Rorug04G0184000

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0017428
Physical Location & Seq
Forward (+)
32100510 .. 32106055
5546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0184000.1

Sequence Viewer

Length: 3372 bp
ATGGCTTCTCTGACCCCTGAAGCAGCAGTCAGTTCATCATCTTCTTCTCTCCCACGTTCTTCTACTGATCACCAAACTCATTACACATACGAGGTGTTCCTGAGTTTTCGAGGCGAGGATACACGCTTTAATTTTACAGATCATTTGCACACCGCTTTGTGTGAGAGGGGAATTAAGACCTTCATAGATGATGAGGGGCTTAGAAGAGGAGAAGAAATATCACAAGACCTCCTCAAAGCAATTGAGGAGTCGAGAGTTTCAATCATTGTCTTGTCTCAAAATTATGCATCATCAAGGTGGTGTTTGAATGAACTGGTCAGGATACTTGAATGCAGAAAATCAAAAGGACAAGAGGTTAGACCAGTTTTCTACAAGGTGGATCCCTCAGATGTACGACACCAAAGAGGTGCTTTTGGTGACGCATTTGCTAAACTTGATCAATGCAAATACAAGGATAGCATGGAAAAATGGAAGGCAGCTCTTACGGAAATAGCAGATTTGTCTGGATGGCCTTTCAAGGATGGCGAGTATGAGGCTAAATTTATCAAAAAGATTGTTGGGGAGTTGTCCGCCCGAGTAGTAAACCCTTCATGTGTGTTGCAAGTAGCTGCACATCCAATTGGAATAGAGTCTTGTAGGAAAAATGTCAACAGAGTTTTAGATGCCAAGGAAAATATTCGCATGATAGGCATATGGGGACCTGGAGGAATAGGAAAGACAACAATTGCGAAACATATGTTTAATTCAGCTCGCCATAAGTTTGAAGGTAGTTGTTTCTTGGCAGATGTTAGATCTAATTCAAATGGCCTAGCCCAATTGCAAGAGAGACTTCTGTTTGATATTTTAAGGGACTCAACTTTGAAGGTGCGCAGTGTTGATGAAGGAGTCAGCTTAATAAAGGCAAGGATGCGACATAAAACAGTTCTCTTAATCCTTGATGACGTCAGTCATTCCAACCAATTACAGAACTTAGCTCCATCCTTTGATTGTTTTGGGCCGGGCAGTAGAATTATTATAACAACAAGGGATAAGCGTTCGCTAACAGCTCATCAAGTTGATGAAGTATACGAGGTCAAAATGTTAGATGATGATCAAGCTTTGGAGCTGTTTAGCTTGAATGCATTCAAAAGAAATGGACCTCCAGCCAATTATCTAGAACTTGCACACCGTGCAGTGCGCTATGCCGGAGGCCTTCCGTTAGCTTTGACAGTTTTAGGCTCTCATCTGTTTCGTAGAAGCAGAGATGAGTGGGAAGCTACATTAGATAGTTGTAAAGGAGAAGATCCCCACATAAAGATAAGAAATGTTCTCAAAATAAGTTATGATGCTCTGGCAGTAGATTTGAAAGGATATTTTCTTGATATCGCATGTTTCTTTAAAGGTGAGCATGTAGACCTTGTGAAACCAATACTAGAAGCTTGCTACGACCTCAAATCAGTGACTGGTATTGCACAACTCCAAGAAAAGGCTCTAATACGAATAGACGGAAATAAGATTTGGATGCATGACTTGATAGAAGAAATGGGTAAAGACATAGTGTATCAAGAGTCACCTGGTGAGCCCGGAAAACGCAGCAGAGTGTGGACTGAAGAAGATGTCGACCACATTCTAACAAATAATACAGGAACAAAAAAAGTTATAGGCATCCAGCTCCCGTTGCGATCATCTACGATATCTTTGAATGCTAAATGTTTCTCAAAGATGAAGAGTCTTAGATATATTTCTATGGGGAAGTACACGAAATATGAATATTTTTCTGGAGATATTGATTATCTCTCCAACCAGTTGAGGTGGCTTGATTGGCCTGATAGTCCGTTGCAATCTCTTCCATCCGATTTTCATGCAAATAGACTTGTAAGGCTCAATATTCCTAACAACCGCGGAATCACACGACTATGGGAGGGACATAAGAATTTTTTAAGGCTAACATATATGAATTTAAGGGGTTGTGAATCTCTAACAGAACTCCCAGACTTCAGTGGAATCCCCAACTTGAAAGAGTTGAATCTATCTGGATGTAGCAGTTTAGTTAAGGTTCCTGATTCCGTTGGATTGCTTGATAAGCTTGTTACCTTGAATACTCACTTATGCTACAACCTTATTATGTTTCCTAGAGAAATCAAGTTGAAATCCTTAGAAACTCTGTGTATTAGCTGTTGCAGTAGGCTTGAGGGATTTTCAGAAGTAACGGAAGAGATGGATTCCTTAAGGAATTTTGATCTATCATTCAGTTTCATGAAAGAATTGCATCCGTCCGTTAAAAAACTGATTGGGCTAGAAGAGCTGAATCTAGAGTATTGTCGAAATCTTAGAACTCTACCGTATACCATTTATGAGTTGCGCAATCTAAAGTATCTTTATGCGAGTAATTGCTCAAGTCTTGCTACATTTCCAAAAATTCCAATAAAGATGGATTCAATGAGATCACTTTATCTTTCAGGCAGTGACATCAGAGAATTGGATCAGTCAATTGGAAATCTGATTGGTCTTAGAGTGTTGAATCTTAGTGATTGCAACAATCTTACAACTCTACCGTGCAGCATTTATGGGTTGCAAAATTTAGAGAAGCTTGATCTTACTGAGTGCTCAAGACTGGTTAGATTTCCAACAAATACCAACATTTTGAATGTCGTTGATAGCTCACCATCACTTCCCAAGCTAGAGTGGTTCAGTATCAGAGGGTGCAATTTATCAGATTGTGATTTCCTCACGACTCTTGATTGCTGGGAAACATTAGAGTACCTTGATCTCTCAGGAAACAATTTTGTTAGTCTTCCTGCTTGCTTCACCAAATGTGTCAACTTGCGGTTGTTTTACTTGGAGGTTGATCCCACTGTGTGGGAGAATATTGGTGGCAGTCAGCTAGGCACTGCAAACCAAGTGGGAACTATAAACTTTCACTTGGAATTGTTGCAGATTCGAGAAAAACGGGTACATGCTGATCTGACTGAATTTGAGACTATTGCAAATAAGCTAGAAGTAAAGATTCATGCAAGCAAGTTCGTGCTGTTTATATCAAATGAACCCCACCTAGATTTATCCACTGATGGATTTTACCTATTTCAAATTTGGAAGGTTACGAGCAAAAATACAGTTGTATACCTTGACGTTGGCGAGACTCTTATGATGGGCAGAGCAAGCAATGAGCGATATCTAAATAGACCACTTAGAAGATTGGTATTCATGCTTCTGTCAAACATATATGCATTAGCTGGGAAATGTGCTGATGTTGCAGAAGTGAGGCTACATTTAAAAGAGAGAGGGATCCGGAAGGTGCCTGGATCAAGTTCAATTGAAGTTAATGGAATGATCCATGACTTTACCTCTGGCGGTGATACTGATGCGCAGACAGAGAAAGCCAGATTGCGTTGA

Protein Analysis

1123

Amino Acids

127.32

Weight (kDa)

6.84

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 30 - 192 2.1e-56 TIR domain
TIR_2 PF13676 32 - 128 1.4e-16 TIR domain
NB-ARC PF00931 215 - 377 1.1e-22 NB-ARC domain
WHD_ROQ1 PF23282 449 - 517 6.9e-13 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 637 - 799 5.9e-09 Leucine-rich repeat region
LRR_13 PF23286 709 - 794 4.4e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 783 - 935 1.2e-17 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_8 PF13855 806 - 861 5.3e-06 Leucine rich repeat
