Rorug03G0273200

C-type lectin receptor-like tyrosine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004119
Physical Location & Seq
Reverse (-)
26987109 .. 26995585
8477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0273200.1

Sequence Viewer

Length: 3981 bp
ATGTCGCCGGCGGCGGCGGACGCCCGGTCCCCGATTACCTCTAAACCCTCCGGCAATCCTCAAAACCCTAAACCTTCATCGGAGGAGACGCCTTCCTCTAACCCCTTCAATTTCAATGCTGTTGGTTCCGACGGTCCATCGTGTCCAAATGTGCCGTCACGATCAGCTCGGCCGCGGCCGAGGTTGGCGAAGCAGAGGAGGAAGCAGCAGGCGAATGCGGGGTCGAGAACTGGGCCGGCTGAGACGGCTGGGCCGGAGGTCAACCCGTTTTGCTCGGTAAGTGGTGGTGGTGGTGCTGCTACTAGTGTTTTTAATCAGTTCAGTTTTCCGAGTGGTGGTGTGGGTTTTGTGTTTGGCGCTACATTGAGTGGTGTTGATGAGCGGGCTCAGGTGAATAGTGGGGATGGTAGAGAGTTCGGTGAGGTTGAGAGTGTGAGTGCTAAGCAGGATGATCCTCCGGGACCGAGTTCGTGTGGGGAGAGGAGAGAATGTGACACTGATCAGAGTGGAAAAGAGATATTCGAGTGTGATCAGAACCATTCAGCTCCGAATTTGAGTGCCGAAAATGGAGAATCGGCAGAGGTAGGACGGAGTGAATATTGTGAAAATGAGGGGAAGTCAGGTAATGACTCTAGTGGTATTGGTTTTGTGTTTGGTTCCGGTTGGTTTAATTCAAGTTCAGATTTGAATTCAGGAAAGAATATGGAAAAAGTGGTTGATGAAGTTGGCAGGAAGATGAGAGTAGAGAGTGACACCGAGTTTGAGAAAGTGAAAGTTAATTCTTTCGAATTTCATAGTGAGGAGCGCTGGAGCTCAGACAAGGTTCATGATGAGGGCATTTTTGTATTTGGAAGCAGCGGTAAGAAAGGTTCCAGTTTGATGGATTCCAAGGTAGCTAATTGTCAAGATGAGGTGAAATCAAGTGATGAAGGAGATTGCAAGTCCAATTCAAACAGTCGGAATGATAATCTGAGTTCTGATTTCAGTAGCAAGTGTAATCTTGTATCTGAAACTAGTGAGGTAACTTGTGGAGATAATCATAAAAGTACTGACCATGTTGTATTTGGAAGCGAAAGTAACACGGCTGGTACCACCAGTGGGATTTCTAGTTCCACTGAGTTTACATTTCAGGCAGGACTTGGTGATAGTGTTGATGCAGGCCAGTTTCCTGAGTGTCAAATTAATAAACCCAATGCAGCTGCTGGCCCATGTTCATTCTCATCAATTGGTCTTGGCATCCAACTAAATGGTTGTGTTTCTGAAGCAGCTTCTGCGGGTGGAGTAAAAGGTAAAGATGAAAGTAGCTTTACTAGCTCTCCAGATGGATTTGGAGTGTGCTTTGAGGACTTCAACACTCCATTGCGGTTTCCTGCTTGCCTCAAAGAAAATCTATTTCCTGAAATAAATAAATCAAACATCAGTATGAAGGGTAGATCGGTTAGGGACAAGAGATCAAGGAAAACAAGGGGAAAGTTGAAGAAACCTTCTCTGAGTAAGCAATGGCCAGCACAAGATAATGTTCCCAAGGAAAGTAGTTCCCAAGAAACCCCAGTCTCACCTGGATGTGGCTCACCCATGGATTTCTCTCCATATGAGGAAACTACAGTTGCTAATCCACATTCGAGGGAATCTTCTACAGTGTCTAATGACACCTTCCACCTAGTCAGTAATACTGCACCTTGTGGCTCCGATGCAACAGTTCCTATTGGACCTAATGGTGATGAGGGAGAATTTGCCGCTAAAGGTGATCAGATATATGGTAAGGCCACTGACGAGAATTCTAGGTATCATGGTGAAAGAATATTTGTTGACAATTGTTCCTCCAAAGGGTCTGGTTCTGGAGCTGAAACTCCCTGCTTTAGCTCCAATGGTATCAGTCATGCTGGTAAGGATTCAGAAGACGTTAGAGCTGATAATGGTGTGAATTTAGAAAGTCAAGAACAGGATTGTGGAACACCGTGTTTTGCTTCTGGTTTGGAAAATATGGAGAGGAAAACATTTACCTTTGTTGCATCATCTTCTGCAGAAGGAAGTCCAATAGTGGCAAGGCGTCTTCTGCGCAGAGCGAAAAATAAGAAGAAAGTTGGTTCTAATACGTTTGTTATAACTCCAAGTCCAAATGTTCAGTTTGGAGGTTCTGACCAATTTACACCCCACTCTAGCACACCATTGTCTTTTGATGCGGTAGGAAAATCTGAGCCCCATGAACAAGTACCGAAAGTGCATATCCCTTCTACTGAGGCTACTCATGAGACTTGTGAGAAGTGGCGAATTAGGGGCAACAAAGCTTATAGGAACAAAGACTTTTCAAAAGCTGAAGACTTTTACACACAGGGAATAGTTTCTGTACCTTCAAGTGAAAGATCGGGAAGCTGCCTTAAGCCTCTTTTACTATGCTATAGCAATCGTGCAGCAACAAGGATGTATATGGGAAGGATAAGGGAAGCTTTAGGGGACTGCATGATGGCCACTGCACTAGACCCCAACTTTCTCAAAGTTCAAATGAGAGCTGCAAATTTCCATCTTCTTCTCGGCGAAGTTGAAAATGCCCAACGGTACTTTAATAATTGCTTGGAATCCGGAACTGGTGTGTGTTTGGATCGGAGAATTGTAATTGATTCTGCTGATGGCATACAAAAGGCTCAGAAAGTGGCTGAGTATACGAATCGCTCTGCCAAGCTTCTGGAACAGAGAACTACAGATGCAGCTCTCAGTGCCTTGGAAATTGTTTCTGAGGCCTTGTCTATTAGTGAATACTCGGAAAAGTTACTTGAAATGAAGGCAGAGGCTTTGTGTTTGTTGAGGAGATATGAAGAGGCAATTCAACTGTGTGAACAGAGTATGTATTTTGCTGAGAAGAACTTTTCTTTGCTGAACAGTGTAGTAAATATGGATACTGCTGGATGTGAAGATCATCCATATGCTAGGTTGTGGAGGTGGTTCTTCATATCCAAATCTTACTTTCATTTGGGAAGGTTTCAGGCAGCTCTTGATTTACTTGAGAAGCTAGAGCAAGTGGGATCTATCAAAGACAGGTATGCGAGTAAGAATCTGGAATCATCAATTTCATTAGCTGTCACCATACGTGAACTGTTAAGCCACAAGGATGCAGGGAATGAAGCATTTAGGTCAGGAAGATATACAGAAGCAGTGGAGCATTATACTGTTGCTTTATCTCGCAATATTGGATCTCGGCCATTTTCTTCAATCTGTTTGTGCAACCGTGCTGCAGCACACCAAGCTTTGGGTCAAATTACTGATGCCATTGCTGACTGCAGTCTTGCAATAGCTCTTGATGGACATTATGTTAAGGCAGTTTCTAGAAGAGCTACCTTACATGAGATGATACGGGACTATGGACAGGCTGCTAGCGATCTCCAAAGACTTATTTCTGTACTGGAAAACCAATCTGGTGACAAGGCCAAAGAACCTACCTCACAAGGTAGCTCCAATGGCCGTACACAAGAATTACGGAACTCTTATCGACGTATGCCTTTAATGGAAGAAGAAGCTAAGAAAGGAATCCCCTTGAATTTTTACCTCATTCTGGGAATTAAATCATCTGACACAAGTTCTGACATTAAGAAGGCATACCGTAAGGCTGCTCTCAAGCATCATCCCGACAAGGCTGGTCAGTTCTTGGCAAGAAGTGAGAGTGGAGATGAAGGGCAACTCTGGAAAGAAATCTCTCTCGAGGTTCACAAGGATGCTGACAGACTCTTTAAAATGATCGGAGAGGCATATGCAGTACTTTCAGATCCCAACAAGCGCTCAGAATATGATCTTGAGGAAGACATAAGAAAATCAGCAAAGGAAAGCAAGGAACGTGGCGTCTACAGAAAGTCTTCAGATTTTCAGAGTCCTAGACGCAGCAGCTATAGAAAACCAGATTTTTATAGCTCTCCATTCGAGAGAAGCTCATATTGTCGGAACAGTAGGGAGGGTTGGAAGACATATGGAGATTCGTATTCACGATGGTAA

Protein Analysis

1326

Amino Acids

144.59

Weight (kDa)

5.93

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 1178 - 1210 1.8e-09 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2105
Acc16I TGCGCA 1 cut(s) 2060
Acc65I GGTACC 1 cut(s) 1088
