Rorug02G0032700

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008743
Physical Location & Seq
Forward (+)
2566894 .. 2569370
2477 bp
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UTR
Exon/CDS
Intron
Rorug02G0032700.1

Sequence Viewer

Length: 612 bp
ATGGCAAGCGCTCTCTTGTCAGCTCCTTGTTTTCTCTCTCCAAAAAATAAAGAACTTGGTGTGTTTGTAAGCAGATTCAGGAGTCTTAATAATGGCAATCTATTACATCATAGTTTGCTGAGGAAGAAGTATGGTGGCTCACTTGTGGTGACCTCAGTGTTTGGAAGGAAAGTCAAGACCACCAGAGAGACTGTGATACCTGAGCCGGATTACCGGATTCCAATTGTGTTACTAGGTCTATCCGGTGGATTAGCCTATACAAACAATCTTTTACCAGCTGCGCCTGTTGGTCTCCTTGGATTACTCCTATTGTTTCAGACTACTAGAGTTAGATTTGTCTTCGATGAAGAGGCTCTGGAAGTAAAAGTAGGGGAGGAGCTTGAGAATTCGGGTGAAAATGTATTCGTGGGTGGTAAAAACCGTTGGAAATACTCATCATTTGTGAACTGGGAGCTTTGGTGGCCAAATTTCCCTATTCTGGTGTATTTCAAAGAGACGCAAACAAAACCTGAAGGGCAAGTGCATTTCTTTCCGGTGATATTTAATGGGAAGCAACTTTATGATATCATGGTGGAGAGAGCTGGCCCTTCCCAAACTAGTGGACCAAAATAA

Protein Analysis

203

Amino Acids

22.78

Weight (kDa)

9.46

Isoelectric Point (pI)

