Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0014065

Physical Location & Seq

Genomic Coordinates Reverse (-)

22523192 .. 22534961

Gene Structure 11770 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0228000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3705 bp

ATGGGTTTACCAAAAGCCAAGAATGCCTCTGCTTCGGAGGGGACTGATGACAATATTACTGCCTCAATCGGGCTAAAAAGGAAACGTGGAAGAACAAGTATGGAGCGCATAGTGAACCGAGCACTTAGAGGTAAGAAATCAGTAGTTGAGTTTAACCCAAAGGGCGTACCATTTGGGAAGGCAGCAGCAGAGATGGCTTCTTACATAGGGGTGATTGTGCGTACCACGGTTCCCATCATTGTTGAGAGCTGGCCTAAGGTGGAGAAGGATCTGAAAAATGAAATTTGGAAATCCGTAGAGATGGCATTCGTCTTAGCTCCTAGATGCAGAAAAATGGTCTTGTCATCTGCGGCCAATAAGTGGAGGCAATTCAAGTCAGAATTGACAACAAAATACGTACTGCCATATAAAGATCAGCCTGATGCTTTAAAAGACCCACCAGAAGAATATGATTTCATTAAGCAACAAGATTGGGAACAATTCGTAAAGAGTCGCTTAACCACAGATTTTCAGAAACTCCACATGGAGCAGAAAGAGAGAAGGGGAAAGTTGCAGAATGCTCATAGAATGTCACGAAAGGGATATGCTGGTTTAGAGGCAGAACTAAAAAAAACTATGAATGAAGATGAACTTGATCTAGCTGTCCTCTGGAAGAAAGGACGTGAGGACAAAAATGGGAATATCTCCCATGAGACTGTAGGGGAGCAGGCTGCAGAAATGGATACTTTAATGAACAATGAAGGGGATATTTCCAACTCTAACAGTGGATCAAGTGATGATGTCAGGTCAATGGGGTTGGGGACTCCAGAACATTCTAGTAGGGTTAGAGGTGCAGGAGAGTCTCTCATGCCAAATGTTTCACCTCCTCAGTTGGAGCGGGAAAGTGTCATGGATGAGGTCAGAAAGATGATAGAGCAGCAAAGGTTATGGTTTGAAGCCAAGATTTCTTTGTTAGAGGCAAAGATCAGTGGTGATTGCCCAGCCACAAGTATCACACTTCCAACTCCTCTGCTTGCCAAGCCTTCAAAGAAGGGCAGGTGTTCTGGGAAGACAAATGTTGAGGATAATGAAATTGATTCGGAAGCTTTCTCATTTGTGGGCAGAAGAGAATTCGTGAAGGGAAAATCTTGCAAGTTGGCTGTTGGATCAATCAACAATGTTGTTTCCCATGGTACAATAATTGAAATGGATGTGGCGAACCACACAGTCCATGGTGTGCCTTTGGGAGAGGGCAATATACGTGTTGCTATTGATACTGCCCTAGATGAACAAGCGCTGCTTCCCATACCAGTTACAGGGGAATTAGCAACTGTTGGGCAAGCTGTAGGCTCTCATGTGGCTTGGCCGAAACATCTTGTTAAGTTGATGAATGAAGAGGAACGTGGAAATTCTTCTATAAAACCTCGAGATTTACCCAATCAAGATGTGATCCTGCCCAAGTCCCTCAAGCTCCTATACCGTTATGCTGAGCGTGCCATGACGGATGGTGAGCCTATCAGTGTCTTTATGGAAGAAGCCGTATTTGGTATTGCCAAGACTCTCAACATTTTCAAAGAAGATGTTATGCAATTTATGGAAATGAAGGAAATCCCCCCTAGGTGCATAACAGTGTACATGAGGCACCTCTATGATATGCTAAAGCAGTCCAACATGGCCAATATGGTTGGTTTGATGGACCCTTCAAGCATATCAGTTGGGGAAGGCAATTCTGACCACAAATCACAGGTGCTCGCAACCCGGTTGCAGCAAGGGTCTGCTGACCAAATACTTTTGGTACCTTACAATTCTGGTTATCATTGGATGCTGACCATCATCAGTGAAGACAAAGAAGTATGCTACTTCATGGACCCTCTTCAACGCTATTTCATGGACCCTCTCCGAAGAAGCATGCGTGAGGAAGAATGGAAATATGTTGTAAACAATGGGATTAGACAATTCAACATTGAAACGGGTAGGGGATTCCGAAAACAACCTCTATGGAAAGTATTGATGGGTCCTAAACAACCCTCGAATATGGAGTGTGGGTATTATGTGATGAGATATATGAAAGAAATCATAGAAGGTCATGACCTTTCCTTTGCAACAAAGTGGGATGGGAGGAAACTAAATGCCTACACACAGACAGAACTGGATGAGGTGAGAAGGAACAATTGTTTATATCCCGGGACTTCGGTTAAGCCAAATGGGTGCGTATCTGATTTTGTTGCACCGAGTTTGACTCGGAAGTTGTGCTTTGGTTTGAGGGGTGATTTCCAGGCAAGGGCTATGGTGATATCTGGAGAAGATGATCTCCTGTCTCACACTAATGGGGTTGAAGCACAACCTGACGCATTGAGTTTTGGAACACTTGCAGCTGATATGGCTCCTCTTAGTAGCGGTTTCTCTTCTGATAACGATGAATACGATCTAGATTATCCTATTGAAGGATTTTCTTCCATTGCGGAGGCCATTGAAGATATTCGTCAAGGCAAGATGGTAATTGTTGTAGATGATGAAGATAGAGAAAACGAGGGAGATCTTATAATGGCAGCATCCTTGGCAACACCAGAAGCTATGGCTTTTATTGTCAAGCACGGAACCGGAATTGTTTGTGTGAGCATGAAAGGAGAAGACTTAGAAAGGTTACAACTTCCGTTGATGGTGACACAGAATGAAGAAAAACTTTGTACAGCATTCACTGTCTCAGTGGATGCAAAACATGGTACGACAACTGGTGTTTCAGCTCGTGATAGAGCTGCAACAGTCTTGGCCCTTGCATCAAGGGATTCAAAACCTGAAGACTTCAACCGACCAGGTCATATCTTTCCCCTAAAGTACAGGGAGGGAGGAGTTCTAAAAAGAGCTGGGCACACAGAAGCTTCAGTTGATCTCGCAATGTTGGCTGGGCTAGATCCGGTAGCAGTTTTGTGTGAAATTGTGGATGATGACGGTTCTATGGCTAGATTACCGAGGCTTCGTGAATTTGCAAAGGCAGAAAACTTGAAAATTATTTCTATTGCTGATTTGATAAGATATAGGAGAAAAAGGGACAAGTTGGTGGAGCGGGCTGGAGCTGCACGGATACCCACAATGTGGGGGCCATTTGAAGCCTACTGTTATAGGTCATTGCTAGATGGGATTGAGCACATTGCTATGGTTAAAGGTGATATTGGAGATGGGCAAAATATACTTGTGAGAGTACATTCAGAGTGTCTCACCGGTGACATCTTTGGGTCGGCCAGATGTGATTGTGGAAACCAGCTGGCACTTGCTATGAAACAAATTGAAGCAGCGGGTAGGGGTGTTTTAGTGTATCTCCGTGGACATGAAGGTAGAGGAATTGGTTTAGGCCACAAGCTCCGAGCTTATAACCTGCAGGATGATGGGCGTGACACTGTTGAAGCCAACGAGGAGCTCGGACTACCTGTTGATTCTCGCGAGTATGGAATTGGTGCACAGATACTACGAGATCTAGGTGTTCGTACAATGAAGCTGATGACAAACAATCCTGCAAAGTATAGCGGGCTCAAAGGTTATGGTTTGGCCGTTGCTGGCAGGGTCCCGTTATTGACACCAATAACTATGGAGAACAAGAGATATTTGGAGACTAAACGAACAAAAATGGGGCATGTCTATGGCTCCAATCTCAATGGTCATGTAGATGGCACCAGTGATGAAAGCAGTTCAAGCATTGGCAACCCATCTAATGGTGTCTCTGAGACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005829 GO:0005975 GO:0005976 GO:0005977 GO:0005978 GO:0006073 GO:0006091 GO:0006112 GO:0008150 GO:0008152 GO:0008194 GO:0008466 GO:0009058 GO:0009059 GO:0009250 GO:0009832 GO:0009834 GO:0009987 GO:0010383 GO:0010410 GO:0010413 GO:0010417 GO:0012505 GO:0015020 GO:0015980 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0033692 GO:0034637 GO:0034645 GO:0035251 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045492 GO:0046527 GO:0055114 GO:0070589 GO:0070592 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0080116 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_transf_8

Protein Analysis

