Rorug03G0123600

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0015279
Physical Location & Seq
Reverse (-)
9983086 .. 9985433
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0123600.1

Sequence Viewer

Length: 1347 bp
ATGACGATGATGAGTCTGGAGACCAATTGTCTTCCAATACATATTAAGTCCGATGATGACAGTGAGGGTGCAAGCAATTATGAGAGGACAAAGAGGCGTAAGGTAGAGGCTGATCAAGGGGGCATTGATCATCATGAGATCAAGGATGCTTCTGCTTCTTGTTTGTGTGATTATTGTGCCAATTTTGCTGACAGATTCGGTTTCCTACATGAGGGAATCTTCAACACACTAAGTGATGAATACATTGAACGTTTACGCAGGCTCAACACTGACAAATTGCTTCGCCAGAGAAAACTCCACCTTGTACTTAATCTAGATCACATCTTTCTGACTTACACAAATCTCAGTGATCTGACCCCAGAAGAAGAAGAATTTTTGGAGACTCTAGCAGATTCGCAGGAAGTTCACAAAGTGCAAATTCCTCCCAAAATGAAACCTATGATTATCAAGTTCAGGCCCTCTCTGAGAAACTTTCTGAAAGAGGCCAGTGGCTTATTCGAAATTTATGCATACACAAATGCTCCTCGACCTCTTGTGTGGAAAATTGTTGAGCTACTGGATCCCAGAAATGAGTACTTCGGTCGTAGAGTAATAACGCCTGACTATCTTTGCAGCACCCACGAAGGAAAGAAGTGTCTTAATCCTGTGCTTGGCCAAGAGTCTGCTGTTTTGGTGGTTGATGACAATCTATGCGGATGGAAGAGGGAAAATCGAAAAAATGTACAGGTAGTTTCAAAGTACAGTTTCTTCAAAAAAAGGAGTGGCCAGAAGAGAAAAAGAAAGTCTCTTTGTGAGTTGAAATCTGATGAAGGGACTGAATGTCTTGTAGTTCTTCTTAGAAATCTTAAACGAATACACAGCAGGTTCTTTGTTGATGAAGTAATGTTCAACAGAGATGTGAGGATGCTGTTAAAAATTGATTGGGACCCTATTTGTGAAGATGGTAGGCTTAAGTTGTGGAAGAGGACATTGTCAAACTGTATGCAGAAAAAAGTTATGGCGTCACTTAGGCAACAGAAGAAGATAGCAGGGCAGTCCTCCAGAACTACTTATGTTAAAGAACAAGTAGATAATCCATCATCGTCAGAGGTTGCAGGAGCAGCTCACGTCGAAAGGTCACAGGCTCGTTGGATCCATCCGAGTTGGATTGACATATCAATCCGTTTATGGCAAAAGGGACATGTGGGCAAGTTTCCTCTTATCCTCAAGGATAGCGAATTTACCTGCAGCTCATGTGGTTGCAGCTCATTTTGGAACACGGGAATCTCCTTGGGCAGTGAAAGGAAACAACATATGTGTCCATCGGAAGAAGCATCAATTGATCCCTCTGTGGAAAGAGAGTCCTGA

Protein Analysis

448

Amino Acids

51.83

Weight (kDa)

