Rh1AG225100

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0018004
Physical Location & Seq
Forward (+)
42800009 .. 42820809
20801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG225100.1

Sequence Viewer

Length: 1062 bp
ATGTGTATGTCCCCATTTTCCCCTCTAGAATGTCATTTGAATCATACGATACTTACTTTGATGAGTATATATATAACGAAGATAGCATCAGAAACATTCCCACAAAGCAGGCCATTGTGGGAACTTCATCTTTTCAAGTACCCAACAAGTCATGCAGCTGGACAAATAATATTCAAGATCCACCATGCCCTTGGCGATGGCTACTCTCTTATGGGCGCTTATCTCTCTTGTCTCCAAAATGCTCACAATCCTTCTCTTCCCCTGACATTTCCTTCACTAAAGGGTGCAAAGAATGAAACTAGTAGTCTATCTCGTGTGTTTGAGTTTGTGCCTAAGATACTTTCTGCCGTTATCAACGGTGCATGGGATTTCAGTTGGAGCATTTTGAAGGGCACTTGGGTTGAAGATGATCGAACACCAACAAGGTCCGGCGTTGTTGGAGTTGAGTTTCGGCCTGTGTCCATGTCGACCTTGATGCTGTCTATTGAGGAAATCAAAATTATTAAGAACAAGCTTGGGGTGACGATAAATGATGTTATTGCGGGAATAACCTTTCTGGGCACCCGAATGTACATGCAACGGATGAATAGTGAAAAATCAAGAGATGTTGATGGAACCAAGCAAGTCTTCTTGGGGCAACCATTTGTGTTCTTGCATGTCTCGGTGCCCAAGTCGAGTGAAGTTTCCAAGCCATTGGATTTTGTATGGGAAGCACAGAAGATAGTCAAGAGACATAGAAGCTCTTCAGCTTGGTACCTTACTATTAGGCTCTGGGATATTTTGAAGAAATTTAGAGGCTTTGAGGTAGTGGCTGGATACATTCATAGCACACTCATGAACTCAAGCATGGCAATAACAAATATGATCGGGCCACTGGAACAAATGGCTTTGGCTGATCACCCCATTAAAGGAATCTACTTTATGGTGGTCGATTCACCTCAGCCGACGAATGGGAATCTCCAATGTCTACAAATCTACAATACTGCGTTGGGCAGCATTTCAAGCATGGAAAATGCGTTCGTGTCCAGGGCCAGTTGCTTTGTTGCTGTAGCCATTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

353

Amino Acids

39.48

Weight (kDa)

9.1

Isoelectric Point (pI)

