Rorug07G0062800

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008923
Physical Location & Seq
Forward (+)
4758709 .. 4766278
7570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0062800.1

Sequence Viewer

Length: 1860 bp
ATGGCGGCAGAAAAGCGGCTTATGGTGGTCGTCGACGGAACAACAGCTATGGGGCCTTACTGGAAATCCATTCTCTCAGATTACCTCGAAAAGATCATCAGGAACATAAGTGGCCAACCGTCAGCTAACCAGGATGCTTCAGCTGGTGCAGCAGTATACCTGGGCCTGGTTCAGTATAATTCTCACGGACTTGGTAACACTGGTTCCTTAACGCGACCAAGTCCTTGGACTAGAAATGTGGATGGGTTTCTTGAAGGGCTCTCGGCTTTACATTTTTCTGGTGGTGGTGGTCCTAATGAAGCCGCCATTGCTGGGGGGCTGGCAGAAGCACTTGTGATGTTTCCGAAGCCTACTCCCACTGGAACTGGAACTGGAACTGGAACTGATCAAGTTCAAGACCATAATGCCAGAGAAAGGCATTGTATTCTTGTGGCTGCAAGCAACCCCGTGTTGTTTCGAACCGCCGTTCAGATACCGATAATAAGTAATGGCCAATTTTCTTCTGGTACACAAACTCGATGGACTCTTGCTGATGCTCAAGATGTTGCCAAATTATATGCTGAGGGATATATTGATGATCCTGAAGGAACACCTTCCTTCAGCGATGCGAACAATCCTGACGTACTTCTTCTTTTGTCGAAGGACTTCTTAGAGGCTCATGCTGCCCTTATTCAGTATGTAGAAGTGAACATACTGTCAGCTCCGGAGATAAGTCAGATAACAGACTCTGAGCTTGAATATATTGCTTTAGTCCATTTCGGAGACAACAGTGAGTTTGATTATATAGGAGATATGTTGGATTCACCCGAGACAGATAAGCAAGATCATCGTGCAGAGAACATTCTCAATGAAACCGAAGAAATGCTTCTTGCAGCAACACAAGACAGTTTTTCACAAGCACCTTCACCCACCTGTTTCATTAGTGAGACATTGTCCAGTAGCCTTATTCAGTACCCCGAAGTACAAGCAAGGGGATCTGAAGCCGGAGAAAGTACTGCTAGTACTACGCAAGCAGAACTCCACTCTCCACAGCAGGCGCAACAAATACTTGCAACTCGTACCATAAGTGGAGTACACAGATCGCAAGGATCTTGCAGGGATCCAAGTAGTCCACAAACCACAACTTCTTTAGACACTTTTGCAACAACTTCTGGCAACTCAAGGGGTCCACGAGCATCTTCACGTACTCAAAGATCTCAAATAAGTATGGATCCTAATTTCAAACCAAGAAGAAGTCCAAGAATTGCATCAAGAGCTACTGTGGACCAAGGAGGACATGAAGGTGCTTTTGCTCGGTTTGGAGAACCATCTGGTCCACACACATCAGTATCTCTGCAAAGAAGACCTGCACAAAAAGGATTGAAGCCTCTTCCTGCTAGTATGCAATGGAAAGAAGACATATACGAGCTAGCATGGGAGGGTACGATCTTCGCCAAGAATCAAGAACGGCTTGTTCTAGTCAGTAAGGCAAAGGCGCACAAGATGAAGTCTGCACCAGAGAGCCTTACTACTGGTTGGCCACGACTGCTGAAGATGGGTAATGTTATACCAGAAGCCACTTTTGGCAGGTCATATGAAAGACAAGCCCATGATTTGATCTTTGAGATTACGGGTGACGAGAAAATATATGACCTACTAAATAAGAACGAGCTGTGTATAAGGTTCATCCTGCCTACTCAGTCACTGATAATTACCCCTGCTGAACACAACCACTTCACTGGGAGAGTGTATGCAGGGGAAGGACCTCTAGTGGAGCAACATCTGGTTGAACAATACAATCAACAAAGGGATCAGCAGCACCAATATGCCACAACTGGTACAAGACTGCGCAATGAGCAAGTCATTGTACTGGATGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM-like Rhamnogal_lyase fn3_3

Protein Analysis

619

Amino Acids

67.58

Weight (kDa)

5.3

