Rroxscaffold_7G00183450

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0017987
Physical Location & Seq
Forward (+)
22525255 .. 22529288
4034 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00183450.1

Sequence Viewer

Length: 636 bp
ATGAGGCGAGTGAGAAGAAGAATTGTTGGTTCCCTCTACCGTGGTGAGGAAGAGGGCGCCCTTCTCCGGGCTGTACATTTGATAAAAGAAGAGAGGGCAGCTAGTGCAAATGAACTCATACGGTGCTGTTGTCAAAGAATAATCAACAGGCTTTTCAGCATCCTAAGGCAAAGGGAATGTCCGGATTATCTGAGCACAGATATTCAAAGCTTGACCTTTGCTTCTTCAAAATGCTTAGATATACCGGAGCTGCAGACTGTCAAAACAATTTTTGAGAAGAAATTCGGTACAGATTTTGTGGCTGTTGCTATAAATGTTCTACCTGGGTGTAATGTACATCAGGAGATGAGATCGTTGCTAGCTACTGAACCCCCCACCCTTGCAAAAAAGTTGGAAACATTGAAGAAAATAGCAAAGGAGAATAACATTGACTGGTCATATTCCAAAGAAACTCTTGAATATGAGCATTGGGATACAGAGACTGAGTTTGAAGATAGCAACGAATATTTATATCCGGCCCCAACTGTCGCTGCTCCTCATATACATCCTAAATTACCAGACTACGAGAACTTAAGTCGCTTCTTTCATGACCTGTTCACAGAGCAAAAGCGAGGGTCCATAGAGAAGCTAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

211

Amino Acids

24.48

Weight (kDa)

6.33

Isoelectric Point (pI)

