Rh1BG209900

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0010621
Physical Location & Seq
Reverse (-)
32825006 .. 32826830
1825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG209900.1

Sequence Viewer

Length: 288 bp
ATGAAGCGCATCCAGAAGGGCCCCGTCCGCGGCATCTCCCTCAAGCTCCAGGAGGAGGAGCGCGAGCGCCGCATGGACTTTGTCCCCGACGAGTCCGCCATGAAGACCGACGAGATCGCCGTCGACAAGGAGACCCTCGACATGCTCACTGCTCTCGGCATGGCGGATATTCCCGGAGTCAAGAAGGTGGATCCCTTTGATCTCCAGACTGCGCCGATCGGGTACGGCCCCAGGAGATCCCAGTTTGTTTACTGTGAAGATTCATCACGGCGGCAGGTTTTACAGTAA

Protein Analysis

95

Amino Acids

10.86

Weight (kDa)

5.47

Isoelectric Point (pI)

64.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S17e PF00833 1 - 64 4.6e-22 Ribosomal S17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 265
AccI GTMKAC 1 cut(s) 123
AccII CGCG 2 cut(s) 30, 63
AciI CCGC 6 cut(s) 28, 30, 70, 96, 164, 271
AclWI GGATC 3 cut(s) 185, 198, 231
AfaI GTAC 1 cut(s) 224
AfiI CCNNNNNNNGG 2 cut(s) 29, 55
AjnI CCWGG 2 cut(s) 48, 230
AluBI AGCT 1 cut(s) 46
AluI AGCT 1 cut(s) 46
Alw26I GTCTC 1 cut(s) 125
AlwI GGATC 3 cut(s) 185, 198, 231
AoxI GGCC 2 cut(s) 19, 226
ApaI GGGCCC 1 cut(s) 23
AspLEI GCGC 4 cut(s) 9, 63, 69, 214
AspS9I GGNCC 3 cut(s) 19, 20, 227
AsuC2I CCSGG 1 cut(s) 174
BaeGI GKGCMC 1 cut(s) 23
BamHI GGATCC 1 cut(s) 190
BanII GRGCYC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 110
BceAI ACGGC 3 cut(s) 104, 241, 284
BciT130I CCWGG 2 cut(s) 50, 232
BcnI CCSGG 1 cut(s) 174
BcoDI GTCTC 1 cut(s) 125
BfoI RGCGCY 1 cut(s) 70
BfuAI ACCTGC 1 cut(s) 265
BisI GCNGC 3 cut(s) 31, 70, 272
BlsI GCNGC 3 cut(s) 32, 71, 273
Bme1390I CCNGG 3 cut(s) 50, 174, 232
BmgT120I GGNCC 3 cut(s) 19, 20, 227
BmiI GGNNCC 4 cut(s) 21, 22, 192, 229
BmrFI CCNGG 3 cut(s) 50, 174, 232
BmrI ACTGGG 1 cut(s) 235
BmsI GCATC 2 cut(s) 18, 42
BmuI ACTGGG 1 cut(s) 235
BpiI GAAGAC 1 cut(s) 110
BpmI CTGGAG 2 cut(s) 32, 188
BpuEI CTTGAG 1 cut(s) 26
BpuMI CCSGG 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 2 cut(s) 28, 230
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 55
Bse1I ACTGG 1 cut(s) 241
BseBI CCWGG 2 cut(s) 50, 232
BseDI CCNNGG 2 cut(s) 28, 230
BseGI GGATG 1 cut(s) 9
BseLI CCNNNNNNNGG 2 cut(s) 29, 55
BseNI ACTGG 1 cut(s) 241
BseRI GAGGAG 2 cut(s) 68, 71
BseSI GKGCMC 1 cut(s) 23
Bsh1236I CGCG 2 cut(s) 30, 63
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 2 cut(s) 21, 228
BsiEI CGRYCG 1 cut(s) 219
BsiSI CCGG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 2 cut(s) 29, 55
BsmAI GTCTC 1 cut(s) 125
