Rorug06G0205600

Ribonuclease 3-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003615
Physical Location & Seq
Reverse (-)
32787625 .. 32806052
18428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0205600.1

Sequence Viewer

Length: 1086 bp
ATGTTGAAGGCCAAGAGTTTGAGCAAAGTAGCGGTGCCGCCTTCACTCATACACAATCCTTCTCCCGCTAACATCCAATCCACTCGCCTCGCTCTCCATGTTAGCGAGGATGGCTCTTCTTGTTGGCTCTACATCGCCTCCGGCTGCCAAATCTACAAGCTACAGGTTGCGTTGGATGATTCTCTGGTCAGTGAAGGGAAAGATAGCCTTCTGATCCCTGTACACAGGCAGGTTATGAACTCATTTATGGTTAATCGGTGCCCACATCGTTCAGAAATTCAGAGTATGGTGCTTGCTGAAACTGAGAGCACTGGCAATTCAATGTTGGGAACTGTGGATTCTTATGGTCACCTTATTGTATCTAAACTGGACACTAATGGTGAAGATGTAGAGAGGCTTACTTATTCAGTATTACCTCGTGATTGCGGCATTGGAGAGAGTGGGTGGGCAGGTCTTTGCTTCAATCCAATACAATTGTCCACGGCAGCTGTTGCACGTAGCTTCTGCAAAACCATTGATGTTTATGACCAGGATATCCACGTTAGGACATTATGTACACTCTTGTATCCAACATCACTGAGCTTCATGCAAAGTTCGCGTTATGGAAATGAAAGTTCTATATTAGCTGTCACTGAAGGTTGCCAGCTGAGTATATGGGACTTGAGAATGAAAGAAAATGGCGGCTGTCTACAACGAATATGTGGTTCCCTTGGTGATGTCTTCTACACTGTATGCAGTTCCTCAACTGGAAATATTGCAGTCGGTGGAGCTGATCGTACAGTGACAATCTATGATCCTCGCAGATGGGCATCACTATCAAGATGGGTGAACTGCTCGAAATATGAGATAACTGGACTTGCTTTCTCTTCAGTTGACTCCAACCATATCTATATACAAGGGGTTGATTACGAGGTCTTCTGTGGAGACTGGAAAAGAAACAGCAAGTTATTTTCCTTTAGAGGAGATTCAAATTGGCTTGGTTTTAGCAAGTGCCAGAACAGTGATGTTGTCGGTGGATGGTGTGACTCGGGTAGCATCTTTGTGGCTAATGTTGTAGCTGAAGAGGGTGAAACATTGTTTCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DND1_DSRM

Protein Analysis

361

Amino Acids

39.64

Weight (kDa)

