Rorug02G0015000

NADPH adrenodoxin oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005068
Physical Location & Seq
Forward (+)
1261614 .. 1262489
876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0015000.1

Sequence Viewer

Length: 876 bp
ATGGCCACACCAAACACCGCCGTCACCACCACCCCCACCCCCACCGTCGCATTCACCACTCCGCCAATCTACGCCGCCCGCCTCCGCCACGTCCTCACCCTCAACAACTTCACTCCCCTCTCCTCTCCCACACTCACCGTCCACCACACCCCCTCCACCGTCTCCGCCCTCAAACCCCACCTCTCGCCTCCTTCCCTAAACCTCTTCTCCGCCGTCGCCTTCCCTTCCCGCACCGCCATCGCCGCCTTCTCCGCCGCCGCAGACGACGTCGCTCATCCTCTCCTCTCCCCAAACGGCGCCGTCTTCACCGTCGCCGCGCTCGGCAAGGACGCCGAGCTCATTGACGACGGCTTCGTCCGCAAGCTCAGTCGAGATCGTCGCCGTGTCAGAACTCTGGTACCTCCGACCGCCACGCCGAGCGCGTTGGTTCACGCGCTCGGCGACGGCGGCGGCAGGAGGGTGCTGTGTCCGGTACCGGTGGTGTTGGGACTGACGGAGCCTCCGGTGGTGCCGGATTTTCTCCGGGAGCTCGGAGAAAGCGGTTGGGCTCCGGTTCGGGTCAATGCGTACGAGACGAGGTGGGCCGGGCCGGAGTGTGCGGCCCAATTGGTGGAGAGAATCAAGAACGGGGCTTTGGATGCGATCGTGTTTACTAGCACTGCGGAGGTGGAGGGGTTACTGAAGAGCTTCAAGGAGTTTGGGCTGGGCTGGGAGAGTGTCAAGAAATTGTGCCCGAAACTGCTCGTGGCGGCCCACGGGCCCGTTACTGCCTCCGGAGCCCGAAACCTTGGTGTCGGAGTAGATTTGGTGAGTTCTAAGTTCGACAGTTTTCAAGGTGTTGTTGATTCTTTGCGTTCTGAGATTTTCAAAGCTTAG

Protein Analysis

291

Amino Acids

30.71

Weight (kDa)