AccB1I GGYRCC 1 cut(s) 1088
AccB7I CCANNNNNTGG 1 cut(s) 3244
AccBSI CCGCTC 1 cut(s) 382
AccI GTMKAC 2 cut(s) 2660, 3834
AccII CGCG 1 cut(s) 175
AccIII TCCGGA 1 cut(s) 2579
AclWI GGATC 5 cut(s) 446, 2607, 3028, 3196, 3752
AcoI YGGCCR 6 cut(s) 170, 176, 1502, 2466, 3194, 3454
AcsI RAATTY 8 cut(s) 550, 688, 788, 1730, 1777, 1924, 2515, 3532
AcuI CTGAAG 3 cut(s) 1281, 2337, 3831
AcyI GRCGYC 4 cut(s) 21, 89, 2050, 3831
AdeI CACNNNGTG 1 cut(s) 1682
AfaI GTAC 8 cut(s) 1048, 1090, 2214, 2349, 2558, 3396, 3460, 3750
AfeI AGCGCT 2 cut(s) 806, 3770
AfiI CCNNNNNNNGG 6 cut(s) 335, 1098, 1565, 1827, 3244, 3547
AflII CTTAAG 1 cut(s) 2378
AhdI GACNNNNNGTC 1 cut(s) 25
AhlI ACTAGT 2 cut(s) 302, 1013
AjnI CCWGG 1 cut(s) 1558
AloI GAACNNNNNNTCC 2 cut(s) 1093, 1125
Alw21I GWGCWC 1 cut(s) 815
Alw26I GTCTC 4 cut(s) 80, 236, 1558, 2246
AlwI GGATC 5 cut(s) 446, 2607, 3028, 3196, 3752
AlwNI CAGNNNCTG 2 cut(s) 1202, 1271
Ama87I CYCGRG 1 cut(s) 3692
Aor13HI TCCGGA 1 cut(s) 2579
Aor51HI AGCGCT 2 cut(s) 806, 3770
ApoI RAATTY 8 cut(s) 550, 688, 788, 1730, 1777, 1924, 2515, 3532
ArsI GACNNNNNNTTYG 6 cut(s) 140, 172, 206, 238, 743, 775
AseI ATTAAT 1 cut(s) 1182
Asp700I GAANNNNTTC 2 cut(s) 2341, 3844
Asp718I GGTACC 1 cut(s) 1088
AspLEI GCGC 4 cut(s) 359, 807, 2061, 3771
AspS9I GGNCC 7 cut(s) 27, 134, 233, 251, 461, 1205, 1709
AsuC2I CCSGG 2 cut(s) 25, 459
AsuII TTCGAA 1 cut(s) 786
AsuNHI GCTAGC 1 cut(s) 3368
AvaI CYCGRG 1 cut(s) 3692
AvaII GGWCC 4 cut(s) 27, 134, 461, 1709
BalI TGGCCA 2 cut(s) 1504, 2468
BanI GGYRCC 1 cut(s) 1088
BanII GRGCYC 3 cut(s) 388, 815, 2202
BbsI GAAGAC 6 cut(s) 1905, 2045, 2325, 3798, 3837, 3956
Bbv12I GWGCWC 1 cut(s) 815
BccI CCATC 9 cut(s) 145, 398, 874, 1316, 2458, 2529, 2621, 3290, 3969
BceAI ACGGC 4 cut(s) 139, 261, 1098, 3441
BciT130I CCWGG 1 cut(s) 1560
BciVI GTATCC 1 cut(s) 2887
BclI TGATCA 3 cut(s) 499, 529, 1747
BcnI CCSGG 2 cut(s) 25, 459
BcoDI GTCTC 4 cut(s) 80, 236, 1558, 2246
BcuI ACTAGT 2 cut(s) 302, 1013
BfmI CTRYAG 9 cut(s) 1602, 1635, 2022, 2398, 2697, 3228, 3274, 3835, 3877
BfoI RGCGCY 3 cut(s) 360, 808, 3772
BfrI CTTAAG 1 cut(s) 2378
BfuI GTATCC 1 cut(s) 2887
BlpI GCTNAGC 1 cut(s) 441
BmcAI AGTACT 2 cut(s) 1048, 3750
Bme1390I CCNGG 3 cut(s) 25, 459, 1560
Bme18I GGWCC 4 cut(s) 27, 134, 461, 1709
