AT4G40030

Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001010
Physical Location & Seq
Reverse (-)
18555532 .. 18557106
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G40030.4

Sequence Viewer

Length: 411 bp
ATGGCTCGTACCAAGCAAACCGCTCGTAAGTCCACCGGAGGTAAAGCTCCAAGGAAGCAACTTGCTACTAAGGCTGCTCGTAAATCTGCACCAACTACTGGTGGAGTCAAGAAACCACATCGTTACCGTCCTGGAACTGTTGCTCTCCGTGAAATCCGTAAGTACCAGAAGAGTACTGAGTTGCTTATCAGGAAACTGCCATTTCAGAGGCTAGTCCGTGAGATTGCTCAAGATTTCAAGACTGATTTGCGTTTCCAGAGCCATGCTGTCTTAGCTCTCCAGGAAGCTGCAGAAGCATATCTTGTTGGTCTCTTTGAAGACACTAACCTTTGTGCCATTCATGCCAAGCGTGTGACCATAATGCCCAAAGACATTCAGCTCGCTCGTCGTATCAGAGGTGAACGCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05202 ko05322 map05034 map05202 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

136

Amino Acids

15.41

Weight (kDa)

11.15

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 44 - 131 1e-38 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 98
AccBSI CCGCTC 1 cut(s) 23
AccII CGCG 1 cut(s) 405
AciI CCGC 1 cut(s) 21
AfaI GTAC 3 cut(s) 10, 164, 175
AfiI CCNNNNNNNGG 1 cut(s) 98
AgsI TTSAA 2 cut(s) 238, 317
AjnI CCWGG 2 cut(s) 130, 279
AluBI AGCT 4 cut(s) 47, 275, 287, 379
AluI AGCT 4 cut(s) 47, 275, 287, 379
Alw26I GTCTC 1 cut(s) 314
ApeKI GCWGC 2 cut(s) 74, 287
AspLEI GCGC 1 cut(s) 407
AsuHPI GGTGA 1 cut(s) 410
BbsI GAAGAC 1 cut(s) 324
BbvI GCAGC 2 cut(s) 61, 274
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 2 cut(s) 132, 281
BcoDI GTCTC 1 cut(s) 314
BfaI CTAG 1 cut(s) 212
BfmI CTRYAG 1 cut(s) 288
BisI GCNGC 2 cut(s) 75, 288
BlsI GCNGC 2 cut(s) 76, 289
BmcAI AGTACT 1 cut(s) 175
Bme1390I CCNGG 2 cut(s) 132, 281
BmrFI CCNGG 2 cut(s) 132, 281
BpiI GAAGAC 1 cut(s) 324
BpmI CTGGAG 1 cut(s) 263
BpuEI CTTGAG 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 314
BsaJI CCNNGG 1 cut(s) 50
BsaWI WCCGGW 1 cut(s) 35
Bsc4I CCNNNNNNNGG 1 cut(s) 98
Bse1I ACTGG 1 cut(s) 103
BseBI CCWGG 2 cut(s) 132, 281
BseDI CCNNGG 1 cut(s) 50
BseLI CCNNNNNNNGG 1 cut(s) 98
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 103
BseXI GCAGC 2 cut(s) 61, 274
BsgI GTGCAG 1 cut(s) 72
Bsh1236I CGCG 1 cut(s) 405
BsiSI CCGG 1 cut(s) 36
BslI CCNNNNNNNGG 1 cut(s) 98
BsmAI GTCTC 1 cut(s) 314
Bso31I GGTCTC 1 cut(s) 314
BspACI CCGC 1 cut(s) 21
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 1 cut(s) 405
BspMAI CTGCAG 1 cut(s) 292
BspTNI GGTCTC 1 cut(s) 314
BsrBI CCGCTC 1 cut(s) 23
BsrI ACTGG 1 cut(s) 103
BssECI CCNNGG 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 50
Bst2UI CCWGG 2 cut(s) 132, 281
Bst4CI ACNGT 2 cut(s) 128, 139
Bst6I CTCTTC 1 cut(s) 164
BstC8I GCNNGC 1 cut(s) 381
BstDEI CTNAG 3 cut(s) 69, 177, 271
BstFNI CGCG 1 cut(s) 405
BstHHI GCGC 1 cut(s) 407
BstMAI GTCTC 1 cut(s) 314
BstMWI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
BstNI CCWGG 2 cut(s) 132, 281
BstSCI CCNGG 2 cut(s) 130, 279
BstSFI CTRYAG 1 cut(s) 288
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 2 cut(s) 61, 274
BstV2I GAAGAC 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 381
