Rh1CG209900

O-acyltransferase (WSD1-like)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0018004
Physical Location & Seq
Forward (+)
45262591 .. 45305205
42615 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG209900.1

Sequence Viewer

Length: 1473 bp
ATGAATTCTGAAAATCCCACAATTCCACCAAACAGTACCCAAGACACTACTAATAACCCCAACGCCATTCCTATTCCGAGTGCGGAGCCTAATCCAACTGAACAAGGAGGAGCTGTACCTGCTCCACCTCCTCCTGTACCTTGTGTTAGAAAGAGGAAACCTGCTAGGAACGCCTCTGATGTGTGGAATCATTTCAAAAAGGTAGATGAGAATGGTGAGGATCCTAGATGCATTTGTAAGCATTGTGGGACAGACTATGCTTGTGATCCAAAGAGGAATGGCACTTCCACTTTGTGGAATCATGTGAGGAACCAATGCAAACGTTCTCCACTGAGAATTGATAAGAAACAAAAGCTCCTAGCTTTTGAAAAAAAGGATGGAGAAGCTGAACAAAATGGAGCTCCTCAAGGTGGATCTCAAGACAAAACTCAAGATGGAACTCAAGGTGAAACTCAAGTTGAAGGTGCGGATGAAAAGAATAAGCAGTGGAAGAGGGTTCAAGTGAAGCTGGAAGACCATGTGCCATTGTGGGAACTTCATCTTTTCAAGTACCCAACAAGTCATGCAGCTGGACAAATAATATTCAAGATCCACCATGCCCTTGGCGATGGCTACTCTCTTATGGGCGCTTATCTCTCTTGTCTCCAAAATGCTCACAATCCTTCTCTTCCCCTGACATTTCCTTCACTAAAGGGTGCAAAGAATGAAACTAGTAGTCTATCTCGTGTGTTTGAGTTTGTGCCTAAGATACTTTCTGCCGTTATCAACGGTGCATGGGATTTCAGTTGGAGCATTTTGAAGGGCACTTGGGTTGAAGATGATCGAACACCAACAAGGTCCGGCGTTGTTGGAGTTGAGTTTCGGCCTGTGTCCATGTCGACCTTGATGCTGTCTATTGAGGAAATCAAAATTATTAAGAACAAGCTTGGGGTGACGATAAATGATGTTATTGCGGGAATAACCTTTCTGGGCACCCGAATGTACATGCAACGGATGAATAGTGAAAAATCAAGAGATGTTGATGGAACCAAGCAAGTCTTCTTGGGGCAACCATTTGTGTTCTTGCATGTCTCGGTGCCCAAGTCGAGTGAAGTTTCCAAGCCATTGGATTTTGTATGGGAAGCACAGAAGATAGTCAAGAGACATAGAAGCTCTTCAGCTTGGTACCTTACTATTAGGCTCTGGGATATTTTGAAGAAATTTAGAGGCTTTGAGGTAGTGGCTGGATACATTCATAGCACACTCATGAACTCAAGCATGGCAATAACAAATATGATCGGGCCACTGGAACAAATGGCTTTGGCTGATCACCCCATTAAAGGAATCTACTTTATGGTGGTCGATTCACCTCAGCCGACGAATGGGAATCTCCAATGTCTACAAATCTACAATACTGCGTTGGGCAGCATTTCAAGCATGGAAAATGCGTTCGTGTCCAGGGCCAGTTGCTTTGTTGCTGTAGCCATTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1298 WES_acyltransf

Protein Analysis

490

Amino Acids

54.49

Weight (kDa)