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 4 - 154 2.8e-22 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1829
Acc36I ACCTGC 2 cut(s) 1354, 1557
AccI GTMKAC 2 cut(s) 33, 156
AccII CGCG 1 cut(s) 214
AccIII TCCGGA 1 cut(s) 703
AciI CCGC 4 cut(s) 5, 16, 303, 462
AclWI GGATC 8 cut(s) 572, 982, 1094, 1096, 1107, 1205, 1218, 1797
AcoI YGGCCR 3 cut(s) 112, 490, 1517
AcuI CTGAAG 5 cut(s) 123, 583, 603, 999, 1550
AfiI CCNNNNNNNGG 3 cut(s) 166, 312, 414
AgsI TTSAA 6 cut(s) 254, 395, 737, 1222, 1363, 1769
AjnI CCWGG 3 cut(s) 129, 159, 165
AjuI GAANNNNNNNTTGG 2 cut(s) 1545, 1577
AluBI AGCT 8 cut(s) 47, 125, 143, 701, 733, 1256, 1408, 1651
AluI AGCT 8 cut(s) 47, 125, 143, 701, 733, 1256, 1408, 1651
Alw26I GTCTC 3 cut(s) 756, 803, 920
AlwI GGATC 8 cut(s) 572, 982, 1094, 1096, 1107, 1205, 1218, 1797
AlwNI CAGNNNCTG 2 cut(s) 728, 1684
Ama87I CYCGRG 1 cut(s) 806
Aor13HI TCCGGA 1 cut(s) 703
AoxI GGCC 5 cut(s) 53, 112, 163, 490, 1517
ApeKI GCWGC 5 cut(s) 149, 434, 662, 872, 1795
Asp700I GAANNNNTTC 3 cut(s) 592, 644, 864
AspLEI GCGC 3 cut(s) 1039, 1477, 1830
AspS9I GGNCC 7 cut(s) 53, 163, 290, 1166, 1264, 1313, 1742
AsuHPI GGTGA 3 cut(s) 795, 897, 1625
AsuII TTCGAA 1 cut(s) 457
AsuNHI GCTAGC 1 cut(s) 1408
AvaI CYCGRG 1 cut(s) 806
AvaII GGWCC 5 cut(s) 290, 1166, 1264, 1313, 1742
BaeI ACNNNNGTAYC 2 cut(s) 1311, 1344
BalI TGGCCA 3 cut(s) 114, 492, 1519
BamHI GGATCC 2 cut(s) 1099, 1210
BanII GRGCYC 1 cut(s) 261
BarI GAAGNNNNNNTAC 2 cut(s) 675, 707
BauI CACGAG 1 cut(s) 1170
BbsI GAAGAC 2 cut(s) 1348, 1401
BbvCI CCTCAGC 1 cut(s) 561
BbvI GCAGC 5 cut(s) 161, 421, 649, 884, 1807
BccI CCATC 4 cut(s) 236, 513, 1315, 1528
BceAI ACGGC 2 cut(s) 449, 1463
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BciT130I CCWGG 3 cut(s) 131, 161, 167
BclI TGATCA 1 cut(s) 385
BcoDI GTCTC 3 cut(s) 756, 803, 920
BfaI CTAG 7 cut(s) 231, 999, 1377, 1409, 1457, 1748, 1858
BfuAI ACCTGC 2 cut(s) 1354, 1557
BglII AGATCT 1 cut(s) 1193
BisI GCNGC 8 cut(s) 6, 17, 150, 303, 435, 663, 873, 1796
BlsI GCNGC 8 cut(s) 7, 18, 151, 304, 436, 664, 874, 1797
BmcAI AGTACT 2 cut(s) 994, 1003
Bme1390I CCNGG 3 cut(s) 131, 161, 167
Bme18I GGWCC 5 cut(s) 290, 1166, 1264, 1313, 1742
BmeT110I CYCGRG 1 cut(s) 806
BmgT120I GGNCC 7 cut(s) 53, 163, 290, 1166, 1264, 1313, 1742