LRR_14 PF23598 806 - 869 3e-06 Leucine-rich repeat region
E_motif PF20431 1061 - 1099 1.2e-08 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1016
AatII GACGTC 1 cut(s) 945
Acc16I TGCGCA 3 cut(s) 869, 2342, 3345
AccB1I GGYRCC 1 cut(s) 3274
AccB7I CCANNNNNTGG 1 cut(s) 2838
AccI GTMKAC 5 cut(s) 1065, 1392, 1599, 2324, 3099
AccII CGCG 1 cut(s) 1881
AccIII TCCGGA 1 cut(s) 3267
AciI CCGC 6 cut(s) 153, 570, 1879, 1881, 2806, 3330
AclWI GGATC 9 cut(s) 374, 387, 1277, 2469, 2822, 3259, 3272, 3289, 3304
AcsI RAATTY 8 cut(s) 539, 1912, 1936, 2212, 2397, 2557, 2951, 3066
AcuI CTGAAG 3 cut(s) 39, 1608, 1960
AcyI GRCGYC 1 cut(s) 942
AdeI CACNNNGTG 3 cut(s) 1169, 1556, 2838
AfaI GTAC 4 cut(s) 393, 1736, 2741, 2934
AfiI CCNNNNNNNGG 2 cut(s) 1465, 2838
AflII CTTAAG 1 cut(s) 2206
AjnI CCWGG 3 cut(s) 700, 1552, 3277
AjuI GAANNNNNNNTTGG 2 cut(s) 1480, 1512
Alw21I GWGCWC 1 cut(s) 2588
Alw26I GTCTC 4 cut(s) 279, 820, 2951, 3110
AlwI GGATC 9 cut(s) 374, 387, 1277, 2469, 2822, 3259, 3272, 3289, 3304
AlwNI CAGNNNCTG 1 cut(s) 1442
Ama87I CYCGRG 1 cut(s) 573
Aor13HI TCCGGA 1 cut(s) 3267
AoxI GGCC 5 cut(s) 509, 805, 995, 1189, 1802
ApeKI GCWGC 5 cut(s) 23, 476, 608, 1572, 2538
ApoI RAATTY 8 cut(s) 539, 1912, 1936, 2212, 2397, 2557, 2951, 3066
Asp700I GAANNNNTTC 2 cut(s) 675, 1121
AspLEI GCGC 4 cut(s) 870, 1179, 2343, 3346
AspS9I GGNCC 3 cut(s) 698, 995, 1136
AsuC2I CCSGG 2 cut(s) 999, 1563
AsuHPI GGTGA 8 cut(s) 62, 428, 1394, 1542, 1568, 2634, 2779, 3344
AvaI CYCGRG 1 cut(s) 573
AvaII GGWCC 2 cut(s) 698, 1136
BamHI GGATCC 2 cut(s) 379, 3264
BanI GGYRCC 1 cut(s) 3274
BanII GRGCYC 1 cut(s) 1563
BarI GAAGNNNNNNTAC 4 cut(s) 3171, 3203, 3228, 3260
BbsI GAAGAC 1 cut(s) 2765
Bbv12I GWGCWC 1 cut(s) 2588
BbvI GCAGC 5 cut(s) 35, 488, 595, 1584, 2550
BccI CCATC 9 cut(s) 501, 515, 985, 1837, 2191, 2404, 2653, 3041, 3121
BcgI CGANNNNNNTGC 2 cut(s) 1823, 1857
BciT130I CCWGG 3 cut(s) 702, 1554, 3279
BciVI GTATCC 2 cut(s) 112, 315
BclI TGATCA 3 cut(s) 67, 436, 1090
BcnI CCSGG 2 cut(s) 999, 1563
