Rorug04G0069800

metalloendopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0015526
Physical Location & Seq
Reverse (-)
10767014 .. 10768214
1201 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0069800.1

Sequence Viewer

Length: 978 bp
ATGGCAGCAACTGATCATATCCCAGAGGTGGTTCTTGAATCCTCCAATGGCCGCAGGACCATGCCTGTGCTTGGCTTCGGCACAGCATCCAACAACTTACAACCAGAGGTTTTCATAGAAGCTGTTCTCGAGGCCATCAAGCTCGGTTACCGCCACTTCGACACAGCTTCCCTGTACGGCTCGGAGCAGAGTCTGGGAGTAGCCATAGCCCAAGCACTCAAACTCGGCCTCGTGGCTTCTCGAGACGAGCTCTTCATCACTTCCAAGCTTTGGTCTAATGATGCTCACCCTGATTTGGTTCTTCCTGCTCTAAAGAAATCCCTTCAGAATCTTCAATTGGATTACCTTGATCTGTATCTGATTCACTGGCCCATCAGTGCGACGCCTGGGAAATTGAGTTACTTTCTCGAGGAGAAGGACCAAATGTCGATGGACTTCAAGGGCGTGTGGGCAGCCATGGAAGAAGCTCAGAGACTTGGCCTCACCAAATCCATTGGAATCAGCAATTTCTCTACCAAAAAGACTCAGAATTTGCTCTCTTTTGCTACTATTCCTCCTTCAGTCAATCAAGTTGAGATGAGCCCATTTTGGCAACAAAAGAAGCTCAGAGAATTCTGTAAAGCCAATGGTATAGCTGTGACTGCCTTCTCCCCATTGGGTGCCATAGGAACCAGTTGGGGTACCAATTATGTTCTCGAAAGCAAAGTGCTTAATGAAATCGCTGAGGCTCGGGGAAAGACTGTTGCTCAGGTCTGCATTAGATGGGTTTATCAGGTAGGAGCAACTCTTGCAGTGAAGAGCTACAACAAGGAGAGGTTGAAGCAGAATGTGCAGGTGTTCGACTGGGAACTAACAGAAGAGGATCTTGAGAAGATCAATCAAATCCCACAGCGCAGAATGATATCTCGAGAAGAAGAGTTGGTTACGGCTACTGGACCATACAAGTCCCTTGATGAGTTATGGGACGGAGATTATTAA

Protein Analysis

325

Amino Acids

36.43

Weight (kDa)

