Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0014065

Physical Location & Seq

Genomic Coordinates Reverse (-)

22493566 .. 22500099

Gene Structure 6534 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0227800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5370 bp

ATGGATAATTCCAGACCTCTATCGGGGTGTATAAATTGTGAAAGAGGTAGGTGGCGTTGGTTCCTCGTGAAAAACAAAGAGAGAAAAGGAAGAGCCAAACTTGATTCTCAAACCCGGGAGTCTTTTGGAGTGACTAAGCCCCCTGCCCTCGTTGCGCGCCACGGTTTTCTTGATTTTCCGTTCGATGCCATGAGGGGATCAAACCCGTTCACCACGTTCCGGCAGCCGGAGCCGGCCGGAATTTCGCGGCCGCAGGCTGAGCAGCGACGATTTTATCCGCAACCAGGGTCCCACCCACGCGCCGCCGTTCCGCGACGGCCTTCGCCTCGAATTCAGCGGCCGAATTTCATCGTTTATCTTCTGTCCGACAAACAGGACCTCCCGCGGTCGGACATCGAGGCGGTGATCAACCAGTGCAGGGCGAAGCCGGAGAATTTCCGGGTGAGTCCGGAGAACAGCACAGTCGCGTCGATGTTCTACGCGCAGTGGTGCGACGCGCGCGAAGCGATCGTCTGGCTGTGGGAGTCGCGGCTGGACCGGGTTCACAATTTCATGCCCAAGCTCGACGCCAACGTCTCCGTGCCGTCGGATTGGGTGGAGCTGGACGACCGGCTGAGAGCCTTGTTCGCCGGCCGAATTAAGCAGTTAATCGTCGGCGACGAGGTGAAGAAGTGTGAAGCAAAGCGCCAAAATTTGGCCAGAGAGTACGACCGAGTTCGTAAGCTGGCCAAGAGGCCTCAGAGGAATTGGGAGGACTTACCGGAGAAGGAGAAGAGGTGCAGAGTTGAGCTTGAATTGGTTGAGAGTAGGATCAGAGAGTTCAGGTCTGCTATGAACTGTTTACTTGCTCATGTTGAAGGGAAAGAGTTGGAGGAGTATGGTGAAGAAGGCTTGAAGCTGTTTAAATTCCCCGAAATTCGGTATTGGAATCGAATTCAGAGGTTAATGATGAGGGAGTGTCGCCGACTCGATGAGGGATTGCCCATTTATGCTCATAGGCAGCAAATTCTTGAGCAGATTAATAATCAGCAGGTCATGGTGTTGATTGGAGAGACTGGTTCGGGGAAAAGTACACAATTGGTTCAGTTTCTTGCTGATTCTGGAATCGCTGCTGATAAGTCCATTGTATGCACTCAGCCTCGCAAGATTGCTGCCAATTCATTGGCAAAGAGAGTCAAAGAAGAAAGTAGTGGGTGTTACTATGGAGAAAACACAGTTACCAGTTATCAGACATCATCTGGACAGCAGTTGACTTCTAAGGTAACATATATGACAGACCACTGTTTACTGCAGTACTACATGAAAGATAGAAATATGACCATGATTTCTTGCATCATAGTTGATGAGGCTCATGAAAGGACCTTGAGTACAGATCTCCTTTTAGCATTGATCAAAGATTTACTGGGTCGAAGGTCTCAGTTACGGCTTATAATAATGTCTGCAACAGCTGATGCAGAAGTGCTTTCACATTATTTTTTCAATTGTAAAATCTTTCATGTAGTGGGGAGGAACTTTCCAGTTGATGTAAGATATGCTCCCTCTTTCACTGAAGGAACTGCCAGTAATGTTGCTTCTTATGTTTCTGATGTGATGAGGGTAGCAAGAGAGATCCACAAGAATGAGAAAGAAGGGACAATTCTTGCATTTTTGACTTCTCAGATGGAAGTCGAATGGGTCTGCGAGAAGTTTATAACACCTGGTGCAATTGCATTGCCATTGCATGGGAAACTTTCTTTTGAAGAACAATCTAATGTTTTTCAAAATGACCCTGGAAAAAGAAAAATTATATTCGCCACAAATCTGGCGGAGACATCCCTGACAATTCCTGGGGTAAAGTATGTGATCGATTCTGGCATGGTCAAAGAAAGCAAATTTGAACCTGGAAGTGGTATGAATGTTCTCAGAGTTTGCAGGATCAGCCAGAGTTCTGCTAAGCAAAGAACTGGGCGTGCTGGAAGAACGGAACCTGGAATATGTTATAGACTCTACTCGGAATATGATTTTCAGGCGATGCCTCCATGTCAGGAGCCCGAGATCCGTAGAGTTCATCTTGGTGTAGCAGTTCTGAGGATTCTGGCTTTGGGTGTCAAGAATTTAAAAGAGTTTGAATTCATTGATGCACCTTGTTCTGAAGCTATAGACATGGCAATGAGGAATCTTGTTCAGTTAGGAGCTGTTAAGCAAAACAATGATGTCTATGAACTAACTCCAGAGGGTCGCAACTTGGTTAAATTGGGGGTTGAGCCTAGGCTTGGTAAATTAATTCTTGGCTGCTATCACCATAACTTGCGTAAGGAGGGCCTTGTTCTTGCGGCTGTAATGGCAAATGCAAGTAGCATATTTTGCAGAGTTGGTAATGACGAGGAGAAACTTAGGTCAGACTGCTTCAAGGTGCAATTTTGCCATCGTGATGGTGACCTCTTTACCCTTCTATCAGTGTACAAGATATGGGAAGCTCTGCCTCGAGAGAGGAAAAACACATGGTGTTGGGAAAACAGCATAAATGCCAAAACTATGAGGAGATGCCAGGACACTGTATCGGAATTGGAGTCTTGCCTTAAACATGAGCTCAATATGATAATTTCTAGTAGCTGGCGTTGGAACGCACATGTTTCAAGTGGTTGTGATATATACTTGAAAAAGGTTATACTTTCTTCCCTGGCAGAAAATGTAGCAATGTTCTCAGGATATGATCAAGTTGGTTATGAGGTGGCATTAACTGGGCAGCATGTTCGGCTGCACCCTTCTTGTTCATTGTTGGTATTCGGCCAGAAACCTAGTTGGGTTGTTTTTGGTGAACTCCTCTCAAGTTCTAATCAGTATTTGGCCTGCGTCACTACCATTGATTTTAACTCTTTGTCTACCCTTGATCCCCCTCCTGTGTTTGATGTGTCCAAGATGGAAGGTCGAAAGTTGCAACTGAAAGTGTTGACTGGTTTTGGCAGTTGTTTGCTTAAAAGATTTTGTGGGAAGGGCAACAGTTATCTGCATCATCTTGTATCTCGCGTCAGGACAATTTGCAATGACGAACTTATCAGCATTAAAGTCGATTATTATCAGAATGAGATTATGGTGTTTGCTACCCCACATAACATGGATAGGGTTATAAACTTTGTATCCGATGCATTGGAGTGTGAAAAAAGATGGCTGCGTAATGAATGCTTGGAGAAATGCTTGTATCATGGTTCTGGTGGCTTGCCTCCTGTAGCATTGTTTGGGGCCGGTGCAGAGATCAAGCATCTGGAGCTTCATAAAAGGTGTTTGACTGTTGATGTATTTCATTCAAAACTGGATGACATGGATGATAAGGAGTTTCTTACTGAGCTTGAAGAGTCTGCCTCTGGTAGTATCTGTGGTCATCATAAGTTCCCCGGCACTGGGCAGGAAAATGTTGACAAAGGAAAGGGGGCCAGGTTGACATTTCTTACTCCTGATGCTGCTCAGAAAGCTGTTCAATTAAATGATTCTGAGTTTAATGGTTCTATACTGAAGGTTGTTCCTTCACAGGTTGGAGGTGATCATAAAGTCTATCCACTCCTTGCTGTTAGAGCTAAAATCTTATGGCCTCGCAGGCAAAGCCACGGGTTTGCAATTGTTAAATGTGACATGGATGATATTTACCCCATGCTTGCTGATTTTTCTAACCTTGTAGTTGGAGGAAGAAATATTCGTTGTCAGCCTAGCAAAAGATACACGGACTCTGTTGTGATTAGTGGACTAAACAAAGATCTTTCTGAGAAAGAAATCCTTGATGCGTTAAGGACTGCTACTAGTAGGACAATCCTGGATTTTTTTCTGGTGAGAGGGGATGCTGTAGAAAATCCGCCATGTGGTGCATGTGAAGAAGCACTGCTAAGAGAAATATCACCCTATATGCCTAAACGGTACTCCCATAGTAGCTGCAGTGTTCAGGTTTTCCAGCCTGAACCAAAGAATCATTTCATGAGAGCTTTGATAACATTTGATGGAAGATTACACTTGGAGGCAGCAAAGGCTTTAGAACAATTGGAAGGGAAAGTATTGCCCGGCTTCCTTCCATGGCAAAAGATGGAGTGTCAGCAGTTATTTCATAGCTCTCTGTCCTGCCCTGGCCCTGTTTATCGTGTAATAAAGAAGCAGCTAGATCCTTTACTTGAAAGTTTTACGCATCTAAAAGGTGTAGAATGTAATCTGGAGGAGTATCCTAATGGTTCCTGCAGAGTTAAGATATCAGCCAATGCTACAAAAACGATTGCAGATCTGAGGAGACGTGTGGAGGAGCTTGTGAGGGGGAAGACTATAGACCACCCAAGCCTCACTGCAACTGTTTTGCAGCTTCTGTTTTCCAGAGATGGCACCAGTCTAATGTACTCGTTACAGCGAGAGACAGGAACATATATCATTTTTGACAGGCACAAACTCAATGTACAGGTCTTTGGTGAAGCACATAAAGTTGACATGGTCAAGCAGAAATTGGTTGAATCCCTTTTAAACCTGCATGAAAGCAAGGTGCTTGAGATCCGCCTTCAAGGTAATGCTTTACCTCCTGAGTTGATGAAAGAGGTTGTTAGAAGGTTTGGGCCAGACCTCCGGGGGCTCAAAGAGAAGGTACCAGGGGCTGATTTTACTCTAAATGTACGCCGTCAATCCATTCTCATCCACGGAAGCAAGGAGTTGAAGCAAAAAGTTGAAGAGATTATTGATGAGACTGCACACATGGCTGGTAGTTTGACTAAGAGGATAAATAGGGAAGCTGATTGTCCAATCTGCTTGTGTGATGTTGAAGACGGTTACCGGCTTGAGGACTGTGGTCACTTGTTTTGTCGTTCATGTTTGGTGGAGCAGTGCGAGTCTGCAATCAATAATCATGATAGCTTTCCGTTATGCTGCACTCATGAGGGTTGCAGGTCTCCAATTTTGATCACGGATCTCAGATCTCTTTTATCAATTGAGAAGCTGGAAGATCTCTTTAGGGCTTCGGTGGGGGCATATGTTGCTTCAAGTTGTGGCACCTACAGGTTTTGCCCATCTCCCGACTGTGCTTCAGTCTATCAGGTAGCGGCTCCTGGAACAGAAGGTGAACCATTTGTTTGTGGTGCATGTTATGCGGAGACGTGTACCATGTGCCATTTAGAACATCATCCTTACATGTCATGCAAGCAGTACAAGAATTTCAAGGAAGATCCGGATTCTTCATTGAAGGAGTGGTGCAAAGGAAAAGAACATGTGAAGAGCTGCCCGGTTTGCAGATATACAATTGAGAAGATAGATGGGTGCAACCATATAGAATGTCGGTGCGGGAAGCATATTTGCTGGGTTTGTTTGGCCTATTATGGAAGCAGCGATGAATGTTATGGCCACTTGAGAAGTGTTCACCTGGCAATCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005829 GO:0005975 GO:0005976 GO:0005977 GO:0005978 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0008194 GO:0008466 GO:0009058 GO:0009059 GO:0009250 GO:0009832 GO:0009834 GO:0009987 GO:0010383 GO:0010410 GO:0010413 GO:0010417 GO:0012505 GO:0015020 GO:0015980 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0033692 GO:0034637 GO:0034645 GO:0035251 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045492 GO:0046527 GO:0055114 GO:0070589 GO:0070592 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0080116 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_transf_8

Protein Analysis

1789

Amino Acids

202.81

Weight (kDa)

8.27

Isoelectric Point (pI)

49.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	332 - 486	4.6e-10	DEAD/DEAH box helicase
AAA_22	PF13401	342 - 479	5.6e-06	AAA domain
Helicase_C	PF00271	534 - 654	7.8e-14	Helicase conserved C-terminal domain
OB_NTP_bind	PF07717	879 - 953	5.6e-10	Oligonucleotide/oligosaccharide-binding (OB)-fold
RBD_DEAH11	PF24475	1055 - 1169	1.6e-48	DEAH11-like, RNA-binding domain
RRM_DEAH11	PF24637	1189 - 1269	4.6e-37	DEAH11 RRM domain
KH_DEAH11_1st	PF24638	1358 - 1422	4.2e-31	DEAH11 eukaryotic type KH-domain
KH_DEAH11_2nd	PF24641	1423 - 1496	2.2e-35	DEAH11 eukaryotic type KH-domain
KH_DEAH11	PF24471	1498 - 1565	9.4e-30	DEAH11-like, KH-domain
IBR	PF01485	1657 - 1712	2.8e-07	IBR domain, a half RING-finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0014065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18660 AT3G18660 AT3G18660
fragaria_vesca	FvH4_1g24050
rosa_chinensis	RchiOBHm_Chr2g0120261
rosa_laevigata	RLG00000018545
rosa_multiflora	Rmu_sc0004523.1_g000013 Rmu_sc0005172.1_g000008
rosa_roxburghii	Rroxscaffold_2G00123590
rosa_rugosa	Rorug02G0227800 Rorug02G0227900 Rorug02G0228000
rosa_samantha	Rh2AG284500 Rh2BG295500 Rh2CG273000 Rh2DG309500
rosa_wichuraiana	Rw2G022810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1430, 1691, 3107
AasI	GACNNNNNNGTC	1 cut(s)	572
Acc36I	ACCTGC	3 cut(s)	1021, 4482, 4875
Acc65I	GGTACC	1 cut(s)	4588
