Rorug07G0206400

Guanylate-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0005639
Physical Location & Seq
Forward (+)
18190100 .. 18190444
345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0206400.1

Sequence Viewer

Length: 345 bp
ATGACACTCATGAATCATGATGGTAAGAGAAAATGTGAGACCTTATGGCCTATTTTCAAAATTGCACATCAAAAGAGCCGCTACATTTTTGACCTTTACCATCGCAGAAAAGAAACATCCAAGGAATTGTATGAGTTCTGCTTGGACCAAGGTTACGCTGACCGCAATCTAATTGCAAAATGGAAGAAGCTTGGTTATGAACGCCTCTGCTGTTTAAGGTGCATGCAGCCTCGGGATCATAACTTTGCCATCACATGCATTTGCCGAGTGCCCAAGCATCTAAGAGAGGAGAAGGTCATAGAATGCGTCCATTGTGGCTGCAAGGGCTGTGCCAGTGGAGATTGA

Protein Analysis

114

Amino Acids

13.57

Weight (kDa)

9.0

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BUD31 PF01125 5 - 112 1.1e-50 Pre-mRNA-splicing factor BUD31
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 79, 163
AclWI GGATC 1 cut(s) 243
AgsI TTSAA 1 cut(s) 58
AluBI AGCT 1 cut(s) 190
AluI AGCT 1 cut(s) 190
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 1 cut(s) 243
Ama87I CYCGRG 1 cut(s) 231
AoxI GGCC 1 cut(s) 47
ApeKI GCWGC 2 cut(s) 226, 318
AspS9I GGNCC 1 cut(s) 145
AvaI CYCGRG 1 cut(s) 231
AvaII GGWCC 1 cut(s) 145
BaeGI GKGCMC 1 cut(s) 273
BbvI GCAGC 2 cut(s) 238, 305
BccI CCATC 3 cut(s) 14, 108, 257
BcoDI GTCTC 1 cut(s) 32
BisI GCNGC 3 cut(s) 79, 227, 319
BlsI GCNGC 3 cut(s) 80, 228, 320
Bme18I GGWCC 1 cut(s) 145
BmeT110I CYCGRG 1 cut(s) 231
BmgT120I GGNCC 1 cut(s) 145
BmsI GCATC 1 cut(s) 286
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 3 cut(s) 120, 148, 230
Bse1I ACTGG 1 cut(s) 333
BseDI CCNNGG 3 cut(s) 120, 148, 230
BseGI GGATG 1 cut(s) 116
BseNI ACTGG 1 cut(s) 333
BseRI GAGGAG 1 cut(s) 302
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 2 cut(s) 238, 305
BshFI GGCC 1 cut(s) 49
BsiHKCI CYCGRG 1 cut(s) 231
BsmAI GTCTC 1 cut(s) 32
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 1 cut(s) 49
Bso31I GGTCTC 1 cut(s) 32
BsoBI CYCGRG 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 1 cut(s) 235
BspACI CCGC 2 cut(s) 79, 163
BspANI GGCC 1 cut(s) 49
BspHI TCATGA 2 cut(s) 9, 16
BspPI GGATC 1 cut(s) 243
BspTNI GGTCTC 1 cut(s) 32
BsrI ACTGG 1 cut(s) 333
BssECI CCNNGG 3 cut(s) 120, 148, 230
BssMI GATC 1 cut(s) 235
BssT1I CCWWGG 2 cut(s) 120, 148
BstC8I GCNNGC 1 cut(s) 224
BstDEI CTNAG 1 cut(s) 281
BstF5I GGATG 1 cut(s) 116
BstKTI GATC 1 cut(s) 238
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 1 cut(s) 235
BstMWI GCNNNNNNNGC 1 cut(s) 324
BstNSI RCATGY 2 cut(s) 226, 258
BstSLI GKGCMC 1 cut(s) 273
BstV1I GCAGC 2 cut(s) 238, 305
BsuRI GGCC 1 cut(s) 49
BtgZI GCGATG 1 cut(s) 86
BtsCI GGATG 1 cut(s) 116
