Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0013322

Physical Location & Seq

Genomic Coordinates Forward (+)

40206927 .. 40217853

Gene Structure 10927 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0287500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6816 bp

ATGCAATCGGGTGGAGGAGGAGGAGGTGGGCCCGGGCGGAACCCGGGGGTGGGGCCGGCGGGACGGGCAACGTCGAATTCGTCGGCTGCTTCGCCGTCCTCGTCGTCGTCGGCGGTGTCGACGCCCCACTTGGGGTTTGATTCGGTGCAGCAGCACCAGCATCAGCATCAGCAACAGCAACAGCAGCAGCAATTGGGGTCTAGACAGCCATTGCAACAACAATTTTTAAGAAAGCCTGAAGGGAATGAAGCTCTTCTAGCATATCAAGCTGCTGCTTTCCAAGGAGTTTTGAGTGGGAACAATTTTGTTTCAGCTCCCGGCTCCACTCAAATGCCTCAACAACCTAGGAAGTTCATGGATATGGCTCAACAGCATGGCTCACAGGAGGGGCAGAATAGGAGTCAAGGTGTTGACCAGCAAGTGCTAAATCCAGTCCATCAAGCTTATCTTCAGTATGCTTTTCAGGCTTCACAGCAGAAGTCTGGTTTGGCAATGCAATCTCAGCAGCAACCTAAAATGGGGATGTTGGGGCCTCCATCGGGAAAGGATCAAGACATGCGGTCGGCAAATTTGAAAATGCAGGAATTCAATTCTATGCAGGCAGCTGGCCAGGCACAAGCATCATCATCCAAAAACTTGTCATCAAAGAACTCAACAGAGCATTTTGCTCGTGGAGAAAAGCAGATGGACCGAGGACAGTCACCAGCTTCAGACCAAAGGAGTGAATCTAAGCCTTCAGCCCAGCCAGCAGCTGTAGGACAATTCATGCCTGGAAATCTCATGAGGCCAATGATGGCTCCACAGCAAAGCATGCAAAATATGCCAAACAACCAGATGGCATTGGCTGCTCAGTTGCAAGCATTTGCACTTGAGCACAATATTGATCTTTCACAACCTGGAAATGCCAACTTGATGGCACAGCTGATTCCAATGTTGCAGTCTAGGATGGCTACTCAACAAAAGGCGAATGAGAGCAACATGGGTGTACAATCGTCTTCTGCTCCAGTTTCAAAGCAGCAGGTTACTTCTCCTCAAGTTGCAAGTGAGAGTTCTCCTCATGCTAATTCATCAAGTGATGTATCTGGGCAGTCAGGCTCTGCAAAAGCAAAGCTGACAGTTTCACCTAGCCCTTTTGGCTCAGGTTCAAATAGTGGGATCTTTAACAATAGTAACAACATTCCTATGCAGCAGTTTTCTGGTCACGGCAGAGAAAACCAGATACCTCCTAGGCAGTCAGCTCCTTTTGTAAATGGAATGGCTCCTACCCATCCCACACACCCATCTACAAACACAAGCCAAGGTCTCGATCAATCTCTCCATGTAAAAAATTTACCTAACAATCCAGAAACATTGCAAATGCAATACCCTAGGCAGTTAAATCGTTCTTCTCCACAAGCTGTCGTTCCTAATGATGGGGGTTCGGGCAATCACAATGTTCAGTCGCAAGGTGGACCAGCTCCTCAGGTGCCACAACAACGCCCTGGATTCACCAAACAGCAGCTGCATGTTCTCAAAGCACAAATACTAGCATTTAGGCGAATCAAGAAAGGTGAAGGTACACTCCCTCAAGAACTTCTTCGGGCGATTGCTCCACCACCTCTAGAGCAACAGCTACAGCAGCAAATTCTTCCTGGAGGAAATATTCAGGACAAATCTTCTGGGAAGATTGTAGAAGAACATGCTGTGGAGTCCAGTGAAAAAGATTCTCATTCACAGGCTGTGGCATCAGTGAATGGACTGAACATTTCAAAACAGGAAGCTCTTACAGGAGATGAGAAAGCAACTGTATCAACAGTTAATGTGCATGTAATGCCTGCTGTGCTGAAGGAACCTACTCCTGTGGTGTCTGTGGGAAAAGAACAACATTCTGCTGTAGCTTCAGTTAAGTCGGACCATGAAGTTGAACGTAGTAACCAAAAAGATTCTGTCAGAAGTGATTTTTCTGTAGATAGGGGGAAGTCTGTTGCACCACAAGTTGCTGTATCTGATGCCATGCAACTTAAAAAGCCCGCACAAGCAAGCTCCACACCCCAGCCAAAAGATGCAGGCTCTGCCAGAAAGTATCATGGACCACTGTTTGATTTCCCCTTTTTCACTAGGAAACATGACTCCTTTGGTTCAGCAATGATGGTAAACAACAACAGCAACAACAGCAACAATAATAATTTAACATTGGCATATGATGTCAAAGACCTTCTTTTTGAGGAAGGTGTGGAAGTTCTTAACAAGAAGAGGACAGAAAATATAAAGAAGATTGGTGGTCTCTTGGCGGTAAACTTAGAGAGGAAAAGGATTAGGCCAGATCTTGTTTTGCGGCTGCAAATTGAAGAGAAAAAGCTCCGACTTTTGGATTTTCAGGCACGTTTAAGAGATGAAATTGATCAACAGCAGCAAGAGATAATGGCAATGCCTGACAGGCCTTATCGTAAGTTTGTTCGTCTTTGTGAGCGTCAGCGTATGGAGCTAGCAAGGCAAGTGCAGGCCTCTCAGAAAGCTATCAGAGAGAAGCAACTGAAATCTATTTTCCTGTGGCGTAAAAGGCTTCTTGAAGCTCACTGGTCCATTCGTGATGCACGAACTGCACGCAACAGGGGAGTTGCCAAATATCATGAGAGAATGTTAAGGGAGTTTTCAAAAAGAAAGGATGATGATAGAAGCAGAAGGATGGAAGCATTGAAGAATAATGATGTTGAAAGGTACAGGGAAATGTTGCTAGAACAGCAGACTAGTATTACAGGGGATGCAGCAGAGAGATATGCTGTTCTTTCGTCATTCCTGAGCCAGACAGAGGAATATCTTCATAAGCTTGGGAGTAAAATAACAGCTGCTAAAAATCAGCAGGAAGTGGAAGAGGCAGCAAATGCTGCTGCAGCTGCTGCACGATTGCAGGGTCTGTCCGAGGAAGAAGTTAGGGTGGCAGCTGCTTGTGCTGGAGAGGAAGTATTGATTAGAAATCGGTTTATAGAAATGAATGCACCAAGAGATAGTTCATCCGTCAACAAGTATTATAGTCTTGCACATGCTGTAAATGAAAGGGTCATAAGACAACCATCAATGTTACGGACTGGAACCTTAAGAGACTATCAACTTGTTGGGCTCCAATGGATGCTTTCTCTGTATAACAACAAGTTAAATGGAATATTGGCAGATGAAATGGGTCTTGGGAAAACTGTACAGGTAATGGCATTGATTGCATATTTGATGGAGTTCAAAGGGAACTATGGACCGCATCTCATAATTGTTCCAAACGCTGTTTTGGTAAATTGGAAGAGTGAGTTGCATGCTTGGCTTCCATCTGTCTCCTGCATTTATTATGTTGGGAGCAAGGATCAACGGTCAAAATTGTTTTCTCAAGAGGTTTGCGCCTTGAAGTTTAATGTCCTTGTGACAACTTATGAGTTCATCATGTATGATCGTGCCAAACTATCAAAAATTGATTGGAAGTATATAATAATTGATGAAGCACAGAGGATGAAAGACAGGGAATCCGTTCTAGCACGTGATCTTGATAGATATCGTTGCCAACGGCGCTTGCTTTTGACAGGAACACCATTGCAGAATGATTTGAAGGAACTCTGGTCACTTTTAAATCTGCTTCTTCCAGAAGTTTTTGATAATCGCAAAGCATTTCACGACTGGTTTTCCAAACCGTTTCAAAGGGAGGCTCCTACACCGGATGCGGAGGATGATTGGCTTGAGACTGAGAAAAAGGTTATCATTATCCATCGACTCCATCAAATATTAGAGCCGTTTATGCTCAGGCGGCGGGTTGAAGATGTCGAAGGCGCACTTCCACCTAAGATCTCCATAGTCTTAAGGTGTAGAATGTCGGCTATTCAAAGTGCTGTATATGATTGGATAAAATCAACAGGCACCATTCGTGTTGATCCCGAAGACGAGAAGCTAAGGGTCCAAAAAAATCCACTTTATCAGCCCAAGGTGTATAAAACCTTAAATAATAGGTGTATGGAACTTCGAAAAACTTGCAATCATCCTCTGCTTAATTACCCATACTTCAATGATTTTTCAAAGGATTTTCTCATAAGATCCTGTGGGAAATTGTGGATCCTGGATAGGATCCTAATTAAACTTCAGAGAACAGGGCATCGGGTACTTCTTTTCAGTACCATGACAAAACTCCTTGATATATTGGAGGAATACTTGCAGTGGAGGCGACTTGTGTACAGACGAATTGATGGAACCACCAGTTTAGAAGATCGTGAATCAGCTATAGTGGATTTCAACAGCCCTGATTCTGACTGCTTCATCTTCTTGCTTAGTATTCGTGCAGCTGGTCGGGGTCTTAATCTCCAGTCTGCGGACACAGTTGTCATATATGATCCTGATCCAAACCCTAAAAATGAAGAACAGGCAGTTGCTAGAGCCCACCGTATTGGACAGAAGAGGGAAGTCAAAGTCATTTATATGGAAGCAGTTGTTGACAAAATCCCTAGCCATCAGAAAGAGGATGAACTAAGAACTGGAGGCACTGTTGATTCAGAGGATGACCTTGCTGGTAAGGATCGATATATGGGATCAATTGAGAGCCTCATAAGAAATAATATTCAGCAATACAAGATAGACATGGCTGATGAGGTTATCAATGCTGGGCGTTTTGACCAGAGAACTACTCATGAAGAGCGACGAATGACTCTGGAAACTTTACTGCATGATGATGAGAGGTATCAAGAAACTCTCCATGATGTTCCCTCATTGCAGGAGGTAAATCGCATGATTGCTAGAAGTGAAGAAGAAGTTGAATTGTTTGATCAAATGGACGAAGAATATGACTGGATTGAGGAGATGACGAGGTATGATCAGGTTCCCAAATGGCTTCGTACTAGTACAAGAGAAGTAAATACTGTGATTGCTAGTTTATCGAAACGACCATCAAAGAACACTTTGTTGGGTGGAAATATTGGCTTGGAGTCGAGTGAAGTGGGTTCTGAAACTGAAAGAAAGAGGGGTCGACCCAAGGGAAAAAAGCATCTCAGCTATAAGGAAGTAGATGATGAAACTGGAGAATATTCGGAAGCAAGTTCTGATGAGAGAAATGGATATCCTATGCATGAAGAGGAAGGAGAAGTAGGAGAATTGGAAGATGATGAATATAGTGGTGCTGTTGAGGCAACTCCAGTTGAAGATAAGGAACAAGTGGAAGATGGTCCTGAATGTGATGGTGGGTATGATTATCCACCAGCTTCAGAAAGAGTTGGAAATGACCTCATAGTAGAAGAAGCTGGTTCCTCAGGTTCGTCGTCAGACAGTCGGAGATTGATGCAGCCACTTTCTCCTGTTTCCTCTCAGAAGTTTGGTTCCCTGTCTGCCTTAGATGGTAGGTCAGGATCTATTTCAAAGAGGCTGCCAGATGAAGTAGAGGAAGGGGAGATCGTTGTATCTGGAGATTCTCACATGGAACAGGTACAATCTGGAAGTTGGAATCATGACCGTGACGAATGTGAAGATGAACAGGTTTTGCAGCCCAAGATCAAGAGGAAGCGTAGTATTCGGATTCGCCCTCGTCATACCATAGAAAGGCCTGAAGAGAAGTCTGGTAGTGAGACACAATCTGTCCAACGGGGAGATTCATCTTTGTTTCCTTTCCAAGCGGACCATAAATCTCAAGCACATATAAGGGGTGATCCTGAAATGAAAACATATGGAGAATCCGGTGCTTTGAAGCATGACCAAAGTGATTCATCCTCAAAAAGTCGACGAAATTTGCCTACAAGGAGAGCTGCTAGTGCATCAAAATTGCACCCTTCAGCCAAATCTGGCAGATTGAATAACATGTCTGATCCTGCAGATGCTGCTGATCACTACAGAGAAAATTGGGAAGGGAAAGTTGGGAATACAAGTGGGACTTCAAGTTATGTTACAAAAATGCCTGACATCATACAGAGAAGGTGCAAGAATGTTATTAGCAAGCTCCAGAGGAGAATTGATAAGGAAGGTCCTCAAATAGTACCTTTACTCACAGATTTGTGGAAGCGGATTGAAAATTCTGGTTACACAAGTGGATCGGCAAATAACATCTTGGATTTACGAAAAATTGATCAGCGCATAGAGCGGTTAGAGTATAGTGGAGTGATGGAGCTCGTGTTTGATGTCCAGTCTATGTTGAAAAGTTCAATGCAATATTATGGGTTCTCGCATGAGGTACGAACTGAAGCCAGGAAAGTACACGATCTTTTCTTTGATATCTTGAAAATTGCCTTTGCTGACACTGACTTTCGAGAAGCCAGAAGTGCACTCTCTTTTACCAGCCCAGTTGTGACCACCAATGCTCTATCCCCAAGACCGGGTGTTGGACAAAGCAAGAGACACAAATTGATAAATGAGGTGGAACCTGACCCCAGTCCTCAACAGAAGCCACTACGGGGACCTATTATCAGTAGTGAAGAAACAAGGGTAAGAAGCCATATGCCCCAGAAGGAATCAAGACTTGGGAGTGGAAGTGGTAATAGTCGGGAGCACTATCAGCCAGAGGATTCTCCATTGCTTGCTCATCCAGGAGATCTGGTTATTTGCAAGAAGAAGAGAAAAGACAGGGAAAAGACGGGGGTAAAGACTAGGAATGGACCAGCTGGCCCTGTTTCACCCCCTAGCATGGGTCGCAGTATTAGAAGTCCTGGGCCAAATTCAGTCTCAAGGGAGACAAGATCAACCCAACAGGCATCACATTCACAAGGGTGGGCCAACCAGCCTTCGCAGCCTACTCAGCCTGCAAACAGGGGTACGGGGTCTGTTGGTTGGGCAAATCCGGTAAAGAGGTTAAGGACGGATTCTGGAAAGAGAAGGCCAAGCCATTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000229 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0005737 GO:0006091 GO:0008150 GO:0008152 GO:0009295 GO:0009507 GO:0009508 GO:0009521 GO:0009522 GO:0009526 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009719 GO:0009725 GO:0009737 GO:0009767 GO:0009773 GO:0009941 GO:0009987 GO:0010033 GO:0015979 GO:0016020 GO:0019684 GO:0019904 GO:0022900 GO:0030093 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0032991 GO:0033993 GO:0034357 GO:0042221 GO:0042646 GO:0042651 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0055035 GO:0055114 GO:0097159 GO:0097305 GO:0098796 GO:0098807 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CAAD

Protein Analysis

2271

Amino Acids

254.56

Weight (kDa)

8.76

Isoelectric Point (pI)

58.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
QLQ	PF08880	496 - 529	5.5e-08	QLQ
ResIII	PF04851	1024 - 1184	2.5e-08	Type III restriction enzyme, res subunit
SNF2-rel_dom	PF00176	1028 - 1332	6.9e-84	SNF2-related domain
