Rh5AG132300

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010621
Physical Location & Seq
Forward (+)
13486030 .. 13486364
335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG132300.1

Sequence Viewer

Length: 243 bp
ATGAAGCGCATCCAGAAGGGCCCCGTCCGCGTCATCTCCCTCAAGCTCCAGGAGGAGGAGCGCAAGCGCCGCATGGACTTTGTCCCCGACGAGTCCGCCATGAAGACCGACGAGATCGCCGTCGACAAGGTGACCCTCGACATGCTCACTGCCCTCGGCATGGCTGATATTCCCGGAGTCAAGAAGGTGGATCCCTCTGATCTCCAGACTGCGCCGATCGGGTACGGCCCCAGGAGGTACTAG

Protein Analysis

80

Amino Acids

9.04

Weight (kDa)

9.05

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S17e PF00833 1 - 64 4.1e-20 Ribosomal S17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 123
AccII CGCG 1 cut(s) 30
AciI CCGC 3 cut(s) 28, 70, 96
AclWI GGATC 2 cut(s) 185, 198
AfaI GTAC 2 cut(s) 224, 239
AfiI CCNNNNNNNGG 2 cut(s) 55, 160
AjnI CCWGG 2 cut(s) 48, 230
AluBI AGCT 1 cut(s) 46
AluI AGCT 1 cut(s) 46
AlwI GGATC 2 cut(s) 185, 198
AoxI GGCC 2 cut(s) 19, 226
ApaI GGGCCC 1 cut(s) 23
AspLEI GCGC 4 cut(s) 9, 63, 69, 214
AspS9I GGNCC 3 cut(s) 19, 20, 227
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 1 cut(s) 142
BaeGI GKGCMC 1 cut(s) 23
BamHI GGATCC 1 cut(s) 190
BanII GRGCYC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 110
BceAI ACGGC 2 cut(s) 104, 241
BciT130I CCWGG 2 cut(s) 50, 232
BcnI CCSGG 1 cut(s) 174
BfaI CTAG 1 cut(s) 241
BfoI RGCGCY 1 cut(s) 70
BisI GCNGC 1 cut(s) 70
BlsI GCNGC 1 cut(s) 71
Bme1390I CCNGG 3 cut(s) 50, 174, 232
BmgT120I GGNCC 3 cut(s) 19, 20, 227
BmiI GGNNCC 4 cut(s) 21, 22, 192, 229
BmrFI CCNGG 3 cut(s) 50, 174, 232
BmsI GCATC 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 110
BpmI CTGGAG 2 cut(s) 32, 188
BpuEI CTTGAG 1 cut(s) 26
BpuMI CCSGG 1 cut(s) 174
BsaJI CCNNGG 2 cut(s) 154, 230
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 160
BseBI CCWGG 2 cut(s) 50, 232
BseDI CCNNGG 2 cut(s) 154, 230
BseGI GGATG 1 cut(s) 9
BseLI CCNNNNNNNGG 2 cut(s) 55, 160
BseRI GAGGAG 2 cut(s) 68, 71
BseSI GKGCMC 1 cut(s) 23
Bsh1236I CGCG 1 cut(s) 30
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 2 cut(s) 21, 228
BsiEI CGRYCG 1 cut(s) 219
BsiSI CCGG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 2 cut(s) 55, 160
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 2 cut(s) 21, 228
Bsp120I GGGCCC 1 cut(s) 19
Bsp1286I GDGCHC 1 cut(s) 23
Bsp143I GATC 4 cut(s) 114, 190, 199, 216
BspACI CCGC 3 cut(s) 28, 70, 96
BspANI GGCC 2 cut(s) 21, 228
BspFNI CGCG 1 cut(s) 30
BspLI GGNNCC 4 cut(s) 21, 22, 192, 229
BspPI GGATC 2 cut(s) 185, 198
BssECI CCNNGG 2 cut(s) 154, 230
BssMI GATC 4 cut(s) 114, 190, 199, 216
Bst2UI CCWGG 2 cut(s) 50, 232
BstC8I GCNNGC 1 cut(s) 65
BstEII GGTNACC 1 cut(s) 130
BstF5I GGATG 1 cut(s) 9
BstFNI CGCG 1 cut(s) 30
BstH2I RGCGCY 1 cut(s) 70
BstHHI GCGC 4 cut(s) 9, 63, 69, 214
BstKTI GATC 4 cut(s) 117, 193, 202, 219
BstMBI GATC 4 cut(s) 114, 190, 199, 216
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 2 cut(s) 27, 69
BstNI CCWGG 2 cut(s) 50, 232
BstNSI RCATGY 1 cut(s) 145
BstPI GGTNACC 1 cut(s) 130
BstSCI CCNGG 3 cut(s) 48, 172, 230
BstSLI GKGCMC 1 cut(s) 23
BstUI CGCG 1 cut(s) 30
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BsuRI GGCC 2 cut(s) 21, 228
BtsCI GGATG 1 cut(s) 9
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 147
Cac8I GCNNGC 1 cut(s) 65
CfoI GCGC 4 cut(s) 9, 63, 69, 214
Cfr13I GGNCC 3 cut(s) 19, 20, 227
CseI GACGC 1 cut(s) 19
Csp6I GTAC 2 cut(s) 223, 238
CviAII CATG 4 cut(s) 73, 100, 142, 160
CviJI RGCY 4 cut(s) 21, 46, 164, 228
CviKI_1 RGCY 4 cut(s) 21, 46, 164, 228