8.69

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 100 - 247 1.6e-14 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0015279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36450
fragaria_vesca FvH4_6g18000 FvH4_6g18000 FvH4_6g18000
malus_domestica MD12G1019000.v1.1 MD14G1017000.v1.1
prunus_persica Prupe.7G116300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0472281
rosa_laevigata RLG00000024092
rosa_multiflora Rmu_sc0008192.1_g000020
rosa_roxburghii Rroxscaffold_6G00409100
rosa_rugosa Rorug03G0123600
rosa_samantha Rh3AG175900 Rh3BG201600 Rh3DG173700
rosa_wichuraiana Rw3G016540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 852, 1232
AciI CCGC 1 cut(s) 693
AclI AACGTT 1 cut(s) 250
AclWI GGATC 5 cut(s) 554, 567, 1126, 1139, 1316
AcoI YGGCCR 2 cut(s) 652, 763
AcsI RAATTY 4 cut(s) 371, 417, 501, 1217
AcyI GRCGYC 1 cut(s) 1001
AdeI CACNNNGTG 2 cut(s) 233, 412
AfaI GTAC 4 cut(s) 306, 575, 723, 740
AfiI CCNNNNNNNGG 2 cut(s) 211, 650
AflII CTTAAG 1 cut(s) 950
AflIII ACRYGT 1 cut(s) 1180
AgsI TTSAA 6 cut(s) 223, 248, 735, 751, 799, 889
AhdI GACNNNNNGTC 2 cut(s) 27, 819
AjiI CACGTC 1 cut(s) 1108
AluBI AGCT 4 cut(s) 553, 1103, 1230, 1245
AluI AGCT 4 cut(s) 553, 1103, 1230, 1245
Alw26I GTCTC 3 cut(s) 14, 374, 789
AlwI GGATC 5 cut(s) 554, 567, 1126, 1139, 1316
AoxI GGCC 4 cut(s) 455, 483, 652, 763
ApeKI GCWGC 4 cut(s) 612, 1100, 1227, 1242
ApoI RAATTY 4 cut(s) 371, 417, 501, 1217
AspS9I GGNCC 2 cut(s) 456, 925
AsuII TTCGAA 1 cut(s) 498
AvaII GGWCC 1 cut(s) 925
BalI TGGCCA 2 cut(s) 654, 765
BamHI GGATCC 2 cut(s) 559, 1131
BarI GAAGNNNNNNTAC 2 cut(s) 731, 763
BbsI GAAGAC 1 cut(s) 23
BbvI GCAGC 4 cut(s) 624, 1112, 1239, 1254
BccI CCATC 5 cut(s) 690, 935, 1084, 1143, 1309
BcgI CGANNNNNNTGC 2 cut(s) 488, 522
BclI TGATCA 2 cut(s) 112, 127
BcoDI GTCTC 3 cut(s) 14, 374, 789
BfaI CTAG 2 cut(s) 314, 386
BfmI CTRYAG 1 cut(s) 1225
BfrI CTTAAG 1 cut(s) 950
BfuAI ACCTGC 2 cut(s) 852, 1232
BisI GCNGC 4 cut(s) 613, 1101, 1228, 1243
BlsI GCNGC 4 cut(s) 614, 1102, 1229, 1244
BmcAI AGTACT 1 cut(s) 575
Bme18I GGWCC 1 cut(s) 925
BmeRI GACNNNNNGTC 2 cut(s) 27, 819
BmgBI CACGTC 1 cut(s) 1108
BmgT120I GGNCC 2 cut(s) 456, 925
BmiI GGNNCC 4 cut(s) 561, 926, 927, 1133
BmsI GCATC 3 cut(s) 136, 894, 1322
BpiI GAAGAC 1 cut(s) 23
BpmI CTGGAG 2 cut(s) 38, 1024
Bpu14I TTCGAA 1 cut(s) 498
BpuEI CTTGAG 1 cut(s) 1190
BsaBI GATNNNNATC 1 cut(s) 684
BsaHI GRCGYC 1 cut(s) 1001
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 1 cut(s) 1269
BsaXI ACNNNNNCTCC 2 cut(s) 505, 535
