Rorug02G0425300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0011409
Physical Location & Seq
Reverse (-)
54648566 .. 54649331
766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0425300.1

Sequence Viewer

Length: 522 bp
ATGGATCATTATGGATGTGTCTATAACGAGATCAACGAAAAGGAGAAGCGCTTCCAGATATTCAAGGAAAATGTAGCATTTGTAGAATCATCCAACAATGATGCAAGCAAACCTTACAAATTAAGTGTCAATCAATTTGCAGACCTTACAAATGAAGAATTCATTGCCTCTAGAAATCGATTCAAGGGGCATGAATGTTCCACAAAGACCACATCTTTCAAGTATGAAAACGTTACTGTGCCAGCTACTGTGGACTGGAGACAGAAAGGAGCTGTAACCCCAATCAAGGATCAAGGCCAATGTGGTACAACTTTAATTTCGTCGAAGACGGCGAAGAGCTGTGGTGTGGGAGATGTCGAGGAGCTGGACGTATGGGGTTGTCCTGCAGCGCCTCCGCTACGACGGTGGCTTCTTGCCTCGTCGGACGGGCCATGGTGTGGTGATGCACTGACCGGTTTTCTTTTCTTCTCAGATGGTGGTGATGGCTGCTGTGAGGTTTATCTTTGGGTGGTCGGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

19.13

Weight (kDa)