51.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 3 - 144 1.9e-30 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017987)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g19470
rosa_laevigata RLG00000012664
rosa_multiflora Rmu_sc0002535.1_g000006
rosa_roxburghii Rroxscaffold_7G00183450 Rroxscaffold_7G00183460 Rroxscaffold_7G00183570
rosa_samantha Rh6BG283800 Rh6BG284300 Rh6BG284400
rosa_wichuraiana Rw0G000380 Rw6G024340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 56
AccIII TCCGGA 1 cut(s) 181
AcsI RAATTY 1 cut(s) 281
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 3 cut(s) 75, 289, 336
AfiI CCNNNNNNNGG 3 cut(s) 46, 66, 67
AflII CTTAAG 1 cut(s) 571
AgsI TTSAA 5 cut(s) 206, 228, 403, 458, 491
AjnI CCWGG 1 cut(s) 322
AluBI AGCT 5 cut(s) 101, 210, 250, 362, 628
AluI AGCT 5 cut(s) 101, 210, 250, 362, 628
Alw21I GWGCWC 1 cut(s) 197
Alw26I GTCTC 1 cut(s) 473
AlwNI CAGNNNCTG 1 cut(s) 482
Aor13HI TCCGGA 1 cut(s) 181
AoxI GGCC 1 cut(s) 516
ApeKI GCWGC 3 cut(s) 98, 250, 530
ApoI RAATTY 1 cut(s) 281
ArsI GACNNNNNNTTYG 2 cut(s) 163, 195
Asp700I GAANNNNTTC 1 cut(s) 281
AspLEI GCGC 1 cut(s) 59
AspS9I GGNCC 2 cut(s) 517, 615
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 1 cut(s) 56
AsuNHI GCTAGC 1 cut(s) 358
AvaII GGWCC 1 cut(s) 615
AxyI CCTNAGG 1 cut(s) 164
BanI GGYRCC 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 197
BbvI GCAGC 3 cut(s) 110, 237, 517
BciT130I CCWGG 1 cut(s) 324
BciVI GTATCC 1 cut(s) 466
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 1 cut(s) 473
BfaI CTAG 2 cut(s) 102, 359
BfmI CTRYAG 1 cut(s) 251
BfoI RGCGCY 1 cut(s) 60
BfrI CTTAAG 1 cut(s) 571
BfuI GTATCC 1 cut(s) 466
BisI GCNGC 3 cut(s) 99, 251, 531
BlsI GCNGC 3 cut(s) 100, 252, 532
Bme1390I CCNGG 2 cut(s) 68, 324
Bme18I GGWCC 1 cut(s) 615
BmgT120I GGNCC 2 cut(s) 517, 615
BmiI GGNNCC 4 cut(s) 31, 58, 519, 616
BmrFI CCNGG 2 cut(s) 68, 324
BmsI GCATC 1 cut(s) 168
BmtI GCTAGC 1 cut(s) 362
BpuMI CCSGG 1 cut(s) 68
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 2 cut(s) 40, 323
BsaWI WCCGGW 2 cut(s) 181, 244
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 66, 67
Bse1I ACTGG 1 cut(s) 437
Bse21I CCTNAGG 1 cut(s) 164
BseAI TCCGGA 1 cut(s) 181
BseBI CCWGG 1 cut(s) 324
BseDI CCNNGG 2 cut(s) 40, 323
BseGI GGATG 2 cut(s) 159, 544
BseLI CCNNNNNNNGG 3 cut(s) 46, 66, 67
BseMII CTCAG 2 cut(s) 182, 474
BseNI ACTGG 1 cut(s) 437
BseRI GAGGAG 1 cut(s) 525
BseXI GCAGC 3 cut(s) 110, 237, 517
BshFI GGCC 1 cut(s) 518
BshNI GGYRCC 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 197
BsiSI CCGG 4 cut(s) 67, 182, 245, 515
BslI CCNNNNNNNGG 3 cut(s) 46, 66, 67
BsmAI GTCTC 1 cut(s) 473
BsnI GGCC 1 cut(s) 518
Bsp1286I GDGCHC 1 cut(s) 197
Bsp13I TCCGGA 1 cut(s) 181
Bsp1407I TGTACA 2 cut(s) 73, 334
Bsp143I GATC 1 cut(s) 350
BspANI GGCC 1 cut(s) 518
BspCNI CTCAG 2 cut(s) 183, 475
BspEI TCCGGA 1 cut(s) 181
BspHI TCATGA 1 cut(s) 586
BspLI GGNNCC 4 cut(s) 31, 58, 519, 616
BspMAI CTGCAG 1 cut(s) 255
BspOI GCTAGC 1 cut(s) 362
BspT107I GGYRCC 1 cut(s) 56
BspTI CTTAAG 1 cut(s) 571
BsrGI TGTACA 2 cut(s) 73, 334
BsrI ACTGG 1 cut(s) 437
BssECI CCNNGG 2 cut(s) 40, 323
BssMI GATC 1 cut(s) 350
BssNI GRCGYC 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 4 cut(s) 41, 123, 259, 526
Bst6I CTCTTC 2 cut(s) 45, 84
BstACI GRCGYC 1 cut(s) 57
BstAFI CTTAAG 1 cut(s) 571
BstAPI GCANNNNNTGC 1 cut(s) 104
BstAUI TGTACA 2 cut(s) 73, 334
BstC8I GCNNGC 1 cut(s) 360
BstDEI CTNAG 4 cut(s) 164, 191, 235, 483
BstDSI CCRYGG 1 cut(s) 40
BstF5I GGATG 2 cut(s) 159, 544
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 1 cut(s) 353
BstMAI GTCTC 1 cut(s) 473
BstMBI GATC 1 cut(s) 350
BstMWI GCNNNNNNNGC 1 cut(s) 104
BstNI CCWGG 1 cut(s) 324
BstSCI CCNGG 2 cut(s) 66, 322
BstSFI CTRYAG 1 cut(s) 251
BstV1I GCAGC 3 cut(s) 110, 237, 517
Bsu36I CCTNAGG 1 cut(s) 164
BsuI GTATCC 1 cut(s) 466
BsuRI GGCC 1 cut(s) 518
