Rorug06G0152400

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0015267
Physical Location & Seq
Forward (+)
21842051 .. 21842671
621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0152400.1

Sequence Viewer

Length: 621 bp
ATGAATCCTGTGGTTTCTGCACTGATTTTATCGGTTCCCACCGGCGTAGTCCTCTGGGTCATAGTTGGCGTACTCGAAGAGAAAATCAGAGTCGCCAGACCTGGATTCGCCCCCGCCATAGATATCGCTGTGAGTATACTCACGATACTAGTTATTTTTCTTTTCCGCACCATTGCGCTTGGTACCATTTCCATTGTTGAACCCTCCCTCGATTACAGGATCGTAACAGGCCTCGGCTATGGGTTTTATATCCCAATGTTACCCTGGGTATATGACATAATAACCACCGTTGGCTCATGTTGTTCAAGAACAATGGTTAAACTGATGCACAATGCTACTTTTCTTATGGTGACAATCGGTGCCAAGAGCCCTGAAGGCTATGCGATTTTCAATTATCAAGTGGACCAAGAAAAGGGGTTTTGCAGAGGCGAGTGTGCCATTTGCTTGATGGAGTACGAGACAAAAGACAAGTGTGCATTGTTAGATAAATGTGGACATACGTTTCACCACAAATGTCTATATGAGAATTTGGCTATGTCTACCCGATGTCCGCTTTGTCGTCAAGTATCAAAGATAGTAAGGAACTCCATGCAGCTGCCAGTCAACCTAGATGCTAATTAA

Protein Analysis

206

Amino Acids

22.86

Weight (kDa)