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 2 cut(s) 21, 228
Bso31I GGTCTC 1 cut(s) 125
Bsp120I GGGCCC 1 cut(s) 19
Bsp1286I GDGCHC 1 cut(s) 23
Bsp143I GATC 5 cut(s) 114, 190, 199, 216, 236
BspACI CCGC 6 cut(s) 28, 30, 70, 96, 164, 271
BspANI GGCC 2 cut(s) 21, 228
BspFNI CGCG 2 cut(s) 30, 63
BspLI GGNNCC 4 cut(s) 21, 22, 192, 229
BspMI ACCTGC 1 cut(s) 265
BspPI GGATC 3 cut(s) 185, 198, 231
BspTNI GGTCTC 1 cut(s) 125
BsrI ACTGG 1 cut(s) 241
BssECI CCNNGG 2 cut(s) 28, 230
BssMI GATC 5 cut(s) 114, 190, 199, 216, 236
Bst2UI CCWGG 2 cut(s) 50, 232
Bst4CI ACNGT 2 cut(s) 254, 285
BstC8I GCNNGC 1 cut(s) 65
BstDSI CCRYGG 1 cut(s) 28
BstF5I GGATG 1 cut(s) 9
BstFNI CGCG 2 cut(s) 30, 63
BstH2I RGCGCY 1 cut(s) 70
BstHHI GCGC 4 cut(s) 9, 63, 69, 214
BstKTI GATC 5 cut(s) 117, 193, 202, 219, 239
BstMAI GTCTC 1 cut(s) 125
BstMBI GATC 5 cut(s) 114, 190, 199, 216, 236
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 2 cut(s) 27, 69
BstNI CCWGG 2 cut(s) 50, 232
BstNSI RCATGY 1 cut(s) 145
BstSCI CCNGG 3 cut(s) 48, 172, 230
BstSLI GKGCMC 1 cut(s) 23
BstUI CGCG 2 cut(s) 30, 63
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 2 cut(s) 190, 236
BstYI RGATCY 2 cut(s) 190, 236
BsuRI GGCC 2 cut(s) 21, 228
BtgI CCRYGG 1 cut(s) 28
BtsCI GGATG 1 cut(s) 9
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 147
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 1 cut(s) 65
CfoI GCGC 4 cut(s) 9, 63, 69, 214
Cfr13I GGNCC 3 cut(s) 19, 20, 227
Cfr42I CCGCGG 1 cut(s) 31
Csp6I GTAC 1 cut(s) 223
CviAII CATG 4 cut(s) 73, 100, 142, 160
CviJI RGCY 3 cut(s) 21, 46, 228
CviKI_1 RGCY 3 cut(s) 21, 46, 228
CviQI GTAC 1 cut(s) 223
DpnI GATC 5 cut(s) 116, 192, 201, 218, 238
DpnII GATC 5 cut(s) 114, 190, 199, 216, 236
EciI GGCGGA 2 cut(s) 85, 179
Eco24I GRGCYC 1 cut(s) 23
Eco31I GGTCTC 1 cut(s) 125
EcoO109I RGGNCCY 2 cut(s) 19, 20
EcoRII CCWGG 2 cut(s) 48, 230
EcoT38I GRGCYC 1 cut(s) 23
FaeI CATG 4 cut(s) 76, 103, 145, 163
FaiI YATR 4 cut(s) 74, 101, 143, 161
FaqI GGGAC 1 cut(s) 68
FatI CATG 4 cut(s) 72, 99, 141, 159
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 3 cut(s) 31, 70, 272
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 3 cut(s) 31, 70, 272
GlaI GCGC 4 cut(s) 8, 62, 68, 213
GluI GCNGC 3 cut(s) 31, 70, 272
GsuI CTGGAG 2 cut(s) 32, 188
HaeII RGCGCY 1 cut(s) 70
HaeIII GGCC 2 cut(s) 21, 228
HapII CCGG 1 cut(s) 174
HhaI GCGC 4 cut(s) 9, 63, 69, 214
Hin1II CATG 4 cut(s) 76, 103, 145, 163
Hin6I GCGC 4 cut(s) 7, 61, 67, 212
HinP1I GCGC 4 cut(s) 7, 61, 67, 212
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 3 cut(s) 92, 177, 260