5.54

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 200 - 302 8.9e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 220, 440
AccB1I GGYRCC 2 cut(s) 34, 258
AccI GTMKAC 1 cut(s) 688
AccII CGCG 1 cut(s) 598
AciI CCGC 5 cut(s) 32, 38, 66, 426, 681
AclWI GGATC 2 cut(s) 208, 788
AcsI RAATTY 1 cut(s) 276
AcuI CTGAAG 3 cut(s) 654, 852, 1080
AfaI GTAC 3 cut(s) 222, 556, 778
AgsI TTSAA 4 cut(s) 7, 321, 463, 969
AjnI CCWGG 1 cut(s) 528
AluBI AGCT 8 cut(s) 160, 488, 501, 582, 626, 646, 770, 1058
AluI AGCT 8 cut(s) 160, 488, 501, 582, 626, 646, 770, 1058
Alw21I GWGCWC 1 cut(s) 311
Alw26I GTCTC 1 cut(s) 918
AlwI GGATC 2 cut(s) 208, 788
Ama87I CYCGRG 1 cut(s) 1027
AoxI GGCC 1 cut(s) 9
ApeKI GCWGC 2 cut(s) 144, 485
ApoI RAATTY 1 cut(s) 276
AsuHPI GGTGA 5 cut(s) 341, 392, 725, 838, 1079
AvaI CYCGRG 1 cut(s) 1027
BaeGI GKGCMC 1 cut(s) 263
BaeI ACNNNNGTAYC 2 cut(s) 548, 581
BanI GGYRCC 2 cut(s) 34, 258
BauI CACGAG 1 cut(s) 417
BbsI GAAGAC 2 cut(s) 712, 907
Bbv12I GWGCWC 1 cut(s) 311
BbvI GCAGC 2 cut(s) 131, 497
BccI CCATC 4 cut(s) 104, 798, 816, 1011
BceAI ACGGC 1 cut(s) 498
BciT130I CCWGG 1 cut(s) 530
BciVI GTATCC 1 cut(s) 576
BcoDI GTCTC 1 cut(s) 918
BfmI CTRYAG 1 cut(s) 161
BfuAI ACCTGC 2 cut(s) 220, 440
BfuI GTATCC 1 cut(s) 576
BisI GCNGC 5 cut(s) 38, 145, 427, 486, 682
BlsI GCNGC 5 cut(s) 39, 146, 428, 487, 683
Bme1390I CCNGG 1 cut(s) 530
BmeT110I CYCGRG 1 cut(s) 1027
BmiI GGNNCC 3 cut(s) 36, 260, 706
BmrFI CCNGG 1 cut(s) 530
BmsI GCATC 2 cut(s) 818, 1044
BpiI GAAGAC 2 cut(s) 712, 907
BplI GAGNNNNNCTC 2 cut(s) 98, 130
BpuEI CTTGAG 1 cut(s) 682
BsaAI YACGTR 1 cut(s) 497
BsaBI GATNNNNATC 1 cut(s) 808
BsaJI CCNNGG 2 cut(s) 480, 709
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bse1I ACTGG 5 cut(s) 316, 372, 751, 856, 932
Bse8I GATNNNNATC 1 cut(s) 808
BseBI CCWGG 1 cut(s) 530
BseDI CCNNGG 2 cut(s) 480, 709
BseGI GGATG 4 cut(s) 72, 115, 181, 1022
BseJI GATNNNNATC 1 cut(s) 808
BseMII CTCAG 3 cut(s) 294, 569, 638
BseNI ACTGG 5 cut(s) 316, 372, 751, 856, 932
BseRI GAGGAG 1 cut(s) 975
BseSI GKGCMC 1 cut(s) 263
BseXI GCAGC 2 cut(s) 131, 497
Bsh1236I CGCG 1 cut(s) 598
BshFI GGCC 1 cut(s) 11
BshNI GGYRCC 2 cut(s) 34, 258
BsiHKAI GWGCWC 1 cut(s) 311
BsiHKCI CYCGRG 1 cut(s) 1027
BsiSI CCGG 1 cut(s) 141
BslFI GGGAC 1 cut(s) 671
BsmAI GTCTC 1 cut(s) 918
BsmFI GGGAC 1 cut(s) 671
BsnI GGCC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 1027
Bsp1286I GDGCHC 2 cut(s) 263, 311
Bsp1407I TGTACA 2 cut(s) 220, 554
Bsp143I GATC 3 cut(s) 213, 772, 793
BspACI CCGC 5 cut(s) 32, 38, 66, 426, 681
BspANI GGCC 1 cut(s) 11
BspCNI CTCAG 3 cut(s) 295, 570, 639
BspFNI CGCG 1 cut(s) 598
BspLI GGNNCC 3 cut(s) 36, 260, 706
BspMI ACCTGC 2 cut(s) 220, 440
BspPI GGATC 2 cut(s) 208, 788
