Rroxscaffold_7G00199770

RIO-like kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0018549
Physical Location & Seq
Reverse (-)
46954538 .. 46959156
4619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00199770.1

Sequence Viewer

Length: 429 bp
ATGGCGCTCTTCCGATTTCCTGCTGTTGAGTTGGCGCGGATCTCGGGTTTTAATAGCGGCTTCCTCCGAGCTCGCTACGGTGGTGGTGTGGTGATTGAAGAAATCCGGAGAGCAGCGCGAGATGATCTTGAGGGATGTGTGCGGAGGCGGAGGTTACCTGGCCTACATGTATTATACGAGCCATCTAGAGAGATAGATATGTACAATGAGACCGCGCCGATACGAAAGGGATACCGCAAGCACAACCGCAGGGAAATGGTGAAGATATGGGCTGAGAAAGAAAAGAGGAATCTGGACAGGATGGTGAAAAATGGAATTACATGCCCAACTGCAATTGATATAAAAGATCATGTTCTGGTCATGTTTTGTTATGGAGAACGCCTGTGGATTCCACTTACTCCTAGAATGGTAGACATGCTCAGTAAATAA

Protein Analysis

142

Amino Acids

16.65

Weight (kDa)

9.95

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RIO1 PF01163 63 - 123 3.3e-13 RIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018549)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0349911 RchiOBHm_Chr6g0293461 RchiOBHm_Chr7g0228531
rosa_laevigata RLG00000002912
rosa_roxburghii Rroxscaffold_7G00199770
rosa_samantha Rh1AG078400 Rh1CG122900 Rh5BG508100 Rh6CG161700 Rh6CG362500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 411
AccII CGCG 3 cut(s) 37, 118, 215
AccIII TCCGGA 1 cut(s) 105
AciI CCGC 7 cut(s) 37, 57, 142, 148, 213, 235, 247
AclWI GGATC 1 cut(s) 47
AfaI GTAC 1 cut(s) 203
AflIII ACRYGT 1 cut(s) 166
AgsI TTSAA 1 cut(s) 98
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 1 cut(s) 71
AluI AGCT 1 cut(s) 71
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 1 cut(s) 203
AlwI GGATC 1 cut(s) 47
Ama87I CYCGRG 1 cut(s) 43
Aor13HI TCCGGA 1 cut(s) 105
AoxI GGCC 1 cut(s) 160
ApeKI GCWGC 1 cut(s) 113
AspLEI GCGC 4 cut(s) 7, 37, 118, 217
AsuHPI GGTGA 3 cut(s) 103, 271, 316
AvaI CYCGRG 1 cut(s) 43
BanII GRGCYC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 1 cut(s) 125
BccI CCATC 2 cut(s) 190, 295
BciT130I CCWGG 1 cut(s) 159
BciVI GTATCC 1 cut(s) 224
BcoDI GTCTC 1 cut(s) 203
BfaI CTAG 2 cut(s) 186, 402
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 224
BisI GCNGC 2 cut(s) 58, 114
BlsI GCNGC 2 cut(s) 59, 115
Bme1390I CCNGG 1 cut(s) 159
BmeT110I CYCGRG 1 cut(s) 43
BmrFI CCNGG 1 cut(s) 159
BpuEI CTTGAG 1 cut(s) 149
BsaI GGTCTC 1 cut(s) 203
BsaWI WCCGGW 1 cut(s) 105
BseAI TCCGGA 1 cut(s) 105
BseBI CCWGG 1 cut(s) 159
BseGI GGATG 2 cut(s) 140, 306
BseMII CTCAG 1 cut(s) 264
BseXI GCAGC 1 cut(s) 125
Bsh1236I CGCG 3 cut(s) 37, 118, 215