1234

Amino Acids

136.95

Weight (kDa)

6.15

Isoelectric Point (pI)

37.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Transposase_24	PF03004	156 - 279	1.3e-07	Plant transposase (Ptta/En/Spm family)
DUF8039	PF26133	375 - 455	1.6e-13	Domain of unknown function (DUF8039)
Peptidase_C48	PF02902	589 - 695	1.8e-06	Ulp1 protease family, C-terminal catalytic domain
DHBP_synthase	PF00926	815 - 1005	1.8e-87	3,4-dihydroxy-2-butanone 4-phosphate synthase
GTP_cyclohydro2	PF00925	1016 - 1181	6.1e-71	GTP cyclohydrolase II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0014065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18660 AT3G18660 AT3G18660
fragaria_vesca	FvH4_1g24050
rosa_chinensis	RchiOBHm_Chr2g0120261
rosa_laevigata	RLG00000018545
rosa_multiflora	Rmu_sc0004523.1_g000013 Rmu_sc0005172.1_g000008
rosa_roxburghii	Rroxscaffold_2G00123590
rosa_rugosa	Rorug02G0227800 Rorug02G0227900 Rorug02G0228000
rosa_samantha	Rh2AG284500 Rh2BG295500 Rh2CG273000 Rh2DG309500
rosa_wichuraiana	Rw2G022810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2522, 3348
AarI	CACCTGC	1 cut(s)	1026
Acc36I	ACCTGC	2 cut(s)	1026, 3360
Acc65I	GGTACC	1 cut(s)	1774
AccB1I	GGYRCC	3 cut(s)	1620, 1774, 3644
AccB7I	CCANNNNNTGG	3 cut(s)	360, 3072, 3686
AccBSI	CCGCTC	2 cut(s)	877, 3043
AccII	CGCG	1 cut(s)	3417
AciI	CCGC	7 cut(s)	350, 877, 2376, 2441, 3043, 3272, 3501
AclWI	GGATC	5 cut(s)	276, 775, 1153, 1423, 2885
AcoI	YGGCCR	5 cut(s)	351, 1343, 1653, 3216, 3522
AcsI	RAATTY	4 cut(s)	282, 1109, 1387, 2960
AcuI	CTGAAG	2 cut(s)	2796, 2844
AdeI	CACNNNGTG	1 cut(s)	3072
AfeI	AGCGCT	1 cut(s)	1275
AfiI	CCNNNNNNNGG	7 cut(s)	69, 360, 1295, 2260, 2423, 3072, 3686
AflIII	ACRYGT	1 cut(s)	1240
AgeI	ACCGGT	1 cut(s)	3197
AjiI	CACGTC	1 cut(s)	662
AjnI	CCWGG	2 cut(s)	2253, 2791
AjuI	GAANNNNNNNTTGG	2 cut(s)	1506, 1538
Alw21I	GWGCWC	5 cut(s)	124, 1731, 3126, 3396, 3436
Alw26I	GTCTC	8 cut(s)	686, 846, 2301, 2686, 3197, 3578, 3692, 3697
Alw44I	GTGCAC	1 cut(s)	3432
AlwI	GGATC	5 cut(s)	276, 775, 1153, 1423, 2885
Ama87I	CYCGRG	2 cut(s)	1404, 2162
Aor51HI	AGCGCT	1 cut(s)	1275
ApaLI	GTGCAC	1 cut(s)	3432
ApoI	RAATTY	4 cut(s)	282, 1109, 1387, 2960
ArsI	GACNNNNNNTTYG	2 cut(s)	2060, 2092
AsiGI	ACCGGT	1 cut(s)	3197
Asp700I	GAANNNNTTC	2 cut(s)	1390, 2457
Asp718I	GGTACC	1 cut(s)	1774
AspA2I	CCTAGG	1 cut(s)	1595
AspLEI	GCGC	2 cut(s)	108, 1276
