Rorug02G0182200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006271
Physical Location & Seq
Reverse (-)
16968062 .. 16968769
708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0182200.1

Sequence Viewer

Length: 708 bp
ATGCCGAATTTGAGTAAGAAGAAGTGCCAATGGAATGTCCAGCCAGCGACTAAGGAATATCGTGCCCAGTTACCATCAGACCCTAACCCTAGAAACTTGCAATACCGACCTAAACAGACACCTGTTGAGGAACTATACCGAAGATATAAGGATCCACATGAAGAGATGATCATGGCTGAGGGTATCAGTCGCCTCTGCACTGATCTCGATGTGGATCCCCAAGACATTGTCATGTTGGTTATGTCATGGCACATGAAGGCAGCCACCATGTGCGAGTTTTCAAAGCAAGAGTTCTTCAATGGACTTAAAGCCCTAAAGATTGATTCCATTGAGAAGTTCCGAAGCGCGCTACCGTCTATGAGATCTGAGCTGAAGGATAAGGACAAGTTTAAGGAGGTGTATAATTTTACTTTTGACTGGGCAAAGGAAAAGGGGCAGAAGTCTTTGCAGCTGGATACTGCAATTGGAATGTGGCAATTGCTGTTTGCCGAAATGACTCCGAAGTGGCCCTATGTTGATCAATGGTGTGAATTCTTGGAGGAGAGGCACAAGCTGGCAATACCTAAGGACACATGGAATCAACTATTGGATTTTGTAAGGAATGTGGATCCATCGCTGTCGAATTTTGATGAAGAAAGAGCTTGGCCCTGTCTTGTCGACGATTTTGTGGATTACTTGAAGGAGAAAGGCTTGGTGCAAACTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

235

Amino Acids

27.89

Weight (kDa)

