Rorug04G0032800

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0016819
Physical Location & Seq
Reverse (-)
4846531 .. 4848597
2067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0032800.1

Sequence Viewer

Length: 1491 bp
ATGTCTGCATATCGTAAAAGTGGTAGAGATCATACAGTTATTAATATTATCGATGACCGCGAGAAAAGCAAGAAATGCAAGAAATGCATCTTCAGAGTTCCTAACGTACTCCGGAGACAAAACCCAGAAGCATATACACCTGACGTTGTTTCAATCGGACCTTTTCGTCATTATCGAGAAAAGCGAGGCAAGGAGGAAGGTAAAGGAGTCAAGGAAGGCAAACAAGGCATAGGCGAGGAAGATTTCCAACTTACGAAACGAGTGAAAAAGAGCTATTTGAATGAAATTCTCACAGGTATGAAGATAACTTTGGAAGAGTTGACCGCAAAAGTTACTGAGCTCTCAGATCAGAAGAATGAAGGTGGCTTTGAGGAACGAGCCCGCAATTTTTATGCAGAACCATTTGATCATATTTCTTCCAAAGACTTCATCGAGATGATGATAGTTGACGGTTGCTTCCTAATTCAACTGTTTCGGAAGTGTAACTATCCAAAACTCAGGGCCTTTGATGATCCGGTGTTCAACATGGACTGTATATTTCATTTCCTATGCCATGACATTTTGCTTCTAGAGAATCAACTACCTTGGTTTGTTATCCACTGTTTGTATAGCCTTACCCTTGAAATATACCCTGATGAAGCCTCCCTCTCTACTCTCATCCTTAAAGCCTTCAGCATATTACCATCACTGAAGCAAAGTTGCTCATCTTATAATAAGCATCTCCGCCAAAGCAAGTGTCATTGTGATGCTGATTATCTGCACATACTTGATCTGGTAAGAGCTTCCATAGTTATTCCATTAAAGACCATCGATGTGAGAGTAGCTGGAAAAGAATCTATGTCCAGCCAAGCTGAAGAGCATCAAGTGCATAAAAGGACCATTGACAAGGCAGAGGGTAAAGCTGTTCAGGAAACTATCTTTGACCCAGATCTACATCATATATGTACCGCAACCGCTCTCTCAAAGGCAGACATTAAATTCAAAAGCGTAGAAAAGGAGAGCATAATGGACATCCAATTCAAAGAGGGTGGGTGGTTCAGGAATGGAATTCTTATGATTCCACAGCTAAAGGTTGGAATGTCATCAGAAACATTATTCAGGAACCTCATTGCAATTGAGCAATGCTACCATGGTTATTCAAACGAGATAACATCTTATGCCATCTTTATGGATAACCTCATCTCTTCAAAGGAAGATATGGAATTACTTTGCAAGGAAAAAGTAATTGGTAACTCGATGAGTGATGAAGATGGTTGTAAGTTTTTCAGCAACCTTTACAAAGACATCCCGCACAACAAGTTCTGCTATGTTGACCTGTGCGAAAAAGTGAATGATCGTTACAGATCGAGATGGTTTACATGGTTGGCTTTACTCAAGAGTGAAAAATTTTCTAGTCCCTGGAGAATTTTAGCTTTTGGGATAGGTATTATCATTTTGATTCTCACCGCGTGGAGTCAGACACTCAACATTCGGGCTAACATGCATAAGTGA

Protein Analysis

496

Amino Acids

57.37

Weight (kDa)