AccB1I	GGYRCC	3 cut(s)	4334, 4588, 4988
AccB7I	CCANNNNNTGG	2 cut(s)	694, 1721
AccI	GTMKAC	1 cut(s)	2861
AccIII	TCCGGA	2 cut(s)	448, 5164
AcoI	YGGCCR	8 cut(s)	234, 248, 338, 631, 696, 726, 2767, 5335
AcuI	CTGAAG	4 cut(s)	1569, 2151, 3509, 5007
AcyI	GRCGYC	1 cut(s)	567
AdeI	CACNNNGTG	2 cut(s)	1700, 2484
AflIII	ACRYGT	5 cut(s)	2608, 4249, 5093, 5127, 5203
AhlI	ACTAGT	1 cut(s)	3770
AjiI	CACGTC	2 cut(s)	4250, 5094
Alw21I	GWGCWC	1 cut(s)	2571
Alw26I	GTCTC	9 cut(s)	580, 1046, 1419, 1802, 4240, 4358, 4679, 4893, 5084
AlwNI	CAGNNNCTG	2 cut(s)	4318, 4598
Ama87I	CYCGRG	3 cut(s)	114, 2030, 2463
Aor13HI	TCCGGA	2 cut(s)	448, 5164
ArsI	GACNNNNNNTTYG	2 cut(s)	495, 527
AseI	ATTAAT	2 cut(s)	1020, 2261
Asp700I	GAANNNNTTC	1 cut(s)	3938
Asp718I	GGTACC	1 cut(s)	4588
AspA2I	CCTAGG	1 cut(s)	2246
AspLEI	GCGC	7 cut(s)	157, 159, 302, 484, 499, 501, 687
AspS9I	GGNCC	9 cut(s)	288, 376, 535, 1359, 2299, 3219, 3408, 4091, 4559
AsuC2I	CCSGG	8 cut(s)	115, 116, 440, 539, 3372, 4026, 4571, 5219
AvaI	CYCGRG	3 cut(s)	114, 2030, 2463
AvaII	GGWCC	4 cut(s)	288, 376, 535, 1359
AvrII	CCTAGG	1 cut(s)	2246
BalI	TGGCCA	3 cut(s)	698, 728, 5337
BanI	GGYRCC	3 cut(s)	4334, 4588, 4988
BanII	GRGCYC	3 cut(s)	2031, 2571, 4578
BarI	GAAGNNNNNNTAC	2 cut(s)	1555, 1587
BauI	CACGAG	1 cut(s)	65
BbsI	GAAGAC	2 cut(s)	4280, 4770
Bbv12I	GWGCWC	1 cut(s)	2571
BceAI	ACGGC	5 cut(s)	290, 332, 568, 1439, 4605
BcgI	CGANNNNNNTGC	4 cut(s)	2714, 2748, 3028, 3062
BciVI	GTATCC	2 cut(s)	3127, 4191
BclI	TGATCA	5 cut(s)	405, 1389, 2692, 3517, 4899
BcnI	CCSGG	8 cut(s)	115, 116, 440, 539, 3372, 4026, 4571, 5219
BcoDI	GTCTC	9 cut(s)	580, 1046, 1419, 1802, 4240, 4358, 4679, 4893, 5084
BcuI	ACTAGT	1 cut(s)	3770
BfaI	CTAG	6 cut(s)	2247, 2586, 2778, 3681, 3771, 4121
BfmI	CTRYAG	8 cut(s)	1289, 2136, 3204, 3813, 3901, 4195, 4278, 4993
BfoI	RGCGCY	1 cut(s)	688
BfuAI	ACCTGC	3 cut(s)	1021, 4482, 4875
BfuI	GTATCC	2 cut(s)	3127, 4191
BglI	GCCNNNNNGGC	1 cut(s)	3571
BglII	AGATCT	5 cut(s)	1372, 3727, 4237, 4913, 4942
BlnI	CCTAGG	1 cut(s)	2246
BlpI	GCTNAGC	2 cut(s)	258, 1932
BmcAI	AGTACT	1 cut(s)	1295
Bme18I	GGWCC	4 cut(s)	288, 376, 535, 1359
BmeT110I	CYCGRG	3 cut(s)	114, 2030, 2463
BmgBI	CACGTC	2 cut(s)	4250, 5094
BmgT120I	GGNCC	9 cut(s)	288, 376, 535, 1359, 2299, 3219, 3408, 4091, 4559
BmrI	ACTGGG	4 cut(s)	1412, 1953, 2730, 3387
BmuI	ACTGGG	4 cut(s)	1412, 1953, 2730, 3387
BoxI	GACNNNNGTC	1 cut(s)	4788
BpiI	GAAGAC	2 cut(s)	4280, 4770
BplI	GAGNNNNNCTC	2 cut(s)	3323, 3355
BpmI	CTGGAG	3 cut(s)	2193, 3263, 4193
Bpu1102I	GCTNAGC	2 cut(s)	258, 1932
BpuEI	CTTGAG	6 cut(s)	1031, 1384, 2791, 4514, 4799, 5362