5.08

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 1 - 54 3.8e-17 Cathepsin propeptide inhibitor domain (I29)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 437
AciI CCGC 1 cut(s) 395
AclI AACGTT 1 cut(s) 231
AclWI GGATC 2 cut(s) 12, 297
AcsI RAATTY 1 cut(s) 158
AfaI GTAC 1 cut(s) 307
AfeI AGCGCT 1 cut(s) 50
AfiI CCNNNNNNNGG 2 cut(s) 286, 437
AgeI ACCGGT 1 cut(s) 452
AgsI TTSAA 3 cut(s) 64, 184, 220
AluBI AGCT 4 cut(s) 245, 272, 339, 364
AluI AGCT 4 cut(s) 245, 272, 339, 364
Alw26I GTCTC 1 cut(s) 253
AlwI GGATC 2 cut(s) 12, 297
AlwNI CAGNNNCTG 1 cut(s) 248
Aor51HI AGCGCT 1 cut(s) 50
AoxI GGCC 2 cut(s) 295, 428
ApeKI GCWGC 2 cut(s) 386, 486
ApoI RAATTY 1 cut(s) 158
ArsI GACNNNNNNTTYG 2 cut(s) 111, 143
AsiGI ACCGGT 1 cut(s) 452
Asp700I GAANNNNTTC 1 cut(s) 50
AspLEI GCGC 2 cut(s) 51, 391
AspS9I GGNCC 1 cut(s) 428
AsuHPI GGTGA 2 cut(s) 452, 491
BbsI GAAGAC 1 cut(s) 332
BbvI GCAGC 2 cut(s) 398, 473
BccI CCATC 2 cut(s) 467, 476
BceAI ACGGC 1 cut(s) 345
BcoDI GTCTC 1 cut(s) 253
BfaI CTAG 1 cut(s) 171
BfmI CTRYAG 1 cut(s) 384
BfoI RGCGCY 2 cut(s) 52, 392
BisI GCNGC 2 cut(s) 387, 487
BlsI GCNGC 2 cut(s) 388, 488
BmgT120I GGNCC 1 cut(s) 428
BmsI GCATC 2 cut(s) 91, 433
BpiI GAAGAC 1 cut(s) 332
BpmI CTGGAG 1 cut(s) 277
Bsa29I ATCGAT 1 cut(s) 178
BsaJI CCNNGG 1 cut(s) 431
BsaWI WCCGGW 1 cut(s) 452
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 437
Bse118I RCCGGY 1 cut(s) 452
Bse1I ACTGG 1 cut(s) 260
Bse3DI GCAATG 1 cut(s) 162
BseCI ATCGAT 1 cut(s) 178
BseDI CCNNGG 1 cut(s) 431
BseGI GGATG 2 cut(s) 20, 89
BseLI CCNNNNNNNGG 2 cut(s) 286, 437
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 1 cut(s) 483
BseNI ACTGG 1 cut(s) 260
BseRI GAGGAG 1 cut(s) 374
BseXI GCAGC 2 cut(s) 398, 473
BshFI GGCC 2 cut(s) 297, 430
BshTI ACCGGT 1 cut(s) 452
BshVI ATCGAT 1 cut(s) 178
BsiSI CCGG 1 cut(s) 453
BslI CCNNNNNNNGG 2 cut(s) 286, 437
BsmAI GTCTC 1 cut(s) 253
BsnI GGCC 2 cut(s) 297, 430
Bsp143I GATC 3 cut(s) 4, 30, 289
Bsp19I CCATGG 1 cut(s) 431
BspACI CCGC 1 cut(s) 395
BspANI GGCC 2 cut(s) 297, 430
BspCNI CTCAG 1 cut(s) 482
BspDI ATCGAT 1 cut(s) 178
BspMAI CTGCAG 1 cut(s) 388
BspPI GGATC 2 cut(s) 12, 297
BspQI GCTCTTC 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 452
BsrI ACTGG 1 cut(s) 260
BssAI RCCGGY 1 cut(s) 452
BssECI CCNNGG 1 cut(s) 431
BssMI GATC 3 cut(s) 4, 30, 289
BssT1I CCWWGG 1 cut(s) 431
Bst4CI ACNGT 3 cut(s) 238, 250, 405
Bst6I CTCTTC 1 cut(s) 329
BstC8I GCNNGC 2 cut(s) 106, 243
BstDEI CTNAG 1 cut(s) 469
BstDSI CCRYGG 1 cut(s) 431
BstF5I GGATG 2 cut(s) 20, 89
BstH2I RGCGCY 2 cut(s) 52, 392
BstHHI GCGC 2 cut(s) 51, 391
BstKTI GATC 3 cut(s) 7, 33, 292
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 3 cut(s) 4, 30, 289
BstSFI CTRYAG 1 cut(s) 384
BstV1I GCAGC 2 cut(s) 398, 473
BstV2I GAAGAC 1 cut(s) 332
Bsu15I ATCGAT 1 cut(s) 178
BsuRI GGCC 2 cut(s) 297, 430
BsuTUI ATCGAT 1 cut(s) 178
BtgI CCRYGG 1 cut(s) 431
BtsCI GGATG 2 cut(s) 20, 89
BtsIMutI CAGTG 1 cut(s) 446
Cac8I GCNNGC 2 cut(s) 106, 243
CaiI CAGNNNCTG 1 cut(s) 248
CfoI GCGC 2 cut(s) 51, 391
Cfr10I RCCGGY 1 cut(s) 452
Cfr13I GGNCC 1 cut(s) 428
ClaI ATCGAT 1 cut(s) 178
Csp6I GTAC 1 cut(s) 306
CspAI ACCGGT 1 cut(s) 452
CviAII CATG 2 cut(s) 191, 432
CviJI RGCY 8 cut(s) 245, 272, 297, 339, 364, 409, 430, 486
