Rorug06G0020500

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0012574
Physical Location & Seq
Reverse (-)
2586057 .. 2588968
2912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0020500.1

Sequence Viewer

Length: 1557 bp
ATGATAATTTCCTCATCCACTGCCATGGCGAAATTCCCTCACATCTCATGCTTATTGCTTCTCTTCACATTGTCATCTCTTCCAGAGACATTCGCAGCCCGCAGACTCTCACTCAAAGAAGCCACCATAGACGATCTCCAGCTCGCTTTCAAGCACAACCAACTCACCTCGAGGCAACTTGTTCAATATTACCTTGCCCAAATCCGAAAACTGAACCCGGTTCTCAAAGGAGTCCTAGAAGTCAATCCAGATGCGCTTCACCTTGCCGACAAGGCTGACTACGAGCGCAGGACCAAGGCACCAGTGTCACTACTCTCTAAGTTGCATGGCATTCCTATTCTGGTCAAGGACAACATTGCAACCAAGGATAAGATGAACACCACCGCCGGCTCCTTTGCGCTGTTTGGATCTGTCGTGCCTCGTGATGCCGGCGTGGTGGAGAAGTTGCGGAAAGCAGGGGCTATCATTTTCGGAAAGGCTAGCTTGAGCGAGTGGTCACAGTTTAGGTCTACTAAAACCCCCTCTGGTTGGAGCGCCAGAGGCGGTTATGGCTTGAACCCTTACACATTCTCAGACCCTTGCGGCTCAAGCAGCGGTTCAGCAATATCAGTGGCAGCAAATCTGGCAGCGGTGTCATTAGGTACCGAGACAAGTGGTTCAATCTTATGTCCATCAAGTTTGAACTCAGTTGTGGGCCTCAAACCAACAGTCGGTCTTACTAGTCGAGCAGGGGTTGTCCCAATTTCTCTAAGACAGGATACTGTTGGGCCAATTTGTAGGACAGTAGCAGATGCTGCTTATGTTCTTGATGCCATAGCAGGGATCGACCACAATGACATTGCAACAATTGAGACATCAAAGTACATTCCCAAAGGTGGTTATGCACAGTTTCTCAGGCGCGACGGACTTAGTGGAAAAAGAATAGGGATATTGGGAGCCTTCCATGACTTTGGCAATGACACTTCCTTAGCTCAAACTTTTGAGAAACATCTCAACACTCTGAGGAAAGGAGGAGCTGTTTTGGTAGACAATTTGAAAATTGCTAATTTGACTGAAATCTATTCTAGAAGCGATGAATATGCTGCATTGTATATTGAGTTCAAAATAACCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCGGTGCGATCCTTGGCAGATGTTATAGCCTTCAACAACAAACACCAGAAACTGGAAAAAGTCAAGGAGTATGGGCAAGACCGATTTTTAGCAGCTGAAGCAACAAATGGGTTTGGAAAAACAGAGAAGCTGGCATTGTTGAATCTTGCAAGGTGGACAAGAAATGGTCTTGTGAAATTGGTGACAGAGAAGAAGCTAGATGCTGTGGTGTCTCCTTACGCAAGTTTGTCACGTGTACTTGCAATTGGGGGAGCCCCAGGTATAATAGTTCCAGCTGGATACGAAAACAATGGGGAACCAGCTGGTATATGCTTTGGTGGACTTCGGGGTTCAGAGCCAAAGCTAATCGAGATTGCATATGCTTTTGAGCAAGCCACTAAGATTAGGAAGCCTCCTTCATTCAAAGGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ DnaJ-X

Protein Analysis

518

Amino Acids

55.71

Weight (kDa)

9.48

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 60 - 456 1.3e-72 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 169
Acc65I GGTACC 1 cut(s) 641
AccB1I GGYRCC 2 cut(s) 298, 641
AccB7I CCANNNNNTGG 1 cut(s) 1195
AccI GTMKAC 2 cut(s) 509, 1026
AccII CGCG 1 cut(s) 900
