Rorug02G0115800

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0014639
Physical Location & Seq
Reverse (-)
9854056 .. 9856918
2863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0115800.1

Sequence Viewer

Length: 1116 bp
ATGGCTACCTTGGCGCTCCTGAGCCTAACATCTCCTTCACTCTCCTCTCCCAGATCTCTTCCTCGCTCAAATCACACCAGATTCGGTGTCCTTACTACCACTTCGGGTTGGGCTCGGAGAACTGTGCGGCTTCCCGTTCTATGCTCAGCTTCGGAGCCCAATCAAAACGCCGAATCCGAACCGGAAACGACGTCGTCTCCGCCGTCGTCTTCGAGTGACTTGACCTACAAACTATGTGCCGGGCTCGGAGGCATCGGGTTTCTCGAAACGACCTACTTGACGTTCCTCAAGCTCACGAATTCCGATGCCTTCTGCCCCACCGGCGGCGGCGGAAGTTGCGGCGACATCCTCAACAGTGAATACGCCGTCGTTTTTGGTGTTCCTCTTCCGTTGTTTGGAATGCTTGCATATGGGTTAGTTGCTACACTTGGTGTGCAGCTGTTGACTGCAAAGAGTTTACCTTTTGGAATTGGGAAATCTGATGCTAGCTTGGTTTTACTTGGGACTACAACCTCCATGGCAGCTGCTAGTGCTTGTTTCTTGTACATTCTTAGCACACAGTTTTCGGGAACTTCGTGTTCGTATTGTTTGTTATCGGCTGTCCTCTCCTTCAGCTTATTTTTCATCACTTTAAAGGATTTTGGGTTCGAAAAAGTACAAAAAGAGGCGGGTTTATTGGTGTGTATAGCTGTCTTGGTGGCTGTGACTCTGAACAGGTCATATAGTGCACCTGTATCCTCAAGCCCATCTGATGTTGATCTTCCATACTTTACAACTGAGATAACAACACCATCAAGTCCGTTTGCTATATCTCTTGCAAAGCATCTAAATGCTATAGGTGCTAAAATGTATGGCGCTTTTTGGTGTTCACATTGTGTTGAACAAAAACAGATGTTTGGACATGAAGCAGCGAAGGAATTGAACTATGTTGAGTGCTTCCCTGGTGGATTTAGAAAGGGAACTATAATGGCAAAGGCATGTATTGATGCCAAACTTGAAGGTTTTCCTACTTGGGTCATCAATGGACAGGTTTTGAGTGGAGAAAAAGATTTGCTAGAGCTTGCAGAGGCATCTGGGTTTGAGTTGAACGAATTTAGTCAGACCCTCATAGAGTAA

Protein Analysis

371

Amino Acids

39.35

Weight (kDa)