CviKI_1 RGCY 8 cut(s) 245, 272, 297, 339, 364, 409, 430, 486
CviQI GTAC 1 cut(s) 306
DdeI CTNAG 1 cut(s) 469
DpnI GATC 3 cut(s) 6, 32, 291
DpnII GATC 3 cut(s) 4, 30, 289
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
Eco130I CCWWGG 1 cut(s) 431
Eco47III AGCGCT 1 cut(s) 50
EcoRI GAATTC 1 cut(s) 158
EcoT14I CCWWGG 1 cut(s) 431
ErhI CCWWGG 1 cut(s) 431
FaeI CATG 2 cut(s) 194, 435
FaiI YATR 6 cut(s) 12, 24, 192, 225, 373, 433
FalI AAGNNNNNCTT 2 cut(s) 97, 129
FatI CATG 2 cut(s) 190, 431
Fnu4HI GCNGC 2 cut(s) 387, 487
FokI GGATG 2 cut(s) 27, 76
Fsp4HI GCNGC 2 cut(s) 387, 487
FspBI CTAG 1 cut(s) 171
GlaI GCGC 2 cut(s) 50, 390
GluI GCNGC 2 cut(s) 387, 487
GsuI CTGGAG 1 cut(s) 277
HaeII RGCGCY 2 cut(s) 52, 392
HaeIII GGCC 2 cut(s) 297, 430
HapII CCGG 1 cut(s) 453
HhaI GCGC 2 cut(s) 51, 391
Hin1II CATG 2 cut(s) 194, 435
Hin6I GCGC 2 cut(s) 49, 389
HinP1I GCGC 2 cut(s) 49, 389
HinfI GANTC 2 cut(s) 86, 180
HpaII CCGG 1 cut(s) 453
HphI GGTGA 2 cut(s) 452, 491
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 3 cut(s) 424, 472, 515
Hpy188III TCNNGA 2 cut(s) 55, 171
Hpy8I GTNNAC 1 cut(s) 253
Hpy99I CGWCG 3 cut(s) 325, 405, 424
HpyCH4III ACNGT 3 cut(s) 238, 250, 405
HpyCH4IV ACGT 2 cut(s) 231, 369
HpyCH4V TGCA 4 cut(s) 104, 140, 386, 446
HpyF3I CTNAG 1 cut(s) 469
HpySE526I ACGT 2 cut(s) 231, 369
Hsp92II CATG 2 cut(s) 194, 435
HspAI GCGC 2 cut(s) 49, 389
Kzo9I GATC 3 cut(s) 4, 30, 289
LguI GCTCTTC 1 cut(s) 329
LmnI GCTCC 2 cut(s) 269, 361
LpnPI CCDG 6 cut(s) 68, 241, 255, 350, 396, 466
Lsp1109I GCAGC 2 cut(s) 398, 473
LweI GCATC 2 cut(s) 91, 433
MaeI CTAG 1 cut(s) 171
MaeII ACGT 2 cut(s) 231, 369
MaeIII GTNAC 2 cut(s) 232, 274
MalI GATC 3 cut(s) 6, 32, 291
MboI GATC 3 cut(s) 4, 30, 289
MboII GAAGA 4 cut(s) 167, 337, 346, 457
MluCI AATT 4 cut(s) 119, 134, 158, 315
MmeI TCCRAC 3 cut(s) 117, 402, 493
MnlI CCTC 6 cut(s) 178, 352, 402, 427, 487, 509
MroXI GAANNNNTTC 1 cut(s) 50
MseI TTAA 2 cut(s) 122, 314
MspI CCGG 1 cut(s) 453
NcoI CCATGG 1 cut(s) 431
NdeII GATC 3 cut(s) 4, 30, 289
NlaIII CATG 2 cut(s) 194, 435
PciSI GCTCTTC 1 cut(s) 329
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 329
PdmI GAANNNNTTC 1 cut(s) 50
PfeI GAWTC 2 cut(s) 86, 180
PflMI CCANNNNNTGG 1 cut(s) 437
PinAI ACCGGT 1 cut(s) 452
PkrI GCNGC 2 cut(s) 388, 488
Psp1406I AACGTT 1 cut(s) 231
PspPI GGNCC 1 cut(s) 428
PstI CTGCAG 1 cut(s) 388
PstNI CAGNNNCTG 1 cut(s) 248
RsaI GTAC 1 cut(s) 307
RsaNI GTAC 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 329
SaqAI TTAA 2 cut(s) 122, 314
SatI GCNGC 2 cut(s) 387, 487
Sau3AI GATC 3 cut(s) 4, 30, 289
Sau96I GGNCC 1 cut(s) 428
SfaNI GCATC 2 cut(s) 91, 433
SfcI CTRYAG 1 cut(s) 384
Sse9I AATT 4 cut(s) 119, 134, 158, 315
SsiI CCGC 1 cut(s) 395
SspMI CTAG 1 cut(s) 171
StyI CCWWGG 1 cut(s) 431
TaaI ACNGT 3 cut(s) 238, 250, 405
TaiI ACGT 2 cut(s) 234, 372
TaqI TCGA 3 cut(s) 178, 323, 357
TasI AATT 4 cut(s) 119, 134, 158, 315
TfiI GAWTC 2 cut(s) 86, 180
Tru1I TTAA 2 cut(s) 122, 314
Tru9I TTAA 2 cut(s) 122, 314
TscAI CASTG 1 cut(s) 453
TseI GCWGC 2 cut(s) 386, 486
TspDTI ATGAA 4 cut(s) 151, 168, 207, 240
TspRI CASTG 1 cut(s) 453
Van91I CCANNNNNTGG 1 cut(s) 437
XapI RAATTY 1 cut(s) 158
XbaI TCTAGA 1 cut(s) 170
XmnI GAANNNNTTC 1 cut(s) 50
XspI CTAG 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.