Rroxscaffold_7G00162220

Prolyl oligopeptidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013568
Physical Location & Seq
Forward (+)
4831905 .. 4835323
3419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00162220.1

Sequence Viewer

Length: 1311 bp
ATGGAACAACACTCGTTTTGTAATTATTATTTCCACAAAACGACAATAATACCTCTTTTAGTTTCCATTATAACGCTAACACAAACCCAACTCTTCTCTCCAATGAGCACAATGACCCACGGTCTCATCATCCATCTTCCTCTGCAGCACTCCACTCTATCATCACCATCCACCACCCTCCCGCACGTGCGATCTCCCCTGGCCCACACGTGGGCACGCCAAGGTCCCTCCCCGCCAAAAATATTCCCCTATAAATACCGGTCCCTGTCTCAGTCGACCAGACCCAGATCAAACACTGACACCCTAGGAACCCAAATGGCCGAGGAAGCTGTAGACGCCCAGAAGCTTCGTTCCGAGTTTTACGGAGTCTTGAGTAGCAGGCGATGTGGCGAAGTTCCGTTAACGGTGGAGCCGGCGAAACCGGTGACCAATCCTTTGTTTCAGGAGGCTAACCCTCCAAGCTTCAGCGAGGCAATGAACGCTTGCCCCAAGGCTGACATTACCAACTTTAAGGAGAAGCTTCAAGAGGAAAATCTTTACCTAATTACTGAGGCAGGAGAGCAGGGGAGGTTACCTGTGTTGATTTTGAGCATGCAGGAAGAAAAAAGTACCCAGAAAAAGCCTGCTGTTGTTTTTCTGCATAGTACTAACAAGAACAAAGAGTGGTTGAGACCATTGCTTGAGGCCTATGCTTCAAGGGGATATGTAGCGATTGCGATTGATTCTCGCTACCATGGTGAACGTGCCACTGATATAACCACTTACCAAGATGCTCTCATATCAGCATGGAAAAGAGGAAATACAATGCCATTCTTGTTTGATACGGTCTGGGACTTGATAAAATTGGCAGATTATCTAACACAGAGGGAGGATGTAGATCCTGCTAGGCTAGGAATCACTGGTGAATCACTGGGAGGTATGCATGCATGGTTGGCTGCTGCTGCTGACACCCGCTATGCAGTGGTTGTCCCTATAATTGGTGTTCAGGGGTTTCGATGGGCGATAGACAATGATAAGTGGCAGGCTCGAGTCGACAGTATAAAACCTGTTTTTGAAGAAGCACGAATTGATCTAAACAAGAGTGCGATTGACAAAGAAGTAGTGGACAAGGTGTGGGATAGGATTGCTCCAGGTCTGGCCTCTCAGTTTGATTCTCCTTATACAGTGCCAGCTATTGCATCGCGTCCTCTATTGATTTTAAATGGTGTAGAAGATCCTCGATGTCCACTCGCTGGTCTGGATGTTCCCAAAGCGAGAGCACTCAAGGCTTATGAAGATGCACAGCGTGTAGATAAATTTAAGATTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AXE1 Abhydrolase_6 Peptidase_S9

Protein Analysis

436

Amino Acids

48.88

Weight (kDa)

7.15

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AXE1 PF05448 158 - 325 2.3e-07 Acetyl xylan esterase (AXE1)
Abhydrolase_6 PF12697 210 - 421 6.2e-07 Alpha/beta hydrolase family
Peptidase_S9 PF00326 230 - 332 2.4e-08 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013568)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25770 AT5G25770 AT5G25770
fragaria_vesca FvH4_2g35900
malus_domestica MD08G1116700.v1.1
prunus_persica Prupe.1G451100_v2.0.a1 Prupe.1G451100_v2.0.a1
pyrus_communis pycom08g09720
rosa_chinensis RchiOBHm_Chr6g0305921
rosa_laevigata RLG00000010846
rosa_multiflora Rmu_sc0001809.1_g000049