AspS9I	GGNCC	7 cut(s)	1675, 1846, 1870, 1994, 2749, 3077, 3538
AsuC2I	CCSGG	3 cut(s)	1738, 2163, 2164
AvaI	CYCGRG	2 cut(s)	1404, 2162
AvaII	GGWCC	5 cut(s)	1675, 1846, 1870, 1994, 3538
AvrII	CCTAGG	1 cut(s)	1595
AxyI	CCTNAGG	1 cut(s)	255
BaeGI	GKGCMC	2 cut(s)	2850, 3436
BalI	TGGCCA	1 cut(s)	1655
BanI	GGYRCC	3 cut(s)	1620, 1774, 3644
BanII	GRGCYC	2 cut(s)	3396, 3507
BauI	CACGAG	1 cut(s)	2724
BbsI	GAAGAC	4 cut(s)	1055, 1827, 2616, 2784
Bbv12I	GWGCWC	5 cut(s)	124, 1731, 3126, 3396, 3436
BceAI	ACGGC	2 cut(s)	1502, 3509
BciT130I	CCWGG	2 cut(s)	2255, 2793
BciVI	GTATCC	2 cut(s)	715, 3054
BcnI	CCSGG	3 cut(s)	1738, 2163, 2164
BcoDI	GTCTC	8 cut(s)	686, 846, 2301, 2686, 3197, 3578, 3692, 3697
BfmI	CTRYAG	4 cut(s)	696, 711, 1323, 3353
BfoI	RGCGCY	1 cut(s)	1277
BfuAI	ACCTGC	2 cut(s)	1026, 3360
BfuI	GTATCC	2 cut(s)	715, 3054
BglII	AGATCT	2 cut(s)	2515, 3448
BlnI	CCTAGG	1 cut(s)	1595
BlpI	GCTNAGC	1 cut(s)	1467
Bme1390I	CCNGG	5 cut(s)	1738, 2163, 2164, 2255, 2793
Bme18I	GGWCC	5 cut(s)	1675, 1846, 1870, 1994, 3538
BmeT110I	CYCGRG	2 cut(s)	1404, 2162
BmgBI	CACGTC	1 cut(s)	662
BmgT120I	GGNCC	7 cut(s)	1675, 1846, 1870, 1994, 2749, 3077, 3538
BmrFI	CCNGG	5 cut(s)	1738, 2163, 2164, 2255, 2793
BmsI	GCATC	6 cut(s)	314, 412, 1791, 2540, 2680, 2765
BpiI	GAAGAC	4 cut(s)	1055, 1827, 2616, 2784
BplI	GAGNNNNNCTC	4 cut(s)	828, 860, 2203, 2235
BpmI	CTGGAG	3 cut(s)	789, 2298, 3069
Bpu1102I	GCTNAGC	1 cut(s)	1467
BpuEI	CTTGAG	1 cut(s)	1430
BpuMI	CCSGG	3 cut(s)	1738, 2163, 2164
BsaAI	YACGTR	2 cut(s)	397, 1241
BsaJI	CCNNGG	8 cut(s)	225, 1168, 1210, 1595, 2162, 2535, 2948, 3298
BsaWI	WCCGGW	3 cut(s)	2579, 2893, 3197
BsaXI	ACNNNNNCTCC	2 cut(s)	2009, 2039
Bsc4I	CCNNNNNNNGG	7 cut(s)	69, 360, 1295, 2260, 2423, 3072, 3686
Bse118I	RCCGGY	1 cut(s)	3197
Bse1I	ACTGG	4 cut(s)	1289, 2133, 2716, 3648
Bse21I	CCTNAGG	1 cut(s)	255
Bse3DI	GCAATG	4 cut(s)	2436, 2880, 3104, 3126
BseBI	CCWGG	2 cut(s)	2255, 2793
BseDI	CCNNGG	8 cut(s)	225, 1168, 1210, 1595, 2162, 2535, 2948, 3298
BseLI	CCNNNNNNNGG	7 cut(s)	69, 360, 1295, 2260, 2423, 3072, 3686
BseMI	GCAATG	4 cut(s)	2436, 2880, 3104, 3126
BseMII	CTCAG	4 cut(s)	881, 1458, 2697, 3687
BseNI	ACTGG	4 cut(s)	1289, 2133, 2716, 3648
BseRI	GAGGAG	5 cut(s)	855, 996, 2355, 2841, 3404
BseSI	GKGCMC	2 cut(s)	2850, 3436
BseYI	CCCAGC	3 cut(s)	979, 2843, 2882
BsgI	GTGCAG	2 cut(s)	852, 3039