BpuMI	CCSGG	8 cut(s)	115, 116, 440, 539, 3372, 4026, 4571, 5219
Bsa29I	ATCGAT	1 cut(s)	1845
BsaBI	GATNNNNATC	1 cut(s)	3054
BsaHI	GRCGYC	1 cut(s)	567
BsaI	GGTCTC	2 cut(s)	1419, 4893
BsaWI	WCCGGW	3 cut(s)	448, 760, 5164
BsaXI	ACNNNNNCTCC	4 cut(s)	363, 393, 761, 791
Bse118I	RCCGGY	5 cut(s)	232, 609, 629, 3221, 4773
Bse3DI	GCAATG	5 cut(s)	1709, 1715, 2154, 2682, 3028
Bse8I	GATNNNNATC	1 cut(s)	3054
BseAI	TCCGGA	2 cut(s)	448, 5164
BseCI	ATCGAT	1 cut(s)	1845
BseGI	GGATG	7 cut(s)	1811, 3298, 3307, 3616, 3814, 4635, 5119
BseJI	GATNNNNATC	1 cut(s)	3054
BseMI	GCAATG	5 cut(s)	1709, 1715, 2154, 2682, 3028
BsePI	GCGCGC	2 cut(s)	155, 497
BseRI	GAGGAG	7 cut(s)	887, 2378, 2533, 2792, 4190, 4258, 4271
BseX3I	CGGCCG	4 cut(s)	234, 248, 338, 631
BseYI	CCCAGC	1 cut(s)	5292
BsgI	GTGCAG	6 cut(s)	436, 799, 2723, 3246, 4674, 4852
Bsh1285I	CGRYCG	8 cut(s)	237, 251, 341, 389, 510, 610, 634, 712
BshNI	GGYRCC	3 cut(s)	4334, 4588, 4988
BshVI	ATCGAT	1 cut(s)	1845
BsiEI	CGRYCG	8 cut(s)	237, 251, 341, 389, 510, 610, 634, 712
BsiHKAI	GWGCWC	1 cut(s)	2571
BsiHKCI	CYCGRG	3 cut(s)	114, 2030, 2463
BslFI	GGGAC	2 cut(s)	274, 1645
BsmAI	GTCTC	9 cut(s)	580, 1046, 1419, 1802, 4240, 4358, 4679, 4893, 5084
BsmBI	CGTCTC	3 cut(s)	580, 4240, 5084
BsmFI	GGGAC	2 cut(s)	274, 1645
BsmI	GAATGC	1 cut(s)	3164
Bso31I	GGTCTC	2 cut(s)	1419, 4893
BsoBI	CYCGRG	3 cut(s)	114, 2030, 2463
Bsp1286I	GDGCHC	3 cut(s)	2031, 2571, 4578
Bsp13I	TCCGGA	2 cut(s)	448, 5164
Bsp1407I	TGTACA	2 cut(s)	2439, 4405
Bsp1720I	GCTNAGC	2 cut(s)	258, 1932
Bsp19I	CCATGG	1 cut(s)	4037
BspDI	ATCGAT	1 cut(s)	1845
BspEI	TCCGGA	2 cut(s)	448, 5164
BspHI	TCATGA	4 cut(s)	1351, 3942, 4846, 4873
BspMAI	CTGCAG	3 cut(s)	1293, 3905, 4199
BspMI	ACCTGC	3 cut(s)	1021, 4482, 4875
BspQI	GCTCTTC	2 cut(s)	85, 5204
BspT107I	GGYRCC	3 cut(s)	4334, 4588, 4988
BspTNI	GGTCTC	2 cut(s)	1419, 4893
BsrDI	GCAATG	5 cut(s)	1709, 1715, 2154, 2682, 3028
BsrFI	RCCGGY	5 cut(s)	232, 609, 629, 3221, 4773
BsrGI	TGTACA	2 cut(s)	2439, 4405
BssAI	RCCGGY	5 cut(s)	232, 609, 629, 3221, 4773
BssHII	GCGCGC	2 cut(s)	155, 497
BssNI	GRCGYC	1 cut(s)	567
BssSI	CACGAG	1 cut(s)	65
BssT1I	CCWWGG	2 cut(s)	2246, 4037
Bst2BI	CACGAG	1 cut(s)	65
Bst6I	CTCTTC	5 cut(s)	85, 767, 3324, 4665, 5204
BstACI	GRCGYC	1 cut(s)	567
BstAPI	GCANNNNNTGC	3 cut(s)	2343, 4973, 5084
BstAUI	TGTACA	2 cut(s)	2439, 4405
BstDSI	CCRYGG	5 cut(s)	160, 383, 3580, 4037, 4639
BstEII	GGTNACC	2 cut(s)	2414, 4769
BstF5I	GGATG	7 cut(s)	1811, 3298, 3307, 3616, 3814, 4635, 5119
BstH2I	RGCGCY	1 cut(s)	688
BstHHI	GCGC	7 cut(s)	157, 159, 302, 484, 499, 501, 687
BstMAI	GTCTC	9 cut(s)	580, 1046, 1419, 1802, 4240, 4358, 4679, 4893, 5084