rosa_roxburghii Rroxscaffold_7G00162220
rosa_rugosa Rorug06G0345400
rosa_samantha Rh6AG456900 Rh6BG444400 Rh6CG470800 Rh6DG457500
rosa_wichuraiana Rw6G039860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 71
AccB7I CCANNNNNTGG 1 cut(s) 1232
AccI GTMKAC 3 cut(s) 275, 333, 1032
AccII CGCG 1 cut(s) 1183
AciI CCGC 3 cut(s) 182, 233, 952
AclWI GGATC 2 cut(s) 872, 1208
AcoI YGGCCR 1 cut(s) 318
AcsI RAATTY 1 cut(s) 1295
AcuI CTGAAG 1 cut(s) 448
AcvI CACGTG 2 cut(s) 187, 210
AcyI GRCGYC 1 cut(s) 336
AdeI CACNNNGTG 1 cut(s) 1286
AfaI GTAC 2 cut(s) 610, 646
AfiI CCNNNNNNNGG 4 cut(s) 210, 211, 977, 1232
AflIII ACRYGT 1 cut(s) 207
AgeI ACCGGT 2 cut(s) 258, 421
AgsI TTSAA 3 cut(s) 524, 696, 1055
AhdI GACNNNNNGTC 1 cut(s) 120
AjnI CCWGG 2 cut(s) 198, 1129
AluBI AGCT 5 cut(s) 329, 346, 462, 520, 1172
AluI AGCT 5 cut(s) 329, 346, 462, 520, 1172
Alw21I GWGCWC 2 cut(s) 110, 1261
Alw26I GTCTC 3 cut(s) 128, 273, 664
AlwI GGATC 2 cut(s) 872, 1208
Ama87I CYCGRG 1 cut(s) 1026
AoxI GGCC 4 cut(s) 201, 318, 684, 1137
ApeKI GCWGC 4 cut(s) 145, 935, 938, 941
ApoI RAATTY 1 cut(s) 1295
ArsI GACNNNNNNTTYG 2 cut(s) 418, 450
AseI ATTAAT 1 cut(s) 1305
AsiGI ACCGGT 2 cut(s) 258, 421
AspA2I CCTAGG 1 cut(s) 304
AspS9I GGNCC 3 cut(s) 202, 224, 261
AsuHPI GGTGA 4 cut(s) 156, 436, 749, 914
AvaI CYCGRG 1 cut(s) 1026
AvaII GGWCC 2 cut(s) 224, 261
AvrII CCTAGG 1 cut(s) 304
BaeGI GKGCMC 1 cut(s) 217
BbrPI CACGTG 2 cut(s) 187, 210
Bbv12I GWGCWC 2 cut(s) 110, 1261
BbvI GCAGC 4 cut(s) 157, 922, 925, 928
BccI CCATC 3 cut(s) 141, 175, 990
BciT130I CCWGG 2 cut(s) 200, 1131
BcoDI GTCTC 3 cut(s) 128, 273, 664
BfaI CTAG 3 cut(s) 305, 885, 890
BfmI CTRYAG 2 cut(s) 143, 330
BisI GCNGC 4 cut(s) 146, 936, 939, 942
BlnI CCTAGG 1 cut(s) 304
BlsI GCNGC 4 cut(s) 147, 937, 940, 943
BmcAI AGTACT 1 cut(s) 646
Bme1390I CCNGG 2 cut(s) 200, 1131
Bme18I GGWCC 2 cut(s) 224, 261
BmeRI GACNNNNNGTC 1 cut(s) 120
BmeT110I CYCGRG 1 cut(s) 1026
BmgT120I GGNCC 3 cut(s) 202, 224, 261
BmiI GGNNCC 4 cut(s) 226, 263, 310, 411
BmrFI CCNGG 2 cut(s) 200, 1131
BmrI ACTGGG 1 cut(s) 920
BmsI GCATC 3 cut(s) 760, 1187, 1267
BmuI ACTGGG 1 cut(s) 920
BpmI CTGGAG 1 cut(s) 1113
BpuEI CTTGAG 3 cut(s) 391, 701, 1247
BsaAI YACGTR 2 cut(s) 187, 210
BsaBI GATNNNNATC 1 cut(s) 876
BsaHI GRCGYC 1 cut(s) 336
BsaI GGTCTC 2 cut(s) 128, 664
BsaJI CCNNGG 7 cut(s) 118, 198, 220, 304, 321, 489, 733
BsaWI WCCGGW 2 cut(s) 258, 421