5.43

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 24 - 295 1.5e-57 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 823
Acc36I ACCTGC 1 cut(s) 823
Acc65I GGTACC 1 cut(s) 680
AccB1I GGYRCC 2 cut(s) 659, 680
AccB7I CCANNNNNTGG 1 cut(s) 270
AciI CCGC 2 cut(s) 52, 151
AclWI GGATC 1 cut(s) 870
AcoI YGGCCR 1 cut(s) 49
AcsI RAATTY 2 cut(s) 529, 611
AcuI CTGAAG 2 cut(s) 308, 543
AcyI GRCGYC 1 cut(s) 383
AfaI GTAC 2 cut(s) 176, 682
AfiI CCNNNNNNNGG 4 cut(s) 28, 71, 270, 295
AgsI TTSAA 4 cut(s) 38, 335, 439, 820
AhdI GACNNNNNGTC 1 cut(s) 424
AjnI CCWGG 1 cut(s) 385
AjuI GAANNNNNNNTTGG 2 cut(s) 320, 352
AluBI AGCT 9 cut(s) 122, 142, 167, 250, 268, 467, 604, 635, 801
AluI AGCT 9 cut(s) 122, 142, 167, 250, 268, 467, 604, 635, 801
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 2 cut(s) 237, 466
AlwI GGATC 1 cut(s) 870
AlwNI CAGNNNCTG 2 cut(s) 11, 193
Ama87I CYCGRG 5 cut(s) 128, 240, 407, 729, 906
AoxI GGCC 5 cut(s) 49, 132, 226, 368, 478
ApeKI GCWGC 2 cut(s) 5, 452
ApoI RAATTY 2 cut(s) 529, 611
ArsI GACNNNNNNTTYG 2 cut(s) 514, 546
Asp700I GAANNNNTTC 1 cut(s) 123
Asp718I GGTACC 1 cut(s) 680
AspLEI GCGC 1 cut(s) 894
AspS9I GGNCC 4 cut(s) 57, 369, 418, 935
AsuHPI GGTGA 2 cut(s) 278, 475
AvaI CYCGRG 5 cut(s) 128, 240, 407, 729, 906
AvaII GGWCC 3 cut(s) 57, 418, 935
BanI GGYRCC 2 cut(s) 659, 680
BanII GRGCYC 2 cut(s) 252, 584
BauI CACGAG 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 252
BbvCI CCTCAGC 1 cut(s) 723
BbvI GCAGC 2 cut(s) 17, 464
BccI CCATC 4 cut(s) 143, 380, 424, 756
BceAI ACGGC 2 cut(s) 193, 942
BciT130I CCWGG 1 cut(s) 387
BclI TGATCA 1 cut(s) 13
BcoDI GTCTC 2 cut(s) 237, 466
BfuAI ACCTGC 1 cut(s) 823
BisI GCNGC 3 cut(s) 6, 52, 453
BlsI GCNGC 3 cut(s) 7, 53, 454
Bme1390I CCNGG 1 cut(s) 387
Bme18I GGWCC 3 cut(s) 57, 418, 935
BmeRI GACNNNNNGTC 1 cut(s) 424
BmeT110I CYCGRG 5 cut(s) 128, 240, 407, 729, 906
BmgT120I GGNCC 4 cut(s) 57, 369, 418, 935
BmiI GGNNCC 3 cut(s) 661, 670, 682
BmrFI CCNGG 1 cut(s) 387
BmrI ACTGGG 1 cut(s) 853
BmsI GCATC 2 cut(s) 95, 271
BmuI ACTGGG 1 cut(s) 853
BplI GAGNNNNNCTC 2 cut(s) 234, 266
Bpu10I CCTNAGC 2 cut(s) 723, 747
BpuEI CTTGAG 1 cut(s) 887
BsaBI GATNNNNATC 1 cut(s) 354
BsaHI GRCGYC 1 cut(s) 383
BsaJI CCNNGG 2 cut(s) 386, 456
BsaXI ACNNNNNCTCC 2 cut(s) 538, 568
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 71, 270, 295
Bse1I ACTGG 4 cut(s) 371, 672, 848, 937
Bse8I GATNNNNATC 1 cut(s) 354
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 2 cut(s) 386, 456
BseGI GGATG 1 cut(s) 86
BseJI GATNNNNATC 1 cut(s) 354
BseLI CCNNNNNNNGG 4 cut(s) 28, 71, 270, 295
BseMII CTCAG 5 cut(s) 482, 539, 619, 714, 761
BseNI ACTGG 4 cut(s) 371, 672, 848, 937
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 2 cut(s) 17, 464
BsgI GTGCAG 1 cut(s) 851
BshFI GGCC 5 cut(s) 51, 134, 228, 370, 480
BshNI GGYRCC 2 cut(s) 659, 680
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 5 cut(s) 128, 240, 407, 729, 906
BslFI GGGAC 1 cut(s) 931
BslI CCNNNNNNNGG 4 cut(s) 28, 71, 270, 295
BsmAI GTCTC 2 cut(s) 237, 466
BsmBI CGTCTC 1 cut(s) 237
BsmFI GGGAC 1 cut(s) 931
BsnI GGCC 5 cut(s) 51, 134, 228, 370, 480
BsoBI CYCGRG 5 cut(s) 128, 240, 407, 729, 906
Bsp1286I GDGCHC 2 cut(s) 252, 584
Bsp143I GATC 4 cut(s) 13, 349, 862, 873
