Rorug03G0045800

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0017685
Physical Location & Seq
Forward (+)
3533980 .. 3535923
1944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0045800.1

Sequence Viewer

Length: 1218 bp
ATGTGTCTAGTAAGTATAGCCGAGAATGAAAGGATTGTTTCTCTTCCTTCCATTGCCAAGACTGAGGGACCCAAATCTTTTGGGGCCAGATTTGCAGCAACTCTTTCTTCACCAAATAATGCTGAGGGACCAGAATCTTCGCTGGCTAGAAATGAAGCAACTCTTTCTCCTCCGAATAAATATTTACAGAATGAAAGGATTGTTTCTCTTCCTTCCATTGCCAAGGCTGAGGGACCCAAATCTTTTGGGGCTAGATTTGCAGCAACTCTTTCTTCACCAAATAATGCTGAGGGACCAGAATCTTCTCTTGCTAGAAATGCAGCAACTCTATCTTCACCGAATAATGCTTTACAGAAAAATCTGTTGGTCACTCTTAAATCATCATCAGACCCTGCAAAACTTGTGTTGGATTCGATGCAAGGGTCTTTGACGTATTACTGGAGAAATGGAGATGTTAGTTCCAAAGAAAAGGTTGTATCTGGTAACATTACTCTATTGAAGATTCTAATGGGACTATCAACACCTGTTGGACCTCATCTGAAAGAAAATGCAACAAATCTAGCATCCCAGTGGAAAGAAAAGTTGAGAGCTGATACTGAAGATTCATTGGAGAGTTTGGGATATTTGCTGTTTATAGCTATGTATGGATTGCTTGGAACCTTACATGAAGATGAGATTGTAATGCTTCTTAGAAGGGTTTCGCAGCATAAGCGGTCTCTGGAGTTATGTCAGACACATGGTTTTGCAGATTATGTCCCTGATTTTATACAGAAGCTTATTGAAAGGAAGCTACTGATGGTGGCTGTTAGATCTATTTATGCCTTCAAATTATTTGACAAGCTCCCCCCAGTGCCACTCTTAAAAGAACATGTGGATGATGTAATGATGTGTTCCGAAGTAATTTACAGGTCACAGATTATACCTGATGAAAAGGATAAGGCTCTGGATGGTAAAATAGCTGATCTCAGAGCTGCCATTCAATGCATCAAAGATTACAACCTCGAGTCTGAATACCCGTCCAAGACTGTTGAATTGCAAATAGTTCAGCTTGATGAAATGCGCAAGGCAAAATGGAGAAGTTTGGAACCATCTCTGGTACCCAAAGTTGAACAAGAAGAGAGAAAAAGGAAGAAGCCTTGTAGTAGCTCTTCTGCCTCAAAGTTACAACCAGGAAAGGTGCTAAAAATAAAATTCGTCTTCAGTGGTTCGAGACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

405

Amino Acids

44.82

Weight (kDa)