BtsIMutI CAGTG 1 cut(s) 340
Cac8I GCNNGC 1 cut(s) 224
CciI TCATGA 2 cut(s) 9, 16
Cfr13I GGNCC 1 cut(s) 145
CseI GACGC 1 cut(s) 295
CviAII CATG 4 cut(s) 10, 17, 223, 255
CviJI RGCY 6 cut(s) 49, 78, 190, 229, 318, 327
CviKI_1 RGCY 6 cut(s) 49, 78, 190, 229, 318, 327
DdeI CTNAG 1 cut(s) 281
DpnI GATC 1 cut(s) 237
DpnII GATC 1 cut(s) 235
Eco130I CCWWGG 2 cut(s) 120, 148
Eco31I GGTCTC 1 cut(s) 32
Eco47I GGWCC 1 cut(s) 145
Eco88I CYCGRG 1 cut(s) 231
EcoT14I CCWWGG 2 cut(s) 120, 148
EcoT22I ATGCAT 1 cut(s) 260
ErhI CCWWGG 2 cut(s) 120, 148
FaeI CATG 4 cut(s) 13, 20, 226, 258
FaiI YATR 9 cut(s) 11, 18, 46, 132, 198, 224, 240, 256, 299
FatI CATG 4 cut(s) 9, 16, 222, 254
Fnu4HI GCNGC 3 cut(s) 79, 227, 319
FokI GGATG 1 cut(s) 103
Fsp4HI GCNGC 3 cut(s) 79, 227, 319
GluI GCNGC 3 cut(s) 79, 227, 319
HaeIII GGCC 1 cut(s) 49
HgaI GACGC 1 cut(s) 295
Hin1II CATG 4 cut(s) 13, 20, 226, 258
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 1 cut(s) 13
Hpy188III TCNNGA 3 cut(s) 10, 17, 233
HpyAV CCTTC 1 cut(s) 286
HpyCH4V TGCA 6 cut(s) 65, 176, 222, 226, 258, 321
HpyF10VI GCNNNNNNNGC 1 cut(s) 324
HpyF3I CTNAG 1 cut(s) 281
Hsp92II CATG 4 cut(s) 13, 20, 226, 258
Kzo9I GATC 1 cut(s) 235
Lsp1109I GCAGC 2 cut(s) 238, 305
LweI GCATC 1 cut(s) 286
MaeIII GTNAC 1 cut(s) 152
MalI GATC 1 cut(s) 237
MboI GATC 1 cut(s) 235
MboII GAAGA 1 cut(s) 196
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 3 cut(s) 60, 125, 171
MnlI CCTC 3 cut(s) 215, 240, 280
Mph1103I ATGCAT 1 cut(s) 260
MseI TTAA 1 cut(s) 215
Mva1269I GAATGC 1 cut(s) 308
MwoI GCNNNNNNNGC 1 cut(s) 324
NdeII GATC 1 cut(s) 235
NlaIII CATG 4 cut(s) 13, 20, 226, 258
NmeAIII GCCGAG 1 cut(s) 290
NsiI ATGCAT 1 cut(s) 260
NspI RCATGY 2 cut(s) 226, 258
PaeI GCATGC 1 cut(s) 226
PagI TCATGA 2 cut(s) 9, 16
PctI GAATGC 1 cut(s) 308
PfeI GAWTC 1 cut(s) 13
PkrI GCNGC 3 cut(s) 80, 228, 320
PspPI GGNCC 1 cut(s) 145
SaqAI TTAA 1 cut(s) 215
SatI GCNGC 3 cut(s) 79, 227, 319
Sau3AI GATC 1 cut(s) 235
Sau96I GGNCC 1 cut(s) 145
SduI GDGCHC 1 cut(s) 273
SetI ASST 6 cut(s) 44, 96, 154, 192, 221, 297
SfaNI GCATC 1 cut(s) 286
SinI GGWCC 1 cut(s) 145
SphI GCATGC 1 cut(s) 226
Sse9I AATT 3 cut(s) 60, 125, 171
SsiI CCGC 2 cut(s) 79, 163
StyI CCWWGG 2 cut(s) 120, 148
TasI AATT 3 cut(s) 60, 125, 171
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 1 cut(s) 215
Tru9I TTAA 1 cut(s) 215
TscAI CASTG 1 cut(s) 340
TseI GCWGC 2 cut(s) 226, 318
TspDTI ATGAA 2 cut(s) 26, 213
TspRI CASTG 1 cut(s) 340
VpaK11BI GGWCC 1 cut(s) 145
XceI RCATGY 2 cut(s) 226, 258
Zsp2I ATGCAT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.