AciI CCGC 7 cut(s) 100, 384, 448, 543, 582, 594, 629
AclWI GGATC 3 cut(s) 415, 830, 1146
AcsI RAATTY 1 cut(s) 32
AcuI CTGAAG 1 cut(s) 1260
AcvI CACGTG 1 cut(s) 1376
AfaI GTAC 3 cut(s) 643, 863, 1380
AfiI CCNNNNNNNGG 5 cut(s) 528, 710, 819, 875, 1195
AflIII ACRYGT 1 cut(s) 1375
AhlI ACTAGT 1 cut(s) 719
AjnI CCWGG 1 cut(s) 1399
Alw26I GTCTC 4 cut(s) 80, 641, 845, 1359
AlwI GGATC 3 cut(s) 415, 830, 1146
AlwNI CAGNNNCTG 2 cut(s) 794, 1195
Ama87I CYCGRG 1 cut(s) 169
AoxI GGCC 2 cut(s) 694, 767
ApeKI GCWGC 7 cut(s) 95, 591, 614, 626, 794, 1082, 1235
ApoI RAATTY 1 cut(s) 32
ArsI GACNNNNNNTTYG 2 cut(s) 693, 725
Asp718I GGTACC 1 cut(s) 641
AspLEI GCGC 5 cut(s) 256, 288, 400, 536, 900
AspS9I GGNCC 3 cut(s) 291, 694, 767
AsuC2I CCSGG 2 cut(s) 218, 1145
AsuHPI GGTGA 3 cut(s) 157, 251, 1336
AsuNHI GCTAGC 1 cut(s) 479
AvaI CYCGRG 1 cut(s) 169
AvaII GGWCC 1 cut(s) 291
BanI GGYRCC 2 cut(s) 298, 641
BanII GRGCYC 1 cut(s) 1399
BauI CACGAG 1 cut(s) 420
BbrPI CACGTG 1 cut(s) 1376
BbvI GCAGC 7 cut(s) 107, 603, 626, 638, 781, 1069, 1247
BccI CCATC 1 cut(s) 679
BcgI CGANNNNNNTGC 2 cut(s) 1061, 1095
BciT130I CCWGG 1 cut(s) 1401
BciVI GTATCC 2 cut(s) 751, 1415
BcnI CCSGG 2 cut(s) 218, 1145
BcoDI GTCTC 4 cut(s) 80, 641, 845, 1359
BcuI ACTAGT 1 cut(s) 719
BfaI CTAG 6 cut(s) 236, 480, 720, 1065, 1340, 1555
BfoI RGCGCY 1 cut(s) 537
BfuI GTATCC 2 cut(s) 751, 1415
BglI GCCNNNNNGGC 1 cut(s) 272
BisI GCNGC 8 cut(s) 96, 583, 592, 615, 627, 795, 1083, 1236
BlsI GCNGC 8 cut(s) 97, 584, 593, 616, 628, 796, 1084, 1237
Bme1390I CCNGG 3 cut(s) 218, 1145, 1401
Bme18I GGWCC 1 cut(s) 291
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 3 cut(s) 291, 694, 767
BmiI GGNNCC 6 cut(s) 300, 391, 643, 937, 1396, 1440
BmrFI CCNGG 3 cut(s) 218, 1145, 1401
BmsI GCATC 5 cut(s) 241, 415, 781, 799, 1333
BmtI GCTAGC 1 cut(s) 483
BpmI CTGGAG 1 cut(s) 122
Bpu10I CCTNAGC 1 cut(s) 967
BpuEI CTTGAG 2 cut(s) 505, 571
BpuMI CCSGG 2 cut(s) 218, 1145
BsaAI YACGTR 1 cut(s) 1376
BsaJI CCNNGG 6 cut(s) 24, 294, 363, 1143, 1155, 1399
BsaXI ACNNNNNCTCC 2 cut(s) 1002, 1032
Bsc4I CCNNNNNNNGG 5 cut(s) 528, 710, 819, 875, 1195
Bse118I RCCGGY 2 cut(s) 386, 428
Bse1I ACTGG 2 cut(s) 302, 1200
Bse3DI GCAATG 3 cut(s) 354, 837, 961
