Rorug04G0369400

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010562
Physical Location & Seq
Reverse (-)
54057573 .. 54059319
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0369400.1

Sequence Viewer

Length: 735 bp
ATGGCCACAGGTTCAGTAGTCCCATTGCTCTTCCTAGCCAGAATCTCTGGGCTTTTGGTTGCAGTGCTGGTCTCGATTTGGGCTATTTCATTCAGGTCCAGCTTTCATCATCACTCTTCTGAGTCCTCTCCCCAAGAAGACCAGGGCATCACCTATTCAGCTCTTCATCCTTTGTTCACGGTCATTGGCTTTATTCTCATCAGTGGAGAAGCAATTTTGATACATAGGTGGTTGCCTGGTTCAAGAAGTTTGAAGAAATCAGTGCATTTGTGTCTTCAAGGTGTGGCTTTGGCTTCTGGGATCTTTGGGATTTGGACCAAGTTTCATGGCCAGAAGGGCATTGTGGCTAATTTCTATAGTCTTCATTCTTGGATGGGTTTGATCTGCATTCTCTTGTTTGGAGCTCAGTGGTTGGTGGGTTTCTTGAGCTTTTGGCATAGAGGGGAAGGGCGCACGGTAAGGCAAAGGGTGTTGCCCTGGCATATCTTCCTTGGGCTCTACACTTATGGTTTGGCAGTGGCTACAGCAGAAACTGGGCTTTTGGAGAAGTTGACATTCTTACAAACAAATAGGAATGTGTCTAAACACAGCCCAGAGTCCATGGTTGTTAATAGTTTAGGGCTTGGGTTGGCCGTCCTCAGTGGCATTGTAATATTGGCTGCAGTATCACCCAAGTACCAACCCATTCAAACTAAACTCATTTACTCAGATACCAAAAAATGTCCATCCTGTTAA

Protein Analysis

244

Amino Acids

26.78

Weight (kDa)