47.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 25 - 180 1.3e-08 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 215 - 314 3.1e-18 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018004)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23651
prunus_persica Prupe.3G104400_v2.0.a1 Prupe.4G218200_v2.0.a1 Prupe.4G218200_v2.0.a1
rosa_multiflora Rmu_sc0007415.1_g000035
rosa_samantha Rh1AG225100 Rh1CG209900 Rh2BG198500 Rh5AG299700 Rh7AG423800
rosa_wichuraiana Rw4G032200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 753
AccB1I GGYRCC 3 cut(s) 560, 664, 753
AccI GTMKAC 2 cut(s) 467, 967
AciI CCGC 1 cut(s) 542
AclWI GGATC 1 cut(s) 172
AcsI RAATTY 1 cut(s) 788
AcuI CTGAAG 1 cut(s) 729
AfaI GTAC 3 cut(s) 140, 572, 755
AfiI CCNNNNNNNGG 2 cut(s) 908, 950
AgsI TTSAA 7 cut(s) 40, 136, 175, 388, 404, 784, 1002
AhlI ACTAGT 1 cut(s) 299
AjnI CCWGG 1 cut(s) 1025
AjuI GAANNNNNNNTTGG 2 cut(s) 611, 643
AloI GAACNNNNNNTCC 2 cut(s) 1001, 1033
AluBI AGCT 4 cut(s) 158, 514, 741, 749
AluI AGCT 4 cut(s) 158, 514, 741, 749
Alw26I GTCTC 3 cut(s) 236, 664, 724
AlwI GGATC 1 cut(s) 172
AoxI GGCC 4 cut(s) 110, 452, 869, 1029
ApeKI GCWGC 2 cut(s) 155, 993
ApoI RAATTY 1 cut(s) 788
Asp700I GAANNNNTTC 1 cut(s) 742
Asp718I GGTACC 1 cut(s) 753
AspLEI GCGC 1 cut(s) 218
AspS9I GGNCC 3 cut(s) 426, 869, 1029
AsuHPI GGTGA 3 cut(s) 532, 890, 927
AvaII GGWCC 1 cut(s) 426
BaeGI GKGCMC 3 cut(s) 395, 563, 669
BanI GGYRCC 3 cut(s) 560, 664, 753
BauI CACGAG 1 cut(s) 312
BbsI GAAGAC 1 cut(s) 619
BbvCI CCTCAGC 1 cut(s) 939
BbvI GCAGC 2 cut(s) 167, 1005
BccI CCATC 2 cut(s) 191, 605
BceAI ACGGC 1 cut(s) 332
BcgI CGANNNNNNTGC 2 cut(s) 457, 491
BciT130I CCWGG 1 cut(s) 1027
BciVI GTATCC 1 cut(s) 809
BclI TGATCA 1 cut(s) 895
BcoDI GTCTC 3 cut(s) 236, 664, 724
BcuI ACTAGT 1 cut(s) 299
BfaI CTAG 2 cut(s) 26, 300
BfmI CTRYAG 1 cut(s) 1047
BfoI RGCGCY 1 cut(s) 219
BfuI GTATCC 1 cut(s) 809
BisI GCNGC 2 cut(s) 156, 994
BlsI GCNGC 2 cut(s) 157, 995
Bme1390I CCNGG 1 cut(s) 1027
Bme18I GGWCC 1 cut(s) 426
BmgT120I GGNCC 3 cut(s) 426, 869, 1029
BmiI GGNNCC 4 cut(s) 562, 616, 666, 755
BmrFI CCNGG 1 cut(s) 1027
BmsI GCATC 2 cut(s) 95, 465
BpiI GAAGAC 1 cut(s) 619
Bpu10I CCTNAGC 1 cut(s) 939
BpuEI CTTGAG 1 cut(s) 826
BsaJI CCNNGG 2 cut(s) 190, 1026
Bsc4I CCNNNNNNNGG 2 cut(s) 908, 950
Bse1I ACTGG 2 cut(s) 879, 1032
BseBI CCWGG 1 cut(s) 1027
BseDI CCNNGG 2 cut(s) 190, 1026
BseGI GGATG 1 cut(s) 588
BseLI CCNNNNNNNGG 2 cut(s) 908, 950
BseMII CTCAG 1 cut(s) 953
BseNI ACTGG 2 cut(s) 879, 1032
BseSI GKGCMC 3 cut(s) 395, 563, 669
BseXI GCAGC 2 cut(s) 167, 1005
BshFI GGCC 4 cut(s) 112, 454, 871, 1031
BshNI GGYRCC 3 cut(s) 560, 664, 753
BsiSI CCGG 1 cut(s) 429
BslI CCNNNNNNNGG 2 cut(s) 908, 950
BsmAI GTCTC 3 cut(s) 236, 664, 724
BsnI GGCC 4 cut(s) 112, 454, 871, 1031
Bsp1286I GDGCHC 3 cut(s) 395, 563, 669
Bsp1407I TGTACA 1 cut(s) 570
Bsp143I GATC 4 cut(s) 177, 409, 864, 895
BspACI CCGC 1 cut(s) 542
BspANI GGCC 4 cut(s) 112, 454, 871, 1031
