Rh6BG283800

Regulator of Vps4 activity in the MVB pathway protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0017987
Physical Location & Seq
Forward (+)
50693386 .. 50700061
6676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG283800.1

Sequence Viewer

Length: 843 bp
ATGTTTCAGAGGTCCTGCGGATGCGTCCAGGACGAGTTCTCATTTGACGAATGGGTATCTGCATTAAATGACGTCCGGACTCAAACAAACGCTTTGAGGGAATCAAAACTGAAAACAATACAGCGCATTAGAAAAGAAATTGCCTGTTCCCTTCGTCAAGGTCAGTCACAAGAAGAGCAAGCTGTTCACCAGGCTGGGCAGATATTAAAAGAGATGGGGTCTTACAATGCACTTCAAATCATACGGCCATCTTGTGAAGGCATACTTATCAGGCTTATCTTCATCGCAAGGCAAAGGAAGTGTCCTGAAGATCTTAAAATGGAGATTCAAAACTTGAGCTTTGCTGCCTCAAGGTGTCTAGATATACCAGGGCTGAAGGCTCTCAAAGACATTTTCGAGAAGAGATTTGGGAAAAAATTTGTGGCTGAAGCTACAAATCTTCGAACCGGGTGTCATGTACAAAGAGAGCTGATAACAATGCTATCTACTGAACACTTACTGCAAAAAAAGTTGGAACTATTGGAGGAAATAGCCAAGGACAATGAAATTAATTGGAAATATTCTAAAGAGTCCGTTGAAGATCAGATCCGAAATTTTTTTGGTGATTCTGCTAAACGTGTGCAAGTAAACATTGAGCCTAATGGCGATGAAACTAGGTACTTTCACCAAACAACTCCATGTTCAGATGTCGAGTCTGTTTATCAAGATTGTGATGAATATCTCTATCCAGAGCCATCTCCTGCTGCTCCTCATACACACCCTAATTTGCCAGACTGTGATAATGTATGGATCTTTCATGCAGAACAGAAGAAACATTACCAACAACACAAACCAAATGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

280

Amino Acids

32.46

Weight (kDa)

6.17

Isoelectric Point (pI)

