Rh1DG023100

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0019101
Physical Location & Seq
Forward (+)
3582566 .. 3592208
9643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG023100.1

Sequence Viewer

Length: 1035 bp
ATGGCAGTGGGAGAGATCTTTCTTGCGGCATTCCTTCAGTTGCTGCTGGACAGGTTAACGCCTGGGGAGATCCTGGGCTACTTTGGAAGCTTGGGAGGAGTTGGCAAAGAGCTGCAGAAATGGAGGGGAACACTGTCCGCAATTGCAGCGGTGCTTTCCGATGCGGAGGAGAAGCAGCTGACGAGCAAGGCAGTGGATCTGTGGCTGAATGACCTGAAACACTTGGCTTATGATCTGGACGATCTACTCAACACCTTTTCTAATGAGATGTTAGAACGCGAGCAGCTCAAGCTTCGCCAAACTGGCACAAGCAAGGTACGAGGCTTCTTTACCGAAGTTCCTCACAAAGTTAAGTTCAATTTCAATATGAACTCCGAAGTAGACGAGATTGCTAATCGGTTGCAAGACATAGTTGACCGGAAAGAGAAACTCGGTTTGAAATATATTGAGCAAACCTCTACGTCAACATCGTCATCGAAAAGGACACTAAGTTCGCACCTGCCAGTTGGAACTGTGGTTGGGAGAGATGAAGACGCAAGAAAGATTGTGGATCTGTTGTCCAAAGATGTTCATCCTTCTTCTACCACCAACTATGAAGTTGTTGCCATTGTTGTTTCTGTCTTCAAATGTCAAGGAAACAGCTGCCTGTATAAGGATGAGAAAGCCATTAGGCAATGGACCACCTCGAGATCTGGTTTCTCTATTTTAAGGCTACAGTGCACTGTGTTATTCCGATGGTCTGTTGCATATGAAAACCGGGTGCTGGAGCTGTTGGGGCCACTGGAACTCTTAAAGGGCGTTGGAGCAGAAGAATTTATGTGCAGTTGTTCAGGAAAGCATGATACTATCATCATCTTAGCCGTATTTATAAGTACTCGGTCAAAAAATGCTATTCAATACAGAAAAGCCGAAGAATGTATGTGCAACTGTTCAAGAGCCGAGGTACAAGAGGGTCATGCTAGCATAAAATCATCGACTGCTGGAGAGCAACGCCTGAATACATCTTTGGCTGTGTTTTATAGTATCAGGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

344

Amino Acids

38.49

Weight (kDa)

7.58

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 99 3.9e-19 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019101)

