Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000007

Orthogroup ID

OG0002789

Physical Location & Seq

Genomic Coordinates Reverse (-)

37814485 .. 37819286

Gene Structure 4802 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug07G0342900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1593 bp

ATGCAGGCTTGCAGTGGAGCTGCAGTTATGGGTTCTGTTCAACAGCCCACATGGCTTAGAGGAGCAGCTTTTCCAAATAGACGACTTAGCATTAGTGGATTTCCACATCAAGTGAACCTTAATTTTGCAAAGACTGCTAGATCTTATTCTAACATCCAAGGAAGCTTGGTTGCTGGGAGGCCATCCTCTCCAGCTTCAGTGCATGTGCCTGAATTTGGAGATATTGGAAGCAGTTTTGTAGATAATGGCTTAAGTGAGGCTGACCCTGAGGTGCGTGCTATTATTGACAAGGAGAAAGAGCGGCAGTTCAAAAGCCTTGAGCTTATTGCCTCAGAGAATTTCACTTCACGGGCAGTAATGGAGGCAGTTGGCTCATGTCTCACAAACAAGTATTCGGAAGGATTACCTGGGAAAAGGTACTATGGTGGAAATGAGCACATTGATGAGCTTGAAACACTATGCCAAAAAAGGGCCCTGGATGCATTTCACCTAGATGGAAAGAAGTGGGGCGTCAATGTTCAACCATTATCTGGTTCTCCAGCTAATTTTGAGGTTTATACAGCAGTTCTCAATCCTCATGATCGTATCATGGGTTTGGACTTGCCACACGGGGGACATTTGTCTCATGGGTTCATGACTCCTAAAAGACGGGTATCAGGAACATCTATTTATTTTGAGTCCATGCCTTACCGACTTGATGAATCCACAGGTCTGGTTGATTATGACATGCTTGAGAAAACAGCAACCCTATTTCGACCTAAACTCATTATTGTTGGGGCCAGTGCTTATCCTCGAGATTTTGACTATCCTCGCATGAGGAAGATTGCGGATGCTGCTGGTGCTTTTCTTATGATGGATATGGCTCACATAAGTGGGCTTGTTGCTGCTTCTGTGGTTGCCAATCCCTTTGACTACTGTGATATTGTGACAACAACAACCCACAAGTCTCTAAGGGGTCCAAGAGGTGGAATGATATTCTTCAAGAAGGATCCAGTTCTTGGAGTTGATTTAGAAACTGCCATCAACAACGCTGTATTCCCGGGCTTACAGGGTGGTCCTCATAACCACACTATTGGGGGCCTTGCAGTTTGCTTGAAGCATGCTCAGTCCGCAGAATTTAAGGCTTACCAGAATCAGGTGGTCTCTAATTGCAGGGCTCTTGCAAGCCGATTGGTTGAACTTGAATACACACTGGTTTCTGGTGGAAGCGATAACCACCTGGTTCTTGTCGATCTGCGTCCCTTTGGCATTGATGGTGCTCGGGTGGAGAAAATTCTTGACATGGCTTCTATTACTCTTAACAAGAATTCAGTACCCGGTGATAAAAGTGCTCTAATGCCTGGTGGTATTCGCATTGGAACACCTGCTATGACGACACGAGGATTCACTGAGAAAGACTTCATAGCAACTGCAGACTACATTCACGAGGGTGTGCGGATAGCGATTGATGCTAAAAGCAAAGCCCCGGGCTCAAAGCTCCAGGAGTTTTTAAAGCATGTTGCAACCCCTGAATTCCCTCTAATTGATCGAGTTTCAGATCTGCGGACAAAAGTCGAAGCTCTTACCACCCAATTCCCGATGCCTGGAGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000105 GO:0003674 GO:0003824 GO:0004399 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006547 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009314 GO:0009411 GO:0009416 GO:0009507 GO:0009532 GO:0009536 GO:0009555 GO:0009570 GO:0009628 GO:0009987 GO:0016053 GO:0016491 GO:0016614 GO:0016616 GO:0018130 GO:0019438 GO:0019752 GO:0032501 GO:0032502 GO:0034641 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046483 GO:0048229 GO:0048856 GO:0050896 GO:0052803 GO:0055114 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230

Pfam Domains

Protein Families

Histidinol_dh

Protein Analysis

530

Amino Acids

57.59

Weight (kDa)

7.27

Isoelectric Point (pI)