52.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 27 - 184 5.6e-27 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017987)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g19470
rosa_laevigata RLG00000012664
rosa_multiflora Rmu_sc0002535.1_g000006
rosa_roxburghii Rroxscaffold_7G00183450 Rroxscaffold_7G00183460 Rroxscaffold_7G00183570
rosa_samantha Rh6BG283800 Rh6BG284300 Rh6BG284400
rosa_wichuraiana Rw0G000380 Rw6G024340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 75
AccIII TCCGGA 1 cut(s) 75
AciI CCGC 1 cut(s) 18
AclWI GGATC 2 cut(s) 580, 797
AcoI YGGCCR 1 cut(s) 245
AcsI RAATTY 2 cut(s) 416, 592
AcuI CTGAAG 3 cut(s) 327, 395, 447
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 2 cut(s) 459, 659
AflIII ACRYGT 1 cut(s) 616
AgsI TTSAA 3 cut(s) 236, 329, 578
AjnI CCWGG 3 cut(s) 27, 189, 367
AluBI AGCT 4 cut(s) 182, 339, 431, 469
AluI AGCT 4 cut(s) 182, 339, 431, 469
AlwI GGATC 2 cut(s) 580, 797
Aor13HI TCCGGA 1 cut(s) 75
AoxI GGCC 1 cut(s) 245
ApeKI GCWGC 2 cut(s) 344, 743
ApoI RAATTY 2 cut(s) 416, 592
ArsI GACNNNNNNTTYG 5 cut(s) 286, 318, 436, 468, 822
AseI ATTAAT 1 cut(s) 549
AspLEI GCGC 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 12
AsuC2I CCSGG 1 cut(s) 448
AsuHPI GGTGA 3 cut(s) 179, 614, 656
AsuII TTCGAA 1 cut(s) 442
AvaII GGWCC 1 cut(s) 12
BarI GAAGNNNNNNTAC 2 cut(s) 800, 832
BbvI GCAGC 2 cut(s) 331, 730
BccI CCATC 3 cut(s) 208, 256, 742
BceAI ACGGC 1 cut(s) 260
BciT130I CCWGG 3 cut(s) 29, 191, 369
BcnI CCSGG 1 cut(s) 448
BfaI CTAG 3 cut(s) 359, 654, 841
BglII AGATCT 1 cut(s) 310
BisI GCNGC 2 cut(s) 345, 744
BlsI GCNGC 2 cut(s) 346, 745
Bme1390I CCNGG 4 cut(s) 29, 191, 369, 448
Bme18I GGWCC 1 cut(s) 12
BmgT120I GGNCC 1 cut(s) 12
BmrFI CCNGG 4 cut(s) 29, 191, 369, 448
BmsI GCATC 1 cut(s) 11
Bpu14I TTCGAA 1 cut(s) 442
BpuEI CTTGAG 2 cut(s) 334, 355
BpuMI CCSGG 1 cut(s) 448
BsaBI GATNNNNATC 1 cut(s) 717
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 2 cut(s) 368, 534
BsaWI WCCGGW 1 cut(s) 75
Bse8I GATNNNNATC 1 cut(s) 717
BseAI TCCGGA 1 cut(s) 75
BseBI CCWGG 3 cut(s) 29, 191, 369
BseDI CCNNGG 2 cut(s) 368, 534
BseGI GGATG 1 cut(s) 26
BseJI GATNNNNATC 1 cut(s) 717
BseRI GAGGAG 1 cut(s) 738
BseXI GCAGC 2 cut(s) 331, 730
BseYI CCCAGC 1 cut(s) 194
BshFI GGCC 1 cut(s) 247
BsiSI CCGG 2 cut(s) 76, 447
BsnI GGCC 1 cut(s) 247
Bsp119I TTCGAA 1 cut(s) 442
Bsp13I TCCGGA 1 cut(s) 75
Bsp1407I TGTACA 1 cut(s) 457
Bsp143I GATC 4 cut(s) 310, 580, 585, 789
BspACI CCGC 1 cut(s) 18
BspANI GGCC 1 cut(s) 247
BspEI TCCGGA 1 cut(s) 75
BspPI GGATC 2 cut(s) 580, 797
BspQI GCTCTTC 1 cut(s) 168
BspT104I TTCGAA 1 cut(s) 442
BsrGI TGTACA 1 cut(s) 457
BssECI CCNNGG 2 cut(s) 368, 534
BssMI GATC 4 cut(s) 310, 580, 585, 789
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 1 cut(s) 534
Bst2UI CCWGG 3 cut(s) 29, 191, 369
Bst4CI ACNGT 1 cut(s) 776
Bst6I CTCTTC 2 cut(s) 168, 395
BstACI GRCGYC 1 cut(s) 72
BstAUI TGTACA 1 cut(s) 457
BstBI TTCGAA 1 cut(s) 442
BstC8I GCNNGC 1 cut(s) 180
BstF5I GGATG 1 cut(s) 26
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 4 cut(s) 313, 583, 588, 792
BstMBI GATC 4 cut(s) 310, 580, 585, 789
BstNI CCWGG 3 cut(s) 29, 191, 369
BstSCI CCNGG 4 cut(s) 27, 189, 367, 446
BstV1I GCAGC 2 cut(s) 331, 730
BstX2I RGATCY 3 cut(s) 310, 585, 789
BstYI RGATCY 3 cut(s) 310, 585, 789
BsuRI GGCC 1 cut(s) 247
BtgZI GCGATG 2 cut(s) 268, 660
BtsCI GGATG 1 cut(s) 26
Cac8I GCNNGC 1 cut(s) 180
CfoI GCGC 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 12
CseI GACGC 1 cut(s) 13
Csp6I GTAC 2 cut(s) 458, 658
CviAII CATG 3 cut(s) 455, 678, 797
CviQI GTAC 2 cut(s) 458, 658
DpnI GATC 4 cut(s) 312, 582, 587, 791
DpnII GATC 4 cut(s) 310, 580, 585, 789
EaeI YGGCCR 1 cut(s) 245
Eam1104I CTCTTC 2 cut(s) 168, 395
EarI CTCTTC 2 cut(s) 168, 395
Eco130I CCWWGG 1 cut(s) 534
Eco47I GGWCC 1 cut(s) 12
Eco57I CTGAAG 3 cut(s) 327, 395, 447
