Rorug06G0020400

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0012574
Physical Location & Seq
Reverse (-)
2548631 .. 2578883
30253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0020400.1

Sequence Viewer

Length: 1347 bp
ATGATAATTTCCTCATCGACTGCCATGGCAAAACCAAGCTTCCCTCACATCTTATCCTTATTGCTTCTCATTCTTCTCTTCACATTTTCATCTCTGCCAGGCACATTCGCAGCTCGCAGACTCTCACTCAAAGAAGCCACCATAGACGATCTCCAGCTCGCTTTTAAGCACAACCAACTCACCTCGAGGCAGCTTGTCCAATATTACCATTCCCAAATCCGAAAACTGAACCCGGTTCTCAAAGGAGTCCTAGAAGTAAACCCGGATGCGCTTCACCTTGCCGACAAGGCTGACTACGAGCGCAGGACCAAGGCACCAGTGTCACTACTCTCTAAGTTGCATGGCATTCCTATTCTGGTCAAGGACAACATTGCAACCAAGGATAAGATGAACACCACCGCCGGCTCTTTTGCACTGCTTCGATCTGTCTTGCCTCGTGATGCCGGCGTGGTGGAGAAGTTGAGGAAAGCTGGGGCAATCATTTTGGGAAAGGCTAGCTTGAGCGAGTGGTCACATTTTAGGTCTAATAAAGCACCCTCCGGTTGGAATGCCAGAGGCGGTTATGGCTTGAACCCTTACACATTCTCAGACCCTTGCGGCTCAAGCAGCGGTTCAGCAATATCAGTGGCAGCAAATCTGGCAGCGGTGTCATTAGGTACAGAGACAGATGGTTCAATCTTATGTCCATCAGGTTATAACTCAGTTGTGGGCATCAAACCAACAATCGGTCTTACTAGTCGAGCAGGGGTAGTCCCAATTTCTCTAAGACAGGATACTGTCGGGCCAATTTGTAGGACAGTAGCAGATGCTGCTTATGTTCTTGATGCCATAGCAGGCATCGACCACAATGACATTGCAACGATTGAGACATCAAAGTACATTCCCAAAGGTGGTTATGCACAGTTTCTCAGGCGCGACGGACTTAGAGGAAAAAGAATAGGGATATTGAGAGCCTTCTATGACTTTGGCAATGACACTTCCTTGGCTCAAACTTTTGAGAAACATCTCAACACTCTGAGGAAAGGAGGTGCTGTTTTGGTAGACAATTTGGAAATTGCTAATTTGACTGAAATCTATTCCTCAAACGATGAATATAATGCATTGTATATTGAGTTCAAAATATCCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCAGTGCGATCCTTGGCAGATGCTATAGCCTTTAACAACAAACATCAGAAACTGGAAAAGATCAAGGAGTATGGGCAAGACCTATTTTTAGCAGCTGAAGCAACAAATGGTTTTGGAAAAACAGAGATGCCAGCATTGTGGAATCTTGCAAGGTGGACAAGATATGGTCTTGTGAAATTAATGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ DnaJ-X

Protein Analysis

448

Amino Acids

48.82

Weight (kDa)

