Rh6BG284400

Belongs to the thiolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0017987
Physical Location & Seq
Reverse (-)
50818150 .. 50821117
2968 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG284400.1

Sequence Viewer

Length: 648 bp
ATGTCAGTTGATAGCATTGGTAGTGTCCGTAGACTTAACCCCAAGGCTGAAGCCTTTGCACAAGCCCGCGACTGTCTTCTTCCTATGGGTGTTACTTCTGAAAATGTTGCACAGCGTTATGGTGTAACACGACAGGAGCAAGACCAAGCTGCTGTGAGTTCTATTAAGTATTTAATATGCATTTGGGAATGTCCGGATTATCTGAGCACAGATATTCAAAGCTTGACCTTTGCTTCTTCAAAATGCTTAGATATACCGGAGCTGCAGACTGTCAAAACAATTTTTGAGAAGAAATTCGGTACAGATTTTGTGGCTGTTGCTATAAATCTTCTACCTGGGTGTAATGTACATCAGGAGATGAGATCGTTGCTAGCTACTGAACCCCCCACCCTTGCAAAAAAGTTGGAAACATTGAAGAAAATAGCAAAGGAGAATAACATTGATTGGTCATATTCCAAAGAAACTCTTGAATATGAGCATTGGGATACGGAGTCTGAGTTTGAAGATAGCAACGAATATTTATATCCGGCCCCAACTCTCGCTGCTCCTCATATACATCCTAAATTACCAGACTACGAGAACTTAAGTCGCTTCTTTCATGACCTGTTCACAGAGCAAAAGCGAGGGTCCATAGAGAAACTAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003988 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0006950 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009062 GO:0009507 GO:0009514 GO:0009536 GO:0009611 GO:0009657 GO:0009694 GO:0009695 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010111 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016042 GO:0016043 GO:0016053 GO:0016054 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019395 GO:0019752 GO:0023051 GO:0023056 GO:0030258 GO:0031090 GO:0031974 GO:0031981 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046395 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055114 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072330 GO:0098588 GO:0098805 GO:1901419 GO:1901421 GO:1901575 GO:1901576 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko00071 ko00280 ko00592 ko01040 ko01100 ko01110 ko01130 ko01212 ko03320 ko04146 map00071 map00280 map00592 map01040 map01100 map01110 map01130 map01212 map03320 map04146
Pfam Domains
Protein Families
Thiolase_C Thiolase_N

Protein Analysis

215

Amino Acids

24.48

Weight (kDa)

5.11

Isoelectric Point (pI)