BmiI GGNNCC 5 cut(s) 54, 205, 1101, 1167, 1212
BmrFI CCNGG 3 cut(s) 131, 161, 167
BmrI ACTGGG 1 cut(s) 1728
BmsI GCATC 5 cut(s) 124, 523, 595, 1184, 1256
BmtI GCTAGC 1 cut(s) 1412
BmuI ACTGGG 1 cut(s) 1728
BpiI GAAGAC 2 cut(s) 1348, 1401
Bpu10I CCTNAGC 1 cut(s) 561
Bpu14I TTCGAA 1 cut(s) 457
BpuEI CTTGAG 2 cut(s) 522, 1144
BsaAI YACGTR 1 cut(s) 1184
BsaJI CCNNGG 3 cut(s) 160, 224, 1267
BsaWI WCCGGW 1 cut(s) 703
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 312, 414
Bse3DI GCAATG 3 cut(s) 306, 1391, 1837
BseAI TCCGGA 1 cut(s) 703
BseBI CCWGG 3 cut(s) 131, 161, 167
BseDI CCNNGG 3 cut(s) 160, 224, 1267
BseGI GGATG 4 cut(s) 139, 247, 1665, 1858
BseLI CCNNNNNNNGG 3 cut(s) 166, 312, 414
BseMI GCAATG 3 cut(s) 306, 1391, 1837
BseMII CTCAG 4 cut(s) 90, 552, 720, 1691
BseXI GCAGC 5 cut(s) 161, 421, 649, 884, 1807
BseYI CCCAGC 1 cut(s) 311
BsgI GTGCAG 4 cut(s) 168, 852, 1332, 1476
Bsh1236I CGCG 1 cut(s) 214
BshFI GGCC 5 cut(s) 55, 114, 165, 492, 1519
BsiHKCI CYCGRG 1 cut(s) 806
BsiSI CCGG 2 cut(s) 704, 984
BslI CCNNNNNNNGG 3 cut(s) 166, 312, 414
BsmAI GTCTC 3 cut(s) 756, 803, 920
BsnI GGCC 5 cut(s) 55, 114, 165, 492, 1519
BsoBI CYCGRG 1 cut(s) 806
Bsp119I TTCGAA 1 cut(s) 457
Bsp1286I GDGCHC 1 cut(s) 261
Bsp13I TCCGGA 1 cut(s) 703
BspACI CCGC 4 cut(s) 5, 16, 303, 462
BspANI GGCC 5 cut(s) 55, 114, 165, 492, 1519
BspCNI CTCAG 4 cut(s) 89, 553, 721, 1690
BspEI TCCGGA 1 cut(s) 703
BspFNI CGCG 1 cut(s) 214
BspLI GGNNCC 5 cut(s) 54, 205, 1101, 1167, 1212
BspMI ACCTGC 2 cut(s) 1354, 1557
BspOI GCTAGC 1 cut(s) 1412
BspPI GGATC 8 cut(s) 572, 982, 1094, 1096, 1107, 1205, 1218, 1797
BspT104I TTCGAA 1 cut(s) 457
BsrDI GCAATG 3 cut(s) 306, 1391, 1837
BssECI CCNNGG 3 cut(s) 160, 224, 1267
BssNAI GTATAC 1 cut(s) 157
BssSI CACGAG 1 cut(s) 1170
BssT1I CCWWGG 2 cut(s) 224, 1267
Bst1107I GTATAC 1 cut(s) 157
Bst2BI CACGAG 1 cut(s) 1170
Bst2UI CCWGG 3 cut(s) 131, 161, 167
Bst4CI ACNGT 5 cut(s) 120, 696, 770, 887, 1261
Bst6I CTCTTC 1 cut(s) 1374
BstBAI YACGTR 1 cut(s) 1184
BstBI TTCGAA 1 cut(s) 457
BstC8I GCNNGC 5 cut(s) 321, 439, 1011, 1035, 1410
BstDEI CTNAG 5 cut(s) 76, 561, 649, 729, 1677
BstF5I GGATG 4 cut(s) 139, 247, 1665, 1858
BstFNI CGCG 1 cut(s) 214
BstHHI GCGC 3 cut(s) 1039, 1477, 1830
BstMAI GTCTC 3 cut(s) 756, 803, 920
BstMWI GCNNNNNNNGC 6 cut(s) 149, 308, 662, 1253, 1525, 1834