BmeRI GACNNNNNGTC 1 cut(s) 25
BmeT110I CYCGRG 1 cut(s) 3692
BmgT120I GGNCC 7 cut(s) 27, 134, 233, 251, 461, 1205, 1709
BmiI GGNNCC 7 cut(s) 29, 127, 462, 658, 871, 1090, 1687
BmrFI CCNGG 3 cut(s) 25, 459, 1560
BmrI ACTGGG 2 cut(s) 240, 1544
BmtI GCTAGC 1 cut(s) 3372
BmuI ACTGGG 2 cut(s) 240, 1544
BoxI GACNNNNGTC 1 cut(s) 3276
BpiI GAAGAC 6 cut(s) 1905, 2045, 2325, 3798, 3837, 3956
BplI GAGNNNNNCTC 2 cut(s) 3902, 3934
BpmI CTGGAG 3 cut(s) 829, 1302, 1860
Bpu10I CCTNAGC 1 cut(s) 387
Bpu1102I GCTNAGC 1 cut(s) 441
Bpu14I TTCGAA 1 cut(s) 786
BpuEI CTTGAG 3 cut(s) 3020, 3593, 3806
BpuMI CCSGG 2 cut(s) 25, 459
BsaAI YACGTR 1 cut(s) 3086
BsaHI GRCGYC 4 cut(s) 21, 89, 2050, 3831
BsaJI CCNNGG 6 cut(s) 173, 179, 888, 1524, 1575, 2718
BsaWI WCCGGW 2 cut(s) 659, 2579
BsaXI ACNNNNNCTCC 4 cut(s) 475, 505, 1300, 1330
Bsc4I CCNNNNNNNGG 6 cut(s) 335, 1098, 1565, 1827, 3244, 3547
Bse118I RCCGGY 2 cut(s) 7, 235
Bse1I ACTGG 7 cut(s) 235, 873, 1095, 1162, 1550, 2590, 3402
Bse3DI GCAATG 3 cut(s) 1358, 1505, 3264
BseAI TCCGGA 1 cut(s) 2579
BseBI CCWGG 1 cut(s) 1560
BseDI CCNNGG 6 cut(s) 173, 179, 888, 1524, 1575, 2718
BseLI CCNNNNNNNGG 6 cut(s) 335, 1098, 1565, 1827, 3244, 3547
BseMI GCAATG 3 cut(s) 1358, 1505, 3264
BseNI ACTGG 7 cut(s) 235, 873, 1095, 1162, 1550, 2590, 3402
BseRI GAGGAG 5 cut(s) 98, 211, 496, 815, 2818
BseX3I CGGCCG 2 cut(s) 170, 176
BseYI CCCAGC 1 cut(s) 248
BsgI GTGCAG 3 cut(s) 1659, 2430, 2457
Bsh1236I CGCG 1 cut(s) 175
Bsh1285I CGRYCG 2 cut(s) 173, 179
BshNI GGYRCC 1 cut(s) 1088
BsiEI CGRYCG 2 cut(s) 173, 179
BsiHKAI GWGCWC 1 cut(s) 815
BsiHKCI CYCGRG 1 cut(s) 3692
BsiSI CCGG 8 cut(s) 8, 25, 51, 236, 254, 458, 660, 2580
BslFI GGGAC 5 cut(s) 13, 474, 1457, 2468, 3365
BslI CCNNNNNNNGG 6 cut(s) 335, 1098, 1565, 1827, 3244, 3547
BsmAI GTCTC 4 cut(s) 80, 236, 1558, 2246
BsmBI CGTCTC 2 cut(s) 80, 236
BsmFI GGGAC 5 cut(s) 13, 474, 1457, 2468, 3365
BsmI GAATGC 1 cut(s) 220
BsoBI CYCGRG 1 cut(s) 3692
Bsp119I TTCGAA 1 cut(s) 786
Bsp1286I GDGCHC 3 cut(s) 388, 815, 2202
Bsp13I TCCGGA 1 cut(s) 2579
Bsp1720I GCTNAGC 1 cut(s) 441
Bsp19I CCATGG 1 cut(s) 1575
BspEI TCCGGA 1 cut(s) 2579
BspFNI CGCG 1 cut(s) 175
BspHI TCATGA 2 cut(s) 826, 2248
BspLI GGNNCC 7 cut(s) 29, 127, 462, 658, 871, 1090, 1687
BspMAI CTGCAG 3 cut(s) 2026, 3232, 3278
