Rh2AG078000

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0008743
Physical Location & Seq
Forward (+)
6529800 .. 6530387
588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG078000.1

Sequence Viewer

Length: 588 bp
ATGAATGAGTATATAGCTCAGCTGATCAACCTGGCAGAGAAACTCTCTGAGCTCATGTGTGAGAATCTTGGTTTGGAGAAGGATGATGTCAAGGATGCATTTTCAGGTAGCAAGGGTCCTTCTGTCGGGACAAAAGTTGCAAAATATCCCAAGTGCTCTCAGCCAGAACGTGTGAGAGGATTGCGAGAGCACACTGATGCTGGTGGGATCATACTGTTGCTCCAAGACGACCAAGTTCCGGGTCTAGAATTCTTCAAAGATGGCAAATGGGTTGCAATTCCACCGTCTAAGAACAACACCATCTTTGTGAACACTGGTGACCAACTGGAAGTGCTGAGTAATGGAAGGTACAAAAGTACTTTGCACCGTGTCTTGACAGACAAGAATGGAAGTCGACTCTCCATTGCTACCTTCTATAATCCTGCCGGAGATGCCCTTATCTCTCCAGCTCCCAAACTCTTATACCCCAATAGCTTCCGTTTTCAAGATTATCTGAAGCTTTATGCCACCACAAAGTTCAATGACAAGGGCCCCAGATTCGAATCCATGAAACAAATGGCCAATGGAAACAATGGTTTCCATACCTAA

Protein Analysis

195

Amino Acids

21.71

Weight (kDa)

8.76

Isoelectric Point (pI)