Bsh1236I	CGCG	1 cut(s)	3417
BshNI	GGYRCC	3 cut(s)	1620, 1774, 3644
BshTI	ACCGGT	1 cut(s)	3197
BsiHKAI	GWGCWC	5 cut(s)	124, 1731, 3126, 3396, 3436
BsiHKCI	CYCGRG	2 cut(s)	1404, 2162
BsiSI	CCGG	5 cut(s)	1738, 2163, 2580, 2894, 3198
BslFI	GGGAC	6 cut(s)	55, 814, 1426, 2179, 3041, 3524
BslI	CCNNNNNNNGG	7 cut(s)	69, 360, 1295, 2260, 2423, 3072, 3686
BsmAI	GTCTC	8 cut(s)	686, 846, 2301, 2686, 3197, 3578, 3692, 3697
BsmFI	GGGAC	6 cut(s)	55, 814, 1426, 2179, 3041, 3524
BsmI	GAATGC	4 cut(s)	28, 305, 562, 2672
BsoBI	CYCGRG	2 cut(s)	1404, 2162
Bsp1286I	GDGCHC	7 cut(s)	124, 1731, 2850, 3126, 3396, 3436, 3507
Bsp1407I	TGTACA	2 cut(s)	1611, 2666
Bsp1720I	GCTNAGC	1 cut(s)	1467
Bsp19I	CCATGG	2 cut(s)	1168, 1210
Bsp68I	TCGCGA	1 cut(s)	3417
BspACI	CCGC	7 cut(s)	350, 877, 2376, 2441, 3043, 3272, 3501
BspCNI	CTCAG	4 cut(s)	880, 1459, 2696, 3688
BspFNI	CGCG	1 cut(s)	3417
BspHI	TCATGA	1 cut(s)	2065
BspMAI	CTGCAG	2 cut(s)	715, 3357
BspMI	ACCTGC	2 cut(s)	1026, 3360
BspPI	GGATC	5 cut(s)	276, 775, 1153, 1423, 2885
BspT107I	GGYRCC	3 cut(s)	1620, 1774, 3644
BsrBI	CCGCTC	2 cut(s)	877, 3043
BsrDI	GCAATG	4 cut(s)	2436, 2880, 3104, 3126
BsrFI	RCCGGY	1 cut(s)	3197
BsrGI	TGTACA	2 cut(s)	1611, 2666
BsrI	ACTGG	4 cut(s)	1289, 2133, 2716, 3648
BssAI	RCCGGY	1 cut(s)	3197
BssECI	CCNNGG	8 cut(s)	225, 1168, 1210, 1595, 2162, 2535, 2948, 3298
BssSI	CACGAG	1 cut(s)	2724
BssT1I	CCWWGG	4 cut(s)	1168, 1210, 1595, 2535
Bst2BI	CACGAG	1 cut(s)	2724
Bst2UI	CCWGG	2 cut(s)	2255, 2793
Bst6I	CTCTTC	4 cut(s)	1099, 1368, 1857, 2389
BstAUI	TGTACA	2 cut(s)	1611, 2666
BstBAI	YACGTR	2 cut(s)	397, 1241
BstDEI	CTNAG	9 cut(s)	125, 255, 313, 867, 1467, 2369, 2614, 2683, 3696
BstDSI	CCRYGG	4 cut(s)	225, 1168, 1210, 3298
BstENI	CCTNNNNNAGG	1 cut(s)	2421
BstFNI	CGCG	1 cut(s)	3417
BstH2I	RGCGCY	1 cut(s)	1277
BstHHI	GCGC	2 cut(s)	108, 1276
BstMAI	GTCTC	8 cut(s)	686, 846, 2301, 2686, 3197, 3578, 3692, 3697
BstMWI	GCNNNNNNNGC	9 cut(s)	23, 194, 1018, 1472, 2360, 2537, 2879, 3053, 3666
BstNI	CCWGG	2 cut(s)	2255, 2793
BstNSI	RCATGY	2 cut(s)	1891, 3611
BstSCI	CCNGG	5 cut(s)	1736, 2161, 2162, 2253, 2791
BstSFI	CTRYAG	4 cut(s)	696, 711, 1323, 3353
BstSLI	GKGCMC	2 cut(s)	2850, 3436
BstSNI	TACGTA	1 cut(s)	397
BstUI	CGCG	1 cut(s)	3417
BstV2I	GAAGAC	4 cut(s)	1055, 1827, 2616, 2784
BstX2I	RGATCY	4 cut(s)	268, 2515, 2890, 3448
BstYI	RGATCY	4 cut(s)	268, 2515, 2890, 3448