9.44

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 65 - 407 2.3e-68 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 696
AbsI CCTCGAGG 1 cut(s) 184
AccB1I GGYRCC 1 cut(s) 313
AccI GTMKAC 1 cut(s) 1041
AccII CGCG 1 cut(s) 915
AciI CCGC 5 cut(s) 399, 558, 597, 609, 644
AclWI GGATC 1 cut(s) 1161
AcuI CTGAAG 1 cut(s) 1275
AfaI GTAC 2 cut(s) 658, 878
AfiI CCNNNNNNNGG 3 cut(s) 543, 725, 890
AgsI TTSAA 4 cut(s) 571, 675, 1117, 1141
AhlI ACTAGT 1 cut(s) 734
AjnI CCWGG 1 cut(s) 97
AluBI AGCT 7 cut(s) 39, 113, 157, 193, 470, 498, 1253
AluI AGCT 7 cut(s) 39, 113, 157, 193, 470, 498, 1253
Alw26I GTCTC 2 cut(s) 656, 860
AlwI GGATC 1 cut(s) 1161
AlwNI CAGNNNCTG 2 cut(s) 809, 1210
Ama87I CYCGRG 1 cut(s) 184
AoxI GGCC 1 cut(s) 782
ApeKI GCWGC 7 cut(s) 110, 190, 606, 629, 641, 809, 1250
AseI ATTAAT 1 cut(s) 1337
AspLEI GCGC 3 cut(s) 271, 303, 915
AspS9I GGNCC 2 cut(s) 306, 782
AsuC2I CCSGG 2 cut(s) 233, 263
AsuHPI GGTGA 2 cut(s) 172, 266
AsuNHI GCTAGC 1 cut(s) 494
AvaI CYCGRG 1 cut(s) 184
AvaII GGWCC 1 cut(s) 306
BanI GGYRCC 1 cut(s) 313
BauI CACGAG 1 cut(s) 435
BbvI GCAGC 7 cut(s) 122, 202, 618, 641, 653, 796, 1262
BccI CCATC 2 cut(s) 662, 694
BciT130I CCWGG 1 cut(s) 99
BciVI GTATCC 1 cut(s) 766
BcnI CCSGG 2 cut(s) 233, 263
BcoDI GTCTC 2 cut(s) 656, 860
BcuI ACTAGT 1 cut(s) 734
BfaI CTAG 3 cut(s) 251, 495, 735
BfmI CTRYAG 1 cut(s) 1182
BfuI GTATCC 1 cut(s) 766
BglI GCCNNNNNGGC 1 cut(s) 287
BisI GCNGC 8 cut(s) 111, 191, 598, 607, 630, 642, 810, 1251
BlsI GCNGC 8 cut(s) 112, 192, 599, 608, 631, 643, 811, 1252
Bme1390I CCNGG 3 cut(s) 99, 233, 263
Bme18I GGWCC 1 cut(s) 306
BmeT110I CYCGRG 1 cut(s) 184
BmgT120I GGNCC 2 cut(s) 306, 782
BmiI GGNNCC 1 cut(s) 315
BmrFI CCNGG 3 cut(s) 99, 233, 263
BmrI ACTGGG 1 cut(s) 1154
BmsI GCATC 8 cut(s) 256, 430, 720, 796, 814, 846, 1168, 1275
BmtI GCTAGC 1 cut(s) 498
BmuI ACTGGG 1 cut(s) 1154
BpmI CTGGAG 1 cut(s) 137
BpuEI CTTGAG 2 cut(s) 520, 586
BpuMI CCSGG 2 cut(s) 233, 263
BsaJI CCNNGG 5 cut(s) 24, 309, 378, 981, 1170
BsaWI WCCGGW 1 cut(s) 539
BsaXI ACNNNNNCTCC 2 cut(s) 1017, 1047
Bsc4I CCNNNNNNNGG 3 cut(s) 543, 725, 890
Bse118I RCCGGY 2 cut(s) 401, 443
Bse1I ACTGG 3 cut(s) 317, 1160, 1215
Bse3DI GCAATG 3 cut(s) 369, 852, 976
BseBI CCWGG 1 cut(s) 99
BseDI CCNNGG 5 cut(s) 24, 309, 378, 981, 1170
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 3 cut(s) 543, 725, 890
BseMI GCAATG 3 cut(s) 369, 852, 976
BseMII CTCAG 4 cut(s) 600, 714, 922, 1007
BseNI ACTGG 3 cut(s) 317, 1160, 1215
