Rorug02G0187200

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0015524
Physical Location & Seq
Reverse (-)
17453672 .. 17457280
3609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0187200.1

Sequence Viewer

Length: 870 bp
ATGGAGTTTTCAAATGAGCAAGGTGGACTATCAGAGGATGTGATTAGGAAAGCGTTTGATGCAACTGAGGAGGAGTTTGTGGACTTGGTCAAGCGGTCATGGCCGGCACGGCCAAGTATTGCTTCTGTAGGTTCATGCTGTCTTGTTGGCGCCATTACGAATGATGTCTTGTATGTGGCTAATCTCGGAGATTCGAGGGCGGTTCTTGGTCGGAGAGCAATAGATGGACCGACGGTGGTGGCAGAGCGGTTGTCTACAGATCACAATGTTGGAGTGGAGGAGGTGAGGAGGGAGGTTAAGGATCTTCACCCTGACGATTCGCACATTGTGGTTTATACTCGTGGGGTTTGGCGAATTAAGGGCATTATTCAGGTCTCTAGATCAATCGGAGATGTCTACTTGAAGAAACCTGAGTTTAACAGAGATCCTCTCTTCCAGTTTGGTATACCTGTTCCTTTAAAGAGGCCTGTAATGACATCAGTACCCTCAATATTGGTTCGGAAGTTGCAACCCCAGGACATGTTTTTGATATTTGCAACAGATGGTCTCTGGGAGCATTTGACTGATGAAGCAGCTGTTAAAATCGTCTCAAAAAATTCAAGAGTTGGAATAGCAAAGCGACTGGTAAGAACTGCCATTGAGGAGGCTGCAAGGAAAAGAGAATTGAGATATGATGACATAAAGAAACTTGAAAAGGGGGTAAGGCGCCATTTCCATGATGATATCACTGTAATTGTGATATTTCTGGATCATCCACAAACTTCCCCAAATGCTACGGATAACAGCCTCTTTACCTGCACTAGTGTTCCTGTTGATATCTTTTCTAATAATGATGATGAAGCAGTTGGCTCACTTCATACATTTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

289

Amino Acids

32.38

Weight (kDa)