BstNI CCWGG 3 cut(s) 131, 161, 167
BstSCI CCNGG 3 cut(s) 129, 159, 165
BstUI CGCG 1 cut(s) 214
BstV1I GCAGC 5 cut(s) 161, 421, 649, 884, 1807
BstV2I GAAGAC 2 cut(s) 1348, 1401
BstX2I RGATCY 5 cut(s) 974, 1088, 1099, 1193, 1210
BstXI CCANNNNNNTGG 2 cut(s) 225, 1718
BstYI RGATCY 5 cut(s) 974, 1088, 1099, 1193, 1210
BstZ17I GTATAC 1 cut(s) 157
BsuRI GGCC 5 cut(s) 55, 114, 165, 492, 1519
BtgZI GCGATG 1 cut(s) 618
BtsCI GGATG 4 cut(s) 139, 247, 1665, 1858
BtsIMutI CAGTG 5 cut(s) 198, 357, 775, 1682, 1716
BveI ACCTGC 2 cut(s) 1354, 1557
Cac8I GCNNGC 5 cut(s) 321, 439, 1011, 1035, 1410
CaiI CAGNNNCTG 2 cut(s) 728, 1684
CfoI GCGC 3 cut(s) 1039, 1477, 1830
Cfr13I GGNCC 7 cut(s) 53, 163, 290, 1166, 1264, 1313, 1742
CviAII CATG 4 cut(s) 659, 1277, 1413, 1589
DdeI CTNAG 5 cut(s) 76, 561, 649, 729, 1677
EaeI YGGCCR 3 cut(s) 112, 490, 1517
Eam1104I CTCTTC 1 cut(s) 1374
EarI CTCTTC 1 cut(s) 1374
Eco130I CCWWGG 2 cut(s) 224, 1267
Eco24I GRGCYC 1 cut(s) 261
Eco47I GGWCC 5 cut(s) 290, 1166, 1264, 1313, 1742
Eco57I CTGAAG 5 cut(s) 123, 583, 603, 999, 1550
Eco88I CYCGRG 1 cut(s) 806
EcoO109I RGGNCCY 2 cut(s) 53, 1742
EcoRII CCWGG 3 cut(s) 129, 159, 165
EcoT14I CCWWGG 2 cut(s) 224, 1267
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 2 cut(s) 224, 1267
FaeI CATG 4 cut(s) 662, 1280, 1416, 1592
FalI AAGNNNNNCTT 6 cut(s) 632, 664, 849, 881, 1434, 1466
FatI CATG 4 cut(s) 658, 1276, 1412, 1588
FauNDI CATATG 1 cut(s) 1573
FbaI TGATCA 1 cut(s) 385
FblI GTMKAC 2 cut(s) 33, 156
Fnu4HI GCNGC 8 cut(s) 6, 17, 150, 303, 435, 663, 873, 1796
FokI GGATG 3 cut(s) 146, 254, 1652
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 8 cut(s) 6, 17, 150, 303, 435, 663, 873, 1796
FspBI CTAG 7 cut(s) 231, 999, 1377, 1409, 1457, 1748, 1858
FspI TGCGCA 1 cut(s) 1829
GlaI GCGC 3 cut(s) 1038, 1476, 1829
GluI GCNGC 8 cut(s) 6, 17, 150, 303, 435, 663, 873, 1796
GsaI CCCAGC 1 cut(s) 315
HaeIII GGCC 5 cut(s) 55, 114, 165, 492, 1519
HapII CCGG 2 cut(s) 704, 984
HhaI GCGC 3 cut(s) 1039, 1477, 1830
Hin1II CATG 4 cut(s) 662, 1280, 1416, 1592
Hin6I GCGC 3 cut(s) 1037, 1475, 1828
HinP1I GCGC 3 cut(s) 1037, 1475, 1828
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HinfI GANTC 4 cut(s) 523, 725, 800, 1438
HpaII CCGG 2 cut(s) 704, 984