Species Orthologous Gene IDs
rosa_roxburghii Rroxscaffold_160G00433630 Rroxscaffold_4G00328140
rosa_rugosa Rorug01G0055800
rosa_samantha Rh1BG039100 Rh1CG042700 Rh1DG023100 Rh1DG024800 Rh1DG026900
rosa_wichuraiana Rw1G003590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 869
AarI CACCTGC 1 cut(s) 507
Acc36I ACCTGC 1 cut(s) 507
AccI GTMKAC 1 cut(s) 381
AccII CGCG 1 cut(s) 279
AciI CCGC 4 cut(s) 26, 138, 149, 164
AclWI GGATC 3 cut(s) 64, 204, 558
AcsI RAATTY 1 cut(s) 812
AcuI CTGAAG 1 cut(s) 20
AfaI GTAC 3 cut(s) 318, 874, 945
AfiI CCNNNNNNNGG 2 cut(s) 652, 763
AgsI TTSAA 6 cut(s) 358, 364, 439, 625, 896, 933
AjnI CCWGG 2 cut(s) 61, 72
AjuI GAANNNNNNNTTGG 2 cut(s) 989, 1021
AloI GAACNNNNNNTCC 2 cut(s) 475, 507
AluBI AGCT 7 cut(s) 90, 112, 178, 286, 292, 642, 769
AluI AGCT 7 cut(s) 90, 112, 178, 286, 292, 642, 769
Alw21I GWGCWC 1 cut(s) 722
Alw44I GTGCAC 1 cut(s) 718
AlwI GGATC 3 cut(s) 64, 204, 558
AlwNI CAGNNNCTG 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 685
AoxI GGCC 1 cut(s) 776
ApaLI GTGCAC 1 cut(s) 718
ApeKI GCWGC 6 cut(s) 43, 112, 146, 175, 283, 642
ApoI RAATTY 1 cut(s) 812
ArsI GACNNNNNNTTYG 2 cut(s) 475, 507
AspS9I GGNCC 2 cut(s) 678, 776
AsuC2I CCSGG 1 cut(s) 758
AsuNHI GCTAGC 1 cut(s) 959
AvaI CYCGRG 1 cut(s) 685
AvaII GGWCC 1 cut(s) 678
BaeGI GKGCMC 1 cut(s) 722
BbsI GAAGAC 2 cut(s) 537, 613
Bbv12I GWGCWC 1 cut(s) 722
BbvI GCAGC 6 cut(s) 30, 99, 158, 187, 295, 629
BccI CCATC 1 cut(s) 729
BceAI ACGGC 1 cut(s) 845
BciT130I CCWGG 2 cut(s) 63, 74
BcnI CCSGG 1 cut(s) 758
BfaI CTAG 1 cut(s) 960
BfmI CTRYAG 2 cut(s) 113, 713
BfuAI ACCTGC 1 cut(s) 507
BglI GCCNNNNNGGC 1 cut(s) 303
BglII AGATCT 2 cut(s) 15, 689
BisI GCNGC 7 cut(s) 27, 44, 113, 147, 176, 284, 643
BlsI GCNGC 7 cut(s) 28, 45, 114, 148, 177, 285, 644
BmcAI AGTACT 1 cut(s) 874
Bme1390I CCNGG 3 cut(s) 63, 74, 758
Bme18I GGWCC 1 cut(s) 678
BmeT110I CYCGRG 1 cut(s) 685
BmgT120I GGNCC 2 cut(s) 678, 776
BmiI GGNNCC 1 cut(s) 777
BmrFI CCNGG 3 cut(s) 63, 74, 758
BmsI GCATC 1 cut(s) 151
BmtI GCTAGC 1 cut(s) 963
BpiI GAAGAC 2 cut(s) 537, 613
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 2 cut(s) 785, 1002
BpuEI CTTGAG 1 cut(s) 272
BpuMI CCSGG 1 cut(s) 758
BsaBI GATNNNNATC 1 cut(s) 570
BsaJI CCNNGG 3 cut(s) 62, 73, 939
BsaWI WCCGGW 1 cut(s) 417
Bsc4I CCNNNNNNNGG 2 cut(s) 652, 763
Bse1I ACTGG 3 cut(s) 307, 503, 786
Bse3DI GCAATG 1 cut(s) 680
Bse8I GATNNNNATC 1 cut(s) 570
BseBI CCWGG 2 cut(s) 63, 74
BseDI CCNNGG 3 cut(s) 62, 73, 939
BseGI GGATG 2 cut(s) 571, 661
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 2 cut(s) 652, 763
BseMI GCAATG 1 cut(s) 680
BseNI ACTGG 3 cut(s) 307, 503, 786
BseRI GAGGAG 2 cut(s) 111, 182
BseSI GKGCMC 1 cut(s) 722
BseXI GCAGC 6 cut(s) 30, 99, 158, 187, 295, 629
BsgI GTGCAG 1 cut(s) 841
Bsh1236I CGCG 1 cut(s) 279
BshFI GGCC 1 cut(s) 778
BsiHKAI GWGCWC 1 cut(s) 722
BsiHKCI CYCGRG 1 cut(s) 685
BsiSI CCGG 2 cut(s) 418, 757
BslI CCNNNNNNNGG 2 cut(s) 652, 763