BcoDI GTCTC 4 cut(s) 279, 820, 2951, 3110
BfrI CTTAAG 1 cut(s) 2206
BfuI GTATCC 2 cut(s) 112, 315
BglII AGATCT 1 cut(s) 791
BisI GCNGC 5 cut(s) 24, 477, 609, 1573, 2539
BlsI GCNGC 5 cut(s) 25, 478, 610, 1574, 2540
Bme1390I CCNGG 5 cut(s) 702, 999, 1554, 1563, 3279
Bme18I GGWCC 2 cut(s) 698, 1136
BmeT110I CYCGRG 1 cut(s) 573
BmgT120I GGNCC 3 cut(s) 698, 995, 1136
BmiI GGNNCC 5 cut(s) 381, 699, 2037, 3266, 3276
BmrFI CCNGG 5 cut(s) 702, 999, 1554, 1563, 3279
BmsI GCATC 8 cut(s) 296, 652, 897, 1315, 1491, 1653, 2257, 3331
BoxI GACNNNNGTC 1 cut(s) 945
BpiI GAAGAC 1 cut(s) 2765
BpmI CTGGAG 3 cut(s) 723, 1125, 1779
BpuEI CTTGAG 3 cut(s) 2189, 2359, 2572
BpuMI CCSGG 2 cut(s) 999, 1563
BsaBI GATNNNNATC 1 cut(s) 1089
BsaHI GRCGYC 1 cut(s) 942
BsaJI CCNNGG 2 cut(s) 666, 1879
BsaWI WCCGGW 1 cut(s) 3267
BsaXI ACNNNNNCTCC 2 cut(s) 213, 243
Bsc4I CCNNNNNNNGG 2 cut(s) 1465, 2838
Bse1I ACTGG 5 cut(s) 318, 362, 1447, 1783, 2598
Bse3DI GCAATG 1 cut(s) 3148
Bse8I GATNNNNATC 1 cut(s) 1089
BseAI TCCGGA 1 cut(s) 3267
BseBI CCWGG 3 cut(s) 702, 1554, 3279
BseDI CCNNGG 2 cut(s) 666, 1879
BseJI GATNNNNATC 1 cut(s) 1089
BseLI CCNNNNNNNGG 2 cut(s) 1465, 2838
BseMI GCAATG 1 cut(s) 3148
BseMII CTCAG 4 cut(s) 92, 399, 2571, 2766
BseNI ACTGG 5 cut(s) 318, 362, 1447, 1783, 2598
BseRI GAGGAG 3 cut(s) 221, 222, 260
BseXI GCAGC 5 cut(s) 35, 488, 595, 1584, 2550
BseYI CCCAGC 2 cut(s) 2724, 3212
BsgI GTGCAG 3 cut(s) 594, 1191, 2557
Bsh1236I CGCG 1 cut(s) 1881
BshFI GGCC 5 cut(s) 511, 807, 997, 1191, 1804
BshNI GGYRCC 1 cut(s) 3274
BsiHKAI GWGCWC 1 cut(s) 2588
BsiHKCI CYCGRG 1 cut(s) 573
BsiSI CCGG 4 cut(s) 998, 1185, 1563, 3268
BslFI GGGAC 3 cut(s) 711, 863, 1917
BslI CCNNNNNNNGG 2 cut(s) 1465, 2838
BsmAI GTCTC 4 cut(s) 279, 820, 2951, 3110
BsmFI GGGAC 3 cut(s) 711, 863, 1917
BsmI GAATGC 4 cut(s) 335, 1121, 1123, 1687
BsnI GGCC 5 cut(s) 511, 807, 997, 1191, 1804
BsoBI CYCGRG 1 cut(s) 573
Bsp1286I GDGCHC 2 cut(s) 1563, 2588
Bsp13I TCCGGA 1 cut(s) 3267
BspACI CCGC 6 cut(s) 153, 570, 1879, 1881, 2806, 3330
BspANI GGCC 5 cut(s) 511, 807, 997, 1191, 1804
