Rorug06G0047100

Protein RMD5 homolog A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010769
Physical Location & Seq
Forward (+)
6230594 .. 6231253
660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0047100.1

Sequence Viewer

Length: 558 bp
ATGTTGTGGAATATATGGAAGCATCGATGTTCCTCTGTATTCAATTTTTCTCCTCCAGACCCGGTGTCAACTGCAAACAAGGCCTTCATGCAAGCTGAAGAATTCCTCTTGGCTCATGCAACTTGGAACTCTGAGTCTTTGTGTTGTGGTCTTGCTGCGCTTCTCAGAGATTCTTCAGGTACTGTTCTCAATGGTGTGGTCTCAGTGGGGACGGCTACCTCGGCGGAGGCAACGGAGGCTCAAGCGATTCTTCTTGGCCTCAATTTGGCAAAAACTGAGTCCATTCTATCCTTCTCACTTGAAGCTGATTGTCTCCCCTTACTCTCAGCTTTGAAATCCTCTAAGGGATCGGTTTCTTGGTCTACACTCCCTTGGTTTCAACAGATCAAGTCTTTGGCTGATTCTTTCTCTTCTGTAGACTGGTCTTGGATCAGTAGGGAAGCAGATGTTGCTGCACACCTTGCTCCACAGTTGGAGTGTCTGTTGGATTGGTTTATGAACCCGCCTCCTTCCTTGCTTCATGTACTTCAATCAGATGCGGTGGCAGCTCCACCTTGA

Protein Analysis

185

Amino Acids

19.95

Weight (kDa)

4.77

Isoelectric Point (pI)