DEAD	PF00270	1045 - 1188	2.3e-07	DEAD/DEAH box helicase
Helicase_C	PF00271	1353 - 1466	6.1e-23	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013322)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46820 AT2G46820
fragaria_vesca	FvH4_7g17800
malus_domestica	MD01G1089400.v1.1 MD07G1160900.v1.1
prunus_persica	Prupe.2G198900_v2.0.a1 Prupe.2G198900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0361201
rosa_laevigata	RLG00000027739
rosa_multiflora	Rmu_sc0010379.1_g000010 Rmu_sc0011132.1_g000003
rosa_roxburghii	Rroxscaffold_4G00295270
rosa_rugosa	Rorug01G0287500
rosa_samantha	Rh1AG298400 Rh1BG262300 Rh1CG279700 Rh1DG292500
rosa_wichuraiana	Rw1G026400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	4328
Acc36I	ACCTGC	1 cut(s)	1009
AccB1I	GGYRCC	2 cut(s)	1465, 3872
AccBSI	CCGCTC	1 cut(s)	6070
AccI	GTMKAC	3 cut(s)	119, 4969, 5713
AcoI	YGGCCR	1 cut(s)	607
AcsI	RAATTY	8 cut(s)	76, 568, 584, 1327, 1623, 5719, 6001, 6640
AcvI	CACGTG	1 cut(s)	3500
AcyI	GRCGYC	1 cut(s)	122
AflII	CTTAAG	2 cut(s)	3073, 3814
AflIII	ACRYGT	1 cut(s)	5790
AhdI	GACNNNNNGTC	1 cut(s)	559
AhlI	ACTAGT	2 cut(s)	2726, 4841
AjnI	CCWGG	9 cut(s)	609, 769, 895, 1480, 1630, 4068, 6173, 6511, 6631
AleI	CACNNNNGTG	1 cut(s)	6691
Alw21I	GWGCWC	4 cut(s)	876, 6099, 6253, 6477
Alw26I	GTCTC	9 cut(s)	1307, 2267, 3072, 3304, 3692, 5555, 6316, 6650, 6652
Alw44I	GTGCAC	1 cut(s)	6249
AlwNI	CAGNNNCTG	7 cut(s)	752, 1097, 1501, 2051, 2876, 2893, 5810
Ama87I	CYCGRG	2 cut(s)	32, 43
ApaI	GGGCCC	1 cut(s)	33
ApaLI	GTGCAC	1 cut(s)	6249
ApoI	RAATTY	8 cut(s)	76, 568, 584, 1327, 1623, 5719, 6001, 6640
ArsI	GACNNNNNNTTYG	2 cut(s)	2183, 2215
Asp700I	GAANNNNTTC	4 cut(s)	252, 1575, 2796, 3489
AspA2I	CCTAGG	3 cut(s)	344, 1226, 1367
AspLEI	GCGC	4 cut(s)	3365, 3531, 3788, 6063
AsuC2I	CCSGG	6 cut(s)	33, 34, 44, 45, 318, 6303
AsuHPI	GGTGA	6 cut(s)	693, 1113, 1480, 1562, 5651, 6591
AsuII	TTCGAA	1 cut(s)	3976
AsuNHI	GCTAGC	1 cut(s)	2464
AvaI	CYCGRG	2 cut(s)	32, 43
AvrII	CCTAGG	3 cut(s)	344, 1226, 1367
AxyI	CCTNAGG	2 cut(s)	1461, 5248
BaeGI	GKGCMC	2 cut(s)	33, 6253
BalI	TGGCCA	1 cut(s)	609
BamHI	GGATCC	2 cut(s)	4065, 4077
BanI	GGYRCC	2 cut(s)	1465, 3872
BanII	GRGCYC	4 cut(s)	33, 3099, 4387, 6099
BauI	CACGAG	2 cut(s)	669, 6098
BbrPI	CACGTG	1 cut(s)	3500
BbsI	GAAGAC	2 cut(s)	987, 3900
Bbv12I	GWGCWC	4 cut(s)	876, 6099, 6253, 6477
BceAI	ACGGC	4 cut(s)	79, 1219, 3542, 3733
BcgI	CGANNNNNNTGC	6 cut(s)	650, 684, 1361, 1395, 3966, 4000
BciT130I	CCWGG	9 cut(s)	611, 771, 897, 1482, 1632, 4070, 6175, 6513, 6633
BclI	TGATCA	5 cut(s)	2380, 4768, 4816, 5815, 6055
BcnI	CCSGG	6 cut(s)	33, 34, 44, 45, 318, 6303
BcoDI	GTCTC	9 cut(s)	1307, 2267, 3072, 3304, 3692, 5555, 6316, 6650, 6652
BcuI	ACTAGT	2 cut(s)	2726, 4841
