Rorug06G0303300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0014485
Physical Location & Seq
Forward (+)
45454331 .. 45457350
3020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0303300.1

Sequence Viewer

Length: 1203 bp
ATGGACAAGTTTAATTTAGCTCAGTTGGGTAACTTGGGCGAGAAATTGGTGACGAGTGGAGCTCAAATGGGTCGGATGGTTAGTGGCAAAGTGAAGGAGATTCTGGCTACCCCGACTCCGGAATCGAGGATGATTGATGAGGCCACCTTGGAGACAATGGAGGAGCCCAATTGGGGCTTGAATTTGAGGATATGTGCTATGATTAACAGTGAGGAGTTTAGTGGGTCGGAAGTTGTGAGGGTTATAAAGAGGAAGATTTCGGGGAAGAATGTTGTGACACAGAGGCTGAGTCTTGACTTGTTGGAGGCGTGTACTATGAATTGCGAGAAGGTGTTCTCGGAGGTTGCGTCGGAGAAGGTGTTGGATGAGATGCTTAGGATGATTGATAATCCACAGACTGATAGCGAAAATAGACGCAGGGCTATGCAGTTGATAAGGGCTTGGGGAGAGTCTCAGGATCTTGCATATCTACCTGTATTTCGTCAGACTTACATGATCTTGAGAGAAAAAGGAGCACATCCAGTAGCACAAGGAGGGACTTCGCCCTCGTTGCAATCTAGCTTGGAATCATACATGGAAGAGCCTCTGCCTCCCCCGGAAAGATACCCTGTACCTGACACAGGATTTCATGATGCAAGTGACCGTGAATTTCGCTATAACTACCTGTCAGTTGAGGAAAAAAAAGAATATCTTGTTGTAACTCGCAATAGCCTGGAGCTGCTGTCTAGTATTTTGAATACTGAAACAGATCCAAAACCTTTGAAGGAAGAACTAACCATGAACATGCTGGAAAAGTGCAAGGAGTCTCAGCCTGTTATTCAGAGGATTATAGAAACCACTACTGATGATGAAGGGATGCTCTTTGAGGCACTATTTCTTCATGAAGAACTTCAACAAGTTATTTCCAAGTATCAGGAGTTGGAAGCTTCTCAAAACTCTGGAGGCCAACAGCTCGAAAACCCGGATGCCACCAGGCCTGAGGGGTCAGAAGCGGCTCAAAATGGAGAACTACCAGTAAGCTCTAACCCCCCTGAGCGTGAGGATTTAGATAACAGCCAAAAACATGGTGGTAAACTGCCTGAATATTCCAATGATTCTGAAGTTGGCCCATCAGCTCCTCTGGGAATTCATAGTGAAACCAAAATAGCAGATTCTGTACAAGAAGGCACTCCTCCGTCTATCAGTGAAAAAGTTGGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

400

Amino Acids

44.7

Weight (kDa)

4.7

Isoelectric Point (pI)