9.03

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 114 - 359 9.6e-50 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017685)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g10050
rosa_chinensis RchiOBHm_Chr3g0460751
rosa_laevigata RLG00000024984
rosa_roxburghii Rroxscaffold_6G00419250
rosa_rugosa Rorug03G0045800 Rorug03G0045900 Rorug03G0045900
rosa_samantha Rh3AG103200 Rh3BG106800 Rh3CG108700 Rh3DG108400
rosa_wichuraiana Rw3G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1061
Acc65I GGTACC 1 cut(s) 1096
AccB1I GGYRCC 1 cut(s) 1096
AciI CCGC 1 cut(s) 712
AcsI RAATTY 1 cut(s) 1190
AcuI CTGAAG 2 cut(s) 618, 1183
AfaI GTAC 1 cut(s) 1098
AflIII ACRYGT 1 cut(s) 868
AgsI TTSAA 6 cut(s) 499, 782, 826, 980, 1031, 1109
AjnI CCWGG 1 cut(s) 1168
AjuI GAANNNNNNNTTGG 2 cut(s) 347, 379
AluBI AGCT 9 cut(s) 590, 638, 775, 790, 841, 959, 971, 1048, 1146
AluI AGCT 9 cut(s) 590, 638, 775, 790, 841, 959, 971, 1048, 1146
Alw26I GTCTC 2 cut(s) 720, 1204
AlwNI CAGNNNCTG 1 cut(s) 392
Ama87I CYCGRG 1 cut(s) 1001
AoxI GGCC 1 cut(s) 84
ApeKI GCWGC 5 cut(s) 95, 260, 320, 703, 971
ApoI RAATTY 1 cut(s) 1190
Asp700I GAANNNNTTC 1 cut(s) 697
Asp718I GGTACC 1 cut(s) 1096
AspLEI GCGC 1 cut(s) 1062
AspS9I GGNCC 6 cut(s) 68, 84, 128, 233, 293, 530
AsuHPI GGTGA 3 cut(s) 102, 267, 327
AvaI CYCGRG 1 cut(s) 1001
AvaII GGWCC 5 cut(s) 68, 128, 233, 293, 530
BanI GGYRCC 1 cut(s) 1096
BarI GAAGNNNNNNTAC 2 cut(s) 1132, 1164
BbsI GAAGAC 1 cut(s) 1189
BbvCI CCTCAGC 3 cut(s) 123, 228, 288
BbvI GCAGC 5 cut(s) 107, 272, 332, 715, 958
BccI CCATC 3 cut(s) 790, 941, 1096
BciT130I CCWGG 1 cut(s) 1170
BcoDI GTCTC 2 cut(s) 720, 1204
BfaI CTAG 5 cut(s) 8, 147, 252, 312, 560
BglII AGATCT 1 cut(s) 809
BisI GCNGC 5 cut(s) 96, 261, 321, 704, 972
BlsI GCNGC 5 cut(s) 97, 262, 322, 705, 973
Bme1390I CCNGG 1 cut(s) 1170
Bme18I GGWCC 5 cut(s) 68, 128, 233, 293, 530
BmeT110I CYCGRG 1 cut(s) 1001
BmgT120I GGNCC 6 cut(s) 68, 84, 128, 233, 293, 530
BmrFI CCNGG 1 cut(s) 1170
BmrI ACTGGG 2 cut(s) 562, 842
BmsI GCATC 3 cut(s) 405, 572, 993
BmuI ACTGGG 2 cut(s) 562, 842
BpiI GAAGAC 1 cut(s) 1189
BpmI CTGGAG 2 cut(s) 460, 740
Bpu10I CCTNAGC 3 cut(s) 123, 228, 288
BsaI GGTCTC 2 cut(s) 720, 1204
BsaJI CCNNGG 1 cut(s) 222
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bse1I ACTGG 3 cut(s) 443, 568, 848
Bse3DI GCAATG 2 cut(s) 51, 216
BseBI CCWGG 1 cut(s) 1170
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 3 cut(s) 563, 880, 952
BseMI GCAATG 2 cut(s) 51, 216
BseMII CTCAG 5 cut(s) 54, 114, 219, 279, 979
BseNI ACTGG 3 cut(s) 443, 568, 848
BseRI GAGGAG 1 cut(s) 159
BseXI GCAGC 5 cut(s) 107, 272, 332, 715, 958
BshFI GGCC 1 cut(s) 86
BshNI GGYRCC 1 cut(s) 1096
BsiHKCI CYCGRG 1 cut(s) 1001
BslFI GGGAC 6 cut(s) 81, 141, 246, 306, 525, 740
BsmAI GTCTC 2 cut(s) 720, 1204
BsmFI GGGAC 6 cut(s) 81, 141, 246, 306, 525, 740
BsnI GGCC 1 cut(s) 86
Bso31I GGTCTC 2 cut(s) 720, 1204
BsoBI CYCGRG 1 cut(s) 1001
Bsp143I GATC 2 cut(s) 809, 961
BspACI CCGC 1 cut(s) 712