8.96

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-BED PF02892 59 - 107 1.1e-06 BED zinc finger
WS_DGAT_cat PF03007 169 - 317 1.2e-06 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 352 - 451 5.5e-18 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018004)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23651
prunus_persica Prupe.3G104400_v2.0.a1 Prupe.4G218200_v2.0.a1 Prupe.4G218200_v2.0.a1
rosa_multiflora Rmu_sc0007415.1_g000035
rosa_samantha Rh1AG225100 Rh1CG209900 Rh2BG198500 Rh5AG299700 Rh7AG423800
rosa_wichuraiana Rw4G032200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 127, 169
Acc65I GGTACC 1 cut(s) 1164
AccB1I GGYRCC 3 cut(s) 971, 1075, 1164
AccB7I CCANNNNNTGG 1 cut(s) 294
AccI GTMKAC 2 cut(s) 878, 1378
AciI CCGC 3 cut(s) 83, 467, 953
AclI AACGTT 1 cut(s) 322
AclWI GGATC 5 cut(s) 215, 228, 260, 421, 583
AcsI RAATTY 2 cut(s) 4, 1199
AcuI CTGAAG 1 cut(s) 1140
AdeI CACNNNGTG 1 cut(s) 294
AfaI GTAC 6 cut(s) 37, 117, 138, 551, 983, 1166
AfiI CCNNNNNNNGG 4 cut(s) 294, 410, 1319, 1361
AhlI ACTAGT 1 cut(s) 710
AjnI CCWGG 1 cut(s) 1436
AjuI GAANNNNNNNTTGG 2 cut(s) 1022, 1054
AloI GAACNNNNNNTCC 2 cut(s) 1412, 1444
Alw21I GWGCWC 1 cut(s) 403
Alw26I GTCTC 3 cut(s) 647, 1075, 1135
AlwI GGATC 5 cut(s) 215, 228, 260, 421, 583
AoxI GGCC 3 cut(s) 863, 1280, 1440
ApeKI GCWGC 2 cut(s) 566, 1404
ApoI RAATTY 2 cut(s) 4, 1199
Asp700I GAANNNNTTC 2 cut(s) 191, 1153
Asp718I GGTACC 1 cut(s) 1164
AspLEI GCGC 1 cut(s) 629
AspS9I GGNCC 3 cut(s) 837, 1280, 1440
AsuHPI GGTGA 5 cut(s) 227, 458, 943, 1301, 1338
AvaII GGWCC 1 cut(s) 837
BaeGI GKGCMC 3 cut(s) 806, 974, 1080
BamHI GGATCC 1 cut(s) 220
BanI GGYRCC 3 cut(s) 971, 1075, 1164
BanII GRGCYC 1 cut(s) 403
BauI CACGAG 1 cut(s) 723
BbsI GAAGAC 2 cut(s) 519, 1030
Bbv12I GWGCWC 1 cut(s) 403
BbvCI CCTCAGC 1 cut(s) 1350
BbvI GCAGC 2 cut(s) 578, 1416
BccI CCATC 4 cut(s) 371, 428, 602, 1016
BceAI ACGGC 1 cut(s) 743
BcgI CGANNNNNNTGC 2 cut(s) 868, 902
BciT130I CCWGG 1 cut(s) 1438
BciVI GTATCC 1 cut(s) 1220
BclI TGATCA 1 cut(s) 1306
BcoDI GTCTC 3 cut(s) 647, 1075, 1135
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 4 cut(s) 165, 225, 359, 711
BfmI CTRYAG 1 cut(s) 1458
BfoI RGCGCY 1 cut(s) 630
BfuAI ACCTGC 2 cut(s) 127, 169
BfuI GTATCC 1 cut(s) 1220
BisI GCNGC 2 cut(s) 567, 1405
BlsI GCNGC 2 cut(s) 568, 1406
Bme1390I CCNGG 1 cut(s) 1438
Bme18I GGWCC 1 cut(s) 837
BmgT120I GGNCC 3 cut(s) 837, 1280, 1440
BmiI GGNNCC 7 cut(s) 87, 222, 311, 973, 1027, 1077, 1166
BmrFI CCNGG 1 cut(s) 1438
BmsI GCATC 2 cut(s) 218, 876
BpiI GAAGAC 2 cut(s) 519, 1030
Bpu10I CCTNAGC 1 cut(s) 1350
BpuEI CTTGAG 6 cut(s) 390, 402, 414, 426, 438, 1237