33.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SHMT	PF00464	84 - 474	3.1e-191	Serine hydroxymethyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002789)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63890 AT5G63890
fragaria_vesca	FvH4_3g10270 FvH4_3g10270 FvH4_3g10270 FvH4_3g10270 FvH4_3g10270 FvH4_3g10270 FvH4_3g10270
malus_domestica	MD05G1271100.v1.1 MD10G1251700.v1.1
prunus_persica	Prupe.4G091700_v2.0.a1 Prupe.4G091700_v2.0.a1 Prupe.4G091700_v2.0.a1 Prupe.4G091700_v2.0.a1 Prupe.4G091700_v2.0.a1
pyrus_communis	pycom05g25350 pycom10g20760
rosa_chinensis	RchiOBHm_Chr5g0016531 RchiOBHm_Chr7g0241131
rosa_laevigata	RLG00000000593
rosa_multiflora	Rmu_sc0006665.1_g000005 Rmu_sc0008150.1_g000003
rosa_roxburghii	Rroxscaffold_1G00060350 Rroxscaffold_3G00219240
rosa_rugosa	Rorug07G0342600 Rorug07G0342700 Rorug07G0342800 Rorug07G0342900
rosa_samantha	Rh5AG126900 Rh5BG125800 Rh5CG138500 Rh5DG126000 Rh7AG498000 Rh7BG468600 Rh7CG514700 Rh7DG482000
rosa_wichuraiana	Rw5G010990 Rw7G042000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1372
Acc36I	ACCTGC	1 cut(s)	1372
AccB7I	CCANNNNNTGG	3 cut(s)	530, 965, 998
AccBSI	CCGCTC	1 cut(s)	301
AciI	CCGC	5 cut(s)	301, 827, 1110, 1435, 1543
AclWI	GGATC	2 cut(s)	983, 996
AcsI	RAATTY	6 cut(s)	212, 337, 1115, 1272, 1306, 1511
AcuI	CTGAAG	1 cut(s)	180
AcyI	GRCGYC	1 cut(s)	510
AfaI	GTAC	2 cut(s)	419, 1314
AfiI	CCNNNNNNNGG	8 cut(s)	215, 469, 530, 611, 965, 998, 1135, 1583
AflII	CTTAAG	1 cut(s)	250
AgsI	TTSAA	8 cut(s)	41, 310, 452, 521, 982, 1096, 1178, 1184
AjnI	CCWGG	6 cut(s)	406, 474, 1218, 1339, 1479, 1582
AleI	CACNNNNGTG	2 cut(s)	870, 1428
AluBI	AGCT	9 cut(s)	20, 68, 165, 194, 322, 448, 542, 1477, 1559
AluI	AGCT	9 cut(s)	20, 68, 165, 194, 322, 448, 542, 1477, 1559
Alw21I	GWGCWC	3 cut(s)	438, 1261, 1333
Alw26I	GTCTC	4 cut(s)	383, 627, 951, 1147
AlwI	GGATC	2 cut(s)	983, 996
Ama87I	CYCGRG	4 cut(s)	792, 1039, 1260, 1465
AoxI	GGCC	4 cut(s)	179, 471, 777, 1078
ApaI	GGGCCC	1 cut(s)	475
ApeKI	GCWGC	4 cut(s)	20, 65, 833, 884
ApoI	RAATTY	6 cut(s)	212, 337, 1115, 1272, 1306, 1511
Asp700I	GAANNNNTTC	1 cut(s)	1397
AspS9I	GGNCC	6 cut(s)	471, 472, 777, 956, 1055, 1078
AsuC2I	CCSGG	5 cut(s)	1040, 1041, 1317, 1466, 1467
AsuHPI	GGTGA	2 cut(s)	479, 1331
AvaI	CYCGRG	4 cut(s)	792, 1039, 1260, 1465
AvaII	GGWCC	2 cut(s)	956, 1055
AxyI	CCTNAGG	1 cut(s)	267
BaeGI	GKGCMC	1 cut(s)	475
BaeI	ACNNNNGTAYC	2 cut(s)	409, 442
