Rw6G024340

Regulator of Vps4 activity in the MVB pathway protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0017987
Physical Location & Seq
Forward (+)
47285991 .. 47289987
3997 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G024340.1

Sequence Viewer

Length: 846 bp
ATGTTTCTTAGGGCCTGCGGATGCGTCCACGACGAGGAGTTCTTGTTTGACAGATGGGAATCTGCATTAAATGACGTCCGGGCTCAAACAAACGCTTTGAGGGAATCAAAACTGAAAACAATACAGCGCATTAGAAAAGAAATCGCCTGTTCCCTTCGCCAAGGTCAGTCACAAGAAGAGCAGGCTGTTCACCAGGCTGGGCAGTTATTAAAAGAGATGGGGTCTTACAATGCACTTCAAATCATACGGCCATCTTGTGAAGGCATACTTATCAGGCTTATCTTCATCGCAAGGCAAAGGAAGTGTCCTGAAGATCTTAAAATGGAGATTCAAAACTTGAGCTTTGCTGCTTCAAGGTGTCTAGATATACCAGGGCTGAAGGCTCTCAAAGACATTTTCGAGAAGAGATTTGGGAAAAAATTTGTGGCTGAAGCTACAAATCTTCGAACCGGGTGTCATGTACAAAGAGAGCTGATAACAATGCTATCTACTGAACACTTACTGCAAAAAAAGTTGGAACTATTGGAGGAAATAGCCAAGGACAATGAAATTAATTGGAAATATTCTAAAGAGTCTGTTGAAGATCAGATCCGAAAATTTTTTGGTGGTTCTGCTAAACGTGTGCAAGTAAACATTGAGCCTAATGGCGATGGAACTAGGTACTTTCACCAAACAACTCCATGTTCAGATGTCGAGTCTGTTTATCAAGATTGTGATGAATATCTCTATCCAGAGCCATCTCCTGCTGCTCCTCATACACACCCTAATTTGCCAGACTGTGATAATGTATGGATCTTTCTTGCAGAACAGAAGAAACATTACCAAGAACACAAACCTAAAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

281

Amino Acids

32.51

Weight (kDa)

6.76

Isoelectric Point (pI)