BspCNI CTCAG 4 cut(s) 93, 398, 2572, 2765
BspEI TCCGGA 1 cut(s) 3267
BspFNI CGCG 1 cut(s) 1881
BspHI TCATGA 1 cut(s) 2235
BspLI GGNNCC 5 cut(s) 381, 699, 2037, 3266, 3276
BspPI GGATC 9 cut(s) 374, 387, 1277, 2469, 2822, 3259, 3272, 3289, 3304
BspQI GCTCTTC 1 cut(s) 2274
BspT107I GGYRCC 1 cut(s) 3274
BspTI CTTAAG 1 cut(s) 2206
BsrDI GCAATG 1 cut(s) 3148
BsrI ACTGG 5 cut(s) 318, 362, 1447, 1783, 2598
BssECI CCNNGG 2 cut(s) 666, 1879
BssNAI GTATAC 3 cut(s) 1066, 2325, 3100
BssNI GRCGYC 1 cut(s) 942
BssT1I CCWWGG 1 cut(s) 666
Bst1107I GTATAC 3 cut(s) 1066, 2325, 3100
Bst2UI CCWGG 3 cut(s) 702, 1554, 3279
Bst4CI ACNGT 7 cut(s) 922, 1169, 1210, 2322, 2535, 2836, 3094
Bst6I CTCTTC 5 cut(s) 199, 1700, 1830, 2187, 2274
BstACI GRCGYC 1 cut(s) 942
BstAFI CTTAAG 1 cut(s) 2206
BstAPI GCANNNNNTGC 1 cut(s) 1169
BstC8I GCNNGC 5 cut(s) 751, 1420, 2782, 2995, 3139
BstDSI CCRYGG 1 cut(s) 1879
BstFNI CGCG 1 cut(s) 1881
BstHHI GCGC 4 cut(s) 870, 1179, 2343, 3346
BstMAI GTCTC 4 cut(s) 279, 820, 2951, 3110
BstMWI GCNNNNNNNGC 8 cut(s) 687, 1169, 1657, 1801, 2062, 2281, 3138, 3230
BstNI CCWGG 3 cut(s) 702, 1554, 3279
BstNSI RCATGY 3 cut(s) 1371, 1391, 2939
BstPAI GACNNNNGTC 1 cut(s) 945
BstSCI CCNGG 5 cut(s) 700, 997, 1552, 1561, 3277
BstUI CGCG 1 cut(s) 1881
BstV1I GCAGC 5 cut(s) 35, 488, 595, 1584, 2550
BstV2I GAAGAC 1 cut(s) 2765
BstX2I RGATCY 4 cut(s) 379, 791, 1282, 3264
BstYI RGATCY 4 cut(s) 379, 791, 1282, 3264
BstZ17I GTATAC 3 cut(s) 1066, 2325, 3100
BsuI GTATCC 2 cut(s) 112, 315
BsuRI GGCC 5 cut(s) 511, 807, 997, 1191, 1804
BtgI CCRYGG 1 cut(s) 1879
BtsI GCAGTG 4 cut(s) 877, 1179, 2449, 2868
BtsIMutI CAGTG 8 cut(s) 877, 1179, 1443, 1984, 2449, 2832, 2868, 3042
Cac8I GCNNGC 5 cut(s) 751, 1420, 2782, 2995, 3139
CaiI CAGNNNCTG 1 cut(s) 1442
CciI TCATGA 1 cut(s) 2235
CfoI GCGC 4 cut(s) 870, 1179, 2343, 3346
Cfr13I GGNCC 3 cut(s) 698, 995, 1136
Cfr42I CCGCGG 1 cut(s) 1882
CseI GACGC 1 cut(s) 428
CsiI ACCWGGT 1 cut(s) 1552
Csp6I GTAC 4 cut(s) 392, 1735, 2740, 2933
CspCI CAANNNNNGTGG 2 cut(s) 2862, 2897
CviQI GTAC 4 cut(s) 392, 1735, 2740, 2933
