Rorug06G0305100

Belongs to the DEFL family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0017253
Physical Location & Seq
Reverse (-)
45656610 .. 45660263
3654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0305100.1

Sequence Viewer

Length: 1296 bp
ATGAACTTACTGGAGAAGATAAAGATGCAGAAGAAGGAGCCAGATAAAGCGCCGCCGCCGCCTGAGCTGCCGGCGTACTGGATCGAAACCTCTGAAGCAGTCTCATACCGCTGTGAATTTGAACCCCACGGTCACCTCTCTGTGAAAATTCTTGATGACTCGAGGCCAGTAATCCGTAGGGTTGCAGATTCCTTCGTCAATAAGTTTTTCCCCTCAGGATATCCATATAGTGTCAATGAGGGATATCTCAGGTACACACAATTTCGGGCACTGCAACACTTCACCAGTGCAGCGCTTTCGGTGCTTTCAACTCAGTCACTCCTGTTTGCTGCAGGCTTGCGACCTACCCCTGCACAAGCAACTGTTGTTAGTTGGGTCCTAAAGGATGGGATGCAGCACATGGGTAAGCTCATATGTAGTAATTTGGGTGCAAGAATGGATTCAGAGCCTAAACGCTGGAGAATTTTGGCTGATGTACTCTATGACTTGGGCACTGGCTTAGAAGTTCTTTCTCCTCTATGTCCACAATTTTTTCTTCAAGTTGCAGGCCTAGGCAATTTTGCAAAGGGGATGGCAGTTGTTGCAGCAAGAGCAACAAGATTACCAATTTATTCCTCCTTTGCAAAAGAAGGCAATCTTAGTGACCTGTTTGCAAAAGGGGAGGCAATCTCAACTGTGTTCAATGTTCTTGGAATAGGGTTAGGAATCCAGTTAGCATCTACTATCTGTTCATCAATTCAAGGAAAGATGGTAGTTGGGCCTCTTCTTTCAATCGTACATGTATATTGTGTCATTGAAGAAATGCGGGCAACTCCTGTGAACACTCTGAATCCTCAGAGGACCGCAATGCTTGTGGCTGATTTCATCAAGACAGGAAAAGTATCAAGCCCAGCTGACCTAAGGTATAAAGAAGATCTTCTCTTTCCTGGGCGACTCATAGAAGATGCTGGAAATGTAAAAGTGGGAAAGTCCTTCCGCAAGGTTTTTAAGCCTTCAAAACTTGATGAATTGAAACAAATATTCCCAGACGAGAAGTTTCTTCTGAATCCTGGAAAAAAATCGGTTGACATGGTATTGGAGCACAGTGCTACCGGTGAAGATGCCTTGAGGGGGTGGCTTGTTGCCGGATTTGCAGCTGACATGGAGAAGTCTTTTCATGTTCCAACTTCAACTGCACTCGAAGAGGCTTACGAGAAGATGAATGATGTATTTGGTCCATTTGTTTCCAAACTTCAGGCCAAGGGGTGGCACACTGATCGTTTTCTTGACGGAACAGGAAGTCGTTTTGCTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gamma-thionin

Protein Analysis

431

Amino Acids

47.65

Weight (kDa)

