MD01G1035300.v1.1

histone H3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001010
Physical Location & Seq
Reverse (-)
11967731 .. 11969419
1689 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1035300.v1.1.491

Sequence Viewer

Length: 549 bp
ATGGCATCCGTCCGATCCCCCAGCCACTTTACAATCACACCCTATATATACGCCTTAACCTGTCTAACGTTGGAAACTTTTGTTCGTTCGAAGAAGGAGAAGACCGAGACGAACAGAGCGCGAATCAAGCAGAGGTATATGGCCCGTACCAAGCAAACTGCTCGTAAGTCAACTGGAGGAAAGGCTCCCAGGAAGCAGCTCGCAACCAAGGCTGCCCGTAAGTCTGCACCAACCACTGGAGGAGTCAAGAAGCCCCACAGATACCGCCCGGGTACCGTTGCTCTTCGTGAAATCCGTAAGTACCAGAAGAGTACTGAGCTGTTGATCAGGAAGCTGCCATTCCAGAGGCTTGTTCGTGAAATTGCCCAGGACTTCAAGACTGATCTGCGTTTCCAGAGCCATGCAGTGCTGGCACTGCAGGAGGCTGCTGAGGCTTACCTGGTGGGTCTGTTTGAGGACACCAACCTTTGCGCTATCCATGCCAAGCGGGTTACCATCATGCCGAAGGATATCCAGCTTGCCAGGAGGATCAGGGGTGAGCGTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05202 ko05322 map05034 map05202 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

183

Amino Acids

20.83

Weight (kDa)