5.57

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 113 - 225 8.6e-38 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 657
AccII CGCG 1 cut(s) 347
AclWI GGATC 6 cut(s) 146, 159, 209, 222, 602, 615
AcsI RAATTY 3 cut(s) 7, 530, 622
AcuI CTGAAG 1 cut(s) 392
AdeI CACNNNGTG 1 cut(s) 270
AgsI TTSAA 3 cut(s) 282, 298, 679
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
AluBI AGCT 4 cut(s) 370, 451, 553, 641
AluI AGCT 4 cut(s) 370, 451, 553, 641
AlwI GGATC 6 cut(s) 146, 159, 209, 222, 602, 615
AoxI GGCC 2 cut(s) 506, 644
ApeKI GCWGC 2 cut(s) 260, 448
ApoI RAATTY 3 cut(s) 7, 530, 622
AspLEI GCGC 2 cut(s) 347, 349
AspS9I GGNCC 2 cut(s) 507, 645
AxyI CCTNAGG 1 cut(s) 564
BaeGI GKGCMC 1 cut(s) 67
BamHI GGATCC 3 cut(s) 151, 214, 607
BbvCI CCTCAGC 1 cut(s) 177
BbvI GCAGC 2 cut(s) 272, 460
BccI CCATC 2 cut(s) 82, 619
BcgI CGANNNNNNTGC 2 cut(s) 187, 221
BciVI GTATCC 1 cut(s) 448
BclI TGATCA 2 cut(s) 168, 517
BfaI CTAG 1 cut(s) 90
BfuI GTATCC 1 cut(s) 448
BglII AGATCT 1 cut(s) 362
BisI GCNGC 2 cut(s) 261, 449
BlsI GCNGC 2 cut(s) 262, 450
BmgT120I GGNCC 2 cut(s) 507, 645
BmiI GGNNCC 3 cut(s) 153, 216, 609
BmrI ACTGGG 2 cut(s) 61, 427
BmuI ACTGGG 2 cut(s) 61, 427
Bpu10I CCTNAGC 1 cut(s) 177
BsaBI GATNNNNATC 1 cut(s) 213
Bse1I ACTGG 2 cut(s) 67, 422
Bse21I CCTNAGG 1 cut(s) 564
Bse8I GATNNNNATC 1 cut(s) 213
BseJI GATNNNNATC 1 cut(s) 213
BseMII CTCAG 2 cut(s) 168, 357
BseNI ACTGG 2 cut(s) 67, 422
BsePI GCGCGC 1 cut(s) 345
BseRI GAGGAG 1 cut(s) 554
BseSI GKGCMC 1 cut(s) 67
BseXI GCAGC 2 cut(s) 272, 460
BsgI GTGCAG 1 cut(s) 181
Bsh1236I CGCG 1 cut(s) 347
BshFI GGCC 2 cut(s) 508, 646
BsnI GGCC 2 cut(s) 508, 646
Bsp1286I GDGCHC 1 cut(s) 67
Bsp143I GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
BspANI GGCC 2 cut(s) 508, 646
BspCNI CTCAG 2 cut(s) 169, 358
BspFNI CGCG 1 cut(s) 347
BspLI GGNNCC 3 cut(s) 153, 216, 609
BspPI GGATC 6 cut(s) 146, 159, 209, 222, 602, 615
BsrI ACTGG 2 cut(s) 67, 422
BssHII GCGCGC 1 cut(s) 345
BssMI GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
Bst4CI ACNGT 1 cut(s) 354
Bst6I CTCTTC 1 cut(s) 156
BstC8I GCNNGC 3 cut(s) 45, 347, 555
BstDEI CTNAG 5 cut(s) 51, 177, 366, 564, 705
BstFNI CGCG 1 cut(s) 347
BstHHI GCGC 2 cut(s) 347, 349
BstKTI GATC 7 cut(s) 154, 171, 205, 217, 365, 520, 610
BstMBI GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
BstSLI GKGCMC 1 cut(s) 67
BstUI CGCG 1 cut(s) 347
BstV1I GCAGC 2 cut(s) 272, 460
BstX2I RGATCY 4 cut(s) 151, 214, 362, 607
BstYI RGATCY 4 cut(s) 151, 214, 362, 607
Bsu36I CCTNAGG 1 cut(s) 564
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 2 cut(s) 508, 646
BtgZI GCGATG 1 cut(s) 597
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 3 cut(s) 45, 347, 555
CfoI GCGC 2 cut(s) 347, 349
Cfr13I GGNCC 2 cut(s) 507, 645
CviAII CATG 7 cut(s) 158, 172, 232, 246, 253, 268, 573
DdeI CTNAG 5 cut(s) 51, 177, 366, 564, 705
DpnI GATC 7 cut(s) 153, 170, 204, 216, 364, 519, 609
DpnII GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
DraIII CACNNNGTG 1 cut(s) 270
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Eco57I CTGAAG 1 cut(s) 392
Eco81I CCTNAGG 1 cut(s) 564
EcoRI GAATTC 1 cut(s) 530