6.09

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 7 - 218 2.4e-33 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 803
AccB1I GGYRCC 2 cut(s) 149, 705
AccBSI CCGCTC 1 cut(s) 247
AccI GTMKAC 3 cut(s) 254, 396, 445
AciI CCGC 3 cut(s) 94, 200, 247
AclWI GGATC 3 cut(s) 309, 419, 756
AcoI YGGCCR 2 cut(s) 101, 110
AcsI RAATTY 1 cut(s) 595
AcyI GRCGYC 2 cut(s) 150, 706
AdeI CACNNNGTG 1 cut(s) 328
AfaI GTAC 1 cut(s) 483
AflIII ACRYGT 1 cut(s) 519
AgsI TTSAA 4 cut(s) 12, 403, 600, 692
AhlI ACTAGT 1 cut(s) 800
AjnI CCWGG 1 cut(s) 513
AjuI GAANNNNNNNTTGG 2 cut(s) 106, 138
AluBI AGCT 1 cut(s) 575
AluI AGCT 1 cut(s) 575
Alw26I GTCTC 3 cut(s) 379, 551, 592
AlwI GGATC 3 cut(s) 309, 419, 756
AoxI GGCC 3 cut(s) 101, 110, 464
ApeKI GCWGC 2 cut(s) 572, 647
ApoI RAATTY 1 cut(s) 595
AspLEI GCGC 2 cut(s) 152, 708
AspS9I GGNCC 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 295, 299
AvaII GGWCC 1 cut(s) 227
BaeI ACNNNNGTAYC 2 cut(s) 465, 498
BanI GGYRCC 2 cut(s) 149, 705
BauI CACGAG 1 cut(s) 339
BbvI GCAGC 2 cut(s) 584, 634
BccI CCATC 2 cut(s) 218, 536
BceAI ACGGC 1 cut(s) 125
BciT130I CCWGG 1 cut(s) 515
BcoDI GTCTC 3 cut(s) 379, 551, 592
BcuI ACTAGT 1 cut(s) 800
BfaI CTAG 2 cut(s) 378, 801
BfmI CTRYAG 2 cut(s) 126, 255
BfoI RGCGCY 2 cut(s) 153, 709
BfuAI ACCTGC 1 cut(s) 803
BglI GCCNNNNNGGC 1 cut(s) 109
BisI GCNGC 2 cut(s) 573, 648
BlsI GCNGC 2 cut(s) 574, 649
Bme1390I CCNGG 1 cut(s) 515
Bme18I GGWCC 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 2 cut(s) 151, 707
BmrFI CCNGG 1 cut(s) 515
BmsI GCATC 1 cut(s) 49
BplI GAGNNNNNCTC 2 cut(s) 414, 446
BsaHI GRCGYC 2 cut(s) 150, 706
BsaI GGTCTC 2 cut(s) 379, 551
BsaJI CCNNGG 1 cut(s) 513
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 2 cut(s) 436, 627
BseBI CCWGG 1 cut(s) 515
BseDI CCNNGG 1 cut(s) 513
BseGI GGATG 2 cut(s) 43, 751
BseMII CTCAG 2 cut(s) 57, 402
BseNI ACTGG 2 cut(s) 436, 627
BseRI GAGGAG 5 cut(s) 83, 86, 293, 301, 656
BseXI GCAGC 2 cut(s) 584, 634
BsgI GTGCAG 1 cut(s) 781
BshFI GGCC 3 cut(s) 103, 112, 466
BshNI GGYRCC 2 cut(s) 149, 705
BsiSI CCGG 1 cut(s) 104
BsmAI GTCTC 3 cut(s) 379, 551, 592
BsmBI CGTCTC 1 cut(s) 592
BsnI GGCC 3 cut(s) 103, 112, 466
Bso31I GGTCTC 2 cut(s) 379, 551
Bsp143I GATC 5 cut(s) 259, 301, 380, 424, 748
BspACI CCGC 3 cut(s) 94, 200, 247
BspANI GGCC 3 cut(s) 103, 112, 466
BspCNI CTCAG 2 cut(s) 58, 403
BspLI GGNNCC 2 cut(s) 151, 707
BspMI ACCTGC 1 cut(s) 803
BspPI GGATC 3 cut(s) 309, 419, 756
BspT107I GGYRCC 2 cut(s) 149, 705
BspTNI GGTCTC 2 cut(s) 379, 551
BsrBI CCGCTC 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 103
BsrI ACTGG 2 cut(s) 436, 627
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 1 cut(s) 513
BssMI GATC 5 cut(s) 259, 301, 380, 424, 748
BssNAI GTATAC 1 cut(s) 446
BssNI GRCGYC 2 cut(s) 150, 706
BssSI CACGAG 1 cut(s) 339
Bst1107I GTATAC 1 cut(s) 446