BfmI	CTRYAG	8 cut(s)	753, 1613, 1872, 1944, 2868, 4230, 5802, 5821
BfoI	RGCGCY	1 cut(s)	3532
BfrI	CTTAAG	2 cut(s)	3073, 3814
BfuAI	ACCTGC	1 cut(s)	1009
BglI	GCCNNNNNGGC	2 cut(s)	1134, 2416
BglII	AGATCT	3 cut(s)	2302, 3801, 6517
BlnI	CCTAGG	3 cut(s)	344, 1226, 1367
BmeRI	GACNNNNNGTC	1 cut(s)	559
BmeT110I	CYCGRG	2 cut(s)	32, 43
BmrI	ACTGGG	2 cut(s)	6263, 6351
BmtI	GCTAGC	1 cut(s)	2468
BmuI	ACTGGG	2 cut(s)	6263, 6351
BoxI	GACNNNNGTC	1 cut(s)	6357
BpiI	GAAGAC	2 cut(s)	987, 3900
BplI	GAGNNNNNCTC	4 cut(s)	1039, 1071, 4615, 4647
BpmI	CTGGAG	9 cut(s)	987, 1653, 2952, 4295, 4503, 5040, 5118, 5421, 5915
Bpu10I	CCTNAGC	4 cut(s)	1138, 2777, 3758, 3905
Bpu14I	TTCGAA	1 cut(s)	3976
BpuEI	CTTGAG	7 cut(s)	890, 1017, 1551, 3336, 3716, 5607, 6634
BpuMI	CCSGG	6 cut(s)	33, 34, 44, 45, 318, 6303
Bsa29I	ATCGAT	1 cut(s)	4525
BsaAI	YACGTR	1 cut(s)	3500
BsaBI	GATNNNNATC	2 cut(s)	3513, 4344
BsaHI	GRCGYC	1 cut(s)	122
BsaI	GGTCTC	2 cut(s)	1307, 2267
BsaWI	WCCGGW	3 cut(s)	3673, 5669, 6763
Bse118I	RCCGGY	1 cut(s)	55
Bse21I	CCTNAGG	2 cut(s)	1461, 5248
Bse3DI	GCAATG	8 cut(s)	209, 498, 1349, 2130, 2412, 3551, 4712, 6497
Bse8I	GATNNNNATC	2 cut(s)	3513, 4344
BseBI	CCWGG	9 cut(s)	611, 771, 897, 1482, 1632, 4070, 6175, 6513, 6633
BseCI	ATCGAT	1 cut(s)	4525
BseJI	GATNNNNATC	2 cut(s)	3513, 4344
BseMI	GCAATG	8 cut(s)	209, 498, 1349, 2130, 2412, 3551, 4712, 6497
BseRI	GAGGAG	8 cut(s)	30, 33, 36, 1020, 1044, 1449, 4814, 5950
BseSI	GKGCMC	2 cut(s)	33, 6253
BseYI	CCCAGC	3 cut(s)	741, 2031, 4607
BsgI	GTGCAG	5 cut(s)	167, 2498, 2565, 2862, 4308
Bsh1285I	CGRYCG	1 cut(s)	563
BshNI	GGYRCC	2 cut(s)	1465, 3872
BshVI	ATCGAT	1 cut(s)	4525
BsiEI	CGRYCG	1 cut(s)	563
BsiHKAI	GWGCWC	4 cut(s)	876, 6099, 6253, 6477
BsiHKCI	CYCGRG	2 cut(s)	32, 43
BsiSI	CCGG	8 cut(s)	33, 44, 56, 318, 3674, 5670, 6302, 6764
BslFI	GGGAC	3 cut(s)	75, 5875, 6396
BsmAI	GTCTC	9 cut(s)	1307, 2267, 3072, 3304, 3692, 5555, 6316, 6650, 6652
BsmFI	GGGAC	3 cut(s)	75, 5875, 6396
BsmI	GAATGC	1 cut(s)	2977
Bso31I	GGTCTC	2 cut(s)	1307, 2267
BsoBI	CYCGRG	2 cut(s)	32, 43
Bsp119I	TTCGAA	1 cut(s)	3976
Bsp120I	GGGCCC	1 cut(s)	29
Bsp1286I	GDGCHC	7 cut(s)	33, 876, 3099, 4387, 6099, 6253, 6477
Bsp1407I	TGTACA	3 cut(s)	985, 3172, 4182
BspDI	ATCGAT	1 cut(s)	4525
BspHI	TCATGA	4 cut(s)	780, 2608, 4633, 5443
BspMAI	CTGCAG	2 cut(s)	2872, 5806
BspMI	ACCTGC	1 cut(s)	1009
BspOI	GCTAGC	1 cut(s)	2468
BspQI	GCTCTTC	2 cut(s)	258, 4632
BspT104I	TTCGAA	1 cut(s)	3976
BspT107I	GGYRCC	2 cut(s)	1465, 3872
BspTI	CTTAAG	2 cut(s)	3073, 3814
BspTNI	GGTCTC	2 cut(s)	1307, 2267
BsrBI	CCGCTC	1 cut(s)	6070
BsrDI	GCAATG	8 cut(s)	209, 498, 1349, 2130, 2412, 3551, 4712, 6497
BsrFI	RCCGGY	1 cut(s)	55
BsrGI	TGTACA	3 cut(s)	985, 3172, 4182
BssAI	RCCGGY	1 cut(s)	55