29.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3119 PF11317 66 - 193 7.5e-35 Protein of unknown function (DUF3119)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 461
AcsI RAATTY 2 cut(s) 385, 466
AcuI CTGAAG 1 cut(s) 531
AfeI AGCGCT 1 cut(s) 10
AfiI CCNNNNNNNGG 1 cut(s) 478
AgsI TTSAA 1 cut(s) 490
AhlI ACTAGT 1 cut(s) 596
AluBI AGCT 5 cut(s) 23, 278, 379, 454, 581
AluI AGCT 5 cut(s) 23, 278, 379, 454, 581
Alw26I GTCTC 3 cut(s) 182, 296, 488
Aor51HI AGCGCT 1 cut(s) 10
AoxI GGCC 2 cut(s) 461, 583
ApeKI GCWGC 1 cut(s) 278
ApoI RAATTY 2 cut(s) 385, 466
AspLEI GCGC 2 cut(s) 11, 283
AspS9I GGNCC 2 cut(s) 584, 602
AsuHPI GGTGA 3 cut(s) 160, 404, 547
AvaII GGWCC 1 cut(s) 602
BalI TGGCCA 1 cut(s) 463
BbsI GAAGAC 1 cut(s) 331
BbvCI CCTCAGC 1 cut(s) 119
BbvI GCAGC 1 cut(s) 265
BcoDI GTCTC 3 cut(s) 182, 296, 488
BcuI ACTAGT 1 cut(s) 596
BfaI CTAG 3 cut(s) 233, 324, 597
BfoI RGCGCY 1 cut(s) 12
BisI GCNGC 1 cut(s) 279
BlsI GCNGC 1 cut(s) 280
Bme18I GGWCC 1 cut(s) 602
BmgT120I GGNCC 2 cut(s) 584, 602
BmrI ACTGGG 1 cut(s) 457
BmuI ACTGGG 1 cut(s) 457
BpiI GAAGAC 1 cut(s) 331
Bpu10I CCTNAGC 2 cut(s) 119, 201
BpuEI CTTGAG 1 cut(s) 401
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 295
BsaWI WCCGGW 3 cut(s) 213, 242, 532
BsaXI ACNNNNNCTCC 2 cut(s) 443, 473
Bsc4I CCNNNNNNNGG 1 cut(s) 478
Bse1I ACTGG 1 cut(s) 452
BseDI CCNNGG 1 cut(s) 295
BseLI CCNNNNNNNGG 1 cut(s) 478
BseMII CTCAG 3 cut(s) 110, 168, 192
BseNI ACTGG 1 cut(s) 452
BseRI GAGGAG 1 cut(s) 389
BseXI GCAGC 1 cut(s) 265
BshFI GGCC 2 cut(s) 463, 585
BsiSI CCGG 4 cut(s) 206, 214, 243, 533
BslI CCNNNNNNNGG 1 cut(s) 478
BsmAI GTCTC 3 cut(s) 182, 296, 488
BsmBI CGTCTC 1 cut(s) 488
BsnI GGCC 2 cut(s) 463, 585
Bso31I GGTCTC 1 cut(s) 296
BspANI GGCC 2 cut(s) 463, 585
BspCNI CTCAG 3 cut(s) 111, 167, 193
BspTNI GGTCTC 1 cut(s) 296
BsrI ACTGG 1 cut(s) 452
BssECI CCNNGG 1 cut(s) 295
BssT1I CCWWGG 1 cut(s) 295
Bst4CI ACNGT 2 cut(s) 193, 422
Bst6I CTCTTC 1 cut(s) 342
BstC8I GCNNGC 2 cut(s) 7, 583
BstDEI CTNAG 3 cut(s) 119, 154, 201
BstEII GGTNACC 1 cut(s) 148
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 2 cut(s) 11, 283
BstMAI GTCTC 3 cut(s) 182, 296, 488
BstMWI GCNNNNNNNGC 1 cut(s) 460
BstPI GGTNACC 1 cut(s) 148
BstV1I GCAGC 1 cut(s) 265
BstV2I GAAGAC 1 cut(s) 331
BstXI CCANNNNNNTGG 1 cut(s) 599
BsuRI GGCC 2 cut(s) 463, 585
BtsIMutI CAGTG 1 cut(s) 162
Cac8I GCNNGC 2 cut(s) 7, 583
CfoI GCGC 2 cut(s) 11, 283
Cfr13I GGNCC 2 cut(s) 584, 602
CseI GACGC 1 cut(s) 505
CviAII CATG 1 cut(s) 568
DdeI CTNAG 3 cut(s) 119, 154, 201
EaeI YGGCCR 1 cut(s) 461
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Eco130I CCWWGG 1 cut(s) 295
Eco31I GGTCTC 1 cut(s) 296
Eco32I GATATC 1 cut(s) 565
Eco47I GGWCC 1 cut(s) 602
Eco47III AGCGCT 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 531
Eco91I GGTNACC 1 cut(s) 148
EcoO65I GGTNACC 1 cut(s) 148
EcoRI GAATTC 1 cut(s) 385
EcoRV GATATC 1 cut(s) 565
EcoT14I CCWWGG 1 cut(s) 295
ErhI CCWWGG 1 cut(s) 295
Esp3I CGTCTC 1 cut(s) 488
FaeI CATG 1 cut(s) 571
FaiI YATR 5 cut(s) 111, 132, 258, 561, 569
FatI CATG 1 cut(s) 567
Fnu4HI GCNGC 1 cut(s) 279
Fsp4HI GCNGC 1 cut(s) 279
FspBI CTAG 3 cut(s) 233, 324, 597
GlaI GCGC 2 cut(s) 10, 282
GluI GCNGC 1 cut(s) 279
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 2 cut(s) 463, 585
HapII CCGG 4 cut(s) 206, 214, 243, 533
HgaI GACGC 1 cut(s) 505
HhaI GCGC 2 cut(s) 11, 283
Hin1II CATG 1 cut(s) 571
Hin6I GCGC 2 cut(s) 9, 281
HinP1I GCGC 2 cut(s) 9, 281
HinfI GANTC 3 cut(s) 75, 82, 217
HpaII CCGG 4 cut(s) 206, 214, 243, 533
HphI GGTGA 3 cut(s) 160, 404, 547
Hpy166II GTNNAC 2 cut(s) 445, 602
Hpy188I TCNGA 1 cut(s) 318
Hpy188III TCNNGA 3 cut(s) 79, 175, 356
Hpy8I GTNNAC 2 cut(s) 445, 602
HpyAV CCTTC 3 cut(s) 159, 506, 597
HpyCH4III ACNGT 2 cut(s) 193, 422
HpyCH4V TGCA 1 cut(s) 523
HpyF10VI GCNNNNNNNGC 1 cut(s) 460
HpyF3I CTNAG 3 cut(s) 119, 154, 201
Hsp92II CATG 1 cut(s) 571
HspAI GCGC 2 cut(s) 9, 281
LmnI GCTCC 3 cut(s) 28, 376, 451
Lsp1109I GCAGC 1 cut(s) 265
MaeI CTAG 3 cut(s) 233, 324, 597
MaeIII GTNAC 2 cut(s) 148, 228
MboII GAAGA 3 cut(s) 136, 331, 359
MfeI CAATTG 1 cut(s) 222
MlsI TGGCCA 1 cut(s) 463
MluCI AATT 3 cut(s) 222, 385, 466
MluNI TGGCCA 1 cut(s) 463
MlyI GAGTC 1 cut(s) 91
MmeI TCCRAC 1 cut(s) 404
MnlI CCTC 4 cut(s) 114, 163, 343, 367
Mox20I TGGCCA 1 cut(s) 463
MscI TGGCCA 1 cut(s) 463
MseI TTAA 2 cut(s) 87, 543
Msp20I TGGCCA 1 cut(s) 463
MspA1I CMGCKG 1 cut(s) 278
MspI CCGG 4 cut(s) 206, 214, 243, 533
MunI CAATTG 1 cut(s) 222
MwoI GCNNNNNNNGC 1 cut(s) 460
NlaIII CATG 1 cut(s) 571
NmuCI GTSAC 1 cut(s) 148
PfeI GAWTC 2 cut(s) 75, 217
PkrI GCNGC 1 cut(s) 280
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
PspEI GGTNACC 1 cut(s) 148
PspPI GGNCC 2 cut(s) 584, 602
PvuII CAGCTG 1 cut(s) 278
SaqAI TTAA 2 cut(s) 87, 543
SatI GCNGC 1 cut(s) 279
Sau96I GGNCC 2 cut(s) 584, 602
SchI GAGTC 1 cut(s) 91
SetI ASST 9 cut(s) 25, 155, 202, 238, 280, 381, 456, 511, 583
SinI GGWCC 1 cut(s) 602
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
SpeI ACTAGT 1 cut(s) 596
Sse9I AATT 3 cut(s) 222, 385, 466
SspMI CTAG 3 cut(s) 233, 324, 597
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 2 cut(s) 193, 422
TaqI TCGA 1 cut(s) 342
TasI AATT 3 cut(s) 222, 385, 466
TfiI GAWTC 2 cut(s) 75, 217
Tru1I TTAA 2 cut(s) 87, 543
Tru9I TTAA 2 cut(s) 87, 543
TscAI CASTG 1 cut(s) 162
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 1 cut(s) 278
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 1 cut(s) 360
TspRI CASTG 1 cut(s) 162
VpaK11BI GGWCC 1 cut(s) 602
XapI RAATTY 2 cut(s) 385, 466
XspI CTAG 3 cut(s) 233, 324, 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.