48.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 54 - 148 6.4e-18 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017987)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g19470
rosa_laevigata RLG00000012664
rosa_multiflora Rmu_sc0002535.1_g000006
rosa_roxburghii Rroxscaffold_7G00183450 Rroxscaffold_7G00183460 Rroxscaffold_7G00183570
rosa_samantha Rh6BG283800 Rh6BG284300 Rh6BG284400
rosa_wichuraiana Rw0G000380 Rw6G024340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 31
AccII CGCG 1 cut(s) 69
AccIII TCCGGA 1 cut(s) 193
AciI CCGC 1 cut(s) 67
AcsI RAATTY 1 cut(s) 293
AcuI CTGAAG 1 cut(s) 69
AfaI GTAC 2 cut(s) 301, 348
AflII CTTAAG 1 cut(s) 583
AgsI TTSAA 5 cut(s) 218, 240, 415, 470, 503
AjnI CCWGG 1 cut(s) 334
AluBI AGCT 4 cut(s) 149, 222, 262, 374
AluI AGCT 4 cut(s) 149, 222, 262, 374
Alw21I GWGCWC 1 cut(s) 209
Aor13HI TCCGGA 1 cut(s) 193
AoxI GGCC 1 cut(s) 528
ApeKI GCWGC 3 cut(s) 149, 262, 542
ApoI RAATTY 1 cut(s) 293
Asp700I GAANNNNTTC 1 cut(s) 293
AspS9I GGNCC 2 cut(s) 529, 627
AsuNHI GCTAGC 1 cut(s) 370
AvaII GGWCC 1 cut(s) 627
BbsI GAAGAC 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 209
BbvI GCAGC 3 cut(s) 136, 249, 529
BciT130I CCWGG 1 cut(s) 336
BciVI GTATCC 1 cut(s) 478
BfaI CTAG 1 cut(s) 371
BfmI CTRYAG 1 cut(s) 263
BfrI CTTAAG 1 cut(s) 583
BfuI GTATCC 1 cut(s) 478
BisI GCNGC 3 cut(s) 150, 263, 543
BlsI GCNGC 3 cut(s) 151, 264, 544
Bme1390I CCNGG 1 cut(s) 336
Bme18I GGWCC 1 cut(s) 627
BmgT120I GGNCC 2 cut(s) 529, 627
BmiI GGNNCC 2 cut(s) 531, 628
BmrFI CCNGG 1 cut(s) 336
BmtI GCTAGC 1 cut(s) 374
BpiI GAAGAC 1 cut(s) 68
BsaJI CCNNGG 2 cut(s) 42, 335
BsaWI WCCGGW 2 cut(s) 193, 256
BsaXI ACNNNNNCTCC 2 cut(s) 482, 512
BseAI TCCGGA 1 cut(s) 193
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 42, 335
BseGI GGATG 1 cut(s) 556
BseMII CTCAG 2 cut(s) 194, 486
BseRI GAGGAG 1 cut(s) 537
BseXI GCAGC 3 cut(s) 136, 249, 529
Bsh1236I CGCG 1 cut(s) 69
BshFI GGCC 1 cut(s) 530
BsiHKAI GWGCWC 1 cut(s) 209
BsiSI CCGG 3 cut(s) 194, 257, 527
BsnI GGCC 1 cut(s) 530
Bsp1286I GDGCHC 1 cut(s) 209
Bsp13I TCCGGA 1 cut(s) 193
Bsp1407I TGTACA 1 cut(s) 346
Bsp143I GATC 1 cut(s) 362
BspACI CCGC 1 cut(s) 67
BspANI GGCC 1 cut(s) 530
BspCNI CTCAG 2 cut(s) 195, 487
BspEI TCCGGA 1 cut(s) 193
BspFNI CGCG 1 cut(s) 69
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 2 cut(s) 531, 628
BspMAI CTGCAG 1 cut(s) 267
BspOI GCTAGC 1 cut(s) 374
BspTI CTTAAG 1 cut(s) 583
BsrGI TGTACA 1 cut(s) 346
BssECI CCNNGG 2 cut(s) 42, 335
BssMI GATC 1 cut(s) 362
BssT1I CCWWGG 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 2 cut(s) 74, 271
BstAFI CTTAAG 1 cut(s) 583
BstAUI TGTACA 1 cut(s) 346
BstC8I GCNNGC 2 cut(s) 67, 372
BstDEI CTNAG 3 cut(s) 203, 247, 495
BstF5I GGATG 1 cut(s) 556
BstFNI CGCG 1 cut(s) 69
BstKTI GATC 1 cut(s) 365
BstMBI GATC 1 cut(s) 362
BstNI CCWGG 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 334
BstSFI CTRYAG 1 cut(s) 263
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 3 cut(s) 136, 249, 529
BstV2I GAAGAC 1 cut(s) 68
BsuI GTATCC 1 cut(s) 478
BsuRI GGCC 1 cut(s) 530
BtsCI GGATG 1 cut(s) 556
Cac8I GCNNGC 2 cut(s) 67, 372
CciI TCATGA 1 cut(s) 598
Cfr13I GGNCC 2 cut(s) 529, 627
Csp6I GTAC 2 cut(s) 300, 347
CviAII CATG 1 cut(s) 599
CviJI RGCY 9 cut(s) 47, 53, 65, 149, 222, 262, 314, 374, 530