BseBI CCWGG 1 cut(s) 1401
BseDI CCNNGG 6 cut(s) 24, 294, 363, 1143, 1155, 1399
BseGI GGATG 1 cut(s) 14
BseLI CCNNNNNNNGG 5 cut(s) 528, 710, 819, 875, 1195
BseMI GCAATG 3 cut(s) 354, 837, 961
BseMII CTCAG 4 cut(s) 585, 699, 907, 992
BseNI ACTGG 2 cut(s) 302, 1200
BseRI GAGGAG 1 cut(s) 1026
BseXI GCAGC 7 cut(s) 107, 603, 626, 638, 781, 1069, 1247
Bsh1236I CGCG 1 cut(s) 900
BshFI GGCC 2 cut(s) 696, 769
BshNI GGYRCC 2 cut(s) 298, 641
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 4 cut(s) 218, 387, 429, 1145
BslFI GGGAC 1 cut(s) 722
BslI CCNNNNNNNGG 5 cut(s) 528, 710, 819, 875, 1195
BsmAI GTCTC 4 cut(s) 80, 641, 845, 1359
BsmFI GGGAC 1 cut(s) 722
BsmI GAATGC 1 cut(s) 330
BsnI GGCC 2 cut(s) 696, 769
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 1399
Bsp143I GATC 4 cut(s) 133, 407, 822, 1151
Bsp19I CCATGG 1 cut(s) 24
BspACI CCGC 7 cut(s) 100, 384, 448, 543, 582, 594, 629
BspANI GGCC 2 cut(s) 696, 769
BspCNI CTCAG 4 cut(s) 584, 698, 906, 993
BspFNI CGCG 1 cut(s) 900
BspLI GGNNCC 6 cut(s) 300, 391, 643, 937, 1396, 1440
BspOI GCTAGC 1 cut(s) 483
BspPI GGATC 3 cut(s) 415, 830, 1146
BspT107I GGYRCC 2 cut(s) 298, 641
BsrDI GCAATG 3 cut(s) 354, 837, 961
BsrFI RCCGGY 2 cut(s) 386, 428
BsrI ACTGG 2 cut(s) 302, 1200
BssAI RCCGGY 2 cut(s) 386, 428
BssECI CCNNGG 6 cut(s) 24, 294, 363, 1143, 1155, 1399
BssMI GATC 4 cut(s) 133, 407, 822, 1151
BssSI CACGAG 1 cut(s) 420
BssT1I CCWWGG 4 cut(s) 24, 294, 363, 1155
Bst2BI CACGAG 1 cut(s) 420
Bst2UI CCWGG 1 cut(s) 1401
Bst4CI ACNGT 5 cut(s) 501, 709, 763, 784, 888
Bst6I CTCTTC 2 cut(s) 68, 84
BstAPI GCANNNNNTGC 1 cut(s) 794
BstBAI YACGTR 1 cut(s) 1376
BstC8I GCNNGC 7 cut(s) 100, 144, 388, 430, 481, 1275, 1515
BstDEI CTNAG 9 cut(s) 318, 571, 685, 749, 893, 908, 967, 1001, 1521
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 1 cut(s) 14
BstFNI CGCG 1 cut(s) 900
BstH2I RGCGCY 1 cut(s) 537
BstHHI GCGC 5 cut(s) 256, 288, 400, 536, 900
BstKTI GATC 4 cut(s) 136, 410, 825, 1154
BstMAI GTCTC 4 cut(s) 80, 641, 845, 1359
BstMBI GATC 4 cut(s) 133, 407, 822, 1151
BstMWI GCNNNNNNNGC 8 cut(s) 272, 540, 549, 588, 591, 623, 794, 1241
BstNI CCWGG 1 cut(s) 1401
BstSCI CCNGG 3 cut(s) 216, 1143, 1399
BstUI CGCG 1 cut(s) 900