47.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 40 - 154 1.5e-16 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 362, 417
AciI CCGC 3 cut(s) 224, 503, 539
AclWI GGATC 2 cut(s) 355, 437
AcsI RAATTY 1 cut(s) 101
AcuI CTGAAG 2 cut(s) 117, 159
AfaI GTAC 2 cut(s) 181, 525
AfiI CCNNNNNNNGG 1 cut(s) 265
AgsI TTSAA 5 cut(s) 43, 302, 334, 380, 530
AhdI GACNNNNNGTC 1 cut(s) 64
AluBI AGCT 4 cut(s) 95, 305, 329, 548
AluI AGCT 4 cut(s) 95, 305, 329, 548
Alw26I GTCTC 2 cut(s) 205, 317
AlwI GGATC 2 cut(s) 355, 437
AlwNI CAGNNNCTG 1 cut(s) 182
AoxI GGCC 2 cut(s) 81, 256
ApeKI GCWGC 3 cut(s) 155, 452, 545
ApoI RAATTY 1 cut(s) 101
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
AspLEI GCGC 1 cut(s) 160
AsuC2I CCSGG 1 cut(s) 62
BbvI GCAGC 2 cut(s) 142, 439
BceAI ACGGC 1 cut(s) 228
BcnI CCSGG 1 cut(s) 62
BcoDI GTCTC 2 cut(s) 205, 317
BfmI CTRYAG 1 cut(s) 414
BisI GCNGC 3 cut(s) 156, 453, 546
BlsI GCNGC 3 cut(s) 157, 454, 547
Bme1390I CCNGG 1 cut(s) 62
BmeRI GACNNNNNGTC 1 cut(s) 64
BmrFI CCNGG 1 cut(s) 62
BmsI GCATC 2 cut(s) 31, 526
BpmI CTGGAG 1 cut(s) 39
BpuEI CTTGAG 1 cut(s) 225
BpuMI CCSGG 1 cut(s) 62
Bsa29I ATCGAT 1 cut(s) 25
BsaI GGTCTC 1 cut(s) 205
BsaJI CCNNGG 2 cut(s) 219, 371
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse1I ACTGG 1 cut(s) 425
BseCI ATCGAT 1 cut(s) 25
BseDI CCNNGG 2 cut(s) 219, 371
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMII CTCAG 5 cut(s) 123, 178, 216, 267, 339
BseNI ACTGG 1 cut(s) 425
BseRI GAGGAG 1 cut(s) 42
BseXI GCAGC 2 cut(s) 142, 439
BsgI GTGCAG 1 cut(s) 438
BshFI GGCC 2 cut(s) 83, 258
BshVI ATCGAT 1 cut(s) 25
BsiSI CCGG 1 cut(s) 62
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 2 cut(s) 205, 317
BsmFI GGGAC 1 cut(s) 223
BsnI GGCC 2 cut(s) 83, 258
Bso31I GGTCTC 1 cut(s) 205
Bsp143I GATC 3 cut(s) 347, 384, 429
BspACI CCGC 3 cut(s) 224, 503, 539
BspANI GGCC 2 cut(s) 83, 258
BspCNI CTCAG 5 cut(s) 124, 177, 215, 268, 338
BspDI ATCGAT 1 cut(s) 25
BspPI GGATC 2 cut(s) 355, 437
BspTNI GGTCTC 1 cut(s) 205
BsrI ACTGG 1 cut(s) 425
BssECI CCNNGG 2 cut(s) 219, 371
BssMI GATC 3 cut(s) 347, 384, 429
BssT1I CCWWGG 1 cut(s) 371
Bst4CI ACNGT 2 cut(s) 184, 471
Bst6I CTCTTC 1 cut(s) 415
BstAPI GCANNNNNTGC 2 cut(s) 449, 461
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 6 cut(s) 132, 164, 202, 276, 325, 342
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 3 cut(s) 350, 387, 432
BstMAI GTCTC 2 cut(s) 205, 317
BstMBI GATC 3 cut(s) 347, 384, 429
BstMWI GCNNNNNNNGC 6 cut(s) 80, 221, 236, 449, 461, 545
BstSCI CCNGG 1 cut(s) 60
BstSFI CTRYAG 1 cut(s) 414
BstV1I GCAGC 2 cut(s) 142, 439
Bsu15I ATCGAT 1 cut(s) 25
BsuRI GGCC 2 cut(s) 83, 258
BsuTUI ATCGAT 1 cut(s) 25
BtsIMutI CAGTG 1 cut(s) 210
Cac8I GCNNGC 1 cut(s) 93
CaiI CAGNNNCTG 1 cut(s) 182
CfoI GCGC 1 cut(s) 160
ClaI ATCGAT 1 cut(s) 25
Csp6I GTAC 2 cut(s) 180, 524
CviAII CATG 3 cut(s) 88, 116, 521
CviQI GTAC 2 cut(s) 180, 524
DdeI CTNAG 6 cut(s) 132, 164, 202, 276, 325, 342
DpnI GATC 3 cut(s) 349, 386, 431
DpnII GATC 3 cut(s) 347, 384, 429
DriI GACNNNNNGTC 1 cut(s) 64
Eam1104I CTCTTC 1 cut(s) 415
Eam1105I GACNNNNNGTC 1 cut(s) 64
