 cut(s) 166
BcnI CCSGG 4 cut(s) 315, 320, 326, 331
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 1 cut(s) 246
BisI GCNGC 1 cut(s) 253
BlnI CCTAGG 1 cut(s) 167
BlsI GCNGC 1 cut(s) 254
Bme1390I CCNGG 4 cut(s) 315, 320, 326, 331
Bme18I GGWCC 1 cut(s) 322
BmeRI GACNNNNNGTC 1 cut(s) 81
BmgT120I GGNCC 5 cut(s) 317, 322, 328, 349, 350
BmiI GGNNCC 3 cut(s) 324, 335, 351
BmrFI CCNGG 4 cut(s) 315, 320, 326, 331
BmrI ACTGGG 1 cut(s) 215
BmsI GCATC 3 cut(s) 47, 85, 273
BmuI ACTGGG 1 cut(s) 215
BpuMI CCSGG 4 cut(s) 315, 320, 326, 331
BsaAI YACGTR 1 cut(s) 142
BsaJI CCNNGG 1 cut(s) 167
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 325
Bse1I ACTGG 1 cut(s) 210
BseDI CCNNGG 1 cut(s) 167
BseGI GGATG 2 cut(s) 177, 308
BseLI CCNNNNNNNGG 2 cut(s) 314, 325
BseMII CTCAG 1 cut(s) 270
BseNI ACTGG 1 cut(s) 210
BseSI GKGCMC 1 cut(s) 353
BshFI GGCC 3 cut(s) 318, 329, 351
BshNI GGYRCC 1 cut(s) 333
BsiSI CCGG 4 cut(s) 315, 320, 326, 331
BslFI GGGAC 6 cut(s) 291, 297, 308, 371, 376, 381
BslI CCNNNNNNNGG 2 cut(s) 314, 325
BsmFI GGGAC 6 cut(s) 291, 297, 308, 371, 376, 381
BsnI GGCC 3 cut(s) 318, 329, 351
Bsp120I GGGCCC 1 cut(s) 349
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 3 cut(s) 25, 112, 359
BspACI CCGC 1 cut(s) 252
BspANI GGCC 3 cut(s) 318, 329, 351
BspCNI CTCAG 1 cut(s) 269
BspLI GGNNCC 3 cut(s) 324, 335, 351
BspPI GGATC 2 cut(s) 120, 367
BspT107I GGYRCC 1 cut(s) 333
BsrI ACTGG 1 cut(s) 210
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 3 cut(s) 25, 112, 359
BssT1I CCWWGG 1 cut(s) 167
Bst4CI ACNGT 2 cut(s) 338, 385
BstBAI YACGTR 1 cut(s) 142
BstDEI CTNAG 1 cut(s) 256
BstF5I GGATG 2 cut(s) 177, 308
BstKTI GATC 3 cut(s) 28, 115, 362
BstMBI GATC 3 cut(s) 25, 112, 359
BstSCI CCNGG 4 cut(s) 313, 318, 324, 329
BstSFI CTRYAG 1 cut(s) 246
BstSLI GKGCMC 1 cut(s) 353
BstX2I RGATCY 1 cut(s) 112
BstYI RGATCY 1 cut(s) 112
BsuRI GGCC 3 cut(s) 318, 329, 351
BtgZI GCGATG 1 cut(s) 70
BtsCI GGATG 2 cut(s) 177, 308
Cfr13I GGNCC 5 cut(s) 317, 322, 328, 349, 350
Csp6I GTAC 1 cut(s) 334
CviAII CATG 1 cut(s) 155
CviJI RGCY 6 cut(s) 166, 176, 255, 318, 329, 351
CviKI_1 RGCY 6 cut(s) 166, 176, 255, 318, 329, 351
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 1 cut(s) 256
DpnI GATC 3 cut(s) 27, 114, 361
DpnII GATC 3 cut(s) 25, 112, 359
DriI GACNNNNNGTC 1 cut(s) 81
Eam1105I GACNNNNNGTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 167
Eco24I GRGCYC 1 cut(s) 353
Eco47I GGWCC 1 cut(s) 322
Eco72I CACGTG 1 cut(s) 142
EcoRI GAATTC 2 cut(s) 192, 210
EcoT14I CCWWGG 1 cut(s) 167
EcoT22I ATGCAT 1 cut(s) 288
EcoT38I GRGCYC 1 cut(s) 353
ErhI CCWWGG 1 cut(s) 167
FaeI CATG 1 cut(s) 158
FaiI YATR 3 cut(s) 90, 110, 156
FaqI GGGAC 6 cut(s) 291, 297, 308, 371, 376, 381
FatI CATG 1 cut(s) 154
Fnu4HI GCNGC 1 cut(s) 253