BspQI GCTCTTC 1 cut(s) 121
BspT107I GGYRCC 2 cut(s) 34, 258
BsrGI TGTACA 2 cut(s) 220, 554
BsrI ACTGG 5 cut(s) 316, 372, 751, 856, 932
BssECI CCNNGG 2 cut(s) 480, 709
BssMI GATC 3 cut(s) 213, 772, 793
BssSI CACGAG 1 cut(s) 417
BssT1I CCWWGG 1 cut(s) 709
Bst2BI CACGAG 1 cut(s) 417
Bst2UI CCWGG 1 cut(s) 530
Bst4CI ACNGT 4 cut(s) 334, 730, 781, 1001
Bst6I CTCTTC 3 cut(s) 121, 871, 1056
BstAPI GCANNNNNTGC 1 cut(s) 491
BstAUI TGTACA 2 cut(s) 220, 554
BstBAI YACGTR 1 cut(s) 497
BstC8I GCNNGC 2 cut(s) 294, 644
BstDEI CTNAG 3 cut(s) 303, 578, 647
BstDSI CCRYGG 1 cut(s) 480
BstEII GGTNACC 1 cut(s) 347
BstF5I GGATG 4 cut(s) 72, 115, 181, 1022
BstFNI CGCG 1 cut(s) 598
BstKTI GATC 3 cut(s) 216, 775, 796
BstMAI GTCTC 1 cut(s) 918
BstMBI GATC 3 cut(s) 213, 772, 793
BstMWI GCNNNNNNNGC 3 cut(s) 111, 491, 595
BstNI CCWGG 1 cut(s) 530
BstPI GGTNACC 1 cut(s) 347
BstSCI CCNGG 1 cut(s) 528
BstSFI CTRYAG 1 cut(s) 161
BstSLI GKGCMC 1 cut(s) 263
BstUI CGCG 1 cut(s) 598
BstV1I GCAGC 2 cut(s) 131, 497
BstV2I GAAGAC 2 cut(s) 712, 907
BsuI GTATCC 1 cut(s) 576
BsuRI GGCC 1 cut(s) 11
BtgI CCRYGG 1 cut(s) 480
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 4 cut(s) 72, 115, 181, 1022
BtsIMutI CAGTG 7 cut(s) 196, 309, 575, 630, 726, 786, 1006
BveI ACCTGC 2 cut(s) 220, 440
Cac8I GCNNGC 2 cut(s) 294, 644
Csp6I GTAC 3 cut(s) 221, 555, 777
CviAII CATG 2 cut(s) 98, 586
CviQI GTAC 3 cut(s) 221, 555, 777
DdeI CTNAG 3 cut(s) 303, 578, 647
DpnI GATC 3 cut(s) 215, 774, 795
DpnII GATC 3 cut(s) 213, 772, 793
Eam1104I CTCTTC 3 cut(s) 121, 871, 1056
EarI CTCTTC 3 cut(s) 121, 871, 1056
Eco130I CCWWGG 1 cut(s) 709
Eco32I GATATC 1 cut(s) 535
Eco57I CTGAAG 3 cut(s) 654, 852, 1080
Eco88I CYCGRG 1 cut(s) 1027
Eco91I GGTNACC 1 cut(s) 347
EcoO65I GGTNACC 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 528
EcoRV GATATC 1 cut(s) 535
EcoT14I CCWWGG 1 cut(s) 709
ErhI CCWWGG 1 cut(s) 709
FaeI CATG 2 cut(s) 101, 589
FalI AAGNNNNNCTT 2 cut(s) 192, 224
FaqI GGGAC 1 cut(s) 671
FatI CATG 2 cut(s) 97, 585
FauI CCCGC 1 cut(s) 73
FblI GTMKAC 1 cut(s) 688
Fnu4HI GCNGC 5 cut(s) 38, 145, 427, 486, 682
FokI GGATG 4 cut(s) 59, 122, 188, 1029
Fsp4HI GCNGC 5 cut(s) 38, 145, 427, 486, 682
GluI GCNGC 5 cut(s) 38, 145, 427, 486, 682
HaeIII GGCC 1 cut(s) 11
HapII CCGG 1 cut(s) 141
Hin1II CATG 2 cut(s) 101, 589
HincII GTYRAC 1 cut(s) 874
HindII GTYRAC 1 cut(s) 874
HinfI GANTC 5 cut(s) 179, 338, 875, 965, 1025
HpaII CCGG 1 cut(s) 141
HphI GGTGA 5 cut(s) 341, 392, 725, 838, 1079
Hpy166II GTNNAC 6 cut(s) 223, 480, 557, 689, 829, 874
Hpy188I TCNGA 3 cut(s) 213, 274, 282
Hpy188III TCNNGA 2 cut(s) 419, 819
Hpy8I GTNNAC 6 cut(s) 223, 480, 557, 689, 829, 874
HpyAV CCTTC 5 cut(s) 51, 69, 188, 218, 629
HpyCH4III ACNGT 4 cut(s) 334, 730, 781, 1001