Bsc4I CCNNNNNNNGG 4 cut(s) 210, 211, 977, 1232
Bse118I RCCGGY 3 cut(s) 258, 412, 421
Bse1I ACTGG 2 cut(s) 904, 915
Bse3DI GCAATG 2 cut(s) 480, 674
Bse8I GATNNNNATC 1 cut(s) 876
BseBI CCWGG 2 cut(s) 200, 1131
BseDI CCNNGG 7 cut(s) 118, 198, 220, 304, 321, 489, 733
BseGI GGATG 4 cut(s) 129, 167, 877, 1246
BseJI GATNNNNATC 1 cut(s) 876
BseLI CCNNNNNNNGG 4 cut(s) 210, 211, 977, 1232
BseMI GCAATG 2 cut(s) 480, 674
BseMII CTCAG 3 cut(s) 284, 540, 1157
BseNI ACTGG 2 cut(s) 904, 915
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 4 cut(s) 157, 922, 925, 928
Bsh1236I CGCG 1 cut(s) 1183
BshFI GGCC 4 cut(s) 203, 320, 686, 1139
BshTI ACCGGT 2 cut(s) 258, 421
BsiHKAI GWGCWC 2 cut(s) 110, 1261
BsiHKCI CYCGRG 1 cut(s) 1026
BsiSI CCGG 3 cut(s) 259, 413, 422
BslFI GGGAC 4 cut(s) 210, 247, 845, 953
BslI CCNNNNNNNGG 4 cut(s) 210, 211, 977, 1232
BsmAI GTCTC 3 cut(s) 128, 273, 664
BsmFI GGGAC 4 cut(s) 210, 247, 845, 953
BsnI GGCC 4 cut(s) 203, 320, 686, 1139
Bso31I GGTCTC 2 cut(s) 128, 664
BsoBI CYCGRG 1 cut(s) 1026
Bsp1286I GDGCHC 3 cut(s) 110, 217, 1261
Bsp143I GATC 5 cut(s) 191, 287, 877, 1069, 1213
Bsp19I CCATGG 1 cut(s) 733
BspACI CCGC 3 cut(s) 182, 233, 952
BspANI GGCC 4 cut(s) 203, 320, 686, 1139
BspCNI CTCAG 3 cut(s) 283, 541, 1156
BspFNI CGCG 1 cut(s) 1183
BspLI GGNNCC 4 cut(s) 226, 263, 310, 411
BspMAI CTGCAG 1 cut(s) 147
BspPI GGATC 2 cut(s) 872, 1208
BspTNI GGTCTC 2 cut(s) 128, 664
BsrDI GCAATG 2 cut(s) 480, 674
BsrFI RCCGGY 3 cut(s) 258, 412, 421
BsrI ACTGG 2 cut(s) 904, 915
BssAI RCCGGY 3 cut(s) 258, 412, 421
BssECI CCNNGG 7 cut(s) 118, 198, 220, 304, 321, 489, 733
BssMI GATC 5 cut(s) 191, 287, 877, 1069, 1213
BssNI GRCGYC 1 cut(s) 336
BssT1I CCWWGG 4 cut(s) 220, 304, 489, 733
Bst2UI CCWGG 2 cut(s) 200, 1131
Bst4CI ACNGT 5 cut(s) 122, 406, 826, 1037, 1165
Bst6I CTCTTC 1 cut(s) 98
BstACI GRCGYC 1 cut(s) 336
BstBAI YACGTR 2 cut(s) 187, 210
BstC8I GCNNGC 9 cut(s) 217, 380, 414, 484, 593, 624, 924, 1023, 1170
BstDEI CTNAG 3 cut(s) 270, 549, 1143
BstDSI CCRYGG 2 cut(s) 118, 733
BstEII GGTNACC 2 cut(s) 424, 570
BstF5I GGATG 4 cut(s) 129, 167, 877, 1246
BstFNI CGCG 1 cut(s) 1183
BstKTI GATC 5 cut(s) 194, 290, 880, 1072, 1216
BstMAI GTCTC 3 cut(s) 128, 273, 664
BstMBI GATC 5 cut(s) 191, 287, 877, 1069, 1213
BstMWI GCNNNNNNNGC 6 cut(s) 326, 335, 479, 932, 941, 1265
BstNI CCWGG 2 cut(s) 200, 1131
