Rh5DG521000

Histone H3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008921
Physical Location & Seq
Reverse (-)
80904372 .. 80904785
414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG521000.1

Sequence Viewer

Length: 414 bp
ATGGCTCGCACTAAGCAAACTGCTCGCAAGTCAACTGGAGGGAAAGCTCCAAGGAAGAGTCTGGCCACCAAGATTGCTTATAAGTCTGCCCCAAGCACTGCTGGAGGTGTCAAGAAGCCACACAGATACCGCCCTGGTACTGTTGCTCTTCGCGAAATCCGCAAGTACCAGAAAAGTACTGATCTTCTGATCAGGAAGCTGCCATTCCAGAGGCTTGTTCGCGAAACTGCGCAGAACTACAAGAGTGATCTACGTTTTAATAGCCATGCGGTGATGGCATTGCAGGAGGCTTCTGAGGCTTACCTTGTGGGGCTGTTCGAGGACACGAATCTCTGCGCTATCCATGCAAAGCGAGTTACCATCATGCCGAAAGATATGCAGCTGGCTCGGAGGATCAGGGGTGATCTGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05202 ko05322 map05034 map05202 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

137

Amino Acids

15.49

Weight (kDa)

10.76

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 45 - 132 8.5e-36 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 81
Acc16I TGCGCA 1 cut(s) 231
AccII CGCG 2 cut(s) 153, 222
AciI CCGC 3 cut(s) 130, 160, 269
AclWI GGATC 1 cut(s) 401
AcoI YGGCCR 1 cut(s) 63
AfaI GTAC 3 cut(s) 139, 167, 178
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 3 cut(s) 47, 199, 382
AluI AGCT 3 cut(s) 47, 199, 382
AlwI GGATC 1 cut(s) 401
AoxI GGCC 1 cut(s) 63
ApeKI GCWGC 2 cut(s) 199, 379
AspLEI GCGC 2 cut(s) 232, 338
AsuHPI GGTGA 2 cut(s) 283, 413
BalI TGGCCA 1 cut(s) 65
BbvI GCAGC 2 cut(s) 186, 391
BccI CCATC 2 cut(s) 268, 368
BcgI CGANNNNNNTGC 6 cut(s) 5, 39, 358, 368, 392, 402
BciT130I CCWGG 1 cut(s) 135
BclI TGATCA 1 cut(s) 189
BisI GCNGC 2 cut(s) 200, 380
BlsI GCNGC 2 cut(s) 201, 381
BmcAI AGTACT 1 cut(s) 178
Bme1390I CCNGG 1 cut(s) 135
BmrFI CCNGG 1 cut(s) 135
BpmI CTGGAG 2 cut(s) 57, 123
BsaJI CCNNGG 2 cut(s) 50, 133
Bse1I ACTGG 1 cut(s) 40
Bse3DI GCAATG 1 cut(s) 278
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 2 cut(s) 50, 133
BseMI GCAATG 1 cut(s) 278
BseMII CTCAG 1 cut(s) 285
BseNI ACTGG 1 cut(s) 40
BseXI GCAGC 2 cut(s) 186, 391
Bsh1236I CGCG 2 cut(s) 153, 222
BshFI GGCC 1 cut(s) 65
BsnI GGCC 1 cut(s) 65
Bsp143I GATC 5 cut(s) 181, 189, 247, 393, 403
Bsp68I TCGCGA 2 cut(s) 153, 222
BspACI CCGC 3 cut(s) 130, 160, 269
BspANI GGCC 1 cut(s) 65
BspCNI CTCAG 1 cut(s) 286
BspFNI CGCG 2 cut(s) 153, 222
BspPI GGATC 1 cut(s) 401
BspQI GCTCTTC 1 cut(s) 153
BsrDI GCAATG 1 cut(s) 278
BsrI ACTGG 1 cut(s) 40
BssECI CCNNGG 2 cut(s) 50, 133
BssMI GATC 5 cut(s) 181, 189, 247, 393, 403
BssT1I CCWWGG 1 cut(s) 50
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 1 cut(s) 142
Bst6I CTCTTC 2 cut(s) 50, 153
BstC8I GCNNGC 3 cut(s) 7, 25, 384
BstDEI CTNAG 2 cut(s) 12, 294
BstFNI CGCG 2 cut(s) 153, 222
BstHHI GCGC 2 cut(s) 232, 338
BstKTI GATC 5 cut(s) 184, 192, 250, 396, 406
BstMBI GATC 5 cut(s) 181, 189, 247, 393, 403
BstMWI GCNNNNNNNGC 4 cut(s) 159, 275, 296, 344
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 1 cut(s) 133
BstUI CGCG 2 cut(s) 153, 222
BstV1I GCAGC 2 cut(s) 186, 391
BsuRI GGCC 1 cut(s) 65
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 96
BtuMI TCGCGA 2 cut(s) 153, 222
Cac8I GCNNGC 3 cut(s) 7, 25, 384
CfoI GCGC 2 cut(s) 232, 338
Csp6I GTAC 3 cut(s) 138, 166, 177
CspCI CAANNNNNGTGG 2 cut(s) 55, 90