8.02

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 141 - 187 9.6e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 144 - 187 1.3e-12 Ring finger domain
zf-RING_11 PF17123 144 - 173 6.7e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 144 - 186 2e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 144 - 188 2.6e-06 zinc-RING finger domain
zf-RING_UBOX PF13445 145 - 184 6.2e-07 RING-type zinc-finger
zf-C3HC4 PF00097 145 - 186 9.3e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015267)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12970
fragaria_vesca FvH4_2g17950
malus_domestica MD05G1163400.v1.1 MD10G1153200.v1.1
prunus_persica Prupe.8G192000_v2.0.a1
pyrus_communis pycom05g15280
rosa_chinensis RchiOBHm_Chr6g0283181
rosa_laevigata RLG00000012838
rosa_multiflora Rmu_sc0014685.1_g000009
rosa_roxburghii Rroxscaffold_7G00185680
rosa_rugosa Rorug06G0152400
rosa_samantha Rh6AG264900 Rh6BG267800 Rh6CG266800 Rh6DG260100
rosa_wichuraiana Rw6G022940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 182
AccB1I GGYRCC 2 cut(s) 182, 359
AccI GTMKAC 2 cut(s) 136, 539
AciI CCGC 3 cut(s) 114, 166, 551
AclWI GGATC 1 cut(s) 227
AcsI RAATTY 1 cut(s) 526
AcuI CTGAAG 1 cut(s) 393
AfaI GTAC 3 cut(s) 72, 184, 455
AfiI CCNNNNNNNGG 1 cut(s) 412
AgsI TTSAA 3 cut(s) 200, 306, 391
AhlI ACTAGT 1 cut(s) 148
AjnI CCWGG 2 cut(s) 100, 263
AluBI AGCT 1 cut(s) 595
AluI AGCT 1 cut(s) 595
Alw26I GTCTC 1 cut(s) 452
AlwI GGATC 1 cut(s) 227
AoxI GGCC 1 cut(s) 229
ApeKI GCWGC 2 cut(s) 592, 595
ApoI RAATTY 1 cut(s) 526
Asp718I GGTACC 1 cut(s) 182
AspLEI GCGC 1 cut(s) 178
AspS9I GGNCC 1 cut(s) 403
AsuHPI GGTGA 2 cut(s) 361, 497
AvaII GGWCC 1 cut(s) 403
BanI GGYRCC 2 cut(s) 182, 359
BanII GRGCYC 1 cut(s) 371
BbvI GCAGC 2 cut(s) 582, 604
BccI CCATC 1 cut(s) 442
BciT130I CCWGG 2 cut(s) 102, 265
BcoDI GTCTC 1 cut(s) 452
BcuI ACTAGT 1 cut(s) 148
BfaI CTAG 2 cut(s) 149, 608
BglI GCCNNNNNGGC 1 cut(s) 375
BisI GCNGC 2 cut(s) 593, 596
BlsI GCNGC 2 cut(s) 594, 597
Bme1390I CCNGG 2 cut(s) 102, 265
Bme18I GGWCC 1 cut(s) 403
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 3 cut(s) 36, 184, 361
BmrFI CCNGG 2 cut(s) 102, 265
BmsI GCATC 2 cut(s) 315, 601
BsaJI CCNNGG 3 cut(s) 232, 263, 264
Bsc4I CCNNNNNNNGG 1 cut(s) 412
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 1 cut(s) 599
Bse3DI GCAATG 1 cut(s) 171
BseBI CCWGG 2 cut(s) 102, 265
BseDI CCNNGG 3 cut(s) 232, 263, 264
BseLI CCNNNNNNNGG 1 cut(s) 412
BseMI GCAATG 1 cut(s) 171
BseNI ACTGG 1 cut(s) 599
BseXI GCAGC 2 cut(s) 582, 604
BshFI GGCC 1 cut(s) 231
BshNI GGYRCC 2 cut(s) 182, 359
BsiSI CCGG 1 cut(s) 42
BslI CCNNNNNNNGG 1 cut(s) 412
BsmAI GTCTC 1 cut(s) 452
BsnI GGCC 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 371
Bsp143I GATC 1 cut(s) 219
BspACI CCGC 3 cut(s) 114, 166, 551
BspANI GGCC 1 cut(s) 231
BspLI GGNNCC 3 cut(s) 36, 184, 361
BspPI GGATC 1 cut(s) 227
BspT107I GGYRCC 2 cut(s) 182, 359
BsrDI GCAATG 1 cut(s) 171
BsrFI RCCGGY 1 cut(s) 41
BsrI ACTGG 1 cut(s) 599
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 3 cut(s) 232, 263, 264
BssMI GATC 1 cut(s) 219
BssNAI GTATAC 1 cut(s) 137
Bst1107I GTATAC 1 cut(s) 137
Bst2UI CCWGG 2 cut(s) 102, 265
Bst4CI ACNGT 1 cut(s) 289
Bst6I CTCTTC 1 cut(s) 72
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 1 cut(s) 222
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 1 cut(s) 219
BstMWI GCNNNNNNNGC 1 cut(s) 375
BstNI CCWGG 2 cut(s) 102, 265
BstSCI CCNGG 2 cut(s) 100, 263
BstV1I GCAGC 2 cut(s) 582, 604
BstZ17I GTATAC 1 cut(s) 137
BsuRI GGCC 1 cut(s) 231
BtsIMutI CAGTG 1 cut(s) 20
CfoI GCGC 1 cut(s) 178
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 1 cut(s) 403