5.36

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VKOR PF07884 78 - 210 9.3e-26 Vitamin K epoxide reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 194
AciI CCGC 7 cut(s) 127, 200, 324, 327, 330, 339, 668
AcsI RAATTY 2 cut(s) 298, 1091
AcuI CTGAAG 1 cut(s) 595
AcyI GRCGYC 1 cut(s) 191
AdeI CACNNNGTG 2 cut(s) 431, 875
AfaI GTAC 2 cut(s) 545, 657
AfiI CCNNNNNNNGG 2 cut(s) 323, 395
AgsI TTSAA 4 cut(s) 881, 922, 998, 1087
AjnI CCWGG 1 cut(s) 940
AloI GAACNNNNNNTCC 2 cut(s) 629, 661
AluBI AGCT 8 cut(s) 149, 292, 439, 489, 524, 615, 689, 1060
AluI AGCT 8 cut(s) 149, 292, 439, 489, 524, 615, 689, 1060
Alw21I GWGCWC 1 cut(s) 730
Alw26I GTCTC 1 cut(s) 201
Alw44I GTGCAC 1 cut(s) 726
ApaLI GTGCAC 1 cut(s) 726
ApeKI GCWGC 4 cut(s) 436, 521, 524, 908
ApoI RAATTY 2 cut(s) 298, 1091
Asp700I GAANNNNTTC 1 cut(s) 1002
AspLEI GCGC 2 cut(s) 16, 857
AsuC2I CCSGG 1 cut(s) 241
AsuII TTCGAA 1 cut(s) 648
AsuNHI GCTAGC 1 cut(s) 485
BaeGI GKGCMC 1 cut(s) 730
BanII GRGCYC 3 cut(s) 115, 159, 246
BbsI GAAGAC 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 730
BbvI GCAGC 4 cut(s) 448, 511, 533, 920
BccI CCATC 2 cut(s) 754, 799
BceAI ACGGC 2 cut(s) 187, 350
BciT130I CCWGG 1 cut(s) 942
BciVI GTATCC 1 cut(s) 745
BcnI CCSGG 1 cut(s) 241
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 3 cut(s) 486, 528, 1055
BfmI CTRYAG 1 cut(s) 834
BfoI RGCGCY 2 cut(s) 17, 858
BfuI GTATCC 1 cut(s) 745
BglI GCCNNNNNGGC 1 cut(s) 321
BglII AGATCT 1 cut(s) 53
BisI GCNGC 8 cut(s) 128, 325, 328, 340, 437, 522, 525, 909
BlpI GCTNAGC 1 cut(s) 145
BlsI GCNGC 8 cut(s) 129, 326, 329, 341, 438, 523, 526, 910
Bme1390I CCNGG 2 cut(s) 241, 942
BmiI GGNNCC 1 cut(s) 156
BmrFI CCNGG 2 cut(s) 241, 942
BmsI GCATC 6 cut(s) 261, 295, 472, 832, 976, 1079
BmtI GCTAGC 1 cut(s) 489
BpiI GAAGAC 1 cut(s) 201
Bpu10I CCTNAGC 1 cut(s) 20
Bpu1102I GCTNAGC 1 cut(s) 145
Bpu14I TTCGAA 1 cut(s) 648
BpuEI CTTGAG 2 cut(s) 272, 724
BpuMI CCSGG 1 cut(s) 241
BsaBI GATNNNNATC 1 cut(s) 756
BsaHI GRCGYC 1 cut(s) 191
BsaJI CCNNGG 3 cut(s) 9, 516, 940
BsaWI WCCGGW 1 cut(s) 181
BsaXI ACNNNNNCTCC 2 cut(s) 181, 211
Bsc4I CCNNNNNNNGG 2 cut(s) 323, 395
Bse118I RCCGGY 1 cut(s) 320
Bse8I GATNNNNATC 1 cut(s) 756
BseBI CCWGG 1 cut(s) 942
BseDI CCNNGG 3 cut(s) 9, 516, 940
BseGI GGATG 1 cut(s) 345
BseJI GATNNNNATC 1 cut(s) 756