BssNI	GRCGYC	1 cut(s)	122
BssSI	CACGAG	2 cut(s)	669, 6098
BssT1I	CCWWGG	7 cut(s)	280, 344, 1226, 1297, 1367, 3936, 4974
Bst2BI	CACGAG	2 cut(s)	669, 6098
Bst2UI	CCWGG	9 cut(s)	611, 771, 897, 1482, 1632, 4070, 6175, 6513, 6633
BstACI	GRCGYC	1 cut(s)	122
BstAFI	CTTAAG	2 cut(s)	3073, 3814
BstAUI	TGTACA	3 cut(s)	985, 3172, 4182
BstBAI	YACGTR	1 cut(s)	3500
BstBI	TTCGAA	1 cut(s)	3976
BstH2I	RGCGCY	1 cut(s)	3532
BstHHI	GCGC	4 cut(s)	3365, 3531, 3788, 6063
BstMAI	GTCTC	9 cut(s)	1307, 2267, 3072, 3304, 3692, 5555, 6316, 6650, 6652
BstMCI	CGRYCG	1 cut(s)	563
BstNI	CCWGG	9 cut(s)	611, 771, 897, 1482, 1632, 4070, 6175, 6513, 6633
BstNSI	RCATGY	8 cut(s)	559, 814, 1508, 1682, 1808, 3023, 3284, 5794
BstPAI	GACNNNNGTC	1 cut(s)	6357
BstSFI	CTRYAG	8 cut(s)	753, 1613, 1872, 1944, 2868, 4230, 5802, 5821
BstSLI	GKGCMC	2 cut(s)	33, 6253
BstV2I	GAAGAC	2 cut(s)	987, 3900
BstX2I	RGATCY	8 cut(s)	1155, 2302, 3801, 4046, 4065, 4077, 5345, 6517
BstXI	CCANNNNNNTGG	2 cut(s)	913, 4394
BstYI	RGATCY	8 cut(s)	1155, 2302, 3801, 4046, 4065, 4077, 5345, 6517
Bsu15I	ATCGAT	1 cut(s)	4525
Bsu36I	CCTNAGG	2 cut(s)	1461, 5248
BsuTUI	ATCGAT	1 cut(s)	4525
BtsI	GCAGTG	1 cut(s)	4172
BtsIMutI	CAGTG	7 cut(s)	1699, 1734, 2072, 2554, 4172, 4488, 6225
BveI	ACCTGC	1 cut(s)	1009
CaiI	CAGNNNCTG	7 cut(s)	752, 1097, 1501, 2051, 2876, 2893, 5810
CciI	TCATGA	4 cut(s)	780, 2608, 4633, 5443
CfoI	GCGC	4 cut(s)	3365, 3531, 3788, 6063
Cfr10I	RCCGGY	1 cut(s)	55
Cfr9I	CCCGGG	2 cut(s)	32, 43
ClaI	ATCGAT	1 cut(s)	4525
CseI	GACGC	2 cut(s)	130, 2438
DraI	TTTAAA	1 cut(s)	3588
DrdI	GACNNNNNNGTC	1 cut(s)	4328
DriI	GACNNNNNGTC	1 cut(s)	559
DseDI	GACNNNNNNGTC	1 cut(s)	4328
EaeI	YGGCCR	1 cut(s)	607
Eam1105I	GACNNNNNGTC	1 cut(s)	559
EciI	GGCGGA	1 cut(s)	52
Ecl136II	GAGCTC	1 cut(s)	6097
Eco130I	CCWWGG	7 cut(s)	280, 344, 1226, 1297, 1367, 3936, 4974
Eco147I	AGGCCT	3 cut(s)	2419, 2483, 5539
Eco24I	GRGCYC	4 cut(s)	33, 3099, 4387, 6099
Eco31I	GGTCTC	2 cut(s)	1307, 2267
Eco32I	GATATC	3 cut(s)	3515, 5060, 6202
Eco53kI	GAGCTC	1 cut(s)	6097
Eco72I	CACGTG	1 cut(s)	3500
Eco81I	CCTNAGG	2 cut(s)	1461, 5248
Eco88I	CYCGRG	2 cut(s)	32, 43
EcoICRI	GAGCTC	1 cut(s)	6097
EcoO109I	RGGNCCY	3 cut(s)	530, 5954, 6383
EcoRI	GAATTC	2 cut(s)	76, 584
EcoRII	CCWGG	9 cut(s)	609, 769, 895, 1480, 1630, 4068, 6173, 6511, 6631
EcoRV	GATATC	3 cut(s)	3515, 5060, 6202
EcoT14I	CCWWGG	7 cut(s)	280, 344, 1226, 1297, 1367, 3936, 4974
EcoT22I	ATGCAT	1 cut(s)	5070
EcoT38I	GRGCYC	4 cut(s)	33, 3099, 4387, 6099
ErhI	CCWWGG	7 cut(s)	280, 344, 1226, 1297, 1367, 3936, 4974
FaqI	GGGAC	3 cut(s)	75, 5875, 6396
FauI	CCCGC	3 cut(s)	52, 2017, 3759
FauNDI	CATATG	3 cut(s)	2179, 5659, 6423
FbaI	TGATCA	5 cut(s)	2380, 4768, 4816, 5815, 6055
FblI	GTMKAC	3 cut(s)	119, 4969, 5713