Csp6I GTAC 3 cut(s) 71, 183, 454
CviAII CATG 2 cut(s) 297, 589
CviJI RGCY 7 cut(s) 231, 237, 294, 369, 378, 533, 595
CviKI_1 RGCY 7 cut(s) 231, 237, 294, 369, 378, 533, 595
CviQI GTAC 3 cut(s) 71, 183, 454
DpnI GATC 1 cut(s) 221
DpnII GATC 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
Eco147I AGGCCT 1 cut(s) 231
Eco24I GRGCYC 1 cut(s) 371
Eco32I GATATC 1 cut(s) 124
Eco47I GGWCC 1 cut(s) 403
Eco57I CTGAAG 1 cut(s) 393
EcoRII CCWGG 2 cut(s) 100, 263
EcoRV GATATC 1 cut(s) 124
EcoT38I GRGCYC 1 cut(s) 371
FaeI CATG 2 cut(s) 300, 592
FatI CATG 2 cut(s) 296, 588
FauI CCCGC 1 cut(s) 121
FblI GTMKAC 2 cut(s) 136, 539
Fnu4HI GCNGC 2 cut(s) 593, 596
FriOI GRGCYC 1 cut(s) 371
Fsp4HI GCNGC 2 cut(s) 593, 596
FspBI CTAG 2 cut(s) 149, 608
GlaI GCGC 1 cut(s) 177
GluI GCNGC 2 cut(s) 593, 596
HaeIII GGCC 1 cut(s) 231
HapII CCGG 1 cut(s) 42
HhaI GCGC 1 cut(s) 178
Hin1II CATG 2 cut(s) 300, 592
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HinfI GANTC 3 cut(s) 4, 90, 105
HpaII CCGG 1 cut(s) 42
HphI GGTGA 2 cut(s) 361, 497
Hpy166II GTNNAC 5 cut(s) 137, 403, 494, 540, 604
Hpy188I TCNGA 1 cut(s) 89
Hpy188III TCNNGA 2 cut(s) 142, 306
Hpy8I GTNNAC 5 cut(s) 137, 403, 494, 540, 604
HpyAV CCTTC 1 cut(s) 368
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 500
HpyCH4V TGCA 5 cut(s) 20, 328, 423, 476, 592
HpyF10VI GCNNNNNNNGC 1 cut(s) 375
HpySE526I ACGT 1 cut(s) 500
Hsp92II CATG 2 cut(s) 300, 592
HspAI GCGC 1 cut(s) 176
KpnI GGTACC 1 cut(s) 186
Kzo9I GATC 1 cut(s) 219
Lsp1109I GCAGC 2 cut(s) 582, 604
LweI GCATC 2 cut(s) 315, 601
MaeI CTAG 2 cut(s) 149, 608
MaeII ACGT 1 cut(s) 500
MaeIII GTNAC 3 cut(s) 223, 258, 349
MalI GATC 1 cut(s) 221
MboI GATC 1 cut(s) 219
MboII GAAGA 1 cut(s) 89
MhlI GDGCHC 1 cut(s) 371
MluCI AATT 3 cut(s) 391, 526, 616
MlyI GAGTC 1 cut(s) 99
MnlI CCTC 5 cut(s) 62, 214, 218, 242, 419
MseI TTAA 2 cut(s) 318, 619
MspA1I CMGCKG 1 cut(s) 595
MspI CCGG 1 cut(s) 42
MspR9I CCNGG 2 cut(s) 102, 265
MvaI CCWGG 2 cut(s) 102, 265
MwoI GCNNNNNNNGC 1 cut(s) 375
NdeII GATC 1 cut(s) 219
NlaIII CATG 2 cut(s) 300, 592
NlaIV GGNNCC 3 cut(s) 36, 184, 361
NmeAIII GCCGAG 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 349
PasI CCCWGGG 1 cut(s) 264
PceI AGGCCT 1 cut(s) 231
PfeI GAWTC 2 cut(s) 4, 105
PkrI GCNGC 2 cut(s) 594, 597
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
Psp6I CCWGG 2 cut(s) 100, 263
PspGI CCWGG 2 cut(s) 100, 263
PspN4I GGNNCC 3 cut(s) 36, 184, 361
PspPI GGNCC 1 cut(s) 403
PvuII CAGCTG 1 cut(s) 595
RsaI GTAC 3 cut(s) 72, 184, 455
RsaNI GTAC 3 cut(s) 71, 183, 454
SaqAI TTAA 2 cut(s) 318, 619
SatI GCNGC 2 cut(s) 593, 596
Sau3AI GATC 1 cut(s) 219
Sau96I GGNCC 1 cut(s) 403
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 2 cut(s) 102, 265
SduI GDGCHC 1 cut(s) 371
SetI ASST 4 cut(s) 103, 503, 597, 609
SfaNI GCATC 2 cut(s) 315, 601
SgrAI CRCCGGYG 1 cut(s) 41
SinI GGWCC 1 cut(s) 403
SpeI ACTAGT 1 cut(s) 148
Sse9I AATT 3 cut(s) 391, 526, 616
SseBI AGGCCT 1 cut(s) 231
SsiI CCGC 3 cut(s) 114, 166, 551
SspMI CTAG 2 cut(s) 149, 608
StuI AGGCCT 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 100, 263
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 1 cut(s) 503
TaqI TCGA 2 cut(s) 75, 210
TasI AATT 3 cut(s) 391, 526, 616
TfiI GAWTC 2 cut(s) 4, 105
Tru1I TTAA 2 cut(s) 318, 619
Tru9I TTAA 2 cut(s) 318, 619
TscAI CASTG 1 cut(s) 27
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 2 cut(s) 592, 595
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 27
VpaK11BI GGWCC 1 cut(s) 403
XapI RAATTY 1 cut(s) 526
XcmI CCANNNNNNNNNTGG 2 cut(s) 261, 445
XmiI GTMKAC 2 cut(s) 136, 539
XspI CTAG 2 cut(s) 149, 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.