Rh2BG172000

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014639
Physical Location & Seq
Reverse (-)
15142823 .. 15144011
1189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG172000.1

Sequence Viewer

Length: 438 bp
ATGGCTTCTCATGGCCGGAGGAATCGTGAAGGCTCTATCTTTCCAGTCACCATTCCAAGCTTCTTTGTGATAGTCTTGGCTCAAGCACTTGAAGATGGAAAACTTGAAATTCCAAGAACTGTTGTGAGGCAATGTGGAGATCGTCTGGCACAGATAATACTTATCAAGGTTTCTAAAGGAGCAGGCTGGGGAATCGAGTTGGCAAAGAGTAACAGAAACATTTGGCTGCAAAAGGGTTGGCCTGGATTCGCAAAGCATTACTCGCTGGAGCATGGTAGCTTACTCCTCTTCAGCTCCCAAGGGGATTACTTGCATTTCGGGGTACAGATTTTCAAGAGGAACACTTTGGAGATAAACTATCCACCTTTGGAGATGAATTATCCACCGAATTCTAGTAGCACTGTGGAGATGAAATCCTGCATCCAATTCAAGCCATGA

Protein Analysis

145

Amino Acids

16.36

Weight (kDa)

9.55

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 23 - 113 8.1e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 13
AcsI RAATTY 2 cut(s) 108, 388
AcuI CTGAAG 1 cut(s) 274
AfaI GTAC 1 cut(s) 324
AgsI TTSAA 4 cut(s) 92, 107, 334, 430
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 3 cut(s) 60, 279, 294
AluI AGCT 3 cut(s) 60, 279, 294
AoxI GGCC 2 cut(s) 13, 239
ApeKI GCWGC 1 cut(s) 226
ApoI RAATTY 2 cut(s) 108, 388
AsuHPI GGTGA 1 cut(s) 40
BbvI GCAGC 1 cut(s) 213
BccI CCATC 1 cut(s) 89
BciT130I CCWGG 1 cut(s) 243
BfaI CTAG 1 cut(s) 393
BisI GCNGC 1 cut(s) 227
BlsI GCNGC 1 cut(s) 228
Bme1390I CCNGG 1 cut(s) 243
BmrFI CCNGG 1 cut(s) 243
BmsI GCATC 1 cut(s) 429
BpmI CTGGAG 1 cut(s) 287
BpuEI CTTGAG 1 cut(s) 66
BsaJI CCNNGG 1 cut(s) 298
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bse1I ACTGG 1 cut(s) 44
Bse3DI GCAATG 1 cut(s) 137
BseBI CCWGG 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 298
BseGI GGATG 1 cut(s) 420
BseMI GCAATG 1 cut(s) 137
BseNI ACTGG 1 cut(s) 44
BseRI GAGGAG 1 cut(s) 275
BseXI GCAGC 1 cut(s) 213
BseYI CCCAGC 1 cut(s) 186
BshFI GGCC 2 cut(s) 15, 241
BsiSI CCGG 1 cut(s) 16
BsnI GGCC 2 cut(s) 15, 241
Bsp143I GATC 1 cut(s) 139
BspANI GGCC 2 cut(s) 15, 241
BsrDI GCAATG 1 cut(s) 137
BsrI ACTGG 1 cut(s) 44
BssECI CCNNGG 1 cut(s) 298
BssMI GATC 1 cut(s) 139
BssT1I CCWWGG 1 cut(s) 298
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 2 cut(s) 121, 403
Bst6I CTCTTC 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 184
BstF5I GGATG 1 cut(s) 420
BstKTI GATC 1 cut(s) 142
BstMBI GATC 1 cut(s) 139
BstMWI GCNNNNNNNGC 1 cut(s) 262
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 213
BsuRI GGCC 2 cut(s) 15, 241
BtsCI GGATG 1 cut(s) 420
BtsIMutI CAGTG 1 cut(s) 399
Cac8I GCNNGC 1 cut(s) 184
Csp6I GTAC 1 cut(s) 323