50.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VHS PF00790 42 - 151 3.8e-21 VHS domain
GAT PF03127 235 - 308 3.8e-16 GAT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014485)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42785
malus_domestica MD08G1029300.v1.1 MD15G1024800.v1.1
prunus_persica Prupe.1G377200_v2.0.a1
pyrus_communis pycom08g02310 pycom15g02130
rosa_chinensis RchiOBHm_Chr6g0301311
rosa_laevigata RLG00000011260
rosa_multiflora Rmu_sc0003197.1_g000009 Rmu_sc0013354.1_g000019
rosa_roxburghii Rroxscaffold_7G00166930
rosa_rugosa Rorug06G0303300
rosa_samantha Rh6AG417100 Rh6BG423700 Rh6CG430900 Rh6DG416800
rosa_wichuraiana Rw6G036090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 245
AccIII TCCGGA 1 cut(s) 118
AciI CCGC 1 cut(s) 992
AclWI GGATC 2 cut(s) 465, 743
AcoI YGGCCR 1 cut(s) 1195
AcsI RAATTY 3 cut(s) 181, 647, 1125
AcuI CTGAAG 1 cut(s) 1119
AfaI GTAC 3 cut(s) 313, 612, 1158
AfiI CCNNNNNNNGG 3 cut(s) 118, 173, 620
AgsI TTSAA 4 cut(s) 181, 736, 763, 893
AjnI CCWGG 2 cut(s) 711, 971
AluBI AGCT 8 cut(s) 20, 62, 561, 718, 926, 952, 1020, 1115
AluI AGCT 8 cut(s) 20, 62, 561, 718, 926, 952, 1020, 1115
Alw21I GWGCWC 2 cut(s) 64, 517
Alw26I GTCTC 3 cut(s) 146, 456, 810
AlwI GGATC 2 cut(s) 465, 743
AlwNI CAGNNNCTG 2 cut(s) 286, 1154
Aor13HI TCCGGA 1 cut(s) 118
AoxI GGCC 5 cut(s) 141, 943, 974, 1105, 1195
ApeKI GCWGC 1 cut(s) 718
ApoI RAATTY 3 cut(s) 181, 647, 1125
Asp700I GAANNNNTTC 2 cut(s) 332, 888
AspS9I GGNCC 1 cut(s) 1106
AsuC2I CCSGG 2 cut(s) 596, 962
AsuHPI GGTGA 1 cut(s) 61
AxyI CCTNAGG 1 cut(s) 978
BalI TGGCCA 1 cut(s) 1197
BanII GRGCYC 2 cut(s) 64, 168
Bbv12I GWGCWC 2 cut(s) 64, 517
BbvI GCAGC 1 cut(s) 705
BccI CCATC 2 cut(s) 70, 1117
BciT130I CCWGG 2 cut(s) 713, 973
BcnI CCSGG 2 cut(s) 596, 962
BcoDI GTCTC 3 cut(s) 146, 456, 810
BfaI CTAG 2 cut(s) 558, 726
BisI GCNGC 2 cut(s) 719, 993
BlsI GCNGC 2 cut(s) 720, 994
Bme1390I CCNGG 4 cut(s) 596, 713, 962, 973
BmgT120I GGNCC 1 cut(s) 1106
BmiI GGNNCC 1 cut(s) 165
BmrFI CCNGG 4 cut(s) 596, 713, 962, 973
BmsI GCATC 4 cut(s) 360, 622, 846, 955
BplI GAGNNNNNCTC 2 cut(s) 46, 78
BpmI CTGGAG 2 cut(s) 734, 960
Bpu10I CCTNAGC 2 cut(s) 374, 1032
BpuEI CTTGAG 1 cut(s) 520
BpuMI CCSGG 2 cut(s) 596, 962
BsaJI CCNNGG 2 cut(s) 147, 594
BsaWI WCCGGW 1 cut(s) 118
BsaXI ACNNNNNCTCC 8 cut(s) 100, 130, 332, 344, 362, 374, 707, 737
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 173, 620
Bse1I ACTGG 2 cut(s) 521, 1013
