Rorug06G0322200

expansin-A11-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0014412
Physical Location & Seq
Forward (+)
47398769 .. 47401210
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0322200.1

Sequence Viewer

Length: 1203 bp
ATGGCGCCTCCCAAGAAATCAAAGAAAAATAAGAAAGATGGCAAATTGAAGAAATGCAAAAGCTCAAGTGTGGCTCCAGTCGAGCCTACAGCTCCTGACTCTGAGTGGTGGGATAGTTTCTGGCACAAGAATGCTTCAAACCCAGGCTCTTCACTATCGAAAGATGAGGAAGAAGGATTTAAGTATTTCTTTAGGGTCTCTAGAAAGACTTTTGATTACATCTGTTCTATTGTAAGAGAAGACCTTGTGTCGCGGCCACCATCAGGGCTCATCAACATTGAAGGGAGGCTTCTTAGTGTTGAGAAACAGGTTGCAATTGCCATGAGAAGGTTGGCATCTGGTGAGTCTCAGGTCTCAGTGGGAGCCGCCTTTGGGGTCGGCCAGTCCACAGTTTCTCAGGTGACTTGGAGATTCATTGAAGCATTGGAAGAACGTGCCAAGCACCATCTCAAGTGGCCTGATTCCAATAGGATGGAGGAAATTAAGTCTAGACTTGAAGCATCTTTTGGATTGCCAAATTGCTGTGGAGCCATAGACGGCACACACATCATAATGACACTTCCTACTGTTCAAGCATCAGACGATTGGTGTGACACTGAGAAAAATTACAGCATGCTCTTGCAGGGAACTGTCGATCATGAGTTAAGATTCCTTGATATTGTGACCGGTTGGCCTGGGGGAATGACAGTGTCTAGACTATTAAAGTGTTCAGGACTTTTCAAGCTGTGCGAAAGTGGACAGCGTTTAAATGGGAATGTTAGAACTTTATCTGAAGGAGGAGAGATCAGAGAATATGTGGTTGGTGGGGTTGGTTATCCTCTCCTTCCTTGGCTCATAACTCCTCATGAAAGCAGTGGCCTCCCGGCTTCTGTTGTTTCTGCTTTTAATGCCACGCATGAGGCTGCAAGGTCTCTTGCAGTAAGGGCATTCTCGCAGTTAAAGGGCACTTGGAGAATCCTCAGCAAGGTTATGTGGAGACCAGATAAACGGAAACTTCCAAGCATTATCTTGGTATGTTGTTTACTCCACAATATAATAATCGACTGTGGAGATATATTAAACCCAGATGTTGCTTTATCTGGTCATCACGACTCGGGATATGGAGAACAATGCTGTAAGCAAGTTGATCCATTGGGGAGGTCTATGAGGGAGAACTTAGTTAGACACTGGCAGCATAGCAAGCGAACAGCTGCATCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0016020 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

400

Amino Acids

44.46

Weight (kDa)