DraI TTTAAA 2 cut(s) 1378, 3252
DraIII CACNNNGTG 3 cut(s) 1169, 1556, 2838
Eam1104I CTCTTC 5 cut(s) 199, 1700, 1830, 2187, 2274
EarI CTCTTC 5 cut(s) 199, 1700, 1830, 2187, 2274
EciI GGCGGA 1 cut(s) 559
Eco130I CCWWGG 1 cut(s) 666
Eco147I AGGCCT 1 cut(s) 1191
Eco24I GRGCYC 1 cut(s) 1563
Eco32I GATATC 3 cut(s) 1363, 1674, 3152
Eco47I GGWCC 2 cut(s) 698, 1136
Eco57I CTGAAG 3 cut(s) 39, 1608, 1960
Eco88I CYCGRG 1 cut(s) 573
EcoO109I RGGNCCY 1 cut(s) 698
EcoRII CCWGG 3 cut(s) 700, 1552, 3277
EcoRV GATATC 3 cut(s) 1363, 1674, 3152
EcoT14I CCWWGG 1 cut(s) 666
EcoT22I ATGCAT 4 cut(s) 289, 1123, 1506, 3208
EcoT38I GRGCYC 1 cut(s) 1563
ErhI CCWWGG 1 cut(s) 666
FalI AAGNNNNNCTT 4 cut(s) 394, 426, 813, 845
FaqI GGGAC 3 cut(s) 711, 863, 1917
FauNDI CATATG 2 cut(s) 692, 735
FbaI TGATCA 3 cut(s) 67, 436, 1090
FblI GTMKAC 5 cut(s) 1065, 1392, 1599, 2324, 3099
Fnu4HI GCNGC 5 cut(s) 24, 477, 609, 1573, 2539
FriOI GRGCYC 1 cut(s) 1563
Fsp4HI GCNGC 5 cut(s) 24, 477, 609, 1573, 2539
FspI TGCGCA 3 cut(s) 869, 2342, 3345
GlaI GCGC 4 cut(s) 869, 1178, 2342, 3345
GluI GCNGC 5 cut(s) 24, 477, 609, 1573, 2539
GsaI CCCAGC 2 cut(s) 2728, 3216
GsuI CTGGAG 3 cut(s) 723, 1125, 1779
HaeIII GGCC 5 cut(s) 511, 807, 997, 1191, 1804
HapII CCGG 4 cut(s) 998, 1185, 1563, 3268
HgaI GACGC 1 cut(s) 428
HhaI GCGC 4 cut(s) 870, 1179, 2343, 3346
Hin1I GRCGYC 1 cut(s) 942
Hin6I GCGC 4 cut(s) 868, 1177, 2341, 3344
HinP1I GCGC 4 cut(s) 868, 1177, 2341, 3344
HincII GTYRAC 3 cut(s) 649, 1600, 2800
HindII GTYRAC 3 cut(s) 649, 1600, 2800
HindIII AAGCTT 4 cut(s) 1095, 1416, 2063, 2567
HpaII CCGG 4 cut(s) 998, 1185, 1563, 3268
HphI GGTGA 8 cut(s) 62, 428, 1394, 1542, 1568, 2634, 2779, 3344
Hpy188I TCNGA 9 cut(s) 12, 388, 1834, 2182, 2453, 2481, 2678, 2695, 2946
HpyAV CCTTC 9 cut(s) 190, 466, 597, 758, 856, 875, 1202, 3067, 3265
HpyCH4III ACNGT 7 cut(s) 922, 1169, 1210, 2322, 2535, 2836, 3094
HpyCH4IV ACGT 3 cut(s) 55, 942, 3108
HpyF10VI GCNNNNNNNGC 8 cut(s) 687, 1169, 1657, 1801, 2062, 2281, 3138, 3230
HpySE526I ACGT 3 cut(s) 55, 942, 3108
Hsp92I GRCGYC 1 cut(s) 942