BseXI GCAGC 7 cut(s) 122, 202, 618, 641, 653, 796, 1262
BseYI CCCAGC 1 cut(s) 470
Bsh1236I CGCG 1 cut(s) 915
BshFI GGCC 1 cut(s) 784
BshNI GGYRCC 1 cut(s) 313
BsiHKCI CYCGRG 1 cut(s) 184
BsiSI CCGG 5 cut(s) 233, 263, 402, 444, 540
BslFI GGGAC 1 cut(s) 737
BslI CCNNNNNNNGG 3 cut(s) 543, 725, 890
BsmAI GTCTC 2 cut(s) 656, 860
BsmFI GGGAC 1 cut(s) 737
BsmI GAATGC 2 cut(s) 345, 553
BsnI GGCC 1 cut(s) 784
BsoBI CYCGRG 1 cut(s) 184
Bsp143I GATC 4 cut(s) 148, 422, 1166, 1218
Bsp19I CCATGG 1 cut(s) 24
BspACI CCGC 5 cut(s) 399, 558, 597, 609, 644
BspANI GGCC 1 cut(s) 784
BspCNI CTCAG 4 cut(s) 599, 713, 921, 1008
BspFNI CGCG 1 cut(s) 915
BspLI GGNNCC 1 cut(s) 315
BspOI GCTAGC 1 cut(s) 498
BspPI GGATC 1 cut(s) 1161
BspT107I GGYRCC 1 cut(s) 313
BsrDI GCAATG 3 cut(s) 369, 852, 976
BsrFI RCCGGY 2 cut(s) 401, 443
BsrI ACTGG 3 cut(s) 317, 1160, 1215
BssAI RCCGGY 2 cut(s) 401, 443
BssECI CCNNGG 5 cut(s) 24, 309, 378, 981, 1170
BssMI GATC 4 cut(s) 148, 422, 1166, 1218
BssSI CACGAG 1 cut(s) 435
BssT1I CCWWGG 5 cut(s) 24, 309, 378, 981, 1170
Bst2BI CACGAG 1 cut(s) 435
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 3 cut(s) 778, 799, 903
Bst6I CTCTTC 1 cut(s) 83
BstAPI GCANNNNNTGC 1 cut(s) 809
BstC8I GCNNGC 7 cut(s) 115, 159, 403, 445, 496, 835, 1290
BstDEI CTNAG 7 cut(s) 333, 586, 700, 764, 908, 923, 1016
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 1 cut(s) 271
BstFNI CGCG 1 cut(s) 915
BstHHI GCGC 3 cut(s) 271, 303, 915
BstKTI GATC 4 cut(s) 151, 425, 1169, 1221
BstMAI GTCTC 2 cut(s) 656, 860
BstMBI GATC 4 cut(s) 148, 422, 1166, 1218
BstMWI GCNNNNNNNGC 7 cut(s) 287, 564, 603, 606, 638, 809, 1256
BstNI CCWGG 1 cut(s) 99
BstSCI CCNGG 3 cut(s) 97, 231, 261
BstSFI CTRYAG 1 cut(s) 1182
BstUI CGCG 1 cut(s) 915
BstV1I GCAGC 7 cut(s) 122, 202, 618, 641, 653, 796, 1262
BstXI CCANNNNNNTGG 1 cut(s) 1296
BsuI GTATCC 1 cut(s) 766
BsuRI GGCC 1 cut(s) 784
BtgI CCRYGG 1 cut(s) 24
BtsCI GGATG 1 cut(s) 271
BtsI GCAGTG 1 cut(s) 413
BtsIMutI CAGTG 4 cut(s) 324, 413, 630, 1167
Cac8I GCNNGC 7 cut(s) 115, 159, 403, 445, 496, 835, 1290
CaiI CAGNNNCTG 2 cut(s) 809, 1210
CfoI GCGC 3 cut(s) 271, 303, 915
Cfr10I RCCGGY 2 cut(s) 401, 443
Cfr13I GGNCC 2 cut(s) 306, 782
Csp6I GTAC 2 cut(s) 657, 877
CspCI CAANNNNNGTGG 2 cut(s) 606, 641
CviAII CATG 2 cut(s) 25, 341
CviQI GTAC 2 cut(s) 657, 877
DdeI CTNAG 7 cut(s) 333, 586, 700, 764, 908, 923, 1016
DpnI GATC 4 cut(s) 150, 424, 1168, 1220
DpnII GATC 4 cut(s) 148, 422, 1166, 1218
