Rorug02G0114500

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0012662
Physical Location & Seq
Forward (+)
9775024 .. 9776202
1179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0114500.1

Sequence Viewer

Length: 1179 bp
ATGGACACCGTGAAATGGTCCGATCTTCCCAAGGAGCTCTGGCCGATCATCGGAAAATTCCTCCACAGCCGCATCGACGTTCTCCGCTTCCGCAGCGTCTGTTCCTTATGGCGTTCTTCTCGCCCTCCTTTCCAGCGACCTCTTCCTCCTCCTCCTCTCCCCCTCACGTTCTCTCCCTCCGGCGCCGGCGATCCCGAAAACCAAGCTCTACTTTTCCAAAGTACGACCTACCGCATGGAGTCAATCATCGACAAAAAACGCAGCGGTTCTTCACGATCAAAGCCGTGGTTGATGAAGCTTGAGCAGGACCCCAACTCCGGTAGAATGCGGCTAGTACATCCGGTGACTGGTTTTCTGTTCAGGTACTCCCCTAACGCTACTTCAAAGGAGTTCAGTTTATTGGAGTTTCGGGTTGTTGAGTTGCGAAAATCGTATGTGCTTAAGTTTGGGAAGAACAATGTTACTGTCAAGTCTGTGCAGAGATTGGTAGTCATGCCTTGTGACTTTTTGGACATTGATGATGTGTATGGGGTTTTTATGGTTTATAACGAAGGCAAGCTGGGTTTTGTGAGATTTGGAGATGAGAAGTTGACTCGTGTGGATGAACAGAATGCTCACTATGATGATATCATTGTGTATAAGGGTCAGTGCTATGTTGTTGATAAGTGGGGAACAATTTCATGGGTTAATTCAGCTTTGCAGGTGATTCAGTTTTCGCCTCCGCTATGTGGGTTTGGCGGGCGGAAGTATTTGGTGGAATGTCGTGATGATCTTTATGTGGTTGATCAGTTCTTTGAAAAGGTGATTCAGCAGAGCAATATAGTAGGGACCCCATTTCTGGATAATGAGTTACTTTGGTGGCATTATCATAAAATGCGTTCTGATGCAGAATCAATTGATTTCAAGGTTTATAAGCTGGATCAAGAATGGGGTAAATGGGTCGATGTGAAAGACATGGGAGATGACATCTTTATTTTAAGCAATGATGGATCTTTCTCTGTCTCCGCCAAAGAGTTTGCTGGAGTGAAGGGAAATTGCATTTTCTTTACTGAAAGTGCGTTAACTACTGGAAGGTTGTTCCCTTGTGTGTTCAATTTAGAGGATGGTTTGTTTGGCAATGCATCTTTGCGCTGTCCACAAATGGTCCATCCACCTTCAAGTTGGCTCAGCCCTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005798 GO:0005802 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0012505 GO:0012506 GO:0012510 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030641 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032588 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042470 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046961 GO:0048770 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

392

Amino Acids

45.06

Weight (kDa)