BtgI CCRYGG 1 cut(s) 40
BtsCI GGATG 2 cut(s) 159, 544
Cac8I GCNNGC 1 cut(s) 360
CaiI CAGNNNCTG 1 cut(s) 482
CciI TCATGA 1 cut(s) 586
CfoI GCGC 1 cut(s) 59
Cfr13I GGNCC 2 cut(s) 517, 615
Csp6I GTAC 3 cut(s) 74, 288, 335
CviAII CATG 1 cut(s) 587
CviJI RGCY 9 cut(s) 71, 101, 151, 210, 250, 302, 362, 518, 628
CviKI_1 RGCY 9 cut(s) 71, 101, 151, 210, 250, 302, 362, 518, 628
CviQI GTAC 3 cut(s) 74, 288, 335
DdeI CTNAG 4 cut(s) 164, 191, 235, 483
DinI GGCGCC 1 cut(s) 58
DpnI GATC 1 cut(s) 352
DpnII GATC 1 cut(s) 350
Eam1104I CTCTTC 2 cut(s) 45, 84
EarI CTCTTC 2 cut(s) 45, 84
Eco47I GGWCC 1 cut(s) 615
Eco81I CCTNAGG 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 322
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
FaeI CATG 1 cut(s) 590
FalI AAGNNNNNCTT 2 cut(s) 438, 470
FatI CATG 1 cut(s) 586
Fnu4HI GCNGC 3 cut(s) 99, 251, 531
FokI GGATG 2 cut(s) 146, 531
Fsp4HI GCNGC 3 cut(s) 99, 251, 531
FspBI CTAG 2 cut(s) 102, 359
GlaI GCGC 1 cut(s) 58
GluI GCNGC 3 cut(s) 99, 251, 531
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 1 cut(s) 518
HapII CCGG 4 cut(s) 67, 182, 245, 515
HhaI GCGC 1 cut(s) 59
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 1 cut(s) 590
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HindIII AAGCTT 1 cut(s) 208
HpaII CCGG 4 cut(s) 67, 182, 245, 515
HphI GGTGA 1 cut(s) 56
Hpy166II GTNNAC 1 cut(s) 597
Hpy188I TCNGA 1 cut(s) 192
Hpy188III TCNNGA 4 cut(s) 182, 341, 455, 587
Hpy8I GTNNAC 1 cut(s) 597
HpyAV CCTTC 1 cut(s) 71
HpyCH4III ACNGT 4 cut(s) 41, 123, 259, 526
HpyCH4V TGCA 3 cut(s) 107, 253, 383
HpyF10VI GCNNNNNNNGC 1 cut(s) 104
HpyF3I CTNAG 4 cut(s) 164, 191, 235, 483
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 1 cut(s) 590
HspAI GCGC 1 cut(s) 57
KasI GGCGCC 1 cut(s) 56
Kpn2I TCCGGA 1 cut(s) 181
Kzo9I GATC 1 cut(s) 350
LmnI GCTCC 2 cut(s) 247, 538
Lsp1109I GCAGC 3 cut(s) 110, 237, 517
LweI GCATC 1 cut(s) 168
MaeI CTAG 2 cut(s) 102, 359
MalI GATC 1 cut(s) 352
MboI GATC 1 cut(s) 350
MboII GAAGA 8 cut(s) 27, 30, 62, 101, 216, 289, 415, 503
MhlI GDGCHC 1 cut(s) 197
MluCI AATT 4 cut(s) 21, 267, 281, 551
Mly113I GGCGCC 1 cut(s) 57
MmeI TCCRAC 1 cut(s) 372
MnlI CCTC 6 cut(s) 40, 44, 46, 87, 546, 605
MroI TCCGGA 1 cut(s) 181
MroXI GAANNNNTTC 1 cut(s) 281
MseI TTAA 1 cut(s) 572
MspCI CTTAAG 1 cut(s) 571
MspI CCGG 4 cut(s) 67, 182, 245, 515
MspR9I CCNGG 2 cut(s) 68, 324
MvaI CCWGG 1 cut(s) 324
MwoI GCNNNNNNNGC 1 cut(s) 104
NarI GGCGCC 1 cut(s) 57
NciI CCSGG 1 cut(s) 68
NdeII GATC 1 cut(s) 350
NheI GCTAGC 1 cut(s) 358
NlaIII CATG 1 cut(s) 590
NlaIV GGNNCC 4 cut(s) 31, 58, 519, 616
PagI TCATGA 1 cut(s) 586
PdmI GAANNNNTTC 1 cut(s) 281
PkrI GCNGC 3 cut(s) 100, 252, 532
PluTI GGCGCC 1 cut(s) 60
Psp6I CCWGG 1 cut(s) 322
PspGI CCWGG 1 cut(s) 322
PspN4I GGNNCC 4 cut(s) 31, 58, 519, 616
PspPI GGNCC 2 cut(s) 517, 615
PstI CTGCAG 1 cut(s) 255
PstNI CAGNNNCTG 1 cut(s) 482
RsaI GTAC 3 cut(s) 75, 289, 336
RsaNI GTAC 3 cut(s) 74, 288, 335
SaqAI TTAA 1 cut(s) 572
SatI GCNGC 3 cut(s) 99, 251, 531
Sau3AI GATC 1 cut(s) 350
Sau96I GGNCC 2 cut(s) 517, 615
ScrFI CCNGG 2 cut(s) 68, 324
SduI GDGCHC 1 cut(s) 197
SetI ASST 8 cut(s) 103, 212, 218, 252, 325, 364, 594, 630
SfaNI GCATC 1 cut(s) 168
SfcI CTRYAG 1 cut(s) 251
SfoI GGCGCC 1 cut(s) 58
SinI GGWCC 1 cut(s) 615
SmlI CTYRAG 1 cut(s) 571
SmoI CTYRAG 1 cut(s) 571
Sse9I AATT 4 cut(s) 21, 267, 281, 551
SspDI GGCGCC 1 cut(s) 56
SspI AATATT 1 cut(s) 506
SspMI CTAG 2 cut(s) 102, 359
StyD4I CCNGG 2 cut(s) 66, 322
TaaI ACNGT 4 cut(s) 41, 123, 259, 526
TasI AATT 4 cut(s) 21, 267, 281, 551
TatI WGTACW 2 cut(s) 73, 334
Tru1I TTAA 1 cut(s) 572
Tru9I TTAA 1 cut(s) 572
TseI GCWGC 3 cut(s) 98, 250, 530
TspDTI ATGAA 2 cut(s) 126, 575
Vha464I CTTAAG 1 cut(s) 571
VpaK11BI GGWCC 1 cut(s) 615
XapI RAATTY 1 cut(s) 281
XmnI GAANNNNTTC 1 cut(s) 281
XspI CTAG 2 cut(s) 102, 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.