Rroxscaffold_7G00177760

Ribonuclease 3-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003615
Physical Location & Seq
Forward (+)
17367696 .. 17368222
527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00177760.1

Sequence Viewer

Length: 321 bp
ATGAATGCAGAAAATGCTCTTGATCAACTACAGGCCAAGTTCGCCAAGAGGGAAGTTAAAGTACTGAACAATAGATCAAAACTATATATCGTATGTGATGCATACAAATGGAAGCACCCTGTGTATGAGTGCTGTAAAGAGGATGGGCCGGCCCACCTGAGGATGTTCACCTTCAAGGTCGTTATTGAGATAAAAAAGGAACCCTCAGAGCCAAACACAGAAGTGGAATGTTTTAGTGCACCGCAGCTGAATAAGAAAGCCGCAGCGGAACACGCCGCAGAAGGAGCGTTATGGTACTTGAAGAAGGTTGGTTACGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DND1_DSRM

Protein Analysis

106

Amino Acids

12.18

Weight (kDa)

8.31

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DND1_DSRM PF14709 25 - 101 1.3e-17 double strand RNA binding domain from DEAD END PROTEIN 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 242, 261, 266, 276
AdeI CACNNNGTG 1 cut(s) 121
AfaI GTAC 2 cut(s) 63, 296
AfiI CCNNNNNNNGG 1 cut(s) 159
AgsI TTSAA 2 cut(s) 175, 301
AleI CACNNNNGTG 1 cut(s) 221
AluBI AGCT 1 cut(s) 247
AluI AGCT 1 cut(s) 247
Alw21I GWGCWC 1 cut(s) 241
Alw44I GTGCAC 1 cut(s) 237
AoxI GGCC 3 cut(s) 33, 146, 150
ApaLI GTGCAC 1 cut(s) 237
ApeKI GCWGC 2 cut(s) 244, 263
AspS9I GGNCC 2 cut(s) 146, 151
AsuHPI GGTGA 1 cut(s) 160
AxyI CCTNAGG 1 cut(s) 158
BaeGI GKGCMC 1 cut(s) 241
BarI GAAGNNNNNNTAC 3 cut(s) 45, 77, 296
Bbv12I GWGCWC 1 cut(s) 241
BbvI GCAGC 2 cut(s) 256, 275
BccI CCATC 1 cut(s) 137
BclI TGATCA 1 cut(s) 22
BfmI CTRYAG 1 cut(s) 29
BisI GCNGC 4 cut(s) 245, 261, 264, 276
BlsI GCNGC 4 cut(s) 246, 262, 265, 277
BmcAI AGTACT 1 cut(s) 63
BmgT120I GGNCC 2 cut(s) 146, 151
BmiI GGNNCC 1 cut(s) 201
BmsI GCATC 1 cut(s) 88
Bsc4I CCNNNNNNNGG 1 cut(s) 159
Bse118I RCCGGY 1 cut(s) 148
Bse21I CCTNAGG 1 cut(s) 158
BseGI GGATG 2 cut(s) 148, 168
BseLI CCNNNNNNNGG 1 cut(s) 159
BseMII CTCAG 2 cut(s) 149, 219
BseSI GKGCMC 1 cut(s) 241
BseXI GCAGC 2 cut(s) 256, 275
BshFI GGCC 3 cut(s) 35, 148, 152
BsiHKAI GWGCWC 1 cut(s) 241
BsiSI CCGG 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 159
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 3 cut(s) 35, 148, 152
Bsp1286I GDGCHC 1 cut(s) 241
Bsp143I GATC 2 cut(s) 22, 74
BspACI CCGC 4 cut(s) 242, 261, 266, 276
BspANI GGCC 3 cut(s) 35, 148, 152
BspCNI CTCAG 2 cut(s) 150, 218
BspLI GGNNCC 1 cut(s) 201
BsrFI RCCGGY 1 cut(s) 148
BssAI RCCGGY 1 cut(s) 148
BssMI GATC 2 cut(s) 22, 74
BstAPI GCANNNNNTGC 1 cut(s) 14
BstC8I GCNNGC 1 cut(s) 150
BstDEI CTNAG 2 cut(s) 158, 205
BstF5I GGATG 2 cut(s) 148, 168
BstKTI GATC 2 cut(s) 25, 77
BstMBI GATC 2 cut(s) 22, 74
BstMWI GCNNNNNNNGC 4 cut(s) 14, 41, 272, 284
BstSFI CTRYAG 1 cut(s) 29
BstSLI GKGCMC 1 cut(s) 241
BstV1I GCAGC 2 cut(s) 256, 275
Bsu36I CCTNAGG 1 cut(s) 158
BsuRI GGCC 3 cut(s) 35, 148, 152