BstV1I GCAGC 7 cut(s) 107, 603, 626, 638, 781, 1069, 1247
BstX2I RGATCY 1 cut(s) 407
BstXI CCANNNNNNTGG 2 cut(s) 25, 950
BstYI RGATCY 1 cut(s) 407
BsuI GTATCC 2 cut(s) 751, 1415
BsuRI GGCC 2 cut(s) 696, 769
BtgI CCRYGG 1 cut(s) 24
BtgZI GCGATG 1 cut(s) 1086
BtsCI GGATG 1 cut(s) 14
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 3 cut(s) 18, 309, 615
Cac8I GCNNGC 7 cut(s) 100, 144, 388, 430, 481, 1275, 1515
CaiI CAGNNNCTG 2 cut(s) 794, 1195
CfoI GCGC 5 cut(s) 256, 288, 400, 536, 900
Cfr10I RCCGGY 2 cut(s) 386, 428
Cfr13I GGNCC 3 cut(s) 291, 694, 767
Csp6I GTAC 3 cut(s) 642, 862, 1379
CspCI CAANNNNNGTGG 2 cut(s) 591, 626
CviAII CATG 4 cut(s) 25, 48, 326, 944
CviQI GTAC 3 cut(s) 642, 862, 1379
DdeI CTNAG 9 cut(s) 318, 571, 685, 749, 893, 908, 967, 1001, 1521
DpnI GATC 4 cut(s) 135, 409, 824, 1153
DpnII GATC 4 cut(s) 133, 407, 822, 1151
Eam1104I CTCTTC 2 cut(s) 68, 84
EarI CTCTTC 2 cut(s) 68, 84
Eco130I CCWWGG 4 cut(s) 24, 294, 363, 1155
Eco24I GRGCYC 1 cut(s) 1399
Eco47I GGWCC 1 cut(s) 291
Eco57I CTGAAG 1 cut(s) 1260
Eco72I CACGTG 1 cut(s) 1376
Eco88I CYCGRG 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 1399
EcoT14I CCWWGG 4 cut(s) 24, 294, 363, 1155
EcoT22I ATGCAT 1 cut(s) 1120
EcoT38I GRGCYC 1 cut(s) 1399
ErhI CCWWGG 4 cut(s) 24, 294, 363, 1155
FaeI CATG 4 cut(s) 28, 51, 329, 947
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FaqI GGGAC 1 cut(s) 722
FatI CATG 4 cut(s) 24, 47, 325, 943
FauI CCCGC 1 cut(s) 107
FauNDI CATATG 1 cut(s) 1501
FblI GTMKAC 2 cut(s) 509, 1026
Fnu4HI GCNGC 8 cut(s) 96, 583, 592, 615, 627, 795, 1083, 1236
FriOI GRGCYC 1 cut(s) 1399
Fsp4HI GCNGC 8 cut(s) 96, 583, 592, 615, 627, 795, 1083, 1236
FspBI CTAG 6 cut(s) 236, 480, 720, 1065, 1340, 1555
GlaI GCGC 5 cut(s) 255, 287, 399, 535, 899
GluI GCNGC 8 cut(s) 96, 583, 592, 615, 627, 795, 1083, 1236
GsuI CTGGAG 1 cut(s) 122
HaeII RGCGCY 1 cut(s) 537
HaeIII GGCC 2 cut(s) 696, 769
HapII CCGG 4 cut(s) 218, 387, 429, 1145
HhaI GCGC 5 cut(s) 256, 288, 400, 536, 900
Hin1II CATG 4 cut(s) 28, 51, 329, 947
Hin6I GCGC 5 cut(s) 254, 286, 398, 534, 898
HinP1I GCGC 5 cut(s) 254, 286, 398, 534, 898
HinfI GANTC 3 cut(s) 105, 231, 1285
HpaII CCGG 4 cut(s) 218, 387, 429, 1145
HphI GGTGA 3 cut(s) 157, 251, 1336