CviAII CATG 3 cut(s) 11, 272, 435
CviQI GTAC 1 cut(s) 323
DpnI GATC 1 cut(s) 141
DpnII GATC 1 cut(s) 139
EaeI YGGCCR 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Eco130I CCWWGG 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 274
EcoRI GAATTC 1 cut(s) 388
EcoRII CCWGG 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 298
ErhI CCWWGG 1 cut(s) 298
FaeI CATG 3 cut(s) 14, 275, 438
FaiI YATR 3 cut(s) 12, 273, 436
FatI CATG 3 cut(s) 10, 271, 434
Fnu4HI GCNGC 1 cut(s) 227
FokI GGATG 1 cut(s) 407
Fsp4HI GCNGC 1 cut(s) 227
FspBI CTAG 1 cut(s) 393
GluI GCNGC 1 cut(s) 227
GsaI CCCAGC 1 cut(s) 190
GsuI CTGGAG 1 cut(s) 287
HaeIII GGCC 2 cut(s) 15, 241
HapII CCGG 1 cut(s) 16
Hin1II CATG 3 cut(s) 14, 275, 438
HindIII AAGCTT 1 cut(s) 58
HinfI GANTC 3 cut(s) 22, 192, 246
HpaII CCGG 1 cut(s) 16
HphI GGTGA 1 cut(s) 40
Hpy188III TCNNGA 2 cut(s) 26, 334
HpyAV CCTTC 1 cut(s) 23
HpyCH4III ACNGT 2 cut(s) 121, 403
HpyCH4V TGCA 3 cut(s) 229, 313, 420
HpyF10VI GCNNNNNNNGC 1 cut(s) 262
Hsp92II CATG 3 cut(s) 14, 275, 438
Kzo9I GATC 1 cut(s) 139
LmnI GCTCC 3 cut(s) 179, 268, 299
LpnPI CCDG 9 cut(s) 29, 57, 131, 168, 172, 228, 251, 255, 430
Lsp1109I GCAGC 1 cut(s) 213
LweI GCATC 1 cut(s) 429
MaeI CTAG 1 cut(s) 393
MaeIII GTNAC 2 cut(s) 46, 209
MalI GATC 1 cut(s) 141
MboI GATC 1 cut(s) 139
MboII GAAGA 2 cut(s) 104, 280
MluCI AATT 4 cut(s) 108, 376, 388, 425
MnlI CCTC 4 cut(s) 12, 120, 296, 330
MspI CCGG 1 cut(s) 16
MspR9I CCNGG 1 cut(s) 243
MvaI CCWGG 1 cut(s) 243
MwoI GCNNNNNNNGC 1 cut(s) 262
NdeII GATC 1 cut(s) 139
NlaIII CATG 3 cut(s) 14, 275, 438
NmuCI GTSAC 1 cut(s) 46
PfeI GAWTC 3 cut(s) 22, 192, 246
PkrI GCNGC 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 241
PspFI CCCAGC 1 cut(s) 186
PspGI CCWGG 1 cut(s) 241
RsaI GTAC 1 cut(s) 324
RsaNI GTAC 1 cut(s) 323
SatI GCNGC 1 cut(s) 227
Sau3AI GATC 1 cut(s) 139
ScrFI CCNGG 1 cut(s) 243
SetI ASST 5 cut(s) 62, 171, 281, 296, 367
SfaNI GCATC 1 cut(s) 429
SmlI CTYRAG 1 cut(s) 81
SmoI CTYRAG 1 cut(s) 81
Sse9I AATT 4 cut(s) 108, 376, 388, 425
SspMI CTAG 1 cut(s) 393
StyD4I CCNGG 1 cut(s) 241
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 2 cut(s) 121, 403
TaqI TCGA 1 cut(s) 195
TasI AATT 4 cut(s) 108, 376, 388, 425
TfiI GAWTC 3 cut(s) 22, 192, 246
TscAI CASTG 1 cut(s) 406
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 1 cut(s) 226
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 2 cut(s) 389, 425
TspRI CASTG 1 cut(s) 406
XapI RAATTY 2 cut(s) 108, 388
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.