Rorug07G0226800

Histone H3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008921
Physical Location & Seq
Forward (+)
20696727 .. 20697252
526 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0226800.1

Sequence Viewer

Length: 405 bp
ATGAACACGGCGGCGAGGAATGCCTGGACTTTGTTCCGACGGCCGAGTCAGGGGCTCGCCGAGTCACTGAGTCCGGCGACTCACCACCGCCACGAGCAAGTGAGAGGGATTCGCGTGAAGGTTCTGAACGGAAACTTGGAGTGGGCGCTGACGTTTATGCAGAGGAAGATGCAGTCGAGTGGGATCGAGAGGATGATCAAGCGCGAGCAGACCCACCACATCAAGAACTCCGAGAAGCGCGTGTTGGCACGCAAGAACCTCGAGCGTAAGCTCAAATCCCAAGACCTCGCCCGCAAGCTCCAGTCCATCCTCATCAAGAAAGTCAGATTGTTGCTTGTTTGGGGTTTAGGGTTTAGAATTGTGTTTGTGGAATTGGGACAGGCAATTCAAAGTAGACAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05202 ko05322 map05034 map05202 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

134

Amino Acids

15.66

Weight (kDa)

11.8

Isoelectric Point (pI)

50.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21 PF01165 39 - 91 2.9e-10 Ribosomal protein S21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 45
AccI GTMKAC 1 cut(s) 394
AccII CGCG 3 cut(s) 114, 204, 240
AciI CCGC 3 cut(s) 11, 88, 292
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 1 cut(s) 41
AfiI CCNNNNNNNGG 1 cut(s) 50
AgsI TTSAA 1 cut(s) 389
AjnI CCWGG 1 cut(s) 23
AjuI GAANNNNNNNTTGG 4 cut(s) 119, 151, 227, 259
AluBI AGCT 2 cut(s) 271, 298
AluI AGCT 2 cut(s) 271, 298
AlwI GGATC 1 cut(s) 191
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 1 cut(s) 41
AspLEI GCGC 3 cut(s) 148, 204, 240
AsuHPI GGTGA 1 cut(s) 74
AvaI CYCGRG 1 cut(s) 260
BanII GRGCYC 1 cut(s) 57
BauI CACGAG 1 cut(s) 92
BccI CCATC 1 cut(s) 314
BceAI ACGGC 2 cut(s) 24, 56
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BciT130I CCWGG 1 cut(s) 25
BclI TGATCA 1 cut(s) 195
BfoI RGCGCY 1 cut(s) 149
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 25
BmeT110I CYCGRG 1 cut(s) 260
BmrFI CCNGG 1 cut(s) 25
BmsI GCATC 1 cut(s) 159
BpmI CTGGAG 1 cut(s) 284
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse1I ACTGG 1 cut(s) 301
BseBI CCWGG 1 cut(s) 25
BseGI GGATG 2 cut(s) 198, 306
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMII CTCAG 1 cut(s) 59
BseNI ACTGG 1 cut(s) 301
BseX3I CGGCCG 1 cut(s) 41
Bsh1236I CGCG 3 cut(s) 114, 204, 240
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 1 cut(s) 43
BsiEI CGRYCG 1 cut(s) 44
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 1 cut(s) 390
BslI CCNNNNNNNGG 1 cut(s) 50
BsmFI GGGAC 1 cut(s) 390
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 1 cut(s) 43
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 2 cut(s) 183, 195
BspACI CCGC 3 cut(s) 11, 88, 292
BspANI GGCC 1 cut(s) 43
BspCNI CTCAG 1 cut(s) 60
BspFNI CGCG 3 cut(s) 114, 204, 240
BspPI GGATC 1 cut(s) 191
BsrI ACTGG 1 cut(s) 301
BssMI GATC 2 cut(s) 183, 195
BssSI CACGAG 1 cut(s) 92
Bst2BI CACGAG 1 cut(s) 92
Bst2UI CCWGG 1 cut(s) 25
BstC8I GCNNGC 5 cut(s) 57, 206, 250, 292, 296
BstDEI CTNAG 1 cut(s) 68
BstF5I GGATG 2 cut(s) 198, 306
BstFNI CGCG 3 cut(s) 114, 204, 240
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 3 cut(s) 148, 204, 240
BstKTI GATC 2 cut(s) 186, 198
BstMBI GATC 2 cut(s) 183, 195
BstMCI CGRYCG 1 cut(s) 44
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstNI CCWGG 1 cut(s) 25
BstSCI CCNGG 1 cut(s) 23
BstUI CGCG 3 cut(s) 114, 204, 240
BstZI CGGCCG 1 cut(s) 41