Bsu36I	CCTNAGG	1 cut(s)	255
BsuI	GTATCC	2 cut(s)	715, 3054
BtgI	CCRYGG	4 cut(s)	225, 1168, 1210, 3298
BtrI	CACGTC	1 cut(s)	662
BtsIMutI	CAGTG	9 cut(s)	769, 973, 1504, 1614, 1822, 2676, 2691, 3372, 3655
BtuMI	TCGCGA	1 cut(s)	3417
BveI	ACCTGC	2 cut(s)	1026, 3360
CciI	TCATGA	1 cut(s)	2065
CfoI	GCGC	2 cut(s)	108, 1276
Cfr10I	RCCGGY	1 cut(s)	3197
Cfr13I	GGNCC	7 cut(s)	1675, 1846, 1870, 1994, 2749, 3077, 3538
Cfr9I	CCCGGG	1 cut(s)	2162
CseI	GACGC	1 cut(s)	2336
CsiI	ACCWGGT	1 cut(s)	2791
CspAI	ACCGGT	1 cut(s)	3197
DdeI	CTNAG	9 cut(s)	125, 255, 313, 867, 1467, 2369, 2614, 2683, 3696
DraI	TTTAAA	1 cut(s)	429
DraIII	CACNNNGTG	1 cut(s)	3072
EaeI	YGGCCR	5 cut(s)	351, 1343, 1653, 3216, 3522
Eam1104I	CTCTTC	4 cut(s)	1099, 1368, 1857, 2389
EarI	CTCTTC	4 cut(s)	1099, 1368, 1857, 2389
Ecl136II	GAGCTC	1 cut(s)	3394
Eco105I	TACGTA	1 cut(s)	397
Eco130I	CCWWGG	4 cut(s)	1168, 1210, 1595, 2535
Eco24I	GRGCYC	2 cut(s)	3396, 3507
Eco32I	GATATC	1 cut(s)	2274
Eco47I	GGWCC	5 cut(s)	1675, 1846, 1870, 1994, 3538
Eco47III	AGCGCT	1 cut(s)	1275
Eco53kI	GAGCTC	1 cut(s)	3394
Eco57I	CTGAAG	2 cut(s)	2796, 2844
Eco81I	CCTNAGG	1 cut(s)	255
Eco88I	CYCGRG	2 cut(s)	1404, 2162
EcoICRI	GAGCTC	1 cut(s)	3394
EcoNI	CCTNNNNNAGG	1 cut(s)	2421
EcoO109I	RGGNCCY	2 cut(s)	1994, 3538
EcoRI	GAATTC	1 cut(s)	1109
EcoRII	CCWGG	2 cut(s)	2253, 2791
EcoRV	GATATC	1 cut(s)	2274
EcoT14I	CCWWGG	4 cut(s)	1168, 1210, 1595, 2535
EcoT38I	GRGCYC	2 cut(s)	3396, 3507
ErhI	CCWWGG	4 cut(s)	1168, 1210, 1595, 2535
FaqI	GGGAC	6 cut(s)	55, 814, 1426, 2179, 3041, 3524
FauI	CCCGC	4 cut(s)	870, 3036, 3265, 3494
FriOI	GRGCYC	2 cut(s)	3396, 3507
GlaI	GCGC	2 cut(s)	107, 1275
GsaI	CCCAGC	3 cut(s)	983, 2847, 2886
GsuI	CTGGAG	3 cut(s)	789, 2298, 3069
HaeII	RGCGCY	1 cut(s)	1277
HapII	CCGG	5 cut(s)	1738, 2163, 2580, 2894, 3198
HgaI	GACGC	1 cut(s)	2336
HhaI	GCGC	2 cut(s)	108, 1276
Hin6I	GCGC	2 cut(s)	106, 1274
HinP1I	GCGC	2 cut(s)	106, 1274
HindIII	AAGCTT	2 cut(s)	1083, 2856
HinfI	GANTC	9 cut(s)	490, 802, 839, 1076, 1537, 1959, 2218, 2765, 3410
HpaII	CCGG	5 cut(s)	1738, 2163, 2580, 2894, 3198
Hpy166II	GTNNAC	6 cut(s)	8, 115, 1612, 1918, 3302, 3434
Hpy8I	GTNNAC	6 cut(s)	8, 115, 1612, 1918, 3302, 3434
HpyCH4IV	ACGT	5 cut(s)	85, 396, 661, 1240, 1381
HpyF10VI	GCNNNNNNNGC	9 cut(s)	23, 194, 1018, 1472, 2360, 2537, 2879, 3053, 3666
HpyF3I	CTNAG	9 cut(s)	125, 255, 313, 867, 1467, 2369, 2614, 2683, 3696