54.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 28 - 185 1e-26 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017987)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g19470
rosa_laevigata RLG00000012664
rosa_multiflora Rmu_sc0002535.1_g000006
rosa_roxburghii Rroxscaffold_7G00183450 Rroxscaffold_7G00183460 Rroxscaffold_7G00183570
rosa_samantha Rh6BG283800 Rh6BG284300 Rh6BG284400
rosa_wichuraiana Rw0G000380 Rw6G024340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 78
AciI CCGC 1 cut(s) 18
AclWI GGATC 2 cut(s) 583, 800
AcoI YGGCCR 1 cut(s) 248
AcsI RAATTY 2 cut(s) 419, 596
AcuI CTGAAG 3 cut(s) 330, 398, 450
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 2 cut(s) 462, 662
AfiI CCNNNNNNNGG 1 cut(s) 34
AflIII ACRYGT 1 cut(s) 619
AgsI TTSAA 4 cut(s) 239, 332, 354, 581
AjnI CCWGG 2 cut(s) 192, 370
AluBI AGCT 3 cut(s) 342, 434, 472
AluI AGCT 3 cut(s) 342, 434, 472
AlwI GGATC 2 cut(s) 583, 800
AoxI GGCC 2 cut(s) 12, 248
ApeKI GCWGC 2 cut(s) 347, 746
ApoI RAATTY 2 cut(s) 419, 596
ArsI GACNNNNNNTTYG 4 cut(s) 289, 321, 439, 471
AseI ATTAAT 1 cut(s) 552
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 1 cut(s) 12
AsuC2I CCSGG 2 cut(s) 80, 451
AsuHPI GGTGA 2 cut(s) 182, 659
AsuII TTCGAA 1 cut(s) 445
BanII GRGCYC 1 cut(s) 85
BarI GAAGNNNNNNTAC 2 cut(s) 803, 835
BbvI GCAGC 2 cut(s) 334, 733
BccI CCATC 5 cut(s) 48, 211, 259, 644, 745
BceAI ACGGC 1 cut(s) 263
BciT130I CCWGG 2 cut(s) 194, 372
BcnI CCSGG 2 cut(s) 80, 451
BfaI CTAG 3 cut(s) 362, 657, 844
BglII AGATCT 1 cut(s) 313
BisI GCNGC 2 cut(s) 348, 747
BlsI GCNGC 2 cut(s) 349, 748
Bme1390I CCNGG 4 cut(s) 80, 194, 372, 451
BmgT120I GGNCC 1 cut(s) 12
BmrFI CCNGG 4 cut(s) 80, 194, 372, 451
BmsI GCATC 1 cut(s) 11
Bpu14I TTCGAA 1 cut(s) 445
BpuEI CTTGAG 1 cut(s) 358
BpuMI CCSGG 2 cut(s) 80, 451
BsaBI GATNNNNATC 2 cut(s) 58, 720
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 160, 371, 537
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse8I GATNNNNATC 2 cut(s) 58, 720
BseBI CCWGG 2 cut(s) 194, 372
BseDI CCNNGG 3 cut(s) 160, 371, 537
BseGI GGATG 1 cut(s) 26
BseJI GATNNNNATC 2 cut(s) 58, 720
BseLI CCNNNNNNNGG 1 cut(s) 34
BseRI GAGGAG 2 cut(s) 50, 741
BseXI GCAGC 2 cut(s) 334, 733
BseYI CCCAGC 1 cut(s) 197
BshFI GGCC 2 cut(s) 14, 250
BsiSI CCGG 2 cut(s) 79, 450
BslI CCNNNNNNNGG 1 cut(s) 34
BsnI GGCC 2 cut(s) 14, 250
Bsp119I TTCGAA 1 cut(s) 445
Bsp1286I GDGCHC 1 cut(s) 85
Bsp1407I TGTACA 1 cut(s) 460
Bsp143I GATC 4 cut(s) 313, 583, 588, 792
BspACI CCGC 1 cut(s) 18
BspANI GGCC 2 cut(s) 14, 250
BspPI GGATC 2 cut(s) 583, 800
BspQI GCTCTTC 1 cut(s) 171
BspT104I TTCGAA 1 cut(s) 445
BsrGI TGTACA 1 cut(s) 460
BssECI CCNNGG 3 cut(s) 160, 371, 537
BssMI GATC 4 cut(s) 313, 583, 588, 792
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 2 cut(s) 160, 537
Bst2UI CCWGG 2 cut(s) 194, 372
Bst4CI ACNGT 1 cut(s) 779
Bst6I CTCTTC 2 cut(s) 171, 398
BstACI GRCGYC 1 cut(s) 75
BstAUI TGTACA 1 cut(s) 460
BstBI TTCGAA 1 cut(s) 445
BstC8I GCNNGC 2 cut(s) 16, 183
BstDEI CTNAG 1 cut(s) 8
BstF5I GGATG 1 cut(s) 26
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 4 cut(s) 316, 586, 591, 795
BstMBI GATC 4 cut(s) 313, 583, 588, 792
BstNI CCWGG 2 cut(s) 194, 372
BstSCI CCNGG 4 cut(s) 78, 192, 370, 449
BstV1I GCAGC 2 cut(s) 334, 733
BstX2I RGATCY 3 cut(s) 313, 588, 792
BstYI RGATCY 3 cut(s) 313, 588, 792
BsuRI GGCC 2 cut(s) 14, 250
BtgZI GCGATG 2 cut(s) 271, 663
BtsCI GGATG 1 cut(s) 26
Cac8I GCNNGC 2 cut(s) 16, 183
CfoI GCGC 1 cut(s) 129
Cfr13I GGNCC 1 cut(s) 12
CseI GACGC 1 cut(s) 13
Csp6I GTAC 2 cut(s) 461, 661
CviAII CATG 2 cut(s) 458, 681
CviQI GTAC 2 cut(s) 461, 661
DdeI CTNAG 1 cut(s) 8
DpnI GATC 4 cut(s) 315, 585, 590, 794
DpnII GATC 4 cut(s) 313, 583, 588, 792
EaeI YGGCCR 1 cut(s) 248
Eam1104I CTCTTC 2 cut(s) 171, 398
EarI CTCTTC 2 cut(s) 171, 398
Eco130I CCWWGG 2 cut(s) 160, 537