Hpy166II GTNNAC 5 cut(s) 510, 1027, 1299, 1379, 1463
Hpy188I TCNGA 5 cut(s) 206, 473, 574, 1002, 1477
Hpy188III TCNNGA 6 cut(s) 83, 248, 422, 806, 1065, 1492
Hpy8I GTNNAC 5 cut(s) 510, 1027, 1299, 1379, 1463
Hpy99I CGWCG 1 cut(s) 905
HpyAV CCTTC 4 cut(s) 949, 1120, 1183, 1548
HpyCH4III ACNGT 5 cut(s) 501, 709, 763, 784, 888
HpyCH4IV ACGT 1 cut(s) 1375
HpyCH4V TGCA 9 cut(s) 325, 359, 842, 884, 1085, 1118, 1292, 1385, 1499
HpyF10VI GCNNNNNNNGC 8 cut(s) 272, 540, 549, 588, 591, 623, 794, 1241
HpyF3I CTNAG 9 cut(s) 318, 571, 685, 749, 893, 908, 967, 1001, 1521
HpySE526I ACGT 1 cut(s) 1375
Hsp92II CATG 4 cut(s) 28, 51, 329, 947
HspAI GCGC 5 cut(s) 254, 286, 398, 534, 898
KpnI GGTACC 1 cut(s) 645
KroI GCCGGC 2 cut(s) 386, 428
KroNI GCCGGC 2 cut(s) 388, 430
Kzo9I GATC 4 cut(s) 133, 407, 822, 1151
LmnI GCTCC 5 cut(s) 395, 531, 935, 1013, 1394
Lsp1109I GCAGC 7 cut(s) 107, 603, 626, 638, 781, 1069, 1247
LweI GCATC 5 cut(s) 241, 415, 781, 799, 1333
MaeI CTAG 6 cut(s) 236, 480, 720, 1065, 1340, 1555
MaeII ACGT 1 cut(s) 1375
MaeIII GTNAC 4 cut(s) 306, 495, 1324, 1371
MalI GATC 4 cut(s) 135, 409, 824, 1153
MboI GATC 4 cut(s) 133, 407, 822, 1151
MboII GAAGA 3 cut(s) 55, 71, 1345
MfeI CAATTG 2 cut(s) 846, 1386
MflI RGATCY 1 cut(s) 407
MhlI GDGCHC 1 cut(s) 1399
MlyI GAGTC 2 cut(s) 99, 240
MmeI TCCRAC 1 cut(s) 509
Mph1103I ATGCAT 1 cut(s) 1120
MroNI GCCGGC 2 cut(s) 386, 428
MseI TTAA 1 cut(s) 1112
MslI CAYNNNNRTG 1 cut(s) 23
MspA1I CMGCKG 5 cut(s) 594, 629, 1238, 1418, 1445
MspI CCGG 4 cut(s) 218, 387, 429, 1145
MspR9I CCNGG 3 cut(s) 218, 1145, 1401
MunI CAATTG 2 cut(s) 846, 1386
Mva1269I GAATGC 1 cut(s) 330
MvaI CCWGG 1 cut(s) 1401
MvnI CGCG 1 cut(s) 900
MwoI GCNNNNNNNGC 8 cut(s) 272, 540, 549, 588, 591, 623, 794, 1241
NaeI GCCGGC 2 cut(s) 388, 430
NciI CCSGG 2 cut(s) 218, 1145
NcoI CCATGG 1 cut(s) 24
NdeI CATATG 1 cut(s) 1501
NdeII GATC 4 cut(s) 133, 407, 822, 1151
NgoMIV GCCGGC 2 cut(s) 386, 428
NheI GCTAGC 1 cut(s) 479
NlaIII CATG 4 cut(s) 28, 51, 329, 947
NlaIV GGNNCC 6 cut(s) 300, 391, 643, 937, 1396, 1440
NmuCI GTSAC 4 cut(s) 306, 495, 1324, 1371
NsiI ATGCAT 1 cut(s) 1120
PaeR7I CTCGAG 1 cut(s) 169
PctI GAATGC 1 cut(s) 330
PdiI GCCGGC 2 cut(s) 388, 430
PfeI GAWTC 1 cut(s) 1285