8.04

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 30 - 481 1.4e-93 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016819)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08080
malus_domestica MD13G1252600.v1.1 MD16G1256800.v1.1
prunus_persica Prupe.1G084800_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0401791
rosa_laevigata RLG00000009120
rosa_multiflora Rmu_sc0001405.1_g000029
rosa_roxburghii Rroxscaffold_5G00346350
rosa_rugosa Rorug04G0032800
rosa_samantha Rh4AG109200 Rh4BG103400 Rh4CG117400 Rh4DG101200
rosa_wichuraiana Rw4G008890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 711
AasI GACNNNNNNGTC 1 cut(s) 165
AccBSI CCGCTC 1 cut(s) 956
AccII CGCG 2 cut(s) 60, 1448
AccIII TCCGGA 1 cut(s) 111
AciI CCGC 8 cut(s) 58, 324, 382, 724, 948, 954, 1289, 1446
AclWI GGATC 1 cut(s) 506
AcsI RAATTY 5 cut(s) 285, 977, 1047, 1385, 1404
AcuI CTGAAG 4 cut(s) 76, 655, 710, 873
AdeI CACNNNGTG 1 cut(s) 1449
AfaI GTAC 2 cut(s) 108, 946
AgsI TTSAA 9 cut(s) 153, 280, 467, 523, 623, 982, 1021, 1140, 1188
AjnI CCWGG 1 cut(s) 1397
AjuI GAANNNNNNNTTGG 4 cut(s) 293, 325, 1209, 1241
AluBI AGCT 8 cut(s) 273, 340, 782, 824, 851, 902, 1066, 1412
AluI AGCT 8 cut(s) 273, 340, 782, 824, 851, 902, 1066, 1412
Alw21I GWGCWC 1 cut(s) 342
Alw26I GTCTC 1 cut(s) 109
AlwI GGATC 1 cut(s) 506
Aor13HI TCCGGA 1 cut(s) 111
AoxI GGCC 1 cut(s) 501
ApoI RAATTY 5 cut(s) 285, 977, 1047, 1385, 1404
ArsI GACNNNNNNTTYG 2 cut(s) 721, 753
AseI ATTAAT 1 cut(s) 42
AspS9I GGNCC 3 cut(s) 158, 501, 876
AsuHPI GGTGA 1 cut(s) 1435
AvaII GGWCC 2 cut(s) 158, 876
BanII GRGCYC 2 cut(s) 342, 382
Bbv12I GWGCWC 1 cut(s) 342
BccI CCATC 5 cut(s) 691, 815, 1169, 1244, 1344
BciT130I CCWGG 1 cut(s) 1399
BclI TGATCA 1 cut(s) 406
BcoDI GTCTC 1 cut(s) 109
BfaI CTAG 2 cut(s) 569, 1392
BglII AGATCT 1 cut(s) 928
Bme1390I CCNGG 1 cut(s) 1399
Bme18I GGWCC 2 cut(s) 158, 876
BmgT120I GGNCC 3 cut(s) 158, 501, 876
BmiI GGNNCC 1 cut(s) 1103
BmrFI CCNGG 1 cut(s) 1399
BmsI GCATC 4 cut(s) 96, 727, 736, 868
BpmI CTGGAG 1 cut(s) 1420
BpuEI CTTGAG 1 cut(s) 1358
Bsa29I ATCGAT 2 cut(s) 51, 810
BsaBI GATNNNNATC 1 cut(s) 933
BsaJI CCNNGG 3 cut(s) 584, 1129, 1397
BsaWI WCCGGW 2 cut(s) 111, 514
Bse3DI GCAATG 2 cut(s) 1107, 1127
Bse8I GATNNNNATC 1 cut(s) 933
BseAI TCCGGA 1 cut(s) 111
BseBI CCWGG 1 cut(s) 1399
BseCI ATCGAT 2 cut(s) 51, 810
BseDI CCNNGG 3 cut(s) 584, 1129, 1397