Bst2BI CACGAG 1 cut(s) 339
Bst2UI CCWGG 1 cut(s) 515
Bst4CI ACNGT 2 cut(s) 235, 730
Bst6I CTCTTC 1 cut(s) 437
BstACI GRCGYC 2 cut(s) 150, 706
BstC8I GCNNGC 1 cut(s) 105
BstDEI CTNAG 2 cut(s) 66, 411
BstF5I GGATG 2 cut(s) 43, 751
BstH2I RGCGCY 2 cut(s) 153, 709
BstHHI GCGC 2 cut(s) 152, 708
BstKTI GATC 5 cut(s) 262, 304, 383, 427, 751
BstMAI GTCTC 3 cut(s) 379, 551, 592
BstMBI GATC 5 cut(s) 259, 301, 380, 424, 748
BstMWI GCNNNNNNNGC 3 cut(s) 59, 100, 109
BstNI CCWGG 1 cut(s) 515
BstNSI RCATGY 1 cut(s) 523
BstSCI CCNGG 1 cut(s) 513
BstSFI CTRYAG 2 cut(s) 126, 255
BstV1I GCAGC 2 cut(s) 584, 634
BstX2I RGATCY 2 cut(s) 301, 424
BstYI RGATCY 2 cut(s) 301, 424
BstZ17I GTATAC 1 cut(s) 446
BsuRI GGCC 3 cut(s) 103, 112, 466
BtsCI GGATG 2 cut(s) 43, 751
BtsIMutI CAGTG 1 cut(s) 726
BveI ACCTGC 1 cut(s) 803
Cac8I GCNNGC 1 cut(s) 105
CfoI GCGC 2 cut(s) 152, 708
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 1 cut(s) 227
Csp6I GTAC 1 cut(s) 482
CviAII CATG 4 cut(s) 99, 135, 520, 716
CviJI RGCY 8 cut(s) 103, 112, 179, 466, 575, 647, 786, 849
CviKI_1 RGCY 8 cut(s) 103, 112, 179, 466, 575, 647, 786, 849
CviQI GTAC 1 cut(s) 482
DdeI CTNAG 2 cut(s) 66, 411
DinI GGCGCC 2 cut(s) 151, 707
DpnI GATC 5 cut(s) 261, 303, 382, 426, 750
DpnII GATC 5 cut(s) 259, 301, 380, 424, 748
DraI TTTAAA 1 cut(s) 459
DraIII CACNNNGTG 1 cut(s) 328
EaeI YGGCCR 2 cut(s) 101, 110
Eam1104I CTCTTC 1 cut(s) 437
EarI CTCTTC 1 cut(s) 437
Eco147I AGGCCT 1 cut(s) 466
Eco31I GGTCTC 2 cut(s) 379, 551
Eco32I GATATC 2 cut(s) 724, 817
Eco47I GGWCC 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 513
EcoRV GATATC 2 cut(s) 724, 817
EgeI GGCGCC 2 cut(s) 151, 707
EheI GGCGCC 2 cut(s) 151, 707
Esp3I CGTCTC 1 cut(s) 592
FaeI CATG 4 cut(s) 102, 138, 523, 719
FalI AAGNNNNNCTT 2 cut(s) 106, 138
FatI CATG 4 cut(s) 98, 134, 519, 715
FblI GTMKAC 3 cut(s) 254, 396, 445
Fnu4HI GCNGC 2 cut(s) 573, 648
FokI GGATG 2 cut(s) 50, 738
Fsp4HI GCNGC 2 cut(s) 573, 648
FspBI CTAG 2 cut(s) 378, 801
GlaI GCGC 2 cut(s) 151, 707
GluI GCNGC 2 cut(s) 573, 648
HaeII RGCGCY 2 cut(s) 153, 709
HaeIII GGCC 3 cut(s) 103, 112, 466
HapII CCGG 1 cut(s) 104
HhaI GCGC 2 cut(s) 152, 708
Hin1I GRCGYC 2 cut(s) 150, 706
Hin1II CATG 4 cut(s) 102, 138, 523, 719
Hin6I GCGC 2 cut(s) 150, 706
HinP1I GCGC 2 cut(s) 150, 706
HinfI GANTC 2 cut(s) 191, 317
HpaII CCGG 1 cut(s) 104
HphI GGTGA 2 cut(s) 295, 299
Hpy166II GTNNAC 5 cut(s) 26, 82, 255, 397, 446
Hpy188I TCNGA 5 cut(s) 34, 188, 213, 389, 501
Hpy188III TCNNGA 3 cut(s) 378, 600, 746
Hpy8I GTNNAC 5 cut(s) 26, 82, 255, 397, 446
Hpy99I CGWCG 1 cut(s) 235
HpyCH4III ACNGT 2 cut(s) 235, 730
HpyCH4V TGCA 5 cut(s) 62, 508, 536, 650, 798
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 100, 109
HpyF3I CTNAG 2 cut(s) 66, 411
Hsp92I GRCGYC 2 cut(s) 150, 706
Hsp92II CATG 4 cut(s) 102, 138, 523, 719