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 5 cut(s) 55, 115, 220, 280, 978
BspQI GCTCTTC 1 cut(s) 1153
BspT107I GGYRCC 1 cut(s) 1096
BspTNI GGTCTC 2 cut(s) 720, 1204
BsrDI GCAATG 2 cut(s) 51, 216
BsrI ACTGG 3 cut(s) 443, 568, 848
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 2 cut(s) 809, 961
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 1170
Bst4CI ACNGT 1 cut(s) 1027
Bst6I CTCTTC 4 cut(s) 48, 213, 1110, 1153
BstC8I GCNNGC 1 cut(s) 144
BstDEI CTNAG 6 cut(s) 63, 123, 228, 288, 689, 965
BstF5I GGATG 3 cut(s) 563, 880, 952
BstHHI GCGC 1 cut(s) 1062
BstKTI GATC 2 cut(s) 812, 964
BstMAI GTCTC 2 cut(s) 720, 1204
BstMBI GATC 2 cut(s) 809, 961
BstMWI GCNNNNNNNGC 4 cut(s) 92, 257, 317, 709
BstNI CCWGG 1 cut(s) 1170
BstNSI RCATGY 1 cut(s) 872
BstSCI CCNGG 1 cut(s) 1168
BstV1I GCAGC 5 cut(s) 107, 272, 332, 715, 958
BstV2I GAAGAC 1 cut(s) 1189
BstX2I RGATCY 1 cut(s) 809
BstYI RGATCY 1 cut(s) 809
BsuRI GGCC 1 cut(s) 86
BtsCI GGATG 3 cut(s) 563, 880, 952
BtsIMutI CAGTG 3 cut(s) 575, 855, 1207
Cac8I GCNNGC 1 cut(s) 144
CaiI CAGNNNCTG 1 cut(s) 392
CfoI GCGC 1 cut(s) 1062
Cfr13I GGNCC 6 cut(s) 68, 84, 128, 233, 293, 530
Csp6I GTAC 1 cut(s) 1097
CviAII CATG 3 cut(s) 665, 737, 869
CviQI GTAC 1 cut(s) 1097
DdeI CTNAG 6 cut(s) 63, 123, 228, 288, 689, 965
DpnI GATC 2 cut(s) 811, 963
DpnII GATC 2 cut(s) 809, 961
Eam1104I CTCTTC 4 cut(s) 48, 213, 1110, 1153
EarI CTCTTC 4 cut(s) 48, 213, 1110, 1153
Eco130I CCWWGG 1 cut(s) 222
Eco31I GGTCTC 2 cut(s) 720, 1204
Eco47I GGWCC 5 cut(s) 68, 128, 233, 293, 530
Eco57I CTGAAG 2 cut(s) 618, 1183
Eco88I CYCGRG 1 cut(s) 1001
EcoO109I RGGNCCY 2 cut(s) 68, 233
EcoRII CCWGG 1 cut(s) 1168
EcoT14I CCWWGG 1 cut(s) 222
EcoT22I ATGCAT 1 cut(s) 986
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 3 cut(s) 668, 740, 872
FalI AAGNNNNNCTT 2 cut(s) 147, 179
FaqI GGGAC 6 cut(s) 81, 141, 246, 306, 525, 740
FatI CATG 3 cut(s) 664, 736, 868
Fnu4HI GCNGC 5 cut(s) 96, 261, 321, 704, 972
FokI GGATG 3 cut(s) 550, 887, 959
Fsp4HI GCNGC 5 cut(s) 96, 261, 321, 704, 972
FspBI CTAG 5 cut(s) 8, 147, 252, 312, 560
FspI TGCGCA 1 cut(s) 1061
GlaI GCGC 1 cut(s) 1061
GluI GCNGC 5 cut(s) 96, 261, 321, 704, 972
GsuI CTGGAG 2 cut(s) 460, 740
HaeIII GGCC 1 cut(s) 86
HhaI GCGC 1 cut(s) 1062
Hin1II CATG 3 cut(s) 668, 740, 872
Hin6I GCGC 1 cut(s) 1060
HinP1I GCGC 1 cut(s) 1060
HindIII AAGCTT 1 cut(s) 773
HinfI GANTC 6 cut(s) 134, 299, 410, 502, 602, 1004
HphI GGTGA 3 cut(s) 102, 267, 327
Hpy188I TCNGA 7 cut(s) 174, 388, 540, 732, 895, 968, 1009
Hpy188III TCNNGA 3 cut(s) 719, 944, 1209
HpyAV CCTTC 4 cut(s) 57, 222, 687, 832
HpyCH4III ACNGT 1 cut(s) 1027
HpyCH4IV ACGT 1 cut(s) 431
HpyCH4V TGCA 9 cut(s) 95, 260, 320, 395, 418, 551, 746, 984, 1036
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 257, 317, 709
HpyF3I CTNAG 6 cut(s) 63, 123, 228, 288, 689, 965
HpySE526I ACGT 1 cut(s) 431
Hsp92II CATG 3 cut(s) 668, 740, 872
HspAI GCGC 1 cut(s) 1060
KflI GGGWCCC 2 cut(s) 68, 233
KpnI GGTACC 1 cut(s) 1100