BspCNI CTCAG 1 cut(s) 952
BspHI TCATGA 1 cut(s) 834
BspLI GGNNCC 4 cut(s) 562, 616, 666, 755
BspPI GGATC 1 cut(s) 172
BspQI GCTCTTC 1 cut(s) 748
BspT107I GGYRCC 3 cut(s) 560, 664, 753
BsrGI TGTACA 1 cut(s) 570
BsrI ACTGG 2 cut(s) 879, 1032
BssECI CCNNGG 2 cut(s) 190, 1026
BssMI GATC 4 cut(s) 177, 409, 864, 895
BssSI CACGAG 1 cut(s) 312
BssT1I CCWWGG 1 cut(s) 190
Bst2BI CACGAG 1 cut(s) 312
Bst2UI CCWGG 1 cut(s) 1027
Bst4CI ACNGT 1 cut(s) 359
Bst6I CTCTTC 2 cut(s) 261, 748
BstAUI TGTACA 1 cut(s) 570
BstC8I GCNNGC 1 cut(s) 110
BstDEI CTNAG 2 cut(s) 333, 939
BstF5I GGATG 1 cut(s) 588
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 4 cut(s) 180, 412, 867, 898
BstMAI GTCTC 3 cut(s) 236, 664, 724
BstMBI GATC 4 cut(s) 177, 409, 864, 895
BstMWI GCNNNNNNNGC 1 cut(s) 1002
BstNI CCWGG 1 cut(s) 1027
BstNSI RCATGY 2 cut(s) 577, 659
BstSCI CCNGG 1 cut(s) 1025
BstSFI CTRYAG 1 cut(s) 1047
BstSLI GKGCMC 3 cut(s) 395, 563, 669
BstV1I GCAGC 2 cut(s) 167, 1005
BstV2I GAAGAC 1 cut(s) 619
BstX2I RGATCY 1 cut(s) 177
BstXI CCANNNNNNTGG 2 cut(s) 191, 694
BstYI RGATCY 1 cut(s) 177
BsuI GTATCC 1 cut(s) 809
BsuRI GGCC 4 cut(s) 112, 454, 871, 1031
BtgZI GCGATG 1 cut(s) 210
BtsCI GGATG 1 cut(s) 588
BtsIMutI CAGTG 1 cut(s) 872
Cac8I GCNNGC 1 cut(s) 110
CciI TCATGA 1 cut(s) 834
CfoI GCGC 1 cut(s) 218
Cfr13I GGNCC 3 cut(s) 426, 869, 1029
Csp6I GTAC 3 cut(s) 139, 571, 754
CviAII CATG 9 cut(s) 152, 185, 363, 463, 574, 656, 835, 847, 1006
CviQI GTAC 3 cut(s) 139, 571, 754
DdeI CTNAG 2 cut(s) 333, 939
DpnI GATC 4 cut(s) 179, 411, 866, 897
DpnII GATC 4 cut(s) 177, 409, 864, 895
Eam1104I CTCTTC 2 cut(s) 261, 748
EarI CTCTTC 2 cut(s) 261, 748
Eco130I CCWWGG 1 cut(s) 190
Eco47I GGWCC 1 cut(s) 426
Eco57I CTGAAG 1 cut(s) 729
EcoRII CCWGG 1 cut(s) 1025
EcoT14I CCWWGG 1 cut(s) 190
ErhI CCWWGG 1 cut(s) 190
FaeI CATG 9 cut(s) 155, 188, 366, 466, 577, 659, 838, 850, 1009
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FatI CATG 9 cut(s) 151, 184, 362, 462, 573, 655, 834, 846, 1005
FauI CCCGC 1 cut(s) 535
FbaI TGATCA 1 cut(s) 895
FblI GTMKAC 2 cut(s) 467, 967
Fnu4HI GCNGC 2 cut(s) 156, 994
FokI GGATG 1 cut(s) 595
Fsp4HI GCNGC 2 cut(s) 156, 994
FspBI CTAG 2 cut(s) 26, 300
GlaI GCGC 1 cut(s) 217
GluI GCNGC 2 cut(s) 156, 994
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 4 cut(s) 112, 454, 871, 1031
HapII CCGG 1 cut(s) 429
HhaI GCGC 1 cut(s) 218
Hin1II CATG 9 cut(s) 155, 188, 366, 466, 577, 659, 838, 850, 1009
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HincII GTYRAC 1 cut(s) 468
HindII GTYRAC 1 cut(s) 468
HindIII AAGCTT 1 cut(s) 512
HinfI GANTC 4 cut(s) 40, 912, 932, 955
HpaII CCGG 1 cut(s) 429
HphI GGTGA 3 cut(s) 532, 890, 927
Hpy166II GTNNAC 2 cut(s) 468, 968
Hpy188I TCNGA 1 cut(s) 91
Hpy188III TCNNGA 5 cut(s) 26, 175, 600, 727, 835
Hpy8I GTNNAC 2 cut(s) 468, 968
Hpy99I CGWCG 1 cut(s) 949
HpyAV CCTTC 3 cut(s) 261, 282, 382
HpyCH4III ACNGT 1 cut(s) 359
HpyCH4V TGCA 5 cut(s) 155, 287, 362, 577, 655