CviAII CATG 3 cut(s) 266, 344, 364
CviQI GTAC 3 cut(s) 138, 166, 177
DdeI CTNAG 2 cut(s) 12, 294
DpnI GATC 5 cut(s) 183, 191, 249, 395, 405
DpnII GATC 5 cut(s) 181, 189, 247, 393, 403
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 2 cut(s) 50, 153
EarI CTCTTC 2 cut(s) 50, 153
Eco130I CCWWGG 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 3 cut(s) 269, 347, 367
FaiI YATR 5 cut(s) 81, 267, 345, 365, 377
FatI CATG 3 cut(s) 265, 343, 363
FbaI TGATCA 1 cut(s) 189
Fnu4HI GCNGC 2 cut(s) 200, 380
Fsp4HI GCNGC 2 cut(s) 200, 380
FspI TGCGCA 1 cut(s) 231
GlaI GCGC 2 cut(s) 231, 337
GluI GCNGC 2 cut(s) 200, 380
GsuI CTGGAG 2 cut(s) 57, 123
HaeIII GGCC 1 cut(s) 65
HhaI GCGC 2 cut(s) 232, 338
Hin1II CATG 3 cut(s) 269, 347, 367
Hin6I GCGC 2 cut(s) 230, 336
HinP1I GCGC 2 cut(s) 230, 336
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HinfI GANTC 2 cut(s) 58, 328
HphI GGTGA 2 cut(s) 283, 413
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 3 cut(s) 189, 295, 390
Hpy188III TCNNGA 5 cut(s) 112, 152, 193, 208, 221
Hpy8I GTNNAC 1 cut(s) 33
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 4 cut(s) 283, 347, 379, 409
HpyF10VI GCNNNNNNNGC 4 cut(s) 159, 275, 296, 344
HpyF3I CTNAG 2 cut(s) 12, 294
HpySE526I ACGT 1 cut(s) 253
Hsp92II CATG 3 cut(s) 269, 347, 367
HspAI GCGC 2 cut(s) 230, 336
Ksp22I TGATCA 1 cut(s) 189
Kzo9I GATC 5 cut(s) 181, 189, 247, 393, 403
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 1 cut(s) 52
Lsp1109I GCAGC 2 cut(s) 186, 391
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 1 cut(s) 355
MalI GATC 5 cut(s) 183, 191, 249, 395, 405
MboI GATC 5 cut(s) 181, 189, 247, 393, 403
MboII GAAGA 3 cut(s) 67, 140, 176
MlsI TGGCCA 1 cut(s) 65
MluNI TGGCCA 1 cut(s) 65
MlyI GAGTC 1 cut(s) 67
MnlI CCTC 7 cut(s) 32, 98, 204, 280, 289, 313, 384
Mox20I TGGCCA 1 cut(s) 65
MscI TGGCCA 1 cut(s) 65
MseI TTAA 1 cut(s) 258
Msp20I TGGCCA 1 cut(s) 65
MspA1I CMGCKG 1 cut(s) 382
MspR9I CCNGG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 135
MvnI CGCG 2 cut(s) 153, 222
MwoI GCNNNNNNNGC 4 cut(s) 159, 275, 296, 344
NdeII GATC 5 cut(s) 181, 189, 247, 393, 403
NlaIII CATG 3 cut(s) 269, 347, 367
NruI TCGCGA 2 cut(s) 153, 222
NsbI TGCGCA 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 153
PfeI GAWTC 1 cut(s) 328
PkrI GCNGC 2 cut(s) 201, 381
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
PsiI TTATAA 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PvuII CAGCTG 1 cut(s) 382
RruI TCGCGA 2 cut(s) 153, 222
RsaI GTAC 3 cut(s) 139, 167, 178
RsaNI GTAC 3 cut(s) 138, 166, 177
SapI GCTCTTC 1 cut(s) 153
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 2 cut(s) 200, 380
Sau3AI GATC 5 cut(s) 181, 189, 247, 393, 403
ScaI AGTACT 1 cut(s) 178
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 1 cut(s) 135
SetI ASST 6 cut(s) 49, 109, 201, 256, 306, 384
SsiI CCGC 3 cut(s) 130, 160, 269
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 1 cut(s) 142
TaiI ACGT 1 cut(s) 256
TaqI TCGA 1 cut(s) 318
TatI WGTACW 1 cut(s) 176
TfiI GAWTC 1 cut(s) 328
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 1 cut(s) 103
TseI GCWGC 2 cut(s) 199, 379
TspRI CASTG 1 cut(s) 103
ZrmI AGTACT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.