HspAI GCGC 2 cut(s) 150, 706
KasI GGCGCC 2 cut(s) 149, 705
KroI GCCGGC 1 cut(s) 103
KroNI GCCGGC 1 cut(s) 105
Kzo9I GATC 5 cut(s) 259, 301, 380, 424, 748
LmnI GCTCC 1 cut(s) 553
Lsp1109I GCAGC 2 cut(s) 584, 634
LweI GCATC 1 cut(s) 49
MaeI CTAG 2 cut(s) 378, 801
MalI GATC 5 cut(s) 261, 303, 382, 426, 750
MbiI CCGCTC 1 cut(s) 247
MboI GATC 5 cut(s) 259, 301, 380, 424, 748
MboII GAAGA 3 cut(s) 296, 415, 424
MflI RGATCY 2 cut(s) 301, 424
MluCI AATT 4 cut(s) 354, 595, 662, 732
Mly113I GGCGCC 2 cut(s) 150, 706
MmeI TCCRAC 3 cut(s) 191, 250, 586
MroNI GCCGGC 1 cut(s) 103
MseI TTAA 5 cut(s) 297, 357, 417, 458, 579
MslI CAYNNNNRTG 1 cut(s) 714
MspA1I CMGCKG 1 cut(s) 575
MspI CCGG 1 cut(s) 104
MspR9I CCNGG 1 cut(s) 515
MvaI CCWGG 1 cut(s) 515
MwoI GCNNNNNNNGC 3 cut(s) 59, 100, 109
NaeI GCCGGC 1 cut(s) 105
NarI GGCGCC 2 cut(s) 150, 706
NdeII GATC 5 cut(s) 259, 301, 380, 424, 748
NgoMIV GCCGGC 1 cut(s) 103
NlaIII CATG 4 cut(s) 102, 138, 523, 719
NlaIV GGNNCC 2 cut(s) 151, 707
NspI RCATGY 1 cut(s) 523
PceI AGGCCT 1 cut(s) 466
PciI ACATGT 1 cut(s) 519
PdiI GCCGGC 1 cut(s) 105
PfeI GAWTC 2 cut(s) 191, 317
PflFI GACNNNGTC 1 cut(s) 86
PkrI GCNGC 2 cut(s) 574, 649
PluTI GGCGCC 2 cut(s) 153, 709
PscI ACATGT 1 cut(s) 519
Psp6I CCWGG 1 cut(s) 513
PspGI CCWGG 1 cut(s) 513
PspN4I GGNNCC 2 cut(s) 151, 707
PspPI GGNCC 1 cut(s) 227
PsuI RGATCY 2 cut(s) 301, 424
PsyI GACNNNGTC 1 cut(s) 86
PvuII CAGCTG 1 cut(s) 575
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
RseI CAYNNNNRTG 1 cut(s) 714
SaqAI TTAA 5 cut(s) 297, 357, 417, 458, 579
SatI GCNGC 2 cut(s) 573, 648
Sau3AI GATC 5 cut(s) 259, 301, 380, 424, 748
Sau96I GGNCC 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 515
SetI ASST 9 cut(s) 25, 133, 285, 297, 375, 412, 451, 577, 797
SfaNI GCATC 1 cut(s) 49
SfcI CTRYAG 2 cut(s) 126, 255
SfiI GGCCNNNNNGGCC 1 cut(s) 109
SfoI GGCGCC 2 cut(s) 151, 707
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 714
SpeI ACTAGT 1 cut(s) 800
Sse9I AATT 4 cut(s) 354, 595, 662, 732
SseBI AGGCCT 1 cut(s) 466
SsiI CCGC 3 cut(s) 94, 200, 247
SspDI GGCGCC 2 cut(s) 149, 705
SspI AATATT 1 cut(s) 492
SspMI CTAG 2 cut(s) 378, 801
StuI AGGCCT 1 cut(s) 466
StyD4I CCNGG 1 cut(s) 513
TaaI ACNGT 2 cut(s) 235, 730
TaqI TCGA 1 cut(s) 194
TaqII GACCGA 1 cut(s) 244
TasI AATT 4 cut(s) 354, 595, 662, 732
TfiI GAWTC 2 cut(s) 191, 317
Tru1I TTAA 5 cut(s) 297, 357, 417, 458, 579
Tru9I TTAA 5 cut(s) 297, 357, 417, 458, 579
TscAI CASTG 1 cut(s) 733
TseI GCWGC 2 cut(s) 572, 647
TspDTI ATGAA 4 cut(s) 123, 582, 845, 852
TspGWI ACGGA 1 cut(s) 791
TspRI CASTG 1 cut(s) 733
Tth111I GACNNNGTC 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 227
XapI RAATTY 1 cut(s) 595
XbaI TCTAGA 1 cut(s) 377
XceI RCATGY 1 cut(s) 523
XmiI GTMKAC 3 cut(s) 254, 396, 445
XspI CTAG 2 cut(s) 378, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.