BseLI CCNNNNNNNGG 2 cut(s) 323, 395
BseMII CTCAG 3 cut(s) 11, 159, 768
BseRI GAGGAG 1 cut(s) 34
BseSI GKGCMC 1 cut(s) 730
BseXI GCAGC 4 cut(s) 448, 511, 533, 920
BsgI GTGCAG 1 cut(s) 455
BsiHKAI GWGCWC 1 cut(s) 730
BsiSI CCGG 3 cut(s) 182, 240, 321
BslFI GGGAC 1 cut(s) 517
BslI CCNNNNNNNGG 2 cut(s) 323, 395
BsmAI GTCTC 1 cut(s) 201
BsmBI CGTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 517
BsmI GAATGC 1 cut(s) 405
Bsp119I TTCGAA 1 cut(s) 648
Bsp1286I GDGCHC 4 cut(s) 115, 159, 246, 730
Bsp1407I TGTACA 1 cut(s) 543
Bsp143I GATC 2 cut(s) 53, 757
Bsp1720I GCTNAGC 1 cut(s) 145
Bsp19I CCATGG 1 cut(s) 516
BspACI CCGC 7 cut(s) 127, 200, 324, 327, 330, 339, 668
BspCNI CTCAG 3 cut(s) 12, 158, 769
BspLI GGNNCC 1 cut(s) 156
BspOI GCTAGC 1 cut(s) 489
BspT104I TTCGAA 1 cut(s) 648
BsrFI RCCGGY 1 cut(s) 320
BsrGI TGTACA 1 cut(s) 543
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 3 cut(s) 9, 516, 940
BssMI GATC 2 cut(s) 53, 757
BssNI GRCGYC 1 cut(s) 191
BssT1I CCWWGG 2 cut(s) 9, 516
Bst2UI CCWGG 1 cut(s) 942
Bst4CI ACNGT 3 cut(s) 124, 356, 561
Bst6I CTCTTC 2 cut(s) 63, 390
BstACI GRCGYC 1 cut(s) 191
BstAUI TGTACA 1 cut(s) 543
BstBI TTCGAA 1 cut(s) 648
BstC8I GCNNGC 3 cut(s) 405, 487, 1062
BstDEI CTNAG 4 cut(s) 20, 145, 551, 777
BstDSI CCRYGG 1 cut(s) 516
BstF5I GGATG 1 cut(s) 345
BstH2I RGCGCY 2 cut(s) 17, 858
BstHHI GCGC 2 cut(s) 16, 857
BstKTI GATC 2 cut(s) 56, 760
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 2 cut(s) 53, 757
BstMWI GCNNNNNNNGC 5 cut(s) 11, 321, 336, 530, 839
BstNI CCWGG 1 cut(s) 942
BstNSI RCATGY 1 cut(s) 981
BstSCI CCNGG 2 cut(s) 239, 940
BstSFI CTRYAG 1 cut(s) 834
BstSLI GKGCMC 1 cut(s) 730
BstV1I GCAGC 4 cut(s) 448, 511, 533, 920
BstV2I GAAGAC 1 cut(s) 201
BstX2I RGATCY 1 cut(s) 53
BstYI RGATCY 1 cut(s) 53
BsuI GTATCC 1 cut(s) 745
BtgI CCRYGG 1 cut(s) 516
BtsCI GGATG 1 cut(s) 345
BtsIMutI CAGTG 1 cut(s) 361
Cac8I GCNNGC 3 cut(s) 405, 487, 1062
CfoI GCGC 2 cut(s) 16, 857
Cfr10I RCCGGY 1 cut(s) 320
Csp6I GTAC 2 cut(s) 544, 656
CviAII CATG 3 cut(s) 517, 902, 978
CviQI GTAC 2 cut(s) 544, 656
DdeI CTNAG 4 cut(s) 20, 145, 551, 777
DpnI GATC 2 cut(s) 55, 759
DpnII GATC 2 cut(s) 53, 757
DraI TTTAAA 1 cut(s) 633
DraIII CACNNNGTG 2 cut(s) 431, 875
Eam1104I CTCTTC 2 cut(s) 63, 390
EarI CTCTTC 2 cut(s) 63, 390
EciI GGCGGA 2 cut(s) 189, 345