Bse21I CCTNAGG 1 cut(s) 978
BseAI TCCGGA 1 cut(s) 118
BseBI CCWGG 2 cut(s) 713, 973
BseDI CCNNGG 2 cut(s) 147, 594
BseGI GGATG 7 cut(s) 81, 135, 370, 384, 517, 861, 970
BseLI CCNNNNNNNGG 3 cut(s) 118, 173, 620
BseMII CTCAG 6 cut(s) 35, 278, 467, 821, 969, 1023
BseNI ACTGG 2 cut(s) 521, 1013
BseRI GAGGAG 4 cut(s) 176, 227, 1107, 1161
BseXI GCAGC 1 cut(s) 705
BshFI GGCC 5 cut(s) 143, 945, 976, 1107, 1197
BsiHKAI GWGCWC 2 cut(s) 64, 517
BsiSI CCGG 3 cut(s) 119, 596, 962
BslFI GGGAC 1 cut(s) 550
BslI CCNNNNNNNGG 3 cut(s) 118, 173, 620
BsmAI GTCTC 3 cut(s) 146, 456, 810
BsmFI GGGAC 1 cut(s) 550
BsnI GGCC 5 cut(s) 143, 945, 976, 1107, 1197
Bsp1286I GDGCHC 3 cut(s) 64, 168, 517
Bsp13I TCCGGA 1 cut(s) 118
Bsp1407I TGTACA 1 cut(s) 1156
Bsp143I GATC 3 cut(s) 457, 495, 748
BspACI CCGC 1 cut(s) 992
BspANI GGCC 5 cut(s) 143, 945, 976, 1107, 1197
BspCNI CTCAG 6 cut(s) 34, 279, 466, 820, 970, 1024
BspEI TCCGGA 1 cut(s) 118
BspHI TCATGA 2 cut(s) 628, 880
BspLI GGNNCC 1 cut(s) 165
BspPI GGATC 2 cut(s) 465, 743
BspQI GCTCTTC 1 cut(s) 573
BsrGI TGTACA 1 cut(s) 1156
BsrI ACTGG 2 cut(s) 521, 1013
BssECI CCNNGG 2 cut(s) 147, 594
BssMI GATC 3 cut(s) 457, 495, 748
BssT1I CCWWGG 1 cut(s) 147
Bst2UI CCWGG 2 cut(s) 713, 973
Bst4CI ACNGT 2 cut(s) 209, 644
Bst6I CTCTTC 1 cut(s) 573
BstAUI TGTACA 1 cut(s) 1156
BstDEI CTNAG 7 cut(s) 21, 287, 374, 453, 807, 978, 1032
BstENI CCTNNNNNAGG 1 cut(s) 618
BstF5I GGATG 7 cut(s) 81, 135, 370, 384, 517, 861, 970
BstKTI GATC 3 cut(s) 460, 498, 751
BstMAI GTCTC 3 cut(s) 146, 456, 810
BstMBI GATC 3 cut(s) 457, 495, 748
BstMWI GCNNNNNNNGC 1 cut(s) 550
BstNI CCWGG 2 cut(s) 713, 973
BstNSI RCATGY 1 cut(s) 787
BstSCI CCNGG 4 cut(s) 594, 711, 960, 971
BstV1I GCAGC 1 cut(s) 705
BstX2I RGATCY 2 cut(s) 457, 748
BstXI CCANNNNNNTGG 1 cut(s) 1064
BstYI RGATCY 2 cut(s) 457, 748
Bsu36I CCTNAGG 1 cut(s) 978
BsuRI GGCC 5 cut(s) 143, 945, 976, 1107, 1197
BtsCI GGATG 7 cut(s) 81, 135, 370, 384, 517, 861, 970
BtsIMutI CAGTG 2 cut(s) 214, 1189
CaiI CAGNNNCTG 2 cut(s) 286, 1154
CciI TCATGA 2 cut(s) 628, 880
Cfr13I GGNCC 1 cut(s) 1106
CseI GACGC 2 cut(s) 336, 423
Csp6I GTAC 3 cut(s) 312, 611, 1157
CviAII CATG 7 cut(s) 493, 574, 629, 778, 784, 881, 1064
CviQI GTAC 3 cut(s) 312, 611, 1157
DdeI CTNAG 7 cut(s) 21, 287, 374, 453, 807, 978, 1032
DpnI GATC 3 cut(s) 459, 497, 750
DpnII GATC 3 cut(s) 457, 495, 748
EaeI YGGCCR 1 cut(s) 1195
Eam1104I CTCTTC 1 cut(s) 573
EarI CTCTTC 1 cut(s) 573
Ecl136II GAGCTC 1 cut(s) 62