BspOI GCTAGC 1 cut(s) 3372
BspPI GGATC 5 cut(s) 446, 2607, 3028, 3196, 3752
BspQI GCTCTTC 1 cut(s) 3319
BspT104I TTCGAA 1 cut(s) 786
BspT107I GGYRCC 1 cut(s) 1088
BspTI CTTAAG 1 cut(s) 2378
BsrBI CCGCTC 1 cut(s) 382
BsrDI GCAATG 3 cut(s) 1358, 1505, 3264
BsrFI RCCGGY 2 cut(s) 7, 235
BsrI ACTGG 7 cut(s) 235, 873, 1095, 1162, 1550, 2590, 3402
BssAI RCCGGY 2 cut(s) 7, 235
BssECI CCNNGG 6 cut(s) 173, 179, 888, 1524, 1575, 2718
BssNAI GTATAC 1 cut(s) 2661
BssNI GRCGYC 4 cut(s) 21, 89, 2050, 3831
BssT1I CCWWGG 4 cut(s) 888, 1524, 1575, 2718
Bst1107I GTATAC 1 cut(s) 2661
Bst2UI CCWGG 1 cut(s) 1560
Bst6I CTCTTC 2 cut(s) 2808, 3319
BstACI GRCGYC 4 cut(s) 21, 89, 2050, 3831
BstAFI CTTAAG 1 cut(s) 2378
BstAPI GCANNNNNTGC 1 cut(s) 1271
BstBAI YACGTR 1 cut(s) 3086
BstBI TTCGAA 1 cut(s) 786
BstC8I GCNNGC 9 cut(s) 9, 210, 237, 384, 1159, 1204, 1375, 1506, 3370
BstDSI CCRYGG 2 cut(s) 173, 1575
BstFNI CGCG 1 cut(s) 175
BstH2I RGCGCY 3 cut(s) 360, 808, 3772
BstHHI GCGC 4 cut(s) 359, 807, 2061, 3771
BstMAI GTCTC 4 cut(s) 80, 236, 1558, 2246
BstMCI CGRYCG 2 cut(s) 173, 179
BstMWI GCNNNNNNNGC 8 cut(s) 20, 245, 1271, 1311, 2056, 2714, 3239, 3453
BstNI CCWGG 1 cut(s) 1560
BstPAI GACNNNNGTC 1 cut(s) 3276
BstSCI CCNGG 3 cut(s) 23, 457, 1558
BstSFI CTRYAG 9 cut(s) 1602, 1635, 2022, 2398, 2697, 3228, 3274, 3835, 3877
BstUI CGCG 1 cut(s) 175
BstV2I GAAGAC 6 cut(s) 1905, 2045, 2325, 3798, 3837, 3956
BstX2I RGATCY 3 cut(s) 3020, 3188, 3757
BstXI CCANNNNNNTGG 3 cut(s) 880, 1247, 2683
BstYI RGATCY 3 cut(s) 3020, 3188, 3757
BstZ17I GTATAC 1 cut(s) 2661
BstZI CGGCCG 2 cut(s) 170, 176
BsuI GTATCC 1 cut(s) 2887
BtgI CCRYGG 2 cut(s) 173, 1575
BtsI GCAGTG 2 cut(s) 2469, 3156
BtsIMutI CAGTG 9 cut(s) 495, 1102, 1113, 1644, 1767, 2469, 2719, 2884, 3156
Cac8I GCNNGC 9 cut(s) 9, 210, 237, 384, 1159, 1204, 1375, 1506, 3370
CaiI CAGNNNCTG 2 cut(s) 1202, 1271
CciI TCATGA 2 cut(s) 826, 2248
CfoI GCGC 4 cut(s) 359, 807, 2061, 3771
Cfr10I RCCGGY 2 cut(s) 7, 235
Cfr13I GGNCC 7 cut(s) 27, 134, 233, 251, 461, 1205, 1709
Cfr42I CCGCGG 1 cut(s) 176
CseI GACGC 5 cut(s) 29, 97, 2039, 3820, 3876
Csp6I GTAC 8 cut(s) 1047, 1089, 2213, 2348, 2557, 3395, 3459, 3749
CspCI CAANNNNNGTGG 2 cut(s) 3808, 3843
CviQI GTAC 8 cut(s) 1047, 1089, 2213, 2348, 2557, 3395, 3459, 3749