BsuRI GGCC 1 cut(s) 43
BtsCI GGATG 2 cut(s) 198, 306
BtsIMutI CAGTG 1 cut(s) 65
Cac8I GCNNGC 5 cut(s) 57, 206, 250, 292, 296
CfoI GCGC 3 cut(s) 148, 204, 240
CviJI RGCY 4 cut(s) 43, 55, 271, 298
CviKI_1 RGCY 4 cut(s) 43, 55, 271, 298
DdeI CTNAG 1 cut(s) 68
DpnI GATC 2 cut(s) 185, 197
DpnII GATC 2 cut(s) 183, 195
DrdI GACNNNNNNGTC 1 cut(s) 45
DseDI GACNNNNNNGTC 1 cut(s) 45
EaeI YGGCCR 1 cut(s) 41
EagI CGGCCG 1 cut(s) 41
EclXI CGGCCG 1 cut(s) 41
Eco24I GRGCYC 1 cut(s) 57
Eco52I CGGCCG 1 cut(s) 41
Eco88I CYCGRG 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 23
EcoT38I GRGCYC 1 cut(s) 57
FaiI YATR 1 cut(s) 158
FaqI GGGAC 1 cut(s) 390
FauI CCCGC 1 cut(s) 299
FbaI TGATCA 1 cut(s) 195
FblI GTMKAC 1 cut(s) 394
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 2 cut(s) 205, 293
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 1 cut(s) 12
GlaI GCGC 3 cut(s) 147, 203, 239
GluI GCNGC 1 cut(s) 12
GsuI CTGGAG 1 cut(s) 284
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 1 cut(s) 43
HapII CCGG 1 cut(s) 74
HhaI GCGC 3 cut(s) 148, 204, 240
Hin6I GCGC 3 cut(s) 146, 202, 238
HinP1I GCGC 3 cut(s) 146, 202, 238
HinfI GANTC 5 cut(s) 46, 62, 70, 79, 109
HpaII CCGG 1 cut(s) 74
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 1 cut(s) 395
Hpy188I TCNGA 4 cut(s) 38, 126, 232, 326
Hpy188III TCNNGA 3 cut(s) 187, 223, 316
Hpy8I GTNNAC 1 cut(s) 395
Hpy99I CGWCG 1 cut(s) 42
HpyAV CCTTC 1 cut(s) 112
HpyCH4IV ACGT 1 cut(s) 152
HpyCH4V TGCA 2 cut(s) 160, 172
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 1 cut(s) 68
HpySE526I ACGT 1 cut(s) 152
HspAI GCGC 3 cut(s) 146, 202, 238
Ksp22I TGATCA 1 cut(s) 195
Kzo9I GATC 2 cut(s) 183, 195
LmnI GCTCC 1 cut(s) 303
LpnPI CCDG 6 cut(s) 10, 35, 37, 87, 314, 365
LweI GCATC 1 cut(s) 159
MaeII ACGT 1 cut(s) 152
MaeIII GTNAC 1 cut(s) 63
MalI GATC 2 cut(s) 185, 197
MboI GATC 2 cut(s) 183, 195
MboII GAAGA 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 3 cut(s) 357, 371, 384
MlyI GAGTC 4 cut(s) 55, 71, 73, 79
MmeI TCCRAC 1 cut(s) 61
MnlI CCTC 7 cut(s) 9, 98, 156, 183, 269, 296, 320
MspI CCGG 1 cut(s) 74
MspR9I CCNGG 1 cut(s) 25
Mva1269I GAATGC 1 cut(s) 25
MvaI CCWGG 1 cut(s) 25
MvnI CGCG 3 cut(s) 114, 204, 240
MwoI GCNNNNNNNGC 1 cut(s) 20
NdeII GATC 2 cut(s) 183, 195
NmeAIII GCCGAG 2 cut(s) 69, 85
NmuCI GTSAC 1 cut(s) 63
PaeR7I CTCGAG 1 cut(s) 260
PctI GAATGC 1 cut(s) 25
PfeI GAWTC 1 cut(s) 109
PkrI GCNGC 1 cut(s) 13
PleI GAGTC 4 cut(s) 54, 70, 73, 78
PpsI GAGTC 4 cut(s) 54, 70, 73, 78
Psp6I CCWGG 1 cut(s) 23
PspGI CCWGG 1 cut(s) 23
PspXI VCTCGAGB 1 cut(s) 260
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 2 cut(s) 183, 195
SchI GAGTC 4 cut(s) 55, 71, 73, 79
ScrFI CCNGG 1 cut(s) 25
SduI GDGCHC 1 cut(s) 57
SetI ASST 6 cut(s) 123, 155, 261, 273, 288, 300
SfaNI GCATC 1 cut(s) 159
Sfr274I CTCGAG 1 cut(s) 260
SlaI CTCGAG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 3 cut(s) 357, 371, 384
SsiI CCGC 3 cut(s) 11, 88, 292
StyD4I CCNGG 1 cut(s) 23
TaiI ACGT 1 cut(s) 155
TaqI TCGA 3 cut(s) 176, 186, 261
TasI AATT 3 cut(s) 357, 371, 384
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 1 cut(s) 109
TscAI CASTG 1 cut(s) 72
TseFI GTSAC 1 cut(s) 63
Tsp45I GTSAC 1 cut(s) 63
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 1 cut(s) 72
XhoI CTCGAG 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.