Eco130I CCWWGG 1 cut(s) 147
Eco147I AGGCCT 1 cut(s) 976
Eco24I GRGCYC 2 cut(s) 64, 168
Eco53kI GAGCTC 1 cut(s) 62
Eco57I CTGAAG 1 cut(s) 1119
Eco81I CCTNAGG 1 cut(s) 978
EcoICRI GAGCTC 1 cut(s) 62
EcoNI CCTNNNNNAGG 1 cut(s) 618
EcoRI GAATTC 1 cut(s) 1125
EcoRII CCWGG 2 cut(s) 711, 971
EcoT14I CCWWGG 1 cut(s) 147
EcoT38I GRGCYC 2 cut(s) 64, 168
ErhI CCWWGG 1 cut(s) 147
FaeI CATG 7 cut(s) 496, 577, 632, 781, 787, 884, 1067
FalI AAGNNNNNCTT 2 cut(s) 675, 707
FaqI GGGAC 1 cut(s) 550
FatI CATG 7 cut(s) 492, 573, 628, 777, 783, 880, 1063
Fnu4HI GCNGC 2 cut(s) 719, 993
FokI GGATG 7 cut(s) 88, 142, 377, 391, 504, 868, 977
FriOI GRGCYC 2 cut(s) 64, 168
Fsp4HI GCNGC 2 cut(s) 719, 993
FspBI CTAG 2 cut(s) 558, 726
GluI GCNGC 2 cut(s) 719, 993
GsuI CTGGAG 2 cut(s) 734, 960
HaeIII GGCC 5 cut(s) 143, 945, 976, 1107, 1197
HapII CCGG 3 cut(s) 119, 596, 962
HgaI GACGC 2 cut(s) 336, 423
Hin1II CATG 7 cut(s) 496, 577, 632, 781, 787, 884, 1067
HindIII AAGCTT 1 cut(s) 924
HinfI GANTC 9 cut(s) 100, 115, 122, 289, 449, 566, 803, 1094, 1151
HpaII CCGG 3 cut(s) 119, 596, 962
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 2 cut(s) 312, 1073
Hpy188I TCNGA 8 cut(s) 75, 229, 340, 352, 486, 822, 988, 1099
Hpy188III TCNNGA 8 cut(s) 119, 293, 455, 499, 629, 881, 914, 939
Hpy8I GTNNAC 2 cut(s) 312, 1073
Hpy99I CGWCG 1 cut(s) 352
HpyAV CCTTC 6 cut(s) 88, 322, 349, 757, 845, 1157
HpyCH4III ACNGT 2 cut(s) 209, 644
HpyCH4V TGCA 5 cut(s) 427, 464, 553, 635, 798
HpyF10VI GCNNNNNNNGC 1 cut(s) 550
HpyF3I CTNAG 7 cut(s) 21, 287, 374, 453, 807, 978, 1032
Hsp92II CATG 7 cut(s) 496, 577, 632, 781, 787, 884, 1067
Kpn2I TCCGGA 1 cut(s) 118
Kzo9I GATC 3 cut(s) 457, 495, 748
LguI GCTCTTC 1 cut(s) 573
LmnI GCTCC 5 cut(s) 59, 163, 512, 715, 1120
Lsp1109I GCAGC 1 cut(s) 705
LweI GCATC 4 cut(s) 360, 622, 846, 955
MaeI CTAG 2 cut(s) 558, 726
MaeIII GTNAC 5 cut(s) 29, 49, 274, 638, 697
MalI GATC 3 cut(s) 459, 497, 750
MboI GATC 3 cut(s) 457, 495, 748
MboII GAAGA 6 cut(s) 265, 277, 590, 779, 869, 896
MfeI CAATTG 1 cut(s) 169
MflI RGATCY 2 cut(s) 457, 748
MhlI GDGCHC 3 cut(s) 64, 168, 517
MlsI TGGCCA 1 cut(s) 1197
MluCI AATT 7 cut(s) 13, 44, 169, 181, 319, 647, 1125
MluNI TGGCCA 1 cut(s) 1197
MlyI GAGTC 4 cut(s) 109, 298, 458, 812
MmeI TCCRAC 6 cut(s) 53, 207, 282, 330, 342, 900
Mox20I TGGCCA 1 cut(s) 1197
MroI TCCGGA 1 cut(s) 118
MroXI GAANNNNTTC 2 cut(s) 332, 888
MscI TGGCCA 1 cut(s) 1197
MseI TTAA 2 cut(s) 12, 204
MslI CAYNNNNRTG 1 cut(s) 782
Msp20I TGGCCA 1 cut(s) 1197