EcoO109I RGGNCCY 1 cut(s) 12
EcoRII CCWGG 3 cut(s) 27, 189, 367
EcoT14I CCWWGG 1 cut(s) 534
ErhI CCWWGG 1 cut(s) 534
FaeI CATG 3 cut(s) 458, 681, 800
FaiI YATR 8 cut(s) 242, 263, 365, 456, 679, 753, 787, 798
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FatI CATG 3 cut(s) 454, 677, 796
Fnu4HI GCNGC 2 cut(s) 345, 744
FokI GGATG 1 cut(s) 33
Fsp4HI GCNGC 2 cut(s) 345, 744
FspBI CTAG 3 cut(s) 359, 654, 841
GlaI GCGC 1 cut(s) 125
GluI GCNGC 2 cut(s) 345, 744
GsaI CCCAGC 1 cut(s) 198
HaeIII GGCC 1 cut(s) 247
HapII CCGG 2 cut(s) 76, 447
HgaI GACGC 1 cut(s) 13
HhaI GCGC 1 cut(s) 126
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 3 cut(s) 458, 681, 800
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HinfI GANTC 6 cut(s) 79, 101, 325, 569, 605, 692
HpaII CCGG 2 cut(s) 76, 447
HphI GGTGA 3 cut(s) 179, 614, 656
Hpy166II GTNNAC 2 cut(s) 187, 628
Hpy188I TCNGA 4 cut(s) 9, 585, 590, 685
Hpy188III TCNNGA 6 cut(s) 76, 305, 359, 397, 704, 728
Hpy8I GTNNAC 2 cut(s) 187, 628
HpyAV CCTTC 3 cut(s) 161, 251, 370
HpyCH4III ACNGT 1 cut(s) 776
HpyCH4IV ACGT 2 cut(s) 72, 616
HpyCH4V TGCA 5 cut(s) 62, 230, 502, 622, 800
HpySE526I ACGT 2 cut(s) 72, 616
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 3 cut(s) 458, 681, 800
HspAI GCGC 1 cut(s) 124
Kpn2I TCCGGA 1 cut(s) 75
Kzo9I GATC 4 cut(s) 310, 580, 585, 789
LguI GCTCTTC 1 cut(s) 168
LmnI GCTCC 1 cut(s) 751
Lsp1109I GCAGC 2 cut(s) 331, 730
LweI GCATC 1 cut(s) 11
MaeI CTAG 3 cut(s) 359, 654, 841
MaeII ACGT 2 cut(s) 72, 616
MaeIII GTNAC 1 cut(s) 165
MalI GATC 4 cut(s) 312, 582, 587, 791
MboI GATC 4 cut(s) 310, 580, 585, 789
MboII GAAGA 7 cut(s) 185, 271, 320, 412, 431, 590, 820
MflI RGATCY 3 cut(s) 310, 585, 789
MluCI AATT 6 cut(s) 138, 416, 546, 550, 592, 763
MlyI GAGTC 3 cut(s) 73, 578, 701
MmeI TCCRAC 1 cut(s) 492
MnlI CCTC 5 cut(s) 3, 90, 358, 517, 759
MroI TCCGGA 1 cut(s) 75
MseI TTAA 4 cut(s) 65, 206, 315, 549
MspI CCGG 2 cut(s) 76, 447
MspR9I CCNGG 4 cut(s) 29, 191, 369, 448
MvaI CCWGG 3 cut(s) 29, 191, 369
NciI CCSGG 1 cut(s) 448
NdeII GATC 4 cut(s) 310, 580, 585, 789
NlaIII CATG 3 cut(s) 458, 681, 800
NmuCI GTSAC 1 cut(s) 165
NspV TTCGAA 1 cut(s) 442
PciSI GCTCTTC 1 cut(s) 168
PfeI GAWTC 3 cut(s) 101, 325, 605
PfoI TCCNGGA 1 cut(s) 27
PkrI GCNGC 2 cut(s) 346, 745
PleI GAGTC 3 cut(s) 73, 577, 700
PpsI GAGTC 3 cut(s) 73, 577, 700
PpuMI RGGWCCY 1 cut(s) 12
PshBI ATTAAT 1 cut(s) 549
Psp5II RGGWCCY 1 cut(s) 12
Psp6I CCWGG 3 cut(s) 27, 189, 367
PspFI CCCAGC 1 cut(s) 194
PspGI CCWGG 3 cut(s) 27, 189, 367
PspPI GGNCC 1 cut(s) 12
PspPPI RGGWCCY 1 cut(s) 12
PsuI RGATCY 3 cut(s) 310, 585, 789
RsaI GTAC 2 cut(s) 459, 659
RsaNI GTAC 2 cut(s) 458, 658
SapI GCTCTTC 1 cut(s) 168
SaqAI TTAA 4 cut(s) 65, 206, 315, 549
SatI GCNGC 2 cut(s) 345, 744
Sau3AI GATC 4 cut(s) 310, 580, 585, 789
Sau96I GGNCC 1 cut(s) 12
SchI GAGTC 3 cut(s) 73, 578, 701
ScrFI CCNGG 4 cut(s) 29, 191, 369, 448
SfaNI GCATC 1 cut(s) 11
SfuI TTCGAA 1 cut(s) 442
SinI GGWCC 1 cut(s) 12
SmlI CTYRAG 2 cut(s) 334, 349
SmoI CTYRAG 2 cut(s) 334, 349
Sse9I AATT 6 cut(s) 138, 416, 546, 550, 592, 763
SsiI CCGC 1 cut(s) 18
SspI AATATT 1 cut(s) 560
SspMI CTAG 3 cut(s) 359, 654, 841
StyD4I CCNGG 4 cut(s) 27, 189, 367, 446
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 1 cut(s) 776
TaiI ACGT 2 cut(s) 75, 619
TaqI TCGA 3 cut(s) 396, 442, 690
TasI AATT 6 cut(s) 138, 416, 546, 550, 592, 763
TatI WGTACW 1 cut(s) 457
TfiI GAWTC 3 cut(s) 101, 325, 605
Tru1I TTAA 4 cut(s) 65, 206, 315, 549
Tru9I TTAA 4 cut(s) 65, 206, 315, 549
TseFI GTSAC 1 cut(s) 165
TseI GCWGC 2 cut(s) 344, 743
Tsp45I GTSAC 1 cut(s) 165
TspDTI ATGAA 5 cut(s) 271, 558, 663, 729, 785
TspGWI ACGGA 1 cut(s) 562
VpaK11BI GGWCC 1 cut(s) 12
VspI ATTAAT 1 cut(s) 549
XapI RAATTY 2 cut(s) 416, 592
XbaI TCTAGA 1 cut(s) 358
XspI CTAG 3 cut(s) 359, 654, 841
ZraI GACGTC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.