7.0

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 86 - 365 7.1e-36 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 546, 912
AarI CACCTGC 1 cut(s) 691
Acc36I ACCTGC 1 cut(s) 691
AccB1I GGYRCC 1 cut(s) 182
AclWI GGATC 3 cut(s) 185, 927, 997
AcoI YGGCCR 1 cut(s) 41
AcsI RAATTY 1 cut(s) 56
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 3 cut(s) 223, 336, 365
AfiI CCNNNNNNNGG 6 cut(s) 15, 50, 317, 347, 728, 838
AflII CTTAAG 1 cut(s) 440
AgsI TTSAA 5 cut(s) 384, 797, 904, 1093, 1158
AluBI AGCT 6 cut(s) 37, 206, 298, 559, 695, 916
AluI AGCT 6 cut(s) 37, 206, 298, 559, 695, 916
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 1006
AlwI GGATC 3 cut(s) 185, 927, 997
AlwNI CAGNNNCTG 1 cut(s) 99
AoxI GGCC 1 cut(s) 41
ApeKI GCWGC 2 cut(s) 93, 261
ApoI RAATTY 1 cut(s) 56
Asp700I GAANNNNTTC 1 cut(s) 676
AspLEI GCGC 2 cut(s) 185, 1131
AspS9I GGNCC 4 cut(s) 18, 307, 828, 1144
AsuHPI GGTGA 3 cut(s) 355, 715, 814
AvaII GGWCC 4 cut(s) 18, 307, 828, 1144
BanI GGYRCC 1 cut(s) 182
BanII GRGCYC 1 cut(s) 39
BauI CACGAG 1 cut(s) 594
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 2 cut(s) 105, 273
BccI CCATC 3 cut(s) 980, 1097, 1155
BceAI ACGGC 1 cut(s) 268
BclI TGATCA 1 cut(s) 784
BcoDI GTCTC 1 cut(s) 1006
BfaI CTAG 1 cut(s) 332
BfoI RGCGCY 1 cut(s) 186
BfrI CTTAAG 1 cut(s) 440
BfuAI ACCTGC 1 cut(s) 691
BisI GCNGC 4 cut(s) 70, 94, 262, 329
BlpI GCTNAGC 1 cut(s) 1166
BlsI GCNGC 4 cut(s) 71, 95, 263, 330
Bme18I GGWCC 4 cut(s) 18, 307, 828, 1144
BmgT120I GGNCC 4 cut(s) 18, 307, 828, 1144
BmiI GGNNCC 4 cut(s) 184, 309, 829, 830
BmsI GCATC 3 cut(s) 81, 874, 1130
BpmI CTGGAG 1 cut(s) 1041
Bpu1102I GCTNAGC 1 cut(s) 1166
BpuEI CTTGAG 1 cut(s) 320
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 2 cut(s) 30, 284
BsaWI WCCGGW 2 cut(s) 317, 340
BsaXI ACNNNNNCTCC 4 cut(s) 157, 187, 299, 329
Bsc4I CCNNNNNNNGG 6 cut(s) 15, 50, 317, 347, 728, 838
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 352, 1072
Bse3DI GCAATG 2 cut(s) 988, 1123
BseDI CCNNGG 2 cut(s) 30, 284
BseGI GGATG 4 cut(s) 337, 607, 1108, 1147
BseLI CCNNNNNNNGG 6 cut(s) 15, 50, 317, 347, 728, 838
BseMI GCAATG 2 cut(s) 988, 1123
BseNI ACTGG 2 cut(s) 352, 1072
BseRI GAGGAG 3 cut(s) 138, 141, 144
BseXI GCAGC 2 cut(s) 105, 273
BseYI CCCAGC 1 cut(s) 559
BsgI GTGCAG 1 cut(s) 497
BshFI GGCC 1 cut(s) 43
BshNI GGYRCC 1 cut(s) 182
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 4 cut(s) 180, 186, 318, 341
BslFI GGGAC 1 cut(s) 841
BslI CCNNNNNNNGG 6 cut(s) 15, 50, 317, 347, 728, 838