BsaJI CCNNGG 2 cut(s) 601, 1437
BsaXI ACNNNNNCTCC 4 cut(s) 99, 129, 339, 369
Bsc4I CCNNNNNNNGG 4 cut(s) 294, 410, 1319, 1361
Bse1I ACTGG 2 cut(s) 1290, 1443
BseBI CCWGG 1 cut(s) 1438
BseDI CCNNGG 2 cut(s) 601, 1437
BseGI GGATG 3 cut(s) 382, 475, 999
BseLI CCNNNNNNNGG 4 cut(s) 294, 410, 1319, 1361
BseMII CTCAG 2 cut(s) 323, 1364
BseNI ACTGG 2 cut(s) 1290, 1443
BseRI GAGGAG 3 cut(s) 120, 123, 393
BseSI GKGCMC 3 cut(s) 806, 974, 1080
BseXI GCAGC 2 cut(s) 578, 1416
BshFI GGCC 3 cut(s) 865, 1282, 1442
BshNI GGYRCC 3 cut(s) 971, 1075, 1164
BsiHKAI GWGCWC 1 cut(s) 403
BsiSI CCGG 1 cut(s) 840
BslFI GGGAC 1 cut(s) 262
BslI CCNNNNNNNGG 4 cut(s) 294, 410, 1319, 1361
BsmAI GTCTC 3 cut(s) 647, 1075, 1135
BsmFI GGGAC 1 cut(s) 262
BsnI GGCC 3 cut(s) 865, 1282, 1442
Bsp1286I GDGCHC 4 cut(s) 403, 806, 974, 1080
Bsp1407I TGTACA 1 cut(s) 981
Bsp143I GATC 7 cut(s) 220, 265, 413, 588, 820, 1275, 1306
BspACI CCGC 3 cut(s) 83, 467, 953
BspANI GGCC 3 cut(s) 865, 1282, 1442
BspCNI CTCAG 2 cut(s) 324, 1363
BspHI TCATGA 1 cut(s) 1245
BspLI GGNNCC 7 cut(s) 87, 222, 311, 973, 1027, 1077, 1166
BspMI ACCTGC 2 cut(s) 127, 169
BspPI GGATC 5 cut(s) 215, 228, 260, 421, 583
BspQI GCTCTTC 1 cut(s) 1159
BspT107I GGYRCC 3 cut(s) 971, 1075, 1164
BsrGI TGTACA 1 cut(s) 981
BsrI ACTGG 2 cut(s) 1290, 1443
BssECI CCNNGG 2 cut(s) 601, 1437
BssMI GATC 7 cut(s) 220, 265, 413, 588, 820, 1275, 1306
BssSI CACGAG 1 cut(s) 723
BssT1I CCWWGG 1 cut(s) 601
Bst2BI CACGAG 1 cut(s) 723
Bst2UI CCWGG 1 cut(s) 1438
Bst4CI ACNGT 2 cut(s) 35, 770
Bst6I CTCTTC 3 cut(s) 485, 672, 1159
BstAUI TGTACA 1 cut(s) 981
BstDEI CTNAG 3 cut(s) 332, 744, 1350
BstF5I GGATG 3 cut(s) 382, 475, 999
BstH2I RGCGCY 1 cut(s) 630
BstHHI GCGC 1 cut(s) 629
BstKTI GATC 7 cut(s) 223, 268, 416, 591, 823, 1278, 1309
BstMAI GTCTC 3 cut(s) 647, 1075, 1135
BstMBI GATC 7 cut(s) 220, 265, 413, 588, 820, 1275, 1306
BstMWI GCNNNNNNNGC 3 cut(s) 119, 170, 1413
BstNI CCWGG 1 cut(s) 1438
BstNSI RCATGY 2 cut(s) 988, 1070
BstSCI CCNGG 1 cut(s) 1436
BstSFI CTRYAG 1 cut(s) 1458
BstSLI GKGCMC 3 cut(s) 806, 974, 1080
BstV1I GCAGC 2 cut(s) 578, 1416
BstV2I GAAGAC 2 cut(s) 519, 1030
BstX2I RGATCY 3 cut(s) 220, 413, 588
BstXI CCANNNNNNTGG 2 cut(s) 602, 1105
BstYI RGATCY 3 cut(s) 220, 413, 588
BsuI GTATCC 1 cut(s) 1220
BsuRI GGCC 3 cut(s) 865, 1282, 1442
BtgZI GCGATG 1 cut(s) 621
BtsCI GGATG 3 cut(s) 382, 475, 999
BtsI GCAGTG 1 cut(s) 491
BtsIMutI CAGTG 3 cut(s) 329, 491, 1283
BveI ACCTGC 2 cut(s) 127, 169
CciI TCATGA 1 cut(s) 1245
CfoI GCGC 1 cut(s) 629
Cfr13I GGNCC 3 cut(s) 837, 1280, 1440
Csp6I GTAC 6 cut(s) 36, 116, 137, 550, 982, 1165
CviQI GTAC 6 cut(s) 36, 116, 137, 550, 982, 1165