BamHI	GGATCC	1 cut(s)	988
BanII	GRGCYC	3 cut(s)	475, 1159, 1472
BauI	CACGAG	2 cut(s)	1377, 1424
Bbv12I	GWGCWC	3 cut(s)	438, 1261, 1333
BbvI	GCAGC	4 cut(s)	7, 77, 820, 871
BccI	CCATC	5 cut(s)	190, 488, 847, 1028, 1247
BciT130I	CCWGG	6 cut(s)	408, 476, 1220, 1341, 1481, 1584
BcnI	CCSGG	5 cut(s)	1040, 1041, 1317, 1466, 1467
BcoDI	GTCTC	4 cut(s)	383, 627, 951, 1147
BfaI	CTAG	2 cut(s)	138, 491
BfmI	CTRYAG	2 cut(s)	21, 1410
BfrI	CTTAAG	1 cut(s)	250
BfuAI	ACCTGC	1 cut(s)	1372
BglI	GCCNNNNNGGC	1 cut(s)	52
BglII	AGATCT	2 cut(s)	140, 1537
BisI	GCNGC	5 cut(s)	21, 66, 302, 834, 885
BlsI	GCNGC	5 cut(s)	22, 67, 303, 835, 886
Bme18I	GGWCC	2 cut(s)	956, 1055
BmeT110I	CYCGRG	4 cut(s)	792, 1039, 1260, 1465
BmgT120I	GGNCC	6 cut(s)	471, 472, 777, 956, 1055, 1078
BmiI	GGNNCC	5 cut(s)	473, 778, 957, 990, 1079
BmsI	GCATC	4 cut(s)	469, 820, 1438, 1569
BoxI	GACNNNNGTC	2 cut(s)	619, 1550
BpmI	CTGGAG	3 cut(s)	174, 522, 1463
BpuEI	CTTGAG	2 cut(s)	338, 752
BpuMI	CCSGG	5 cut(s)	1040, 1041, 1317, 1466, 1467
BsaHI	GRCGYC	1 cut(s)	510
BsaI	GGTCTC	1 cut(s)	1147
BsaJI	CCNNGG	6 cut(s)	157, 407, 474, 1039, 1464, 1465
BsaXI	ACNNNNNCTCC	2 cut(s)	9, 39
Bsc4I	CCNNNNNNNGG	8 cut(s)	215, 469, 530, 611, 965, 998, 1135, 1583
Bse1I	ACTGG	3 cut(s)	780, 992, 1197
Bse21I	CCTNAGG	1 cut(s)	267
BseBI	CCWGG	6 cut(s)	408, 476, 1220, 1341, 1481, 1584
BseDI	CCNNGG	6 cut(s)	157, 407, 474, 1039, 1464, 1465
BseGI	GGATG	4 cut(s)	153, 182, 484, 835
BseLI	CCNNNNNNNGG	8 cut(s)	215, 469, 530, 611, 965, 998, 1135, 1583
BseMII	CTCAG	4 cut(s)	258, 345, 1118, 1380
BseNI	ACTGG	3 cut(s)	780, 992, 1197
BseRI	GAGGAG	1 cut(s)	75
BseSI	GKGCMC	1 cut(s)	475
BseXI	GCAGC	4 cut(s)	7, 77, 820, 871
BseYI	CCCAGC	1 cut(s)	173
BshFI	GGCC	4 cut(s)	181, 473, 779, 1080
BsiHKAI	GWGCWC	3 cut(s)	438, 1261, 1333
BsiHKCI	CYCGRG	4 cut(s)	792, 1039, 1260, 1465
BsiSI	CCGG	3 cut(s)	1040, 1317, 1466
BslFI	GGGAC	2 cut(s)	627, 1224
BslI	CCNNNNNNNGG	8 cut(s)	215, 469, 530, 611, 965, 998, 1135, 1583
BsmAI	GTCTC	4 cut(s)	383, 627, 951, 1147
BsmFI	GGGAC	2 cut(s)	627, 1224
BsnI	GGCC	4 cut(s)	181, 473, 779, 1080
Bso31I	GGTCTC	1 cut(s)	1147
BsoBI	CYCGRG	4 cut(s)	792, 1039, 1260, 1465
Bsp120I	GGGCCC	1 cut(s)	471
Bsp1286I	GDGCHC	6 cut(s)	438, 475, 1159, 1261, 1333, 1472
Bsp143I	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
BspACI	CCGC	5 cut(s)	301, 827, 1110, 1435, 1543
BspANI	GGCC	4 cut(s)	181, 473, 779, 1080
BspCNI	CTCAG	4 cut(s)	259, 344, 1117, 1381