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 650
Bse1I ACTGG 2 cut(s) 486, 561
Bse8I GATNNNNATC 1 cut(s) 684
BseDI CCNNGG 1 cut(s) 1269
BseGI GGATG 4 cut(s) 151, 701, 909, 1135
BseJI GATNNNNATC 1 cut(s) 684
BseLI CCNNNNNNNGG 2 cut(s) 211, 650
BseMII CTCAG 2 cut(s) 358, 455
BseNI ACTGG 2 cut(s) 486, 561
BseRI GAGGAG 1 cut(s) 513
BseXI GCAGC 4 cut(s) 624, 1112, 1239, 1254
Bsh1285I CGRYCG 1 cut(s) 583
BshFI GGCC 4 cut(s) 457, 485, 654, 765
BsiEI CGRYCG 1 cut(s) 583
BslFI GGGAC 3 cut(s) 826, 938, 1191
BslI CCNNNNNNNGG 2 cut(s) 211, 650
BsmAI GTCTC 3 cut(s) 14, 374, 789
BsmFI GGGAC 3 cut(s) 826, 938, 1191
BsnI GGCC 4 cut(s) 457, 485, 654, 765
Bso31I GGTCTC 1 cut(s) 14
Bsp119I TTCGAA 1 cut(s) 498
Bsp1407I TGTACA 1 cut(s) 721
Bsp143I GATC 8 cut(s) 112, 127, 138, 316, 349, 559, 1131, 1321
BspACI CCGC 1 cut(s) 693
BspANI GGCC 4 cut(s) 457, 485, 654, 765
BspCNI CTCAG 2 cut(s) 357, 456
BspHI TCATGA 1 cut(s) 133
BspLI GGNNCC 4 cut(s) 561, 926, 927, 1133
BspMAI CTGCAG 1 cut(s) 1229
BspMI ACCTGC 2 cut(s) 852, 1232
BspPI GGATC 5 cut(s) 554, 567, 1126, 1139, 1316
BspT104I TTCGAA 1 cut(s) 498
BspTI CTTAAG 1 cut(s) 950
BspTNI GGTCTC 1 cut(s) 14
BsrGI TGTACA 1 cut(s) 721
BsrI ACTGG 2 cut(s) 486, 561
BssECI CCNNGG 1 cut(s) 1269
BssMI GATC 8 cut(s) 112, 127, 138, 316, 349, 559, 1131, 1321
BssNI GRCGYC 1 cut(s) 1001
BssT1I CCWWGG 1 cut(s) 1269
Bst4CI ACNGT 3 cut(s) 62, 743, 980
Bst6I CTCTTC 3 cut(s) 695, 764, 956
BstACI GRCGYC 1 cut(s) 1001
BstAFI CTTAAG 1 cut(s) 950
BstAUI TGTACA 1 cut(s) 721
BstBI TTCGAA 1 cut(s) 498
BstC8I GCNNGC 2 cut(s) 73, 260
BstDEI CTNAG 5 cut(s) 230, 344, 464, 836, 1007
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 4 cut(s) 151, 701, 909, 1135
BstKTI GATC 8 cut(s) 115, 130, 141, 319, 352, 562, 1134, 1324
BstMAI GTCTC 3 cut(s) 14, 374, 789
BstMBI GATC 8 cut(s) 112, 127, 138, 316, 349, 559, 1131, 1321
BstMCI CGRYCG 1 cut(s) 583
BstMWI GCNNNNNNNGC 2 cut(s) 185, 1100
BstNSI RCATGY 1 cut(s) 1184
BstSFI CTRYAG 1 cut(s) 1225
BstV1I GCAGC 4 cut(s) 624, 1112, 1239, 1254
BstV2I GAAGAC 1 cut(s) 23
BstX2I RGATCY 2 cut(s) 559, 1131
BstYI RGATCY 2 cut(s) 559, 1131
BsuRI GGCC 4 cut(s) 457, 485, 654, 765
BtrI CACGTC 1 cut(s) 1108
BtsCI GGATG 4 cut(s) 151, 701, 909, 1135
BtsI GCAGTG 1 cut(s) 1282
BtsIMutI CAGTG 5 cut(s) 67, 267, 352, 493, 1282
BveI ACCTGC 2 cut(s) 852, 1232
Cac8I GCNNGC 2 cut(s) 73, 260
CciI TCATGA 1 cut(s) 133
Cfr13I GGNCC 2 cut(s) 456, 925
CseI GACGC 1 cut(s) 990
Csp6I GTAC 4 cut(s) 305, 574, 722, 739
CviAII CATG 4 cut(s) 134, 209, 1181, 1233