10.91

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 90 - 177 2.3e-38 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 272
AccB1I GGYRCC 1 cut(s) 272
AccB7I CCANNNNNTGG 1 cut(s) 236
AccII CGCG 1 cut(s) 121
AciI CCGC 2 cut(s) 265, 487
AclI AACGTT 1 cut(s) 68
AclWI GGATC 2 cut(s) 9, 536
AfaI GTAC 4 cut(s) 148, 274, 302, 313
AfiI CCNNNNNNNGG 1 cut(s) 236
AgsI TTSAA 1 cut(s) 376
AjnI CCWGG 4 cut(s) 188, 366, 438, 521
AluBI AGCT 4 cut(s) 199, 319, 334, 517
AluI AGCT 4 cut(s) 199, 319, 334, 517
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 2 cut(s) 9, 536
Ama87I CYCGRG 1 cut(s) 268
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 4 cut(s) 196, 212, 334, 425
Asp718I GGTACC 1 cut(s) 272
AspLEI GCGC 2 cut(s) 121, 473
AspS9I GGNCC 1 cut(s) 142
AsuC2I CCSGG 2 cut(s) 269, 270
AsuHPI GGTGA 1 cut(s) 548
AsuII TTCGAA 1 cut(s) 89
AvaI CYCGRG 1 cut(s) 268
BanI GGYRCC 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 107
BbvCI CCTCAGC 1 cut(s) 429
BbvI GCAGC 4 cut(s) 199, 208, 321, 412
BccI CCATC 1 cut(s) 503
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 4 cut(s) 190, 368, 440, 523
BclI TGATCA 1 cut(s) 324
BcnI CCSGG 2 cut(s) 269, 270
BcoDI GTCTC 1 cut(s) 101
BfmI CTRYAG 1 cut(s) 416
BisI GCNGC 4 cut(s) 197, 213, 335, 426
BlsI GCNGC 4 cut(s) 198, 214, 336, 427
BmcAI AGTACT 1 cut(s) 313
Bme1390I CCNGG 6 cut(s) 190, 269, 270, 368, 440, 523
BmeT110I CYCGRG 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 142
BmiI GGNNCC 2 cut(s) 186, 274
BmrFI CCNGG 6 cut(s) 190, 269, 270, 368, 440, 523
BmsI GCATC 1 cut(s) 14
BpiI GAAGAC 1 cut(s) 107
BpmI CTGGAG 2 cut(s) 195, 258
Bpu10I CCTNAGC 1 cut(s) 429
Bpu14I TTCGAA 1 cut(s) 89
BpuMI CCSGG 2 cut(s) 269, 270
BsaJI CCNNGG 4 cut(s) 188, 207, 268, 366
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse1I ACTGG 2 cut(s) 178, 241
BseBI CCWGG 4 cut(s) 190, 368, 440, 523
BseDI CCNNGG 4 cut(s) 188, 207, 268, 366
BseGI GGATG 1 cut(s) 5
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMII CTCAG 2 cut(s) 306, 420
BseNI ACTGG 2 cut(s) 178, 241
BseRI GAGGAG 1 cut(s) 255
BseXI GCAGC 4 cut(s) 199, 208, 321, 412
BseYI CCCAGC 1 cut(s) 20
BsgI GTGCAG 1 cut(s) 210
Bsh1236I CGCG 1 cut(s) 121
BshFI GGCC 1 cut(s) 143
BshNI GGYRCC 1 cut(s) 272
BsiHKCI CYCGRG 1 cut(s) 268
BsiSI CCGG 1 cut(s) 269
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 1 cut(s) 101
BsmBI CGTCTC 1 cut(s) 101
BsnI GGCC 1 cut(s) 143
BsoBI CYCGRG 1 cut(s) 268
Bsp119I TTCGAA 1 cut(s) 89
Bsp143I GATC 4 cut(s) 14, 324, 382, 528
BspACI CCGC 2 cut(s) 265, 487
BspANI GGCC 1 cut(s) 143
BspCNI CTCAG 2 cut(s) 307, 421
BspFNI CGCG 1 cut(s) 121
BspLI GGNNCC 2 cut(s) 186, 274
BspMAI CTGCAG 1 cut(s) 420
BspPI GGATC 2 cut(s) 9, 536
BspQI GCTCTTC 1 cut(s) 288