HphI GGTGA 3 cut(s) 795, 897, 1625
Hpy166II GTNNAC 9 cut(s) 34, 157, 509, 688, 1075, 1112, 1169, 1264, 1316
Hpy188I TCNGA 7 cut(s) 79, 345, 471, 717, 730, 761, 979
Hpy188III TCNNGA 9 cut(s) 100, 251, 395, 539, 581, 617, 704, 1251, 1442
Hpy8I GTNNAC 9 cut(s) 34, 157, 509, 688, 1075, 1112, 1169, 1264, 1316
Hpy99I CGWCG 2 cut(s) 35, 38
HpyAV CCTTC 8 cut(s) 248, 578, 603, 607, 634, 912, 1274, 1733
HpyCH4III ACNGT 5 cut(s) 120, 696, 770, 887, 1261
HpyCH4IV ACGT 2 cut(s) 621, 1183
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 308, 662, 1253, 1525, 1834
HpyF3I CTNAG 5 cut(s) 76, 561, 649, 729, 1677
HpySE526I ACGT 2 cut(s) 621, 1183
Hsp92II CATG 4 cut(s) 662, 1280, 1416, 1592
HspAI GCGC 3 cut(s) 1037, 1475, 1828
Kpn2I TCCGGA 1 cut(s) 703
Ksp22I TGATCA 1 cut(s) 385
LmnI GCTCC 2 cut(s) 706, 1753
Lsp1109I GCAGC 5 cut(s) 161, 421, 649, 884, 1807
LweI GCATC 5 cut(s) 124, 523, 595, 1184, 1256
MaeI CTAG 7 cut(s) 231, 999, 1377, 1409, 1457, 1748, 1858
MaeII ACGT 2 cut(s) 621, 1183
MaeIII GTNAC 3 cut(s) 194, 1613, 1680
MflI RGATCY 5 cut(s) 974, 1088, 1099, 1193, 1210
MhlI GDGCHC 1 cut(s) 261
MlsI TGGCCA 3 cut(s) 114, 492, 1519
MluCI AATT 6 cut(s) 178, 494, 551, 1216, 1242, 1689
MluNI TGGCCA 3 cut(s) 114, 492, 1519
MlyI GAGTC 2 cut(s) 517, 719
MmeI TCCRAC 1 cut(s) 777
MnlI CCTC 7 cut(s) 95, 556, 646, 1265, 1377, 1411, 1755
Mox20I TGGCCA 3 cut(s) 114, 492, 1519
MroI TCCGGA 1 cut(s) 703
MroXI GAANNNNTTC 3 cut(s) 592, 644, 864
MscI TGGCCA 3 cut(s) 114, 492, 1519
MseI TTAA 1 cut(s) 209
MslI CAYNNNNRTG 2 cut(s) 1281, 1803
Msp20I TGGCCA 3 cut(s) 114, 492, 1519
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 2 cut(s) 704, 984
MspR9I CCNGG 3 cut(s) 131, 161, 167
MvaI CCWGG 3 cut(s) 131, 161, 167
MvnI CGCG 1 cut(s) 214
MwoI GCNNNNNNNGC 6 cut(s) 149, 308, 662, 1253, 1525, 1834
NdeI CATATG 1 cut(s) 1573
NheI GCTAGC 1 cut(s) 1408
NlaIII CATG 4 cut(s) 662, 1280, 1416, 1592
NlaIV GGNNCC 5 cut(s) 54, 205, 1101, 1167, 1212
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 2 cut(s) 1613, 1680
NsbI TGCGCA 1 cut(s) 1829
NspV TTCGAA 1 cut(s) 457
PdmI GAANNNNTTC 3 cut(s) 592, 644, 864
PfeI GAWTC 2 cut(s) 800, 1438
PflFI GACNNNGTC 2 cut(s) 219, 931
PkrI GCNGC 8 cut(s) 7, 18, 151, 304, 436, 664, 874, 1797
PleI GAGTC 2 cut(s) 517, 719