BstNSI RCATGY 2 cut(s) 595, 926
BstPI GGTNACC 2 cut(s) 424, 570
BstSCI CCNGG 2 cut(s) 198, 1129
BstSFI CTRYAG 2 cut(s) 143, 330
BstSLI GKGCMC 1 cut(s) 217
BstUI CGCG 1 cut(s) 1183
BstV1I GCAGC 4 cut(s) 157, 922, 925, 928
BstX2I RGATCY 2 cut(s) 877, 1213
BstYI RGATCY 2 cut(s) 877, 1213
BsuRI GGCC 4 cut(s) 203, 320, 686, 1139
BtgI CCRYGG 2 cut(s) 118, 733
BtgZI GCGATG 2 cut(s) 397, 1164
BtsCI GGATG 4 cut(s) 129, 167, 877, 1246
BtsI GCAGTG 1 cut(s) 966
BtsIMutI CAGTG 6 cut(s) 294, 747, 897, 908, 966, 1170
Cac8I GCNNGC 9 cut(s) 217, 380, 414, 484, 593, 624, 924, 1023, 1170
Cfr10I RCCGGY 3 cut(s) 258, 412, 421
Cfr13I GGNCC 3 cut(s) 202, 224, 261
CseI GACGC 2 cut(s) 344, 1172
Csp6I GTAC 2 cut(s) 609, 645
CspAI ACCGGT 2 cut(s) 258, 421
CviAII CATG 5 cut(s) 592, 734, 786, 923, 927
CviQI GTAC 2 cut(s) 609, 645
DdeI CTNAG 3 cut(s) 270, 549, 1143
DpnI GATC 5 cut(s) 193, 289, 879, 1071, 1215
DpnII GATC 5 cut(s) 191, 287, 877, 1069, 1213
DraI TTTAAA 1 cut(s) 1200
DraIII CACNNNGTG 1 cut(s) 1286
DriI GACNNNNNGTC 1 cut(s) 120
EaeI YGGCCR 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 98
Eam1105I GACNNNNNGTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 98
Eco130I CCWWGG 4 cut(s) 220, 304, 489, 733
Eco147I AGGCCT 1 cut(s) 686
Eco31I GGTCTC 2 cut(s) 128, 664
Eco47I GGWCC 2 cut(s) 224, 261
Eco57I CTGAAG 1 cut(s) 448
Eco72I CACGTG 2 cut(s) 187, 210
Eco88I CYCGRG 1 cut(s) 1026
Eco91I GGTNACC 2 cut(s) 424, 570
EcoO109I RGGNCCY 1 cut(s) 224
EcoO65I GGTNACC 2 cut(s) 424, 570
EcoRII CCWGG 2 cut(s) 198, 1129
EcoT14I CCWWGG 4 cut(s) 220, 304, 489, 733
EcoT22I ATGCAT 2 cut(s) 924, 928
ErhI CCWWGG 4 cut(s) 220, 304, 489, 733
FaeI CATG 5 cut(s) 595, 737, 789, 926, 930
FaqI GGGAC 4 cut(s) 210, 247, 845, 953
FatI CATG 5 cut(s) 591, 733, 785, 922, 926
FauI CCCGC 3 cut(s) 189, 240, 959
FblI GTMKAC 3 cut(s) 275, 333, 1032
Fnu4HI GCNGC 4 cut(s) 146, 936, 939, 942
FokI GGATG 4 cut(s) 116, 154, 884, 1253
Fsp4HI GCNGC 4 cut(s) 146, 936, 939, 942
FspBI CTAG 3 cut(s) 305, 885, 890
GluI GCNGC 4 cut(s) 146, 936, 939, 942
GsuI CTGGAG 1 cut(s) 1113
HaeIII GGCC 4 cut(s) 203, 320, 686, 1139
HapII CCGG 3 cut(s) 259, 413, 422
HgaI GACGC 2 cut(s) 344, 1172
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 5 cut(s) 595, 737, 789, 926, 930
HincII GTYRAC 3 cut(s) 276, 402, 1033
HindII GTYRAC 3 cut(s) 276, 402, 1033
HindIII AAGCTT 3 cut(s) 344, 460, 518