HpySE526I	ACGT	5 cut(s)	85, 396, 661, 1240, 1381
HspAI	GCGC	2 cut(s)	106, 1274
KflI	GGGWCCC	1 cut(s)	3538
KpnI	GGTACC	1 cut(s)	1778
LweI	GCATC	6 cut(s)	314, 412, 1791, 2540, 2680, 2765
MabI	ACCWGGT	1 cut(s)	2791
MaeII	ACGT	5 cut(s)	85, 396, 661, 1240, 1381
MaeIII	GTNAC	6 cut(s)	570, 1291, 2622, 2641, 3200, 3368
MbiI	CCGCTC	2 cut(s)	877, 3043
MfeI	CAATTG	1 cut(s)	2149
MflI	RGATCY	4 cut(s)	268, 2515, 2890, 3448
MhlI	GDGCHC	7 cut(s)	124, 1731, 2850, 3126, 3396, 3436, 3507
MlsI	TGGCCA	1 cut(s)	1655
MluNI	TGGCCA	1 cut(s)	1655
MlyI	GAGTC	5 cut(s)	499, 796, 848, 1531, 2212
MmeI	TCCRAC	5 cut(s)	777, 852, 1025, 1123, 1671
Mox20I	TGGCCA	1 cut(s)	1655
MroXI	GAANNNNTTC	2 cut(s)	1390, 2457
MscI	TGGCCA	1 cut(s)	1655
MseI	TTAA	8 cut(s)	153, 428, 459, 497, 728, 1359, 2175, 3138
MslI	CAYNNNNRTG	7 cut(s)	209, 1607, 1626, 2304, 3131, 3612, 3639
Msp20I	TGGCCA	1 cut(s)	1655
MspA1I	CMGCKG	3 cut(s)	2354, 3241, 3272
MspI	CCGG	5 cut(s)	1738, 2163, 2580, 2894, 3198
MspR9I	CCNGG	5 cut(s)	1738, 2163, 2164, 2255, 2793
MunI	CAATTG	1 cut(s)	2149
Mva1269I	GAATGC	4 cut(s)	28, 305, 562, 2672
MvaI	CCWGG	2 cut(s)	2255, 2793
MvnI	CGCG	1 cut(s)	3417
MwoI	GCNNNNNNNGC	9 cut(s)	23, 194, 1018, 1472, 2360, 2537, 2879, 3053, 3666
NciI	CCSGG	3 cut(s)	1738, 2163, 2164
NcoI	CCATGG	2 cut(s)	1168, 1210
NmuCI	GTSAC	4 cut(s)	570, 2641, 3200, 3368
NruI	TCGCGA	1 cut(s)	3417
NspI	RCATGY	2 cut(s)	1891, 3611
PaeI	GCATGC	1 cut(s)	1891
PaeR7I	CTCGAG	1 cut(s)	1404
PagI	TCATGA	1 cut(s)	2065
PaqCI	CACCTGC	1 cut(s)	1026
PcsI	WCGNNNNNNNCGW	1 cut(s)	2400
PctI	GAATGC	4 cut(s)	28, 305, 562, 2672
PdmI	GAANNNNTTC	2 cut(s)	1390, 2457
PfeI	GAWTC	4 cut(s)	1076, 1959, 2765, 3410
PflFI	GACNNNGTC	1 cut(s)	2793
PflMI	CCANNNNNTGG	3 cut(s)	360, 3072, 3686
PinAI	ACCGGT	1 cut(s)	3197
PleI	GAGTC	5 cut(s)	498, 796, 847, 1531, 2212
PpsI	GAGTC	5 cut(s)	498, 796, 847, 1531, 2212
Ppu21I	YACGTR	2 cut(s)	397, 1241
PpuMI	RGGWCCY	2 cut(s)	1994, 3538
PsiI	TTATAA	2 cut(s)	2522, 3348
Psp124BI	GAGCTC	1 cut(s)	3396
Psp5II	RGGWCCY	2 cut(s)	1994, 3538
Psp6I	CCWGG	2 cut(s)	2253, 2791
PspFI	CCCAGC	3 cut(s)	979, 2843, 2882
PspGI	CCWGG	2 cut(s)	2253, 2791
PspPI	GGNCC	7 cut(s)	1675, 1846, 1870, 1994, 2749, 3077, 3538
PspPPI	RGGWCCY	2 cut(s)	1994, 3538
PstI	CTGCAG	2 cut(s)	715, 3357
PsuI	RGATCY	4 cut(s)	268, 2515, 2890, 3448
PsyI	GACNNNGTC	1 cut(s)	2793