BstMCI	CGRYCG	8 cut(s)	237, 251, 341, 389, 510, 610, 634, 712
BstNSI	RCATGY	6 cut(s)	2612, 2732, 3840, 5082, 5131, 5207
BstPAI	GACNNNNGTC	1 cut(s)	4788
BstPI	GGTNACC	2 cut(s)	2414, 4769
BstSFI	CTRYAG	8 cut(s)	1289, 2136, 3204, 3813, 3901, 4195, 4278, 4993
BstV2I	GAAGAC	2 cut(s)	4280, 4770
BstXI	CCANNNNNNTGG	3 cut(s)	1162, 1801, 2411
BstZI	CGGCCG	4 cut(s)	234, 248, 338, 631
Bsu15I	ATCGAT	1 cut(s)	1845
BsuI	GTATCC	2 cut(s)	3127, 4191
BsuTUI	ATCGAT	1 cut(s)	1845
BtgI	CCRYGG	5 cut(s)	160, 383, 3580, 4037, 4639
BtgZI	GCGATG	2 cut(s)	2024, 5337
BtrI	CACGTC	2 cut(s)	4250, 5094
BtsCI	GGATG	7 cut(s)	1811, 3298, 3307, 3616, 3814, 4635, 5119
BtsI	GCAGTG	5 cut(s)	491, 3848, 3910, 4296, 4829
BveI	ACCTGC	3 cut(s)	1021, 4482, 4875
CaiI	CAGNNNCTG	2 cut(s)	4318, 4598
CciI	TCATGA	4 cut(s)	1351, 3942, 4846, 4873
CciNI	GCGGCCGC	1 cut(s)	248
CfoI	GCGC	7 cut(s)	157, 159, 302, 484, 499, 501, 687
Cfr10I	RCCGGY	5 cut(s)	232, 609, 629, 3221, 4773
Cfr13I	GGNCC	9 cut(s)	288, 376, 535, 1359, 2299, 3219, 3408, 4091, 4559
Cfr42I	CCGCGG	1 cut(s)	386
Cfr9I	CCCGGG	1 cut(s)	114
ClaI	ATCGAT	1 cut(s)	1845
CseI	GACGC	5 cut(s)	456, 503, 575, 2821, 2995
CsiI	ACCWGGT	1 cut(s)	1696
DraI	TTTAAA	3 cut(s)	904, 2097, 4470
DraIII	CACNNNGTG	2 cut(s)	1700, 2484
DrdI	GACNNNNNNGTC	1 cut(s)	572
DseDI	GACNNNNNNGTC	1 cut(s)	572
EaeI	YGGCCR	8 cut(s)	234, 248, 338, 631, 696, 726, 2767, 5335
EagI	CGGCCG	4 cut(s)	234, 248, 338, 631
Eam1104I	CTCTTC	5 cut(s)	85, 767, 3324, 4665, 5204
EarI	CTCTTC	5 cut(s)	85, 767, 3324, 4665, 5204
EciI	GGCGGA	3 cut(s)	1820, 3813, 4490
Ecl136II	GAGCTC	1 cut(s)	2569
EclXI	CGGCCG	4 cut(s)	234, 248, 338, 631
Eco130I	CCWWGG	2 cut(s)	2246, 4037
Eco147I	AGGCCT	1 cut(s)	736
Eco24I	GRGCYC	3 cut(s)	2031, 2571, 4578
Eco31I	GGTCTC	2 cut(s)	1419, 4893
Eco32I	GATATC	1 cut(s)	4209
Eco47I	GGWCC	4 cut(s)	288, 376, 535, 1359
Eco52I	CGGCCG	4 cut(s)	234, 248, 338, 631
Eco53kI	GAGCTC	1 cut(s)	2569
Eco57I	CTGAAG	4 cut(s)	1569, 2151, 3509, 5007
Eco88I	CYCGRG	3 cut(s)	114, 2030, 2463
Eco91I	GGTNACC	2 cut(s)	2414, 4769
EcoICRI	GAGCTC	1 cut(s)	2569
EcoO109I	RGGNCCY	4 cut(s)	288, 376, 1359, 2299
EcoO65I	GGTNACC	2 cut(s)	2414, 4769
EcoRI	GAATTC	3 cut(s)	330, 933, 2108
EcoRV	GATATC	1 cut(s)	4209
EcoT14I	CCWWGG	2 cut(s)	2246, 4037
EcoT22I	ATGCAT	1 cut(s)	3127
EcoT38I	GRGCYC	3 cut(s)	2031, 2571, 4578
ErhI	CCWWGG	2 cut(s)	2246, 4037
Esp3I	CGTCTC	3 cut(s)	580, 4240, 5084
FalI	AAGNNNNNCTT	2 cut(s)	3732, 3764
FaqI	GGGAC	2 cut(s)	274, 1645
FauI	CCCGC	2 cut(s)	390, 5270
FauNDI	CATATG	1 cut(s)	4969
FbaI	TGATCA	5 cut(s)	405, 1389, 2692, 3517, 4899
FblI	GTMKAC	1 cut(s)	2861
FokI	GGATG	7 cut(s)	1798, 3305, 3314, 3623, 3821, 4622, 5106