BseGI GGATG 3 cut(s) 657, 1011, 1284
BseJI GATNNNNATC 1 cut(s) 933
BseMI GCAATG 2 cut(s) 1107, 1127
BseMII CTCAG 3 cut(s) 327, 357, 511
BsgI GTGCAG 1 cut(s) 743
Bsh1236I CGCG 2 cut(s) 60, 1448
BshFI GGCC 1 cut(s) 503
BshVI ATCGAT 2 cut(s) 51, 810
BsiHKAI GWGCWC 1 cut(s) 342
BsiSI CCGG 2 cut(s) 112, 515
BslFI GGGAC 1 cut(s) 1380
BsmAI GTCTC 1 cut(s) 109
BsmFI GGGAC 1 cut(s) 1380
BsnI GGCC 1 cut(s) 503
Bsp1286I GDGCHC 2 cut(s) 342, 382
Bsp13I TCCGGA 1 cut(s) 111
Bsp143I GATC 8 cut(s) 28, 346, 406, 511, 769, 928, 1333, 1343
Bsp19I CCATGG 1 cut(s) 1129
BspACI CCGC 8 cut(s) 58, 324, 382, 724, 948, 954, 1289, 1446
BspANI GGCC 1 cut(s) 503
BspCNI CTCAG 3 cut(s) 328, 356, 510
BspDI ATCGAT 2 cut(s) 51, 810
BspEI TCCGGA 1 cut(s) 111
BspFNI CGCG 2 cut(s) 60, 1448
BspLI GGNNCC 1 cut(s) 1103
BspPI GGATC 1 cut(s) 506
BspQI GCTCTTC 1 cut(s) 849
BsrBI CCGCTC 1 cut(s) 956
BsrDI GCAATG 2 cut(s) 1107, 1127
BssECI CCNNGG 3 cut(s) 584, 1129, 1397
BssMI GATC 8 cut(s) 28, 346, 406, 511, 769, 928, 1333, 1343
BssT1I CCWWGG 2 cut(s) 584, 1129
Bst2UI CCWGG 1 cut(s) 1399
Bst4CI ACNGT 5 cut(s) 37, 452, 471, 533, 602
Bst6I CTCTTC 3 cut(s) 309, 849, 1189
BstAPI GCANNNNNTGC 3 cut(s) 75, 84, 865
BstC8I GCNNGC 1 cut(s) 382
BstDEI CTNAG 3 cut(s) 336, 343, 497
BstDSI CCRYGG 1 cut(s) 1129
BstF5I GGATG 3 cut(s) 657, 1011, 1284
BstFNI CGCG 2 cut(s) 60, 1448
BstKTI GATC 8 cut(s) 31, 349, 409, 514, 772, 931, 1336, 1346
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 8 cut(s) 28, 346, 406, 511, 769, 928, 1333, 1343
BstMWI GCNNNNNNNGC 5 cut(s) 66, 75, 84, 225, 865
BstNI CCWGG 1 cut(s) 1399
BstNSI RCATGY 1 cut(s) 1483
BstSCI CCNGG 1 cut(s) 1397
BstUI CGCG 2 cut(s) 60, 1448
BstX2I RGATCY 1 cut(s) 928
BstXI CCANNNNNNTGG 1 cut(s) 1168
BstYI RGATCY 1 cut(s) 928
Bsu15I ATCGAT 2 cut(s) 51, 810
BsuRI GGCC 1 cut(s) 503
BsuTUI ATCGAT 2 cut(s) 51, 810
BtgI CCRYGG 1 cut(s) 1129
BtsCI GGATG 3 cut(s) 657, 1011, 1284
BtsIMutI CAGTG 2 cut(s) 598, 686
Cac8I GCNNGC 1 cut(s) 382
Cfr13I GGNCC 3 cut(s) 158, 501, 876
ClaI ATCGAT 2 cut(s) 51, 810
Csp6I GTAC 2 cut(s) 107, 945
CspCI CAANNNNNGTGG 2 cut(s) 1009, 1044
CviAII CATG 5 cut(s) 526, 554, 1130, 1359, 1480
CviQI GTAC 2 cut(s) 107, 945
DdeI CTNAG 3 cut(s) 336, 343, 497
DpnI GATC 8 cut(s) 30, 348, 408, 513, 771, 930, 1335, 1345
DpnII GATC 8 cut(s) 28, 346, 406, 511, 769, 928, 1333, 1343
DraIII CACNNNGTG 1 cut(s) 1449