BsmAI GTCTC 1 cut(s) 1006
BsmFI GGGAC 1 cut(s) 841
BsmI GAATGC 2 cut(s) 330, 616
BsnI GGCC 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 8 cut(s) 22, 45, 190, 275, 769, 784, 919, 989
Bsp1720I GCTNAGC 1 cut(s) 1166
BspANI GGCC 1 cut(s) 43
BspCNI CTCAG 1 cut(s) 1179
BspLI GGNNCC 4 cut(s) 184, 309, 829, 830
BspMI ACCTGC 1 cut(s) 691
BspPI GGATC 3 cut(s) 185, 927, 997
BspT107I GGYRCC 1 cut(s) 182
BspTI CTTAAG 1 cut(s) 440
BsrDI GCAATG 2 cut(s) 988, 1123
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 2 cut(s) 352, 1072
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 2 cut(s) 30, 284
BssMI GATC 8 cut(s) 22, 45, 190, 275, 769, 784, 919, 989
BssNI GRCGYC 1 cut(s) 183
BssSI CACGAG 1 cut(s) 594
BssT1I CCWWGG 1 cut(s) 30
Bst2BI CACGAG 1 cut(s) 594
Bst4CI ACNGT 2 cut(s) 10, 466
Bst6I CTCTTC 1 cut(s) 147
BstACI GRCGYC 1 cut(s) 183
BstAFI CTTAAG 1 cut(s) 440
BstC8I GCNNGC 3 cut(s) 187, 557, 740
BstDEI CTNAG 1 cut(s) 1166
BstDSI CCRYGG 1 cut(s) 284
BstF5I GGATG 4 cut(s) 337, 607, 1108, 1147
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 2 cut(s) 185, 1131
BstKTI GATC 8 cut(s) 25, 48, 193, 278, 772, 787, 922, 992
BstMAI GTCTC 1 cut(s) 1006
BstMBI GATC 8 cut(s) 22, 45, 190, 275, 769, 784, 919, 989
BstMWI GCNNNNNNNGC 1 cut(s) 93
BstV1I GCAGC 2 cut(s) 105, 273
BstX2I RGATCY 1 cut(s) 989
BstYI RGATCY 1 cut(s) 989
BsuRI GGCC 1 cut(s) 43
BtgI CCRYGG 1 cut(s) 284
BtsCI GGATG 4 cut(s) 337, 607, 1108, 1147
BtsIMutI CAGTG 1 cut(s) 653
BveI ACCTGC 1 cut(s) 691
Cac8I GCNNGC 3 cut(s) 187, 557, 740
CaiI CAGNNNCTG 1 cut(s) 99
CfoI GCGC 2 cut(s) 185, 1131
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 4 cut(s) 18, 307, 828, 1144
CseI GACGC 1 cut(s) 85
Csp6I GTAC 3 cut(s) 222, 335, 364
CviAII CATG 4 cut(s) 235, 493, 681, 955
CviQI GTAC 3 cut(s) 222, 335, 364
DdeI CTNAG 1 cut(s) 1166
DinI GGCGCC 1 cut(s) 184
DpnI GATC 8 cut(s) 24, 47, 192, 277, 771, 786, 921, 991
DpnII GATC 8 cut(s) 22, 45, 190, 275, 769, 784, 919, 989
EaeI YGGCCR 1 cut(s) 41
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
EciI GGCGGA 2 cut(s) 757, 994
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 39
Eco32I GATATC 1 cut(s) 628
Eco47I GGWCC 4 cut(s) 18, 307, 828, 1144
Eco53kI GAGCTC 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 37
EcoO109I RGGNCCY 2 cut(s) 307, 828
EcoRV GATATC 1 cut(s) 628
EcoT14I CCWWGG 1 cut(s) 30
EcoT22I ATGCAT 1 cut(s) 1123
EcoT38I GRGCYC 1 cut(s) 39
EgeI GGCGCC 1 cut(s) 184
EheI GGCGCC 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 4 cut(s) 238, 496, 684, 958