PpsI GAGTC 2 cut(s) 517, 719
Ppu21I YACGTR 1 cut(s) 1184
PpuMI RGGWCCY 1 cut(s) 1742
Psp5II RGGWCCY 1 cut(s) 1742
Psp6I CCWGG 3 cut(s) 129, 159, 165
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 3 cut(s) 129, 159, 165
PspN4I GGNNCC 5 cut(s) 54, 205, 1101, 1167, 1212
PspPI GGNCC 7 cut(s) 53, 163, 290, 1166, 1264, 1313, 1742
PspPPI RGGWCCY 1 cut(s) 1742
PsrI GAACNNNNNNTAC 2 cut(s) 187, 219
PstNI CAGNNNCTG 2 cut(s) 728, 1684
PsuI RGATCY 5 cut(s) 974, 1088, 1099, 1193, 1210
PsyI GACNNNGTC 2 cut(s) 219, 931
PvuII CAGCTG 1 cut(s) 143
RseI CAYNNNNRTG 2 cut(s) 1281, 1803
SalI GTCGAC 1 cut(s) 32
SaqAI TTAA 1 cut(s) 209
SatI GCNGC 8 cut(s) 6, 17, 150, 303, 435, 663, 873, 1796
Sau96I GGNCC 7 cut(s) 53, 163, 290, 1166, 1264, 1313, 1742
ScaI AGTACT 2 cut(s) 994, 1003
SchI GAGTC 2 cut(s) 517, 719
ScrFI CCNGG 3 cut(s) 131, 161, 167
SduI GDGCHC 1 cut(s) 261
SfaNI GCATC 5 cut(s) 124, 523, 595, 1184, 1256
SfuI TTCGAA 1 cut(s) 457
SgrDI CGTCGACG 1 cut(s) 32
SinI GGWCC 5 cut(s) 290, 1166, 1264, 1313, 1742
SmiMI CAYNNNNRTG 2 cut(s) 1281, 1803
SmlI CTYRAG 2 cut(s) 537, 1159
SmoI CTYRAG 2 cut(s) 537, 1159
Sse9I AATT 6 cut(s) 178, 494, 551, 1216, 1242, 1689
SsiI CCGC 4 cut(s) 5, 16, 303, 462
SspMI CTAG 7 cut(s) 231, 999, 1377, 1409, 1457, 1748, 1858
StyD4I CCNGG 3 cut(s) 129, 159, 165
StyI CCWWGG 2 cut(s) 224, 1267
TaaI ACNGT 5 cut(s) 120, 696, 770, 887, 1261
TaiI ACGT 2 cut(s) 624, 1186
TaqI TCGA 5 cut(s) 33, 87, 457, 517, 638
TasI AATT 6 cut(s) 178, 494, 551, 1216, 1242, 1689
TatI WGTACW 5 cut(s) 961, 992, 1001, 1072, 1846
TauI GCSGC 3 cut(s) 8, 19, 305
TfiI GAWTC 2 cut(s) 800, 1438
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TscAI CASTG 5 cut(s) 205, 364, 775, 1689, 1723
TseFI GTSAC 2 cut(s) 1613, 1680
TseI GCWGC 5 cut(s) 149, 434, 662, 872, 1795
Tsp45I GTSAC 2 cut(s) 1613, 1680
TspDTI ATGAA 7 cut(s) 312, 864, 907, 1293, 1499, 1590, 1654
TspGWI ACGGA 2 cut(s) 51, 201
TspRI CASTG 5 cut(s) 205, 364, 775, 1689, 1723
Tth111I GACNNNGTC 2 cut(s) 219, 931
VpaK11BI GGWCC 5 cut(s) 290, 1166, 1264, 1313, 1742
XcmI CCANNNNNNNNNTGG 1 cut(s) 500
XmiI GTMKAC 2 cut(s) 33, 156
XmnI GAANNNNTTC 3 cut(s) 592, 644, 864
XspI CTAG 7 cut(s) 231, 999, 1377, 1409, 1457, 1748, 1858
ZrmI AGTACT 2 cut(s) 994, 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.