8.92

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_tran PF04827 153 - 348 1.1e-07 Plant transposon protein
DDE_Tnp_4 PF13359 178 - 344 3.7e-18 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20190 AT1G20190
fragaria_vesca FvH4_2g38040
malus_domestica MD03G1275400.v1.1 MD08G1009000.v1.1 MD15G1007700.v1.1
prunus_persica Prupe.1G360700_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0303551
rosa_laevigata RLG00000011074
rosa_multiflora Rmu_ssc0000114.1_g000030
rosa_roxburghii Rroxscaffold_7G00164640
rosa_rugosa Rorug06G0322200
rosa_samantha Rh6AG436700 Rh6BG466700 Rh6CG448600 Rh6DG435400
rosa_wichuraiana Rw6G037790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 1 cut(s) 253
AciI CCGC 2 cut(s) 253, 366
AclWI GGATC 1 cut(s) 1121
AcoI YGGCCR 2 cut(s) 254, 379
AcuI CTGAAG 1 cut(s) 792
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 4 cut(s) 263, 327, 372, 964
AgeI ACCGGT 1 cut(s) 665
AgsI TTSAA 7 cut(s) 49, 138, 281, 419, 497, 572, 721
AhdI GACNNNNNGTC 1 cut(s) 247
AjnI CCWGG 2 cut(s) 142, 673
AjuI GAANNNNNNNTTGG 4 cut(s) 489, 521, 783, 815
AluBI AGCT 4 cut(s) 63, 92, 724, 1190
AluI AGCT 4 cut(s) 63, 92, 724, 1190
Alw26I GTCTC 5 cut(s) 202, 351, 358, 915, 970
AlwI GGATC 1 cut(s) 1121
AlwNI CAGNNNCTG 1 cut(s) 95
Ama87I CYCGRG 1 cut(s) 1093
AoxI GGCC 5 cut(s) 254, 379, 455, 671, 856
ApeKI GCWGC 3 cut(s) 902, 1171, 1190
AsiGI ACCGGT 1 cut(s) 665
AspLEI GCGC 1 cut(s) 7
AsuC2I CCSGG 1 cut(s) 863
AsuHPI GGTGA 2 cut(s) 353, 412
AvaI CYCGRG 1 cut(s) 1093
BaeGI GKGCMC 1 cut(s) 947
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 246
BbvCI CCTCAGC 1 cut(s) 959
BbvI GCAGC 3 cut(s) 889, 1177, 1183
BccI CCATC 4 cut(s) 32, 268, 453, 466
BceAI ACGGC 1 cut(s) 553
BciT130I CCWGG 2 cut(s) 144, 675
BcnI CCSGG 1 cut(s) 863
BcoDI GTCTC 5 cut(s) 202, 351, 358, 915, 970
BfaI CTAG 3 cut(s) 201, 489, 693
BfmI CTRYAG 1 cut(s) 87
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 5 cut(s) 254, 366, 903, 1172, 1191
BlsI GCNGC 5 cut(s) 255, 367, 904, 1173, 1192
Bme1390I CCNGG 3 cut(s) 144, 675, 863
BmeRI GACNNNNNGTC 1 cut(s) 247
BmeT110I CYCGRG 1 cut(s) 1093
BmiI GGNNCC 4 cut(s) 6, 75, 364, 529
BmrFI CCNGG 3 cut(s) 144, 675, 863
BmsI GCATC 3 cut(s) 344, 509, 584
BpiI GAAGAC 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 60
Bpu10I CCTNAGC 1 cut(s) 959
BpuEI CTTGAG 2 cut(s) 49, 434
BpuMI CCSGG 1 cut(s) 863
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 4 cut(s) 202, 358, 915, 970
BsaJI CCNNGG 3 cut(s) 142, 674, 827
BsaWI WCCGGW 1 cut(s) 665
Bsc4I CCNNNNNNNGG 4 cut(s) 263, 327, 372, 964
Bse118I RCCGGY 1 cut(s) 665
Bse1I ACTGG 3 cut(s) 77, 382, 1172
