Rorug06G0333200

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007668
Physical Location & Seq
Forward (+)
48602012 .. 48602383
372 bp
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UTR
Exon/CDS
Intron
Rorug06G0333200.1

Sequence Viewer

Length: 372 bp
ATGATTATTAGGTATGTGGCGTATGATGGTGGAAAACGTTCGACGGTGGGATTCTTGGTTTTCGAATTGAATTTTGACAAGCGTGAATGGGTTGCCAAAGACAACTTAGGTGATGATGTTGCGCTCTTTGTGGGTGATAGTTCTTCTGTAACTGTGCCGGCTTCAAAATCAGGATGTCTATCAAATTGCATATACTTTATCCATGATAATGATGGTCAGGGCTATGATATTGATCGACGCTATCATTTAGATTTTGGTGTTTACAATGTCAAAAGTAAAAGCATTTCACAACCTTACACCTCGCATGCCAAGAGCCTCGTGGAAATGACTGATTATGAACCAGTTTGGGTCAAACCAACTTTAAATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UCH zf-MYND

Protein Analysis

123

Amino Acids

13.9

Weight (kDa)

5.49

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 12 - 89 1.8e-15 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 37
AcsI RAATTY 2 cut(s) 70, 364
AgsI TTSAA 2 cut(s) 70, 165
ApoI RAATTY 2 cut(s) 70, 364
Asp700I GAANNNNTTC 1 cut(s) 37
AspLEI GCGC 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 122, 146
AsuII TTCGAA 1 cut(s) 63
BauI CACGAG 1 cut(s) 317
BccI CCATC 2 cut(s) 20, 206
Bpu14I TTCGAA 1 cut(s) 63
BsaBI GATNNNNATC 2 cut(s) 178, 231
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 341
Bse8I GATNNNNATC 2 cut(s) 178, 231
BseGI GGATG 1 cut(s) 179
BseJI GATNNNNATC 2 cut(s) 178, 231
BseNI ACTGG 1 cut(s) 341
BsiSI CCGG 1 cut(s) 158
Bsp119I TTCGAA 1 cut(s) 63
Bsp143I GATC 1 cut(s) 232
BspT104I TTCGAA 1 cut(s) 63
BsrFI RCCGGY 1 cut(s) 157
BsrI ACTGG 1 cut(s) 341
BssAI RCCGGY 1 cut(s) 157
BssMI GATC 1 cut(s) 232
BssSI CACGAG 1 cut(s) 317
Bst2BI CACGAG 1 cut(s) 317
Bst4CI ACNGT 2 cut(s) 46, 154
BstBI TTCGAA 1 cut(s) 63
BstC8I GCNNGC 2 cut(s) 159, 306
BstDEI CTNAG 1 cut(s) 106
BstF5I GGATG 1 cut(s) 179
BstHHI GCGC 1 cut(s) 124
BstKTI GATC 1 cut(s) 235
BstMBI GATC 1 cut(s) 232
BstNSI RCATGY 1 cut(s) 308
BtsCI GGATG 1 cut(s) 179
Cac8I GCNNGC 2 cut(s) 159, 306
CfoI GCGC 1 cut(s) 124
Cfr10I RCCGGY 1 cut(s) 157
CseI GACGC 1 cut(s) 246
CviAII CATG 2 cut(s) 203, 305
CviJI RGCY 3 cut(s) 161, 222, 315
CviKI_1 RGCY 3 cut(s) 161, 222, 315
DdeI CTNAG 1 cut(s) 106
DpnI GATC 1 cut(s) 234
DpnII GATC 1 cut(s) 232
DraI TTTAAA 1 cut(s) 363
FaeI CATG 2 cut(s) 206, 308
FaiI YATR 8 cut(s) 15, 24, 191, 193, 204, 225, 306, 336
FatI CATG 2 cut(s) 202, 304
FokI GGATG 1 cut(s) 186
GlaI GCGC 1 cut(s) 123
HapII CCGG 1 cut(s) 158
HgaI GACGC 1 cut(s) 246
HhaI GCGC 1 cut(s) 124
Hin1II CATG 2 cut(s) 206, 308
Hin6I GCGC 1 cut(s) 122
HinP1I GCGC 1 cut(s) 122
HinfI GANTC 1 cut(s) 51
HpaII CCGG 1 cut(s) 158
HphI GGTGA 2 cut(s) 122, 146
Hpy166II GTNNAC 1 cut(s) 262
Hpy188III TCNNGA 1 cut(s) 171
Hpy8I GTNNAC 1 cut(s) 262
Hpy99I CGWCG 2 cut(s) 46, 240
HpyCH4III ACNGT 2 cut(s) 46, 154
HpyCH4IV ACGT 1 cut(s) 37
HpyCH4V TGCA 1 cut(s) 189
HpyF3I CTNAG 1 cut(s) 106
HpySE526I ACGT 1 cut(s) 37
Hsp92II CATG 2 cut(s) 206, 308
HspAI GCGC 1 cut(s) 122
KroI GCCGGC 1 cut(s) 157
KroNI GCCGGC 1 cut(s) 159
Kzo9I GATC 1 cut(s) 232
LpnPI CCDG 4 cut(s) 156, 171, 203, 354
MaeII ACGT 1 cut(s) 37
MaeIII GTNAC 1 cut(s) 148
MalI GATC 1 cut(s) 234
MboI GATC 1 cut(s) 232
MboII GAAGA 1 cut(s) 135
MluCI AATT 4 cut(s) 65, 70, 184, 364
MnlI CCTC 2 cut(s) 310, 326
MroNI GCCGGC 1 cut(s) 157
MroXI GAANNNNTTC 1 cut(s) 37
MseI TTAA 1 cut(s) 362
MslI CAYNNNNRTG 1 cut(s) 207
MspI CCGG 1 cut(s) 158
NaeI GCCGGC 1 cut(s) 159
NdeII GATC 1 cut(s) 232
NgoMIV GCCGGC 1 cut(s) 157
NlaIII CATG 2 cut(s) 206, 308
NspI RCATGY 1 cut(s) 308
NspV TTCGAA 1 cut(s) 63
PaeI GCATGC 1 cut(s) 308
PdiI GCCGGC 1 cut(s) 159
PdmI GAANNNNTTC 1 cut(s) 37
PfeI GAWTC 1 cut(s) 51
Psp1406I AACGTT 1 cut(s) 37
RseI CAYNNNNRTG 1 cut(s) 207
SaqAI TTAA 1 cut(s) 362
Sau3AI GATC 1 cut(s) 232
SetI ASST 5 cut(s) 14, 40, 112, 295, 302
SfuI TTCGAA 1 cut(s) 63
SmiMI CAYNNNNRTG 1 cut(s) 207
SphI GCATGC 1 cut(s) 308
Sse9I AATT 4 cut(s) 65, 70, 184, 364
TaaI ACNGT 2 cut(s) 46, 154
TaiI ACGT 1 cut(s) 40
TaqI TCGA 3 cut(s) 41, 63, 235
TasI AATT 4 cut(s) 65, 70, 184, 364
TfiI GAWTC 1 cut(s) 51
Tru1I TTAA 1 cut(s) 362
Tru9I TTAA 1 cut(s) 362
TspDTI ATGAA 1 cut(s) 351
XapI RAATTY 2 cut(s) 70, 364
XceI RCATGY 1 cut(s) 308
XcmI CCANNNNNNNNNTGG 2 cut(s) 209, 316
XmnI GAANNNNTTC 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.