DdeI CTNAG 3 cut(s) 332, 744, 1350
DpnI GATC 7 cut(s) 222, 267, 415, 590, 822, 1277, 1308
DpnII GATC 7 cut(s) 220, 265, 413, 588, 820, 1275, 1306
DraIII CACNNNGTG 1 cut(s) 294
Eam1104I CTCTTC 3 cut(s) 485, 672, 1159
EarI CTCTTC 3 cut(s) 485, 672, 1159
Ecl136II GAGCTC 1 cut(s) 401
Eco130I CCWWGG 1 cut(s) 601
Eco24I GRGCYC 1 cut(s) 403
Eco47I GGWCC 1 cut(s) 837
Eco53kI GAGCTC 1 cut(s) 401
Eco57I CTGAAG 1 cut(s) 1140
EcoICRI GAGCTC 1 cut(s) 401
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 1436
EcoT14I CCWWGG 1 cut(s) 601
EcoT22I ATGCAT 1 cut(s) 233
EcoT38I GRGCYC 1 cut(s) 403
ErhI CCWWGG 1 cut(s) 601
FalI AAGNNNNNCTT 2 cut(s) 1022, 1054
FaqI GGGAC 1 cut(s) 262
FauI CCCGC 1 cut(s) 946
FbaI TGATCA 1 cut(s) 1306
FblI GTMKAC 2 cut(s) 878, 1378
Fnu4HI GCNGC 2 cut(s) 567, 1405
FokI GGATG 3 cut(s) 389, 482, 1006
FriOI GRGCYC 1 cut(s) 403
Fsp4HI GCNGC 2 cut(s) 567, 1405
FspBI CTAG 4 cut(s) 165, 225, 359, 711
GlaI GCGC 1 cut(s) 628
GluI GCNGC 2 cut(s) 567, 1405
HaeII RGCGCY 1 cut(s) 630
HaeIII GGCC 3 cut(s) 865, 1282, 1442
HapII CCGG 1 cut(s) 840
HhaI GCGC 1 cut(s) 629
Hin6I GCGC 1 cut(s) 627
HinP1I GCGC 1 cut(s) 627
HincII GTYRAC 1 cut(s) 879
HindII GTYRAC 1 cut(s) 879
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 5 cut(s) 187, 298, 1323, 1343, 1366
HpaII CCGG 1 cut(s) 840
HphI GGTGA 5 cut(s) 227, 458, 943, 1301, 1338
Hpy166II GTNNAC 2 cut(s) 879, 1379
Hpy188I TCNGA 3 cut(s) 10, 78, 178
Hpy188III TCNNGA 6 cut(s) 419, 431, 586, 1011, 1138, 1246
Hpy8I GTNNAC 2 cut(s) 879, 1379
Hpy99I CGWCG 1 cut(s) 1360
HpyAV CCTTC 4 cut(s) 455, 672, 693, 793
HpyCH4III ACNGT 2 cut(s) 35, 770
HpyCH4IV ACGT 1 cut(s) 322
HpyCH4V TGCA 7 cut(s) 231, 318, 566, 698, 773, 988, 1066
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 170, 1413
HpyF3I CTNAG 3 cut(s) 332, 744, 1350
HpySE526I ACGT 1 cut(s) 322
HspAI GCGC 1 cut(s) 627
KpnI GGTACC 1 cut(s) 1168
Ksp22I TGATCA 1 cut(s) 1306
Kzo9I GATC 7 cut(s) 220, 265, 413, 588, 820, 1275, 1306
LguI GCTCTTC 1 cut(s) 1159
LmnI GCTCC 7 cut(s) 85, 110, 127, 360, 398, 406, 789
Lsp1109I GCAGC 2 cut(s) 578, 1416
LweI GCATC 2 cut(s) 218, 876
MaeI CTAG 4 cut(s) 165, 225, 359, 711
MaeII ACGT 1 cut(s) 322
MaeIII GTNAC 1 cut(s) 931
MalI GATC 7 cut(s) 222, 267, 415, 590, 822, 1277, 1308
MboI GATC 7 cut(s) 220, 265, 413, 588, 820, 1275, 1306
MboII GAAGA 8 cut(s) 502, 524, 659, 827, 1030, 1141, 1146, 1207
MflI RGATCY 3 cut(s) 220, 413, 588
MhlI GDGCHC 4 cut(s) 403, 806, 974, 1080
MluCI AATT 5 cut(s) 4, 21, 336, 909, 1199
MmeI TCCRAC 3 cut(s) 119, 767, 829
Mph1103I ATGCAT 1 cut(s) 233
MroXI GAANNNNTTC 2 cut(s) 191, 1153