BshFI GGCC 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 73
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 1 cut(s) 106
BsmAI GTCTC 1 cut(s) 203
BsnI GGCC 1 cut(s) 162
Bso31I GGTCTC 1 cut(s) 203
BsoBI CYCGRG 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 73
Bsp13I TCCGGA 1 cut(s) 105
Bsp1407I TGTACA 1 cut(s) 201
Bsp143I GATC 3 cut(s) 39, 124, 346
BspACI CCGC 7 cut(s) 37, 57, 142, 148, 213, 235, 247
BspANI GGCC 1 cut(s) 162
BspCNI CTCAG 1 cut(s) 265
BspEI TCCGGA 1 cut(s) 105
BspFNI CGCG 3 cut(s) 37, 118, 215
BspPI GGATC 1 cut(s) 47
BspQI GCTCTTC 1 cut(s) 14
BspTNI GGTCTC 1 cut(s) 203
BsrGI TGTACA 1 cut(s) 201
BssMI GATC 3 cut(s) 39, 124, 346
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 1 cut(s) 80
Bst6I CTCTTC 1 cut(s) 14
BstAUI TGTACA 1 cut(s) 201
BstC8I GCNNGC 2 cut(s) 73, 239
BstDEI CTNAG 2 cut(s) 273, 419
BstEII GGTNACC 1 cut(s) 153
BstF5I GGATG 2 cut(s) 140, 306
BstFNI CGCG 3 cut(s) 37, 118, 215
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 4 cut(s) 7, 37, 118, 217
BstKTI GATC 3 cut(s) 42, 127, 349
BstMAI GTCTC 1 cut(s) 203
BstMBI GATC 3 cut(s) 39, 124, 346
BstNI CCWGG 1 cut(s) 159
BstNSI RCATGY 3 cut(s) 170, 324, 418
BstPI GGTNACC 1 cut(s) 153
BstSCI CCNGG 1 cut(s) 157
BstUI CGCG 3 cut(s) 37, 118, 215
BstV1I GCAGC 1 cut(s) 125
BstX2I RGATCY 1 cut(s) 39
BstYI RGATCY 1 cut(s) 39
BsuI GTATCC 1 cut(s) 224
BsuRI GGCC 1 cut(s) 162
BtsCI GGATG 2 cut(s) 140, 306
Cac8I GCNNGC 2 cut(s) 73, 239
CfoI GCGC 4 cut(s) 7, 37, 118, 217
Csp6I GTAC 1 cut(s) 202
CviAII CATG 5 cut(s) 167, 321, 350, 361, 415
CviJI RGCY 5 cut(s) 60, 71, 162, 181, 272
CviKI_1 RGCY 5 cut(s) 60, 71, 162, 181, 272
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 2 cut(s) 273, 419
DpnI GATC 3 cut(s) 41, 126, 348
DpnII GATC 3 cut(s) 39, 124, 346
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 73
Eco31I GGTCTC 1 cut(s) 203
Eco53kI GAGCTC 1 cut(s) 71
Eco88I CYCGRG 1 cut(s) 43
Eco91I GGTNACC 1 cut(s) 153
EcoICRI GAGCTC 1 cut(s) 71
EcoO65I GGTNACC 1 cut(s) 153
EcoRII CCWGG 1 cut(s) 157
EcoT38I GRGCYC 1 cut(s) 73
FaeI CATG 5 cut(s) 170, 324, 353, 364, 418
FatI CATG 5 cut(s) 166, 320, 349, 360, 414
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 2 cut(s) 58, 114
FokI GGATG 2 cut(s) 147, 313
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 2 cut(s) 58, 114
FspBI CTAG 2 cut(s) 186, 402
GlaI GCGC 4 cut(s) 6, 36, 117, 216
GluI GCNGC 2 cut(s) 58, 114
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 162
HapII CCGG 1 cut(s) 106