BspT104I TTCGAA 1 cut(s) 89
BspT107I GGYRCC 1 cut(s) 272
BsrI ACTGG 2 cut(s) 178, 241
BssECI CCNNGG 4 cut(s) 188, 207, 268, 366
BssMI GATC 4 cut(s) 14, 324, 382, 528
BssT1I CCWWGG 1 cut(s) 207
Bst2UI CCWGG 4 cut(s) 190, 368, 440, 523
Bst4CI ACNGT 1 cut(s) 277
Bst6I CTCTTC 2 cut(s) 288, 302
BstBI TTCGAA 1 cut(s) 89
BstC8I GCNNGC 4 cut(s) 201, 411, 519, 543
BstDEI CTNAG 2 cut(s) 315, 429
BstEII GGTNACC 1 cut(s) 490
BstF5I GGATG 1 cut(s) 5
BstFNI CGCG 1 cut(s) 121
BstHHI GCGC 2 cut(s) 121, 473
BstKTI GATC 4 cut(s) 17, 327, 385, 531
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 4 cut(s) 14, 324, 382, 528
BstMWI GCNNNNNNNGC 6 cut(s) 127, 209, 410, 415, 431, 479
BstNI CCWGG 4 cut(s) 190, 368, 440, 523
BstPI GGTNACC 1 cut(s) 490
BstSCI CCNGG 6 cut(s) 188, 267, 268, 366, 438, 521
BstSFI CTRYAG 1 cut(s) 416
BstUI CGCG 1 cut(s) 121
BstV1I GCAGC 4 cut(s) 199, 208, 321, 412
BstV2I GAAGAC 1 cut(s) 107
BsuRI GGCC 1 cut(s) 143
BtsCI GGATG 1 cut(s) 5
BtsI GCAGTG 2 cut(s) 411, 413
BtsIMutI CAGTG 3 cut(s) 234, 411, 413
Cac8I GCNNGC 4 cut(s) 201, 411, 519, 543
CfoI GCGC 2 cut(s) 121, 473
Cfr13I GGNCC 1 cut(s) 142
Cfr9I CCCGGG 1 cut(s) 268
CsiI ACCWGGT 1 cut(s) 438
Csp6I GTAC 4 cut(s) 147, 273, 301, 312
CviAII CATG 3 cut(s) 401, 479, 499
CviQI GTAC 4 cut(s) 147, 273, 301, 312
DdeI CTNAG 2 cut(s) 315, 429
DpnI GATC 4 cut(s) 16, 326, 384, 530
DpnII GATC 4 cut(s) 14, 324, 382, 528
Eam1104I CTCTTC 2 cut(s) 288, 302
EarI CTCTTC 2 cut(s) 288, 302
Eco130I CCWWGG 1 cut(s) 207
Eco32I GATATC 1 cut(s) 511
Eco88I CYCGRG 1 cut(s) 268
Eco91I GGTNACC 1 cut(s) 490
EcoO65I GGTNACC 1 cut(s) 490
EcoRII CCWGG 4 cut(s) 188, 366, 438, 521
EcoRV GATATC 1 cut(s) 511
EcoT14I CCWWGG 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 207
Esp3I CGTCTC 1 cut(s) 101
FaeI CATG 3 cut(s) 404, 482, 502
FaiI YATR 8 cut(s) 45, 47, 49, 138, 140, 402, 480, 500
FatI CATG 3 cut(s) 400, 478, 498
FauI CCCGC 1 cut(s) 480
FbaI TGATCA 1 cut(s) 324
Fnu4HI GCNGC 4 cut(s) 197, 213, 335, 426
Fsp4HI GCNGC 4 cut(s) 197, 213, 335, 426
GlaI GCGC 2 cut(s) 120, 472
GluI GCNGC 4 cut(s) 197, 213, 335, 426
GsaI CCCAGC 1 cut(s) 24
GsuI CTGGAG 2 cut(s) 195, 258
HaeIII GGCC 1 cut(s) 143
HapII CCGG 1 cut(s) 269
HhaI GCGC 2 cut(s) 121, 473
Hin1II CATG 3 cut(s) 404, 482, 502
Hin6I GCGC 2 cut(s) 119, 471
HinP1I GCGC 2 cut(s) 119, 471
HincII GTYRAC 1 cut(s) 171
HindII GTYRAC 1 cut(s) 171
HinfI GANTC 2 cut(s) 123, 243
HpaII CCGG 1 cut(s) 269
HphI GGTGA 1 cut(s) 548
Hpy166II GTNNAC 1 cut(s) 171
Hpy188I TCNGA 1 cut(s) 14
Hpy188III TCNNGA 7 cut(s) 247, 287, 328, 343, 356, 376, 394
Hpy8I GTNNAC 1 cut(s) 171
HpyAV CCTTC 2 cut(s) 88, 499
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4IV ACGT 1 cut(s) 68