HpyCH4IV ACGT 2 cut(s) 496, 540
HpyCH4V TGCA 5 cut(s) 494, 507, 589, 735, 758
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 491, 595
HpyF3I CTNAG 3 cut(s) 303, 578, 647
HpySE526I ACGT 2 cut(s) 496, 540
Hsp92II CATG 2 cut(s) 101, 589
Kzo9I GATC 3 cut(s) 213, 772, 793
LguI GCTCTTC 1 cut(s) 121
LmnI GCTCC 1 cut(s) 767
Lsp1109I GCAGC 2 cut(s) 131, 497
LweI GCATC 2 cut(s) 818, 1044
MaeII ACGT 2 cut(s) 496, 540
MaeIII GTNAC 4 cut(s) 347, 628, 781, 1022
MalI GATC 3 cut(s) 215, 774, 795
MboI GATC 3 cut(s) 213, 772, 793
MboII GAAGA 6 cut(s) 108, 395, 712, 858, 907, 1073
MfeI CAATTG 1 cut(s) 473
MhlI GDGCHC 2 cut(s) 263, 311
MluCI AATT 4 cut(s) 276, 316, 473, 970
MlyI GAGTC 2 cut(s) 869, 1019
MmeI TCCRAC 3 cut(s) 153, 593, 903
MseI TTAA 1 cut(s) 252
MslI CAYNNNNRTG 1 cut(s) 1040
MspA1I CMGCKG 2 cut(s) 488, 646
MspI CCGG 1 cut(s) 141
MspR9I CCNGG 1 cut(s) 530
MunI CAATTG 1 cut(s) 473
MvaI CCWGG 1 cut(s) 530
MvnI CGCG 1 cut(s) 598
MwoI GCNNNNNNNGC 3 cut(s) 111, 491, 595
NdeII GATC 3 cut(s) 213, 772, 793
NlaIII CATG 2 cut(s) 101, 589
NlaIV GGNNCC 3 cut(s) 36, 260, 706
NmuCI GTSAC 4 cut(s) 347, 628, 781, 1022
PciSI GCTCTTC 1 cut(s) 121
PfeI GAWTC 3 cut(s) 179, 338, 965
PkrI GCNGC 5 cut(s) 39, 146, 428, 487, 683
PleI GAGTC 2 cut(s) 869, 1019
PpsI GAGTC 2 cut(s) 869, 1019
Ppu21I YACGTR 1 cut(s) 497
Psp6I CCWGG 1 cut(s) 528
PspEI GGTNACC 1 cut(s) 347
PspGI CCWGG 1 cut(s) 528
PspN4I GGNNCC 3 cut(s) 36, 260, 706
PvuII CAGCTG 2 cut(s) 488, 646
RsaI GTAC 3 cut(s) 222, 556, 778
RsaNI GTAC 3 cut(s) 221, 555, 777
RseI CAYNNNNRTG 1 cut(s) 1040
SapI GCTCTTC 1 cut(s) 121
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 5 cut(s) 38, 145, 427, 486, 682
Sau3AI GATC 3 cut(s) 213, 772, 793
SchI GAGTC 2 cut(s) 869, 1019
ScrFI CCNGG 1 cut(s) 530
SduI GDGCHC 2 cut(s) 263, 311
SfaNI GCATC 2 cut(s) 818, 1044
SfcI CTRYAG 1 cut(s) 161
SmiMI CAYNNNNRTG 1 cut(s) 1040
SmlI CTYRAG 1 cut(s) 661
SmoI CTYRAG 1 cut(s) 661
Sse9I AATT 4 cut(s) 276, 316, 473, 970
SsiI CCGC 5 cut(s) 32, 38, 66, 426, 681
SspI AATATT 1 cut(s) 754
StyD4I CCNGG 1 cut(s) 528
StyI CCWWGG 1 cut(s) 709
TaaI ACNGT 4 cut(s) 334, 730, 781, 1001
TaiI ACGT 2 cut(s) 499, 543
TaqI TCGA 1 cut(s) 836
TasI AATT 4 cut(s) 276, 316, 473, 970
TatI WGTACW 2 cut(s) 220, 554
TauI GCSGC 3 cut(s) 40, 429, 684
TfiI GAWTC 3 cut(s) 179, 338, 965
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 7 cut(s) 196, 316, 582, 637, 733, 786, 1006
TseFI GTSAC 4 cut(s) 347, 628, 781, 1022
TseI GCWGC 2 cut(s) 144, 485
Tsp45I GTSAC 4 cut(s) 347, 628, 781, 1022
TspDTI ATGAA 4 cut(s) 251, 574, 624, 683
TspRI CASTG 7 cut(s) 196, 316, 582, 637, 733, 786, 1006
XapI RAATTY 1 cut(s) 276
XmiI GTMKAC 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.