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Eco130I CCWWGG 5 cut(s) 24, 309, 378, 981, 1170
Eco47I GGWCC 1 cut(s) 306
Eco57I CTGAAG 1 cut(s) 1275
Eco88I CYCGRG 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 97
EcoT14I CCWWGG 5 cut(s) 24, 309, 378, 981, 1170
EcoT22I ATGCAT 2 cut(s) 1102, 1135
ErhI CCWWGG 5 cut(s) 24, 309, 378, 981, 1170
FaeI CATG 2 cut(s) 28, 344
FalI AAGNNNNNCTT 2 cut(s) 482, 514
FaqI GGGAC 1 cut(s) 737
FatI CATG 2 cut(s) 24, 340
FblI GTMKAC 1 cut(s) 1041
Fnu4HI GCNGC 8 cut(s) 111, 191, 598, 607, 630, 642, 810, 1251
FokI GGATG 1 cut(s) 278
Fsp4HI GCNGC 8 cut(s) 111, 191, 598, 607, 630, 642, 810, 1251
FspBI CTAG 3 cut(s) 251, 495, 735
GlaI GCGC 3 cut(s) 270, 302, 914
GluI GCNGC 8 cut(s) 111, 191, 598, 607, 630, 642, 810, 1251
GsaI CCCAGC 1 cut(s) 474
GsuI CTGGAG 1 cut(s) 137
HaeIII GGCC 1 cut(s) 784
HapII CCGG 5 cut(s) 233, 263, 402, 444, 540
HhaI GCGC 3 cut(s) 271, 303, 915
Hin1II CATG 2 cut(s) 28, 344
Hin6I GCGC 3 cut(s) 269, 301, 913
HinP1I GCGC 3 cut(s) 269, 301, 913
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 3 cut(s) 120, 246, 1300
HpaII CCGG 5 cut(s) 233, 263, 402, 444, 540
HphI GGTGA 2 cut(s) 172, 266
Hpy166II GTNNAC 3 cut(s) 259, 1042, 1314
Hpy188I TCNGA 4 cut(s) 221, 589, 1017, 1206
Hpy188III TCNNGA 2 cut(s) 437, 821
Hpy8I GTNNAC 3 cut(s) 259, 1042, 1314
Hpy99I CGWCG 1 cut(s) 920
HpyAV CCTTC 2 cut(s) 964, 1135
HpyCH4III ACNGT 3 cut(s) 778, 799, 903
HpyCH4V TGCA 8 cut(s) 340, 374, 413, 857, 899, 1100, 1133, 1307
HpyF10VI GCNNNNNNNGC 7 cut(s) 287, 564, 603, 606, 638, 809, 1256
HpyF3I CTNAG 7 cut(s) 333, 586, 700, 764, 908, 923, 1016
Hsp92II CATG 2 cut(s) 28, 344
HspAI GCGC 3 cut(s) 269, 301, 913
KroI GCCGGC 2 cut(s) 401, 443
KroNI GCCGGC 2 cut(s) 403, 445
Kzo9I GATC 4 cut(s) 148, 422, 1166, 1218
Lsp1109I GCAGC 7 cut(s) 122, 202, 618, 641, 653, 796, 1262
LweI GCATC 8 cut(s) 256, 430, 720, 796, 814, 846, 1168, 1275
MaeI CTAG 3 cut(s) 251, 495, 735
MaeIII GTNAC 2 cut(s) 321, 510
MalI GATC 4 cut(s) 150, 424, 1168, 1220
MboI GATC 4 cut(s) 148, 422, 1166, 1218
MboII GAAGA 2 cut(s) 65, 70
MluCI AATT 7 cut(s) 6, 756, 786, 1045, 1053, 1060, 1334
MlyI GAGTC 2 cut(s) 114, 255
MmeI TCCRAC 1 cut(s) 524
Mph1103I ATGCAT 2 cut(s) 1102, 1135
MroNI GCCGGC 2 cut(s) 401, 443
MseI TTAA 4 cut(s) 165, 1127, 1191, 1337
MspA1I CMGCKG 3 cut(s) 609, 644, 1253
MspI CCGG 5 cut(s) 233, 263, 402, 444, 540
MspR9I CCNGG 3 cut(s) 99, 233, 263
Mva1269I GAATGC 2 cut(s) 345, 553
MvaI CCWGG 1 cut(s) 99
MvnI CGCG 1 cut(s) 915