HspAI GCGC 4 cut(s) 868, 1177, 2341, 3344
Kpn2I TCCGGA 1 cut(s) 3267
Ksp22I TGATCA 3 cut(s) 67, 436, 1090
KspI CCGCGG 1 cut(s) 1882
LguI GCTCTTC 1 cut(s) 2274
LmnI GCTCC 3 cut(s) 979, 1102, 1656
Lsp1109I GCAGC 5 cut(s) 35, 488, 595, 1584, 2550
LweI GCATC 8 cut(s) 296, 652, 897, 1315, 1491, 1653, 2257, 3331
MabI ACCWGGT 1 cut(s) 1552
MaeII ACGT 3 cut(s) 55, 942, 3108
MaeIII GTNAC 7 cut(s) 416, 1438, 1548, 2068, 2185, 2444, 3076
MfeI CAATTG 6 cut(s) 240, 618, 723, 815, 2469, 3291
MflI RGATCY 4 cut(s) 379, 791, 1282, 3264
MhlI GDGCHC 2 cut(s) 1563, 2588
MlyI GAGTC 8 cut(s) 257, 638, 845, 894, 1556, 1717, 2707, 3112
MmeI TCCRAC 4 cut(s) 978, 1803, 2029, 2630
Mph1103I ATGCAT 4 cut(s) 289, 1123, 1506, 3208
MroI TCCGGA 1 cut(s) 3267
MroXI GAANNNNTTC 2 cut(s) 675, 1121
MslI CAYNNNNRTG 2 cut(s) 295, 1894
MspA1I CMGCKG 1 cut(s) 1881
MspCI CTTAAG 1 cut(s) 2206
MspI CCGG 4 cut(s) 998, 1185, 1563, 3268
MspR9I CCNGG 5 cut(s) 702, 999, 1554, 1563, 3279
MunI CAATTG 6 cut(s) 240, 618, 723, 815, 2469, 3291
Mva1269I GAATGC 4 cut(s) 335, 1121, 1123, 1687
MvaI CCWGG 3 cut(s) 702, 1554, 3279
MvnI CGCG 1 cut(s) 1881
MwoI GCNNNNNNNGC 8 cut(s) 687, 1169, 1657, 1801, 2062, 2281, 3138, 3230
NciI CCSGG 2 cut(s) 999, 1563
NdeI CATATG 2 cut(s) 692, 735
NlaIV GGNNCC 5 cut(s) 381, 699, 2037, 3266, 3276
NmuCI GTSAC 4 cut(s) 416, 1438, 1548, 2444
NsbI TGCGCA 3 cut(s) 869, 2342, 3345
NsiI ATGCAT 4 cut(s) 289, 1123, 1506, 3208
NspI RCATGY 3 cut(s) 1371, 1391, 2939
PagI TCATGA 1 cut(s) 2235
PceI AGGCCT 1 cut(s) 1191
PciSI GCTCTTC 1 cut(s) 2274
PctI GAATGC 4 cut(s) 335, 1121, 1123, 1687
PdmI GAANNNNTTC 2 cut(s) 675, 1121
PflMI CCANNNNNTGG 1 cut(s) 2838
PkrI GCNGC 5 cut(s) 25, 478, 610, 1574, 2540
PleI GAGTC 8 cut(s) 256, 637, 845, 893, 1555, 1716, 2707, 3112
PpsI GAGTC 8 cut(s) 256, 637, 845, 893, 1555, 1716, 2707, 3112
PpuMI RGGWCCY 1 cut(s) 698
PshAI GACNNNNGTC 1 cut(s) 945
PsiI TTATAA 1 cut(s) 1016
Psp5II RGGWCCY 1 cut(s) 698
Psp6I CCWGG 3 cut(s) 700, 1552, 3277
PspFI CCCAGC 2 cut(s) 2724, 3212
PspGI CCWGG 3 cut(s) 700, 1552, 3277