8.76

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UVB_sens_prot PF04884 59 - 291 3.5e-73 Vitamin B6 photo-protection and homoeostasis domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017253)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g39470
prunus_persica Prupe.6G135400_v2.0.a1
pyrus_communis pycom15g02020
rosa_chinensis RchiOBHm_Chr6g0301481
rosa_laevigata RLG00000011250
rosa_multiflora Rmu_sc0014417.1_g000018
rosa_roxburghii Rroxscaffold_7G00166760
rosa_rugosa Rorug06G0305100
rosa_samantha Rh6AG419100 Rh6BG425000 Rh6CG432300 Rh6DG418200
rosa_wichuraiana Rw6G036240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1245
AciI CCGC 7 cut(s) 53, 56, 59, 109, 805, 843, 976
AclWI GGATC 1 cut(s) 89
AcsI RAATTY 3 cut(s) 116, 147, 462
AcuI CTGAAG 2 cut(s) 114, 1217
AfaI GTAC 4 cut(s) 77, 254, 477, 777
AfeI AGCGCT 1 cut(s) 294
AfiI CCNNNNNNNGG 2 cut(s) 1110, 1245
AflIII ACRYGT 1 cut(s) 778
AgeI ACCGGT 1 cut(s) 1091
AjnI CCWGG 2 cut(s) 925, 1048
AluBI AGCT 4 cut(s) 67, 409, 893, 1136
AluI AGCT 4 cut(s) 67, 409, 893, 1136
Alw21I GWGCWC 1 cut(s) 1083
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 1 cut(s) 89
Ama87I CYCGRG 1 cut(s) 160
Aor51HI AGCGCT 1 cut(s) 294
AoxI GGCC 4 cut(s) 164, 547, 758, 1236
ApeKI GCWGC 6 cut(s) 67, 290, 329, 394, 584, 1133
ApoI RAATTY 3 cut(s) 116, 147, 462
AsiGI ACCGGT 1 cut(s) 1091
Asp700I GAANNNNTTC 2 cut(s) 439, 915
AspA2I CCTAGG 1 cut(s) 550
AspLEI GCGC 2 cut(s) 52, 295
AspS9I GGNCC 4 cut(s) 376, 758, 840, 1214
AsuHPI GGTGA 3 cut(s) 125, 274, 1106
AvaI CYCGRG 1 cut(s) 160
AvaII GGWCC 3 cut(s) 376, 840, 1214
AvrII CCTAGG 1 cut(s) 550
AxyI CCTNAGG 2 cut(s) 214, 899
BaeGI GKGCMC 2 cut(s) 271, 494
Bbv12I GWGCWC 1 cut(s) 1083
BbvI GCAGC 6 cut(s) 54, 302, 316, 406, 596, 1145
BccI CCATC 3 cut(s) 380, 565, 742
BcgI CGANNNNNNTGC 4 cut(s) 279, 313, 1238, 1272
BciT130I CCWGG 2 cut(s) 927, 1050
BcoDI GTCTC 1 cut(s) 106
BfaI CTAG 1 cut(s) 551
BfmI CTRYAG 1 cut(s) 330
BfoI RGCGCY 2 cut(s) 53, 296
BglII AGATCT 1 cut(s) 913
BisI GCNGC 9 cut(s) 53, 56, 59, 68, 291, 330, 395, 585, 1134
BlnI CCTAGG 1 cut(s) 550
BlsI GCNGC 9 cut(s) 54, 57, 60, 69, 292, 331, 396, 586, 1135
Bme1390I CCNGG 2 cut(s) 927, 1050
Bme18I GGWCC 3 cut(s) 376, 840, 1214
BmeT110I CYCGRG 1 cut(s) 160
BmgT120I GGNCC 4 cut(s) 376, 758, 840, 1214
BmiI GGNNCC 2 cut(s) 39, 377
BmrFI CCNGG 2 cut(s) 927, 1050
BmsI GCATC 5 cut(s) 15, 381, 725, 934, 1090
BplI GAGNNNNNCTC 2 cut(s) 653, 685
BpmI CTGGAG 2 cut(s) 32, 478
Bpu10I CCTNAGC 1 cut(s) 63
BpuEI CTTGAG 1 cut(s) 1126
BsaJI CCNNGG 4 cut(s) 127, 550, 926, 1239
BsaWI WCCGGW 1 cut(s) 1091
Bsc4I CCNNNNNNNGG 2 cut(s) 1110, 1245
Bse118I RCCGGY 2 cut(s) 70, 1091
Bse1I ACTGG 6 cut(s) 15, 83, 167, 285, 499, 709