FaeI CATG 7 cut(s) 161, 175, 235, 249, 256, 271, 576
FatI CATG 7 cut(s) 157, 171, 231, 245, 252, 267, 572
FbaI TGATCA 2 cut(s) 168, 517
FblI GTMKAC 1 cut(s) 657
Fnu4HI GCNGC 2 cut(s) 261, 449
Fsp4HI GCNGC 2 cut(s) 261, 449
FspBI CTAG 1 cut(s) 90
GlaI GCGC 2 cut(s) 346, 348
GluI GCNGC 2 cut(s) 261, 449
HaeIII GGCC 2 cut(s) 508, 646
HhaI GCGC 2 cut(s) 347, 349
Hin1II CATG 7 cut(s) 161, 175, 235, 249, 256, 271, 576
Hin6I GCGC 2 cut(s) 345, 347
HinP1I GCGC 2 cut(s) 345, 347
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HinfI GANTC 3 cut(s) 323, 496, 577
Hpy166II GTNNAC 1 cut(s) 658
Hpy188I TCNGA 4 cut(s) 79, 341, 367, 501
Hpy188III TCNNGA 1 cut(s) 206
Hpy8I GTNNAC 1 cut(s) 658
Hpy99I CGWCG 1 cut(s) 662
HpyAV CCTTC 3 cut(s) 250, 367, 673
HpyCH4III ACNGT 1 cut(s) 354
HpyCH4V TGCA 5 cut(s) 100, 198, 448, 461, 697
HpyF3I CTNAG 5 cut(s) 51, 177, 366, 564, 705
Hsp92II CATG 7 cut(s) 161, 175, 235, 249, 256, 271, 576
HspAI GCGC 2 cut(s) 345, 347
Ksp22I TGATCA 2 cut(s) 168, 517
Kzo9I GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
LpnPI CCDG 8 cut(s) 53, 57, 80, 135, 403, 437, 539, 661
Lsp1109I GCAGC 2 cut(s) 272, 460
MaeI CTAG 1 cut(s) 90
MaeIII GTNAC 1 cut(s) 69
MalI GATC 7 cut(s) 153, 170, 204, 216, 364, 519, 609
MboI GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
MboII GAAGA 5 cut(s) 31, 153, 173, 286, 644
MfeI CAATTG 2 cut(s) 462, 476
MflI RGATCY 4 cut(s) 151, 214, 362, 607
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 6 cut(s) 7, 403, 462, 476, 530, 622
MlyI GAGTC 1 cut(s) 490
MnlI CCTC 6 cut(s) 121, 172, 203, 388, 532, 537
MseI TTAA 2 cut(s) 306, 390
MslI CAYNNNNRTG 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 451
MunI CAATTG 2 cut(s) 462, 476
MvnI CGCG 1 cut(s) 347
NdeII GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
NlaIII CATG 7 cut(s) 161, 175, 235, 249, 256, 271, 576
NlaIV GGNNCC 3 cut(s) 153, 216, 609
PauI GCGCGC 1 cut(s) 345
PfeI GAWTC 2 cut(s) 323, 577
PflFI GACNNNGTC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 262, 450
PleI GAGTC 1 cut(s) 490
PpsI GAGTC 1 cut(s) 490
PspN4I GGNNCC 3 cut(s) 153, 216, 609
PspPI GGNCC 2 cut(s) 507, 645
PsuI RGATCY 4 cut(s) 151, 214, 362, 607
PsyI GACNNNGTC 1 cut(s) 227
PteI GCGCGC 1 cut(s) 345
PvuII CAGCTG 1 cut(s) 451
RseI CAYNNNNRTG 1 cut(s) 230
SalI GTCGAC 1 cut(s) 656
SaqAI TTAA 2 cut(s) 306, 390
SatI GCNGC 2 cut(s) 261, 449
Sau3AI GATC 7 cut(s) 151, 168, 202, 214, 362, 517, 607
Sau96I GGNCC 2 cut(s) 507, 645
SchI GAGTC 1 cut(s) 490
SduI GDGCHC 1 cut(s) 67
SetI ASST 8 cut(s) 112, 124, 372, 399, 453, 555, 565, 643
SmiMI CAYNNNNRTG 1 cut(s) 230
Sse9I AATT 6 cut(s) 7, 403, 462, 476, 530, 622
SspMI CTAG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 354
TaqI TCGA 3 cut(s) 207, 620, 657
TasI AATT 6 cut(s) 7, 403, 462, 476, 530, 622
TfiI GAWTC 2 cut(s) 323, 577
Tru1I TTAA 2 cut(s) 306, 390
Tru9I TTAA 2 cut(s) 306, 390
TscAI CASTG 1 cut(s) 205
TseI GCWGC 2 cut(s) 260, 448
TspDTI ATGAA 3 cut(s) 174, 269, 645
TspRI CASTG 1 cut(s) 205
Tth111I GACNNNGTC 1 cut(s) 227
XapI RAATTY 3 cut(s) 7, 530, 622
XmiI GTMKAC 1 cut(s) 657
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.