DrdI GACNNNNNNGTC 1 cut(s) 165
DseDI GACNNNNNNGTC 1 cut(s) 165
Eam1104I CTCTTC 3 cut(s) 309, 849, 1189
EarI CTCTTC 3 cut(s) 309, 849, 1189
EciI GGCGGA 1 cut(s) 713
Ecl136II GAGCTC 1 cut(s) 340
Eco130I CCWWGG 2 cut(s) 584, 1129
Eco24I GRGCYC 2 cut(s) 342, 382
Eco47I GGWCC 2 cut(s) 158, 876
Eco53kI GAGCTC 1 cut(s) 340
Eco57I CTGAAG 4 cut(s) 76, 655, 710, 873
EcoICRI GAGCTC 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 501
EcoRI GAATTC 1 cut(s) 1047
EcoRII CCWGG 1 cut(s) 1397
EcoT14I CCWWGG 2 cut(s) 584, 1129
EcoT22I ATGCAT 2 cut(s) 89, 1485
EcoT38I GRGCYC 2 cut(s) 342, 382
ErhI CCWWGG 2 cut(s) 584, 1129
FaeI CATG 5 cut(s) 529, 557, 1133, 1362, 1483
FaqI GGGAC 1 cut(s) 1380
FatI CATG 5 cut(s) 525, 553, 1129, 1358, 1479
FauI CCCGC 2 cut(s) 389, 1296
FbaI TGATCA 1 cut(s) 406
FokI GGATG 3 cut(s) 644, 998, 1271
FriOI GRGCYC 2 cut(s) 342, 382
FspBI CTAG 2 cut(s) 569, 1392
GsuI CTGGAG 1 cut(s) 1420
HaeIII GGCC 1 cut(s) 503
HapII CCGG 2 cut(s) 112, 515
Hin1II CATG 5 cut(s) 529, 557, 1133, 1362, 1483
HincII GTYRAC 3 cut(s) 321, 448, 1312
HindII GTYRAC 3 cut(s) 321, 448, 1312
HinfI GANTC 6 cut(s) 207, 574, 833, 1057, 1438, 1453
HpaII CCGG 2 cut(s) 112, 515
HphI GGTGA 1 cut(s) 1435
Hpy166II GTNNAC 4 cut(s) 321, 448, 1312, 1356
Hpy188I TCNGA 7 cut(s) 95, 158, 346, 351, 477, 1087, 1458
Hpy188III TCNNGA 9 cut(s) 112, 176, 433, 569, 908, 1039, 1099, 1347, 1375
Hpy8I GTNNAC 4 cut(s) 321, 448, 1312, 1356
HpyAV CCTTC 4 cut(s) 191, 209, 353, 679
HpyCH4III ACNGT 5 cut(s) 37, 452, 471, 533, 602
HpyCH4IV ACGT 2 cut(s) 105, 144
HpyCH4V TGCA 9 cut(s) 8, 78, 87, 395, 760, 868, 1112, 1212, 1483
HpyF10VI GCNNNNNNNGC 5 cut(s) 66, 75, 84, 225, 865
HpyF3I CTNAG 3 cut(s) 336, 343, 497
HpySE526I ACGT 2 cut(s) 105, 144
Hsp92II CATG 5 cut(s) 529, 557, 1133, 1362, 1483
Kpn2I TCCGGA 1 cut(s) 111
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 8 cut(s) 28, 346, 406, 511, 769, 928, 1333, 1343
LguI GCTCTTC 1 cut(s) 849
LweI GCATC 4 cut(s) 96, 727, 736, 868
MaeI CTAG 2 cut(s) 569, 1392
MaeII ACGT 2 cut(s) 105, 144
MaeIII GTNAC 4 cut(s) 331, 482, 1229, 1337
MalI GATC 8 cut(s) 30, 348, 408, 513, 771, 930, 1335, 1345
MbiI CCGCTC 1 cut(s) 956
MboI GATC 8 cut(s) 28, 346, 406, 511, 769, 928, 1333, 1343
MfeI CAATTG 1 cut(s) 1113
MflI RGATCY 1 cut(s) 928
MhlI GDGCHC 2 cut(s) 342, 382
MlyI GAGTC 2 cut(s) 216, 1462
MmeI TCCRAC 2 cut(s) 271, 1054