BseBI CCWGG 2 cut(s) 144, 675
BseDI CCNNGG 3 cut(s) 142, 674, 827
BseGI GGATG 1 cut(s) 477
BseLI CCNNNNNNNGG 4 cut(s) 263, 327, 372, 964
BseMII CTCAG 6 cut(s) 93, 362, 369, 410, 588, 973
BseNI ACTGG 3 cut(s) 77, 382, 1172
BseRI GAGGAG 2 cut(s) 792, 831
BseSI GKGCMC 1 cut(s) 947
BseXI GCAGC 3 cut(s) 889, 1177, 1183
Bsh1236I CGCG 1 cut(s) 253
BshFI GGCC 5 cut(s) 256, 381, 457, 673, 858
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 665
BsiHKCI CYCGRG 1 cut(s) 1093
BsiSI CCGG 2 cut(s) 666, 863
BslI CCNNNNNNNGG 4 cut(s) 263, 327, 372, 964
BsmAI GTCTC 5 cut(s) 202, 351, 358, 915, 970
BsmI GAATGC 2 cut(s) 136, 926
BsnI GGCC 5 cut(s) 256, 381, 457, 673, 858
Bso31I GGTCTC 4 cut(s) 202, 358, 915, 970
BsoBI CYCGRG 1 cut(s) 1093
Bsp1286I GDGCHC 2 cut(s) 270, 947
Bsp143I GATC 3 cut(s) 634, 783, 1126
BspACI CCGC 2 cut(s) 253, 366
BspANI GGCC 5 cut(s) 256, 381, 457, 673, 858
BspCNI CTCAG 6 cut(s) 94, 361, 368, 409, 589, 972
BspFNI CGCG 1 cut(s) 253
BspHI TCATGA 2 cut(s) 637, 844
BspLI GGNNCC 4 cut(s) 6, 75, 364, 529
BspPI GGATC 1 cut(s) 1121
BspQI GCTCTTC 1 cut(s) 154
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 4 cut(s) 202, 358, 915, 970
BsrFI RCCGGY 1 cut(s) 665
BsrI ACTGG 3 cut(s) 77, 382, 1172
BssAI RCCGGY 1 cut(s) 665
BssECI CCNNGG 3 cut(s) 142, 674, 827
BssMI GATC 3 cut(s) 634, 783, 1126
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 827
Bst2UI CCWGG 2 cut(s) 144, 675
Bst4CI ACNGT 5 cut(s) 391, 568, 631, 688, 1046
Bst6I CTCTTC 1 cut(s) 154
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 2 cut(s) 614, 1181
BstDEI CTNAG 8 cut(s) 102, 293, 348, 355, 396, 597, 959, 1156
BstENI CCTNNNNNAGG 1 cut(s) 962
BstF5I GGATG 1 cut(s) 477
BstFNI CGCG 1 cut(s) 253
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 3 cut(s) 637, 786, 1129
BstMAI GTCTC 5 cut(s) 202, 351, 358, 915, 970
BstMBI GATC 3 cut(s) 634, 783, 1126
BstMWI GCNNNNNNNGC 3 cut(s) 887, 923, 1180
BstNI CCWGG 2 cut(s) 144, 675
BstNSI RCATGY 1 cut(s) 616
BstSCI CCNGG 3 cut(s) 142, 673, 861
BstSFI CTRYAG 1 cut(s) 87
BstSLI GKGCMC 1 cut(s) 947
BstUI CGCG 1 cut(s) 253
BstV1I GCAGC 3 cut(s) 889, 1177, 1183
BstV2I GAAGAC 1 cut(s) 246
BstXI CCANNNNNNTGG 1 cut(s) 472
BsuRI GGCC 5 cut(s) 256, 381, 457, 673, 858
BtsCI GGATG 1 cut(s) 477
BtsI GCAGTG 1 cut(s) 859
BtsIMutI CAGTG 5 cut(s) 363, 594, 693, 859, 1165
Cac8I GCNNGC 2 cut(s) 614, 1181
CaiI CAGNNNCTG 1 cut(s) 95
CciI TCATGA 2 cut(s) 637, 844
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 665
CspAI ACCGGT 1 cut(s) 665
CviAII CATG 5 cut(s) 322, 613, 638, 845, 896
DdeI CTNAG 8 cut(s) 102, 293, 348, 355, 396, 597, 959, 1156