Eco130I CCWWGG 2 cut(s) 9, 516
Eco24I GRGCYC 3 cut(s) 115, 159, 246
Eco57I CTGAAG 1 cut(s) 595
EcoRI GAATTC 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 940
EcoT14I CCWWGG 2 cut(s) 9, 516
EcoT38I GRGCYC 3 cut(s) 115, 159, 246
ErhI CCWWGG 2 cut(s) 9, 516
Esp3I CGTCTC 1 cut(s) 201
FaeI CATG 3 cut(s) 520, 905, 981
FaqI GGGAC 1 cut(s) 517
FatI CATG 3 cut(s) 516, 901, 977
FauI CCCGC 1 cut(s) 661
FauNDI CATATG 1 cut(s) 409
Fnu4HI GCNGC 8 cut(s) 128, 325, 328, 340, 437, 522, 525, 909
FokI GGATG 1 cut(s) 332
FriOI GRGCYC 3 cut(s) 115, 159, 246
Fsp4HI GCNGC 8 cut(s) 128, 325, 328, 340, 437, 522, 525, 909
FspBI CTAG 3 cut(s) 486, 528, 1055
GlaI GCGC 2 cut(s) 15, 856
GluI GCNGC 8 cut(s) 128, 325, 328, 340, 437, 522, 525, 909
HaeII RGCGCY 2 cut(s) 17, 858
HapII CCGG 3 cut(s) 182, 240, 321
HhaI GCGC 2 cut(s) 16, 857
Hin1I GRCGYC 1 cut(s) 191
Hin1II CATG 3 cut(s) 520, 905, 981
Hin6I GCGC 2 cut(s) 14, 855
HinP1I GCGC 2 cut(s) 14, 855
HincII GTYRAC 1 cut(s) 444
HindII GTYRAC 1 cut(s) 444
HinfI GANTC 3 cut(s) 81, 173, 706
HpaII CCGG 3 cut(s) 182, 240, 321
Hpy166II GTNNAC 4 cut(s) 444, 458, 728, 869
Hpy188I TCNGA 9 cut(s) 117, 154, 178, 248, 304, 481, 711, 751, 1101
Hpy188III TCNNGA 4 cut(s) 19, 263, 295, 567
Hpy8I GTNNAC 4 cut(s) 444, 458, 728, 869
Hpy99I CGWCG 4 cut(s) 193, 196, 208, 371
HpyAV CCTTC 5 cut(s) 45, 319, 619, 907, 992
HpyCH4III ACNGT 3 cut(s) 124, 356, 561
HpyCH4IV ACGT 2 cut(s) 191, 281
HpyCH4V TGCA 6 cut(s) 407, 436, 449, 728, 818, 1064
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 321, 336, 530, 839
HpyF3I CTNAG 4 cut(s) 20, 145, 551, 777
HpySE526I ACGT 2 cut(s) 191, 281
Hsp92I GRCGYC 1 cut(s) 191
Hsp92II CATG 3 cut(s) 520, 905, 981
HspAI GCGC 2 cut(s) 14, 855
Kzo9I GATC 2 cut(s) 53, 757
LmnI GCTCC 2 cut(s) 21, 154
Lsp1109I GCAGC 4 cut(s) 448, 511, 533, 920
LweI GCATC 6 cut(s) 261, 295, 472, 832, 976, 1079
MaeI CTAG 3 cut(s) 486, 528, 1055
MaeII ACGT 2 cut(s) 191, 281
MaeIII GTNAC 2 cut(s) 215, 703
MalI GATC 2 cut(s) 55, 759
MboI GATC 2 cut(s) 53, 757
MboII GAAGA 4 cut(s) 50, 201, 377, 752
MflI RGATCY 1 cut(s) 53
MhlI GDGCHC 4 cut(s) 115, 159, 246, 730
MluCI AATT 4 cut(s) 298, 468, 917, 1091
MlyI GAGTC 1 cut(s) 700
MroXI GAANNNNTTC 1 cut(s) 1002
MseI TTAA 1 cut(s) 632
MslI CAYNNNNRTG 1 cut(s) 828
MspA1I CMGCKG 2 cut(s) 439, 524