DraI TTTAAA 1 cut(s) 3724
DraIII CACNNNGTG 1 cut(s) 1682
DriI GACNNNNNGTC 1 cut(s) 25
EaeI YGGCCR 6 cut(s) 170, 176, 1502, 2466, 3194, 3454
EagI CGGCCG 2 cut(s) 170, 176
Eam1104I CTCTTC 2 cut(s) 2808, 3319
Eam1105I GACNNNNNGTC 1 cut(s) 25
EarI CTCTTC 2 cut(s) 2808, 3319
EciI GGCGGA 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 813
EclXI CGGCCG 2 cut(s) 170, 176
Eco130I CCWWGG 4 cut(s) 888, 1524, 1575, 2718
Eco147I AGGCCT 1 cut(s) 2738
Eco24I GRGCYC 3 cut(s) 388, 815, 2202
Eco47I GGWCC 4 cut(s) 27, 134, 461, 1709
Eco47III AGCGCT 2 cut(s) 806, 3770
Eco52I CGGCCG 2 cut(s) 170, 176
Eco53kI GAGCTC 1 cut(s) 813
Eco57I CTGAAG 3 cut(s) 1281, 2337, 3831
Eco88I CYCGRG 1 cut(s) 3692
EcoICRI GAGCTC 1 cut(s) 813
EcoRI GAATTC 2 cut(s) 688, 1777
EcoRII CCWGG 1 cut(s) 1558
EcoT14I CCWWGG 4 cut(s) 888, 1524, 1575, 2718
EcoT38I GRGCYC 3 cut(s) 388, 815, 2202
ErhI CCWWGG 4 cut(s) 888, 1524, 1575, 2718
Esp3I CGTCTC 2 cut(s) 80, 236
FalI AAGNNNNNCTT 2 cut(s) 2431, 2463
FaqI GGGAC 5 cut(s) 13, 474, 1457, 2468, 3365
FauI CCCGC 3 cut(s) 211, 375, 1267
FauNDI CATATG 4 cut(s) 1591, 2920, 3742, 3955
FbaI TGATCA 3 cut(s) 499, 529, 1747
FblI GTMKAC 2 cut(s) 2660, 3834
FriOI GRGCYC 3 cut(s) 388, 815, 2202
FspI TGCGCA 1 cut(s) 2060
GlaI GCGC 4 cut(s) 358, 806, 2060, 3770
GsaI CCCAGC 1 cut(s) 252
GsuI CTGGAG 3 cut(s) 829, 1302, 1860
HaeII RGCGCY 3 cut(s) 360, 808, 3772
HapII CCGG 8 cut(s) 8, 25, 51, 236, 254, 458, 660, 2580
HgaI GACGC 5 cut(s) 29, 97, 2039, 3820, 3876
HhaI GCGC 4 cut(s) 359, 807, 2061, 3771
Hin1I GRCGYC 4 cut(s) 21, 89, 2050, 3831
Hin6I GCGC 4 cut(s) 357, 805, 2059, 3769
HinP1I GCGC 4 cut(s) 357, 805, 2059, 3769
HincII GTYRAC 2 cut(s) 262, 1810
HindII GTYRAC 2 cut(s) 262, 1810
HindIII AAGCTT 4 cut(s) 2286, 2445, 2678, 3240
HpaII CCGG 8 cut(s) 8, 25, 51, 236, 254, 458, 660, 2580
Hpy166II GTNNAC 9 cut(s) 262, 1122, 1810, 2661, 2834, 3089, 3461, 3700, 3835
Hpy8I GTNNAC 9 cut(s) 262, 1122, 1810, 2661, 2834, 3089, 3461, 3700, 3835
Hpy99I CGWCG 2 cut(s) 134, 3489
HpyCH4IV ACGT 5 cut(s) 1902, 2096, 3085, 3487, 3826
HpyF10VI GCNNNNNNNGC 8 cut(s) 20, 245, 1271, 1311, 2056, 2714, 3239, 3453
HpySE526I ACGT 5 cut(s) 1902, 2096, 3085, 3487, 3826
Hsp92I GRCGYC 4 cut(s) 21, 89, 2050, 3831
HspAI GCGC 4 cut(s) 357, 805, 2059, 3769
Kpn2I TCCGGA 1 cut(s) 2579