CviKI_1 RGCY 9 cut(s) 47, 53, 65, 149, 222, 262, 314, 374, 530
CviQI GTAC 2 cut(s) 300, 347
DdeI CTNAG 3 cut(s) 203, 247, 495
DpnI GATC 1 cut(s) 364
DpnII GATC 1 cut(s) 362
Eco130I CCWWGG 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 627
Eco57I CTGAAG 1 cut(s) 69
EcoRII CCWGG 1 cut(s) 334
EcoT14I CCWWGG 1 cut(s) 42
EcoT22I ATGCAT 1 cut(s) 182
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 1 cut(s) 602
FalI AAGNNNNNCTT 2 cut(s) 450, 482
FatI CATG 1 cut(s) 598
FauI CCCGC 1 cut(s) 74
FblI GTMKAC 1 cut(s) 31
Fnu4HI GCNGC 3 cut(s) 150, 263, 543
FokI GGATG 1 cut(s) 543
Fsp4HI GCNGC 3 cut(s) 150, 263, 543
FspBI CTAG 1 cut(s) 371
GluI GCNGC 3 cut(s) 150, 263, 543
HaeIII GGCC 1 cut(s) 530
HapII CCGG 3 cut(s) 194, 257, 527
Hin1II CATG 1 cut(s) 602
HindIII AAGCTT 1 cut(s) 220
HinfI GANTC 1 cut(s) 491
HpaII CCGG 3 cut(s) 194, 257, 527
Hpy166II GTNNAC 2 cut(s) 32, 609
Hpy188I TCNGA 3 cut(s) 100, 204, 496
Hpy188III TCNNGA 4 cut(s) 194, 353, 467, 599
Hpy8I GTNNAC 2 cut(s) 32, 609
HpyCH4III ACNGT 2 cut(s) 74, 271
HpyCH4V TGCA 5 cut(s) 59, 110, 180, 265, 395
HpyF3I CTNAG 3 cut(s) 203, 247, 495
Hsp92II CATG 1 cut(s) 602
Kpn2I TCCGGA 1 cut(s) 193
Kzo9I GATC 1 cut(s) 362
LmnI GCTCC 3 cut(s) 136, 259, 550
LpnPI CCDG 9 cut(s) 119, 207, 270, 321, 338, 348, 540, 582, 617
Lsp1109I GCAGC 3 cut(s) 136, 249, 529
MaeI CTAG 1 cut(s) 371
MaeIII GTNAC 2 cut(s) 91, 124
MalI GATC 1 cut(s) 364
MboI GATC 1 cut(s) 362
MboII GAAGA 7 cut(s) 68, 71, 228, 301, 320, 427, 515
MhlI GDGCHC 1 cut(s) 209
MluCI AATT 3 cut(s) 279, 293, 563
MlyI GAGTC 1 cut(s) 500
MmeI TCCRAC 1 cut(s) 384
MnlI CCTC 2 cut(s) 558, 617
Mph1103I ATGCAT 1 cut(s) 182
MroI TCCGGA 1 cut(s) 193
MroXI GAANNNNTTC 1 cut(s) 293
MseI TTAA 4 cut(s) 36, 165, 173, 584
MspCI CTTAAG 1 cut(s) 583
MspI CCGG 3 cut(s) 194, 257, 527
MspR9I CCNGG 1 cut(s) 336
MvaI CCWGG 1 cut(s) 336
MvnI CGCG 1 cut(s) 69
NdeII GATC 1 cut(s) 362
NheI GCTAGC 1 cut(s) 370
NlaIII CATG 1 cut(s) 602
NlaIV GGNNCC 2 cut(s) 531, 628
NsiI ATGCAT 1 cut(s) 182
PagI TCATGA 1 cut(s) 598
PdmI GAANNNNTTC 1 cut(s) 293
PkrI GCNGC 3 cut(s) 151, 264, 544
PleI GAGTC 1 cut(s) 499
PpsI GAGTC 1 cut(s) 499
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 2 cut(s) 531, 628
PspPI GGNCC 2 cut(s) 529, 627
PstI CTGCAG 1 cut(s) 267
RsaI GTAC 2 cut(s) 301, 348
RsaNI GTAC 2 cut(s) 300, 347
SaqAI TTAA 4 cut(s) 36, 165, 173, 584
SatI GCNGC 3 cut(s) 150, 263, 543
Sau3AI GATC 1 cut(s) 362
Sau96I GGNCC 2 cut(s) 529, 627
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 1 cut(s) 209
SetI ASST 7 cut(s) 151, 224, 230, 264, 337, 376, 606
SfcI CTRYAG 1 cut(s) 263
SinI GGWCC 1 cut(s) 627
SmlI CTYRAG 1 cut(s) 583
SmoI CTYRAG 1 cut(s) 583
Sse9I AATT 3 cut(s) 279, 293, 563
SsiI CCGC 1 cut(s) 67
SspI AATATT 1 cut(s) 518
SspMI CTAG 1 cut(s) 371
StyD4I CCNGG 1 cut(s) 334
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 2 cut(s) 74, 271
TasI AATT 3 cut(s) 279, 293, 563
TatI WGTACW 1 cut(s) 346
Tru1I TTAA 4 cut(s) 36, 165, 173, 584
Tru9I TTAA 4 cut(s) 36, 165, 173, 584
TseI GCWGC 3 cut(s) 149, 262, 542
TspDTI ATGAA 1 cut(s) 587
TspGWI ACGGA 2 cut(s) 17, 503
Vha464I CTTAAG 1 cut(s) 583
VpaK11BI GGWCC 1 cut(s) 627
XapI RAATTY 1 cut(s) 293
XmiI GTMKAC 1 cut(s) 31
XmnI GAANNNNTTC 1 cut(s) 293
XspI CTAG 1 cut(s) 371
Zsp2I ATGCAT 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.