Bsp19I CCATGG 1 cut(s) 456
BspACI CCGC 2 cut(s) 52, 151
BspANI GGCC 5 cut(s) 51, 134, 228, 370, 480
BspCNI CTCAG 5 cut(s) 481, 538, 618, 715, 760
BspLI GGNNCC 3 cut(s) 661, 670, 682
BspMI ACCTGC 1 cut(s) 823
BspPI GGATC 1 cut(s) 870
BspQI GCTCTTC 2 cut(s) 257, 791
BspT107I GGYRCC 2 cut(s) 659, 680
BsrI ACTGG 4 cut(s) 371, 672, 848, 937
BssECI CCNNGG 2 cut(s) 386, 456
BssMI GATC 4 cut(s) 13, 349, 862, 873
BssNI GRCGYC 1 cut(s) 383
BssSI CACGAG 1 cut(s) 230
BssT1I CCWWGG 1 cut(s) 456
Bst2BI CACGAG 1 cut(s) 230
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 742
Bst6I CTCTTC 4 cut(s) 257, 791, 852, 909
BstACI GRCGYC 1 cut(s) 383
BstAPI GCANNNNNTGC 2 cut(s) 788, 829
BstDEI CTNAG 5 cut(s) 468, 525, 605, 723, 747
BstDSI CCRYGG 1 cut(s) 456
BstEII GGTNACC 1 cut(s) 146
BstF5I GGATG 1 cut(s) 86
BstHHI GCGC 1 cut(s) 894
BstKTI GATC 4 cut(s) 16, 352, 865, 876
BstMAI GTCTC 2 cut(s) 237, 466
BstMBI GATC 4 cut(s) 13, 349, 862, 873
BstMWI GCNNNNNNNGC 3 cut(s) 641, 788, 829
BstNI CCWGG 1 cut(s) 387
BstPI GGTNACC 1 cut(s) 146
BstSCI CCNGG 1 cut(s) 385
BstV1I GCAGC 2 cut(s) 17, 464
BstX2I RGATCY 1 cut(s) 862
BstYI RGATCY 1 cut(s) 862
BsuRI GGCC 5 cut(s) 51, 134, 228, 370, 480
BtgI CCRYGG 1 cut(s) 456
BtsCI GGATG 1 cut(s) 86
BtsI GCAGTG 1 cut(s) 798
BtsIMutI CAGTG 3 cut(s) 364, 382, 798
BveI ACCTGC 1 cut(s) 823
CaiI CAGNNNCTG 2 cut(s) 11, 193
CfoI GCGC 1 cut(s) 894
Cfr13I GGNCC 4 cut(s) 57, 369, 418, 935
CseI GACGC 1 cut(s) 391
Csp6I GTAC 2 cut(s) 175, 681
CviAII CATG 2 cut(s) 61, 457
CviQI GTAC 2 cut(s) 175, 681
DdeI CTNAG 5 cut(s) 468, 525, 605, 723, 747
DpnI GATC 4 cut(s) 15, 351, 864, 875
DpnII GATC 4 cut(s) 13, 349, 862, 873
DriI GACNNNNNGTC 1 cut(s) 424
EaeI YGGCCR 1 cut(s) 49
Eam1104I CTCTTC 4 cut(s) 257, 791, 852, 909
Eam1105I GACNNNNNGTC 1 cut(s) 424
EarI CTCTTC 4 cut(s) 257, 791, 852, 909
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 456
Eco24I GRGCYC 2 cut(s) 252, 584
Eco32I GATATC 1 cut(s) 903
Eco47I GGWCC 3 cut(s) 57, 418, 935
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 2 cut(s) 308, 543
Eco88I CYCGRG 5 cut(s) 128, 240, 407, 729, 906
Eco91I GGTNACC 1 cut(s) 146
EcoICRI GAGCTC 1 cut(s) 250
EcoO65I GGTNACC 1 cut(s) 146
EcoRI GAATTC 1 cut(s) 611
EcoRII CCWGG 1 cut(s) 385
EcoRV GATATC 1 cut(s) 903
EcoT14I CCWWGG 1 cut(s) 456
EcoT38I GRGCYC 2 cut(s) 252, 584
ErhI CCWWGG 1 cut(s) 456
Esp3I CGTCTC 1 cut(s) 237
FaeI CATG 2 cut(s) 64, 460
FalI AAGNNNNNCTT 2 cut(s) 849, 881
FaqI GGGAC 1 cut(s) 931
FatI CATG 2 cut(s) 60, 456
FbaI TGATCA 1 cut(s) 13
Fnu4HI GCNGC 3 cut(s) 6, 52, 453
FokI GGATG 1 cut(s) 73
FriOI GRGCYC 2 cut(s) 252, 584
Fsp4HI GCNGC 3 cut(s) 6, 52, 453
GlaI GCGC 1 cut(s) 893
GluI GCNGC 3 cut(s) 6, 52, 453
HaeIII GGCC 5 cut(s) 51, 134, 228, 370, 480
HgaI GACGC 1 cut(s) 391
HhaI GCGC 1 cut(s) 894
Hin1I GRCGYC 1 cut(s) 383
Hin1II CATG 2 cut(s) 64, 460
Hin6I GCGC 1 cut(s) 892
HinP1I GCGC 1 cut(s) 892
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 6 cut(s) 38, 190, 328, 361, 498, 523
HphI GGTGA 2 cut(s) 278, 475
Hpy188I TCNGA 6 cut(s) 184, 327, 360, 471, 528, 608
Hpy188III TCNNGA 9 cut(s) 35, 128, 240, 242, 407, 695, 866, 906, 908
Hpy99I CGWCG 1 cut(s) 385
HpyAV CCTTC 4 cut(s) 332, 409, 567, 655
HpyCH4III ACNGT 1 cut(s) 742