PspN4I GGNNCC 5 cut(s) 381, 699, 2037, 3266, 3276
PspPI GGNCC 3 cut(s) 698, 995, 1136
PspPPI RGGWCCY 1 cut(s) 698
PstNI CAGNNNCTG 1 cut(s) 1442
PsuI RGATCY 4 cut(s) 379, 791, 1282, 3264
RsaI GTAC 4 cut(s) 393, 1736, 2741, 2934
RsaNI GTAC 4 cut(s) 392, 1735, 2740, 2933
RseI CAYNNNNRTG 2 cut(s) 295, 1894
SacII CCGCGG 1 cut(s) 1882
SalI GTCGAC 1 cut(s) 1598
SapI GCTCTTC 1 cut(s) 2274
SatI GCNGC 5 cut(s) 24, 477, 609, 1573, 2539
Sau96I GGNCC 3 cut(s) 698, 995, 1136
SchI GAGTC 8 cut(s) 257, 638, 845, 894, 1556, 1717, 2707, 3112
ScrFI CCNGG 5 cut(s) 702, 999, 1554, 1563, 3279
SduI GDGCHC 2 cut(s) 1563, 2588
SexAI ACCWGGT 1 cut(s) 1552
SfaNI GCATC 8 cut(s) 296, 652, 897, 1315, 1491, 1653, 2257, 3331
Sfr303I CCGCGG 1 cut(s) 1882
SgrBI CCGCGG 1 cut(s) 1882
SinI GGWCC 2 cut(s) 698, 1136
SmiMI CAYNNNNRTG 2 cut(s) 295, 1894
SmlI CTYRAG 4 cut(s) 2168, 2206, 2374, 2587
SmoI CTYRAG 4 cut(s) 2168, 2206, 2374, 2587
SseBI AGGCCT 1 cut(s) 1191
SsiI CCGC 6 cut(s) 153, 570, 1879, 1881, 2806, 3330
SspI AATATT 4 cut(s) 676, 1751, 1867, 2848
StuI AGGCCT 1 cut(s) 1191
StyD4I CCNGG 5 cut(s) 700, 997, 1552, 1561, 3277
StyI CCWWGG 1 cut(s) 666
TaaI ACNGT 7 cut(s) 922, 1169, 1210, 2322, 2535, 2836, 3094
TaiI ACGT 3 cut(s) 58, 945, 3111
TaqI TCGA 5 cut(s) 109, 251, 1599, 2302, 2920
TatI WGTACW 1 cut(s) 1734
TscAI CASTG 8 cut(s) 877, 1179, 1443, 1984, 2449, 2839, 2875, 3049
TseFI GTSAC 4 cut(s) 416, 1438, 1548, 2444
TseI GCWGC 5 cut(s) 23, 476, 608, 1572, 2538
Tsp45I GTSAC 4 cut(s) 416, 1438, 1548, 2444
TspGWI ACGGA 8 cut(s) 500, 1185, 1500, 1803, 2035, 2204, 2241, 2245
TspRI CASTG 8 cut(s) 877, 1179, 1443, 1984, 2449, 2839, 2875, 3049
Van91I CCANNNNNTGG 1 cut(s) 2838
Vha464I CTTAAG 1 cut(s) 2206
VpaK11BI GGWCC 2 cut(s) 698, 1136
XapI RAATTY 8 cut(s) 539, 1912, 1936, 2212, 2397, 2557, 2951, 3066
XbaI TCTAGA 2 cut(s) 1153, 2290
XceI RCATGY 3 cut(s) 1371, 1391, 2939
XmiI GTMKAC 5 cut(s) 1065, 1392, 1599, 2324, 3099
XmnI GAANNNNTTC 2 cut(s) 675, 1121
ZraI GACGTC 1 cut(s) 943
Zsp2I ATGCAT 4 cut(s) 289, 1123, 1506, 3208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.