BspHI	TCATGA	2 cut(s)	577, 633
BspLI	GGNNCC	5 cut(s)	473, 778, 957, 990, 1079
BspMAI	CTGCAG	2 cut(s)	25, 1414
BspMI	ACCTGC	1 cut(s)	1372
BspPI	GGATC	2 cut(s)	983, 996
BspTI	CTTAAG	1 cut(s)	250
BspTNI	GGTCTC	1 cut(s)	1147
BsrBI	CCGCTC	1 cut(s)	301
BsrI	ACTGG	3 cut(s)	780, 992, 1197
BssECI	CCNNGG	6 cut(s)	157, 407, 474, 1039, 1464, 1465
BssMI	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
BssNI	GRCGYC	1 cut(s)	510
BssSI	CACGAG	2 cut(s)	1377, 1424
BssT1I	CCWWGG	1 cut(s)	157
Bst2BI	CACGAG	2 cut(s)	1377, 1424
Bst2UI	CCWGG	6 cut(s)	408, 476, 1220, 1341, 1481, 1584
Bst4CI	ACNGT	1 cut(s)	917
BstACI	GRCGYC	1 cut(s)	510
BstAFI	CTTAAG	1 cut(s)	250
BstAPI	GCANNNNNTGC	1 cut(s)	134
BstC8I	GCNNGC	5 cut(s)	6, 10, 276, 1101, 1165
BstDEI	CTNAG	7 cut(s)	56, 86, 267, 331, 950, 1104, 1389
BstF5I	GGATG	4 cut(s)	153, 182, 484, 835
BstKTI	GATC	6 cut(s)	143, 583, 991, 1234, 1528, 1540
BstMAI	GTCTC	4 cut(s)	383, 627, 951, 1147
BstMBI	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
BstMWI	GCNNNNNNNGC	7 cut(s)	52, 134, 479, 833, 839, 1109, 1448
BstNI	CCWGG	6 cut(s)	408, 476, 1220, 1341, 1481, 1584
BstNSI	RCATGY	4 cut(s)	206, 730, 1103, 1499
BstPAI	GACNNNNGTC	2 cut(s)	619, 1550
BstSFI	CTRYAG	2 cut(s)	21, 1410
BstSLI	GKGCMC	1 cut(s)	475
BstV1I	GCAGC	4 cut(s)	7, 77, 820, 871
BstX2I	RGATCY	3 cut(s)	140, 988, 1537
BstXI	CCANNNNNNTGG	2 cut(s)	712, 1073
BstYI	RGATCY	3 cut(s)	140, 988, 1537
Bsu36I	CCTNAGG	1 cut(s)	267
BsuRI	GGCC	4 cut(s)	181, 473, 779, 1080
BtsCI	GGATG	4 cut(s)	153, 182, 484, 835
BtsI	GCAGTG	1 cut(s)	19
BtsIMutI	CAGTG	5 cut(s)	19, 204, 787, 1190, 1386
BveI	ACCTGC	1 cut(s)	1372
Cac8I	GCNNGC	5 cut(s)	6, 10, 276, 1101, 1165
CciI	TCATGA	2 cut(s)	577, 633
Cfr13I	GGNCC	6 cut(s)	471, 472, 777, 956, 1055, 1078
Cfr9I	CCCGGG	2 cut(s)	1039, 1465
CseI	GACGC	2 cut(s)	499, 1226
CsiI	ACCWGGT	1 cut(s)	1218
Csp6I	GTAC	2 cut(s)	418, 1313
CviQI	GTAC	2 cut(s)	418, 1313
DdeI	CTNAG	7 cut(s)	56, 86, 267, 331, 950, 1104, 1389
DpnI	GATC	6 cut(s)	142, 582, 990, 1233, 1527, 1539
DpnII	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
DraI	TTTAAA	1 cut(s)	1491
Eco130I	CCWWGG	1 cut(s)	157
Eco24I	GRGCYC	3 cut(s)	475, 1159, 1472
Eco31I	GGTCTC	1 cut(s)	1147
Eco47I	GGWCC	2 cut(s)	956, 1055
Eco57I	CTGAAG	1 cut(s)	180
Eco81I	CCTNAGG	1 cut(s)	267
Eco88I	CYCGRG	4 cut(s)	792, 1039, 1260, 1465
EcoO109I	RGGNCCY	3 cut(s)	471, 472, 1078
EcoRI	GAATTC	2 cut(s)	1306, 1511