Eco24I GRGCYC 1 cut(s) 85
Eco57I CTGAAG 3 cut(s) 330, 398, 450
EcoO109I RGGNCCY 1 cut(s) 12
EcoRII CCWGG 2 cut(s) 192, 370
EcoT14I CCWWGG 2 cut(s) 160, 537
EcoT38I GRGCYC 1 cut(s) 85
ErhI CCWWGG 2 cut(s) 160, 537
FaeI CATG 2 cut(s) 461, 684
FaiI YATR 7 cut(s) 245, 266, 368, 459, 682, 756, 790
FalI AAGNNNNNCTT 2 cut(s) 252, 284
FatI CATG 2 cut(s) 457, 680
Fnu4HI GCNGC 2 cut(s) 348, 747
FokI GGATG 1 cut(s) 33
FriOI GRGCYC 1 cut(s) 85
Fsp4HI GCNGC 2 cut(s) 348, 747
FspBI CTAG 3 cut(s) 362, 657, 844
GlaI GCGC 1 cut(s) 128
GluI GCNGC 2 cut(s) 348, 747
GsaI CCCAGC 1 cut(s) 201
HaeIII GGCC 2 cut(s) 14, 250
HapII CCGG 2 cut(s) 79, 450
HgaI GACGC 1 cut(s) 13
HhaI GCGC 1 cut(s) 129
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 2 cut(s) 461, 684
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HinfI GANTC 5 cut(s) 59, 104, 328, 572, 695
HpaII CCGG 2 cut(s) 79, 450
HphI GGTGA 2 cut(s) 182, 659
Hpy166II GTNNAC 3 cut(s) 28, 190, 631
Hpy188I TCNGA 3 cut(s) 588, 593, 688
Hpy188III TCNNGA 5 cut(s) 308, 362, 400, 707, 731
Hpy8I GTNNAC 3 cut(s) 28, 190, 631
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 3 cut(s) 164, 254, 373
HpyCH4III ACNGT 1 cut(s) 779
HpyCH4IV ACGT 2 cut(s) 75, 619
HpyCH4V TGCA 5 cut(s) 65, 233, 505, 625, 803
HpyF3I CTNAG 1 cut(s) 8
HpySE526I ACGT 2 cut(s) 75, 619
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 2 cut(s) 461, 684
HspAI GCGC 1 cut(s) 127
Kzo9I GATC 4 cut(s) 313, 583, 588, 792
LguI GCTCTTC 1 cut(s) 171
LmnI GCTCC 1 cut(s) 754
Lsp1109I GCAGC 2 cut(s) 334, 733
LweI GCATC 1 cut(s) 11
MaeI CTAG 3 cut(s) 362, 657, 844
MaeII ACGT 2 cut(s) 75, 619
MaeIII GTNAC 1 cut(s) 168
MalI GATC 4 cut(s) 315, 585, 590, 794
MboI GATC 4 cut(s) 313, 583, 588, 792
MboII GAAGA 7 cut(s) 188, 274, 323, 415, 434, 593, 823
MflI RGATCY 3 cut(s) 313, 588, 792
MhlI GDGCHC 1 cut(s) 85
MluCI AATT 5 cut(s) 419, 549, 553, 596, 766
MlyI GAGTC 2 cut(s) 581, 704
MmeI TCCRAC 1 cut(s) 495
MnlI CCTC 4 cut(s) 28, 93, 520, 762
MseI TTAA 4 cut(s) 68, 209, 318, 552
MspI CCGG 2 cut(s) 79, 450
MspR9I CCNGG 4 cut(s) 80, 194, 372, 451
MvaI CCWGG 2 cut(s) 194, 372
NciI CCSGG 2 cut(s) 80, 451
NdeII GATC 4 cut(s) 313, 583, 588, 792
NlaIII CATG 2 cut(s) 461, 684
NmuCI GTSAC 1 cut(s) 168
NspV TTCGAA 1 cut(s) 445
PciSI GCTCTTC 1 cut(s) 171
PfeI GAWTC 3 cut(s) 59, 104, 328
PkrI GCNGC 2 cut(s) 349, 748
PleI GAGTC 2 cut(s) 580, 703
PpsI GAGTC 2 cut(s) 580, 703
PshBI ATTAAT 1 cut(s) 552
Psp6I CCWGG 2 cut(s) 192, 370
PspFI CCCAGC 1 cut(s) 197
PspGI CCWGG 2 cut(s) 192, 370
PspPI GGNCC 1 cut(s) 12
PsuI RGATCY 3 cut(s) 313, 588, 792
RsaI GTAC 2 cut(s) 462, 662
RsaNI GTAC 2 cut(s) 461, 661
SapI GCTCTTC 1 cut(s) 171
SaqAI TTAA 4 cut(s) 68, 209, 318, 552
SatI GCNGC 2 cut(s) 348, 747
Sau3AI GATC 4 cut(s) 313, 583, 588, 792
Sau96I GGNCC 1 cut(s) 12
SchI GAGTC 2 cut(s) 581, 704
ScrFI CCNGG 4 cut(s) 80, 194, 372, 451
SduI GDGCHC 1 cut(s) 85
SetI ASST 9 cut(s) 78, 166, 344, 359, 436, 474, 622, 662, 838
SfaNI GCATC 1 cut(s) 11
SfuI TTCGAA 1 cut(s) 445
SmlI CTYRAG 1 cut(s) 337
SmoI CTYRAG 1 cut(s) 337
Sse9I AATT 5 cut(s) 419, 549, 553, 596, 766
SsiI CCGC 1 cut(s) 18
SspI AATATT 1 cut(s) 563
SspMI CTAG 3 cut(s) 362, 657, 844
StyD4I CCNGG 4 cut(s) 78, 192, 370, 449
StyI CCWWGG 2 cut(s) 160, 537
TaaI ACNGT 1 cut(s) 779
TaiI ACGT 2 cut(s) 78, 622
TaqI TCGA 3 cut(s) 399, 445, 693
TasI AATT 5 cut(s) 419, 549, 553, 596, 766
TatI WGTACW 1 cut(s) 460
TfiI GAWTC 3 cut(s) 59, 104, 328
Tru1I TTAA 4 cut(s) 68, 209, 318, 552
Tru9I TTAA 4 cut(s) 68, 209, 318, 552
TseFI GTSAC 1 cut(s) 168
TseI GCWGC 2 cut(s) 347, 746
Tsp45I GTSAC 1 cut(s) 168
TspDTI ATGAA 3 cut(s) 274, 561, 732
VspI ATTAAT 1 cut(s) 552
XapI RAATTY 2 cut(s) 419, 596
XbaI TCTAGA 1 cut(s) 361
XspI CTAG 3 cut(s) 362, 657, 844
ZraI GACGTC 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.