FalI AAGNNNNNCTT 2 cut(s) 195, 227
FaqI GGGAC 1 cut(s) 841
FatI CATG 4 cut(s) 234, 492, 680, 954
FauI CCCGC 1 cut(s) 731
FbaI TGATCA 1 cut(s) 784
Fnu4HI GCNGC 4 cut(s) 70, 94, 262, 329
FokI GGATG 4 cut(s) 324, 614, 1115, 1134
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 4 cut(s) 70, 94, 262, 329
FspBI CTAG 1 cut(s) 332
GlaI GCGC 2 cut(s) 184, 1130
GluI GCNGC 4 cut(s) 70, 94, 262, 329
GsaI CCCAGC 1 cut(s) 563
GsuI CTGGAG 1 cut(s) 1041
HaeII RGCGCY 1 cut(s) 186
HaeIII GGCC 1 cut(s) 43
HapII CCGG 4 cut(s) 180, 186, 318, 341
HgaI GACGC 1 cut(s) 85
HhaI GCGC 2 cut(s) 185, 1131
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 4 cut(s) 238, 496, 684, 958
Hin6I GCGC 2 cut(s) 183, 1129
HinP1I GCGC 2 cut(s) 183, 1129
HincII GTYRAC 2 cut(s) 591, 1062
HindII GTYRAC 2 cut(s) 591, 1062
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 5 cut(s) 239, 592, 706, 805, 890
HpaI GTTAAC 1 cut(s) 1062
HpaII CCGG 4 cut(s) 180, 186, 318, 341
HphI GGTGA 3 cut(s) 355, 715, 814
Hpy166II GTNNAC 3 cut(s) 591, 1062, 1136
Hpy188I TCNGA 3 cut(s) 22, 53, 883
Hpy188III TCNNGA 5 cut(s) 194, 273, 764, 839, 923
Hpy8I GTNNAC 3 cut(s) 591, 1062, 1136
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 4 cut(s) 545, 1021, 1065, 1164
HpyCH4III ACNGT 2 cut(s) 10, 466
HpyCH4IV ACGT 2 cut(s) 78, 167
HpyCH4V TGCA 5 cut(s) 478, 700, 887, 1038, 1121
HpyF10VI GCNNNNNNNGC 1 cut(s) 93
HpyF3I CTNAG 1 cut(s) 1166
HpySE526I ACGT 2 cut(s) 78, 167
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 4 cut(s) 238, 496, 684, 958
HspAI GCGC 2 cut(s) 183, 1129
KasI GGCGCC 1 cut(s) 182
KflI GGGWCCC 1 cut(s) 828
KroI GCCGGC 1 cut(s) 185
KroNI GCCGGC 1 cut(s) 187
Ksp22I TGATCA 1 cut(s) 784
KspAI GTTAAC 1 cut(s) 1062
Kzo9I GATC 8 cut(s) 22, 45, 190, 275, 769, 784, 919, 989
LmnI GCTCC 1 cut(s) 34
Lsp1109I GCAGC 2 cut(s) 105, 273
LweI GCATC 3 cut(s) 81, 874, 1130
MaeI CTAG 1 cut(s) 332
MaeII ACGT 2 cut(s) 78, 167
MaeIII GTNAC 4 cut(s) 343, 460, 500, 849
MalI GATC 8 cut(s) 24, 47, 192, 277, 771, 786, 921, 991
MboI GATC 8 cut(s) 22, 45, 190, 275, 769, 784, 919, 989
MboII GAAGA 5 cut(s) 17, 108, 134, 261, 463
MfeI CAATTG 1 cut(s) 894
MflI RGATCY 1 cut(s) 989
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 6 cut(s) 56, 675, 688, 894, 1033, 1093
Mly113I GGCGCC 1 cut(s) 183
MlyI GAGTC 2 cut(s) 248, 586
Mph1103I ATGCAT 1 cut(s) 1123
MreI CGCCGGCG 1 cut(s) 185
MroNI GCCGGC 1 cut(s) 185
MroXI GAANNNNTTC 1 cut(s) 676
MseI TTAA 4 cut(s) 441, 687, 977, 1061
MslI CAYNNNNRTG 1 cut(s) 621