9.07

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEM4 PF02602 35 - 275 3.3e-18 Uroporphyrinogen-III synthase HemD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 353
AatII GACGTC 1 cut(s) 270
Acc65I GGTACC 2 cut(s) 397, 472
AccB1I GGYRCC 4 cut(s) 296, 397, 472, 508
AccB7I CCANNNNNTGG 1 cut(s) 610
AccII CGCG 3 cut(s) 317, 422, 434
AccIII TCCGGA 1 cut(s) 773
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 701
AcyI GRCGYC 3 cut(s) 267, 297, 330
AfaI GTAC 3 cut(s) 399, 474, 569
AfiI CCNNNNNNNGG 1 cut(s) 610
AgeI ACCGGT 1 cut(s) 475
AgsI TTSAA 3 cut(s) 691, 833, 868
AjiI CACGTC 1 cut(s) 91
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
AluBI AGCT 5 cut(s) 337, 364, 529, 687, 872
AluI AGCT 5 cut(s) 337, 364, 529, 687, 872
Alw21I GWGCWC 2 cut(s) 339, 531
Alw26I GTCTC 2 cut(s) 166, 566
Aor13HI TCCGGA 1 cut(s) 773
AoxI GGCC 6 cut(s) 3, 582, 587, 600, 750, 758
ApaI GGGCCC 1 cut(s) 762
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
AsiGI ACCGGT 1 cut(s) 475
Asp700I GAANNNNTTC 1 cut(s) 686
Asp718I GGTACC 2 cut(s) 397, 472
AspLEI GCGC 4 cut(s) 299, 319, 422, 436
AspS9I GGNCC 6 cut(s) 582, 587, 601, 751, 758, 759
AsuC2I CCSGG 2 cut(s) 524, 586
AsuHPI GGTGA 6 cut(s) 16, 46, 88, 127, 298, 820
BaeGI GKGCMC 2 cut(s) 734, 762
BaeI ACNNNNGTAYC 2 cut(s) 381, 414
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 4 cut(s) 296, 397, 472, 508
BanII GRGCYC 5 cut(s) 339, 531, 550, 762, 781
BauI CACGAG 1 cut(s) 743
BbsI GAAGAC 1 cut(s) 295
Bbv12I GWGCWC 2 cut(s) 339, 531
BccI CCATC 1 cut(s) 245
BceAI ACGGC 7 cut(s) 5, 197, 284, 310, 364, 366, 460
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BcnI CCSGG 2 cut(s) 524, 586
BcoDI GTCTC 2 cut(s) 166, 566
BfaI CTAG 1 cut(s) 654
BfoI RGCGCY 1 cut(s) 300
BisI GCNGC 9 cut(s) 75, 243, 255, 258, 315, 448, 451, 600, 750
BlsI GCNGC 9 cut(s) 76, 244, 256, 259, 316, 449, 452, 601, 751
Bme1390I CCNGG 2 cut(s) 524, 586
BmgBI CACGTC 1 cut(s) 91
BmgT120I GGNCC 6 cut(s) 582, 587, 601, 751, 758, 759
BmiI GGNNCC 8 cut(s) 298, 399, 474, 498, 510, 549, 760, 778
BmrFI CCNGG 2 cut(s) 524, 586
BmsI GCATC 1 cut(s) 628
BpiI GAAGAC 1 cut(s) 295
BpuMI CCSGG 2 cut(s) 524, 586
BsaHI GRCGYC 3 cut(s) 267, 297, 330
BsaJI CCNNGG 2 cut(s) 754, 787
BsaWI WCCGGW 5 cut(s) 469, 475, 502, 550, 773
BsaXI ACNNNNNCTCC 6 cut(s) 137, 167, 191, 221, 484, 514
Bsc4I CCNNNNNNNGG 1 cut(s) 610
Bse118I RCCGGY 1 cut(s) 475
BseAI TCCGGA 1 cut(s) 773
BseDI CCNNGG 2 cut(s) 754, 787
BseGI GGATG 2 cut(s) 274, 643
BseLI CCNNNNNNNGG 1 cut(s) 610
BseMII CTCAG 2 cut(s) 379, 849