Bse21I CCTNAGG 2 cut(s) 214, 899
Bse3DI GCAATG 1 cut(s) 852
BseBI CCWGG 2 cut(s) 927, 1050
BseDI CCNNGG 4 cut(s) 127, 550, 926, 1239
BseGI GGATG 3 cut(s) 391, 396, 576
BseLI CCNNNNNNNGG 2 cut(s) 1110, 1245
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 5 cut(s) 54, 228, 262, 326, 848
BseNI ACTGG 6 cut(s) 15, 83, 167, 285, 499, 709
BseRI GAGGAG 1 cut(s) 504
BseSI GKGCMC 2 cut(s) 271, 494
BseXI GCAGC 6 cut(s) 54, 302, 316, 406, 596, 1145
BseYI CCCAGC 1 cut(s) 889
BsgI GTGCAG 3 cut(s) 309, 336, 1158
BshFI GGCC 4 cut(s) 166, 549, 760, 1238
BshTI ACCGGT 1 cut(s) 1091
BsiHKAI GWGCWC 1 cut(s) 1083
BsiHKCI CYCGRG 1 cut(s) 160
BsiSI CCGG 3 cut(s) 71, 1092, 1125
BslI CCNNNNNNNGG 2 cut(s) 1110, 1245
BsmAI GTCTC 1 cut(s) 106
BsnI GGCC 4 cut(s) 166, 549, 760, 1238
BsoBI CYCGRG 1 cut(s) 160
Bsp1286I GDGCHC 3 cut(s) 271, 494, 1083
Bsp143I GATC 3 cut(s) 81, 913, 1255
BspACI CCGC 7 cut(s) 53, 56, 59, 109, 805, 843, 976
BspANI GGCC 4 cut(s) 166, 549, 760, 1238
BspCNI CTCAG 5 cut(s) 55, 227, 261, 325, 847
BspLI GGNNCC 2 cut(s) 39, 377
BspMAI CTGCAG 1 cut(s) 334
BspPI GGATC 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 852
BsrFI RCCGGY 2 cut(s) 70, 1091
BsrI ACTGG 6 cut(s) 15, 83, 167, 285, 499, 709
BssAI RCCGGY 2 cut(s) 70, 1091
BssECI CCNNGG 4 cut(s) 127, 550, 926, 1239
BssMI GATC 3 cut(s) 81, 913, 1255
BssT1I CCWWGG 2 cut(s) 550, 1239
Bst2UI CCWGG 2 cut(s) 927, 1050
Bst4CI ACNGT 4 cut(s) 131, 364, 676, 1085
Bst6I CTCTTC 2 cut(s) 768, 1176
BstAPI GCANNNNNTGC 1 cut(s) 581
BstC8I GCNNGC 5 cut(s) 72, 334, 338, 547, 807
BstDEI CTNAG 8 cut(s) 63, 214, 248, 312, 499, 638, 834, 899
BstDSI CCRYGG 1 cut(s) 127
BstEII GGTNACC 1 cut(s) 131
BstF5I GGATG 3 cut(s) 391, 396, 576
BstH2I RGCGCY 2 cut(s) 53, 296
BstHHI GCGC 2 cut(s) 52, 295
BstKTI GATC 3 cut(s) 84, 916, 1258
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 3 cut(s) 81, 913, 1255
BstMWI GCNNNNNNNGC 7 cut(s) 58, 64, 67, 301, 581, 590, 1130
BstNI CCWGG 2 cut(s) 927, 1050
BstNSI RCATGY 1 cut(s) 782
BstPI GGTNACC 1 cut(s) 131
BstSCI CCNGG 2 cut(s) 925, 1048
BstSFI CTRYAG 1 cut(s) 330
BstSLI GKGCMC 2 cut(s) 271, 494
BstV1I GCAGC 6 cut(s) 54, 302, 316, 406, 596, 1145
BstX2I RGATCY 1 cut(s) 913
BstYI RGATCY 1 cut(s) 913
Bsu36I CCTNAGG 2 cut(s) 214, 899
BsuRI GGCC 4 cut(s) 166, 549, 760, 1238
BtgI CCRYGG 1 cut(s) 127
BtsCI GGATG 3 cut(s) 391, 396, 576
BtsI GCAGTG 1 cut(s) 269
BtsIMutI CAGTG 5 cut(s) 269, 292, 492, 1090, 1251
Cac8I GCNNGC 5 cut(s) 72, 334, 338, 547, 807
CfoI GCGC 2 cut(s) 52, 295
Cfr10I RCCGGY 2 cut(s) 70, 1091
Cfr13I GGNCC 4 cut(s) 376, 758, 840, 1214
Csp6I GTAC 4 cut(s) 76, 253, 476, 776
CspAI ACCGGT 1 cut(s) 1091
CspCI CAANNNNNGTGG 2 cut(s) 834, 869
CviAII CATG 5 cut(s) 400, 779, 1069, 1141, 1157