22.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 47 - 141 1.8e-31 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 394
AclWI GGATC 1 cut(s) 215
AcoI YGGCCR 1 cut(s) 558
AcsI RAATTY 1 cut(s) 248
AcuI CTGAAG 1 cut(s) 515
AfaI GTAC 2 cut(s) 350, 358
AfiI CCNNNNNNNGG 2 cut(s) 125, 238
AflIII ACRYGT 1 cut(s) 169
AgsI TTSAA 3 cut(s) 256, 485, 520
AjnI CCWGG 1 cut(s) 30
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 6 cut(s) 17, 22, 52, 449, 474, 499
AluI AGCT 6 cut(s) 17, 22, 52, 449, 474, 499
Alw21I GWGCWC 3 cut(s) 54, 158, 192
AlwI GGATC 1 cut(s) 215
AoxI GGCC 2 cut(s) 529, 558
ApaI GGGCCC 1 cut(s) 533
ApoI RAATTY 1 cut(s) 248
AspS9I GGNCC 3 cut(s) 116, 529, 530
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 1 cut(s) 329
AsuII TTCGAA 1 cut(s) 540
AvaII GGWCC 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 533
BaeI ACNNNNGTAYC 2 cut(s) 340, 373
BalI TGGCCA 1 cut(s) 560
BanII GRGCYC 2 cut(s) 54, 533
Bbv12I GWGCWC 3 cut(s) 54, 158, 192
BccI CCATC 2 cut(s) 254, 308
BciT130I CCWGG 1 cut(s) 32
BclI TGATCA 1 cut(s) 24
BcnI CCSGG 1 cut(s) 240
BfaI CTAG 1 cut(s) 245
BlpI GCTNAGC 1 cut(s) 18
BmcAI AGTACT 1 cut(s) 358
Bme1390I CCNGG 2 cut(s) 32, 240
Bme18I GGWCC 1 cut(s) 116
BmgT120I GGNCC 3 cut(s) 116, 529, 530
BmiI GGNNCC 3 cut(s) 117, 531, 532
BmrFI CCNGG 2 cut(s) 32, 240
BmsI GCATC 3 cut(s) 85, 187, 421
BplI GAGNNNNNCTC 2 cut(s) 29, 61
BpmI CTGGAG 1 cut(s) 429
Bpu1102I GCTNAGC 1 cut(s) 18
Bpu14I TTCGAA 1 cut(s) 540
BpuMI CCSGG 1 cut(s) 240
BsaBI GATNNNNATC 1 cut(s) 541
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 238
Bse1I ACTGG 2 cut(s) 319, 330
Bse3DI GCAATG 1 cut(s) 402
Bse8I GATNNNNATC 1 cut(s) 541
BseBI CCWGG 1 cut(s) 32
BseGI GGATG 2 cut(s) 88, 100
BseJI GATNNNNATC 1 cut(s) 541
BseLI CCNNNNNNNGG 2 cut(s) 125, 238
BseMI GCAATG 1 cut(s) 402
BseMII CTCAG 4 cut(s) 32, 39, 173, 326
BseNI ACTGG 2 cut(s) 319, 330
BseSI GKGCMC 1 cut(s) 533
BshFI GGCC 2 cut(s) 531, 560
BsiHKAI GWGCWC 3 cut(s) 54, 158, 192
BsiSI CCGG 2 cut(s) 239, 426
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 2 cut(s) 125, 238
BsmFI GGGAC 1 cut(s) 142
BsnI GGCC 2 cut(s) 531, 560
Bsp119I TTCGAA 1 cut(s) 540
Bsp120I GGGCCC 1 cut(s) 529
Bsp1286I GDGCHC 4 cut(s) 54, 158, 192, 533
Bsp143I GATC 2 cut(s) 24, 207
Bsp1720I GCTNAGC 1 cut(s) 18
BspANI GGCC 2 cut(s) 531, 560
BspCNI CTCAG 4 cut(s) 31, 40, 172, 327
BspLI GGNNCC 3 cut(s) 117, 531, 532
BspPI GGATC 1 cut(s) 215
BspT104I TTCGAA 1 cut(s) 540
BsrDI GCAATG 1 cut(s) 402
BsrI ACTGG 2 cut(s) 319, 330
BssMI GATC 2 cut(s) 24, 207
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 3 cut(s) 216, 285, 368
BstBI TTCGAA 1 cut(s) 540
BstDEI CTNAG 5 cut(s) 18, 48, 159, 288, 335
BstEII GGTNACC 1 cut(s) 317
BstF5I GGATG 2 cut(s) 88, 100
BstKTI GATC 2 cut(s) 27, 210
BstMBI GATC 2 cut(s) 24, 207
BstMWI GCNNNNNNNGC 1 cut(s) 431
BstNI CCWGG 1 cut(s) 32
BstPI GGTNACC 1 cut(s) 317
BstSCI CCNGG 2 cut(s) 30, 238
BstSLI GKGCMC 1 cut(s) 533
BsuRI GGCC 2 cut(s) 531, 560
BtsCI GGATG 2 cut(s) 88, 100
BtsIMutI CAGTG 2 cut(s) 192, 312
Cfr13I GGNCC 3 cut(s) 116, 529, 530
Csp6I GTAC 2 cut(s) 349, 357
CviAII CATG 2 cut(s) 55, 547
CviJI RGCY 9 cut(s) 17, 22, 52, 163, 449, 474, 499, 531, 560
CviKI_1 RGCY 9 cut(s) 17, 22, 52, 163, 449, 474, 499, 531, 560
CviQI GTAC 2 cut(s) 349, 357
DdeI CTNAG 5 cut(s) 18, 48, 159, 288, 335
DpnI GATC 2 cut(s) 26, 209
DpnII GATC 2 cut(s) 24, 207
EaeI YGGCCR 1 cut(s) 558
Ecl136II GAGCTC 1 cut(s) 52
Eco24I GRGCYC 2 cut(s) 54, 533
Eco47I GGWCC 1 cut(s) 116
Eco53kI GAGCTC 1 cut(s) 52
Eco57I CTGAAG 1 cut(s) 515
Eco91I GGTNACC 1 cut(s) 317
EcoICRI GAGCTC 1 cut(s) 52
EcoO109I RGGNCCY 3 cut(s) 116, 529, 530