CviQI GTAC 2 cut(s) 223, 238
DpnI GATC 4 cut(s) 116, 192, 201, 218
DpnII GATC 4 cut(s) 114, 190, 199, 216
EciI GGCGGA 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 23
Eco91I GGTNACC 1 cut(s) 130
EcoO109I RGGNCCY 2 cut(s) 19, 20
EcoO65I GGTNACC 1 cut(s) 130
EcoRII CCWGG 2 cut(s) 48, 230
EcoT38I GRGCYC 1 cut(s) 23
FaeI CATG 4 cut(s) 76, 103, 145, 163
FaiI YATR 4 cut(s) 74, 101, 143, 161
FaqI GGGAC 1 cut(s) 68
FatI CATG 4 cut(s) 72, 99, 141, 159
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 1 cut(s) 70
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 1 cut(s) 70
FspBI CTAG 1 cut(s) 241
GlaI GCGC 4 cut(s) 8, 62, 68, 213
GluI GCNGC 1 cut(s) 70
GsuI CTGGAG 2 cut(s) 32, 188
HaeII RGCGCY 1 cut(s) 70
HaeIII GGCC 2 cut(s) 21, 228
HapII CCGG 1 cut(s) 174
HgaI GACGC 1 cut(s) 19
HhaI GCGC 4 cut(s) 9, 63, 69, 214
Hin1II CATG 4 cut(s) 76, 103, 145, 163
Hin6I GCGC 4 cut(s) 7, 61, 67, 212
HinP1I GCGC 4 cut(s) 7, 61, 67, 212
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 2 cut(s) 92, 177
HpaII CCGG 1 cut(s) 174
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 1 cut(s) 199
Hpy188III TCNNGA 3 cut(s) 13, 181, 205
Hpy8I GTNNAC 1 cut(s) 124
Hpy99I CGWCG 3 cut(s) 92, 113, 125
HpyAV CCTTC 2 cut(s) 10, 178
HpyF10VI GCNNNNNNNGC 2 cut(s) 27, 69
Hsp92II CATG 4 cut(s) 76, 103, 145, 163
HspAI GCGC 4 cut(s) 7, 61, 67, 212
Kzo9I GATC 4 cut(s) 114, 190, 199, 216
LmnI GCTCC 2 cut(s) 51, 58
LpnPI CCDG 6 cut(s) 26, 35, 62, 187, 217, 218
LweI GCATC 1 cut(s) 18
MaeI CTAG 1 cut(s) 241
MaeIII GTNAC 1 cut(s) 130
MalI GATC 4 cut(s) 116, 192, 201, 218
MboI GATC 4 cut(s) 114, 190, 199, 216
MboII GAAGA 1 cut(s) 115
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 1 cut(s) 23
MlyI GAGTC 2 cut(s) 101, 186
MnlI CCTC 7 cut(s) 46, 49, 50, 146, 164, 205, 228
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 3 cut(s) 50, 174, 232
MvaI CCWGG 2 cut(s) 50, 232
MvnI CGCG 1 cut(s) 30
MwoI GCNNNNNNNGC 2 cut(s) 27, 69
NciI CCSGG 1 cut(s) 174
NdeII GATC 4 cut(s) 114, 190, 199, 216
NlaIII CATG 4 cut(s) 76, 103, 145, 163
NlaIV GGNNCC 4 cut(s) 21, 22, 192, 229
NmeAIII GCCGAG 1 cut(s) 135
NmuCI GTSAC 1 cut(s) 130
NspI RCATGY 1 cut(s) 145
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PflFI GACNNNGTC 1 cut(s) 80
PfoI TCCNGGA 2 cut(s) 48, 172
PkrI GCNGC 1 cut(s) 71
Ple19I CGATCG 1 cut(s) 219
PleI GAGTC 2 cut(s) 100, 185
PpsI GAGTC 2 cut(s) 100, 185
Psp6I CCWGG 2 cut(s) 48, 230
PspEI GGTNACC 1 cut(s) 130
PspGI CCWGG 2 cut(s) 48, 230
PspN4I GGNNCC 4 cut(s) 21, 22, 192, 229
PspOMI GGGCCC 1 cut(s) 19
PspPI GGNCC 3 cut(s) 19, 20, 227
PsuI RGATCY 1 cut(s) 190
PsyI GACNNNGTC 1 cut(s) 80
PvuI CGATCG 1 cut(s) 219
RsaI GTAC 2 cut(s) 224, 239
RsaNI GTAC 2 cut(s) 223, 238
SalI GTCGAC 1 cut(s) 122
SatI GCNGC 1 cut(s) 70
Sau3AI GATC 4 cut(s) 114, 190, 199, 216
Sau96I GGNCC 3 cut(s) 19, 20, 227
SchI GAGTC 2 cut(s) 101, 186
ScrFI CCNGG 3 cut(s) 50, 174, 232
SduI GDGCHC 1 cut(s) 23
SetI ASST 4 cut(s) 48, 132, 189, 239
SfaNI GCATC 1 cut(s) 18
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
SsiI CCGC 3 cut(s) 28, 70, 96
SspMI CTAG 1 cut(s) 241
StyD4I CCNGG 3 cut(s) 48, 172, 230
TaqI TCGA 2 cut(s) 123, 138
TaqII GACCGA 1 cut(s) 122
TauI GCSGC 1 cut(s) 72
TscAI CASTG 1 cut(s) 154
TseFI GTSAC 1 cut(s) 130
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 2 cut(s) 17, 116
TspRI CASTG 1 cut(s) 154
Tth111I GACNNNGTC 1 cut(s) 80
XceI RCATGY 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 123
XspI CTAG 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.