CviQI GTAC 4 cut(s) 305, 574, 722, 739
DdeI CTNAG 5 cut(s) 230, 344, 464, 836, 1007
DpnI GATC 8 cut(s) 114, 129, 140, 318, 351, 561, 1133, 1323
DpnII GATC 8 cut(s) 112, 127, 138, 316, 349, 559, 1131, 1321
DraIII CACNNNGTG 2 cut(s) 233, 412
DriI GACNNNNNGTC 2 cut(s) 27, 819
EaeI YGGCCR 2 cut(s) 652, 763
Eam1104I CTCTTC 3 cut(s) 695, 764, 956
Eam1105I GACNNNNNGTC 2 cut(s) 27, 819
EarI CTCTTC 3 cut(s) 695, 764, 956
Eco130I CCWWGG 1 cut(s) 1269
Eco31I GGTCTC 1 cut(s) 14
Eco47I GGWCC 1 cut(s) 925
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoO109I RGGNCCY 2 cut(s) 456, 925
EcoT14I CCWWGG 1 cut(s) 1269
EcoT22I ATGCAT 1 cut(s) 511
ErhI CCWWGG 1 cut(s) 1269
FaeI CATG 4 cut(s) 137, 212, 1184, 1236
FaqI GGGAC 3 cut(s) 826, 938, 1191
FatI CATG 4 cut(s) 133, 208, 1180, 1232
FauNDI CATATG 1 cut(s) 1293
FbaI TGATCA 2 cut(s) 112, 127
Fnu4HI GCNGC 4 cut(s) 613, 1101, 1228, 1243
FokI GGATG 4 cut(s) 158, 708, 916, 1122
Fsp4HI GCNGC 4 cut(s) 613, 1101, 1228, 1243
FspBI CTAG 2 cut(s) 314, 386
GluI GCNGC 4 cut(s) 613, 1101, 1228, 1243
GsuI CTGGAG 2 cut(s) 38, 1024
HaeIII GGCC 4 cut(s) 457, 485, 654, 765
HgaI GACGC 1 cut(s) 990
Hin1I GRCGYC 1 cut(s) 1001
Hin1II CATG 4 cut(s) 137, 212, 1184, 1236
HinfI GANTC 8 cut(s) 13, 195, 216, 382, 392, 659, 1263, 1340
Hpy166II GTNNAC 2 cut(s) 254, 406
Hpy188I TCNGA 9 cut(s) 52, 330, 354, 465, 477, 805, 1087, 1140, 1306
Hpy188III TCNNGA 5 cut(s) 17, 134, 314, 1041, 1344
Hpy8I GTNNAC 2 cut(s) 254, 406
Hpy99I CGWCG 1 cut(s) 1112
HpyAV CCTTC 2 cut(s) 617, 803
HpyCH4III ACNGT 3 cut(s) 62, 743, 980
HpyCH4IV ACGT 2 cut(s) 250, 1107
HpyCH4V TGCA 8 cut(s) 71, 415, 509, 612, 985, 1094, 1227, 1242
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 1100
HpyF3I CTNAG 5 cut(s) 230, 344, 464, 836, 1007
HpySE526I ACGT 2 cut(s) 250, 1107
Hsp92I GRCGYC 1 cut(s) 1001
Hsp92II CATG 4 cut(s) 137, 212, 1184, 1236
KflI GGGWCCC 1 cut(s) 925
Ksp22I TGATCA 2 cut(s) 112, 127
Kzo9I GATC 8 cut(s) 112, 127, 138, 316, 349, 559, 1131, 1321
LmnI GCTCC 2 cut(s) 526, 1097
Lsp1109I GCAGC 4 cut(s) 624, 1112, 1239, 1254
LweI GCATC 3 cut(s) 136, 894, 1322
MaeI CTAG 2 cut(s) 314, 386
MaeII ACGT 2 cut(s) 250, 1107
MaeIII GTNAC 2 cut(s) 1002, 1116
MalI GATC 8 cut(s) 114, 129, 140, 318, 351, 561, 1133, 1323
MboI GATC 8 cut(s) 112, 127, 138, 316, 349, 559, 1131, 1321
MfeI CAATTG 2 cut(s) 25, 1317
MflI RGATCY 2 cut(s) 559, 1131
MlsI TGGCCA 2 cut(s) 654, 765
MluNI TGGCCA 2 cut(s) 654, 765
MlyI GAGTC 3 cut(s) 22, 376, 668
MmeI TCCRAC 2 cut(s) 1109, 1124
Mox20I TGGCCA 2 cut(s) 654, 765
Mph1103I ATGCAT 1 cut(s) 511