MseI TTAA 3 cut(s) 915, 1317, 1467
MslI CAYNNNNRTG 2 cut(s) 977, 1244
MspA1I CMGCKG 1 cut(s) 569
MspI CCGG 1 cut(s) 840
MspR9I CCNGG 1 cut(s) 1438
MvaI CCWGG 1 cut(s) 1438
MwoI GCNNNNNNNGC 3 cut(s) 119, 170, 1413
NdeII GATC 7 cut(s) 220, 265, 413, 588, 820, 1275, 1306
NlaIV GGNNCC 7 cut(s) 87, 222, 311, 973, 1027, 1077, 1166
NmuCI GTSAC 1 cut(s) 931
NsiI ATGCAT 1 cut(s) 233
NspI RCATGY 2 cut(s) 988, 1070
PagI TCATGA 1 cut(s) 1245
PciSI GCTCTTC 1 cut(s) 1159
PdmI GAANNNNTTC 2 cut(s) 191, 1153
PfeI GAWTC 5 cut(s) 187, 298, 1323, 1343, 1366
PflMI CCANNNNNTGG 1 cut(s) 294
PkrI GCNGC 2 cut(s) 568, 1406
Psp124BI GAGCTC 1 cut(s) 403
Psp1406I AACGTT 1 cut(s) 322
Psp6I CCWGG 1 cut(s) 1436
PspGI CCWGG 1 cut(s) 1436
PspN4I GGNNCC 7 cut(s) 87, 222, 311, 973, 1027, 1077, 1166
PspPI GGNCC 3 cut(s) 837, 1280, 1440
PsuI RGATCY 3 cut(s) 220, 413, 588
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 6 cut(s) 37, 117, 138, 551, 983, 1166
RsaNI GTAC 6 cut(s) 36, 116, 137, 550, 982, 1165
RseI CAYNNNNRTG 2 cut(s) 977, 1244
SacI GAGCTC 1 cut(s) 403
SalI GTCGAC 1 cut(s) 877
SapI GCTCTTC 1 cut(s) 1159
SaqAI TTAA 3 cut(s) 915, 1317, 1467
SatI GCNGC 2 cut(s) 567, 1405
Sau3AI GATC 7 cut(s) 220, 265, 413, 588, 820, 1275, 1306
Sau96I GGNCC 3 cut(s) 837, 1280, 1440
ScrFI CCNGG 1 cut(s) 1438
SduI GDGCHC 4 cut(s) 403, 806, 974, 1080
SfaNI GCATC 2 cut(s) 218, 876
SfcI CTRYAG 1 cut(s) 1458
SinI GGWCC 1 cut(s) 837
SmiMI CAYNNNNRTG 2 cut(s) 977, 1244
SmlI CTYRAG 6 cut(s) 405, 417, 429, 441, 453, 1252
SmoI CTYRAG 6 cut(s) 405, 417, 429, 441, 453, 1252
SpeI ACTAGT 1 cut(s) 710
Sse9I AATT 5 cut(s) 4, 21, 336, 909, 1199
SsiI CCGC 3 cut(s) 83, 467, 953
SspI AATATT 1 cut(s) 582
SspMI CTAG 4 cut(s) 165, 225, 359, 711
SstI GAGCTC 1 cut(s) 403
StyD4I CCNGG 1 cut(s) 1436
StyI CCWWGG 1 cut(s) 601
TaaI ACNGT 2 cut(s) 35, 770
TaiI ACGT 1 cut(s) 325
TaqI TCGA 4 cut(s) 823, 878, 1085, 1341
TasI AATT 5 cut(s) 4, 21, 336, 909, 1199
TatI WGTACW 1 cut(s) 981
TfiI GAWTC 5 cut(s) 187, 298, 1323, 1343, 1366
Tru1I TTAA 3 cut(s) 915, 1317, 1467
Tru9I TTAA 3 cut(s) 915, 1317, 1467
TscAI CASTG 3 cut(s) 336, 491, 1290
TseFI GTSAC 1 cut(s) 931
TseI GCWGC 2 cut(s) 566, 1404
Tsp45I GTSAC 1 cut(s) 931
TspDTI ATGAA 7 cut(s) 17, 486, 527, 720, 1010, 1223, 1262
TspGWI ACGGA 1 cut(s) 1006
TspRI CASTG 3 cut(s) 336, 491, 1290
Van91I CCANNNNNTGG 1 cut(s) 294
VpaK11BI GGWCC 1 cut(s) 837
XapI RAATTY 2 cut(s) 4, 1199
XceI RCATGY 2 cut(s) 988, 1070
XcmI CCANNNNNNNNNTGG 1 cut(s) 599
XmiI GTMKAC 2 cut(s) 878, 1378
XmnI GAANNNNTTC 2 cut(s) 191, 1153
XspI CTAG 4 cut(s) 165, 225, 359, 711
Zsp2I ATGCAT 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.