Mph1103I ATGCAT 2 cut(s) 89, 1485
MroI TCCGGA 1 cut(s) 111
MseI TTAA 4 cut(s) 42, 663, 800, 975
MslI CAYNNNNRTG 5 cut(s) 296, 434, 744, 812, 1166
MspI CCGG 2 cut(s) 112, 515
MspR9I CCNGG 1 cut(s) 1399
MunI CAATTG 1 cut(s) 1113
MvaI CCWGG 1 cut(s) 1399
MvnI CGCG 2 cut(s) 60, 1448
MwoI GCNNNNNNNGC 5 cut(s) 66, 75, 84, 225, 865
NcoI CCATGG 1 cut(s) 1129
NdeII GATC 8 cut(s) 28, 346, 406, 511, 769, 928, 1333, 1343
NlaIII CATG 5 cut(s) 529, 557, 1133, 1362, 1483
NlaIV GGNNCC 1 cut(s) 1103
NsiI ATGCAT 2 cut(s) 89, 1485
NspI RCATGY 1 cut(s) 1483
PciSI GCTCTTC 1 cut(s) 849
PcsI WCGNNNNNNNCGW 3 cut(s) 57, 172, 181
PfeI GAWTC 4 cut(s) 574, 833, 1057, 1438
PleI GAGTC 2 cut(s) 215, 1461
PpsI GAGTC 2 cut(s) 215, 1461
PshBI ATTAAT 1 cut(s) 42
PsiI TTATAA 1 cut(s) 711
Psp124BI GAGCTC 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 1397
PspGI CCWGG 1 cut(s) 1397
PspN4I GGNNCC 1 cut(s) 1103
PspPI GGNCC 3 cut(s) 158, 501, 876
PsuI RGATCY 1 cut(s) 928
RsaI GTAC 2 cut(s) 108, 946
RsaNI GTAC 2 cut(s) 107, 945
RseI CAYNNNNRTG 5 cut(s) 296, 434, 744, 812, 1166
SacI GAGCTC 1 cut(s) 342
SapI GCTCTTC 1 cut(s) 849
SaqAI TTAA 4 cut(s) 42, 663, 800, 975
Sau3AI GATC 8 cut(s) 28, 346, 406, 511, 769, 928, 1333, 1343
Sau96I GGNCC 3 cut(s) 158, 501, 876
SchI GAGTC 2 cut(s) 216, 1462
ScrFI CCNGG 1 cut(s) 1399
SduI GDGCHC 2 cut(s) 342, 382
SfaNI GCATC 4 cut(s) 96, 727, 736, 868
SinI GGWCC 2 cut(s) 158, 876
SmiMI CAYNNNNRTG 5 cut(s) 296, 434, 744, 812, 1166
SmlI CTYRAG 1 cut(s) 1373
SmoI CTYRAG 1 cut(s) 1373
SsiI CCGC 8 cut(s) 58, 324, 382, 724, 948, 954, 1289, 1446
SspI AATATT 1 cut(s) 46
SspMI CTAG 2 cut(s) 569, 1392
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 1 cut(s) 1397
StyI CCWWGG 2 cut(s) 584, 1129
TaaI ACNGT 5 cut(s) 37, 452, 471, 533, 602
TaiI ACGT 2 cut(s) 108, 147
TaqI TCGA 6 cut(s) 51, 175, 432, 810, 1235, 1346
TfiI GAWTC 4 cut(s) 574, 833, 1057, 1438
Tru1I TTAA 4 cut(s) 42, 663, 800, 975
Tru9I TTAA 4 cut(s) 42, 663, 800, 975
TscAI CASTG 2 cut(s) 605, 693
TspDTI ATGAA 7 cut(s) 297, 314, 372, 418, 530, 651, 1260
TspRI CASTG 2 cut(s) 605, 693
VpaK11BI GGWCC 2 cut(s) 158, 876
VspI ATTAAT 1 cut(s) 42
XapI RAATTY 5 cut(s) 285, 977, 1047, 1385, 1404
XbaI TCTAGA 1 cut(s) 568
XceI RCATGY 1 cut(s) 1483
XspI CTAG 2 cut(s) 569, 1392
Zsp2I ATGCAT 2 cut(s) 89, 1485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.