FriOI	GRGCYC	3 cut(s)	2031, 2571, 4578
FspBI	CTAG	6 cut(s)	2247, 2586, 2778, 3681, 3771, 4121
GlaI	GCGC	7 cut(s)	156, 158, 301, 483, 498, 500, 686
GsaI	CCCAGC	1 cut(s)	5296
GsuI	CTGGAG	3 cut(s)	2193, 3263, 4193
HaeII	RGCGCY	1 cut(s)	688
HgaI	GACGC	5 cut(s)	456, 503, 575, 2821, 2995
HhaI	GCGC	7 cut(s)	157, 159, 302, 484, 499, 501, 687
Hin1I	GRCGYC	1 cut(s)	567
Hin6I	GCGC	7 cut(s)	155, 157, 300, 482, 497, 499, 685
HinP1I	GCGC	7 cut(s)	155, 157, 300, 482, 497, 499, 685
HincII	GTYRAC	5 cut(s)	1251, 2931, 3394, 3417, 4435
HindII	GTYRAC	5 cut(s)	1251, 2931, 3394, 3417, 4435
Hpy99I	CGWCG	8 cut(s)	270, 318, 472, 497, 569, 589, 656, 662
HpyCH4IV	ACGT	4 cut(s)	215, 573, 4249, 5093
HpySE526I	ACGT	4 cut(s)	215, 573, 4249, 5093
Hsp92I	GRCGYC	1 cut(s)	567
HspAI	GCGC	7 cut(s)	155, 157, 300, 482, 497, 499, 685
KflI	GGGWCCC	1 cut(s)	288
Kpn2I	TCCGGA	2 cut(s)	448, 5164
KpnI	GGTACC	1 cut(s)	4592
KroI	GCCGGC	2 cut(s)	232, 629
KroNI	GCCGGC	2 cut(s)	234, 631
Ksp22I	TGATCA	5 cut(s)	405, 1389, 2692, 3517, 4899
KspI	CCGCGG	1 cut(s)	386
LguI	GCTCTTC	2 cut(s)	85, 5204
LmnI	GCTCC	9 cut(s)	229, 598, 1540, 2026, 2171, 3244, 4258, 4819, 5047
MabI	ACCWGGT	1 cut(s)	1696
MaeI	CTAG	6 cut(s)	2247, 2586, 2778, 3681, 3771, 4121
MaeII	ACGT	4 cut(s)	215, 573, 4249, 5093
MauBI	CGCGCGCG	1 cut(s)	497
MfeI	CAATTG	7 cut(s)	1076, 1480, 1704, 3591, 4004, 4926, 5235
MhlI	GDGCHC	3 cut(s)	2031, 2571, 4578
MlsI	TGGCCA	3 cut(s)	698, 728, 5337
MluNI	TGGCCA	3 cut(s)	698, 728, 5337
MmeI	TCCRAC	7 cut(s)	369, 390, 567, 849, 2579, 3490, 3634
Mox20I	TGGCCA	3 cut(s)	698, 728, 5337
Mph1103I	ATGCAT	1 cut(s)	3127
MroI	TCCGGA	2 cut(s)	448, 5164
MroNI	GCCGGC	2 cut(s)	232, 629
MroXI	GAANNNNTTC	1 cut(s)	3938
MscI	TGGCCA	3 cut(s)	698, 728, 5337
MslI	CAYNNNNRTG	5 cut(s)	1617, 2052, 2147, 2409, 3130
Msp20I	TGGCCA	3 cut(s)	698, 728, 5337
MspA1I	CMGCKG	3 cut(s)	337, 385, 1448
MunI	CAATTG	7 cut(s)	1076, 1480, 1704, 3591, 4004, 4926, 5235
Mva1269I	GAATGC	1 cut(s)	3164
NaeI	GCCGGC	2 cut(s)	234, 631
NciI	CCSGG	8 cut(s)	115, 116, 440, 539, 3372, 4026, 4571, 5219
NcoI	CCATGG	1 cut(s)	4037
NdeI	CATATG	1 cut(s)	4969
NgoMIV	GCCGGC	2 cut(s)	232, 629
NmuCI	GTSAC	5 cut(s)	130, 2414, 2833, 3602, 4790
NotI	GCGGCCGC	1 cut(s)	248
NsiI	ATGCAT	1 cut(s)	3127
NspI	RCATGY	6 cut(s)	2612, 2732, 3840, 5082, 5131, 5207
PaeR7I	CTCGAG	1 cut(s)	2463
PagI	TCATGA	4 cut(s)	1351, 3942, 4846, 4873
PauI	GCGCGC	2 cut(s)	155, 497
PceI	AGGCCT	1 cut(s)	736
PciI	ACATGT	3 cut(s)	2608, 5127, 5203
PciSI	GCTCTTC	2 cut(s)	85, 5204
PcsI	WCGNNNNNNNCGW	2 cut(s)	570, 657
PctI	GAATGC	1 cut(s)	3164
PdiI	GCCGGC	2 cut(s)	234, 631