HpyF10VI GCNNNNNNNGC 1 cut(s) 1002
HpyF3I CTNAG 2 cut(s) 333, 939
Hsp92II CATG 9 cut(s) 155, 188, 366, 466, 577, 659, 838, 850, 1009
HspAI GCGC 1 cut(s) 216
KpnI GGTACC 1 cut(s) 757
Ksp22I TGATCA 1 cut(s) 895
Kzo9I GATC 4 cut(s) 177, 409, 864, 895
LguI GCTCTTC 1 cut(s) 748
LmnI GCTCC 1 cut(s) 378
Lsp1109I GCAGC 2 cut(s) 167, 1005
LweI GCATC 2 cut(s) 95, 465
MaeI CTAG 2 cut(s) 26, 300
MaeIII GTNAC 1 cut(s) 520
MalI GATC 4 cut(s) 179, 411, 866, 897
MboI GATC 4 cut(s) 177, 409, 864, 895
MboII GAAGA 7 cut(s) 91, 248, 416, 619, 730, 735, 796
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 3 cut(s) 395, 563, 669
MluCI AATT 2 cut(s) 498, 788
MmeI TCCRAC 2 cut(s) 356, 418
MnlI CCTC 5 cut(s) 33, 481, 788, 796, 948
MroXI GAANNNNTTC 1 cut(s) 742
MseI TTAA 3 cut(s) 504, 906, 1056
MslI CAYNNNNRTG 2 cut(s) 566, 833
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 1 cut(s) 429
MspR9I CCNGG 1 cut(s) 1027
MvaI CCWGG 1 cut(s) 1027
MwoI GCNNNNNNNGC 1 cut(s) 1002
NdeII GATC 4 cut(s) 177, 409, 864, 895
NlaIII CATG 9 cut(s) 155, 188, 366, 466, 577, 659, 838, 850, 1009
NlaIV GGNNCC 4 cut(s) 562, 616, 666, 755
NmuCI GTSAC 1 cut(s) 520
NspI RCATGY 2 cut(s) 577, 659
PagI TCATGA 1 cut(s) 834
PciSI GCTCTTC 1 cut(s) 748
PdmI GAANNNNTTC 1 cut(s) 742
PfeI GAWTC 4 cut(s) 40, 912, 932, 955
PkrI GCNGC 2 cut(s) 157, 995
Psp6I CCWGG 1 cut(s) 1025
PspGI CCWGG 1 cut(s) 1025
PspN4I GGNNCC 4 cut(s) 562, 616, 666, 755
PspPI GGNCC 3 cut(s) 426, 869, 1029
PsuI RGATCY 1 cut(s) 177
PvuII CAGCTG 1 cut(s) 158
RsaI GTAC 3 cut(s) 140, 572, 755
RsaNI GTAC 3 cut(s) 139, 571, 754
RseI CAYNNNNRTG 2 cut(s) 566, 833
SalI GTCGAC 1 cut(s) 466
SapI GCTCTTC 1 cut(s) 748
SaqAI TTAA 3 cut(s) 504, 906, 1056
SatI GCNGC 2 cut(s) 156, 994
Sau3AI GATC 4 cut(s) 177, 409, 864, 895
Sau96I GGNCC 3 cut(s) 426, 869, 1029
ScrFI CCNGG 1 cut(s) 1027
SduI GDGCHC 3 cut(s) 395, 563, 669
SfaNI GCATC 2 cut(s) 95, 465
SfcI CTRYAG 1 cut(s) 1047
SinI GGWCC 1 cut(s) 426
SmiMI CAYNNNNRTG 2 cut(s) 566, 833
SmlI CTYRAG 1 cut(s) 841
SmoI CTYRAG 1 cut(s) 841
SpeI ACTAGT 1 cut(s) 299
Sse9I AATT 2 cut(s) 498, 788
SsiI CCGC 1 cut(s) 542
SspI AATATT 1 cut(s) 171
SspMI CTAG 2 cut(s) 26, 300
StyD4I CCNGG 1 cut(s) 1025
StyI CCWWGG 1 cut(s) 190
TaaI ACNGT 1 cut(s) 359
TaqI TCGA 4 cut(s) 412, 467, 674, 930
TasI AATT 2 cut(s) 498, 788
TatI WGTACW 1 cut(s) 570
TfiI GAWTC 4 cut(s) 40, 912, 932, 955
Tru1I TTAA 3 cut(s) 504, 906, 1056
Tru9I TTAA 3 cut(s) 504, 906, 1056
TscAI CASTG 1 cut(s) 879
TseFI GTSAC 1 cut(s) 520
TseI GCWGC 2 cut(s) 155, 993
Tsp45I GTSAC 1 cut(s) 520
TspDTI ATGAA 5 cut(s) 116, 309, 599, 812, 851
TspGWI ACGGA 1 cut(s) 595
TspRI CASTG 1 cut(s) 879
VpaK11BI GGWCC 1 cut(s) 426
XapI RAATTY 1 cut(s) 788
XbaI TCTAGA 1 cut(s) 25
XceI RCATGY 2 cut(s) 577, 659
XcmI CCANNNNNNNNNTGG 1 cut(s) 188
XmiI GTMKAC 2 cut(s) 467, 967
XmnI GAANNNNTTC 1 cut(s) 742
XspI CTAG 2 cut(s) 26, 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.