CviQI GTAC 4 cut(s) 76, 253, 476, 776
DdeI CTNAG 8 cut(s) 63, 214, 248, 312, 499, 638, 834, 899
DpnI GATC 3 cut(s) 83, 915, 1257
DpnII GATC 3 cut(s) 81, 913, 1255
Eam1104I CTCTTC 2 cut(s) 768, 1176
EarI CTCTTC 2 cut(s) 768, 1176
Eco130I CCWWGG 2 cut(s) 550, 1239
Eco147I AGGCCT 1 cut(s) 549
Eco32I GATATC 2 cut(s) 221, 245
Eco47I GGWCC 3 cut(s) 376, 840, 1214
Eco47III AGCGCT 1 cut(s) 294
Eco57I CTGAAG 2 cut(s) 114, 1217
Eco81I CCTNAGG 2 cut(s) 214, 899
Eco88I CYCGRG 1 cut(s) 160
Eco91I GGTNACC 1 cut(s) 131
EcoO109I RGGNCCY 1 cut(s) 376
EcoO65I GGTNACC 1 cut(s) 131
EcoRII CCWGG 2 cut(s) 925, 1048
EcoRV GATATC 2 cut(s) 221, 245
EcoT14I CCWWGG 2 cut(s) 550, 1239
ErhI CCWWGG 2 cut(s) 550, 1239
FaeI CATG 5 cut(s) 403, 782, 1072, 1144, 1160
FalI AAGNNNNNCTT 4 cut(s) 621, 653, 900, 932
FatI CATG 5 cut(s) 399, 778, 1068, 1140, 1156
FauI CCCGC 1 cut(s) 798
FauNDI CATATG 1 cut(s) 413
Fnu4HI GCNGC 9 cut(s) 53, 56, 59, 68, 291, 330, 395, 585, 1134
FokI GGATG 3 cut(s) 398, 403, 583
Fsp4HI GCNGC 9 cut(s) 53, 56, 59, 68, 291, 330, 395, 585, 1134
FspBI CTAG 1 cut(s) 551
GlaI GCGC 2 cut(s) 51, 294
GluI GCNGC 9 cut(s) 53, 56, 59, 68, 291, 330, 395, 585, 1134
GsaI CCCAGC 1 cut(s) 893
GsuI CTGGAG 2 cut(s) 32, 478
HaeII RGCGCY 2 cut(s) 53, 296
HaeIII GGCC 4 cut(s) 166, 549, 760, 1238
HapII CCGG 3 cut(s) 71, 1092, 1125
HhaI GCGC 2 cut(s) 52, 295
Hin1II CATG 5 cut(s) 403, 782, 1072, 1144, 1160
Hin6I GCGC 2 cut(s) 50, 293
HinP1I GCGC 2 cut(s) 50, 293
HincII GTYRAC 1 cut(s) 1066
HindII GTYRAC 1 cut(s) 1066
HinfI GANTC 7 cut(s) 158, 188, 440, 705, 829, 933, 1045
HpaII CCGG 3 cut(s) 71, 1092, 1125
HphI GGTGA 3 cut(s) 125, 274, 1106
Hpy166II GTNNAC 4 cut(s) 255, 524, 820, 1066
Hpy188I TCNGA 5 cut(s) 94, 445, 828, 837, 1044
Hpy188III TCNNGA 4 cut(s) 152, 216, 868, 1265
Hpy8I GTNNAC 4 cut(s) 255, 524, 820, 1066
HpyAV CCTTC 5 cut(s) 28, 202, 623, 982, 1002
HpyCH4III ACNGT 4 cut(s) 131, 364, 676, 1085
HpyF10VI GCNNNNNNNGC 7 cut(s) 58, 64, 67, 301, 581, 590, 1130
HpyF3I CTNAG 8 cut(s) 63, 214, 248, 312, 499, 638, 834, 899
Hsp92II CATG 5 cut(s) 403, 782, 1072, 1144, 1160
HspAI GCGC 2 cut(s) 50, 293
KroI GCCGGC 1 cut(s) 70
KroNI GCCGGC 1 cut(s) 72
Kzo9I GATC 3 cut(s) 81, 913, 1255
LmnI GCTCC 2 cut(s) 37, 1078
Lsp1109I GCAGC 6 cut(s) 54, 302, 316, 406, 596, 1145
LweI GCATC 5 cut(s) 15, 381, 725, 934, 1090
MaeI CTAG 1 cut(s) 551
MaeIII GTNAC 3 cut(s) 131, 315, 641
MalI GATC 3 cut(s) 83, 915, 1257
MboI GATC 3 cut(s) 81, 913, 1255
MflI RGATCY 1 cut(s) 913
MhlI GDGCHC 3 cut(s) 271, 494, 1083
MlyI GAGTC 2 cut(s) 152, 927
MmeI TCCRAC 1 cut(s) 1187
MroNI GCCGGC 1 cut(s) 70
MroXI GAANNNNTTC 2 cut(s) 439, 915
MseI TTAA 1 cut(s) 987
MspA1I CMGCKG 3 cut(s) 111, 893, 1136
MspI CCGG 3 cut(s) 71, 1092, 1125
MspR9I CCNGG 2 cut(s) 927, 1050