BseRI GAGGAG 2 cut(s) 112, 272
BseSI GKGCMC 2 cut(s) 734, 762
BseYI CCCAGC 2 cut(s) 703, 708
Bsh1236I CGCG 3 cut(s) 317, 422, 434
Bsh1285I CGRYCG 2 cut(s) 408, 645
BshFI GGCC 6 cut(s) 5, 584, 589, 602, 752, 760
BshNI GGYRCC 4 cut(s) 296, 397, 472, 508
BshTI ACCGGT 1 cut(s) 475
BsiEI CGRYCG 2 cut(s) 408, 645
BsiHKAI GWGCWC 2 cut(s) 339, 531
BsiSI CCGG 9 cut(s) 470, 476, 503, 512, 523, 551, 585, 590, 774
BsiWI CGTACG 1 cut(s) 567
BslFI GGGAC 1 cut(s) 501
BslI CCNNNNNNNGG 1 cut(s) 610
BsmAI GTCTC 2 cut(s) 166, 566
BsmBI CGTCTC 2 cut(s) 166, 566
BsmFI GGGAC 1 cut(s) 501
BsmI GAATGC 1 cut(s) 50
BsnI GGCC 6 cut(s) 5, 584, 589, 602, 752, 760
Bsp120I GGGCCC 1 cut(s) 758
Bsp1286I GDGCHC 6 cut(s) 339, 531, 550, 734, 762, 781
Bsp13I TCCGGA 1 cut(s) 773
Bsp143I GATC 2 cut(s) 373, 642
BspANI GGCC 6 cut(s) 5, 584, 589, 602, 752, 760
BspCNI CTCAG 2 cut(s) 378, 850
BspEI TCCGGA 1 cut(s) 773
BspFNI CGCG 3 cut(s) 317, 422, 434
BspLI GGNNCC 8 cut(s) 298, 399, 474, 498, 510, 549, 760, 778
BspQI GCTCTTC 1 cut(s) 677
BspT107I GGYRCC 4 cut(s) 296, 397, 472, 508
BsrFI RCCGGY 1 cut(s) 475
BssAI RCCGGY 1 cut(s) 475
BssECI CCNNGG 2 cut(s) 754, 787
BssMI GATC 2 cut(s) 373, 642
BssNI GRCGYC 3 cut(s) 267, 297, 330
BssSI CACGAG 1 cut(s) 743
BssT1I CCWWGG 1 cut(s) 787
Bst2BI CACGAG 1 cut(s) 743
Bst4CI ACNGT 5 cut(s) 46, 139, 160, 310, 827
Bst6I CTCTTC 2 cut(s) 209, 677
BstACI GRCGYC 3 cut(s) 267, 297, 330
BstC8I GCNNGC 2 cut(s) 79, 362
BstDEI CTNAG 4 cut(s) 365, 816, 858, 873
BstDSI CCRYGG 1 cut(s) 754
BstF5I GGATG 2 cut(s) 274, 643
BstFNI CGCG 3 cut(s) 317, 422, 434
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 4 cut(s) 299, 319, 422, 436
BstKTI GATC 2 cut(s) 376, 645
BstMAI GTCTC 2 cut(s) 166, 566
BstMBI GATC 2 cut(s) 373, 642
BstMCI CGRYCG 2 cut(s) 408, 645
BstMWI GCNNNNNNNGC 6 cut(s) 242, 251, 357, 447, 638, 776
BstSCI CCNGG 2 cut(s) 522, 584
BstSLI GKGCMC 2 cut(s) 734, 762
BstUI CGCG 3 cut(s) 317, 422, 434
BstV2I GAAGAC 1 cut(s) 295
BsuRI GGCC 6 cut(s) 5, 584, 589, 602, 752, 760
BtgI CCRYGG 1 cut(s) 754
BtgZI GCGATG 1 cut(s) 223
BtrI CACGTC 1 cut(s) 91
BtsCI GGATG 2 cut(s) 274, 643
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 1 cut(s) 657
Cac8I GCNNGC 2 cut(s) 79, 362
CfoI GCGC 4 cut(s) 299, 319, 422, 436
Cfr10I RCCGGY 1 cut(s) 475
Cfr13I GGNCC 6 cut(s) 582, 587, 601, 751, 758, 759
CseI GACGC 1 cut(s) 338
Csp6I GTAC 3 cut(s) 398, 473, 568
CspAI ACCGGT 1 cut(s) 475
CviQI GTAC 3 cut(s) 398, 473, 568
DdeI CTNAG 4 cut(s) 365, 816, 858, 873
DinI GGCGCC 1 cut(s) 298
DpnI GATC 2 cut(s) 375, 644