DinI GGCGCC 1 cut(s) 6
DpnI GATC 3 cut(s) 636, 785, 1128
DpnII GATC 3 cut(s) 634, 783, 1126
DraI TTTAAA 1 cut(s) 747
DriI GACNNNNNGTC 1 cut(s) 247
EaeI YGGCCR 2 cut(s) 254, 379
Eam1104I CTCTTC 1 cut(s) 154
Eam1105I GACNNNNNGTC 1 cut(s) 247
EarI CTCTTC 1 cut(s) 154
Eco130I CCWWGG 1 cut(s) 827
Eco24I GRGCYC 1 cut(s) 270
Eco31I GGTCTC 4 cut(s) 202, 358, 915, 970
Eco57I CTGAAG 1 cut(s) 792
Eco88I CYCGRG 1 cut(s) 1093
EcoNI CCTNNNNNAGG 1 cut(s) 962
EcoRII CCWGG 2 cut(s) 142, 673
EcoT14I CCWWGG 1 cut(s) 827
EcoT38I GRGCYC 1 cut(s) 270
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 827
FaeI CATG 5 cut(s) 325, 616, 641, 848, 899
FalI AAGNNNNNCTT 4 cut(s) 173, 205, 273, 305
FatI CATG 5 cut(s) 321, 612, 637, 844, 895
Fnu4HI GCNGC 5 cut(s) 254, 366, 903, 1172, 1191
FokI GGATG 1 cut(s) 484
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 5 cut(s) 254, 366, 903, 1172, 1191
FspBI CTAG 3 cut(s) 201, 489, 693
GlaI GCGC 1 cut(s) 6
GluI GCNGC 5 cut(s) 254, 366, 903, 1172, 1191
GsuI CTGGAG 1 cut(s) 60
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 5 cut(s) 256, 381, 457, 673, 858
HapII CCGG 2 cut(s) 666, 863
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 325, 616, 641, 848, 899
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 7 cut(s) 98, 344, 411, 461, 648, 954, 1091
HpaII CCGG 2 cut(s) 666, 863
HphI GGTGA 2 cut(s) 353, 412
Hpy166II GTNNAC 3 cut(s) 387, 737, 1022
Hpy188I TCNGA 4 cut(s) 103, 580, 772, 788
Hpy188III TCNNGA 9 cut(s) 95, 201, 489, 638, 693, 711, 845, 1088, 1095
Hpy8I GTNNAC 3 cut(s) 387, 737, 1022
HpyAV CCTTC 5 cut(s) 167, 275, 321, 767, 833
HpyCH4III ACNGT 5 cut(s) 391, 568, 631, 688, 1046
HpyCH4IV ACGT 1 cut(s) 433
HpyCH4V TGCA 6 cut(s) 57, 314, 622, 905, 917, 1193
HpyF10VI GCNNNNNNNGC 3 cut(s) 887, 923, 1180
HpyF3I CTNAG 8 cut(s) 102, 293, 348, 355, 396, 597, 959, 1156
HpySE526I ACGT 1 cut(s) 433
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 325, 616, 641, 848, 899
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 3 cut(s) 634, 783, 1126
LguI GCTCTTC 1 cut(s) 154
LmnI GCTCC 4 cut(s) 79, 97, 362, 527
Lsp1109I GCAGC 3 cut(s) 889, 1177, 1183
LweI GCATC 3 cut(s) 344, 509, 584
MaeI CTAG 3 cut(s) 201, 489, 693
MaeII ACGT 1 cut(s) 433
MaeIII GTNAC 3 cut(s) 400, 590, 661
MalI GATC 3 cut(s) 636, 785, 1128
MboI GATC 3 cut(s) 634, 783, 1126
MboII GAAGA 5 cut(s) 61, 141, 182, 251, 440
MfeI CAATTG 1 cut(s) 315
MhlI GDGCHC 2 cut(s) 270, 947
MluCI AATT 5 cut(s) 44, 315, 480, 517, 604
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 3 cut(s) 92, 353, 1085
MseI TTAA 8 cut(s) 180, 483, 644, 701, 746, 885, 938, 1058