EcoRII	CCWGG	6 cut(s)	406, 474, 1218, 1339, 1479, 1582
EcoT14I	CCWWGG	1 cut(s)	157
EcoT22I	ATGCAT	1 cut(s)	484
EcoT38I	GRGCYC	3 cut(s)	475, 1159, 1472
ErhI	CCWWGG	1 cut(s)	157
FalI	AAGNNNNNCTT	2 cut(s)	102, 134
FaqI	GGGAC	2 cut(s)	627, 1224
Fnu4HI	GCNGC	5 cut(s)	21, 66, 302, 834, 885
FokI	GGATG	4 cut(s)	140, 169, 491, 842
FriOI	GRGCYC	3 cut(s)	475, 1159, 1472
Fsp4HI	GCNGC	5 cut(s)	21, 66, 302, 834, 885
FspBI	CTAG	2 cut(s)	138, 491
GluI	GCNGC	5 cut(s)	21, 66, 302, 834, 885
GsaI	CCCAGC	1 cut(s)	177
GsuI	CTGGAG	3 cut(s)	174, 522, 1463
HaeIII	GGCC	4 cut(s)	181, 473, 779, 1080
HapII	CCGG	3 cut(s)	1040, 1317, 1466
HgaI	GACGC	2 cut(s)	499, 1226
Hin1I	GRCGYC	1 cut(s)	510
HindIII	AAGCTT	1 cut(s)	163
HinfI	GANTC	5 cut(s)	637, 677, 701, 1132, 1383
HpaII	CCGG	3 cut(s)	1040, 1317, 1466
HphI	GGTGA	2 cut(s)	479, 1331
Hpy166II	GTNNAC	1 cut(s)	115
Hpy188I	TCNGA	3 cut(s)	334, 397, 1537
Hpy188III	TCNNGA	8 cut(s)	578, 634, 657, 794, 982, 1277, 1424, 1576
Hpy8I	GTNNAC	1 cut(s)	115
HpyAV	CCTTC	2 cut(s)	392, 979
HpyCH4III	ACNGT	1 cut(s)	917
HpyF10VI	GCNNNNNNNGC	7 cut(s)	52, 134, 479, 833, 839, 1109, 1448
HpyF3I	CTNAG	7 cut(s)	56, 86, 267, 331, 950, 1104, 1389
Hsp92I	GRCGYC	1 cut(s)	510
Kzo9I	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
LmnI	GCTCC	3 cut(s)	17, 62, 1482
Lsp1109I	GCAGC	4 cut(s)	7, 77, 820, 871
LweI	GCATC	4 cut(s)	469, 820, 1438, 1569
MabI	ACCWGGT	1 cut(s)	1218
MaeI	CTAG	2 cut(s)	138, 491
MaeIII	GTNAC	1 cut(s)	925
MalI	GATC	6 cut(s)	142, 582, 990, 1233, 1527, 1539
MbiI	CCGCTC	1 cut(s)	301
MboI	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
MboII	GAAGA	2 cut(s)	832, 970
MflI	RGATCY	3 cut(s)	140, 988, 1537
MhlI	GDGCHC	6 cut(s)	438, 475, 1159, 1261, 1333, 1472
MlyI	GAGTC	2 cut(s)	631, 686
Mph1103I	ATGCAT	1 cut(s)	484
MroXI	GAANNNNTTC	1 cut(s)	1397
MseI	TTAA	5 cut(s)	120, 251, 1119, 1299, 1490
MslI	CAYNNNNRTG	4 cut(s)	441, 492, 870, 1428
MspCI	CTTAAG	1 cut(s)	250
MspI	CCGG	3 cut(s)	1040, 1317, 1466
MvaI	CCWGG	6 cut(s)	408, 476, 1220, 1341, 1481, 1584
MwoI	GCNNNNNNNGC	7 cut(s)	52, 134, 479, 833, 839, 1109, 1448
NciI	CCSGG	5 cut(s)	1040, 1041, 1317, 1466, 1467
NdeII	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
NlaIV	GGNNCC	5 cut(s)	473, 778, 957, 990, 1079
NmuCI	GTSAC	1 cut(s)	925
NsiI	ATGCAT	1 cut(s)	484
NspI	RCATGY	4 cut(s)	206, 730, 1103, 1499
OliI	CACNNNNGTG	2 cut(s)	870, 1428
PaeI	GCATGC	1 cut(s)	1103
PaeR7I	CTCGAG	1 cut(s)	792