DpnII GATC 2 cut(s) 373, 642
DrdI GACNNNNNNGTC 1 cut(s) 353
DseDI GACNNNNNNGTC 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 209, 677
EarI CTCTTC 2 cut(s) 209, 677
EciI GGCGGA 5 cut(s) 51, 74, 154, 199, 241
Ecl136II GAGCTC 2 cut(s) 337, 529
Eco130I CCWWGG 1 cut(s) 787
Eco24I GRGCYC 5 cut(s) 339, 531, 550, 762, 781
Eco53kI GAGCTC 2 cut(s) 337, 529
Eco57I CTGAAG 1 cut(s) 701
EcoICRI GAGCTC 2 cut(s) 337, 529
EcoT14I CCWWGG 1 cut(s) 787
EcoT38I GRGCYC 5 cut(s) 339, 531, 550, 762, 781
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
ErhI CCWWGG 1 cut(s) 787
Esp3I CGTCTC 2 cut(s) 166, 566
FaqI GGGAC 1 cut(s) 501
FauI CCCGC 2 cut(s) 86, 236
Fnu4HI GCNGC 9 cut(s) 75, 243, 255, 258, 315, 448, 451, 600, 750
FokI GGATG 2 cut(s) 261, 650
FriOI GRGCYC 5 cut(s) 339, 531, 550, 762, 781
Fsp4HI GCNGC 9 cut(s) 75, 243, 255, 258, 315, 448, 451, 600, 750
FspBI CTAG 1 cut(s) 654
GlaI GCGC 4 cut(s) 298, 318, 421, 435
GluI GCNGC 9 cut(s) 75, 243, 255, 258, 315, 448, 451, 600, 750
GsaI CCCAGC 2 cut(s) 707, 712
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 6 cut(s) 5, 584, 589, 602, 752, 760
HapII CCGG 9 cut(s) 470, 476, 503, 512, 523, 551, 585, 590, 774
HgaI GACGC 1 cut(s) 338
HhaI GCGC 4 cut(s) 299, 319, 422, 436
Hin1I GRCGYC 3 cut(s) 267, 297, 330
Hin6I GCGC 4 cut(s) 297, 317, 420, 434
HinP1I GCGC 4 cut(s) 297, 317, 420, 434
HindIII AAGCTT 1 cut(s) 870
HinfI GANTC 2 cut(s) 618, 845
HpaII CCGG 9 cut(s) 470, 476, 503, 512, 523, 551, 585, 590, 774
HphI GGTGA 6 cut(s) 16, 46, 88, 127, 298, 820
Hpy166II GTNNAC 3 cut(s) 142, 430, 651
Hpy188I TCNGA 5 cut(s) 389, 405, 533, 797, 859
Hpy188III TCNNGA 4 cut(s) 371, 622, 721, 774
Hpy8I GTNNAC 3 cut(s) 142, 430, 651
Hpy99I CGWCG 8 cut(s) 50, 218, 269, 272, 314, 350, 381, 446
HpyAV CCTTC 4 cut(s) 201, 229, 234, 256
HpyCH4III ACNGT 5 cut(s) 46, 139, 160, 310, 827
HpyCH4IV ACGT 2 cut(s) 90, 267
HpyF10VI GCNNNNNNNGC 6 cut(s) 242, 251, 357, 447, 638, 776
HpyF3I CTNAG 4 cut(s) 365, 816, 858, 873
HpySE526I ACGT 2 cut(s) 90, 267
Hsp92I GRCGYC 3 cut(s) 267, 297, 330
HspAI GCGC 4 cut(s) 297, 317, 420, 434
KasI GGCGCC 1 cut(s) 296
Kpn2I TCCGGA 1 cut(s) 773
KpnI GGTACC 2 cut(s) 401, 476
Kzo9I GATC 2 cut(s) 373, 642
LguI GCTCTTC 1 cut(s) 677
LmnI GCTCC 4 cut(s) 496, 526, 553, 776
LweI GCATC 1 cut(s) 628
MaeI CTAG 1 cut(s) 654
MaeII ACGT 2 cut(s) 90, 267
MaeIII GTNAC 3 cut(s) 22, 675, 763
MalI GATC 2 cut(s) 375, 644
MboI GATC 2 cut(s) 373, 642
MboII GAAGA 3 cut(s) 196, 295, 694
MfeI CAATTG 1 cut(s) 605
MhlI GDGCHC 6 cut(s) 339, 531, 550, 734, 762, 781