EcoO65I GGTNACC 1 cut(s) 317
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 30
EcoT22I ATGCAT 1 cut(s) 100
EcoT38I GRGCYC 2 cut(s) 54, 533
FaeI CATG 2 cut(s) 58, 550
FaiI YATR 9 cut(s) 12, 14, 56, 212, 417, 463, 504, 548, 582
FaqI GGGAC 1 cut(s) 142
FatI CATG 2 cut(s) 54, 546
FbaI TGATCA 1 cut(s) 24
FblI GTMKAC 1 cut(s) 394
FokI GGATG 2 cut(s) 95, 107
FriOI GRGCYC 2 cut(s) 54, 533
FspBI CTAG 1 cut(s) 245
GsuI CTGGAG 1 cut(s) 429
HaeIII GGCC 2 cut(s) 531, 560
HapII CCGG 2 cut(s) 239, 426
Hin1II CATG 2 cut(s) 58, 550
HincII GTYRAC 1 cut(s) 395
HindII GTYRAC 1 cut(s) 395
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 4 cut(s) 64, 396, 537, 542
HpaII CCGG 2 cut(s) 239, 426
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 2 cut(s) 310, 395
Hpy188I TCNGA 2 cut(s) 49, 495
Hpy188III TCNNGA 4 cut(s) 127, 245, 373, 485
Hpy8I GTNNAC 2 cut(s) 310, 395
HpyAV CCTTC 4 cut(s) 73, 129, 339, 421
HpyCH4III ACNGT 3 cut(s) 216, 285, 368
HpyCH4IV ACGT 1 cut(s) 169
HpyCH4V TGCA 4 cut(s) 98, 140, 275, 364
HpyF10VI GCNNNNNNNGC 1 cut(s) 431
HpyF3I CTNAG 5 cut(s) 18, 48, 159, 288, 335
HpySE526I ACGT 1 cut(s) 169
Hsp92II CATG 2 cut(s) 58, 550
Ksp22I TGATCA 1 cut(s) 24
Kzo9I GATC 2 cut(s) 24, 207
LmnI GCTCC 2 cut(s) 225, 454
LweI GCATC 3 cut(s) 85, 187, 421
MaeI CTAG 1 cut(s) 245
MaeII ACGT 1 cut(s) 169
MaeIII GTNAC 1 cut(s) 317
MalI GATC 2 cut(s) 26, 209
MboI GATC 2 cut(s) 24, 207
MboII GAAGA 1 cut(s) 244
MhlI GDGCHC 4 cut(s) 54, 158, 192, 533
MlsI TGGCCA 1 cut(s) 560
MluCI AATT 2 cut(s) 248, 276
MluNI TGGCCA 1 cut(s) 560
MlyI GAGTC 1 cut(s) 390
MnlI CCTC 1 cut(s) 170
Mox20I TGGCCA 1 cut(s) 560
Mph1103I ATGCAT 1 cut(s) 100
MscI TGGCCA 1 cut(s) 560
MslI CAYNNNNRTG 2 cut(s) 195, 305
Msp20I TGGCCA 1 cut(s) 560
MspA1I CMGCKG 1 cut(s) 22
MspI CCGG 2 cut(s) 239, 426
MspR9I CCNGG 2 cut(s) 32, 240
MvaI CCWGG 1 cut(s) 32
MwoI GCNNNNNNNGC 1 cut(s) 431
NciI CCSGG 1 cut(s) 240
NdeII GATC 2 cut(s) 24, 207
NlaIII CATG 2 cut(s) 58, 550
NlaIV GGNNCC 3 cut(s) 117, 531, 532
NmuCI GTSAC 1 cut(s) 317
NsiI ATGCAT 1 cut(s) 100
NspV TTCGAA 1 cut(s) 540
PfeI GAWTC 3 cut(s) 64, 537, 542
PleI GAGTC 1 cut(s) 390
PpsI GAGTC 1 cut(s) 390
PpuMI RGGWCCY 1 cut(s) 116
Psp124BI GAGCTC 1 cut(s) 54
Psp5II RGGWCCY 1 cut(s) 116
Psp6I CCWGG 1 cut(s) 30
PspEI GGTNACC 1 cut(s) 317
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 3 cut(s) 117, 531, 532
PspOMI GGGCCC 1 cut(s) 529
PspPI GGNCC 3 cut(s) 116, 529, 530
PspPPI RGGWCCY 1 cut(s) 116
PvuII CAGCTG 1 cut(s) 22
RsaI GTAC 2 cut(s) 350, 358
RsaNI GTAC 2 cut(s) 349, 357
RseI CAYNNNNRTG 2 cut(s) 195, 305
SacI GAGCTC 1 cut(s) 54
SalI GTCGAC 1 cut(s) 393
Sau3AI GATC 2 cut(s) 24, 207
Sau96I GGNCC 3 cut(s) 116, 529, 530
ScaI AGTACT 1 cut(s) 358
SchI GAGTC 1 cut(s) 390
ScrFI CCNGG 2 cut(s) 32, 240
SduI GDGCHC 4 cut(s) 54, 158, 192, 533
SfaNI GCATC 3 cut(s) 85, 187, 421
SfuI TTCGAA 1 cut(s) 540
SinI GGWCC 1 cut(s) 116
SmiMI CAYNNNNRTG 2 cut(s) 195, 305
Sse9I AATT 2 cut(s) 248, 276
SspMI CTAG 1 cut(s) 245
SstI GAGCTC 1 cut(s) 54
StyD4I CCNGG 2 cut(s) 30, 238
TaaI ACNGT 3 cut(s) 216, 285, 368
TaiI ACGT 1 cut(s) 172
TaqI TCGA 2 cut(s) 394, 540
TasI AATT 2 cut(s) 248, 276
TatI WGTACW 1 cut(s) 356
TfiI GAWTC 3 cut(s) 64, 537, 542
TscAI CASTG 2 cut(s) 199, 319
TseFI GTSAC 1 cut(s) 317
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 2 cut(s) 17, 563
TspGWI ACGGA 1 cut(s) 467
TspRI CASTG 2 cut(s) 199, 319
VpaK11BI GGWCC 1 cut(s) 116
XapI RAATTY 1 cut(s) 248
XbaI TCTAGA 1 cut(s) 244
XcmI CCANNNNNNNNNTGG 1 cut(s) 553
XmiI GTMKAC 1 cut(s) 394
XspI CTAG 1 cut(s) 245
ZrmI AGTACT 1 cut(s) 358
Zsp2I ATGCAT 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.