Rorug04G0240100

Protein kinase APK1B, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0016828
Physical Location & Seq
Reverse (-)
40834611 .. 40834916
306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0240100.1

Sequence Viewer

Length: 306 bp
ATGAGGATATTTGTTGCTTCGGAGCTTCTGGAATTGCCCAAAAAGGACAAGCGCCGCCTCCTCCACGCCGTGTACCGCGTCGGCGATCTCGAGCGCACCATCAAGTTTTACACTGAGGCTCTTGGGATGAAGCTGTTGCGGCAGAGGGATATTCCGGAAGAGAAGTACTCGAATGCTTTCCTCGGATTCGGGCAGGAAGAAACGAACTTTGTAATGGAGCTCACTTACAATTATGGAGTAATTCCTATGATATTGGGATTGAGTTTGGTTACAAAAGAGCTTGGAGGAAAAATAACAAGAGAGTAG

Protein Analysis

101

Amino Acids

11.74

Weight (kDa)

8.92

Isoelectric Point (pI)

29.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 19 - 67 7.3e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016828)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23630
malus_domestica MD13G1120500.v1.1
prunus_persica Prupe.1G224700_v2.0.a1
pyrus_communis pycom13g10490
rosa_chinensis RchiOBHm_Chr4g0430091
rosa_laevigata RLG00000007004
rosa_multiflora Rmu_ssc0000150.1_g000025
rosa_roxburghii Rroxscaffold_5G00371910
rosa_rugosa Rorug04G0240100
rosa_samantha Rh4AG297200 Rh4BG303600 Rh4CG319200 Rh4DG301100
rosa_wichuraiana Rw4G025770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 78
AccIII TCCGGA 1 cut(s) 154
AciI CCGC 3 cut(s) 55, 76, 139
AdeI CACNNNGTG 1 cut(s) 70
AfaI GTAC 2 cut(s) 74, 167
AluBI AGCT 4 cut(s) 25, 133, 220, 280
AluI AGCT 4 cut(s) 25, 133, 220, 280
Alw21I GWGCWC 1 cut(s) 222
Ama87I CYCGRG 1 cut(s) 89
Aor13HI TCCGGA 1 cut(s) 154
Asp700I GAANNNNTTC 1 cut(s) 176
AspLEI GCGC 2 cut(s) 54, 96
AvaI CYCGRG 1 cut(s) 89
BanII GRGCYC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 222
BccI CCATC 1 cut(s) 107
BceAI ACGGC 1 cut(s) 53
BfoI RGCGCY 1 cut(s) 55
BisI GCNGC 2 cut(s) 55, 140
BlsI GCNGC 2 cut(s) 56, 141
BmcAI AGTACT 1 cut(s) 167
BmeT110I CYCGRG 1 cut(s) 89
BplI GAGNNNNNCTC 2 cut(s) 152, 184
BsaJI CCNNGG 1 cut(s) 181
BsaWI WCCGGW 1 cut(s) 154
BseAI TCCGGA 1 cut(s) 154
BseDI CCNNGG 1 cut(s) 181
BseGI GGATG 1 cut(s) 132
BseMII CTCAG 1 cut(s) 105
BseRI GAGGAG 1 cut(s) 50
Bsh1236I CGCG 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 222
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 1 cut(s) 155
BsmI GAATGC 1 cut(s) 178
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 222
Bsp13I TCCGGA 1 cut(s) 154
Bsp143I GATC 1 cut(s) 85
BspACI CCGC 3 cut(s) 55, 76, 139
BspCNI CTCAG 1 cut(s) 106
BspEI TCCGGA 1 cut(s) 154
BspFNI CGCG 1 cut(s) 78
BssECI CCNNGG 1 cut(s) 181
BssMI GATC 1 cut(s) 85
Bst6I CTCTTC 1 cut(s) 153
BstDEI CTNAG 1 cut(s) 114
BstF5I GGATG 1 cut(s) 132
BstFNI CGCG 1 cut(s) 78
BstH2I RGCGCY 1 cut(s) 55
BstHHI GCGC 2 cut(s) 54, 96
BstKTI GATC 1 cut(s) 88
BstMBI GATC 1 cut(s) 85
BstMWI GCNNNNNNNGC 1 cut(s) 139