KpnI GGTACC 1 cut(s) 1092
KroI GCCGGC 2 cut(s) 7, 235
KroNI GCCGGC 2 cut(s) 9, 237
Ksp22I TGATCA 3 cut(s) 499, 529, 1747
KspI CCGCGG 1 cut(s) 176
LguI GCTCTTC 1 cut(s) 3319
LmnI GCTCC 8 cut(s) 550, 802, 810, 1691, 1841, 1868, 3154, 3452
MaeII ACGT 5 cut(s) 1902, 2096, 3085, 3487, 3826
MaeIII GTNAC 8 cut(s) 156, 491, 749, 1021, 1076, 2766, 3076, 3413
MbiI CCGCTC 1 cut(s) 382
MfeI CAATTG 2 cut(s) 1224, 1813
MflI RGATCY 3 cut(s) 3020, 3188, 3757
MhlI GDGCHC 3 cut(s) 388, 815, 2202
MlsI TGGCCA 2 cut(s) 1504, 2468
MluNI TGGCCA 2 cut(s) 1504, 2468
MlyI GAGTC 3 cut(s) 623, 3711, 3868
MmeI TCCRAC 5 cut(s) 153, 938, 1264, 3908, 3926
Mox20I TGGCCA 2 cut(s) 1504, 2468
MreI CGCCGGCG 1 cut(s) 7
MroI TCCGGA 1 cut(s) 2579
MroNI GCCGGC 2 cut(s) 7, 235
MroXI GAANNNNTTC 2 cut(s) 2341, 3844
MscI TGGCCA 2 cut(s) 1504, 2468
MslI CAYNNNNRTG 5 cut(s) 1421, 1561, 2919, 3105, 3705
Msp20I TGGCCA 2 cut(s) 1504, 2468
MspA1I CMGCKG 3 cut(s) 175, 858, 1199
MspCI CTTAAG 1 cut(s) 2378
MspI CCGG 8 cut(s) 8, 25, 51, 236, 254, 458, 660, 2580
MspR9I CCNGG 3 cut(s) 25, 459, 1560
MunI CAATTG 2 cut(s) 1224, 1813
Mva1269I GAATGC 1 cut(s) 220
MvaI CCWGG 1 cut(s) 1560
MvnI CGCG 1 cut(s) 175
MwoI GCNNNNNNNGC 8 cut(s) 20, 245, 1271, 1311, 2056, 2714, 3239, 3453
NaeI GCCGGC 2 cut(s) 9, 237
NciI CCSGG 2 cut(s) 25, 459
NcoI CCATGG 1 cut(s) 1575
NdeI CATATG 4 cut(s) 1591, 2920, 3742, 3955
NgoMIV GCCGGC 2 cut(s) 7, 235
NheI GCTAGC 1 cut(s) 3368
NlaIV GGNNCC 7 cut(s) 29, 127, 462, 658, 871, 1090, 1687
NmeAIII GCCGAG 4 cut(s) 148, 204, 2511, 3172
NmuCI GTSAC 5 cut(s) 156, 491, 749, 3076, 3413
NsbI TGCGCA 1 cut(s) 2060
NspV TTCGAA 1 cut(s) 786
PaeR7I CTCGAG 1 cut(s) 3692
PagI TCATGA 2 cut(s) 826, 2248
PceI AGGCCT 1 cut(s) 2738
PciSI GCTCTTC 1 cut(s) 3319
PctI GAATGC 1 cut(s) 220
PdiI GCCGGC 2 cut(s) 9, 237
PdmI GAANNNNTTC 2 cut(s) 2341, 3844
PflMI CCANNNNNTGG 1 cut(s) 3244
PfoI TCCNGGA 1 cut(s) 457
PleI GAGTC 3 cut(s) 623, 3711, 3867
PpsI GAGTC 3 cut(s) 623, 3711, 3867
Ppu21I YACGTR 1 cut(s) 3086
PshAI GACNNNNGTC 1 cut(s) 3276
PshBI ATTAAT 1 cut(s) 1182
PsiI TTATAA 1 cut(s) 2105
Psp124BI GAGCTC 1 cut(s) 815
Psp6I CCWGG 1 cut(s) 1558
PspFI CCCAGC 1 cut(s) 248
PspGI CCWGG 1 cut(s) 1558
PspN4I GGNNCC 7 cut(s) 29, 127, 462, 658, 871, 1090, 1687