CfoI GCGC 1 cut(s) 407
Csp6I GTAC 3 cut(s) 9, 163, 174
CviAII CATG 2 cut(s) 263, 341
CviJI RGCY 8 cut(s) 5, 47, 74, 211, 261, 275, 287, 379
CviKI_1 RGCY 8 cut(s) 5, 47, 74, 211, 261, 275, 287, 379
CviQI GTAC 3 cut(s) 9, 163, 174
DdeI CTNAG 3 cut(s) 69, 177, 271
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
Eco130I CCWWGG 1 cut(s) 50
Eco31I GGTCTC 1 cut(s) 314
EcoRII CCWGG 2 cut(s) 130, 279
EcoT14I CCWWGG 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 2 cut(s) 266, 344
FaiI YATR 4 cut(s) 264, 298, 342, 359
FalI AAGNNNNNCTT 2 cut(s) 285, 317
FatI CATG 2 cut(s) 262, 340
Fnu4HI GCNGC 2 cut(s) 75, 288
Fsp4HI GCNGC 2 cut(s) 75, 288
FspBI CTAG 1 cut(s) 212
GlaI GCGC 1 cut(s) 406
GluI GCNGC 2 cut(s) 75, 288
GsuI CTGGAG 1 cut(s) 263
HapII CCGG 1 cut(s) 36
HhaI GCGC 1 cut(s) 407
Hin1II CATG 2 cut(s) 266, 344
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HinfI GANTC 1 cut(s) 105
HpaII CCGG 1 cut(s) 36
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 2 cut(s) 33, 401
Hpy188I TCNGA 2 cut(s) 207, 395
Hpy188III TCNNGA 5 cut(s) 109, 190, 230, 238, 256
Hpy8I GTNNAC 2 cut(s) 33, 401
Hpy99I CGWCG 1 cut(s) 390
HpyCH4III ACNGT 2 cut(s) 128, 139
HpyCH4V TGCA 2 cut(s) 89, 290
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
HpyF3I CTNAG 3 cut(s) 69, 177, 271
Hsp92II CATG 2 cut(s) 266, 344
HspAI GCGC 1 cut(s) 405
LmnI GCTCC 1 cut(s) 52
LpnPI CCDG 9 cut(s) 49, 84, 117, 144, 175, 179, 266, 269, 293
Lsp1109I GCAGC 2 cut(s) 61, 274
MaeI CTAG 1 cut(s) 212
MaeIII GTNAC 2 cut(s) 122, 352
MbiI CCGCTC 1 cut(s) 23
MboII GAAGA 2 cut(s) 181, 329
MlyI GAGTC 1 cut(s) 114
MnlI CCTC 3 cut(s) 32, 201, 389
MseI TTAA 1 cut(s) 409
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 2 cut(s) 132, 281
MvaI CCWGG 2 cut(s) 132, 281
MvnI CGCG 1 cut(s) 405
MwoI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
NlaIII CATG 2 cut(s) 266, 344
NmuCI GTSAC 1 cut(s) 352
PflMI CCANNNNNTGG 1 cut(s) 98
PfoI TCCNGGA 2 cut(s) 130, 279
PkrI GCNGC 2 cut(s) 76, 289
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
Psp6I CCWGG 2 cut(s) 130, 279
PspGI CCWGG 2 cut(s) 130, 279
PstI CTGCAG 1 cut(s) 292
RsaI GTAC 3 cut(s) 10, 164, 175
RsaNI GTAC 3 cut(s) 9, 163, 174
SaqAI TTAA 1 cut(s) 409
SatI GCNGC 2 cut(s) 75, 288
ScaI AGTACT 1 cut(s) 175
SchI GAGTC 1 cut(s) 114
ScrFI CCNGG 2 cut(s) 132, 281
SetI ASST 7 cut(s) 43, 49, 277, 289, 330, 381, 400
SfcI CTRYAG 1 cut(s) 288
SmlI CTYRAG 1 cut(s) 228
SmoI CTYRAG 1 cut(s) 228
SsiI CCGC 1 cut(s) 21
SspMI CTAG 1 cut(s) 212
StyD4I CCNGG 2 cut(s) 130, 279
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 2 cut(s) 128, 139
TatI WGTACW 1 cut(s) 173
Tru1I TTAA 1 cut(s) 409
Tru9I TTAA 1 cut(s) 409
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 2 cut(s) 74, 287
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 1 cut(s) 329
TspGWI ACGGA 3 cut(s) 137, 146, 206
Van91I CCANNNNNTGG 1 cut(s) 98
XspI CTAG 1 cut(s) 212
ZrmI AGTACT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.