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 1 cut(s) 778
BsoBI CYCGRG 1 cut(s) 685
Bsp1286I GDGCHC 1 cut(s) 722
Bsp143I GATC 7 cut(s) 15, 69, 196, 232, 241, 550, 689
BspACI CCGC 4 cut(s) 26, 138, 149, 164
BspANI GGCC 1 cut(s) 778
BspFNI CGCG 1 cut(s) 279
BspLI GGNNCC 1 cut(s) 777
BspMAI CTGCAG 1 cut(s) 117
BspMI ACCTGC 1 cut(s) 507
BspOI GCTAGC 1 cut(s) 963
BspPI GGATC 3 cut(s) 64, 204, 558
BsrDI GCAATG 1 cut(s) 680
BsrI ACTGG 3 cut(s) 307, 503, 786
BssECI CCNNGG 3 cut(s) 62, 73, 939
BssMI GATC 7 cut(s) 15, 69, 196, 232, 241, 550, 689
Bst2UI CCWGG 2 cut(s) 63, 74
Bst4CI ACNGT 5 cut(s) 135, 514, 717, 724, 929
BstC8I GCNNGC 2 cut(s) 281, 961
BstDEI CTNAG 2 cut(s) 488, 856
BstENI CCTNNNNNAGG 1 cut(s) 650
BstF5I GGATG 2 cut(s) 571, 661
BstFNI CGCG 1 cut(s) 279
BstKTI GATC 7 cut(s) 18, 72, 199, 235, 244, 553, 692
BstMBI GATC 7 cut(s) 15, 69, 196, 232, 241, 550, 689
BstMWI GCNNNNNNNGC 4 cut(s) 146, 289, 303, 775
BstNI CCWGG 2 cut(s) 63, 74
BstSCI CCNGG 3 cut(s) 61, 72, 756
BstSFI CTRYAG 2 cut(s) 113, 713
BstSLI GKGCMC 1 cut(s) 722
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 6 cut(s) 30, 99, 158, 187, 295, 629
BstV2I GAAGAC 2 cut(s) 537, 613
BstX2I RGATCY 5 cut(s) 15, 69, 196, 550, 689
BstYI RGATCY 5 cut(s) 15, 69, 196, 550, 689
BsuRI GGCC 1 cut(s) 778
BtsCI GGATG 2 cut(s) 571, 661
BtsI GCAGTG 2 cut(s) 12, 198
BtsIMutI CAGTG 6 cut(s) 12, 131, 198, 720, 722, 779
BveI ACCTGC 1 cut(s) 507
Cac8I GCNNGC 2 cut(s) 281, 961
CaiI CAGNNNCTG 1 cut(s) 43
Cfr13I GGNCC 2 cut(s) 678, 776
CseI GACGC 1 cut(s) 542
Csp6I GTAC 3 cut(s) 317, 873, 944
CviAII CATG 2 cut(s) 839, 956
CviQI GTAC 3 cut(s) 317, 873, 944
DdeI CTNAG 2 cut(s) 488, 856
DpnI GATC 7 cut(s) 17, 71, 198, 234, 243, 552, 691
DpnII GATC 7 cut(s) 15, 69, 196, 232, 241, 550, 689
Eco47I GGWCC 1 cut(s) 678
Eco57I CTGAAG 1 cut(s) 20
Eco88I CYCGRG 1 cut(s) 685
EcoNI CCTNNNNNAGG 1 cut(s) 650
EcoRII CCWGG 2 cut(s) 61, 72
FaeI CATG 2 cut(s) 842, 959
FatI CATG 2 cut(s) 838, 955
FauNDI CATATG 1 cut(s) 748
FblI GTMKAC 1 cut(s) 381
Fnu4HI GCNGC 7 cut(s) 27, 44, 113, 147, 176, 284, 643
FokI GGATG 2 cut(s) 558, 668
Fsp4HI GCNGC 7 cut(s) 27, 44, 113, 147, 176, 284, 643
FspBI CTAG 1 cut(s) 960
GluI GCNGC 7 cut(s) 27, 44, 113, 147, 176, 284, 643
GsuI CTGGAG 2 cut(s) 785, 1002
HaeIII GGCC 1 cut(s) 778
HapII CCGG 2 cut(s) 418, 757
HgaI GACGC 1 cut(s) 542
Hin1II CATG 2 cut(s) 842, 959
HincII GTYRAC 3 cut(s) 57, 415, 465
HindII GTYRAC 3 cut(s) 57, 415, 465
HindIII AAGCTT 2 cut(s) 88, 290
HpaI GTTAAC 1 cut(s) 57
HpaII CCGG 2 cut(s) 418, 757
Hpy166II GTNNAC 5 cut(s) 57, 382, 415, 465, 720
Hpy188I TCNGA 3 cut(s) 160, 376, 734
Hpy188III TCNNGA 4 cut(s) 236, 687, 831, 933
Hpy8I GTNNAC 5 cut(s) 57, 382, 415, 465, 720
HpyAV CCTTC 2 cut(s) 44, 585
HpyCH4III ACNGT 5 cut(s) 135, 514, 717, 724, 929
HpyCH4IV ACGT 1 cut(s) 461
HpyCH4V TGCA 7 cut(s) 115, 146, 403, 720, 746, 822, 924
HpyF10VI GCNNNNNNNGC 4 cut(s) 146, 289, 303, 775
HpyF3I CTNAG 2 cut(s) 488, 856
HpySE526I ACGT 1 cut(s) 461