FokI GGATG 2 cut(s) 184, 315
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 1 cut(s) 253
FspBI CTAG 1 cut(s) 168
GluI GCNGC 1 cut(s) 253
HaeIII GGCC 3 cut(s) 318, 329, 351
HapII CCGG 4 cut(s) 315, 320, 326, 331
Hin1II CATG 1 cut(s) 158
HinfI GANTC 1 cut(s) 64
HpaII CCGG 4 cut(s) 315, 320, 326, 331
HphI GGTGA 2 cut(s) 130, 170
Hpy166II GTNNAC 1 cut(s) 11
Hpy188I TCNGA 4 cut(s) 183, 202, 259, 282
Hpy188III TCNNGA 4 cut(s) 29, 300, 357, 398
Hpy8I GTNNAC 1 cut(s) 11
Hpy99I CGWCG 1 cut(s) 358
HpyCH4III ACNGT 2 cut(s) 338, 385
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 1 cut(s) 286
HpyF3I CTNAG 1 cut(s) 256
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 1 cut(s) 158
KpnI GGTACC 1 cut(s) 337
Kzo9I GATC 3 cut(s) 25, 112, 359
LweI GCATC 3 cut(s) 47, 85, 273
MaeI CTAG 1 cut(s) 168
MaeII ACGT 1 cut(s) 141
MalI GATC 3 cut(s) 27, 114, 361
MboI GATC 3 cut(s) 25, 112, 359
MboII GAAGA 1 cut(s) 201
MfeI CAATTG 1 cut(s) 71
MflI RGATCY 1 cut(s) 112
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 5 cut(s) 21, 45, 71, 192, 210
MnlI CCTC 1 cut(s) 96
Mph1103I ATGCAT 1 cut(s) 288
MseI TTAA 1 cut(s) 380
MslI CAYNNNNRTG 1 cut(s) 93
MspI CCGG 4 cut(s) 315, 320, 326, 331
MspR9I CCNGG 4 cut(s) 315, 320, 326, 331
MunI CAATTG 1 cut(s) 71
NciI CCSGG 4 cut(s) 315, 320, 326, 331
NdeII GATC 3 cut(s) 25, 112, 359
NlaIII CATG 1 cut(s) 158
NlaIV GGNNCC 3 cut(s) 324, 335, 351
NsiI ATGCAT 1 cut(s) 288
PfeI GAWTC 1 cut(s) 64
PkrI GCNGC 1 cut(s) 254
PmaCI CACGTG 1 cut(s) 142
PmlI CACGTG 1 cut(s) 142
Ppu21I YACGTR 1 cut(s) 142
PspCI CACGTG 1 cut(s) 142
PspN4I GGNNCC 3 cut(s) 324, 335, 351
PspOMI GGGCCC 1 cut(s) 349
PspPI GGNCC 5 cut(s) 317, 322, 328, 349, 350
PsuI RGATCY 1 cut(s) 112
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
RseI CAYNNNNRTG 1 cut(s) 93
SaqAI TTAA 1 cut(s) 380
SatI GCNGC 1 cut(s) 253
Sau3AI GATC 3 cut(s) 25, 112, 359
Sau96I GGNCC 5 cut(s) 317, 322, 328, 349, 350
ScrFI CCNGG 4 cut(s) 315, 320, 326, 331
SduI GDGCHC 1 cut(s) 353
SetI ASST 1 cut(s) 144
SfaNI GCATC 3 cut(s) 47, 85, 273
SfcI CTRYAG 1 cut(s) 246
SinI GGWCC 1 cut(s) 322
SmiMI CAYNNNNRTG 1 cut(s) 93
Sse9I AATT 5 cut(s) 21, 45, 71, 192, 210
SsiI CCGC 1 cut(s) 252
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 4 cut(s) 313, 318, 324, 329
StyI CCWWGG 1 cut(s) 167
TaaI ACNGT 2 cut(s) 338, 385
TaiI ACGT 1 cut(s) 144
TasI AATT 5 cut(s) 21, 45, 71, 192, 210
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 1 cut(s) 64
Tru1I TTAA 1 cut(s) 380
Tru9I TTAA 1 cut(s) 380
TspDTI ATGAA 2 cut(s) 56, 143
VpaK11BI GGWCC 1 cut(s) 322
XapI RAATTY 2 cut(s) 192, 210
XcmI CCANNNNNNNNNTGG 2 cut(s) 25, 106
XmaJI CCTAGG 1 cut(s) 167
XspI CTAG 1 cut(s) 168
Zsp2I ATGCAT 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.