HhaI GCGC 4 cut(s) 7, 37, 118, 217
Hin1II CATG 5 cut(s) 170, 324, 353, 364, 418
Hin6I GCGC 4 cut(s) 5, 35, 116, 215
HinP1I GCGC 4 cut(s) 5, 35, 116, 215
HinfI GANTC 2 cut(s) 289, 388
HpaII CCGG 1 cut(s) 106
HphI GGTGA 3 cut(s) 103, 271, 316
Hpy166II GTNNAC 1 cut(s) 412
Hpy188I TCNGA 2 cut(s) 14, 68
Hpy188III TCNNGA 4 cut(s) 106, 128, 186, 293
Hpy8I GTNNAC 1 cut(s) 412
HpyCH4III ACNGT 1 cut(s) 80
HpyCH4V TGCA 1 cut(s) 332
HpyF3I CTNAG 2 cut(s) 273, 419
Hsp92II CATG 5 cut(s) 170, 324, 353, 364, 418
HspAI GCGC 4 cut(s) 5, 35, 116, 215
Kpn2I TCCGGA 1 cut(s) 105
Kzo9I GATC 3 cut(s) 39, 124, 346
LguI GCTCTTC 1 cut(s) 14
LpnPI CCDG 9 cut(s) 33, 119, 144, 171, 235, 278, 283, 341, 395
Lsp1109I GCAGC 1 cut(s) 125
MaeI CTAG 2 cut(s) 186, 402
MaeIII GTNAC 1 cut(s) 153
MalI GATC 3 cut(s) 41, 126, 348
MboI GATC 3 cut(s) 39, 124, 346
MboII GAAGA 2 cut(s) 110, 274
MfeI CAATTG 1 cut(s) 333
MflI RGATCY 1 cut(s) 39
MhlI GDGCHC 1 cut(s) 73
MluCI AATT 2 cut(s) 315, 333
MnlI CCTC 5 cut(s) 74, 124, 138, 144, 279
MroI TCCGGA 1 cut(s) 105
MseI TTAA 1 cut(s) 51
MspI CCGG 1 cut(s) 106
MspR9I CCNGG 1 cut(s) 159
MunI CAATTG 1 cut(s) 333
MvaI CCWGG 1 cut(s) 159
MvnI CGCG 3 cut(s) 37, 118, 215
NdeII GATC 3 cut(s) 39, 124, 346
NlaIII CATG 5 cut(s) 170, 324, 353, 364, 418
NspI RCATGY 3 cut(s) 170, 324, 418
PciI ACATGT 1 cut(s) 166
PciSI GCTCTTC 1 cut(s) 14
PfeI GAWTC 2 cut(s) 289, 388
PkrI GCNGC 2 cut(s) 59, 115
PscI ACATGT 1 cut(s) 166
Psp124BI GAGCTC 1 cut(s) 73
Psp6I CCWGG 1 cut(s) 157
PspEI GGTNACC 1 cut(s) 153
PspGI CCWGG 1 cut(s) 157
PsuI RGATCY 1 cut(s) 39
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SacI GAGCTC 1 cut(s) 73
SapI GCTCTTC 1 cut(s) 14
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 2 cut(s) 58, 114
Sau3AI GATC 3 cut(s) 39, 124, 346
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 1 cut(s) 73
SetI ASST 3 cut(s) 73, 155, 160
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 2 cut(s) 315, 333
SsiI CCGC 7 cut(s) 37, 57, 142, 148, 213, 235, 247
SspMI CTAG 2 cut(s) 186, 402
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 1 cut(s) 157
TaaI ACNGT 1 cut(s) 80
TasI AATT 2 cut(s) 315, 333
TatI WGTACW 1 cut(s) 201
TauI GCSGC 1 cut(s) 60
TfiI GAWTC 2 cut(s) 289, 388
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TseI GCWGC 1 cut(s) 113
XbaI TCTAGA 1 cut(s) 185
XceI RCATGY 3 cut(s) 170, 324, 418
XmiI GTMKAC 1 cut(s) 411
XspI CTAG 2 cut(s) 186, 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.