MvaI CCWGG 2 cut(s) 927, 1050
MwoI GCNNNNNNNGC 7 cut(s) 58, 64, 67, 301, 581, 590, 1130
NaeI GCCGGC 1 cut(s) 72
NdeI CATATG 1 cut(s) 413
NdeII GATC 3 cut(s) 81, 913, 1255
NgoMIV GCCGGC 1 cut(s) 70
NlaIII CATG 5 cut(s) 403, 782, 1072, 1144, 1160
NlaIV GGNNCC 2 cut(s) 39, 377
NmuCI GTSAC 3 cut(s) 131, 315, 641
NspI RCATGY 1 cut(s) 782
PaeR7I CTCGAG 1 cut(s) 160
PceI AGGCCT 1 cut(s) 549
PciI ACATGT 1 cut(s) 778
PdiI GCCGGC 1 cut(s) 72
PdmI GAANNNNTTC 2 cut(s) 439, 915
PfeI GAWTC 5 cut(s) 188, 440, 705, 829, 1045
PflMI CCANNNNNTGG 1 cut(s) 1245
PfoI TCCNGGA 1 cut(s) 1048
PinAI ACCGGT 1 cut(s) 1091
PkrI GCNGC 9 cut(s) 54, 57, 60, 69, 292, 331, 396, 586, 1135
PleI GAGTC 2 cut(s) 152, 927
PpsI GAGTC 2 cut(s) 152, 927
PpuMI RGGWCCY 1 cut(s) 376
PscI ACATGT 1 cut(s) 778
Psp5II RGGWCCY 1 cut(s) 376
Psp6I CCWGG 2 cut(s) 925, 1048
PspEI GGTNACC 1 cut(s) 131
PspFI CCCAGC 1 cut(s) 889
PspGI CCWGG 2 cut(s) 925, 1048
PspN4I GGNNCC 2 cut(s) 39, 377
PspPI GGNCC 4 cut(s) 376, 758, 840, 1214
PspPPI RGGWCCY 1 cut(s) 376
PspXI VCTCGAGB 1 cut(s) 160
PstI CTGCAG 1 cut(s) 334
PsuI RGATCY 1 cut(s) 913
PvuII CAGCTG 2 cut(s) 893, 1136
RsaI GTAC 4 cut(s) 77, 254, 477, 777
RsaNI GTAC 4 cut(s) 76, 253, 476, 776
SaqAI TTAA 1 cut(s) 987
SatI GCNGC 9 cut(s) 53, 56, 59, 68, 291, 330, 395, 585, 1134
Sau3AI GATC 3 cut(s) 81, 913, 1255
Sau96I GGNCC 4 cut(s) 376, 758, 840, 1214
SchI GAGTC 2 cut(s) 152, 927
ScrFI CCNGG 2 cut(s) 927, 1050
SduI GDGCHC 3 cut(s) 271, 494, 1083
SfaNI GCATC 5 cut(s) 15, 381, 725, 934, 1090
SfcI CTRYAG 1 cut(s) 330
Sfr274I CTCGAG 1 cut(s) 160
SinI GGWCC 3 cut(s) 376, 840, 1214
SlaI CTCGAG 1 cut(s) 160
SmlI CTYRAG 2 cut(s) 160, 1105
SmoI CTYRAG 2 cut(s) 160, 1105
SseBI AGGCCT 1 cut(s) 549
SsiI CCGC 7 cut(s) 53, 56, 59, 109, 805, 843, 976
SspI AATATT 1 cut(s) 1020
SspMI CTAG 1 cut(s) 551
StuI AGGCCT 1 cut(s) 549
StyD4I CCNGG 2 cut(s) 925, 1048
StyI CCWWGG 2 cut(s) 550, 1239
TaaI ACNGT 4 cut(s) 131, 364, 676, 1085
TaqI TCGA 3 cut(s) 84, 161, 1179
TatI WGTACW 1 cut(s) 475
TauI GCSGC 3 cut(s) 55, 58, 61
TfiI GAWTC 5 cut(s) 188, 440, 705, 829, 1045
Tru1I TTAA 1 cut(s) 987
Tru9I TTAA 1 cut(s) 987
TscAI CASTG 5 cut(s) 276, 292, 499, 1090, 1258
TseFI GTSAC 3 cut(s) 131, 315, 641
TseI GCWGC 6 cut(s) 67, 290, 329, 394, 584, 1133
Tsp45I GTSAC 3 cut(s) 131, 315, 641
TspDTI ATGAA 6 cut(s) 17, 720, 853, 1020, 1145, 1214
TspGWI ACGGA 2 cut(s) 164, 1284
TspRI CASTG 5 cut(s) 276, 292, 499, 1090, 1258
Van91I CCANNNNNTGG 1 cut(s) 1245
VpaK11BI GGWCC 3 cut(s) 376, 840, 1214
XapI RAATTY 3 cut(s) 116, 147, 462
XceI RCATGY 1 cut(s) 782
XhoI CTCGAG 1 cut(s) 160
XmaJI CCTAGG 1 cut(s) 550
XmnI GAANNNNTTC 2 cut(s) 439, 915
XspI CTAG 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.