PvuII	CAGCTG	2 cut(s)	2354, 3241
RruI	TCGCGA	1 cut(s)	3417
RseI	CAYNNNNRTG	7 cut(s)	209, 1607, 1626, 2304, 3131, 3612, 3639
SacI	GAGCTC	1 cut(s)	3396
SaqAI	TTAA	8 cut(s)	153, 428, 459, 497, 728, 1359, 2175, 3138
Sau96I	GGNCC	7 cut(s)	1675, 1846, 1870, 1994, 2749, 3077, 3538
SbfI	CCTGCAGG	1 cut(s)	3357
SchI	GAGTC	5 cut(s)	499, 796, 848, 1531, 2212
ScrFI	CCNGG	5 cut(s)	1738, 2163, 2164, 2255, 2793
SdaI	CCTGCAGG	1 cut(s)	3357
SduI	GDGCHC	7 cut(s)	124, 1731, 2850, 3126, 3396, 3436, 3507
SexAI	ACCWGGT	1 cut(s)	2791
SfaNI	GCATC	6 cut(s)	314, 412, 1791, 2540, 2680, 2765
SfcI	CTRYAG	4 cut(s)	696, 711, 1323, 3353
Sfr274I	CTCGAG	1 cut(s)	1404
SgrAI	CRCCGGYG	1 cut(s)	3197
SinI	GGWCC	5 cut(s)	1675, 1846, 1870, 1994, 3538
SlaI	CTCGAG	1 cut(s)	1404
SmaI	CCCGGG	1 cut(s)	2164
SmiMI	CAYNNNNRTG	7 cut(s)	209, 1607, 1626, 2304, 3131, 3612, 3639
SmlI	CTYRAG	2 cut(s)	1404, 1445
SmoI	CTYRAG	2 cut(s)	1404, 1445
SnaBI	TACGTA	1 cut(s)	397
SphI	GCATGC	1 cut(s)	1891
Sse8387I	CCTGCAGG	1 cut(s)	3357
SsiI	CCGC	7 cut(s)	350, 877, 2376, 2441, 3043, 3272, 3501
SspI	AATATT	1 cut(s)	55
SstI	GAGCTC	1 cut(s)	3396
StyD4I	CCNGG	5 cut(s)	1736, 2161, 2162, 2253, 2791
StyI	CCWWGG	4 cut(s)	1168, 1210, 1595, 2535
TaiI	ACGT	5 cut(s)	88, 399, 664, 1243, 1384
TaqI	TCGA	2 cut(s)	1405, 2009
TatI	WGTACW	4 cut(s)	1611, 2666, 2814, 3178
TauI	GCSGC	1 cut(s)	353
TfiI	GAWTC	4 cut(s)	1076, 1959, 2765, 3410
Tru1I	TTAA	8 cut(s)	153, 428, 459, 497, 728, 1359, 2175, 3138
Tru9I	TTAA	8 cut(s)	153, 428, 459, 497, 728, 1359, 2175, 3138
TscAI	CASTG	9 cut(s)	769, 973, 1504, 1614, 1822, 2683, 2691, 3379, 3655
TseFI	GTSAC	4 cut(s)	570, 2641, 3200, 3368
Tsp45I	GTSAC	4 cut(s)	570, 2641, 3200, 3368
TspGWI	ACGGA	6 cut(s)	283, 1496, 2589, 2622, 3073, 3287
TspMI	CCCGGG	1 cut(s)	2162
TspRI	CASTG	9 cut(s)	769, 973, 1504, 1614, 1822, 2683, 2691, 3379, 3655
Tth111I	GACNNNGTC	1 cut(s)	2793
Van91I	CCANNNNNTGG	3 cut(s)	360, 3072, 3686
VneI	GTGCAC	1 cut(s)	3432
VpaK11BI	GGWCC	5 cut(s)	1675, 1846, 1870, 1994, 3538
XagI	CCTNNNNNAGG	1 cut(s)	2421
XapI	RAATTY	4 cut(s)	282, 1109, 1387, 2960
XbaI	TCTAGA	1 cut(s)	2407
XceI	RCATGY	2 cut(s)	1891, 3611
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1208
XhoI	CTCGAG	1 cut(s)	1404
XmaI	CCCGGG	1 cut(s)	2162
XmaJI	CCTAGG	1 cut(s)	1595
XmnI	GAANNNNTTC	2 cut(s)	1390, 2457

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.