HpyCH4V TGCA 3 cut(s) 227, 404, 418
HpyF10VI GCNNNNNNNGC 6 cut(s) 127, 209, 410, 415, 431, 479
HpyF3I CTNAG 2 cut(s) 315, 429
HpySE526I ACGT 1 cut(s) 68
Hsp92II CATG 3 cut(s) 404, 482, 502
HspAI GCGC 2 cut(s) 119, 471
KpnI GGTACC 1 cut(s) 276
Ksp22I TGATCA 1 cut(s) 324
Kzo9I GATC 4 cut(s) 14, 324, 382, 528
LguI GCTCTTC 1 cut(s) 288
LmnI GCTCC 1 cut(s) 190
Lsp1109I GCAGC 4 cut(s) 199, 208, 321, 412
LweI GCATC 1 cut(s) 14
MabI ACCWGGT 1 cut(s) 438
MaeII ACGT 1 cut(s) 68
MaeIII GTNAC 1 cut(s) 490
MalI GATC 4 cut(s) 16, 326, 384, 530
MboI GATC 4 cut(s) 14, 324, 382, 528
MboII GAAGA 4 cut(s) 103, 112, 275, 319
MluCI AATT 1 cut(s) 360
MlyI GAGTC 1 cut(s) 252
MmeI TCCRAC 1 cut(s) 51
MnlI CCTC 8 cut(s) 126, 170, 233, 339, 415, 424, 448, 519
MseI TTAA 1 cut(s) 56
MspI CCGG 1 cut(s) 269
MspR9I CCNGG 6 cut(s) 190, 269, 270, 368, 440, 523
MvaI CCWGG 4 cut(s) 190, 368, 440, 523
MvnI CGCG 1 cut(s) 121
MwoI GCNNNNNNNGC 6 cut(s) 127, 209, 410, 415, 431, 479
NciI CCSGG 2 cut(s) 269, 270
NdeII GATC 4 cut(s) 14, 324, 382, 528
NlaIII CATG 3 cut(s) 404, 482, 502
NlaIV GGNNCC 2 cut(s) 186, 274
NspV TTCGAA 1 cut(s) 89
PciSI GCTCTTC 1 cut(s) 288
PcsI WCGNNNNNNNCGW 1 cut(s) 292
PfeI GAWTC 1 cut(s) 123
PflMI CCANNNNNTGG 1 cut(s) 236
PkrI GCNGC 4 cut(s) 198, 214, 336, 427
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
Psp1406I AACGTT 1 cut(s) 68
Psp6I CCWGG 4 cut(s) 188, 366, 438, 521
PspEI GGTNACC 1 cut(s) 490
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 4 cut(s) 188, 366, 438, 521
PspN4I GGNNCC 2 cut(s) 186, 274
PspPI GGNCC 1 cut(s) 142
PstI CTGCAG 1 cut(s) 420
RsaI GTAC 4 cut(s) 148, 274, 302, 313
RsaNI GTAC 4 cut(s) 147, 273, 301, 312
SapI GCTCTTC 1 cut(s) 288
SaqAI TTAA 1 cut(s) 56
SatI GCNGC 4 cut(s) 197, 213, 335, 426
Sau3AI GATC 4 cut(s) 14, 324, 382, 528
Sau96I GGNCC 1 cut(s) 142
ScaI AGTACT 1 cut(s) 313
SchI GAGTC 1 cut(s) 252
ScrFI CCNGG 6 cut(s) 190, 269, 270, 368, 440, 523
SetI ASST 9 cut(s) 62, 71, 137, 201, 321, 336, 441, 468, 519
SexAI ACCWGGT 1 cut(s) 438
SfaNI GCATC 1 cut(s) 14
SfcI CTRYAG 1 cut(s) 416
SfuI TTCGAA 1 cut(s) 89
SmaI CCCGGG 1 cut(s) 270
Sse9I AATT 1 cut(s) 360
SsiI CCGC 2 cut(s) 265, 487
StyD4I CCNGG 6 cut(s) 188, 267, 268, 366, 438, 521
StyI CCWWGG 1 cut(s) 207
TaaI ACNGT 1 cut(s) 277
TaiI ACGT 1 cut(s) 71
TaqI TCGA 1 cut(s) 89
TaqII GACCGA 1 cut(s) 119
TasI AATT 1 cut(s) 360
TatI WGTACW 1 cut(s) 311
TfiI GAWTC 1 cut(s) 123
Tru1I TTAA 1 cut(s) 56
Tru9I TTAA 1 cut(s) 56
TscAI CASTG 3 cut(s) 241, 411, 420
TseI GCWGC 4 cut(s) 196, 212, 334, 425
TspGWI ACGGA 1 cut(s) 284
TspMI CCCGGG 1 cut(s) 268
TspRI CASTG 3 cut(s) 241, 411, 420
Van91I CCANNNNNTGG 1 cut(s) 236
XmaI CCCGGG 1 cut(s) 268
ZrmI AGTACT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.