FriOI	GRGCYC	4 cut(s)	33, 3099, 4387, 6099
GlaI	GCGC	4 cut(s)	3364, 3530, 3787, 6062
GsaI	CCCAGC	3 cut(s)	745, 2035, 4611
GsuI	CTGGAG	9 cut(s)	987, 1653, 2952, 4295, 4503, 5040, 5118, 5421, 5915
HaeII	RGCGCY	1 cut(s)	3532
HapII	CCGG	8 cut(s)	33, 44, 56, 318, 3674, 5670, 6302, 6764
HgaI	GACGC	2 cut(s)	130, 2438
HhaI	GCGC	4 cut(s)	3365, 3531, 3788, 6063
Hin1I	GRCGYC	1 cut(s)	122
Hin6I	GCGC	4 cut(s)	3363, 3529, 3786, 6061
HinP1I	GCGC	4 cut(s)	3363, 3529, 3786, 6061
HincII	GTYRAC	6 cut(s)	120, 412, 2998, 4441, 4970, 5714
HindII	GTYRAC	6 cut(s)	120, 412, 2998, 4441, 4970, 5714
HindIII	AAGCTT	2 cut(s)	441, 2804
HpaII	CCGG	8 cut(s)	33, 44, 56, 318, 3674, 5670, 6302, 6764
HphI	GGTGA	6 cut(s)	693, 1113, 1480, 1562, 5651, 6591
Hpy99I	CGWCG	9 cut(s)	76, 85, 106, 109, 112, 124, 4647, 5260, 5718
HpyCH4IV	ACGT	4 cut(s)	71, 1906, 2362, 3499
HpySE526I	ACGT	4 cut(s)	71, 1906, 2362, 3499
Hsp92I	GRCGYC	1 cut(s)	122
HspAI	GCGC	4 cut(s)	3363, 3529, 3786, 6061
KroI	GCCGGC	1 cut(s)	55
KroNI	GCCGGC	1 cut(s)	57
Ksp22I	TGATCA	5 cut(s)	2380, 4768, 4816, 5815, 6055
LguI	GCTCTTC	2 cut(s)	258, 4632
MaeII	ACGT	4 cut(s)	71, 1906, 2362, 3499
MbiI	CCGCTC	1 cut(s)	6070
MfeI	CAATTG	2 cut(s)	191, 4539
MflI	RGATCY	8 cut(s)	1155, 2302, 3801, 4046, 4065, 4077, 5345, 6517
MhlI	GDGCHC	7 cut(s)	33, 876, 3099, 4387, 6099, 6253, 6477
MlsI	TGGCCA	1 cut(s)	609
MluNI	TGGCCA	1 cut(s)	609
MlyI	GAGTC	6 cut(s)	409, 1697, 2102, 3723, 4645, 4937
MmeI	TCCRAC	7 cut(s)	1869, 2365, 5194, 5249, 5417, 5599, 6289
Mox20I	TGGCCA	1 cut(s)	609
Mph1103I	ATGCAT	1 cut(s)	5070
MroNI	GCCGGC	1 cut(s)	55
MroXI	GAANNNNTTC	4 cut(s)	252, 1575, 2796, 3489
MscI	TGGCCA	1 cut(s)	609
MslI	CAYNNNNRTG	7 cut(s)	329, 359, 1181, 4424, 4674, 5448, 6691
Msp20I	TGGCCA	1 cut(s)	609
MspA1I	CMGCKG	9 cut(s)	605, 752, 922, 1501, 2825, 2873, 2921, 4292, 6587
MspCI	CTTAAG	2 cut(s)	3073, 3814
MspI	CCGG	8 cut(s)	33, 44, 56, 318, 3674, 5670, 6302, 6764
MunI	CAATTG	2 cut(s)	191, 4539
Mva1269I	GAATGC	1 cut(s)	2977
MvaI	CCWGG	9 cut(s)	611, 771, 897, 1482, 1632, 4070, 6175, 6513, 6633
NaeI	GCCGGC	1 cut(s)	57
NciI	CCSGG	6 cut(s)	33, 34, 44, 45, 318, 6303
NdeI	CATATG	3 cut(s)	2179, 5659, 6423
NgoMIV	GCCGGC	1 cut(s)	55
NheI	GCTAGC	1 cut(s)	2464
NmuCI	GTSAC	6 cut(s)	699, 1199, 3385, 3579, 5450, 6274
NsiI	ATGCAT	1 cut(s)	5070
NspI	RCATGY	8 cut(s)	559, 814, 1508, 1682, 1808, 3023, 3284, 5794
NspV	TTCGAA	1 cut(s)	3976
OliI	CACNNNNGTG	1 cut(s)	6691
PaeI	GCATGC	2 cut(s)	814, 3284
PagI	TCATGA	4 cut(s)	780, 2608, 4633, 5443
PceI	AGGCCT	3 cut(s)	2419, 2483, 5539
PciI	ACATGT	1 cut(s)	5790
PciSI	GCTCTTC	2 cut(s)	258, 4632
PcsI	WCGNNNNNNNCGW	4 cut(s)	77, 98, 116, 2572
PctI	GAATGC	1 cut(s)	2977
PdiI	GCCGGC	1 cut(s)	57
PdmI	GAANNNNTTC	4 cut(s)	252, 1575, 2796, 3489
PfoI	TCCNGGA	3 cut(s)	1630, 4068, 6511