MspA1I CMGCKG 1 cut(s) 264
MspCI CTTAAG 1 cut(s) 440
MspI CCGG 4 cut(s) 180, 186, 318, 341
MunI CAATTG 1 cut(s) 894
Mva1269I GAATGC 2 cut(s) 330, 616
MwoI GCNNNNNNNGC 1 cut(s) 93
NaeI GCCGGC 1 cut(s) 187
NarI GGCGCC 1 cut(s) 183
NdeII GATC 8 cut(s) 22, 45, 190, 275, 769, 784, 919, 989
NgoMIV GCCGGC 1 cut(s) 185
NlaIII CATG 4 cut(s) 238, 496, 684, 958
NlaIV GGNNCC 4 cut(s) 184, 309, 829, 830
NmuCI GTSAC 2 cut(s) 343, 500
NsiI ATGCAT 1 cut(s) 1123
PaqCI CACCTGC 1 cut(s) 691
PctI GAATGC 2 cut(s) 330, 616
PdiI GCCGGC 1 cut(s) 187
PdmI GAANNNNTTC 1 cut(s) 676
PfeI GAWTC 3 cut(s) 706, 805, 890
PkrI GCNGC 4 cut(s) 71, 95, 263, 330
PleI GAGTC 2 cut(s) 247, 586
PluTI GGCGCC 1 cut(s) 186
PpsI GAGTC 2 cut(s) 247, 586
PpuMI RGGWCCY 2 cut(s) 307, 828
PsiI TTATAA 2 cut(s) 546, 912
Psp124BI GAGCTC 1 cut(s) 39
Psp5II RGGWCCY 2 cut(s) 307, 828
PspFI CCCAGC 1 cut(s) 559
PspN4I GGNNCC 4 cut(s) 184, 309, 829, 830
PspPI GGNCC 4 cut(s) 18, 307, 828, 1144
PspPPI RGGWCCY 2 cut(s) 307, 828
PstNI CAGNNNCTG 1 cut(s) 99
PsuI RGATCY 1 cut(s) 989
RsaI GTAC 3 cut(s) 223, 336, 365
RsaNI GTAC 3 cut(s) 222, 335, 364
RseI CAYNNNNRTG 1 cut(s) 621
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 4 cut(s) 441, 687, 977, 1061
SatI GCNGC 4 cut(s) 70, 94, 262, 329
Sau3AI GATC 8 cut(s) 22, 45, 190, 275, 769, 784, 919, 989
Sau96I GGNCC 4 cut(s) 18, 307, 828, 1144
SchI GAGTC 2 cut(s) 248, 586
SduI GDGCHC 1 cut(s) 39
SfaNI GCATC 3 cut(s) 81, 874, 1130
SfoI GGCGCC 1 cut(s) 184
SgrAI CRCCGGYG 1 cut(s) 185
SinI GGWCC 4 cut(s) 18, 307, 828, 1144
SmiMI CAYNNNNRTG 1 cut(s) 621
SmlI CTYRAG 2 cut(s) 299, 440
SmoI CTYRAG 2 cut(s) 299, 440
Sse9I AATT 6 cut(s) 56, 675, 688, 894, 1033, 1093
SspDI GGCGCC 1 cut(s) 182
SspMI CTAG 1 cut(s) 332
SstI GAGCTC 1 cut(s) 39
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 10, 466
TaiI ACGT 2 cut(s) 81, 170
TaqI TCGA 3 cut(s) 75, 249, 942
TasI AATT 6 cut(s) 56, 675, 688, 894, 1033, 1093
TatI WGTACW 1 cut(s) 334
TauI GCSGC 2 cut(s) 72, 331
TfiI GAWTC 3 cut(s) 706, 805, 890
Tru1I TTAA 4 cut(s) 441, 687, 977, 1061
Tru9I TTAA 4 cut(s) 441, 687, 977, 1061
TscAI CASTG 1 cut(s) 653
TseFI GTSAC 2 cut(s) 343, 500
TseI GCWGC 2 cut(s) 93, 261
Tsp45I GTSAC 2 cut(s) 343, 500
TspDTI ATGAA 3 cut(s) 308, 618, 669
TspRI CASTG 1 cut(s) 653
Vha464I CTTAAG 1 cut(s) 440
VpaK11BI GGWCC 4 cut(s) 18, 307, 828, 1144
XapI RAATTY 1 cut(s) 56
XcmI CCANNNNNNNNNTGG 1 cut(s) 1158
XmnI GAANNNNTTC 1 cut(s) 676
XspI CTAG 1 cut(s) 332
Zsp2I ATGCAT 1 cut(s) 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.