HinfI GANTC 6 cut(s) 366, 722, 894, 905, 1029, 1151
HpaI GTTAAC 1 cut(s) 402
HpaII CCGG 3 cut(s) 259, 413, 422
HphI GGTGA 4 cut(s) 156, 436, 749, 914
Hpy166II GTNNAC 7 cut(s) 276, 334, 402, 740, 1033, 1105, 1226
Hpy188I TCNGA 1 cut(s) 355
Hpy188III TCNNGA 4 cut(s) 370, 443, 524, 1238
Hpy8I GTNNAC 7 cut(s) 276, 334, 402, 740, 1033, 1105, 1226
HpyCH4III ACNGT 5 cut(s) 122, 406, 826, 1037, 1165
HpyCH4IV ACGT 3 cut(s) 186, 209, 742
HpyCH4V TGCA 8 cut(s) 145, 595, 640, 922, 926, 959, 1178, 1280
HpyF10VI GCNNNNNNNGC 6 cut(s) 326, 335, 479, 932, 941, 1265
HpyF3I CTNAG 3 cut(s) 270, 549, 1143
HpySE526I ACGT 3 cut(s) 186, 209, 742
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 5 cut(s) 595, 737, 789, 926, 930
KroI GCCGGC 1 cut(s) 412
KroNI GCCGGC 1 cut(s) 414
KspAI GTTAAC 1 cut(s) 402
Kzo9I GATC 5 cut(s) 191, 287, 877, 1069, 1213
LmnI GCTCC 2 cut(s) 409, 1132
Lsp1109I GCAGC 4 cut(s) 157, 922, 925, 928
LweI GCATC 3 cut(s) 760, 1187, 1267
MaeI CTAG 3 cut(s) 305, 885, 890
MaeII ACGT 3 cut(s) 186, 209, 742
MaeIII GTNAC 2 cut(s) 424, 570
MalI GATC 5 cut(s) 193, 289, 879, 1071, 1215
MboI GATC 5 cut(s) 191, 287, 877, 1069, 1213
MboII GAAGA 6 cut(s) 85, 128, 611, 1067, 1223, 1286
MflI RGATCY 2 cut(s) 877, 1213
MhlI GDGCHC 3 cut(s) 110, 217, 1261
MluCI AATT 7 cut(s) 22, 543, 842, 975, 1065, 1295, 1306
MlyI GAGTC 2 cut(s) 375, 1038
Mph1103I ATGCAT 2 cut(s) 924, 928
MroNI GCCGGC 1 cut(s) 412
MseI TTAA 5 cut(s) 401, 510, 1199, 1299, 1305
MspI CCGG 3 cut(s) 259, 413, 422
MspR9I CCNGG 2 cut(s) 200, 1131
MvaI CCWGG 2 cut(s) 200, 1131
MvnI CGCG 1 cut(s) 1183
MwoI GCNNNNNNNGC 6 cut(s) 326, 335, 479, 932, 941, 1265
NaeI GCCGGC 1 cut(s) 414
NcoI CCATGG 1 cut(s) 733
NdeII GATC 5 cut(s) 191, 287, 877, 1069, 1213
NgoMIV GCCGGC 1 cut(s) 412
NlaIII CATG 5 cut(s) 595, 737, 789, 926, 930
NlaIV GGNNCC 4 cut(s) 226, 263, 310, 411
NmeAIII GCCGAG 1 cut(s) 346
NmuCI GTSAC 1 cut(s) 424
NsiI ATGCAT 2 cut(s) 924, 928
NspI RCATGY 2 cut(s) 595, 926
PaeI GCATGC 2 cut(s) 595, 926
PaeR7I CTCGAG 1 cut(s) 1026
PceI AGGCCT 1 cut(s) 686
PdiI GCCGGC 1 cut(s) 414
PfeI GAWTC 4 cut(s) 722, 894, 905, 1151
PflMI CCANNNNNTGG 1 cut(s) 1232
PinAI ACCGGT 2 cut(s) 258, 421
PkrI GCNGC 4 cut(s) 147, 937, 940, 943
PleI GAGTC 2 cut(s) 374, 1037
PmaCI CACGTG 2 cut(s) 187, 210
PmlI CACGTG 2 cut(s) 187, 210
PpsI GAGTC 2 cut(s) 374, 1037
Ppu21I YACGTR 2 cut(s) 187, 210