Hsp92II CATG 2 cut(s) 842, 959
KspAI GTTAAC 1 cut(s) 57
Kzo9I GATC 7 cut(s) 15, 69, 196, 232, 241, 550, 689
LmnI GCTCC 2 cut(s) 766, 803
Lsp1109I GCAGC 6 cut(s) 30, 99, 158, 187, 295, 629
LweI GCATC 1 cut(s) 151
MaeI CTAG 1 cut(s) 960
MaeII ACGT 1 cut(s) 461
MalI GATC 7 cut(s) 17, 71, 198, 234, 243, 552, 691
MboI GATC 7 cut(s) 15, 69, 196, 232, 241, 550, 689
MboII GAAGA 5 cut(s) 542, 570, 613, 821, 923
MfeI CAATTG 1 cut(s) 141
MflI RGATCY 5 cut(s) 15, 69, 196, 550, 689
MhlI GDGCHC 1 cut(s) 722
MluCI AATT 3 cut(s) 141, 358, 812
MmeI TCCRAC 2 cut(s) 487, 781
MnlI CCTC 9 cut(s) 89, 117, 160, 314, 351, 466, 694, 934, 943
MseI TTAA 4 cut(s) 56, 351, 707, 791
MspA1I CMGCKG 3 cut(s) 149, 178, 642
MspI CCGG 2 cut(s) 418, 757
MspR9I CCNGG 3 cut(s) 63, 74, 758
MunI CAATTG 1 cut(s) 141
Mva1269I GAATGC 1 cut(s) 29
MvaI CCWGG 2 cut(s) 63, 74
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 4 cut(s) 146, 289, 303, 775
NciI CCSGG 1 cut(s) 758
NdeI CATATG 1 cut(s) 748
NdeII GATC 7 cut(s) 15, 69, 196, 232, 241, 550, 689
NheI GCTAGC 1 cut(s) 959
NlaIII CATG 2 cut(s) 842, 959
NlaIV GGNNCC 1 cut(s) 777
NmeAIII GCCGAG 1 cut(s) 964
PaeR7I CTCGAG 1 cut(s) 685
PaqCI CACCTGC 1 cut(s) 507
PcsI WCGNNNNNNNCGW 1 cut(s) 467
PctI GAATGC 1 cut(s) 29
PkrI GCNGC 7 cut(s) 28, 45, 114, 148, 177, 285, 644
PsiI TTATAA 1 cut(s) 869
Psp6I CCWGG 2 cut(s) 61, 72
PspGI CCWGG 2 cut(s) 61, 72
PspN4I GGNNCC 1 cut(s) 777
PspPI GGNCC 2 cut(s) 678, 776
PstI CTGCAG 1 cut(s) 117
PstNI CAGNNNCTG 1 cut(s) 43
PsuI RGATCY 5 cut(s) 15, 69, 196, 550, 689
PvuII CAGCTG 2 cut(s) 178, 642
RsaI GTAC 3 cut(s) 318, 874, 945
RsaNI GTAC 3 cut(s) 317, 873, 944
SaqAI TTAA 4 cut(s) 56, 351, 707, 791
SatI GCNGC 7 cut(s) 27, 44, 113, 147, 176, 284, 643
Sau3AI GATC 7 cut(s) 15, 69, 196, 232, 241, 550, 689
Sau96I GGNCC 2 cut(s) 678, 776
ScaI AGTACT 1 cut(s) 874
ScrFI CCNGG 3 cut(s) 63, 74, 758
SduI GDGCHC 1 cut(s) 722
SfaNI GCATC 1 cut(s) 151
SfcI CTRYAG 2 cut(s) 113, 713
Sfr274I CTCGAG 1 cut(s) 685
SinI GGWCC 1 cut(s) 678
SlaI CTCGAG 1 cut(s) 685
SmlI CTYRAG 2 cut(s) 287, 685
SmoI CTYRAG 2 cut(s) 287, 685
Sse9I AATT 3 cut(s) 141, 358, 812
SsiI CCGC 4 cut(s) 26, 138, 149, 164
SspMI CTAG 1 cut(s) 960
StyD4I CCNGG 3 cut(s) 61, 72, 756
TaaI ACNGT 5 cut(s) 135, 514, 717, 724, 929
TaiI ACGT 1 cut(s) 464
TaqI TCGA 3 cut(s) 476, 686, 974
TaqII GACCGA 1 cut(s) 867
TasI AATT 3 cut(s) 141, 358, 812
TatI WGTACW 1 cut(s) 872
TauI GCSGC 1 cut(s) 29
Tru1I TTAA 4 cut(s) 56, 351, 707, 791
Tru9I TTAA 4 cut(s) 56, 351, 707, 791
TscAI CASTG 6 cut(s) 12, 138, 198, 722, 727, 786
TseI GCWGC 6 cut(s) 43, 112, 146, 175, 283, 642
TspDTI ATGAA 5 cut(s) 383, 543, 560, 609, 765
TspRI CASTG 6 cut(s) 12, 138, 198, 722, 727, 786
VneI GTGCAC 1 cut(s) 718
VpaK11BI GGWCC 1 cut(s) 678
XagI CCTNNNNNAGG 1 cut(s) 650
XapI RAATTY 1 cut(s) 812
XhoI CTCGAG 1 cut(s) 685
XmiI GTMKAC 1 cut(s) 381
XspI CTAG 1 cut(s) 960
ZrmI AGTACT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.