HpaII CCGG 1 cut(s) 174
Hpy166II GTNNAC 2 cut(s) 124, 250
Hpy188III TCNNGA 3 cut(s) 13, 181, 205
Hpy8I GTNNAC 2 cut(s) 124, 250
Hpy99I CGWCG 3 cut(s) 92, 113, 125
HpyAV CCTTC 2 cut(s) 10, 178
HpyCH4III ACNGT 2 cut(s) 254, 285
HpyF10VI GCNNNNNNNGC 2 cut(s) 27, 69
Hsp92II CATG 4 cut(s) 76, 103, 145, 163
HspAI GCGC 4 cut(s) 7, 61, 67, 212
KspI CCGCGG 1 cut(s) 31
Kzo9I GATC 5 cut(s) 114, 190, 199, 216, 236
LmnI GCTCC 2 cut(s) 51, 58
LpnPI CCDG 9 cut(s) 26, 35, 62, 187, 217, 218, 244, 254, 260
LweI GCATC 2 cut(s) 18, 42
MalI GATC 5 cut(s) 116, 192, 201, 218, 238
MboI GATC 5 cut(s) 114, 190, 199, 216, 236
MboII GAAGA 2 cut(s) 115, 269
MflI RGATCY 2 cut(s) 190, 236
MhlI GDGCHC 1 cut(s) 23
MlyI GAGTC 2 cut(s) 101, 186
MnlI CCTC 4 cut(s) 46, 49, 50, 146
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 3 cut(s) 50, 174, 232
MvaI CCWGG 2 cut(s) 50, 232
MvnI CGCG 2 cut(s) 30, 63
MwoI GCNNNNNNNGC 2 cut(s) 27, 69
NciI CCSGG 1 cut(s) 174
NdeII GATC 5 cut(s) 114, 190, 199, 216, 236
NlaIII CATG 4 cut(s) 76, 103, 145, 163
NlaIV GGNNCC 4 cut(s) 21, 22, 192, 229
NmeAIII GCCGAG 1 cut(s) 135
NspI RCATGY 1 cut(s) 145
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PfeI GAWTC 1 cut(s) 260
PflFI GACNNNGTC 1 cut(s) 80
PfoI TCCNGGA 2 cut(s) 48, 172
PkrI GCNGC 3 cut(s) 32, 71, 273
Ple19I CGATCG 1 cut(s) 219
PleI GAGTC 2 cut(s) 100, 185
PpsI GAGTC 2 cut(s) 100, 185
Psp6I CCWGG 2 cut(s) 48, 230
PspGI CCWGG 2 cut(s) 48, 230
PspN4I GGNNCC 4 cut(s) 21, 22, 192, 229
PspOMI GGGCCC 1 cut(s) 19
PspPI GGNCC 3 cut(s) 19, 20, 227
PsuI RGATCY 2 cut(s) 190, 236
PsyI GACNNNGTC 1 cut(s) 80
PvuI CGATCG 1 cut(s) 219
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SacII CCGCGG 1 cut(s) 31
SalI GTCGAC 1 cut(s) 122
SatI GCNGC 3 cut(s) 31, 70, 272
Sau3AI GATC 5 cut(s) 114, 190, 199, 216, 236
Sau96I GGNCC 3 cut(s) 19, 20, 227
SchI GAGTC 2 cut(s) 101, 186
ScrFI CCNGG 3 cut(s) 50, 174, 232
SduI GDGCHC 1 cut(s) 23
SetI ASST 3 cut(s) 48, 189, 279
SfaNI GCATC 2 cut(s) 18, 42
Sfr303I CCGCGG 1 cut(s) 31
SgrBI CCGCGG 1 cut(s) 31
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
SsiI CCGC 6 cut(s) 28, 30, 70, 96, 164, 271
StyD4I CCNGG 3 cut(s) 48, 172, 230
TaaI ACNGT 2 cut(s) 254, 285
TaqI TCGA 2 cut(s) 123, 138
TaqII GACCGA 1 cut(s) 122
TauI GCSGC 3 cut(s) 33, 72, 274
TfiI GAWTC 1 cut(s) 260
TscAI CASTG 1 cut(s) 154
TspDTI ATGAA 3 cut(s) 17, 116, 252
TspRI CASTG 1 cut(s) 154
Tth111I GACNNNGTC 1 cut(s) 80
XceI RCATGY 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.