MspI CCGG 3 cut(s) 182, 240, 321
MspR9I CCNGG 2 cut(s) 241, 942
Mva1269I GAATGC 1 cut(s) 405
MvaI CCWGG 1 cut(s) 942
MwoI GCNNNNNNNGC 5 cut(s) 11, 321, 336, 530, 839
NciI CCSGG 1 cut(s) 241
NcoI CCATGG 1 cut(s) 516
NdeI CATATG 1 cut(s) 409
NdeII GATC 2 cut(s) 53, 757
NheI GCTAGC 1 cut(s) 485
NlaIII CATG 3 cut(s) 520, 905, 981
NlaIV GGNNCC 1 cut(s) 156
NmuCI GTSAC 2 cut(s) 215, 703
NspI RCATGY 1 cut(s) 981
NspV TTCGAA 1 cut(s) 648
PcsI WCGNNNNNNNCGW 4 cut(s) 174, 200, 261, 572
PctI GAATGC 1 cut(s) 405
PdmI GAANNNNTTC 1 cut(s) 1002
PfeI GAWTC 2 cut(s) 81, 173
PflFI GACNNNGTC 1 cut(s) 193
PkrI GCNGC 8 cut(s) 129, 326, 329, 341, 438, 523, 526, 910
PleI GAGTC 1 cut(s) 700
PpsI GAGTC 1 cut(s) 700
Psp6I CCWGG 1 cut(s) 940
PspGI CCWGG 1 cut(s) 940
PspN4I GGNNCC 1 cut(s) 156
PsuI RGATCY 1 cut(s) 53
PsyI GACNNNGTC 1 cut(s) 193
PvuII CAGCTG 2 cut(s) 439, 524
RsaI GTAC 2 cut(s) 545, 657
RsaNI GTAC 2 cut(s) 544, 656
RseI CAYNNNNRTG 1 cut(s) 828
SaqAI TTAA 1 cut(s) 632
SatI GCNGC 8 cut(s) 128, 325, 328, 340, 437, 522, 525, 909
Sau3AI GATC 2 cut(s) 53, 757
SchI GAGTC 1 cut(s) 700
ScrFI CCNGG 2 cut(s) 241, 942
SduI GDGCHC 4 cut(s) 115, 159, 246, 730
SfaNI GCATC 6 cut(s) 261, 295, 472, 832, 976, 1079
SfcI CTRYAG 1 cut(s) 834
SfuI TTCGAA 1 cut(s) 648
SgrAI CRCCGGYG 1 cut(s) 320
SmiMI CAYNNNNRTG 1 cut(s) 828
SmlI CTYRAG 2 cut(s) 287, 739
SmoI CTYRAG 2 cut(s) 287, 739
Sse9I AATT 4 cut(s) 298, 468, 917, 1091
SsiI CCGC 7 cut(s) 127, 200, 324, 327, 330, 339, 668
SspMI CTAG 3 cut(s) 486, 528, 1055
StyD4I CCNGG 2 cut(s) 239, 940
StyI CCWWGG 2 cut(s) 9, 516
TaaI ACNGT 3 cut(s) 124, 356, 561
TaiI ACGT 2 cut(s) 194, 284
TaqI TCGA 3 cut(s) 212, 264, 648
TasI AATT 4 cut(s) 298, 468, 917, 1091
TatI WGTACW 2 cut(s) 543, 655
TauI GCSGC 4 cut(s) 130, 327, 330, 342
TfiI GAWTC 2 cut(s) 81, 173
Tru1I TTAA 1 cut(s) 632
Tru9I TTAA 1 cut(s) 632
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 2 cut(s) 215, 703
TseI GCWGC 4 cut(s) 436, 521, 524, 908
Tsp45I GTSAC 2 cut(s) 215, 703
TspDTI ATGAA 2 cut(s) 613, 918
TspGWI ACGGA 2 cut(s) 378, 789
TspRI CASTG 1 cut(s) 361
Tth111I GACNNNGTC 1 cut(s) 193
VneI GTGCAC 1 cut(s) 726
XapI RAATTY 2 cut(s) 298, 1091
XceI RCATGY 1 cut(s) 981
XmnI GAANNNNTTC 1 cut(s) 1002
XspI CTAG 3 cut(s) 486, 528, 1055
ZraI GACGTC 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.