MspI CCGG 3 cut(s) 119, 596, 962
MspR9I CCNGG 4 cut(s) 596, 713, 962, 973
MunI CAATTG 1 cut(s) 169
MvaI CCWGG 2 cut(s) 713, 973
MwoI GCNNNNNNNGC 1 cut(s) 550
NciI CCSGG 2 cut(s) 596, 962
NdeII GATC 3 cut(s) 457, 495, 748
NlaIII CATG 7 cut(s) 496, 577, 632, 781, 787, 884, 1067
NlaIV GGNNCC 1 cut(s) 165
NmuCI GTSAC 3 cut(s) 49, 274, 638
NspI RCATGY 1 cut(s) 787
PagI TCATGA 2 cut(s) 628, 880
PceI AGGCCT 1 cut(s) 976
PciSI GCTCTTC 1 cut(s) 573
PcsI WCGNNNNNNNCGW 1 cut(s) 344
PdmI GAANNNNTTC 2 cut(s) 332, 888
PfeI GAWTC 5 cut(s) 100, 122, 566, 1094, 1151
PkrI GCNGC 2 cut(s) 720, 994
PleI GAGTC 4 cut(s) 109, 297, 457, 811
PpsI GAGTC 4 cut(s) 109, 297, 457, 811
PsiI TTATAA 1 cut(s) 245
Psp124BI GAGCTC 1 cut(s) 64
Psp6I CCWGG 2 cut(s) 711, 971
PspGI CCWGG 2 cut(s) 711, 971
PspN4I GGNNCC 1 cut(s) 165
PspPI GGNCC 1 cut(s) 1106
PstNI CAGNNNCTG 2 cut(s) 286, 1154
PsuI RGATCY 2 cut(s) 457, 748
RsaI GTAC 3 cut(s) 313, 612, 1158
RsaNI GTAC 3 cut(s) 312, 611, 1157
RseI CAYNNNNRTG 1 cut(s) 782
SacI GAGCTC 1 cut(s) 64
SapI GCTCTTC 1 cut(s) 573
SaqAI TTAA 2 cut(s) 12, 204
SatI GCNGC 2 cut(s) 719, 993
Sau3AI GATC 3 cut(s) 457, 495, 748
Sau96I GGNCC 1 cut(s) 1106
SchI GAGTC 4 cut(s) 109, 298, 458, 812
ScrFI CCNGG 4 cut(s) 596, 713, 962, 973
SduI GDGCHC 3 cut(s) 64, 168, 517
SfaNI GCATC 4 cut(s) 360, 622, 846, 955
SmiMI CAYNNNNRTG 1 cut(s) 782
SmlI CTYRAG 1 cut(s) 499
SmoI CTYRAG 1 cut(s) 499
Sse9I AATT 7 cut(s) 13, 44, 169, 181, 319, 647, 1125
SseBI AGGCCT 1 cut(s) 976
SsiI CCGC 1 cut(s) 992
SspI AATATT 1 cut(s) 1085
SspMI CTAG 2 cut(s) 558, 726
SstI GAGCTC 1 cut(s) 64
StuI AGGCCT 1 cut(s) 976
StyD4I CCNGG 4 cut(s) 594, 711, 960, 971
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 2 cut(s) 209, 644
TaqI TCGA 2 cut(s) 125, 954
TasI AATT 7 cut(s) 13, 44, 169, 181, 319, 647, 1125
TatI WGTACW 2 cut(s) 311, 1156
TauI GCSGC 1 cut(s) 995
TfiI GAWTC 5 cut(s) 100, 122, 566, 1094, 1151
Tru1I TTAA 2 cut(s) 12, 204
Tru9I TTAA 2 cut(s) 12, 204
TscAI CASTG 2 cut(s) 214, 1189
TseFI GTSAC 3 cut(s) 49, 274, 638
TseI GCWGC 1 cut(s) 718
Tsp45I GTSAC 3 cut(s) 49, 274, 638
TspDTI ATGAA 7 cut(s) 332, 617, 794, 864, 869, 897, 1118
TspGWI ACGGA 1 cut(s) 1164
TspRI CASTG 2 cut(s) 214, 1189
XagI CCTNNNNNAGG 1 cut(s) 618
XapI RAATTY 3 cut(s) 181, 647, 1125
XceI RCATGY 1 cut(s) 787
XcmI CCANNNNNNNNNTGG 2 cut(s) 784, 1064
XmnI GAANNNNTTC 2 cut(s) 332, 888
XspI CTAG 2 cut(s) 558, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.