MscI TGGCCA 2 cut(s) 654, 765
MseI TTAA 7 cut(s) 45, 309, 639, 846, 911, 951, 1056
Msp20I TGGCCA 2 cut(s) 654, 765
MspCI CTTAAG 1 cut(s) 950
MunI CAATTG 2 cut(s) 25, 1317
MwoI GCNNNNNNNGC 2 cut(s) 185, 1100
NdeI CATATG 1 cut(s) 1293
NdeII GATC 8 cut(s) 112, 127, 138, 316, 349, 559, 1131, 1321
NlaIII CATG 4 cut(s) 137, 212, 1184, 1236
NlaIV GGNNCC 4 cut(s) 561, 926, 927, 1133
NmuCI GTSAC 2 cut(s) 1002, 1116
NsiI ATGCAT 1 cut(s) 511
NspI RCATGY 1 cut(s) 1184
NspV TTCGAA 1 cut(s) 498
PagI TCATGA 1 cut(s) 133
PciI ACATGT 1 cut(s) 1180
PfeI GAWTC 4 cut(s) 195, 216, 392, 1263
PflFI GACNNNGTC 1 cut(s) 970
PkrI GCNGC 4 cut(s) 614, 1102, 1229, 1244
PleI GAGTC 3 cut(s) 21, 376, 667
PpsI GAGTC 3 cut(s) 21, 376, 667
PpuMI RGGWCCY 1 cut(s) 925
PscI ACATGT 1 cut(s) 1180
Psp1406I AACGTT 1 cut(s) 250
Psp5II RGGWCCY 1 cut(s) 925
PspN4I GGNNCC 4 cut(s) 561, 926, 927, 1133
PspPI GGNCC 2 cut(s) 456, 925
PspPPI RGGWCCY 1 cut(s) 925
PstI CTGCAG 1 cut(s) 1229
PsuI RGATCY 2 cut(s) 559, 1131
PsyI GACNNNGTC 1 cut(s) 970
RsaI GTAC 4 cut(s) 306, 575, 723, 740
RsaNI GTAC 4 cut(s) 305, 574, 722, 739
SaqAI TTAA 7 cut(s) 45, 309, 639, 846, 911, 951, 1056
SatI GCNGC 4 cut(s) 613, 1101, 1228, 1243
Sau3AI GATC 8 cut(s) 112, 127, 138, 316, 349, 559, 1131, 1321
Sau96I GGNCC 2 cut(s) 456, 925
ScaI AGTACT 1 cut(s) 575
SchI GAGTC 3 cut(s) 22, 376, 668
SfaNI GCATC 3 cut(s) 136, 894, 1322
SfcI CTRYAG 1 cut(s) 1225
SfuI TTCGAA 1 cut(s) 498
SinI GGWCC 1 cut(s) 925
SmlI CTYRAG 2 cut(s) 950, 1205
SmoI CTYRAG 2 cut(s) 950, 1205
SsiI CCGC 1 cut(s) 693
SspMI CTAG 2 cut(s) 314, 386
StyI CCWWGG 1 cut(s) 1269
TaaI ACNGT 3 cut(s) 62, 743, 980
TaiI ACGT 2 cut(s) 253, 1110
TaqI TCGA 4 cut(s) 498, 526, 712, 1110
TaqII GACCGA 1 cut(s) 569
TatI WGTACW 4 cut(s) 304, 573, 721, 738
TfiI GAWTC 4 cut(s) 195, 216, 392, 1263
Tru1I TTAA 7 cut(s) 45, 309, 639, 846, 911, 951, 1056
Tru9I TTAA 7 cut(s) 45, 309, 639, 846, 911, 951, 1056
TscAI CASTG 5 cut(s) 67, 274, 352, 493, 1282
TseFI GTSAC 2 cut(s) 1002, 1116
TseI GCWGC 4 cut(s) 612, 1100, 1227, 1242
Tsp45I GTSAC 2 cut(s) 1002, 1116
TspDTI ATGAA 4 cut(s) 252, 446, 822, 891
TspGWI ACGGA 1 cut(s) 1151
TspRI CASTG 5 cut(s) 67, 274, 352, 493, 1282
Tth111I GACNNNGTC 1 cut(s) 970
Vha464I CTTAAG 1 cut(s) 950
VpaK11BI GGWCC 1 cut(s) 925
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 4 cut(s) 371, 417, 501, 1217
XbaI TCTAGA 1 cut(s) 313
XceI RCATGY 1 cut(s) 1184
XspI CTAG 2 cut(s) 314, 386
ZrmI AGTACT 1 cut(s) 575
Zsp2I ATGCAT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.