PagI	TCATGA	2 cut(s)	577, 633
PaqCI	CACCTGC	1 cut(s)	1372
PdmI	GAANNNNTTC	1 cut(s)	1397
PfeI	GAWTC	3 cut(s)	701, 1132, 1383
PflMI	CCANNNNNTGG	3 cut(s)	530, 965, 998
PfoI	TCCNGGA	1 cut(s)	1479
PkrI	GCNGC	5 cut(s)	22, 67, 303, 835, 886
PleI	GAGTC	2 cut(s)	631, 685
PpsI	GAGTC	2 cut(s)	631, 685
PshAI	GACNNNNGTC	2 cut(s)	619, 1550
Psp6I	CCWGG	6 cut(s)	406, 474, 1218, 1339, 1479, 1582
PspFI	CCCAGC	1 cut(s)	173
PspGI	CCWGG	6 cut(s)	406, 474, 1218, 1339, 1479, 1582
PspN4I	GGNNCC	5 cut(s)	473, 778, 957, 990, 1079
PspOMI	GGGCCC	1 cut(s)	471
PspPI	GGNCC	6 cut(s)	471, 472, 777, 956, 1055, 1078
PstI	CTGCAG	2 cut(s)	25, 1414
PsuI	RGATCY	3 cut(s)	140, 988, 1537
RsaI	GTAC	2 cut(s)	419, 1314
RsaNI	GTAC	2 cut(s)	418, 1313
RseI	CAYNNNNRTG	4 cut(s)	441, 492, 870, 1428
SaqAI	TTAA	5 cut(s)	120, 251, 1119, 1299, 1490
SatI	GCNGC	5 cut(s)	21, 66, 302, 834, 885
Sau3AI	GATC	6 cut(s)	140, 580, 988, 1231, 1525, 1537
Sau96I	GGNCC	6 cut(s)	471, 472, 777, 956, 1055, 1078
SchI	GAGTC	2 cut(s)	631, 686
SduI	GDGCHC	6 cut(s)	438, 475, 1159, 1261, 1333, 1472
SexAI	ACCWGGT	1 cut(s)	1218
SfaNI	GCATC	4 cut(s)	469, 820, 1438, 1569
SfcI	CTRYAG	2 cut(s)	21, 1410
Sfr274I	CTCGAG	1 cut(s)	792
SinI	GGWCC	2 cut(s)	956, 1055
SlaI	CTCGAG	1 cut(s)	792
SmaI	CCCGGG	2 cut(s)	1041, 1467
SmiMI	CAYNNNNRTG	4 cut(s)	441, 492, 870, 1428
SmlI	CTYRAG	4 cut(s)	250, 317, 731, 792
SmoI	CTYRAG	4 cut(s)	250, 317, 731, 792
SphI	GCATGC	1 cut(s)	1103
SsiI	CCGC	5 cut(s)	301, 827, 1110, 1435, 1543
SspMI	CTAG	2 cut(s)	138, 491
StyI	CCWWGG	1 cut(s)	157
TaaI	ACNGT	1 cut(s)	917
TaqI	TCGA	5 cut(s)	754, 793, 1230, 1528, 1554
TauI	GCSGC	1 cut(s)	304
TfiI	GAWTC	3 cut(s)	701, 1132, 1383
Tru1I	TTAA	5 cut(s)	120, 251, 1119, 1299, 1490
Tru9I	TTAA	5 cut(s)	120, 251, 1119, 1299, 1490
TscAI	CASTG	5 cut(s)	19, 204, 787, 1197, 1393
TseFI	GTSAC	1 cut(s)	925
TseI	GCWGC	4 cut(s)	20, 65, 833, 884
Tsp45I	GTSAC	1 cut(s)	925
TspDTI	ATGAA	3 cut(s)	622, 714, 1390
TspMI	CCCGGG	2 cut(s)	1039, 1465
TspRI	CASTG	5 cut(s)	19, 204, 787, 1197, 1393
Van91I	CCANNNNNTGG	3 cut(s)	530, 965, 998
Vha464I	CTTAAG	1 cut(s)	250
VpaK11BI	GGWCC	2 cut(s)	956, 1055
XapI	RAATTY	6 cut(s)	212, 337, 1115, 1272, 1306, 1511
XceI	RCATGY	4 cut(s)	206, 730, 1103, 1499
XhoI	CTCGAG	1 cut(s)	792
XmaI	CCCGGG	2 cut(s)	1039, 1465
XmnI	GAANNNNTTC	1 cut(s)	1397
XspI	CTAG	2 cut(s)	138, 491
Zsp2I	ATGCAT	1 cut(s)	484

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.