9.73

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 55 - 186 1.2e-39 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 308
AcoI YGGCCR 3 cut(s) 3, 328, 630
AfaI GTAC 1 cut(s) 677
AgsI TTSAA 4 cut(s) 243, 253, 278, 689
AjnI CCWGG 3 cut(s) 141, 235, 476
AluBI AGCT 4 cut(s) 102, 161, 404, 429
AluI AGCT 4 cut(s) 102, 161, 404, 429
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 1 cut(s) 76
AlwI GGATC 1 cut(s) 308
AlwNI CAGNNNCTG 1 cut(s) 533
AoxI GGCC 3 cut(s) 3, 328, 630
ApeKI GCWGC 1 cut(s) 659
AspLEI GCGC 1 cut(s) 453
AspS9I GGNCC 2 cut(s) 96, 315
AsuHPI GGTGA 2 cut(s) 142, 660
AvaII GGWCC 2 cut(s) 96, 315
BaeI ACNNNNGTAYC 4 cut(s) 212, 245, 659, 692
BalI TGGCCA 2 cut(s) 5, 330
BanII GRGCYC 2 cut(s) 406, 498
BbsI GAAGAC 3 cut(s) 144, 266, 353
Bbv12I GWGCWC 1 cut(s) 406
BbvI GCAGC 1 cut(s) 646
BccI CCATC 2 cut(s) 367, 733
BceAI ACGGC 1 cut(s) 617
BciT130I CCWGG 3 cut(s) 143, 237, 478
BcoDI GTCTC 1 cut(s) 76
BfaI CTAG 1 cut(s) 35
BfmI CTRYAG 3 cut(s) 355, 522, 660
BglI GCCNNNNNGGC 1 cut(s) 336
BisI GCNGC 1 cut(s) 660
BlsI GCNGC 1 cut(s) 661
Bme1390I CCNGG 3 cut(s) 143, 237, 478
Bme18I GGWCC 2 cut(s) 96, 315
BmgT120I GGNCC 2 cut(s) 96, 315
BmrFI CCNGG 3 cut(s) 143, 237, 478
BmrI ACTGGG 1 cut(s) 543
BmsI GCATC 1 cut(s) 156
BmuI ACTGGG 1 cut(s) 543
BpiI GAAGAC 3 cut(s) 144, 266, 353
BpuEI CTTGAG 1 cut(s) 445
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 4 cut(s) 142, 476, 490, 600
Bse1I ACTGG 1 cut(s) 538
Bse3DI GCAATG 1 cut(s) 23
BseBI CCWGG 3 cut(s) 143, 237, 478
BseDI CCNNGG 4 cut(s) 142, 476, 490, 600
BseGI GGATG 3 cut(s) 166, 378, 725
BseMI GCAATG 1 cut(s) 23
BseMII CTCAG 4 cut(s) 111, 419, 652, 720
BseNI ACTGG 1 cut(s) 538
BseXI GCAGC 1 cut(s) 646
BshFI GGCC 3 cut(s) 5, 330, 632
BsiHKAI GWGCWC 1 cut(s) 406
BslFI GGGAC 1 cut(s) 5
BsmAI GTCTC 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 5
BsmI GAATGC 1 cut(s) 387
BsnI GGCC 3 cut(s) 5, 330, 632
Bso31I GGTCTC 1 cut(s) 76
Bsp1286I GDGCHC 2 cut(s) 406, 498
Bsp143I GATC 2 cut(s) 300, 381
Bsp19I CCATGG 1 cut(s) 600
BspANI GGCC 3 cut(s) 5, 330, 632
BspCNI CTCAG 4 cut(s) 112, 418, 651, 719
BspMAI CTGCAG 1 cut(s) 664
BspPI GGATC 1 cut(s) 308
BspQI GCTCTTC 2 cut(s) 35, 168
BspTNI GGTCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 23
BsrI ACTGG 1 cut(s) 538
BssECI CCNNGG 4 cut(s) 142, 476, 490, 600
BssMI GATC 2 cut(s) 300, 381
BssT1I CCWWGG 2 cut(s) 490, 600
Bst2UI CCWGG 3 cut(s) 143, 237, 478
Bst4CI ACNGT 2 cut(s) 181, 457
Bst6I CTCTTC 3 cut(s) 35, 121, 168
BstDEI CTNAG 4 cut(s) 120, 405, 638, 706
BstDSI CCRYGG 1 cut(s) 600
BstF5I GGATG 3 cut(s) 166, 378, 725
BstHHI GCGC 1 cut(s) 453
BstKTI GATC 2 cut(s) 303, 384
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 2 cut(s) 300, 381
BstMWI GCNNNNNNNGC 1 cut(s) 336
BstNI CCWGG 3 cut(s) 143, 237, 478
BstSCI CCNGG 3 cut(s) 141, 235, 476
BstSFI CTRYAG 3 cut(s) 355, 522, 660
BstV1I GCAGC 1 cut(s) 646
BstV2I GAAGAC 3 cut(s) 144, 266, 353
BstX2I RGATCY 1 cut(s) 300
BstYI RGATCY 1 cut(s) 300
BsuRI GGCC 3 cut(s) 5, 330, 632
BtgI CCRYGG 1 cut(s) 600
BtsCI GGATG 3 cut(s) 166, 378, 725
BtsI GCAGTG 2 cut(s) 69, 522
BtsIMutI CAGTG 6 cut(s) 69, 208, 267, 413, 522, 646
CaiI CAGNNNCTG 1 cut(s) 533
CfoI GCGC 1 cut(s) 453
Cfr13I GGNCC 2 cut(s) 96, 315
Csp6I GTAC 1 cut(s) 676
CviAII CATG 2 cut(s) 326, 601
CviQI GTAC 1 cut(s) 676
DdeI CTNAG 4 cut(s) 120, 405, 638, 706
DpnI GATC 2 cut(s) 302, 383
DpnII GATC 2 cut(s) 300, 381
EaeI YGGCCR 3 cut(s) 3, 328, 630
Eam1104I CTCTTC 3 cut(s) 35, 121, 168
EarI CTCTTC 3 cut(s) 35, 121, 168
Ecl136II GAGCTC 1 cut(s) 404