MslI CAYNNNNRTG 3 cut(s) 129, 551, 1198
MspA1I CMGCKG 1 cut(s) 1190
MspI CCGG 2 cut(s) 666, 863
MspR9I CCNGG 3 cut(s) 144, 675, 863
MunI CAATTG 1 cut(s) 315
Mva1269I GAATGC 2 cut(s) 136, 926
MvaI CCWGG 2 cut(s) 144, 675
MvnI CGCG 1 cut(s) 253
MwoI GCNNNNNNNGC 3 cut(s) 887, 923, 1180
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 863
NdeII GATC 3 cut(s) 634, 783, 1126
NlaIII CATG 5 cut(s) 325, 616, 641, 848, 899
NlaIV GGNNCC 4 cut(s) 6, 75, 364, 529
NmuCI GTSAC 3 cut(s) 400, 590, 661
NspI RCATGY 1 cut(s) 616
PaeI GCATGC 1 cut(s) 616
PagI TCATGA 2 cut(s) 637, 844
PciSI GCTCTTC 1 cut(s) 154
PctI GAATGC 2 cut(s) 136, 926
PfeI GAWTC 4 cut(s) 411, 461, 648, 954
PflFI GACNNNGTC 1 cut(s) 688
PinAI ACCGGT 1 cut(s) 665
PkrI GCNGC 5 cut(s) 255, 367, 904, 1173, 1192
PleI GAGTC 3 cut(s) 92, 352, 1085
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 3 cut(s) 92, 352, 1085
Psp6I CCWGG 2 cut(s) 142, 673
PspGI CCWGG 2 cut(s) 142, 673
PspN4I GGNNCC 4 cut(s) 6, 75, 364, 529
PstNI CAGNNNCTG 1 cut(s) 95
PsyI GACNNNGTC 1 cut(s) 688
PvuII CAGCTG 1 cut(s) 1190
RseI CAYNNNNRTG 3 cut(s) 129, 551, 1198
SapI GCTCTTC 1 cut(s) 154
SaqAI TTAA 8 cut(s) 180, 483, 644, 701, 746, 885, 938, 1058
SatI GCNGC 5 cut(s) 254, 366, 903, 1172, 1191
Sau3AI GATC 3 cut(s) 634, 783, 1126
SchI GAGTC 3 cut(s) 92, 353, 1085
ScrFI CCNGG 3 cut(s) 144, 675, 863
SduI GDGCHC 2 cut(s) 270, 947
SfaNI GCATC 3 cut(s) 344, 509, 584
SfcI CTRYAG 1 cut(s) 87
SfoI GGCGCC 1 cut(s) 6
SmiMI CAYNNNNRTG 3 cut(s) 129, 551, 1198
SmlI CTYRAG 2 cut(s) 64, 449
SmoI CTYRAG 2 cut(s) 64, 449
SphI GCATGC 1 cut(s) 616
Sse9I AATT 5 cut(s) 44, 315, 480, 517, 604
SsiI CCGC 2 cut(s) 253, 366
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 3 cut(s) 201, 489, 693
StyD4I CCNGG 3 cut(s) 142, 673, 861
StyI CCWWGG 1 cut(s) 827
TaaI ACNGT 5 cut(s) 391, 568, 631, 688, 1046
TaiI ACGT 1 cut(s) 436
TaqI TCGA 4 cut(s) 81, 158, 633, 1041
TasI AATT 5 cut(s) 44, 315, 480, 517, 604
TauI GCSGC 2 cut(s) 256, 368
TfiI GAWTC 4 cut(s) 411, 461, 648, 954
Tru1I TTAA 8 cut(s) 180, 483, 644, 701, 746, 885, 938, 1058
Tru9I TTAA 8 cut(s) 180, 483, 644, 701, 746, 885, 938, 1058
TscAI CASTG 5 cut(s) 363, 601, 693, 859, 1172
TseFI GTSAC 3 cut(s) 400, 590, 661
TseI GCWGC 3 cut(s) 902, 1171, 1190
Tsp45I GTSAC 3 cut(s) 400, 590, 661
TspDTI ATGAA 2 cut(s) 403, 861
TspGWI ACGGA 1 cut(s) 1003
TspRI CASTG 5 cut(s) 363, 601, 693, 859, 1172
Tth111I GACNNNGTC 1 cut(s) 688
XagI CCTNNNNNAGG 1 cut(s) 962
XbaI TCTAGA 3 cut(s) 200, 488, 692
XceI RCATGY 1 cut(s) 616
XcmI CCANNNNNNNNNTGG 1 cut(s) 328
XspI CTAG 3 cut(s) 201, 489, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.