PleI	GAGTC	6 cut(s)	408, 1696, 2102, 3723, 4645, 4936
PmaCI	CACGTG	1 cut(s)	3500
PmlI	CACGTG	1 cut(s)	3500
PpsI	GAGTC	6 cut(s)	408, 1696, 2102, 3723, 4645, 4936
Ppu21I	YACGTR	1 cut(s)	3500
PpuMI	RGGWCCY	2 cut(s)	5954, 6383
PscI	ACATGT	1 cut(s)	5790
PshAI	GACNNNNGTC	1 cut(s)	6357
Psp124BI	GAGCTC	1 cut(s)	6099
Psp5II	RGGWCCY	2 cut(s)	5954, 6383
Psp6I	CCWGG	9 cut(s)	609, 769, 895, 1480, 1630, 4068, 6173, 6511, 6631
PspCI	CACGTG	1 cut(s)	3500
PspFI	CCCAGC	3 cut(s)	741, 2031, 4607
PspGI	CCWGG	9 cut(s)	609, 769, 895, 1480, 1630, 4068, 6173, 6511, 6631
PspOMI	GGGCCC	1 cut(s)	29
PspPPI	RGGWCCY	2 cut(s)	5954, 6383
PstI	CTGCAG	2 cut(s)	2872, 5806
PstNI	CAGNNNCTG	7 cut(s)	752, 1097, 1501, 2051, 2876, 2893, 5810
PsuI	RGATCY	8 cut(s)	1155, 2302, 3801, 4046, 4065, 4077, 5345, 6517
PvuII	CAGCTG	9 cut(s)	605, 752, 922, 1501, 2825, 2873, 2921, 4292, 6587
RseI	CAYNNNNRTG	7 cut(s)	329, 359, 1181, 4424, 4674, 5448, 6691
SacI	GAGCTC	1 cut(s)	6099
SalI	GTCGAC	3 cut(s)	118, 4968, 5712
SapI	GCTCTTC	2 cut(s)	258, 4632
SchI	GAGTC	6 cut(s)	409, 1697, 2102, 3723, 4645, 4937
SduI	GDGCHC	7 cut(s)	33, 876, 3099, 4387, 6099, 6253, 6477
SfcI	CTRYAG	8 cut(s)	753, 1613, 1872, 1944, 2868, 4230, 5802, 5821
SfuI	TTCGAA	1 cut(s)	3976
SmaI	CCCGGG	2 cut(s)	34, 45
SmiMI	CAYNNNNRTG	7 cut(s)	329, 359, 1181, 4424, 4674, 5448, 6691
SmlI	CTYRAG	9 cut(s)	869, 1032, 1566, 3073, 3351, 3695, 3814, 5622, 6649
SmoI	CTYRAG	9 cut(s)	869, 1032, 1566, 3073, 3351, 3695, 3814, 5622, 6649
SpeI	ACTAGT	2 cut(s)	2726, 4841
SphI	GCATGC	2 cut(s)	814, 3284
SrfI	GCCCGGGC	1 cut(s)	34
SseBI	AGGCCT	3 cut(s)	2419, 2483, 5539
SspI	AATATT	8 cut(s)	880, 1642, 3141, 3741, 4564, 4918, 5027, 6140
SstI	GAGCTC	1 cut(s)	6099
StuI	AGGCCT	3 cut(s)	2419, 2483, 5539
StyI	CCWWGG	7 cut(s)	280, 344, 1226, 1297, 1367, 3936, 4974
TaiI	ACGT	4 cut(s)	74, 1909, 2365, 3502
TaqII	GACCGA	1 cut(s)	705
TatI	WGTACW	5 cut(s)	985, 3172, 4182, 4844, 6181
TauI	GCSGC	2 cut(s)	2317, 3766
TscAI	CASTG	7 cut(s)	1699, 1734, 2079, 2561, 4172, 4495, 6232
TseFI	GTSAC	6 cut(s)	699, 1199, 3385, 3579, 5450, 6274
Tsp45I	GTSAC	6 cut(s)	699, 1199, 3385, 3579, 5450, 6274
TspGWI	ACGGA	4 cut(s)	2983, 3076, 3478, 6797
TspMI	CCCGGG	2 cut(s)	32, 43
TspRI	CASTG	7 cut(s)	1699, 1734, 2079, 2561, 4172, 4495, 6232
Vha464I	CTTAAG	2 cut(s)	3073, 3814
VneI	GTGCAC	1 cut(s)	6249
XapI	RAATTY	8 cut(s)	76, 568, 584, 1327, 1623, 5719, 6001, 6640
XbaI	TCTAGA	2 cut(s)	200, 1600
XceI	RCATGY	8 cut(s)	559, 814, 1508, 1682, 1808, 3023, 3284, 5794
XmaI	CCCGGG	2 cut(s)	32, 43
XmaJI	CCTAGG	3 cut(s)	344, 1226, 1367
XmiI	GTMKAC	3 cut(s)	119, 4969, 5713
XmnI	GAANNNNTTC	4 cut(s)	252, 1575, 2796, 3489
Zsp2I	ATGCAT	1 cut(s)	5070

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.