EarI CTCTTC 1 cut(s) 415
EciI GGCGGA 1 cut(s) 239
Eco130I CCWWGG 1 cut(s) 371
Eco147I AGGCCT 1 cut(s) 83
Eco31I GGTCTC 1 cut(s) 205
Eco57I CTGAAG 2 cut(s) 117, 159
EcoRI GAATTC 1 cut(s) 101
EcoT14I CCWWGG 1 cut(s) 371
ErhI CCWWGG 1 cut(s) 371
FaeI CATG 3 cut(s) 91, 119, 524
FaiI YATR 6 cut(s) 14, 16, 89, 117, 497, 522
FalI AAGNNNNNCTT 2 cut(s) 234, 266
FaqI GGGAC 1 cut(s) 223
FatI CATG 3 cut(s) 87, 115, 520
FauI CCCGC 1 cut(s) 510
FblI GTMKAC 2 cut(s) 362, 417
Fnu4HI GCNGC 3 cut(s) 156, 453, 546
Fsp4HI GCNGC 3 cut(s) 156, 453, 546
GlaI GCGC 1 cut(s) 159
GluI GCNGC 3 cut(s) 156, 453, 546
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 2 cut(s) 83, 258
HapII CCGG 1 cut(s) 62
HhaI GCGC 1 cut(s) 160
Hin1II CATG 3 cut(s) 91, 119, 524
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HincII GTYRAC 1 cut(s) 69
HindII GTYRAC 1 cut(s) 69
HinfI GANTC 5 cut(s) 134, 170, 247, 278, 401
HpaII CCGG 1 cut(s) 62
Hpy166II GTNNAC 3 cut(s) 69, 363, 418
Hpy188I TCNGA 3 cut(s) 133, 167, 535
Hpy188III TCNNGA 1 cut(s) 56
Hpy8I GTNNAC 3 cut(s) 69, 363, 418
HpyAV CCTTC 3 cut(s) 94, 301, 519
HpyCH4III ACNGT 2 cut(s) 184, 471
HpyCH4V TGCA 4 cut(s) 74, 91, 119, 455
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 221, 236, 449, 461, 545
HpyF3I CTNAG 6 cut(s) 132, 164, 202, 276, 325, 342
Hsp92II CATG 3 cut(s) 91, 119, 524
HspAI GCGC 1 cut(s) 158
Kzo9I GATC 3 cut(s) 347, 384, 429
LmnI GCTCC 2 cut(s) 469, 553
LpnPI CCDG 4 cut(s) 69, 75, 162, 406
Lsp1109I GCAGC 2 cut(s) 142, 439
LweI GCATC 2 cut(s) 31, 526
MalI GATC 3 cut(s) 349, 386, 431
MboI GATC 3 cut(s) 347, 384, 429
MboII GAAGA 4 cut(s) 110, 165, 242, 402
MluCI AATT 3 cut(s) 43, 101, 262
MlyI GAGTC 2 cut(s) 143, 287
MmeI TCCRAC 2 cut(s) 453, 465
MnlI CCTC 9 cut(s) 43, 63, 116, 220, 229, 229, 269, 349, 516
MspI CCGG 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 62
MwoI GCNNNNNNNGC 6 cut(s) 80, 221, 236, 449, 461, 545
NciI CCSGG 1 cut(s) 62
NdeII GATC 3 cut(s) 347, 384, 429
NlaIII CATG 3 cut(s) 91, 119, 524
NmeAIII GCCGAG 1 cut(s) 200
PceI AGGCCT 1 cut(s) 83
PfeI GAWTC 3 cut(s) 170, 247, 401
PkrI GCNGC 3 cut(s) 157, 454, 547
PleI GAGTC 2 cut(s) 142, 286
PpsI GAGTC 2 cut(s) 142, 286
PstNI CAGNNNCTG 1 cut(s) 182
RsaI GTAC 2 cut(s) 181, 525
RsaNI GTAC 2 cut(s) 180, 524
SatI GCNGC 3 cut(s) 156, 453, 546
Sau3AI GATC 3 cut(s) 347, 384, 429
SchI GAGTC 2 cut(s) 143, 287
ScrFI CCNGG 1 cut(s) 62
SetI ASST 8 cut(s) 97, 181, 221, 307, 331, 462, 550, 556
SfaNI GCATC 2 cut(s) 31, 526
SfcI CTRYAG 1 cut(s) 414
SmlI CTYRAG 1 cut(s) 240
SmoI CTYRAG 1 cut(s) 240
Sse9I AATT 3 cut(s) 43, 101, 262
SseBI AGGCCT 1 cut(s) 83
SsiI CCGC 3 cut(s) 224, 503, 539
StuI AGGCCT 1 cut(s) 83
StyD4I CCNGG 1 cut(s) 60
StyI CCWWGG 1 cut(s) 371
TaaI ACNGT 2 cut(s) 184, 471
TaqI TCGA 1 cut(s) 25
TasI AATT 3 cut(s) 43, 101, 262
TatI WGTACW 1 cut(s) 523
TfiI GAWTC 3 cut(s) 170, 247, 401
TscAI CASTG 1 cut(s) 210
TseI GCWGC 3 cut(s) 155, 452, 545
TspDTI ATGAA 3 cut(s) 76, 509, 512
TspGWI ACGGA 1 cut(s) 248
TspRI CASTG 1 cut(s) 210
XapI RAATTY 1 cut(s) 101
XmiI GTMKAC 2 cut(s) 362, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.