PflMI CCANNNNNTGG 1 cut(s) 1195
PkrI GCNGC 8 cut(s) 97, 584, 593, 616, 628, 796, 1084, 1237
PleI GAGTC 2 cut(s) 99, 239
PmaCI CACGTG 1 cut(s) 1376
PmlI CACGTG 1 cut(s) 1376
PpsI GAGTC 2 cut(s) 99, 239
Ppu21I YACGTR 1 cut(s) 1376
Psp6I CCWGG 1 cut(s) 1399
PspCI CACGTG 1 cut(s) 1376
PspGI CCWGG 1 cut(s) 1399
PspN4I GGNNCC 6 cut(s) 300, 391, 643, 937, 1396, 1440
PspPI GGNCC 3 cut(s) 291, 694, 767
PspXI VCTCGAGB 1 cut(s) 169
PsrI GAACNNNNNNTAC 2 cut(s) 1082, 1114
PstNI CAGNNNCTG 2 cut(s) 794, 1195
PsuI RGATCY 1 cut(s) 407
PvuII CAGCTG 3 cut(s) 1238, 1418, 1445
RsaI GTAC 3 cut(s) 643, 863, 1380
RsaNI GTAC 3 cut(s) 642, 862, 1379
RseI CAYNNNNRTG 1 cut(s) 23
SaqAI TTAA 1 cut(s) 1112
SatI GCNGC 8 cut(s) 96, 583, 592, 615, 627, 795, 1083, 1236
Sau3AI GATC 4 cut(s) 133, 407, 822, 1151
Sau96I GGNCC 3 cut(s) 291, 694, 767
SchI GAGTC 2 cut(s) 99, 240
ScrFI CCNGG 3 cut(s) 218, 1145, 1401
SduI GDGCHC 1 cut(s) 1399
SfaNI GCATC 5 cut(s) 241, 415, 781, 799, 1333
Sfr274I CTCGAG 1 cut(s) 169
SinI GGWCC 1 cut(s) 291
SlaI CTCGAG 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 3 cut(s) 169, 484, 586
SmoI CTYRAG 3 cut(s) 169, 484, 586
SpeI ACTAGT 1 cut(s) 719
SsiI CCGC 7 cut(s) 100, 384, 448, 543, 582, 594, 629
SspI AATATT 1 cut(s) 188
SspMI CTAG 6 cut(s) 236, 480, 720, 1065, 1340, 1555
StyD4I CCNGG 3 cut(s) 216, 1143, 1399
StyI CCWWGG 4 cut(s) 24, 294, 363, 1155
TaaI ACNGT 5 cut(s) 501, 709, 763, 784, 888
TaiI ACGT 1 cut(s) 1378
TaqI TCGA 4 cut(s) 170, 724, 825, 1491
TaqII GACCGA 2 cut(s) 701, 1239
TatI WGTACW 2 cut(s) 861, 1378
TauI GCSGC 1 cut(s) 585
TfiI GAWTC 1 cut(s) 1285
Tru1I TTAA 1 cut(s) 1112
Tru9I TTAA 1 cut(s) 1112
TscAI CASTG 3 cut(s) 25, 309, 615
TseFI GTSAC 4 cut(s) 306, 495, 1324, 1371
TseI GCWGC 7 cut(s) 95, 591, 614, 626, 794, 1082, 1235
Tsp45I GTSAC 4 cut(s) 306, 495, 1324, 1371
TspDTI ATGAA 3 cut(s) 389, 1089, 1530
TspGWI ACGGA 1 cut(s) 918
TspRI CASTG 3 cut(s) 25, 309, 615
Van91I CCANNNNNTGG 1 cut(s) 1195
VpaK11BI GGWCC 1 cut(s) 291
XapI RAATTY 1 cut(s) 32
XbaI TCTAGA 1 cut(s) 1064
XhoI CTCGAG 1 cut(s) 169
XmiI GTMKAC 2 cut(s) 509, 1026
XspI CTAG 6 cut(s) 236, 480, 720, 1065, 1340, 1555
Zsp2I ATGCAT 1 cut(s) 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.