BtsCI GGATG 2 cut(s) 148, 168
Cac8I GCNNGC 1 cut(s) 150
Cfr10I RCCGGY 1 cut(s) 148
Cfr13I GGNCC 2 cut(s) 146, 151
Csp6I GTAC 2 cut(s) 62, 295
CviJI RGCY 6 cut(s) 35, 148, 152, 211, 247, 260
CviKI_1 RGCY 6 cut(s) 35, 148, 152, 211, 247, 260
CviQI GTAC 2 cut(s) 62, 295
DdeI CTNAG 2 cut(s) 158, 205
DpnI GATC 2 cut(s) 24, 76
DpnII GATC 2 cut(s) 22, 74
DraIII CACNNNGTG 1 cut(s) 121
Eco81I CCTNAGG 1 cut(s) 158
EcoT22I ATGCAT 1 cut(s) 103
FaiI YATR 6 cut(s) 85, 87, 94, 103, 126, 292
FbaI TGATCA 1 cut(s) 22
Fnu4HI GCNGC 4 cut(s) 245, 261, 264, 276
FokI GGATG 2 cut(s) 155, 175
FseI GGCCGGCC 1 cut(s) 152
Fsp4HI GCNGC 4 cut(s) 245, 261, 264, 276
GluI GCNGC 4 cut(s) 245, 261, 264, 276
HaeIII GGCC 3 cut(s) 35, 148, 152
HapII CCGG 1 cut(s) 149
HpaII CCGG 1 cut(s) 149
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 2 cut(s) 168, 239
Hpy188I TCNGA 1 cut(s) 208
Hpy188III TCNNGA 1 cut(s) 20
Hpy8I GTNNAC 2 cut(s) 168, 239
HpyAV CCTTC 3 cut(s) 181, 275, 298
HpyCH4V TGCA 3 cut(s) 8, 101, 239
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 41, 272, 284
HpyF3I CTNAG 2 cut(s) 158, 205
KroI GCCGGC 1 cut(s) 148
KroNI GCCGGC 1 cut(s) 150
Ksp22I TGATCA 1 cut(s) 22
Kzo9I GATC 2 cut(s) 22, 74
LmnI GCTCC 1 cut(s) 284
LpnPI CCDG 4 cut(s) 17, 132, 162, 170
Lsp1109I GCAGC 2 cut(s) 256, 275
LweI GCATC 1 cut(s) 88
MaeIII GTNAC 1 cut(s) 311
MalI GATC 2 cut(s) 24, 76
MboI GATC 2 cut(s) 22, 74
MboII GAAGA 1 cut(s) 313
MhlI GDGCHC 1 cut(s) 241
MnlI CCTC 4 cut(s) 42, 133, 153, 214
Mph1103I ATGCAT 1 cut(s) 103
MroNI GCCGGC 1 cut(s) 148
MseI TTAA 2 cut(s) 57, 319
MslI CAYNNNNRTG 2 cut(s) 106, 221
MspA1I CMGCKG 2 cut(s) 247, 266
MspI CCGG 1 cut(s) 149
Mva1269I GAATGC 1 cut(s) 10
MwoI GCNNNNNNNGC 4 cut(s) 14, 41, 272, 284
NaeI GCCGGC 1 cut(s) 150
NdeII GATC 2 cut(s) 22, 74
NgoMIV GCCGGC 1 cut(s) 148
NlaIV GGNNCC 1 cut(s) 201
NsiI ATGCAT 1 cut(s) 103
OliI CACNNNNGTG 1 cut(s) 221
PctI GAATGC 1 cut(s) 10
PdiI GCCGGC 1 cut(s) 150
PkrI GCNGC 4 cut(s) 246, 262, 265, 277
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 2 cut(s) 146, 151
PvuII CAGCTG 1 cut(s) 247
RigI GGCCGGCC 1 cut(s) 152
RsaI GTAC 2 cut(s) 63, 296
RsaNI GTAC 2 cut(s) 62, 295
RseI CAYNNNNRTG 2 cut(s) 106, 221
SaqAI TTAA 2 cut(s) 57, 319
SatI GCNGC 4 cut(s) 245, 261, 264, 276
Sau3AI GATC 2 cut(s) 22, 74
Sau96I GGNCC 2 cut(s) 146, 151
ScaI AGTACT 1 cut(s) 63
SduI GDGCHC 1 cut(s) 241
SetI ASST 5 cut(s) 159, 173, 180, 249, 309
SfaNI GCATC 1 cut(s) 88
SfcI CTRYAG 1 cut(s) 29
SmiMI CAYNNNNRTG 2 cut(s) 106, 221
SsiI CCGC 4 cut(s) 242, 261, 266, 276
TatI WGTACW 1 cut(s) 61
TauI GCSGC 2 cut(s) 263, 278
Tru1I TTAA 2 cut(s) 57, 319
Tru9I TTAA 2 cut(s) 57, 319
TseI GCWGC 2 cut(s) 244, 263
TspDTI ATGAA 1 cut(s) 17
VneI GTGCAC 1 cut(s) 237
ZrmI AGTACT 1 cut(s) 63
Zsp2I ATGCAT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.