PpuMI RGGWCCY 1 cut(s) 224
PshBI ATTAAT 1 cut(s) 1305
PsiI TTATAA 1 cut(s) 71
Psp5II RGGWCCY 1 cut(s) 224
Psp6I CCWGG 2 cut(s) 198, 1129
PspCI CACGTG 2 cut(s) 187, 210
PspEI GGTNACC 2 cut(s) 424, 570
PspGI CCWGG 2 cut(s) 198, 1129
PspN4I GGNNCC 4 cut(s) 226, 263, 310, 411
PspPI GGNCC 3 cut(s) 202, 224, 261
PspPPI RGGWCCY 1 cut(s) 224
PspXI VCTCGAGB 1 cut(s) 1026
PstI CTGCAG 1 cut(s) 147
PsuI RGATCY 2 cut(s) 877, 1213
RsaI GTAC 2 cut(s) 610, 646
RsaNI GTAC 2 cut(s) 609, 645
SalI GTCGAC 2 cut(s) 274, 1031
SaqAI TTAA 5 cut(s) 401, 510, 1199, 1299, 1305
SatI GCNGC 4 cut(s) 146, 936, 939, 942
Sau3AI GATC 5 cut(s) 191, 287, 877, 1069, 1213
Sau96I GGNCC 3 cut(s) 202, 224, 261
ScaI AGTACT 1 cut(s) 646
SchI GAGTC 2 cut(s) 375, 1038
ScrFI CCNGG 2 cut(s) 200, 1131
SduI GDGCHC 3 cut(s) 110, 217, 1261
SfaNI GCATC 3 cut(s) 760, 1187, 1267
SfcI CTRYAG 2 cut(s) 143, 330
Sfr274I CTCGAG 1 cut(s) 1026
SinI GGWCC 2 cut(s) 224, 261
SlaI CTCGAG 1 cut(s) 1026
SmlI CTYRAG 4 cut(s) 370, 680, 1026, 1262
SmoI CTYRAG 4 cut(s) 370, 680, 1026, 1262
SphI GCATGC 2 cut(s) 595, 926
Sse9I AATT 7 cut(s) 22, 543, 842, 975, 1065, 1295, 1306
SseBI AGGCCT 1 cut(s) 686
SsiI CCGC 3 cut(s) 182, 233, 952
SspI AATATT 1 cut(s) 243
SspMI CTAG 3 cut(s) 305, 885, 890
StuI AGGCCT 1 cut(s) 686
StyD4I CCNGG 2 cut(s) 198, 1129
StyI CCWWGG 4 cut(s) 220, 304, 489, 733
TaaI ACNGT 5 cut(s) 122, 406, 826, 1037, 1165
TaiI ACGT 3 cut(s) 189, 212, 745
TaqI TCGA 5 cut(s) 275, 994, 1027, 1032, 1219
TasI AATT 7 cut(s) 22, 543, 842, 975, 1065, 1295, 1306
TatI WGTACW 1 cut(s) 644
TfiI GAWTC 4 cut(s) 722, 894, 905, 1151
Tru1I TTAA 5 cut(s) 401, 510, 1199, 1299, 1305
Tru9I TTAA 5 cut(s) 401, 510, 1199, 1299, 1305
TscAI CASTG 6 cut(s) 301, 754, 904, 915, 966, 1170
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 4 cut(s) 145, 935, 938, 941
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 2 cut(s) 491, 1287
TspGWI ACGGA 2 cut(s) 378, 387
TspRI CASTG 6 cut(s) 301, 754, 904, 915, 966, 1170
Van91I CCANNNNNTGG 1 cut(s) 1232
VpaK11BI GGWCC 2 cut(s) 224, 261
VspI ATTAAT 1 cut(s) 1305
XapI RAATTY 1 cut(s) 1295
XceI RCATGY 2 cut(s) 595, 926
XhoI CTCGAG 1 cut(s) 1026
XmaJI CCTAGG 1 cut(s) 304
XmiI GTMKAC 3 cut(s) 275, 333, 1032
XspI CTAG 3 cut(s) 305, 885, 890
ZrmI AGTACT 1 cut(s) 646
Zsp2I ATGCAT 2 cut(s) 924, 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.