PdmI	GAANNNNTTC	1 cut(s)	3938
PflFI	GACNNNGTC	1 cut(s)	4439
PflMI	CCANNNNNTGG	2 cut(s)	694, 1721
PfoI	TCCNGGA	2 cut(s)	3783, 5044
Ple19I	CGATCG	1 cut(s)	510
PpuMI	RGGWCCY	3 cut(s)	288, 376, 1359
PscI	ACATGT	3 cut(s)	2608, 5127, 5203
PshAI	GACNNNNGTC	1 cut(s)	4788
PshBI	ATTAAT	2 cut(s)	1020, 2261
PsiI	TTATAA	3 cut(s)	1430, 1691, 3107
Psp124BI	GAGCTC	1 cut(s)	2571
Psp5II	RGGWCCY	3 cut(s)	288, 376, 1359
PspEI	GGTNACC	2 cut(s)	2414, 4769
PspFI	CCCAGC	1 cut(s)	5292
PspPI	GGNCC	9 cut(s)	288, 376, 535, 1359, 2299, 3219, 3408, 4091, 4559
PspPPI	RGGWCCY	3 cut(s)	288, 376, 1359
PstI	CTGCAG	3 cut(s)	1293, 3905, 4199
PstNI	CAGNNNCTG	2 cut(s)	4318, 4598
PsyI	GACNNNGTC	1 cut(s)	4439
PteI	GCGCGC	2 cut(s)	155, 497
PvuI	CGATCG	1 cut(s)	510
PvuII	CAGCTG	1 cut(s)	1448
RseI	CAYNNNNRTG	5 cut(s)	1617, 2052, 2147, 2409, 3130
SacI	GAGCTC	1 cut(s)	2571
SacII	CCGCGG	1 cut(s)	386
SapI	GCTCTTC	2 cut(s)	85, 5204
Sau96I	GGNCC	9 cut(s)	288, 376, 535, 1359, 2299, 3219, 3408, 4091, 4559
ScaI	AGTACT	1 cut(s)	1295
SduI	GDGCHC	3 cut(s)	2031, 2571, 4578
SexAI	ACCWGGT	1 cut(s)	1696
SfcI	CTRYAG	8 cut(s)	1289, 2136, 3204, 3813, 3901, 4195, 4278, 4993
Sfr274I	CTCGAG	1 cut(s)	2463
Sfr303I	CCGCGG	1 cut(s)	386
SgrBI	CCGCGG	1 cut(s)	386
SinI	GGWCC	4 cut(s)	288, 376, 535, 1359
SlaI	CTCGAG	1 cut(s)	2463
SmaI	CCCGGG	1 cut(s)	116
SmiMI	CAYNNNNRTG	5 cut(s)	1617, 2052, 2147, 2409, 3130
SmlI	CTYRAG	7 cut(s)	1010, 1363, 2463, 2806, 4493, 4778, 5341
SmoI	CTYRAG	7 cut(s)	1010, 1363, 2463, 2806, 4493, 4778, 5341
SpeI	ACTAGT	1 cut(s)	3770
SseBI	AGGCCT	1 cut(s)	736
SspI	AATATT	1 cut(s)	3667
SspMI	CTAG	6 cut(s)	2247, 2586, 2778, 3681, 3771, 4121
SstI	GAGCTC	1 cut(s)	2571
StuI	AGGCCT	1 cut(s)	736
StyI	CCWWGG	2 cut(s)	2246, 4037
TaiI	ACGT	4 cut(s)	218, 576, 4252, 5096
TaqII	GACCGA	1 cut(s)	726
TatI	WGTACW	7 cut(s)	1070, 1293, 1367, 2439, 4347, 4405, 5142
TauI	GCSGC	7 cut(s)	250, 253, 305, 340, 532, 2315, 5042
TseFI	GTSAC	5 cut(s)	130, 2414, 2833, 3602, 4790
Tsp45I	GTSAC	5 cut(s)	130, 2414, 2833, 3602, 4790
TspGWI	ACGGA	8 cut(s)	168, 568, 1976, 2027, 3710, 4656, 4848, 4919
TspMI	CCCGGG	1 cut(s)	114
Tth111I	GACNNNGTC	1 cut(s)	4439
Van91I	CCANNNNNTGG	2 cut(s)	694, 1721
VpaK11BI	GGWCC	4 cut(s)	288, 376, 535, 1359
VspI	ATTAAT	2 cut(s)	1020, 2261
XceI	RCATGY	6 cut(s)	2612, 2732, 3840, 5082, 5131, 5207
XhoI	CTCGAG	1 cut(s)	2463
XmaI	CCCGGG	1 cut(s)	114
XmaJI	CCTAGG	1 cut(s)	2246
XmiI	GTMKAC	1 cut(s)	2861
XmnI	GAANNNNTTC	1 cut(s)	3938
XspI	CTAG	6 cut(s)	2247, 2586, 2778, 3681, 3771, 4121
ZrmI	AGTACT	1 cut(s)	1295
Zsp2I	ATGCAT	1 cut(s)	3127

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.