Eco130I CCWWGG 2 cut(s) 490, 600
Eco24I GRGCYC 2 cut(s) 406, 498
Eco31I GGTCTC 1 cut(s) 76
Eco47I GGWCC 2 cut(s) 96, 315
Eco53kI GAGCTC 1 cut(s) 404
EcoICRI GAGCTC 1 cut(s) 404
EcoRII CCWGG 3 cut(s) 141, 235, 476
EcoT14I CCWWGG 2 cut(s) 490, 600
EcoT38I GRGCYC 2 cut(s) 406, 498
ErhI CCWWGG 2 cut(s) 490, 600
FaeI CATG 2 cut(s) 329, 604
FaiI YATR 7 cut(s) 225, 327, 357, 438, 483, 507, 602
FaqI GGGAC 1 cut(s) 5
FatI CATG 2 cut(s) 325, 600
Fnu4HI GCNGC 1 cut(s) 660
FokI GGATG 3 cut(s) 153, 385, 712
FriOI GRGCYC 2 cut(s) 406, 498
Fsp4HI GCNGC 1 cut(s) 660
FspBI CTAG 1 cut(s) 35
GlaI GCGC 1 cut(s) 452
GluI GCNGC 1 cut(s) 660
HaeIII GGCC 3 cut(s) 5, 330, 632
HhaI GCGC 1 cut(s) 453
Hin1II CATG 2 cut(s) 329, 604
Hin6I GCGC 1 cut(s) 451
HinP1I GCGC 1 cut(s) 451
HincII GTYRAC 1 cut(s) 552
HindII GTYRAC 1 cut(s) 552
HinfI GANTC 3 cut(s) 42, 122, 596
HphI GGTGA 2 cut(s) 142, 660
Hpy166II GTNNAC 2 cut(s) 177, 552
Hpy188I TCNGA 2 cut(s) 121, 709
Hpy188III TCNNGA 3 cut(s) 73, 243, 424
Hpy8I GTNNAC 2 cut(s) 177, 552
HpyAV CCTTC 2 cut(s) 328, 440
HpyCH4III ACNGT 2 cut(s) 181, 457
HpyCH4V TGCA 4 cut(s) 62, 265, 387, 662
HpyF10VI GCNNNNNNNGC 1 cut(s) 336
HpyF3I CTNAG 4 cut(s) 120, 405, 638, 706
Hsp92II CATG 2 cut(s) 329, 604
HspAI GCGC 1 cut(s) 451
Kzo9I GATC 2 cut(s) 300, 381
LguI GCTCTTC 2 cut(s) 35, 168
LmnI GCTCC 1 cut(s) 401
Lsp1109I GCAGC 1 cut(s) 646
LweI GCATC 1 cut(s) 156
MaeI CTAG 1 cut(s) 35
MalI GATC 2 cut(s) 302, 383
MboI GATC 2 cut(s) 300, 381
MboII GAAGA 8 cut(s) 22, 108, 149, 155, 265, 266, 353, 478
MflI RGATCY 1 cut(s) 300
MhlI GDGCHC 2 cut(s) 406, 498
MlsI TGGCCA 2 cut(s) 5, 330
MluCI AATT 2 cut(s) 213, 349
MluNI TGGCCA 2 cut(s) 5, 330
MlyI GAGTC 2 cut(s) 131, 605
MnlI CCTC 3 cut(s) 136, 434, 647
Mox20I TGGCCA 2 cut(s) 5, 330
MscI TGGCCA 2 cut(s) 5, 330
MseI TTAA 2 cut(s) 609, 733
Msp20I TGGCCA 2 cut(s) 5, 330
MspR9I CCNGG 3 cut(s) 143, 237, 478
Mva1269I GAATGC 1 cut(s) 387
MvaI CCWGG 3 cut(s) 143, 237, 478
MwoI GCNNNNNNNGC 1 cut(s) 336
NcoI CCATGG 1 cut(s) 600
NdeII GATC 2 cut(s) 300, 381
NlaIII CATG 2 cut(s) 329, 604
PciSI GCTCTTC 2 cut(s) 35, 168
PctI GAATGC 1 cut(s) 387
PfeI GAWTC 1 cut(s) 42
PkrI GCNGC 1 cut(s) 661
PleI GAGTC 2 cut(s) 130, 604
PpsI GAGTC 2 cut(s) 130, 604
Psp124BI GAGCTC 1 cut(s) 406
Psp6I CCWGG 3 cut(s) 141, 235, 476
PspGI CCWGG 3 cut(s) 141, 235, 476
PspPI GGNCC 2 cut(s) 96, 315
PstI CTGCAG 1 cut(s) 664
PstNI CAGNNNCTG 1 cut(s) 533
PsuI RGATCY 1 cut(s) 300
RsaI GTAC 1 cut(s) 677
RsaNI GTAC 1 cut(s) 676
SacI GAGCTC 1 cut(s) 406
SapI GCTCTTC 2 cut(s) 35, 168
SaqAI TTAA 2 cut(s) 609, 733
SatI GCNGC 1 cut(s) 660
Sau3AI GATC 2 cut(s) 300, 381
Sau96I GGNCC 2 cut(s) 96, 315
SchI GAGTC 2 cut(s) 131, 605
ScrFI CCNGG 3 cut(s) 143, 237, 478
SduI GDGCHC 2 cut(s) 406, 498
SetI ASST 9 cut(s) 13, 98, 104, 155, 163, 230, 283, 406, 431
SfaNI GCATC 1 cut(s) 156
SfcI CTRYAG 3 cut(s) 355, 522, 660
SinI GGWCC 2 cut(s) 96, 315
SmlI CTYRAG 1 cut(s) 424
SmoI CTYRAG 1 cut(s) 424
Sse9I AATT 2 cut(s) 213, 349
SspI AATATT 1 cut(s) 654
SspMI CTAG 1 cut(s) 35
SstI GAGCTC 1 cut(s) 406
StyD4I CCNGG 3 cut(s) 141, 235, 476
StyI CCWWGG 2 cut(s) 490, 600
TaaI ACNGT 2 cut(s) 181, 457
TaqI TCGA 1 cut(s) 74
TasI AATT 2 cut(s) 213, 349
TfiI GAWTC 1 cut(s) 42
Tru1I TTAA 2 cut(s) 609, 733
Tru9I TTAA 2 cut(s) 609, 733
TscAI CASTG 6 cut(s) 69, 208, 267, 413, 522, 646
TseI GCWGC 1 cut(s) 659
TspDTI ATGAA 5 cut(s) 78, 95, 155, 314, 353
TspRI CASTG 6 cut(s) 69, 208, 267, 413, 522, 646
VpaK11BI GGWCC 2 cut(s) 96, 315
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.