Kzo9I GATC 2 cut(s) 809, 961
LguI GCTCTTC 1 cut(s) 1153
LmnI GCTCC 1 cut(s) 846
Lsp1109I GCAGC 5 cut(s) 107, 272, 332, 715, 958
LweI GCATC 3 cut(s) 405, 572, 993
MaeI CTAG 5 cut(s) 8, 147, 252, 312, 560
MaeII ACGT 1 cut(s) 431
MaeIII GTNAC 4 cut(s) 367, 482, 909, 1161
MalI GATC 2 cut(s) 811, 963
MboI GATC 2 cut(s) 809, 961
MflI RGATCY 1 cut(s) 809
MluCI AATT 4 cut(s) 827, 900, 1031, 1190
MlyI GAGTC 1 cut(s) 1013
MmeI TCCRAC 2 cut(s) 387, 508
MnlI CCTC 8 cut(s) 58, 118, 180, 223, 283, 543, 1010, 1165
Mph1103I ATGCAT 1 cut(s) 986
MroXI GAANNNNTTC 1 cut(s) 697
MseI TTAA 3 cut(s) 375, 860, 1216
MslI CAYNNNNRTG 3 cut(s) 568, 669, 873
MspR9I CCNGG 1 cut(s) 1170
MvaI CCWGG 1 cut(s) 1170
MwoI GCNNNNNNNGC 4 cut(s) 92, 257, 317, 709
NdeII GATC 2 cut(s) 809, 961
NlaIII CATG 3 cut(s) 668, 740, 872
NmeAIII GCCGAG 1 cut(s) 46
NmuCI GTSAC 2 cut(s) 367, 909
NsbI TGCGCA 1 cut(s) 1061
NsiI ATGCAT 1 cut(s) 986
NspI RCATGY 1 cut(s) 872
PaeR7I CTCGAG 1 cut(s) 1001
PciI ACATGT 1 cut(s) 868
PciSI GCTCTTC 1 cut(s) 1153
PdmI GAANNNNTTC 1 cut(s) 697
PfeI GAWTC 5 cut(s) 134, 299, 410, 502, 602
PkrI GCNGC 5 cut(s) 97, 262, 322, 705, 973
PleI GAGTC 1 cut(s) 1012
PpsI GAGTC 1 cut(s) 1012
PpuMI RGGWCCY 2 cut(s) 68, 233
PscI ACATGT 1 cut(s) 868
Psp5II RGGWCCY 2 cut(s) 68, 233
Psp6I CCWGG 1 cut(s) 1168
PspGI CCWGG 1 cut(s) 1168
PspPI GGNCC 6 cut(s) 68, 84, 128, 233, 293, 530
PspPPI RGGWCCY 2 cut(s) 68, 233
PspXI VCTCGAGB 1 cut(s) 1001
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 1 cut(s) 809
RsaI GTAC 1 cut(s) 1098
RsaNI GTAC 1 cut(s) 1097
RseI CAYNNNNRTG 3 cut(s) 568, 669, 873
SapI GCTCTTC 1 cut(s) 1153
SaqAI TTAA 3 cut(s) 375, 860, 1216
SatI GCNGC 5 cut(s) 96, 261, 321, 704, 972
Sau3AI GATC 2 cut(s) 809, 961
Sau96I GGNCC 6 cut(s) 68, 84, 128, 233, 293, 530
SchI GAGTC 1 cut(s) 1013
ScrFI CCNGG 1 cut(s) 1170
SfaNI GCATC 3 cut(s) 405, 572, 993
Sfr274I CTCGAG 1 cut(s) 1001
SinI GGWCC 5 cut(s) 68, 128, 233, 293, 530
SlaI CTCGAG 1 cut(s) 1001
SmiMI CAYNNNNRTG 3 cut(s) 568, 669, 873
SmlI CTYRAG 1 cut(s) 1001
SmoI CTYRAG 1 cut(s) 1001
Sse9I AATT 4 cut(s) 827, 900, 1031, 1190
SsiI CCGC 1 cut(s) 712
SspI AATATT 1 cut(s) 182
SspMI CTAG 5 cut(s) 8, 147, 252, 312, 560
StyD4I CCNGG 1 cut(s) 1168
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 1027
TaiI ACGT 1 cut(s) 434
TaqI TCGA 3 cut(s) 413, 1002, 1208
TasI AATT 4 cut(s) 827, 900, 1031, 1190
TfiI GAWTC 5 cut(s) 134, 299, 410, 502, 602
Tru1I TTAA 3 cut(s) 375, 860, 1216
Tru9I TTAA 3 cut(s) 375, 860, 1216
TscAI CASTG 3 cut(s) 575, 855, 1207
TseFI GTSAC 2 cut(s) 367, 909
TseI GCWGC 5 cut(s) 95, 260, 320, 703, 971
Tsp45I GTSAC 2 cut(s) 367, 909
TspDTI ATGAA 7 cut(s) 42, 168, 207, 594, 681, 942, 1068
TspRI CASTG 3 cut(s) 575, 855, 1207
VpaK11BI GGWCC 5 cut(s) 68, 128, 233, 293, 530
XapI RAATTY 1 cut(s) 1190
XceI RCATGY 1 cut(s) 872
XhoI CTCGAG 1 cut(s) 1001
XmnI GAANNNNTTC 1 cut(s) 697
XspI CTAG 5 cut(s) 8, 147, 252, 312, 560
Zsp2I ATGCAT 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.