BstUI CGCG 1 cut(s) 78
BtsCI GGATG 1 cut(s) 132
BtsIMutI CAGTG 1 cut(s) 111
CfoI GCGC 2 cut(s) 54, 96
CseI GACGC 1 cut(s) 67
Csp6I GTAC 2 cut(s) 73, 166
CviJI RGCY 5 cut(s) 25, 119, 133, 220, 280
CviKI_1 RGCY 5 cut(s) 25, 119, 133, 220, 280
CviQI GTAC 2 cut(s) 73, 166
DdeI CTNAG 1 cut(s) 114
DpnI GATC 1 cut(s) 87
DpnII GATC 1 cut(s) 85
DraIII CACNNNGTG 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Ecl136II GAGCTC 1 cut(s) 220
Eco24I GRGCYC 1 cut(s) 222
Eco53kI GAGCTC 1 cut(s) 220
Eco88I CYCGRG 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 220
EcoT38I GRGCYC 1 cut(s) 222
FaiI YATR 2 cut(s) 234, 248
Fnu4HI GCNGC 2 cut(s) 55, 140
FokI GGATG 1 cut(s) 139
FriOI GRGCYC 1 cut(s) 222
Fsp4HI GCNGC 2 cut(s) 55, 140
GlaI GCGC 2 cut(s) 53, 95
GluI GCNGC 2 cut(s) 55, 140
HaeII RGCGCY 1 cut(s) 55
HapII CCGG 1 cut(s) 155
HgaI GACGC 1 cut(s) 67
HhaI GCGC 2 cut(s) 54, 96
Hin6I GCGC 2 cut(s) 52, 94
HinP1I GCGC 2 cut(s) 52, 94
HinfI GANTC 1 cut(s) 186
HpaII CCGG 1 cut(s) 155
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 2 cut(s) 22, 185
Hpy188III TCNNGA 3 cut(s) 29, 89, 155
Hpy8I GTNNAC 1 cut(s) 73
Hpy99I CGWCG 1 cut(s) 83
HpyF10VI GCNNNNNNNGC 1 cut(s) 139
HpyF3I CTNAG 1 cut(s) 114
HspAI GCGC 2 cut(s) 52, 94
Kpn2I TCCGGA 1 cut(s) 154
Kzo9I GATC 1 cut(s) 85
LmnI GCTCC 2 cut(s) 22, 217
LpnPI CCDG 3 cut(s) 14, 168, 179
MaeIII GTNAC 1 cut(s) 268
MalI GATC 1 cut(s) 87
MboI GATC 1 cut(s) 85
MboII GAAGA 2 cut(s) 170, 209
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 3 cut(s) 32, 229, 240
MnlI CCTC 6 cut(s) 68, 71, 109, 138, 191, 278
MroI TCCGGA 1 cut(s) 154
MroXI GAANNNNTTC 1 cut(s) 176
MspI CCGG 1 cut(s) 155
Mva1269I GAATGC 1 cut(s) 178
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 1 cut(s) 139
NdeII GATC 1 cut(s) 85
PaeR7I CTCGAG 1 cut(s) 89
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PctI GAATGC 1 cut(s) 178
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 1 cut(s) 186
PkrI GCNGC 2 cut(s) 56, 141
Psp124BI GAGCTC 1 cut(s) 222
RsaI GTAC 2 cut(s) 74, 167
RsaNI GTAC 2 cut(s) 73, 166
SacI GAGCTC 1 cut(s) 222
SatI GCNGC 2 cut(s) 55, 140
Sau3AI GATC 1 cut(s) 85
ScaI AGTACT 1 cut(s) 167
SduI GDGCHC 1 cut(s) 222
SetI ASST 4 cut(s) 27, 135, 222, 282
Sfr274I CTCGAG 1 cut(s) 89
SlaI CTCGAG 1 cut(s) 89
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
Sse9I AATT 3 cut(s) 32, 229, 240
SsiI CCGC 3 cut(s) 55, 76, 139
SstI GAGCTC 1 cut(s) 222
TaqI TCGA 2 cut(s) 90, 170
TasI AATT 3 cut(s) 32, 229, 240
TatI WGTACW 1 cut(s) 165
TauI GCSGC 2 cut(s) 57, 142
TfiI GAWTC 1 cut(s) 186
TscAI CASTG 1 cut(s) 118
TspDTI ATGAA 1 cut(s) 143
TspRI CASTG 1 cut(s) 118
XhoI CTCGAG 1 cut(s) 89
XmnI GAANNNNTTC 1 cut(s) 176
ZrmI AGTACT 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.