MwoI GCNNNNNNNGC 7 cut(s) 287, 564, 603, 606, 638, 809, 1256
NaeI GCCGGC 2 cut(s) 403, 445
NciI CCSGG 2 cut(s) 233, 263
NcoI CCATGG 1 cut(s) 24
NdeII GATC 4 cut(s) 148, 422, 1166, 1218
NgoMIV GCCGGC 2 cut(s) 401, 443
NheI GCTAGC 1 cut(s) 494
NlaIII CATG 2 cut(s) 28, 344
NlaIV GGNNCC 1 cut(s) 315
NmuCI GTSAC 2 cut(s) 321, 510
NsiI ATGCAT 2 cut(s) 1102, 1135
PaeR7I CTCGAG 1 cut(s) 184
PctI GAATGC 2 cut(s) 345, 553
PdiI GCCGGC 2 cut(s) 403, 445
PfeI GAWTC 1 cut(s) 1300
PkrI GCNGC 8 cut(s) 112, 192, 599, 608, 631, 643, 811, 1252
PleI GAGTC 2 cut(s) 114, 254
PpsI GAGTC 2 cut(s) 114, 254
PshBI ATTAAT 1 cut(s) 1337
PsiI TTATAA 1 cut(s) 696
Psp6I CCWGG 1 cut(s) 97
PspFI CCCAGC 1 cut(s) 470
PspGI CCWGG 1 cut(s) 97
PspN4I GGNNCC 1 cut(s) 315
PspPI GGNCC 2 cut(s) 306, 782
PspXI VCTCGAGB 1 cut(s) 184
PsrI GAACNNNNNNTAC 2 cut(s) 1097, 1129
PstNI CAGNNNCTG 2 cut(s) 809, 1210
PvuII CAGCTG 1 cut(s) 1253
RsaI GTAC 2 cut(s) 658, 878
RsaNI GTAC 2 cut(s) 657, 877
SaqAI TTAA 4 cut(s) 165, 1127, 1191, 1337
SatI GCNGC 8 cut(s) 111, 191, 598, 607, 630, 642, 810, 1251
Sau3AI GATC 4 cut(s) 148, 422, 1166, 1218
Sau96I GGNCC 2 cut(s) 306, 782
SchI GAGTC 2 cut(s) 114, 255
ScrFI CCNGG 3 cut(s) 99, 233, 263
SfaNI GCATC 8 cut(s) 256, 430, 720, 796, 814, 846, 1168, 1275
SfcI CTRYAG 1 cut(s) 1182
Sfr274I CTCGAG 1 cut(s) 184
SinI GGWCC 1 cut(s) 306
SlaI CTCGAG 1 cut(s) 184
SmlI CTYRAG 3 cut(s) 184, 499, 601
SmoI CTYRAG 3 cut(s) 184, 499, 601
SpeI ACTAGT 1 cut(s) 734
Sse9I AATT 7 cut(s) 6, 756, 786, 1045, 1053, 1060, 1334
SsiI CCGC 5 cut(s) 399, 558, 597, 609, 644
SspI AATATT 1 cut(s) 203
SspMI CTAG 3 cut(s) 251, 495, 735
StyD4I CCNGG 3 cut(s) 97, 231, 261
StyI CCWWGG 5 cut(s) 24, 309, 378, 981, 1170
TaaI ACNGT 3 cut(s) 778, 799, 903
TaqI TCGA 5 cut(s) 17, 185, 421, 739, 840
TaqII GACCGA 1 cut(s) 716
TasI AATT 7 cut(s) 6, 756, 786, 1045, 1053, 1060, 1334
TatI WGTACW 1 cut(s) 876
TauI GCSGC 1 cut(s) 600
TfiI GAWTC 1 cut(s) 1300
Tru1I TTAA 4 cut(s) 165, 1127, 1191, 1337
Tru9I TTAA 4 cut(s) 165, 1127, 1191, 1337
TscAI CASTG 4 cut(s) 324, 420, 630, 1167
TseFI GTSAC 2 cut(s) 321, 510
TseI GCWGC 7 cut(s) 110, 190, 606, 629, 641, 809, 1250
Tsp45I GTSAC 2 cut(s) 321, 510
TspDTI ATGAA 3 cut(s) 78, 404, 1104
TspGWI ACGGA 1 cut(s) 933
TspRI CASTG 4 cut(s) 324, 420, 630, 1167
VpaK11BI GGWCC 1 cut(s) 306
VspI ATTAAT 1 cut(s) 1337
XhoI CTCGAG 1 cut(s) 184
XmiI GTMKAC 1 cut(s) 1041
XspI CTAG 3 cut(s) 251, 495, 735
Zsp2I ATGCAT 2 cut(s) 1102, 1135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.