HpyCH4V TGCA 3 cut(s) 756, 791, 832
HpyF10VI GCNNNNNNNGC 3 cut(s) 641, 788, 829
HpyF3I CTNAG 5 cut(s) 468, 525, 605, 723, 747
Hsp92I GRCGYC 1 cut(s) 383
Hsp92II CATG 2 cut(s) 64, 460
HspAI GCGC 1 cut(s) 892
KpnI GGTACC 1 cut(s) 684
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 4 cut(s) 13, 349, 862, 873
LguI GCTCTTC 2 cut(s) 257, 791
LmnI GCTCC 2 cut(s) 184, 779
Lsp1109I GCAGC 2 cut(s) 17, 464
LweI GCATC 2 cut(s) 95, 271
MaeIII GTNAC 4 cut(s) 146, 398, 637, 922
MalI GATC 4 cut(s) 15, 351, 864, 875
MboI GATC 4 cut(s) 13, 349, 862, 873
MboII GAAGA 9 cut(s) 244, 293, 323, 473, 808, 869, 883, 923, 926
MfeI CAATTG 1 cut(s) 335
MflI RGATCY 1 cut(s) 862
MhlI GDGCHC 2 cut(s) 252, 584
MluCI AATT 6 cut(s) 335, 392, 505, 529, 611, 685
MlyI GAGTC 2 cut(s) 199, 517
MmeI TCCRAC 1 cut(s) 114
MroXI GAANNNNTTC 1 cut(s) 123
MseI TTAA 2 cut(s) 711, 976
MslI CAYNNNNRTG 1 cut(s) 65
MspR9I CCNGG 1 cut(s) 387
MunI CAATTG 1 cut(s) 335
MvaI CCWGG 1 cut(s) 387
MwoI GCNNNNNNNGC 3 cut(s) 641, 788, 829
NcoI CCATGG 1 cut(s) 456
NdeII GATC 4 cut(s) 13, 349, 862, 873
NlaIII CATG 2 cut(s) 64, 460
NlaIV GGNNCC 3 cut(s) 661, 670, 682
NmeAIII GCCGAG 1 cut(s) 204
NmuCI GTSAC 1 cut(s) 637
PaeR7I CTCGAG 4 cut(s) 128, 240, 407, 906
PaqCI CACCTGC 1 cut(s) 823
PciSI GCTCTTC 2 cut(s) 257, 791
PdmI GAANNNNTTC 1 cut(s) 123
PfeI GAWTC 4 cut(s) 38, 328, 361, 498
PflMI CCANNNNNTGG 1 cut(s) 270
PkrI GCNGC 3 cut(s) 7, 53, 454
PleI GAGTC 2 cut(s) 198, 517
PpsI GAGTC 2 cut(s) 198, 517
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 385
PspEI GGTNACC 1 cut(s) 146
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 3 cut(s) 661, 670, 682
PspPI GGNCC 4 cut(s) 57, 369, 418, 935
PstNI CAGNNNCTG 2 cut(s) 11, 193
PsuI RGATCY 1 cut(s) 862
RsaI GTAC 2 cut(s) 176, 682
RsaNI GTAC 2 cut(s) 175, 681
RseI CAYNNNNRTG 1 cut(s) 65
SacI GAGCTC 1 cut(s) 252
SapI GCTCTTC 2 cut(s) 257, 791
SaqAI TTAA 2 cut(s) 711, 976
SatI GCNGC 3 cut(s) 6, 52, 453
Sau3AI GATC 4 cut(s) 13, 349, 862, 873
Sau96I GGNCC 4 cut(s) 57, 369, 418, 935
SchI GAGTC 2 cut(s) 199, 517
ScrFI CCNGG 1 cut(s) 387
SduI GDGCHC 2 cut(s) 252, 584
SfaNI GCATC 2 cut(s) 95, 271
Sfr274I CTCGAG 4 cut(s) 128, 240, 407, 906
SinI GGWCC 3 cut(s) 57, 418, 935
SlaI CTCGAG 4 cut(s) 128, 240, 407, 906
SmiMI CAYNNNNRTG 1 cut(s) 65
SmlI CTYRAG 5 cut(s) 128, 240, 407, 866, 906
SmoI CTYRAG 5 cut(s) 128, 240, 407, 866, 906
Sse9I AATT 6 cut(s) 335, 392, 505, 529, 611, 685
SsiI CCGC 2 cut(s) 52, 151
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 1 cut(s) 385
StyI CCWWGG 1 cut(s) 456
TaaI ACNGT 1 cut(s) 742
TaqI TCGA 8 cut(s) 129, 159, 241, 408, 428, 696, 840, 907
TasI AATT 6 cut(s) 335, 392, 505, 529, 611, 685
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 4 cut(s) 38, 328, 361, 498
Tru1I TTAA 2 cut(s) 711, 976
Tru9I TTAA 2 cut(s) 711, 976
TscAI CASTG 3 cut(s) 371, 382, 798
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 2 cut(s) 5, 452
Tsp45I GTSAC 1 cut(s) 637
TspDTI ATGAA 3 cut(s) 103, 244, 729
TspRI CASTG 3 cut(s) 371, 382, 798
Van91I CCANNNNNTGG 1 cut(s) 270
VpaK11BI GGWCC 3 cut(s) 57, 418, 935
XapI RAATTY 2 cut(s) 529, 611
XhoI CTCGAG 4 cut(s) 128, 240, 407, 906
XmnI GAANNNNTTC 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.