PspPI GGNCC 7 cut(s) 27, 134, 233, 251, 461, 1205, 1709
PsrI GAACNNNNNNTAC 2 cut(s) 853, 885
PstI CTGCAG 3 cut(s) 2026, 3232, 3278
PstNI CAGNNNCTG 2 cut(s) 1202, 1271
PsuI RGATCY 3 cut(s) 3020, 3188, 3757
PvuII CAGCTG 1 cut(s) 1199
RsaI GTAC 8 cut(s) 1048, 1090, 2214, 2349, 2558, 3396, 3460, 3750
RsaNI GTAC 8 cut(s) 1047, 1089, 2213, 2348, 2557, 3395, 3459, 3749
RseI CAYNNNNRTG 5 cut(s) 1421, 1561, 2919, 3105, 3705
SacI GAGCTC 1 cut(s) 815
SacII CCGCGG 1 cut(s) 176
SapI GCTCTTC 1 cut(s) 3319
Sau96I GGNCC 7 cut(s) 27, 134, 233, 251, 461, 1205, 1709
ScaI AGTACT 2 cut(s) 1048, 3750
SchI GAGTC 3 cut(s) 623, 3711, 3868
ScrFI CCNGG 3 cut(s) 25, 459, 1560
SduI GDGCHC 3 cut(s) 388, 815, 2202
SfcI CTRYAG 9 cut(s) 1602, 1635, 2022, 2398, 2697, 3228, 3274, 3835, 3877
Sfr274I CTCGAG 1 cut(s) 3692
Sfr303I CCGCGG 1 cut(s) 176
SfuI TTCGAA 1 cut(s) 786
SgrAI CRCCGGYG 1 cut(s) 7
SgrBI CCGCGG 1 cut(s) 176
SinI GGWCC 4 cut(s) 27, 134, 461, 1709
SlaI CTCGAG 1 cut(s) 3692
SmiMI CAYNNNNRTG 5 cut(s) 1421, 1561, 2919, 3105, 3705
SmlI CTYRAG 5 cut(s) 2378, 2999, 3608, 3692, 3785
SmoI CTYRAG 5 cut(s) 2378, 2999, 3608, 3692, 3785
SpeI ACTAGT 2 cut(s) 302, 1013
SseBI AGGCCT 1 cut(s) 2738
SspI AATATT 3 cut(s) 599, 1803, 3184
SstI GAGCTC 1 cut(s) 815
StuI AGGCCT 1 cut(s) 2738
StyD4I CCNGG 3 cut(s) 23, 457, 1558
StyI CCWWGG 4 cut(s) 888, 1524, 1575, 2718
TaiI ACGT 5 cut(s) 1905, 2099, 3088, 3490, 3829
TaqI TCGA 7 cut(s) 224, 522, 786, 1622, 3484, 3693, 3909
TaqII GACCGA 1 cut(s) 478
TatI WGTACW 3 cut(s) 1046, 3394, 3748
TauI GCSGC 5 cut(s) 14, 17, 175, 178, 1739
TscAI CASTG 9 cut(s) 502, 1102, 1120, 1644, 1774, 2476, 2719, 2884, 3156
TseFI GTSAC 5 cut(s) 156, 491, 749, 3076, 3413
Tsp45I GTSAC 5 cut(s) 156, 491, 749, 3076, 3413
TspGWI ACGGA 2 cut(s) 604, 3487
TspRI CASTG 9 cut(s) 502, 1102, 1120, 1644, 1774, 2476, 2719, 2884, 3156
Van91I CCANNNNNTGG 1 cut(s) 3244
Vha464I CTTAAG 1 cut(s) 2378
VpaK11BI GGWCC 4 cut(s) 27, 134, 461, 1709
VspI ATTAAT 1 cut(s) 1182
XapI RAATTY 8 cut(s) 550, 688, 788, 1730, 1777, 1924, 2515, 3532
XbaI TCTAGA 1 cut(s) 3320
XcmI CCANNNNNNNNNTGG 1 cut(s) 1061
XhoI CTCGAG 1 cut(s) 3692
XmiI GTMKAC 2 cut(s) 2660, 3834
XmnI GAANNNNTTC 2 cut(s) 2341, 3844
ZrmI AGTACT 2 cut(s) 1048, 3750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.