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 605, 725
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 297
MmeI TCCRAC 2 cut(s) 428, 775
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 773
MroXI GAANNNNTTC 1 cut(s) 686
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 9 cut(s) 470, 476, 503, 512, 523, 551, 585, 590, 774
MspR9I CCNGG 2 cut(s) 524, 586
MunI CAATTG 1 cut(s) 605
Mva1269I GAATGC 1 cut(s) 50
MvnI CGCG 3 cut(s) 317, 422, 434
MwoI GCNNNNNNNGC 6 cut(s) 242, 251, 357, 447, 638, 776
NarI GGCGCC 1 cut(s) 297
NciI CCSGG 2 cut(s) 524, 586
NdeII GATC 2 cut(s) 373, 642
NlaIV GGNNCC 8 cut(s) 298, 399, 474, 498, 510, 549, 760, 778
NmeAIII GCCGAG 4 cut(s) 300, 358, 417, 441
NmuCI GTSAC 1 cut(s) 22
PciSI GCTCTTC 1 cut(s) 677
PcsI WCGNNNNNNNCGW 3 cut(s) 351, 419, 438
PctI GAATGC 1 cut(s) 50
PdmI GAANNNNTTC 1 cut(s) 686
PfeI GAWTC 2 cut(s) 618, 845
Pfl23II CGTACG 1 cut(s) 567
PflFI GACNNNGTC 1 cut(s) 266
PflMI CCANNNNNTGG 1 cut(s) 610
PfoI TCCNGGA 1 cut(s) 522
PinAI ACCGGT 1 cut(s) 475
PkrI GCNGC 9 cut(s) 76, 244, 256, 259, 316, 449, 452, 601, 751
Ple19I CGATCG 1 cut(s) 645
PluTI GGCGCC 1 cut(s) 300
Psp124BI GAGCTC 2 cut(s) 339, 531
PspFI CCCAGC 2 cut(s) 703, 708
PspLI CGTACG 1 cut(s) 567
PspN4I GGNNCC 8 cut(s) 298, 399, 474, 498, 510, 549, 760, 778
PspOMI GGGCCC 1 cut(s) 758
PspPI GGNCC 6 cut(s) 582, 587, 601, 751, 758, 759
PsyI GACNNNGTC 1 cut(s) 266
PvuI CGATCG 1 cut(s) 645
RsaI GTAC 3 cut(s) 399, 474, 569
RsaNI GTAC 3 cut(s) 398, 473, 568
SacI GAGCTC 2 cut(s) 339, 531
SapI GCTCTTC 1 cut(s) 677
SatI GCNGC 9 cut(s) 75, 243, 255, 258, 315, 448, 451, 600, 750
Sau3AI GATC 2 cut(s) 373, 642
Sau96I GGNCC 6 cut(s) 582, 587, 601, 751, 758, 759
ScrFI CCNGG 2 cut(s) 524, 586
SduI GDGCHC 6 cut(s) 339, 531, 550, 734, 762, 781
SfaNI GCATC 1 cut(s) 628
SfoI GGCGCC 1 cut(s) 298
Sse9I AATT 2 cut(s) 605, 725
SspDI GGCGCC 1 cut(s) 296
SspMI CTAG 1 cut(s) 654
SstI GAGCTC 2 cut(s) 339, 531
StyD4I CCNGG 2 cut(s) 522, 584
StyI CCWWGG 1 cut(s) 787
TaaI ACNGT 5 cut(s) 46, 139, 160, 310, 827
TaiI ACGT 2 cut(s) 93, 270
TaqI TCGA 2 cut(s) 370, 822
TasI AATT 2 cut(s) 605, 725
TauI GCSGC 9 cut(s) 77, 245, 257, 260, 317, 450, 453, 602, 752
TfiI GAWTC 2 cut(s) 618, 845
TscAI CASTG 1 cut(s) 664
TseFI GTSAC 1 cut(s) 22
Tsp45I GTSAC 1 cut(s) 22
TspGWI ACGGA 1 cut(s) 509
TspRI CASTG 1 cut(s) 664
Tth111I GACNNNGTC 1 cut(s) 266
Van91I CCANNNNNTGG 1 cut(s) 610
XmnI GAANNNNTTC 1 cut(s) 686
XspI CTAG 1 cut(s) 654
ZraI GACGTC 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.